JPH0920660A - Stress-resistant composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a stress-resistant composition expressing a reducing action for stresses during mental activities such as calculation, memorization, etc., and mental and physical stresses generated when one can not dissolve by its own ability and useful as medicines and functional foods by allowing to contain a specific imidazole compound. SOLUTION: This stress-resistant composition contains one or more imidazole compound selected from a group consisting of anserine, balenine, π- methylhistidine and τ-methylhistidine. Functional food is preferably beverage or food having a form capable of taking 50mg-5g imidazole per day. It is preferable to use e.g. an extract which has been extracted, separated and purified from a broth discharged in producing dried bonitos or small dried sardines.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition such as a medicine or a functional food containing anserine, valenin, π-methylhistidine or τ-methylhistidine as an active ingredient, and anserine, valenin, π-methylhistidine and τ. -To a food or drink substantially free of methylhistidine, by adding one or more imidazole compounds selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine or an extract containing the same as the main component, The present invention relates to a novel food and drink, and a method for producing the same.

[0002]

2. Description of the Related Art The control organs such as the central nervous system, endocrine system and immune system respond to changes and stimuli in the in vitro environment as much as possible.
It has the function of trying to maintain the homeostasis of the biological environment. However, when fluctuations and stimuli that exceed the limits of this regulatory function are applied, it becomes difficult to maintain homeostasis, and this is called stress. According to the research so far,
Under stress, it has been clarified that the neurohormonal balance is disrupted and endocrine abnormalities are caused by hyperexcitatory stimulation of the brainstem reticular formation. Therefore, stress has not only a psychological effect on a living body but also a tissue organ or physical effect.

Specifically, it is known that stress reduces the function of internal organs such as the liver and the ability to use energy sources in the body such as sugar. In recent years, the involvement of stress as a cause of various diseases has been suggested, and a growing social environment in which stress is increasing has become a major problem. Against this background, there is a strong demand for the development of a drug or food or drink that has a safe anti-stress effect that effectively prevents or reduces various disorders caused by stress.

On the other hand, anserine (β-alanyl-π-methyl-L-histidine) and varenin (β-alanyl-
(.tau.-methyl-L-histidine) is a dipeptide existing in muscle tissues of mammals, birds, reptiles, amphibians and the like, and is a known substance. Further, π-methylhistidine and τ-methylhistidine are known as biosynthetic intermediates of anserine and valenin, respectively. Many studies have been conducted since these dipeptides were discovered at the beginning of this century, and it has been reported that anserine and valenin are present in vertebrate skeletal muscle in a concentration range of 1-20 mM. Its content is known to change with the type of muscle and the age of the animal, but its physiological action has not been completely clarified yet.

However, in recent years, research on the action and effect has been carried out, and in JP-A-1-246218, it is disclosed that L-anserin has an immunoregulatory action.
Japanese Patent Publication No. 6166 discloses that carnosine, anserine and varenin have a blood pressure elevation suppressing effect.
In 299 publication, carnosine zinc salt prevents liver damage,
It is useful as a therapeutic agent, and the literature (Japanese Physiology (1990) 52, 221-228) discloses the liver function-protecting action of L-carnosine and the metabolism promoting action of stress-related substances.

Further, in Japanese Patent Application Laid-Open No. 4-187067, Japanese Patent Publication No. 63-47 discloses that carnosine, anserine and valenin have an antioxidant effect on protein foods.
Japanese Patent No. 435 discloses that carnosine, anserine or valenin is added so that the product content is 0.1% or more, and the pH is 6.5 or more to improve the taste of the product. There is. However, it has not been known at all that anserine, valenin, π-methylhistidine and τ-methylhistidine have anti-stress effects.

[0007]

Therefore, the present invention provides a highly safe composition such as an anti-stress agent or a functional food having an anti-stress action, as well as anserine, valenin, π-methylhistidine and τ-methylhistidine. To foods and beverages that do not substantially contain anserine, valenin, one or more imidazole compounds selected from the group of π-methylhistidine and τ-methylhistidine, or a novel addition of an extract containing this as a main component The present invention aims to provide a food and drink or a food and drink having an anti-stress action.

[0008]

Means for Solving the Problems As a result of earnest research to solve the above problems, the present inventors have found that anserine,
Valenin, π-methylhistidine, τ-methylhistidine are stress during mental activities such as calculation and memory,
They have found that they have an effect of alleviating mental stress, physical stress, etc. that occur when they cannot solve by their own abilities, and have completed the present invention. Therefore, the present invention is intended to provide an anti-stress composition comprising one or more imidazole compounds selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine.

The present invention also includes anserine, valenin, π
-Methylhistidine and τ-methylhistidine substantially in food and drink, anserine, varenin, one or more imidazole compounds selected from the group of π-methylhistidine and τ-methylhistidine, or containing it as a main component The present invention relates to a novel food or drink product obtained by adding an extract or a food or drink product having an anti-stress action. Furthermore, anserine, barrenin, food and drink that does not substantially contain π-methylhistidine and τ-methylhistidine, anserine, valenin, π-methylhistidine, and τ-methylhistidine or an extract containing this as a main component. It relates to a method for producing a novel food or drink characterized by being added or a food or drink having an anti-stress action.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION Anserine, valenin, π-methylhistidine and τ-methylhistidine used in the present invention are natural products, such as broth discharged during the production of skipjack tuna or dried sardines, and during the production of canned tuna. Extracted from cheap broth or inexpensive raw materials such as waste chicken
It may be separated and purified, chemically or enzymatically synthesized, or produced by a microorganism. Further, the anserine, varenin, π-methylhistidine and τ-methylhistidine of the present invention may be any of D-form, L-form and DL-form. Since naturally occurring anserine, valenin, π-methylhistidine and τ-methylhistidine are all L-forms,
When used as a food or drink, L-form is preferable.

The anserine, valenin, π-methylhistidine and τ-methylhistidine used in the present invention may be in the form of a salt, and as anserine salt, valenin salt, π-methylhistidine salt and τ-methylhistidine salt. Is a salt based on a carboxylic acid group, an acid addition salt based on an amino group and a pharmacologically acceptable acid, and a salt based on both a carboxylic acid group and an amino group.

Salts based on carboxylic acid groups include sodium,
There are salts with metals such as potassium, calcium, magnesium, zinc and aluminum, ammonium salts and substituted ammonium salts, for example salts with trialkylamines and other amines such as triethylamine, and salts based on amino groups include hydrochloric acid, There are salts with inorganic acids and organic acids such as sulfuric acid, phosphoric acid, acetic acid, propionic acid, lactic acid, tartaric acid, citric acid, succinic acid, maleic acid, benzenesulfonic acid and toluenesulfonic acid, which are known per se. The method can be prepared by reacting free anserine, valenin, π-methylhistidine, τ-methylhistidine with a stoichiometrically calculated amount of the selected acid or base. These may be used alone or in combination.

In the present invention, the anserine, valenin, π-methylhistidine, and τ-methylhistidine of the present invention are not necessarily limited to high-purity purified products, and the extract containing the imidazole compound of the present invention as a main component is used. It can also be used. Π-Methylhistidine or τ of the present invention
-Methylhistidine is an amino acid constituting anserine and valenin, respectively, and is considered to have the same effect as anserine and valenin.

The extract containing the imidazole compound as a main component of the present invention means, for example, broth discharged during the production of bonito flakes or dried sardines, concentrated liquid of broth discharged during the production of canned tuna, chicken and the like. An extract extracted according to a conventional method, or a broth discharged during the production of skipjack tuna or dried sardines, or a fraction containing the imidazole compound of the present invention separated according to a conventional method from the broth discharged during the production of canned tuna Etc. can be mentioned.

Particularly, the fraction from the broth discharged during the production of canned tuna is preferable because it contains abundant amounts of anserine and π-methylhistidine. The imidazole compound content of the imidazole compound-based extract of the present invention is 2% or more, preferably 10% or more, more preferably 50% or more, and particularly the anserine content is 1% or more, preferably 5% or more. % Or more, more preferably 20% or more.

The anti-stress composition of the present invention is used for stress during transient mental activities such as calculation and memory, and involuntary stress or physical stress such as involuntary stress that cannot be solved by one's own ability. Against the physical stress, there is an anti-stress action to alleviate this, maintain homeostasis of the living body, and therefore the compound of the present invention is useful for preventing stress or promoting recovery from stress, Preventive improvement of medical symptoms caused by stress can be expected.

As medical symptoms caused by stress,
Cardiovascular diseases such as arteriosclerosis, ischemic heart disease (angina, myocardial infarction), essential hypertension, cardiac neurosis, arrhythmia,
Respiratory diseases such as bronchial asthma, hyperventilation syndrome, neurotic cough, peptic ulcer, ulcerative colitis, irritable bowel syndrome,
Gastrointestinal disorders such as anorexia nervosa, nervous emesis, abdominal distension, aerial swallowing, and endocrine metabolism disorders such as obesity, diabetes, psychogenic polydipsia, and Graves' disease, migraines, Nervous system diseases such as myotonic headache, autonomic imbalance, urinary system diseases such as nocturnal enuresis, impotence, irritable bladder,
Rheumatoid arthritis, systemic myalgia, bone and muscle disorders such as spinal hypersensitivity, neurodermatitis,

Alopecia areata, hyperhidrosis, eczema and other skin system diseases, otolaryngological diseases such as Meniere syndrome, foreign body sensation in the throat, hearing loss, tinnitus, motion sickness and stuttering, and primary diseases Ophthalmological diseases such as glaucoma, asthenopia, blepharospasm, and eye hysteria, and obstetrics and gynecology areas such as dysmenorrhea, amenorrhea, menstrual abnormalities, functional uterine bleeding, menopause, insensitivity and infertility. Diseases, orthostatic dysregulation, recurrent umbilical colic, psychogenic fever, night terrors, and other pediatric diseases, intestinal adhesions, dumping syndrome, frequent surgery (polysurgery), plastic surgery Pre- and post-surgery conditions such as neurosis, idiopathic glossodynia, certain types of stomatitis, halitosis, abnormal salivation, check of masseter muscle, denture neuropathy and other diseases in the oral area, neurosis, depression, etc. be able to.

Further, the composition of the present invention can be used as a medicine or a functional food. When the antistress composition of the present invention is used as a medicine, the dosage form may be any dosage form as long as oral administration or parenteral administration is conveniently carried out, for example, injection solution, infusion solution,
Powders, granules, tablets, capsules, enteric agents, troches,
Examples include internal solutions, suspensions, emulsions, syrups, external solutions, poultices, nasal drops, ear drops, eye drops, inhalants, ointments, lotions, suppositories, etc. They can be used alone or in combination depending on the symptoms. These various preparations are known auxiliary agents that can be usually used in the technical field of pharmaceutical preparation such as an excipient, a binder, a disintegrating agent, a lubricant, a corrigent, a stabilizer, etc. as a main drug according to the purpose according to a conventional method. Can be used for formulation.

The dose of the anti-stress agent of the present invention varies depending on the route of administration, dosage form, symptoms, age, body weight, etc. Usually, when administered orally to adults, the total amount of the imidazole compound of the present invention is 50 mg to 5 g / day, preferably 1
00 mg to 2 g / day, more preferably 50 mg to 2 g / day,
More preferably, it is 50 mg to 500 mg / day. Also, as anserine and / or varenin, 50 mg to 5 g / day,
Preferably 100 mg to 2 g / day, more preferably 50 mg
˜2 g / day, more preferably 50 mg to 500 mg / day, 75 mg to 7.5 g / day, preferably 150 as π-methylhistidine and / or τ-methylhistidine.
mg-3g / day, more preferably 75mg-3g / day, more preferably 75mg-750mg / day.

For parenteral administration, the total amount of the imidazole compound of the present invention is 5 mg to 500 mg / day, preferably 1
0 mg to 200 mg / day, more preferably 5 mg to 200 mg /
Day, more preferably 5 mg to 50 mg / day. Also, as anserine and / or valenin, 5 mg to 500 mg /
Day, preferably 10 mg to 200 mg / day, more preferably 5 mg to 200 mg / day, even more preferably 5 mg to 50 mg / day
It is a day, and is 7.5 mg to 750 mg / day, preferably 15 mg to 300 mg / day, more preferably 7.5 mg to 30 as π-methylhistidine and / or τ-methylhistidine.
The dose is 0 mg / day, more preferably 7.5 mg to 75 mg / day.

When the anti-stress composition of the present invention is used as a functional food, it may be in the form of the above-mentioned pharmaceutical preparation, but it is mainly composed of anserine, valenin, π-methylhistidine or τ-methylhistidine or these. The extract as a component may be used alone or in combination to provide a protein (as a protein source, a highly nutritious milk protein with a balanced amino acid, soy protein, egg white oligopeptide, soybean hydrolyzate, etc., or a mixture of single amino acids. Are also used), sugars, fats, trace elements, vitamins, emulsifiers, flavors and the like, and processed into natural liquid foods, semi-digested nutritional foods, component nutritional foods and drinks.

Further, the imidazole compound of the present invention, or an extract containing the imidazole compound as a main component, is used in the form of the above-mentioned pharmaceutical preparation,
For example, it can be used as a drink or a capsule. Also, under the management of a dietitian based on a doctor's meal,
It is also possible to add the powder of the imidazole compound of the present invention to any food during the preparation of a hospital meal and give it to the patient in the form of a functional food prepared in situ. Furthermore, the functional food of the present invention comprises the required amount of anserine, valenin, π of the present invention.
-Methylhistidine, τ-methylhistidine or an extract containing them as a main component may be added to the food and drink raw material and processed and manufactured by a general manufacturing method. The blending amount varies depending on the dosage form and morphological characteristics of food, but generally 0.00
It is preferably 1 to 50%, but is not particularly limited.

The form of the food is solid or liquid food or a favorite product such as bread, noodles, rice, confectionery (biscuits, cookies, cakes, candy, chocolate, chewing gum, Japanese confectionery), tofu and processed products thereof. Agricultural foods, fermented foods such as sake and medicinal liquor, mirin, vinegar, soy sauce, miso, dressing, yogurt, ham, bacon, sausages, mayonnaise and other livestock foods, fish products such as kamaboko, fried tempura, hanpen, fruit juice It can be in the form of beverages, soft drinks, sports beverages, alcoholic beverages, beverages such as tea, and the like.

The imidazole compound of the present invention can be used in combination with vitamins, hormones and other nutritional supplements, as well as trace elements and iron compounds. Further, the functional food of the present invention prevents stress, or accelerates recovery from stress, or for the purpose of preventing or improving medical symptoms caused by stress, the total amount of the imidazole compound of the present invention per day 50 mg or more, preferably 100
It is desirable that orally ingested in an amount of mg or more, more preferably 50 mg to 5 g, more preferably 100 mg to 2 g, more preferably 50 mg to 2 g, and further preferably 50 mg to 500 mg.

Further, as anserine and / or valenin, 50 mg or more per day, preferably 100 mg or more, more preferably 50 mg to 5 g, and further preferably 100 mg to 2
g, more preferably 50 mg to 2 g, still more preferably 5
It is desirable to be ingested orally in the range of 0 mg to 500 mg. Further, as π-methylhistidine and / or τ-methylhistidine, 75 mg or more per day, preferably 15
0 mg or more, more preferably 75 mg to 7.5 g, more preferably 150 mg to 3 g, more preferably 75 mg to 3 g,
More preferably, it is desirable to be ingested orally in the range of 75 mg to 750 mg.

The anserine, valenin, π-methylhistidine and τ-methylhistidine of the present invention are substances originally existing in the body, and therefore have low toxicity and high safety, and therefore have great significance as an anti-stress composition. . Anserine, Valenin, π-Methylhistidine and τ-of the present invention
To foods and drinks that do not substantially contain methylhistidine, one or more imidazole compounds selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine, or an extract containing this as a main component is added. In the new food and drink, anserine, valenin, π-methylhistidine and τ-methylhistidine as foods and drinks substantially free of, for example, beef, pork, chicken, fish,
There are foods and drinks that do not use meats such as whale meat and snake meat, and foods and drinks that do not use meat extracts such as beef, pork, chicken, fish meat, whale meat, and snake meat.

Even in the case of foods and drinks made from meat such as beef, pork, chicken, fish, whale and snake meat, the product content of anserine, valenin, π-methylhistidine, τ-methylhistidine is very small. Therefore, the total amount of anserine, valenin, π-methylhistidine, τ-methylhistidine per daily intake is 50 mg or less, or 10
0 mg or less, 150 mg or less, 200 mg or less, anserine content per daily intake of 50 mg or less, 100 mg or less, 150 mg or less, or 20
0 mg or less is anserine, varenin, π of the present invention.
-Methylhistidine and τ-Methylhistidine are included in foods and drinks that do not substantially contain them.

The novel foods and drinks of the present invention are prepared by adding a required amount of anserine, varenin, π-methylhistidine or τ-methylhistidine of the present invention or an extract containing them as a main component to a food or drink raw material. It can be processed and manufactured by the manufacturing method. The blending amount varies depending on the dosage form and the morphological characteristics of the food, but is generally preferably 0.001 to 50%, but is not particularly limited. The form of the food can be the form of the functional food described above. Also, for the purpose of preventing physical or mental stress, promoting recovery from stress, or preventing or improving medical symptoms caused by stress, it is orally taken according to the above-mentioned functional food intake. be able to.

[0030]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Embodiment 1 FIG. Using 7-week-old female ICR mice, L-anserin was subcutaneously administered once a day at a dose of 100 mg / kg for 5 days, and physiological saline was subcutaneously administered once a day at a dose of 10 ml / kg for 5 days. Regarding the group, the stress load level was evaluated using the blood insulin secretion amount on the second day after the 20-hour restraint stress load as an index.

As shown in FIG. 1, the amount of insulin secreted in the physiological saline administration group decreased to 34.7% of the normal value due to the restraint stress, but the amount of insulin secreted in the beverage administration group containing anserine was decreased. 72.5% of normal value
Met. L-anserin was shown to have an anti-stress effect, as it significantly increased insulin secretion, which was reduced by involuntary mental stress.

Example 2. Using a 7-week-old female ICR mouse, chicken extract (BRA containing 2.1 mg / ml of anserine)
ND'S TRADITIONAL ESSENCE OF CHICKEN (BEC): Cerebos
Pacific Ltd.) was orally administered at a dose of 10 ml / kg once a day for 5 days, and water was orally administered at a dose of 10 ml / kg once a day for 5 days, after 20 hours restraint stress loading. The stress load level was evaluated using the amount of insulin secretion in blood on the second day as an index.

As shown in FIG. 2, the amount of insulin secretion in the water-administered group was 2% of the normal value due to the load of restraint stress.
Although it decreased to 2.0%, the insulin secretion amount in the beverage administration group containing anserine was 42.0% of the normal value. Significantly increased insulin secretion decreased by involuntary mental stress, indicating that the anserine-containing material has an anti-stress effect.

Embodiment 3 FIG . Using 7-week-old female ICR mice, the same chicken extract (BEC) as in Example 1 was orally administered at a dose of 10 ml / kg once a day for 5 days, and water was administered at a dose of 10 ml / kg once a day. For the group that was orally administered for 5 days, the stress load was evaluated using the glucose tolerance (glucose load test) on the 2nd day after the 20-hour restraint stress load as an index.

[0035]

[Table 1] As shown in Table 1, the half-life of the loaded sugar in the water-administered group was extended to 181% of the normal value by the load of restraint stress, but the half-life of the loaded sugar in the beverage-administered group containing anserine was the normal value. It was 139%. Also, the blood elimination rate of sugar in the water-administered group (Clearance R
ate) decreased to 54.5% of the normal value, but the blood elimination rate of sugar in the beverage administration group containing anserine was 91.6% of the normal value. It was shown that the anserine-containing substance has an anti-stress effect because it shortens the half-life of loaded sugar that is prolonged by involuntary mental stress and enhances the blood elimination rate of the lowered sugar.

Example 4. Using 7-week-old female ICR mice, the same chicken extract (BEC) as in Example 1 was orally administered at a dose of 10 ml / kg once a day for 5 days, and water was administered at a dose of 10 ml / kg once a day. GOT on the 2nd day after 20 hours restraint stress in the group administered orally for 5 days
The stress load was evaluated using the concentration as an index. As shown in FIG. 3, the serum G in the water-administered group was changed by the load of restraint stress.
The OT concentration significantly increased to 232% of the normal value, but the serum GOT concentration of the beverage administration group containing anserine was 165% of the normal value. It was shown that the anserine-containing material had an anti-stress effect, as it tended to lower the serum GOT concentration increased by involuntary mental stress.

Example 5. Example 1 containing 150 mg of anserine per 16 healthy male university students
Computational power between the case where the same chicken extract (BEC) was drunk once a day for 1 week, and the aqueous solution of anserine-free gelatin (placebo) was taken twice a day for 1 week. Tests and short-term memory tests were carried out to evaluate the stress load induced by this mental activity from the serum cortisol level, which is a substance related to blood stress.

As shown in FIG. 4, the anserine-containing material (B
It was observed that when EC) was taken, the speed at which the serum cortisol level increased due to stress due to excessive mental activity recovered to a normal value significantly faster than when the placebo was taken. This means that the anserine-containing material exhibited an anti-stress effect that accelerates recovery from stress caused by excessive mental activity.

Example 6. The concentrated liquid of skipjack broth was treated with an ultrafiltration membrane for fractionating at a molecular weight of 10,000 to obtain a clarified liquid of a low molecular fraction. After adjusting the pH of the clarified liquid to 2.2 with dilute hydrochloric acid, this liquid was poured into a column packed with MM81 type ion exchange resin, manufactured by Beckman, and anserine, π
-Methylhistidine was adsorbed. Then the column is 57
A 0.4N sodium citrate solution was allowed to flow while being kept warm at 0 ° C to obtain a fraction containing anserine and π-methylhistidine. This was freeze-dried to obtain a dried product. Anserine or π of this dried product was analyzed by an amino acid analyzer.
-Methylhistidine content was measured to be 5 each
It was 6% and 8%.

Example 7. Lactose 50 parts, sucrose 39.8 parts,
Mix 5 parts of tragacanth gum and 0.2 parts of peppermint,
A solution prepared by dissolving 5 parts of the dried product obtained in Example 6 in 3.5 parts of distilled water in terms of the total amount of anserine and π-methylhistidine was added and kneaded well. Then, the above kneaded product was spread on a glass plate on which starch was sprayed with a rolling pin to form a sheet having a thickness of about 5 mm, which was punched out with a mold and dried to obtain a troche.

Example 8. 100 parts of flour with anserine 2
Parts, sucrose 4 parts, salt 1.1 parts, skim milk powder 2 parts, yeast 3 parts, yeast food 0.8 parts, and water 67.2 parts were added and kneaded, and then 5 parts of oil and fat were further added and kneaded well. 9 at 28 ° C
A 0-minute primary fermentation was performed. After that, punching was performed, and second fermentation was performed at 28 ° C. for 20 minutes, and then the mixture was packed in a mold and baked at 190 ° C. for 30 minutes to manufacture. The physical properties of the baked bread were compared with those of the bread produced without the addition of anserine, and as a result, no difference was found in flavor and texture.

Example 9. BRAND'S TRADIT on 80 parts of pork
2 parts of IONAL ESSENCE OF CHICKEN (Cerebos Pacific Ltd.) converted to anserine, 0.02 parts of sodium nitrite,
Add 2 parts salt, 0.3 parts sodium pyrophosphate, 0.006 parts sodium ascorbate, 1 part sucrose, and add 10 ° C.
After 72 hours of salting, a seasoning and a spice were added and the mixture was cut to prepare a ground meat. After filling this kneaded meat in a casing, a sausage was produced according to a conventional method. As a result of sensory inspection of this sausage, BRAND'S TRADITIONAL ESSENC
No difference in color tone, flavor, or texture was observed as compared to sausage produced without the addition of EOF CHICKEN.

Example 10. Using the synthesized anserine, granules were manufactured by the usual method according to the following formulation. Anserine 20 parts Lactose 34 parts Corn starch 45 parts Hydroxypropyl methylcellulose 1 part

Example 11. To 20 g of vitamin C, 10 g of ferrous sulfate, 40 g of granulated sugar, 30 g of an equal mixture of corn starch and lactose, 50 g of the extract obtained in Example 6
In addition, they were mixed. The mixture is divided into 100 equal parts and packed in a bag. 1
100 stick-shaped functional foods having a learning ability of 1.5 g were manufactured.

[Brief description of drawings]

FIG. 1 is a graph showing the effect of L-anserin on blood insulin concentration during restraint stress.

FIG. 2 is a graph showing the effect of anserine-containing chicken meat extract on blood insulin concentration under restraint stress.

FIG. 3 is a graph showing the effect of anserine-containing chicken meat extract on serum GOT concentration under restraint stress.

FIG. 4 is a graph showing the effect of anserine-containing chicken extract on serum cortisone concentration in stress caused by mental activity.

Claims (11)

[Claims] 1. An anti-stress composition comprising one or more imidazole compounds selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine. 2. The anti-stress composition according to claim 1, which is a pharmaceutical product. 3. The anti-stress composition according to claim 1, which is a functional food. 4. The anti-stress composition according to claim 3, wherein the functional food is a food or drink in a form in which 50 mg to 5 g / day of an imidazole compound can be ingested. 5. The anti-stress composition according to claim 1, wherein the imidazole compound is anserine. 6. A food or drink substantially free of anserine, valenin, π-methylhistidine and τ-methylhistidine, wherein at least one member selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine is used. A novel food or drink product to which an imidazole compound or an extract containing this as a main component is added. 7. A food or drink having an anti-stress effect,
The food or drink according to claim 6. 8. The food or drink according to claim 6 or 7, which is in a form in which 50 mg to 5 g / day of an imidazole compound can be ingested. 9. A food or drink substantially free of anserine, valenin, π-methylhistidine and τ-methylhistidine, wherein at least one member selected from the group of anserine, valenin, π-methylhistidine and τ-methylhistidine is used. A novel method for producing a food or drink, which comprises adding an imidazole compound or an extract containing the imidazole compound as a main component. 10. The method according to claim 9, wherein the food or drink is a food or drink having an anti-stress action. 11. The method according to claim 9, wherein the food or drink is a food or drink in a form capable of ingesting 50 mg to 5 g / day of an imidazole compound.