JPH09154589A - Production of erythritol - Google Patents

Abstract

PROBLEM TO BE SOLVED: To profitably obtain a compound from an inexpensive raw material by culturing a microorganism belonging to the genus Moniliella or Trichosporonoides and capable of producing the erythritol from a fermentable saccharide and subsequently collecting the product from the culture product. SOLUTION: This method for producing the erythritol comprises culturing a microorganism (e.g. Moniliella actobutens CBS 166.66) belonging to the genus Moniliella excluding Moniliella tomentosa var. pollinis or to the genus Trichosporonoides and having an ability to produce the erythritol from a fermentable saccharide in a culture medium containing the fermentable saccharide such as glucose, fructose or glycerol as a main carbon source in a concentration of 20-60% at a culture temperature of 25-37 deg.C and subsequently collecting the product from the culture product. Thus, the objective erythritol is efficiently obtained from the inexpensive raw material such as the fermentable saccharide in a high yield.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for producing erythritol, and more particularly to a method for industrially advantageously producing erythritol by fermentation using a microorganism.

[0002]

2. Description of the Related Art As a method for producing erythritol, a method of producing microorganisms of the genera Trigonobosis and Candida by culturing them in a medium containing glycerol as a carbon source (Japanese Patent Publication No. Sho 4).
No. 7-41549), Candida, Tolulopsis, and Hansenula microorganisms are cultured in a medium containing a hydrocarbon or the like as a carbon source to produce (Japanese Patent Publication No. 51-2107).
No. 2) is known. However, these methods have not yet been industrialized because the raw material used as a carbon source is not suitable for actual industrial production.

In addition, Moniliella Tomentosa Pearl
There is also known a method (Japanese Patent Laid-Open No. 60-110295) for producing a polynis by culturing it in a medium containing a sugar such as glucose as a carbon source. This method uses glucose, which is a cheap and safe raw material, and is excellent in that it has high productivity, but foaming during culture is remarkable, and it is useless with a defoaming agent that is usually used. However, since it is necessary to add a large amount of expensive xadan gum and the like, it is not always an advantageous method in industrial production.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for producing erythritol from a raw material that is inexpensive and can be easily supplied at a high yield and at a low cost.

[0005]

Means for Solving the Problems As a result of earnest research on a method for producing erythritol from an inexpensive raw material, the present inventors have found that many microorganisms belonging to the genus Moniliella and the genus Trichosporonoides are found to have glucose, fructose and the like. It was found that a large amount of erythritol was produced from fermentable sugar, and the present invention was completed.

That is, the present invention uses a fermentable sugar as a main carbon source for a microorganism belonging to the genus Moniliella or the genus Trichosporonoides excluding Moniliella tomentosa pearl polynis and having the ability to produce erythritol from fermentable sugar. The present invention provides a method for producing erythritol, which comprises culturing the erythritol from the culture medium and collecting erythritol from the culture.

Furthermore, according to a preferred embodiment of the present invention,
The above-mentioned production method in which the microorganism belonging to the genus Moniliella is Moniliella acetoabten or Moniliella suaveolens; the microorganism belonging to the genus Trichosporonoides is Trichosporonoides oedsephalis, Trichosporonoides megachiriensis, Trichosporonoides. Media, the above production method which is Trichosporonoides nigrescens or Trichosporonoides spaslata; the above production method wherein the fermentable sugar is glucose, fructose or glycerol; The concentration of fermentable sugar in the medium is 20. The above-mentioned production method is in the range of -60%; The above-mentioned production method is provided in which the culture temperature is in the range of 25 to 37 ° C.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below. The microorganism used in the present invention includes the genus Moniliella or Trichosporonoides.
There is no particular limitation as long as it is a microorganism belonging to the genus Sporonoides and having the ability to produce erythritol from fermentable sugars, and any microorganism can be used.

Examples of the microorganisms belonging to the genus Moniella include Moniliella acetoabten.
utens ) and Moniliella Suavelens ( Moniliell)
a suaveolens ), and specific strains include, for example, Moniliella acetoabten CBS16.
9.66, Moniliella Suavelens CBS12
6.42 and Moniliella Suavelens CBS12
0.67 etc. can be mentioned.

[0010] Examples of the microorganism belonging to the Trichosporonoides genus, for example, Trichosporonoides-Oedosefarisu (Trichosporonoides oedocephalis), Trichosporonoides megachiliensis (Trichosporonoides me
gachiliensis ), Trichosporonoides Media ( Tr
ichosporonoides madida), Trichosporonoides-nigrescens (Trichosporonoides nigrescens) and Trichosporonoides-Supasurata (Trichosporonoides
spathulata ) and the like. Specific strains include, for example, Trichosporonoides oedsephalis CBS649.66, Trichosporonoides megachiriensis CBS567.85, Trichosporonoides.
Media CBS240.79, Trichosporonoides
Nigressence CBS268.81 and Tricosporonoides spaslata CBS241.79 can be mentioned.

These strains are known as Centraal Bureau voor Schimmelcultures (C
It has been deposited with BS and is easily available. Fermentable sugars such as glucose, fructose and glycerol are used as the main carbon source of the medium used for culturing the above-mentioned microorganisms. These carbon sources can be used alone or in combination. The concentration to be used is not particularly limited, but it is advantageous to make it as high as possible within a range that does not inhibit the production of erythritol. The preferred concentration is within the range of 20-60% (W / V).

As the nitrogen source, various organic and inorganic nitrogen compounds such as ammonia salt, urea, peptone, microbial extract and corn stapler are used. As the inorganic salt, various phosphates, sulfates, and metal salts such as magnesium, potassium, manganese, iron, and zinc are used. In addition, factors such as vitamins, nucleotides and amino acids that promote the growth of microorganisms can be added as necessary. Also,
It is desirable to add an appropriate amount of a commercially available antifoaming agent in order to suppress foaming during culturing.

At the time of culturing, cells may be directly inoculated from the slant culture to the medium, but it is preferable to inoculate a preculture obtained by culturing in a liquid medium for 1 to 4 days. The medium at the beginning of the culture has a pH of 3 to 7, preferably pH 3 to 4.
Adjust to 5. The culture temperature is 25 ° C to 37 ° C, preferably 27 ° C to 35 ° C. Also, culture is aeration and agitation,
It is desirable to perform it under aerobic conditions such as shaking. Culturing time is preferably performed until the main carbon source is consumed, and usually 3 to 8 days. The amount of erythritol produced in the culture broth can be measured by a method such as gas chromatography or high performance liquid chromatography.

The erythritol thus accumulated in the culture medium is separated and purified from the culture according to a conventional method. Specifically, after removing solids by centrifugation, filtration, etc., decolorization and desalting with activated carbon and ion exchange resin,
Erythritol can be separated and purified by crystallization from the solution.

[0015]

The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples. Examples 1 to 8 50 ml of a medium containing 30% glucose (W / V) and 1% yeast extract was placed in a 500 ml Erlenmeyer flask having a cotton plug and sterilized at 120 ° C. for 20 minutes. In this medium, Moniliella acetoabten CBS169.66 strain, Moniliella suaveolen CBS126.42 strain, Moniliella suaveolens CBS120.67 strain, Trichosporonoides odecephalis CBS649.66 strain.
Ltd., Tricos Poronoides Media CBS 240.7
9 strains, Trichosporonoides Nigressens CBS2
68.81 shares, Trichosporonoides Spaslata CB
S241.79 strain and Trichosporonoides megachiriensis CBS567.85 strain were inoculated respectively, and 27
The culture was carried out at 10 ° C with shaking for 10 days. After the culture was completed, the concentration of erythritol in the culture was measured by high performance liquid chromatography.

As a result, the amount of erythritol produced by each strain was as follows. Example No. Strain Erythritol Production Example 1 CBS 169.66 35.9 g / L Example 2 CBS 126.42 30.4 g / L Example 3 CBS 120.67 62.2 g / L Example 4 CBS 649.66 103.4 g / L Example 5 CBS 240.79 107.4 g / L Example 6 CBS 268.81 136.0 g / L Example 7 CBS 241.79 40.5 g / L Example 8 CBS 567.85 58.5 g / L

[0017]

EFFECTS OF THE INVENTION According to the present invention, erythritol can be efficiently produced in high yield from inexpensive fermentable sugars such as glucose.

Claims (6)

[Claims] 1. A microorganism having the ability to produce erythritol from fermentable sugars belonging to the genus Moniliella or the genus Trichosporonoides excluding Moniliella tomentosa pearl polynis and having fermentable sugar as a main carbon source. A method for producing erythritol, which comprises culturing in a medium and collecting erythritol from the culture. 2. The production method according to claim 1, wherein the microorganism belonging to the genus Moniliella is Moniliella acetoabten or Moniliella saveolens. 3. A microorganism belonging to the genus Trichosporonoides belongs to Trichosporonoides oedsephalis, Trichosporonoides megachiriensis, Trichosporonoides media, Trichosporonoides nigrescens or Trichosporonoides spaslata. Claim 1
The production method described in 1. 4. The production method according to claim 1, wherein the fermentable sugar is glucose, fructose, or glycerol. 5. The concentration of fermentable sugar in the medium is 20 to 60.
The manufacturing method according to claim 1, which is within the range of%. 6. The production method according to claim 1, wherein the culture temperature is in the range of 25 ° C. to 37 ° C.