JPH09255462A - Culture soil for raising plant - Google Patents

Culture soil for raising plant

Info

Publication number
JPH09255462A
JPH09255462A JP6313196A JP6313196A JPH09255462A JP H09255462 A JPH09255462 A JP H09255462A JP 6313196 A JP6313196 A JP 6313196A JP 6313196 A JP6313196 A JP 6313196A JP H09255462 A JPH09255462 A JP H09255462A
Authority
JP
Japan
Prior art keywords
plant
soil
mycorrhizal
cake
fungus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6313196A
Other languages
Japanese (ja)
Other versions
JP3136265B2 (en
Inventor
Makoto Soda
良 曽田
Hirotaka Sato
裕隆 佐藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Forestry Co Ltd
Original Assignee
Sumitomo Forestry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Forestry Co Ltd filed Critical Sumitomo Forestry Co Ltd
Priority to JP6313196A priority Critical patent/JP3136265B2/en
Publication of JPH09255462A publication Critical patent/JPH09255462A/en
Application granted granted Critical
Publication of JP3136265B2 publication Critical patent/JP3136265B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cultivation Of Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Fertilizers (AREA)

Abstract

PROBLEM TO BE SOLVED: To promote the raising of a plant by mixing a water purifying cake having a specific phosphoric acid absorption coefficient and an available phosphoric acid with a versicular-arbuscular mycorrhizal(VA) fungus and forming the culture soil. SOLUTION: This culture soil for raisin a plant is contained by carrying out the disintegration and particle size selection of a water purifying cake, prepared by treating a sedimented muddy soil produced in a water purifying treating process according to a chemical nonfeeding method and having <=2000mg/100g phosphoric acid absorption coefficient, <=10mg/100g available phosphoric acid content, about 0.1-2.0% total phosphoric acid content and about >=0.3% total nitrogen content into about <=10mm diameter, than adding and mixing the water purifying cake disinfected with chlorine sterilization, etc., with about 5-50vol.% composing material such as a rice straw and about 5-50vol.% soil conditioning material such as coal, subsequently adding and mixing the resultant mixture with a VA fungus belonging to the Gigaspora, etc., in an amount so as to provide >=10 spores, preferably >=100 spores in 1L culture soil for raising the plant. A plant in which the VA fungus can be symbiotic such as a leguminous plant is cultivated in the culture soil for raising the plant to efficiently proliferate the VA fungus and further well grow the plant.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【発明の属する技術分野】本発明は、浄水ケーキにVA
菌根菌を混合してなる植物育成培土、この植物育成培土
を利用した植物育成方法、並びにVA菌根菌の増殖方法
に関する。更に詳細には、リン酸吸収係数が高く、有効
態リン酸が少ない土壌である浄水ケーキとVA菌根菌と
を組合わせて利用した植物育成培土、植物育成方法、並
びにVA菌根菌の増殖方法に関する。
TECHNICAL FIELD The present invention relates to a VA for a purified water cake.
TECHNICAL FIELD The present invention relates to a plant growing soil containing a mixture of mycorrhizal fungi, a plant growing method using the plant growing soil, and a method for growing VA mycorrhizal fungi. More specifically, a plant-cultivating soil, a plant-cultivating method, and a method for multiplying VA mycorrhizal fungi, which uses a combination of a water-purifying cake, which is a soil having a high phosphate absorption coefficient and low effective phosphate, and VA mycorrhizal fungi Regarding the method.

【0002】[0002]

【従来の技術】浄水場発生土もしくは浄水ケーキの農業
利用については、日本土壌肥料学会編(博友社)“浄水
処理ケーキ:特性と農業利用上の問題点”に詳細に記載
されている。浄水処理の過程で、凝集剤としてポリ塩化
アルミニウム、または液体硫酸アルミニウムが使用され
ているために、浄水ケーキ中に可溶性アルミニウムが多
量に含まれており、この活性アルミニウムが浄水ケーキ
のリン酸吸収係数を高め、浄水ケーキ中の有効態リン酸
を引き下げ、従って浄水ケーキを作物の栽培用に用いた
場合には作物のリン酸欠乏をきたす。このため浄水ケー
キを作物の育成培土として利用するためにリン酸吸収係
数に見合う量以上のリン酸を添加しなければならない。
従って、浄水ケーキを作物の栽培に利用するには難点が
ある。植物育成培土のうち緑化用の培土は一般に、土壌
の下層土に有機質、無機質の土壌改良材と肥料を混和し
たものを用いる。また、法面の吹き付け用の成育基盤材
は土壌、ピートモス、バーク堆肥等を適量混合したもの
が用いられ、それに肥料と種子、団粒化材等を混入し施
工される。緑化に用いられる肥料分は降雨などにより容
易に溶脱し、河川や湖沼の富栄養化等の環境汚染の一因
となっている。従って、このような環境汚染のない緑化
用の培土の開発が望まれている。
2. Description of the Related Art Agricultural utilization of soil produced by a water purification plant or purified water cake is described in detail in "Soil Purification Cake: Characteristics and Problems in Agricultural Utilization" edited by Japan Society of Soil Fertilizers (Hakuyusha). Since polyaluminum chloride or liquid aluminum sulfate is used as a coagulant in the process of water purification, the water purification cake contains a large amount of soluble aluminum, and this active aluminum is the phosphate absorption coefficient of the water purification cake. And lowers the effective phosphoric acid in the water purification cake, thus causing phosphate deficiency in the crop when the water purification cake is used for growing crops. Therefore, in order to use the purified water cake as the soil for growing crops, it is necessary to add more phosphoric acid than the phosphoric acid absorption coefficient.
Therefore, there is a difficulty in using the purified water cake for crop cultivation. Of the soil for growing plants, the soil for greening is generally a mixture of organic and inorganic soil improving materials and fertilizers in the soil subsoil. The growth base material for spraying the slope is a mixture of soil, peat moss, bark compost and the like in an appropriate amount, and is mixed with fertilizer, seeds, agglomerates and the like for construction. Fertilizer used for greening is easily leached due to rainfall, which is one of the causes of environmental pollution such as eutrophication of rivers and lakes. Therefore, it is desired to develop a soil for greening without such environmental pollution.

【0003】一方、VA菌根菌は藻菌類に属するカビの
一種であって、多くの植物にVA菌根を形成する菌であ
り、VA菌根菌は土壌中からリン酸やミネラルを吸収し
て植物体に供給する一方で、植物は菌の生育に必要な炭
水化物やアミノ酸などを提供してお互いに共生関係を維
持する絶体共生菌である。VA菌根菌は様々の植物に感
染し、その植物の生育を促進したり、植物の耐病性を向
上させることが一般に知られている。その効果のひとつ
に、土壌中に固定されたリン酸を植物に供給することが
挙げられる(「農業及び園芸」第62巻、第8号、P.
930−937、1987年)。
[0003] On the other hand, VA mycorrhizal fungus is a kind of fungus belonging to algal fungi and forms VA mycorrhiza in many plants. VA mycorrhizal fungus absorbs phosphate and minerals from soil. While supplying the plants with carbohydrates and amino acids necessary for the growth of the bacteria, the plants are symbiotic symbiotic bacteria that maintain a symbiotic relationship with each other. It is generally known that VA mycorrhizal fungi infect various plants, promote the growth of the plants, and improve the disease resistance of the plants. One of the effects is to supply the plant with phosphoric acid fixed in the soil ("Agriculture and Horticulture", Vol. 62, No. 8, P.
930-937, 1987).

【0004】しかし、VA菌根菌は絶対共生菌であるた
め純粋培養は不可能とされており、植物育成用に利用す
るために培養増殖するには植物との共生関係を利用する
以外にない。VA菌根菌を人工的に培養することは非常
に難しいとされており、その原因のひとつにVA菌根菌
を増殖させるためには宿主作物の生育を旺盛にさせる必
要があるが、宿主作物の生育に必要な肥料を与えるとV
A菌根菌の増殖が阻害されることがあげられる。VA菌
根菌の増殖法には、化成肥料を混合させる方法(特開平
3−76572号公報)や低温度の液肥を用いる方法
(特開昭55−118390号公報、特開昭60−23
7987号公報、特開昭62−19028号公報)、有
機質肥料を吸収させる方法(特開昭63−87973号
公報)、緩効性肥料を用いる方法(特開平6−2076
43号公報)等が知られている。これらの方法は肥料成
分の調製が煩雑で、植物の生育に応じて施肥回数が多く
なるなど手間がかかる等の欠点があった。
However, since VA mycorrhizal fungus is an absolute symbiotic bacterium, it cannot be purely cultivated. Therefore, the VA mycorrhizal fungus is cultivated and propagated for the purpose of growing the plant only by utilizing the symbiotic relationship with the plant. . It is said that it is very difficult to artificially culture VA mycorrhizal fungi, and one of the causes is to grow the host crop vigorously in order to grow VA mycorrhizal fungi. And fertilizer required for the growth of
The growth of A mycorrhizal fungi is inhibited. As a method for growing VA mycorrhizal fungi, a method of mixing a chemical fertilizer (JP-A-3-76572) or a method of using low temperature liquid fertilizer (JP-A-55-118390, JP-A-60-23).
7987, JP 62-19028), a method of absorbing organic fertilizer (JP 63-87973), and a method of using a slow-release fertilizer (JP 6-2076A).
No. 43) is known. These methods have drawbacks in that the preparation of fertilizer components is complicated and that the number of times fertilizer is applied increases according to the growth of plants, which is troublesome.

【0005】[0005]

【発明が解決しようとする課題】本発明者はこれらの従
来の問題点を解決すべく鋭意研究を進めた結果、VA菌
根菌を添加した浄水ケーキを植物育成培土として利用す
ることによって、VA菌根菌を効率良く増殖することが
でき、肥料を特に施用することなく植物が良好に生育
し、従って、VA菌根菌を添加した浄水ケーキが緑化用
の資材として有効であるという知見を得、この知見を基
に本発明を完成するに至った。従って、本発明の目的
は、浄水ケーキにVA菌根菌を添加することによって得
られる植物育成培土であって、VA菌根菌が効率良く増
殖し、肥料を特に施用することなく植物が生育すること
を可能とする植物育成培土、それを利用した植物育成方
法、並びにVA菌根菌の増殖方法を提供することにあ
る。
DISCLOSURE OF INVENTION Problems to be Solved by the Invention As a result of intensive studies to solve these conventional problems, the present inventor used VA mycorrhizal fungal purified water cake as a plant growing medium to obtain VA. It was found that the mycorrhizal fungi can be efficiently proliferated and the plants can grow well without applying fertilizers. Therefore, the water purification cake containing VA mycorrhizal fungi is effective as a greening material. The present invention has been completed based on this finding. Therefore, an object of the present invention is a plant-cultivating soil obtained by adding VA mycorrhizal fungi to a water-purified cake, in which VA mycorrhizal fungi grow efficiently and plants grow without applying fertilizers. It is intended to provide a plant cultivation medium that enables the above, a plant cultivation method using the same, and a method for multiplying VA mycorrhizal fungi.

【0006】[0006]

【課題を解決するための手段】本発明は、浄水ケーキに
VA菌根菌を混合してなる植物育成培土に関する。更に
本発明は、浄水ケーキにVA菌根菌を混合してなる植物
育成培土でVA菌根菌が共生しうる植物を栽培し、VA
菌根菌を植物に共生させてVA菌根菌を効率よく増殖さ
せて、植物を栽培することを特徴とする植物育成方法に
関する。更に本発明は、浄水ケーキからなる植物育成培
土にVA菌根菌を接種して、VA菌根菌が共生しうる植
物を栽培し、VA菌根菌を植物に共生させてVA菌根菌
を効率良く増殖させて、植物を栽培することを特徴とす
る植物育成方法に関する。更に本発明は、VA菌根菌が
共生しうる植物にVA菌根菌を共生させ、次いでVA菌
根菌が感染した植物を浄水ケーキからなる植物育成培土
で栽培することにより、VA菌根菌を効率良く増殖させ
ることを特徴とするVA菌根菌の増殖方法に関する。
The present invention relates to a plant-cultivating soil for mixing purified water cake with VA mycorrhizal fungi. Furthermore, the present invention cultivates a plant in which VA mycorrhizal fungi can co-exist in a plant cultivation medium in which purified water cake is mixed with VA mycorrhizal fungus,
The present invention relates to a method for growing a plant, which comprises cultivating the plant by allowing the mycorrhizal fungus to coexist with the plant to efficiently grow the VA mycorrhizal fungus. Furthermore, the present invention inoculates the VA mycorrhizal fungus into a plant growing soil consisting of a purified water cake, cultivates a plant in which the VA mycorrhizal fungus can co-exist, and causes the VA mycorrhizal fungus to coexist with the plant to generate the VA mycorrhizal fungus. The present invention relates to a plant growing method characterized by efficiently growing and cultivating a plant. Furthermore, the present invention provides a VA mycorrhizal fungus by symbioticly planting the VA mycorrhizal fungus in a plant, and then cultivating the plant infected with the VA mycorrhizal fungus in a plant growing soil consisting of a clean water cake. The present invention relates to a method for growing VA mycorrhizal fungi, which efficiently grows

【0007】[0007]

【発明の実施の形態】本発明に用いる浄水ケーキは浄水
処理過程で発生する沈積泥土を濃縮脱水したものであ
る。浄水ケーキは処理過程で硫酸や石灰または高分子凝
集剤等の薬剤を注入する薬注処理と薬剤を加えず濃縮あ
るいは濃縮・凍結融解後脱水処理を行う無薬注方式があ
り、本発明における植物育成培土として利用するために
は無薬注処理法により処理された浄水ケーキを用いるの
が望ましい。浄水ケーキを製造する工程で、河川水中の
浮遊物質を凝集させるために用いる凝集剤はポリ塩化ア
ルミニウムでも液体硫酸アルミニウムでもよい。また、
脱水方法は自然濃縮処理(天日乾燥)、加圧脱水、凍結
融解処理等のいずれでもよい。本発明に用いる浄水ケー
キの性質は、リン酸吸収係数2000mg/100g以
上、有効態リン酸10mg/100g以下が望ましい。
更には、全窒素0.3%以上、全リン酸0.1〜2.0
%が望ましい。このように、土壌に吸着固定され植物が
利用できないリン酸の量を示すリン酸吸収係数が高く、
且つ植物が利用できる土壌中のリン酸の量を示す有効態
リン酸が低い、浄水ケーキとVA菌根菌とを組合わせて
用いることによって、VA菌根菌の増殖が効率良く行な
われ、そのため植物の生育が良好となる、という優れた
効果を奏する。
BEST MODE FOR CARRYING OUT THE INVENTION The water purification cake used in the present invention is obtained by concentrating and dewatering sedimented mud generated in a water purification process. The purified water cake has a chemical injection treatment in which a chemical such as sulfuric acid or lime or a polymer flocculant is injected in the treatment process, and a non-chemical injection system in which the chemical is concentrated or concentrated / freeze-thaw and then dehydrated. In order to use it as cultivation soil, it is desirable to use purified water cake treated by the chemical-free treatment method. The flocculant used for flocculating the suspended solids in the river water in the step of producing the purified water cake may be polyaluminum chloride or liquid aluminum sulfate. Also,
The dehydration method may be any of natural concentration treatment (sun drying), pressure dehydration, freeze-thaw treatment and the like. Regarding the properties of the purified water cake used in the present invention, it is desirable that the phosphoric acid absorption coefficient is 2000 mg / 100 g or more and the effective phosphoric acid is 10 mg / 100 g or less.
Furthermore, total nitrogen 0.3% or more, total phosphoric acid 0.1-2.0
% Is desirable. In this way, the phosphate absorption coefficient, which indicates the amount of phosphoric acid that is adsorbed and fixed on the soil and cannot be used by plants, is high
And, by using a combination of the purified water cake and VA mycorrhizal fungus, which has a low effective phosphoric acid which indicates the amount of phosphoric acid in the soil that can be utilized by plants, the VA mycorrhizal fungi can be efficiently propagated. It has an excellent effect that the plant grows well.

【0008】本発明において浄水ケーキは発生したまま
の形態でもよいが、好ましくは直径10mm以下に解砕
し、調粒したものが適する。また、乾燥、造粒したもの
でもよい。また、VA菌根菌は、雑菌などによって死滅
しやすいので、浄水ケーキは、塩素殺菌やオゾン殺菌等
の消毒を受けた浄水ケーキを用いるのが好ましい。本発
明において植物育成培土として用いる浄水ケーキは、そ
れ単独でもよいが、施工の方法にあわせて堆肥化資材、
土壌改良材を混合してもよい。植栽用の土壌として用い
る場合、混合する堆肥化資材は通常知られているいずれ
の堆肥でよく、イナワラ、麦稈、バーク、鋸屑、家畜糞
尿、都市ゴミコンボスト等の各種材料を自然堆積しある
いは人為的に機械処理して、常法により得られる堆肥で
よい。これらの堆肥化資材の混合量は通常、浄水ケーキ
に対して5〜50%容量であり、好ましくは20〜30
%容量である。浄水ケーキに混合する土壌改良材は、ピ
ートモス、泥炭、木炭等の植物質資材でも、ゼオライ
ト、パーミキュライト、パーライトなどの鉱物質資材で
もよい。これらの土壌改良材の混合量は通常、浄水ケー
キに対して5〜50%容量であり、好ましくは10〜2
5%容量である。本発明の植物育成培土を法面緑化用の
生育基盤材として用いる場合、施工の方法にあわせて浄
水ケーキ、バーク堆肥、ピートモス等を適量混合すれば
よい。浄水ケーキの混合量は通常、生育基盤材中10〜
50%容量であり、好ましくは15〜30%容量であ
る。また、本発明では、浄水ケーキにリン酸肥料として
リンスター、カリ肥料として硫酸カリ、窒素肥料として
2−オキソ−4−メチル−6−ウレイドヘキサヒドロピ
リミジンを添加した、特開平5−219832号公報に
記載の育苗培土、あるいは、マンガンを過剰に含む浄水
ケーキにリン酸、またはリン酸と多孔質無機資材を添加
した、特開平7−227144号公報に記載の育苗培土
を用いてもよい。
In the present invention, the purified water cake may be in a form as it is generated, but it is preferably crushed into particles having a diameter of 10 mm or less and sized. Further, it may be dried and granulated. Further, since VA mycorrhizal fungi are easily killed by various bacteria, it is preferable to use a water purification cake that has been sterilized by chlorine sterilization, ozone sterilization, or the like. The water-purifying cake used as the plant growing soil in the present invention may be a single cake, but it is a composting material according to the method of construction,
Soil conditioners may be mixed. When used as soil for planting, the composting material to be mixed may be any of the commonly known composts, and various materials such as rice straw, wheat culm, bark, sawdust, livestock excrement, and municipal waste combo may be deposited naturally or artificially. It may be compost obtained by a conventional method after mechanical treatment. The mixing amount of these composting materials is usually 5 to 50% by volume with respect to the purified water cake, preferably 20 to 30.
% Capacity. The soil improving material to be mixed with the purified water cake may be a plant material such as peat moss, peat or charcoal, or a mineral material such as zeolite, permiculite or perlite. The amount of these soil conditioners mixed is usually 5 to 50% by volume, preferably 10 to 2 with respect to the purified water cake.
It is 5% capacity. When the plant growing soil of the present invention is used as a growth base material for slope greening, an appropriate amount of water-purifying cake, bark compost, peat moss, etc. may be mixed according to the construction method. The amount of purified water cake mixed is usually 10 to 10 in the growth base material.
It is 50% capacity, preferably 15-30% capacity. In addition, in the present invention, Japanese Patent Laid-Open No. 5-219832 discloses that purified water cake is added with phosphorus star as phosphate fertilizer, potassium sulfate as potassium fertilizer, and 2-oxo-4-methyl-6-ureidohexahydropyrimidine as nitrogen fertilizer. The seedling-raising soil described above, or the seedling-raising soil described in JP-A-7-227144 in which phosphoric acid or phosphoric acid and a porous inorganic material are added to a purified water cake containing excess manganese may be used.

【0009】本発明で用いるVA菌根菌としては、具体
的には、スカテロスポラ(Scutellospor
a)属、グロムス(Glomus)属、ギガスポラ(G
igaspora)属、アカウロスポラ(Acaulo
spora)属、スクレロシスティス(Scleroc
ystis)属、エントロフォスポラ(Entroph
ospora)属等に属するVA菌根菌を例示すること
ができる。これらのVA菌根菌の中でもギガスポラ(G
igaspora)属に属するVA菌根菌が適してい
る。特に、ギガスポラ属のマルガリータ(Margar
ita)種に属するVA菌根菌が好ましい。かかるVA
菌根菌は、有効態リン酸が低い土壌中で良好に増殖する
菌であるため、浄水ケーキと組合わせて用いるのに特に
適している。これらのVA菌根菌は天然界から収集する
ほか、栄養薄膜培養法(特開昭55−118390号公
報)や器官培養した根を使用する方法(特公昭62−4
9037号公報)等により培養したものでもよく、ま
た、一般に市販されているものでもよい。また、浄水ケ
ーキを用いてVA菌根菌を培養したものを用いてもよ
い。
Specific examples of the VA mycorrhizal fungi used in the present invention include Scutellospora.
a) Genus, Glomus genus, Gigaspora (G)
genus igaspora, Acaulospo
spora), Sclerocystis (Scleroc)
genus ystis, Entropophora
VA mycorrhizal fungi belonging to the genus Ospora) can be exemplified. Among these VA mycorrhizal fungi, gigaspora (G
VA mycorrhizal fungi belonging to the genus igaspora) are suitable. In particular, Margarita (Margarita of the genus Gigaspora)
VA) mycorrhizal fungi belonging to the species ita) are preferred. Such VA
Mycorrhizal fungi are particularly suitable for use in combination with clean water cakes because they are well grown in soil with low available phosphate. In addition to collecting these VA mycorrhizal fungi from the natural world, a nutrient thin film culture method (JP-A-55-118390) and a method using organ-cultured roots (Japanese Patent Publication No. 62-4).
9037) and the like, or those commercially available in general. Moreover, you may use what culture | cultivated VA mycorrhizal fungi using the purified water cake.

【0010】VA菌根菌の浄水ケーキへの添加量は、V
A菌根菌が植物に対して感染できる程度の量を用いれば
よいが、通常、本発明の植物育成培土1リットル中に胞
子が10個以上、好ましくは100個以上含まれる量
で、浄水ケーキに添加するのがよい。かくして得られ
る、浄水ケーキにVA菌根菌を混合してなる本発明の植
物育成培土で、植物を栽培することによって、VA菌根
菌が植物に共生して効率良く増殖し、植物に良好に生育
する。
The amount of VA mycorrhizal fungus added to the purified water cake is V
The amount of the mycorrhizal fungus to be able to infect plants may be used, but usually, 1 liter of the plant cultivation medium of the present invention contains 10 or more spores, preferably 100 or more spores. It is good to add to. By cultivating the plant with the plant growing medium of the present invention obtained by mixing the VA mycorrhizal fungus in the purified water cake thus obtained, the VA mycorrhizal fungus grows symbiotically with the plant and efficiently grows in the plant. To grow.

【0011】栽培の対象となる植物は、VA菌根菌が共
生しうる植物であり、ダイズ、クローバ、マングビー
ン、ピーナッツ、アルファルファ、ウマゴヤシ、カラス
ノエンドウ、ハギ等のマメ科植物、メヒシバ、スダング
ラス、バヒヤグラス、ギニアグラス、シバ等のイネ科植
物、シャリンバイ、コデマリ等のバラ科植物、ヤマツツ
ジ、オオムラサキツツジ等のツツジ科植物、イロハモミ
ジ等のカエデ科植物、サイネリア、ヨモギ等のキク科植
物、ベゴニア等のシュウカイドウ科植物等ほとんどの緑
化植栽用植物が含まれる。植物の栽培は、通常の方法を
採用することによって実施でき、特に制限されない。特
に、VA菌根菌の増殖を考慮すれば、例えば、温度は5
〜40℃、特に15〜30℃で栽培するのが好ましい。
The plants to be cultivated are plants which can coexist with VA mycorrhizal fungi, and include legumes such as soybean, clover, mung bean, peanut, alfalfa, horse mackerel, crow peas, and hagi, witch clover, Sudangrass, Bahiyagrass, guinea grass, grasses such as turf, Sharinbai, roses such as Kodemari, azaleas, azaleas such as Oomurasaki azalea, maple plants such as Japanese maple, asteraceae such as cineraria, mugwort, begonia etc. Includes almost all greening planting plants, such as Gypsophilidae. Cultivation of the plant can be carried out by adopting an ordinary method and is not particularly limited. In particular, if the growth of VA mycorrhizal fungi is taken into consideration, for example, the temperature is 5
It is preferable to cultivate at -40 ° C, especially at 15-30 ° C.

【0012】また、本発明では、浄水ケーキにあらかじ
めVA菌根菌を添加した植物育成培土を用いる代わり
に、浄水ケーキからなる植物育成培土を直接施用し、そ
こにVA菌根菌を接種して、植物を栽培することもでき
る。この場合に用いる浄水ケーキからなる植物育成培土
は、前記したように、浄水ケーキ単独でもよく、堆肥化
資材、土壌改良材などを混合したものを用いることがで
きる。VA菌根菌は、培土に植物を播種または移植する
際に、同時に種菌を施用することができる。VA菌根菌
の接種量は、植物に感染できる程度の量で十分である。
Further, in the present invention, instead of using the plant growing medium containing the VA mycorrhizal fungus in advance in the purified water cake, the plant growing medium consisting of the purified water cake is directly applied and inoculated with the VA mycorrhizal fungus. , It is also possible to grow plants. As described above, the plant-cultivating soil consisting of the purified water cake used in this case may be the purified water cake alone or may be a mixture of the composting material, the soil improving material and the like. The VA mycorrhizal fungus can be applied at the same time when the plant is sown or transplanted to the soil. The inoculation amount of VA mycorrhizal fungi is sufficient to infect plants.

【0013】また、本発明では、VA菌根菌が共生しう
る植物にVA菌根菌を共生させ、次いで、VA菌根菌が
感染した植物を浄水ケーキからなる植物育成培土で栽培
することによって、VA菌根菌を効率良く増殖させるこ
とができる。このような増殖方法において、VA菌根菌
を植物に感染させる方法としては、特に制限はなく、ま
た施行時期としても、該当植物の発根前、発根後のいず
れであってもよい。特に、播種、挿し木、挿し芽の前処
理等、播種、挿し木、挿し芽と同時期、または苗の定植
時等が好ましい。また、施行方法としては、土壌と混
合、株元への混入、土壌下層での積層、植え穴への混入
等が好ましい。VA菌根菌の宿主となる植物の栽培環境
は、既存法に従えばよく、例えば、温度は5〜40℃、
特に15〜30℃が好ましい。
Further, in the present invention, a plant capable of coexisting with VA mycorrhizal fungus is allowed to coexist with VA mycorrhizal fungus, and then a plant infected with VA mycorrhizal fungus is cultivated in a plant growing medium comprising a clean water cake. , VA mycorrhizal fungi can be efficiently propagated. In such a growth method, the method of infecting a plant with VA mycorrhizal fungi is not particularly limited, and the time of execution may be either before or after rooting of the corresponding plant. In particular, seeding, cutting, pretreatment of cuttings, etc., seeding, cutting, the same time as cutting, or at the time of planting seedlings is preferable. In addition, as an implementation method, it is preferable to mix with soil, mix into the root of the plant, stack under the soil, mix into planting holes, and the like. The environment for cultivating the plant that becomes the host of VA mycorrhizal fungi may be in accordance with the existing method, for example, the temperature is 5 to 40 ° C.,
Particularly, 15 to 30 ° C. is preferable.

【0014】本発明におけるVA菌根菌の増殖の場とし
て天日乾燥床を利用できる。天日乾燥床は、浄水ケーキ
である濃縮された沈殿汚泥をマスの中に溜め、天日によ
って乾燥させる施設で、一定の含水率(60%以下)に
まで乾燥させるのに夏期1.5〜2ヶ月、冬期3〜6ヶ
月必要なため、その間に宿主作物を栽培し、VA菌根菌
を培養できる。より具体的には以下の方法を採用するこ
とができる。即ち、天日乾燥床に溜められた沈殿汚泥の
含水率が80%以下になったら耕耘し、宿主植物を播種
または移植する。宿主植物としてはマメ科植物のクロー
バー、ウマゴヤシ、ハギ、イネ科植物のアルファルフ
ァ、カラスノエンドウを単植、または数種類を混植す
る。宿主作物の栽培開始時にVA菌根菌(好ましくはG
igaspora属、Glomus属)を接種する。施
肥は無施肥でよいが、栽培初期にリン酸肥料を成分で
0.01〜1.0g/土1リットル添加しても良い。栽
培は3〜4月に開始し、8〜9月に完了する。ビニール
フィルム等による被覆を行ったり、加温することによっ
て栽培開始を早めることができる。灌水は自然降雨を利
用するが、降雨が少ない場合は適宜灌水を行う。
A sun dried bed can be used as a place for the growth of VA mycorrhizal fungi in the present invention. The sun drying bed is a facility that collects the concentrated settled sludge, which is a purified water cake, in a trout and dries it in the sun. It is possible to dry it to a certain water content (60% or less) in the summer 1.5 ~. Since 2 months and 3 to 6 months in winter are required, a host crop can be cultivated during that period and VA mycorrhizal fungi can be cultivated. More specifically, the following method can be adopted. That is, when the water content of the settled sludge stored in the sun drying bed becomes 80% or less, it is cultivated and the host plant is sown or transplanted. As the host plants, clover, horse mackerel, and barley of the legumes, alfalfa of the grasses, and oak peas are planted alone or several types are mixed. At the start of cultivation of the host crop, VA mycorrhizal fungus (preferably G
(Igaspora, Glomus). Fertilization may be applied without fertilization, but phosphate fertilizer may be added as an ingredient in an amount of 0.01 to 1.0 g / soil 1 liter at the initial stage of cultivation. Cultivation starts in March to April and is completed in August to September. The cultivation can be started earlier by covering with a vinyl film or by heating. Although natural rainfall is used for irrigation, irrigation is performed as appropriate when rainfall is low.

【0015】VA菌根菌の培養は、温室内に以下のよう
にして実施することもできる。浄水ケーキを0.5〜1
リットル容量のポットに詰め、宿主植物を播種または移
植する。宿主植物としてはマメ科植物のクローバー、ウ
マゴヤシ、ハギ、イネ科植物のアルファルファ、カラス
ノエンドウを用いる。宿主作物の栽培開始時にVA菌根
菌(好ましくは、Gigaspora属、Glomus
属)を接種する。施肥は無施肥でよいが、栽培初期にリ
ン酸肥料を成分で0.01〜1.0g/土1リットル添
加しても良い。気温を10〜40℃、好ましくは20〜
30℃で管理し、適宜灌水を行う。栽培を開始し、2ヶ
月以上経過後に完了する。
Cultivation of VA mycorrhizal fungi can also be carried out in a greenhouse as follows. Clean water cake 0.5-1
It is packed in a liter capacity pot and seeded or transplanted with a host plant. As a host plant, legumes such as clover, horse mackerel, and hagi, gramineous plants such as alfalfa and corngrass are used. At the start of cultivation of the host crop, VA mycorrhizal fungi (preferably Gigaspora genus, Glomus
(Genus). Fertilization may be applied without fertilization, but phosphate fertilizer may be added as an ingredient in an amount of 0.01 to 1.0 g / soil 1 liter at the initial stage of cultivation. Temperature is 10 to 40 ° C, preferably 20 to
It is maintained at 30 ° C. and watered appropriately. Cultivation is started and completed after 2 months or more.

【0016】上記したVA菌根菌の増殖法においては、
VA菌根菌は植物に対し感染できる程度の量を用いれば
よい。特に大量の土を用いて増殖する場合は宿主植物の
播種時にVA菌根菌の種菌を同時に施用するのがよい。
宿主植物の生育とともにVA菌根菌の増殖も旺盛にな
り、この増殖したVA菌根菌が付着した基材は、そのま
ま種菌としても使用できる。通常、2ヶ月以上栽培する
と胞子を形成し始める。より多くの胞子を形成させる場
合は宿主作物が十分に生育したところで、灌水などを絶
ちしばらく放置する。この栽培土壌から湿式篩別法(鈴
木達彦著、VA菌根に対する諸問題5,農業及び園芸、
第62巻、第3号、P.28、1987年)等により胞
子を分離、回収し、必要に応じて増量剤などを加えれば
VA菌根菌製剤として用いることもできる。
In the above-mentioned method for growing VA mycorrhizal fungi,
The VA mycorrhizal fungi may be used in an amount enough to infect plants. Especially when growing a large amount of soil, it is advisable to apply the inoculum of VA mycorrhizal fungi at the same time when sowing the host plant.
Propagation of VA mycorrhizal fungi becomes vigorous as the host plant grows, and the substrate to which the proliferated VA mycorrhizal fungi adheres can be used as it is as an inoculum. Usually, when cultivated for 2 months or more, it starts to form spores. When more spores are to be formed, irrigation is stopped and left for a while when the host crop has grown sufficiently. Wet sieving method from this cultivated soil (Tatsuhiko Suzuki, Problems on VA mycorrhiza 5, Agriculture and Horticulture,
Vol. 62, No. 3, P. 28, 1987) and the like, and the spores are separated and collected, and if necessary, a bulking agent and the like may be added to be used as a VA mycorrhizal preparation.

【0017】[0017]

【発明の効果】以上に詳述した通り、浄水ケーキにVA
菌根菌を混合してなる植物育成培土で、VA菌根菌が共
生できる植物を栽培することによって、植物を良好に生
育させることが可能となる。また、VA菌根菌が感染し
た植物を浄水ケーキからなる植物育成培土で栽培するこ
とにより、VA菌根菌を効率良く増殖させることができ
る。
As described above in detail, VA is added to the purified water cake.
By cultivating a plant capable of coexisting with VA mycorrhizal fungus in a plant growing soil containing a mixture of mycorrhizal fungi, it becomes possible to grow the plant well. In addition, VA mycorrhizal fungi can be efficiently propagated by cultivating a plant infected with VA mycorrhizal fungi in a plant growing soil consisting of a purified water cake.

【0018】[0018]

【実施例】以下、本発明を実施例により、更に詳細に説
明する。 実施例1 供試培土として、5m/mのふるいにかけた浄水ケーキ
(リン酸吸収係数2300〜2500mg/100g;
有機態リン酸3〜5mg/100g;全窒素0.8〜
1.2%;全リン酸0.1〜2.0%)にバーク堆肥を
20%容量混合し(有効態リン酸は10〜20mg/1
00gとなった)、さらに茨城県つくば市より採取した
土壌から湿式篩別法で集めたVA菌根菌Gigaspo
ra Margaritaの胞子10個/土1リットル
を混合したものを用いた。対照として茨城県つくば市よ
り採取した畑土にバーク堆肥を20%混合し、IB化成
肥料(N−P−K=10−10−10)を、0.5g/
土1リットル添加したものを用いた。10リットル容量
のポットに供試培土及び畑土をそれぞれ詰め、1995
年4月にコノテガシワ、イロハモミジ、オオムラサキツ
ツジの苗を移植、ノシバを播種した。露地にて必要に応
じて灌水を行いながら栽培し、6ヶ月後の樹高または刈
り芝重量を測定した。結果を第1表に示す。
The present invention will be described in more detail with reference to the following examples. Example 1 As a test soil, a purified water cake (phosphoric acid absorption coefficient 2300 to 2500 mg / 100 g; sieved through a 5 m / m sieve;
Organic phosphoric acid 3-5 mg / 100 g; total nitrogen 0.8-
1.2%; 0.1% to 2.0% total phosphoric acid) mixed with 20% by volume of bark compost (10 to 20 mg / 1 of effective phosphoric acid)
00g), and VA mycorrhizal fungus Gigaspo collected from soil collected from Tsukuba City, Ibaraki Prefecture by wet sieving method.
A mixture of 10 spores of Ra Margarita / l liter of soil was used. As a control, 20% of bark compost was mixed with upland soil collected from Tsukuba City, Ibaraki Prefecture, and IB chemical fertilizer (NPK = 10-10-10) was added at 0.5 g /
The one to which 1 liter of soil was added was used. Fill the 10 liter capacity pot with the test soil and the field soil, 1995
In April 2013, seedlings of Konotegashiwa, Acer palmatum, and Omurasaki azalea were transplanted and sowed. Cultivation was performed in the open field with watering if necessary, and after 6 months, the tree height or the cut grass weight was measured. The results are shown in Table 1.

【0019】[0019]

【表1】 第1表 ─────────────────────────────────── 供試培土 樹高伸長量 (cm) 刈り芝重量(g) コノテカ゛シワ イロハモミシ゛ オオムラサキツツシ゛ ノシバ ─────────────────────────────────── 浄水ケーキ 15.0 89.0 18.0 626 畑土 13.0 43.3 12.7 348 ───────────────────────────────────[Table 1] Table 1 ─────────────────────────────────── Test cultivated soil Tree height growth (cm ) Weight of mowed grass (g) Konoteka iwairohamomiji oomurasakitsutsuji noshiba ─────────────────────────────────── Clean water cake 15.0 89.0 18.0 626 Upland 13.0 43.3 12.7 348 ────────────────────────────────────

【0020】第1表の結果から明らかなように、浄水ケ
ーキにVA菌根菌を混合した培土で植物を栽培すること
によって、良好に植物が生育できる。
As is clear from the results shown in Table 1, the plants can be satisfactorily grown by cultivating the plants in a soil containing VA mycorrhizal fungi in a purified water cake.

【0021】実施例2 供試培土として5m/mのふるいにかけた実施例1と同
様の特性を有する浄水ケーキにバーク堆肥を20%容量
混合し、さらに茨城県つくば市より採取した土壌から湿
式篩別法で集めたVA菌根菌Gigaspora sp
の胞子20個/土1リットルを混合したものを用いた。
対照として茨城県つくば市より採取した畑土にバーク堆
肥を20%混合し、IB化成肥料(N−P−K=10−
10−10)を、0.5g/土1リットル添加したもの
を用いた。10リットル容量のポットに供試培土及び畑
土をそれぞれ詰め、1995年4月にコノテガシワ、イ
ロハモミジ、オオムラサキツツジの苗を移植、ノシバを
播種した。露地にて必要に応じて灌水を行いながら栽培
し、6ヶ月後の樹高または刈り芝重量を測定した。結果
を第2表に示す。
Example 2 20% by volume of bark compost was mixed with purified water cake having the same characteristics as in Example 1 which had been sifted through a 5 m / m sieve as a test soil, and the wet sieve was obtained from soil collected from Tsukuba City, Ibaraki Prefecture. VA mycorrhizal fungus Gigaspora sp collected by another method
A mixture of 20 spores per liter of soil was used.
As a control, 20% of bark compost was mixed with upland soil collected from Tsukuba City, Ibaraki Prefecture, and IB chemical fertilizer (NPK = 10-
10-10) added with 0.5 g / soil 1 liter was used. A 10-liter capacity pot was filled with each of the test soil and field soil, and in April 1995, seedlings of Konotega wrinkle, Acer palmatum, and Omurasaki azalea were transplanted and sowed. Cultivation was performed in the open field with watering if necessary, and after 6 months, the tree height or the cut grass weight was measured. The results are shown in Table 2.

【0022】[0022]

【表2】 第2表 ─────────────────────────────────── 供試培土 樹高伸長量 (cm) 刈り芝重量(g) コノテカ゛シワ イロハモミシ゛ オオムラサキツツシ゛ ノシバ ─────────────────────────────────── 浄水ケーキ 16.2 85.3 17.8 721 畑土 13.0 43.3 12.7 348 ───────────────────────────────────[Table 2] Table 2 ─────────────────────────────────── Test cultivated soil Tree height growth (cm ) Weight of mowed grass (g) Konoteka iwairohamomiji Oomurasaki Tsutsuji Noshiba ──────────────────────────────────── Clean water cake 16.2 85.3 17.8 721 Upland 13.0 43.3 12.7 348 ────────────────────────────────────

【0023】第2表の結果から明らかなように、浄水ケ
ーキにVA菌根菌を混合した培土で植物を栽培すること
により、良好に植物が生育できる。 実施例3 供試培土として5m/mのふるいにかけた実施例1と同
様の特性を有する浄水ケーキと、芝の目土(粒径1〜2
mm;リン酸吸収係数約1500mg/100g;有効
態リン酸0〜5mg/100g;全窒素0〜0.1%;
全リン酸0〜0.2%)にそれぞれリン酸肥料をリン酸
成分で0.2g/土1リットル添加したものを用いた。
1リットル容量のポットに供試培土を詰め、ホワイトク
ローバーを播種した。茨城県つくば市より採取した土壌
から湿式篩別法で集めたVA菌根菌Gigaspora
Margaritaの胞子10個を、播種の際に各供
試培土に接種した。気温15〜30度になるように調節
したガラス温室内で、適宜灌水しながら植物を栽培し、
3ヶ月経過後、灌水を中止し2週間放置した。その後、
供試培土中に含まれるGigaspora Marga
ritaの胞子数を測定した。結果を第3表に示す。
As is clear from the results shown in Table 2, the plant can be satisfactorily grown by cultivating the plant in the soil containing the VA mycorrhizal fungus in the purified water cake. Example 3 A water-purified cake having the same characteristics as in Example 1 which was passed through a 5 m / m sieve as a test soil, and a grass soil (particle size 1 to 2).
mm; phosphoric acid absorption coefficient of about 1500 mg / 100 g; effective phosphoric acid 0 to 5 mg / 100 g; total nitrogen 0 to 0.1%;
A phosphoric acid fertilizer was added to each of the phosphoric acid fertilizers in an amount of 0.2 g per liter of soil to 0% to 0.2% of total phosphoric acid).
A 1-liter capacity pot was filled with the test soil and seeded with white clover. VA mycorrhizal fungus Gigaspora collected by wet sieving method from soil collected from Tsukuba City, Ibaraki Prefecture
Ten Margarita spores were inoculated into each test soil during sowing. Cultivate plants while appropriately irrigating them in a glass greenhouse adjusted to an air temperature of 15 to 30 degrees Celsius,
After 3 months, irrigation was stopped and left for 2 weeks. afterwards,
Gigaspora Marga contained in test soil
The number of spores of rita was measured. The results are shown in Table 3.

【0024】[0024]

【表3】 第3表 ─────────────────────────────────── 供試培土 Gigaspora Margarita 胞子数(個/土1ml) ─────────────────────────────────── 浄水ケーキ 6.67 芝の目土 2.50 ───────────────────────────────────[Table 3] Table 3 ─────────────────────────────────── Test soil Soil Gigaspora Margarita Number of spores ( 1 piece / soil 1 ml) ─────────────────────────────────── Water purification cake 6.67 Grass soil 2 .50 ───────────────────────────────────

【0025】第3表の結果から、浄水ケーキからなる培
土でVA菌根菌を感染させた植物を栽培することによ
り、VA菌根菌を効率良く増殖できることが判る。 実施例4 供試培土として5m/mのふるいにかけた実施例3と同
様の特性を有する浄水ケーキと芝の目土(粒径1〜2m
m)にそれぞれリン酸肥料をリン酸成分で0.2g/土
1リットル添加したものを用いた。1リットル容量のポ
ットに供試培土を詰め、ホワイトクローバーを播種し
た。茨城県つくば市より採取した土壌から湿式篩別法で
集めたVA菌根菌Gigaspora spの胞子10
個を、播種の際に各供試培土に接種した。気温15〜3
0度になるように調節したガラス温室内で、適宜灌水し
ながら植物を栽培し、3ヶ月経過後、灌水を中止し2週
間放置した。その後、供試培土中に含まれるGigas
pora spの胞子数を測定した。結果を第4表に示
す。
From the results shown in Table 3, it can be seen that VA mycorrhizal fungi can be efficiently grown by cultivating a plant infected with VA mycorrhizal fungi in a soil consisting of a purified water cake. Example 4 A purified water cake having the same characteristics as those of Example 3, which was passed through a 5 m / m sieve as a test soil, and a grass soil (particle size: 1 to 2 m).
The phosphoric acid fertilizer added with 0.2 g of phosphoric acid component / 1 liter of soil was used for m). A 1-liter capacity pot was filled with the test soil and seeded with white clover. Spores 10 of VA mycorrhizal fungus Gigaspora sp collected by wet sieving from soil collected from Tsukuba City, Ibaraki Prefecture
Each seed medium was inoculated at the time of sowing. Temperature 15 ~ 3
The plant was cultivated in a glass greenhouse adjusted to 0 degree with appropriate watering, and after 3 months, watering was stopped and left for 2 weeks. After that, Gigas contained in the test soil
The number of spores of Pora sp was measured. The results are shown in Table 4.

【0026】[0026]

【表4】 第4表 ─────────────────────────────────── 供試培土 Gigaspora sp胞子数(個/土1ml) ─────────────────────────────────── 浄水ケーキ 35.5 芝の目土 15.3 ───────────────────────────────────[Table 4] Table 4 ─────────────────────────────────── Test soil Soil Gigaspora sp Number of spores ( Pieces / soil 1ml) ─────────────────────────────────── Purified water cake 35.5 Turf stains 15 .3 ───────────────────────────────────

【0027】第4表の結果から、浄水ケーキからなる培
土でVA菌根菌を感染させた植物を栽培することによ
り、VA菌根菌を効率良く増殖できることが判る。 実施例5 供試培土として5m/mのふるいにかけた実施例3と同
様の特性を有する浄水ケーキと芝の目土(粒径1〜2m
m)にそれぞれリン酸肥料をリン酸成分で0.2g/土
1リットル添加したものを用いた。10リットル容量の
ポットに供試培土を詰め、ファルカタ(マメ科樹木)A
lbizia Falcataria(L.) Fos
bを播種した。茨城県つくば市より採取した土壌から湿
式篩別法で集めたVA菌根菌Gigaspora Ma
rgaritaの胞子10個を、播種の際に各供試培土
に接種した。気温15〜30度になるように調節したガ
ラス温室内で、適宜灌水しながら植物を栽培し、3ヶ月
経過後、灌水を中止し2週間放置した。その後、供試培
土中に含まれるGigaspora Margarit
aの胞子数を測定した。結果を第5表に示す。
From the results shown in Table 4, it is understood that VA mycorrhizal fungi can be efficiently propagated by cultivating a plant infected with VA mycorrhizal fungi in a soil consisting of purified water cake. Example 5 A purified water cake having the same characteristics as those of Example 3, which was passed through a 5 m / m sieve as a test soil, and grass soil (particle size: 1 to 2 m).
The phosphoric acid fertilizer added with 0.2 g of phosphoric acid component / 1 liter of soil was used for m). Falcata (leguminous tree) A filled with test soil in a 10-liter capacity pot
lbizia Falcataria (L.) Fos
b was seeded. VA mycorrhizal fungus Gigaspora Ma collected by wet sieving method from soil collected from Tsukuba City, Ibaraki Prefecture
10 spores of rgarita were inoculated into each test soil during sowing. A plant was cultivated while appropriately irrigating in a glass greenhouse controlled to have an air temperature of 15 to 30 degrees, and after 3 months, irrigation was stopped and left for 2 weeks. After that, Gigaspora Margarit contained in the test soil
The spore number of a was measured. The results are shown in Table 5.

【0028】[0028]

【表5】 第5表 ─────────────────────────────────── 供試培土 Gigaspora Margarita 胞子数(個/土1ml) ─────────────────────────────────── 浄水ケーキ 4.00 芝の目土 1.10 ───────────────────────────────────[Table 5] Table 5 ─────────────────────────────────── Test soil Soil Gigaspora Margarita Number of spores ( 1 piece / soil 1ml) ─────────────────────────────────── Purified water cake 4.00 Grass soil 1 .10 ───────────────────────────────────

【0029】第5表の結果から、浄水ケーキからなる培
土でVA菌根菌を感染させた植物を栽培することによ
り、VA菌根菌を効率良く増殖できることが判る。
From the results shown in Table 5, it can be seen that VA mycorrhizal fungi can be efficiently propagated by cultivating a plant infected with VA mycorrhizal fungi in a soil consisting of purified water cake.

─────────────────────────────────────────────────────
─────────────────────────────────────────────────── ───

【手続補正書】[Procedure amendment]

【提出日】平成9年2月14日[Submission date] February 14, 1997

【手続補正1】[Procedure amendment 1]

【補正対象書類名】明細書[Document name to be amended] Statement

【補正対象項目名】特許請求の範囲[Correction target item name] Claims

【補正方法】変更[Correction method] Change

【補正内容】[Correction contents]

【特許請求の範囲】[Claims]

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 FI 技術表示箇所 C12N 1/14 C12N 1/14 B // C09K 101:00 ─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 6 Identification code Internal reference number FI technical display location C12N 1/14 C12N 1/14 B // C09K 101: 00

Claims (7)

【特許請求の範囲】[Claims] 【請求項1】 浄水ケーキにVA菌根菌を混合してなる
植物育成培土。
1. A plant growing soil obtained by mixing a VA mycorrhizal fungus with a purified water cake.
【請求項2】 浄水ケーキが、リン酸吸収係数が200
0mg/100g以下であり且つ有効態リン酸が10m
g/100g以下である請求項1記載の植物育成培土。
2. The purified water cake has a phosphoric acid absorption coefficient of 200.
0 mg / 100 g or less and effective phosphoric acid is 10 m
The plant cultivation soil according to claim 1, which is g / 100 g or less.
【請求項3】 VA菌根菌がギガスポラ属に属する請求
項1記載の植物育成培土。
3. The plant cultivation medium according to claim 1, wherein the VA mycorrhizal fungus belongs to the genus Gigaspora.
【請求項4】 植物育成培土1リットル中にVA菌根菌
の胞子を10個以上混合してなる請求項1記載の植物育
成培土。
4. The plant growing soil according to claim 1, wherein 10 liters or more of VA mycorrhizal fungi are mixed in 1 liter of the plant growing soil.
【請求項5】 浄水ケーキにVA菌根菌を混合してなる
植物育成培土でVA菌根菌が共生しうる植物を栽培し、
VA菌根菌を植物に共生させてVA菌根菌を効率よく増
殖させて、植物を栽培することを特徴とする植物育成方
法。
5. A plant capable of coexisting with VA mycorrhizal fungus is cultivated in a plant growing soil obtained by mixing VA mycorrhizal fungus in a purified water cake.
A method for growing a plant, which comprises cultivating a plant by allowing the VA mycorrhizal fungus to coexist with the plant to efficiently grow the VA mycorrhizal fungus.
【請求項6】 浄水ケーキからなる植物育成培土にVA
菌根菌を接種して、VA菌根菌が共生しうる植物を栽培
し、VA菌根菌を植物に共生させてVA菌根菌を効率よ
く増殖させて、植物を栽培することを特徴とする植物育
成方法。
6. A VA for plant growing soil made of purified water cake
Inoculating the mycorrhizal fungus, cultivating a plant in which the VA mycorrhizal symbiosis is possible, allowing the VA mycorrhizal fungus to symbiosis with the plant to efficiently grow the VA mycorrhizal fungus, and culturing the plant. How to grow plants.
【請求項7】 VA菌根菌が共生しうる植物にVA菌根
菌を共生させ、次いでVA菌根菌が感染した植物を浄水
ケーキからなる植物育成培土で栽培することにより、V
A菌根菌を効率よく増殖させることを特徴とするVA菌
根菌の増殖方法。
7. A plant capable of coexisting with VA mycorrhizal fungi is allowed to coexist with VA mycorrhizal fungi, and then a plant infected with VA mycorrhizal fungus is cultivated in a plant growing medium consisting of a water-purified cake to obtain V.
A method for growing VA mycorrhizal fungi, which comprises efficiently growing A mycorrhizal fungi.
JP6313196A 1996-03-19 1996-03-19 Plant cultivation soil Expired - Fee Related JP3136265B2 (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000139113A (en) * 1998-11-11 2000-05-23 Sumitomo Forestry Co Ltd Afforestation of deserted land
JP2002058334A (en) * 2000-08-18 2002-02-26 Okutama Kogyo Co Ltd Culture soil utilizing sludge
KR100852770B1 (en) * 2007-05-17 2008-08-18 충북대학교 산학협력단 Amf-inoculated bio-soilbeds useful in capsicum annuum cultivation and method for preparing the same
JP2013146255A (en) * 2012-01-23 2013-08-01 Mogami Ranen Kk Mass propagation method of chrysanthemum pacificum, greening method using chrysanthemum pacificum, and cultivation method of wood rotting fungus used therefor

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000139113A (en) * 1998-11-11 2000-05-23 Sumitomo Forestry Co Ltd Afforestation of deserted land
JP2002058334A (en) * 2000-08-18 2002-02-26 Okutama Kogyo Co Ltd Culture soil utilizing sludge
KR100852770B1 (en) * 2007-05-17 2008-08-18 충북대학교 산학협력단 Amf-inoculated bio-soilbeds useful in capsicum annuum cultivation and method for preparing the same
JP2013146255A (en) * 2012-01-23 2013-08-01 Mogami Ranen Kk Mass propagation method of chrysanthemum pacificum, greening method using chrysanthemum pacificum, and cultivation method of wood rotting fungus used therefor

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