JPH0933402A - Microscopic specimen sampling needle and manufacture thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a microscopic specimen sampling needle which enables the sampling of a microscopic specimen with the diameter of about 3μm by arranging a sharp needle tip and a cut. SOLUTION: In a microscopic specimen sampling needle 1, a needle tip 5 exists at one point on the outer circumference 1Sb of the tip of a metal bar with a circular cross section. The needle point 5 is set at a top thereof and inclined cut openings 4A and 4B are formed in such a manner to rise gradually on both sides thereof and then, retract. Blades 6A and 6B are formed by edges Ld and Le at which the inclined cut openings 4A and 4B cross the outer circumference 1Sb. A blade is also formed by the edge Lc which both the inclined cuts 4A and 4B cross.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sampling needle for collecting a micro sample used for micro analysis and a method for manufacturing the same.

[0002]

2. Description of the Related Art Techniques such as a microscopic Fourier transform infrared spectrophotometer, a microscopic Raman spectrophotometer, a mass spectroscope, and a micro X-ray diffractometer are used for material analysis of minute amounts of minute substances. Along with the improvement in sensitivity of these various analyzers and miniaturization of products such as magnetic recording media and electronic parts, the size of samples to be analyzed has become minute. For example, in the microscopic Fourier transform infrared spectrophotometer, the diameter is 3 μm.
It is becoming possible to analyze even a sample of a minute size. In addition, the shape of the micro sample is not limited to a spherical shape, but may be a cube, a strip shape, a needle shape, or the like. Here, for simplification of description, it is assumed that the micro sample is “spherical” and “diameter”. Will be used.

In the case of such an analysis, in most cases, it is possible to obtain a better result by separating and collecting a micro sample. If the sample is about 100 μm in diameter, separate it using tweezers, needles, etc. under a stereomicroscope.
Sampling is possible, but a manipulator is required to separate and collect a micro sample of a size smaller than this.

FIG. 6 is a schematic diagram showing the structure of a general manipulator 20. Reference numeral 21 is a sample table whose mounting surface 21A is horizontal, and by rotating the knob 22 in the horizontal plane, the sample table 21 is rotated, for example, in the X-axis (left-right) direction by rotating the knob 23. The sample table 21 can be finely moved in the Y-axis (front-back) direction. Code 2
Reference numeral 4 denotes a support rod, one end of which is fixed to one side surface of the sample table 21 and a connecting metal fitting 25 is fixed to the other end thereof. The connecting metal fitting 25 is further vertically supported by the support rod 24. One end of 26 is fixed.

At the other end of the support rod 26, a sliding device 28 consisting of a slider 28A sliding vertically and a stator 28B.
The stator 28B is fixed via a connecting fitting 27. A knob 29 is attached to the sliding device 28, and by rotating the knob 29, the slider 28A can be finely adjusted in the Z-axis (vertical) direction. A stator 30B of a sliding device 30 including a slider 30A and a stator 30B is fixed to the slider 28A.
A knob 31 is attached to the sliding device 30, and by rotating the knob 31, the slider 30A can be finely adjusted in the Y-axis (front-back) direction. A stator 32B of a sliding device 32 including a slider 32A and a stator 32B is fixed to the slider 30A via a spacer. A knob 33 is attached to the sliding device 32. By rotating the knob 33, the slider 32A is moved to the X axis (left and right).
The structure allows fine adjustment in the direction.

A slide device 35 is fixed on the slider 32A via a support rod 34, and a support device 37 of a needle holding device 38 is fixed on the coarse adjustment device 35 via a support rod 36. Has been done. The needle holding device 38 is composed of a support rod 38A fixed to the supporting device 37, an angle adjusting device 38B supported by this tip portion, and a needle holding rod 38C.

In order to separate and collect a minute sample to be analyzed using the manipulator 20 having such a structure,
First, a product on which a minute sample P to be separated and collected is present on the mounting surface 21A of the sample table 21, for example, a magnetic tape T of a section is placed, and the knobs 22 and 23 are operated to make a small amount on the tip of the needle 40. The sample table 21 is moved so that the sample P comes. Next, the rough adjusting device 35, the knobs 29, 31, 33 and the angle adjusting device 38B are operated to move the needle tip of the needle 40 to the X-axis and Y-axis.
By moving in the direction of the axis and the Z axis, separating and collecting the micro sample P,
Transfer to a prepared sample stand dedicated to the analyzer. It should be noted that the separation and collection of the micro sample P is performed by using the microscope 50, and a knife or the like can be attached instead of the needle 40 to perform cutting, peeling, perforation, chemical injection, and the like.

[0008]

In order to separate and collect the minute sample P, in many cases, the needle tip 41 as shown in FIG. 7 is placed in the center of the needle body 42 when viewed from the front of the needle 40. It is performed using a needle with a round needle tip. However, the needle 40 having such a structure can deal only with a relatively large and lightly adhered micro sample up to a diameter of about 20 μm. Recently, the samples to be analyzed have become smaller and smaller, and the adhesion state has become more complicated. For small samples with a diameter of less than 20 μm, small samples that are thinly attached, small samples that are embedded, etc. Have difficulty.

It is considered that this is due to insufficient sharpness of the needle tip and its shape. That is, a sharp needle tip cannot be obtained by an ordinary method for manufacturing a needle, and conversely, a sharp needle causes a problem such as a decrease in strength. Further, as shown in FIG. 7, the round tip shape of the needle tip has almost no function of peeling a minute sample. Further, even though the analyzer has a sufficient analysis capability, it may not be possible to collect a minute sample of the sample for analysis. Therefore, an object of the present invention is to solve these problems, and an object thereof is to obtain a micro sampling needle having a sharp needle tip and a blade, and a method for manufacturing the same.

[0010]

Therefore, according to the micro sampling needle of the present invention, the needle tip is present at one point of the arc-shaped outer periphery of the tip of the rod having at least a part of the outer periphery formed in the arc shape. With the tip of the needle as the apex, the sloped cuts that gradually recede on both sides thereof and recede are formed, and a blade is formed by a ridge line where each of the sloped cuts and the arcuate outer periphery intersect,
Further, the above problem was solved by configuring the blade so as to form a ridge line at which the both inclined cuts intersect.

Further, in the method for manufacturing a micro sampling needle according to the present invention, the tip portion of a rod having at least a part of its outer periphery formed in an arc shape is formed at a certain minute angle with respect to the longitudinal direction of the rod main body. An inclined surface is formed by cutting from the outer peripheral surface opposite to the arcuate outer periphery so that the tip constitutes a needle point existing on the arcuate outer peripheral surface of the rod main body, and further inclined from both side surfaces of the inclined surface. The micro sampling needle was manufactured by a method of cutting in a direction intersecting with each other toward the center line of the surface and with inclined surfaces diverging toward each other.

Therefore, since the micro sample collecting needle of the present invention is provided with a sharp needle tip, it is possible to collect a micro sample having a diameter of up to about 3 μm, and a micro sample thinly attached by using a blade. It became possible to peel off and collect. Furthermore, it has become possible to collect embedded micro-samples using the needle tip and blade. Further, when the method for manufacturing a micro sample collecting needle of the present invention is adopted, the micro sample collecting needle can be obtained by an extremely simple processing step.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION Next, referring to FIGS. 1 to 5, a micro sampling needle of the present invention and a method for manufacturing the same will be described. FIG. 1 shows a metal rod main body which is a starting material of a method for manufacturing a micro sampling needle according to an embodiment of the present invention.
1 is a side view thereof, FIG. 2B is a front view thereof, and FIG.
The metal rod main body shown in FIG. 2A is an intermediately processed metal rod main body, and FIG.
FIG. 3B is a top view of the same, and FIG. 3C is a front view as seen from its tip. FIG. 3 is obtained by subjecting the metal rod body in the intermediately processed state shown in FIG. 2 to the second stage processing. FIG. 4 is a side view of FIG. A, a top view of FIG. B, and a front view of the needle tip of FIG.
Shows a metal rod body which is another starting material that can be used in the method for manufacturing a micro sampling needle of the present invention, FIG. A is a side view thereof, and FIG. B is a front view thereof, and 5 shows a micro sampling needle of another shape of the present invention manufactured using the starting material of FIG. 4, FIG. 5A is a side view thereof, FIG. 5B is a top view thereof, and FIG. It is a front view which shows the needle point.

In FIG. 1, reference numeral 1M is a first starting material for manufacturing the micro-sampling needle of the present invention. For example, a metal rod body such as stainless steel having a diameter of 1 mm which is hard to rust and is rigid. Is. This metal rod body 1M
Is a rod with a circular cross section formed with the same diameter over its entire length. The term "metal rod" used in the present specification includes, in addition to the rod having a solid central portion as described above, a rod having a hollow central portion as described later, that is, a pipe-shaped rod. Note that sticks are included.

The metal rod body 1M having such a shape is shown in FIG.
A first stage cutting process is performed along the alternate long and short dash line La shown in FIG. That is, one tip portion of the metal rod body 1M is processed from one outer peripheral surface 1Sa of the metal rod body 1M to the other outer peripheral surface 1Sb, and is cut at a certain minute angle θa with respect to the longitudinal direction thereof to form an inclined surface. Form 2. The metal rod body 1M that has been subjected to this cutting process is referred to as an intermediate product 1T.
The tip 3 of this intermediate product 1T is not yet sharp and pointed.

Next, as shown in FIG. 2B, the intermediate product 1T intersects from both side surfaces of the inclined surface 2 toward the center line Lo of the tip portion thereof, and the one outer peripheral surface 1Sa side is The cutting is performed along the alternate long and short dash line Lb having the minute angle ± θb so that the outer peripheral surface 1Sb side is narrower and the other outer peripheral surface 1Sb side is symmetric.

Then, the micro sample collecting needle 1 of the present invention having the structure shown in FIG. 3 is obtained. That is, FIG. 3A, B
As shown in FIG.
Sloping cuts 4A, 4
B is formed, and a sharp needle tip 5 is formed at the tip of the ridge line Lc where these cuts 4A, 4B intersect. 3A,
As is clear from C, the needle tip 5 is the other outer peripheral surface 1
It is on Sb. Further, the ridge lines Ld and Le that intersect the cutting edges 4A and 4B and the other outer peripheral surface 1Sb that obliquely retreat from the needle tip 5 to the inclined surface 2 along the outer circumference of the metal rod main body 1M are respectively defined. The sharp blades 6A and 6B are formed.

In other words, the micro sampling needle 1 of the present invention
Has a needle tip 5 at one point of the outer periphery 1Sb at the tip of the metal rod 1M having a circular cross section, and the needle tip 5 is set at the apex, and the inclined cuts 4A, 4A, 4A that gradually move upward and backward on both sides thereof.
B is formed, and the blades 6A are formed by ridge lines Ld and Le at which the respective inclined cuts 4A and 4B intersect the outer circumference 1Sb.
6B is formed, and further, the both inclined cuts 4 are formed.
A blade is also formed on the ridge line Lc where A and 4B intersect.
As described above, the micro sample collecting needle 1 of the present invention has a structure including the sharp needle tip 5 and the symmetrical sharp blades 6A, 6B and Lc having the needle tip 5 at the apex. There is.

The micro sampling needle 1 of the present invention is also shown in FIG.
Even if the pipe-shaped metal rod main body 1N as shown in FIG. Since the central portion of the pipe-shaped metal rod body 1N is hollow E, the inclined surface 2 and the cuts 4A and 4B can be easily cut, and the processing time can be shortened. In manufacturing the sampling needle, it is more advantageous than the rod-shaped body having a solid central portion like the metal rod body 1M.

Then, the micro sampling needle 1A of this embodiment is used.
In addition, through the same processing steps as the processing steps of the micro sample collecting needle 1 of the above-mentioned embodiment, the sharp needle tip 5 and the blades 6A and 6B similar to those of the micro sample collecting needle 1 can be formed. The difference in shape is only that the center of the micro sampling needle 1A is hollow E. Therefore, detailed description of the manufacturing process of the micro sample collecting needle 1A is omitted.

In each of the above embodiments, a metal rod having a circular cross section was used as an example of the starting material, and the structure of the micro sampling needle and the manufacturing method thereof were described. The starting material is a metal having a circular cross section. The rod need not be a rod, and may be, for example, a metal rod having an elliptical cross section, and the point is that the needle tip is located on the arc-shaped outer peripheral surface of the metal rod. Therefore, it is sufficient that the metal rod has an arc formed on a part of its outer periphery.
It can be easily understood from the above description. The material of the micro sample collecting needle of the present invention is not limited to "metal". Depending on the environment in which the sample to be collected exists, a plastic material or a bamboo material can be used as a starting material as long as it has a certain degree of rigidity.

[0022]

As described above, as a result of collecting the micro sample P using the sampling needle of the present invention, it was possible to separate and collect the micro sample P having a diameter of up to about 3 μm. Further, by using the blade of the sampling needle, the minute sample P that is thinly adhered can be easily peeled, separated and collected. In addition, the embedded micro sample P could be collected using the blade and the tip of the needle. Therefore, it has become possible to collect a large number of small samples that were previously difficult to collect, and it is now possible to take full advantage of the excellent analysis functions originally provided in the analyzer, and to obtain excellent analysis results. It is now possible to analyze the quality of products.

[Brief description of drawings]

FIG. 1 shows a metal rod main body which is a starting material of a method for manufacturing a micro sampling needle according to an embodiment of the present invention.
Is a side view thereof, and FIG. B is a front view thereof.

2 shows a semi-processed metal rod main body obtained by subjecting the metal rod main body shown in FIG. 1 to a first-stage processing, wherein FIG. 2A is a side view thereof, FIG. 2B is a top view thereof, and FIG. FIG. C is a front view seen from the tip.

FIG. 3 shows a micro sampling needle of the present invention obtained by subjecting a metal rod body in an intermediately processed state shown in FIG. 2 to a second stage processing, and FIG. 3A is a side view and FIG. B is a top view thereof, and FIG. C is a front view showing the needle tip thereof.

FIG. 4 shows a metal rod body which is another starting material that can be used in the method for manufacturing a micro sampling needle of the present invention, in which FIG. A is a side view and FIG. B is a front view thereof. is there.

5 shows another shape of the micro sampling needle of the present invention manufactured using the starting material of FIG. 4, FIG. 5A is a side view thereof, FIG. 5B is a top view thereof, and FIG. [Fig. 3] is a front view showing the tip of the needle.

FIG. 6 is a schematic diagram showing a structure of a general manipulator.

FIG. 7 shows a currently used micro sampling needle and is shown in FIG.
Is a side view thereof and FIG. B is a front view showing the needle tip thereof.

[Explanation of symbols]

 1 Micro Sample Collection Needle of First Example of the Present Invention 1A Micro Sample Collection Needle of Second Example of the Present Invention 1M Metal rod body 1N Pipe-shaped metal rod body 1Sa One outer peripheral surface of metal rod body 1Sb Metal rod Other outer peripheral surface of the main body 1T Intermediate product 2 Inclined surface 3 Tip portion 4A Cutting edge 4B Cutting edge 5 Needle tip 6A Blade 6B Blade Lc Both cutting edges 4A, 4B Ridge line Ld Cutting edge 4A and outer circumferential surface 1Sb Edge Le cutting edge 4B and outer circumferential surface Ridge line with 1Sb

Claims (2)

[Claims] 1. A needle tip is present at one point of the arc-shaped outer periphery of a tip of a rod having at least a part of the outer periphery formed in an arc shape,
Inclined cuts are formed on both sides of the needle tip as an apex, and gradually upward and backward, and a blade is formed at a ridge line at which each of the inclined cuts intersects the arcuate outer periphery, and further A micro sampling needle characterized in that a blade is also formed on a ridgeline where the two inclined cuts intersect. 2. A tip portion of a rod, at least a part of the outer periphery of which is formed in an arc shape, is cut from an outer peripheral surface opposite to the arc-shaped outer periphery at a small angle with respect to the longitudinal direction of the rod body. An inclined surface is formed so that the tip forms a needle point existing on the arc-shaped outer peripheral surface of the rod body, and further in a direction intersecting each other from both side surfaces of the inclined surface toward the center line of the inclined surface. And a method for manufacturing a micro sampling needle, characterized by cutting with sloping surfaces that diverge toward each other.