JP2003180391A - METHOD FOR PRODUCING epsilon-POLY-L-LYSINE - Google Patents
METHOD FOR PRODUCING epsilon-POLY-L-LYSINEInfo
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- JP2003180391A JP2003180391A JP2001390102A JP2001390102A JP2003180391A JP 2003180391 A JP2003180391 A JP 2003180391A JP 2001390102 A JP2001390102 A JP 2001390102A JP 2001390102 A JP2001390102 A JP 2001390102A JP 2003180391 A JP2003180391 A JP 2003180391A
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- fermentation
- poly
- lysine
- producing
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、ε―ポリ−Ｌ−リ
ジン（以下εＰＬという）の製造法に関する。さらに詳
しくは、ε―ポリ−Ｌ−リジンを発酵生産するストレプ
トマイセス（Streptomyces）属微生物を好気的に培地に
培養して、ε―ポリ−Ｌ−リジンを採取する方法におい
て、発酵終了後に発酵上澄み液と菌体を分離させるまで
の間、常に発酵終了後の発酵液中に炭素源を残存させて
おくことを特徴とするε―ポリ−Ｌ−リジンの製造法に
関する。TECHNICAL FIELD The present invention relates to a method for producing ε-poly-L-lysine (hereinafter referred to as εPL). More specifically, in a method of aerobically culturing a microorganism of the genus Streptomyces that fermentatively produces ε-poly-L-lysine in a medium and collecting ε-poly-L-lysine, after fermentation is completed, The present invention relates to a method for producing ε-poly-L-lysine, which is characterized in that a carbon source is always left in the fermentation liquor after fermentation until the fermentation supernatant and the bacterial cells are separated.
【０００２】εＰＬは、必須アミノ酸であるＬ−リジン
のポリマーであるため安全性が高く、かつカチオン含量
が高いので特異な物性を有する。したがって、トイレタ
リー用品、化粧品、医薬、農薬、食品添加物、電子材料
等への用途が期待されている。特に食品添加物の分野で
は、天然物系の添加物として大きく注目されている。Since εPL is a polymer of L-lysine which is an essential amino acid, it is highly safe and has a high cation content, and therefore has unique physical properties. Therefore, it is expected to be used for toiletries, cosmetics, medicines, agricultural chemicals, food additives, electronic materials and the like. In particular, in the field of food additives, it has received a great deal of attention as a natural product-based additive.
【０００３】[0003]
【従来の技術】従来の該εＰＬの発酵製造法では、発酵
中の炭素源濃度については記載されているが（特開平10
-174596号公報）、発酵終了後の発酵液中の炭素源濃度
について着目したものは報告がない。通常、発酵製造法
においては、炭素源コストを削減するため、炭素源を使
い切る形で発酵反応を終了するか、もしくはごく少量の
炭素源を発酵液中に残して発酵反応を終了させるのが一
般的である。しかしながら、このような方法でストレプ
トマイセス属微生物を用いたεＰＬの発酵を行うと、発
酵液中のεＰＬ濃度が発酵終了後に低下する現象が起こ
り、εＰＬの生産性の低下を招くといった問題が生じ
る。特に発酵液量が多くなり、菌体除去工程に時間がか
かるようになると、発酵終了後の発酵液中のεＰＬ量の
低下が著しくなる。したがって、従来の製造法では、ε
ＰＬの生産性が未だ低く、種々の用途に対応しうるεＰ
Ｌを収率よく製造するのは困難であった。そこで、生成
したεＰＬの安定性を高めることにより、工業的に収率
のよい製造法が望まれていた。2. Description of the Related Art In the conventional method for producing εPL by fermentation, although the carbon source concentration during fermentation has been described (Japanese Patent Laid-Open No. H10 (1998) -109242).
No. 174596), there is no report focusing on the carbon source concentration in the fermented liquid after the fermentation. Usually, in the fermentation manufacturing method, in order to reduce the carbon source cost, it is common to finish the fermentation reaction by using up the carbon source, or leave the fermentation reaction by leaving a very small amount of carbon source in the fermentation liquid. Target. However, when εPL is fermented using a microorganism of the genus Streptomyces by such a method, a phenomenon occurs in which the εPL concentration in the fermentation liquor decreases after the fermentation is completed, which causes a problem that the productivity of εPL decreases. . In particular, when the amount of the fermentation liquor becomes large and the cell removal step takes a long time, the amount of εPL in the fermentation liquor after the fermentation is significantly decreased. Therefore, in the conventional manufacturing method, ε
The productivity of PL is still low, and εP can be used for various applications.
It was difficult to produce L in good yield. Therefore, there has been a demand for an industrially high-yield production method by increasing the stability of the produced εPL.
【０００４】[0004]
【発明が解決しようとする課題】本発明者らは、εＰＬ
を工業的に収率よく製造する方法について鋭意検討を重
ねた。その結果、εＰＬ生産能を有するストレプトマイ
セス属微生物を培養してεＰＬ発酵を行った後の発酵終
了後の発酵液においても、菌体を除去するまでの間は、
常に炭素源を残存させておくことによってεＰＬの減少
が阻止できることを見いだし、すなわち、安定した収率
でεＰＬを生産することができることを見出し、この知
見に基づいて本発明を完成した。以上の記述から明らか
なように、本発明の目的は、発酵終了後のεＰＬの生産
量の低下を抑制し、収率よくεＰＬを製造する方法を提
供することである。DISCLOSURE OF THE INVENTION The present inventors have found that εPL
The inventors have made earnest studies on a method for industrially producing sucrose. As a result, even in the fermented liquid after completion of fermentation after culturing Streptomyces microorganisms having εPL-producing ability and performing εPL fermentation, until removal of bacterial cells,
It was found that the decrease of εPL can be prevented by always leaving the carbon source, that is, εPL can be produced in a stable yield, and the present invention was completed based on this finding. As is clear from the above description, an object of the present invention is to provide a method for suppressing the decrease in the production amount of εPL after the completion of fermentation and producing εPL in a high yield.
【０００５】[0005]
【課題を解決するための手段】本発明は下記から構成さ
れる。
（１）ε―ポリ−Ｌ−リジンを発酵生産するストレプト
マイセス（Streptomyces）属微生物を好気的に培地に培
養して、ε―ポリ−Ｌ−リジンを採取する方法におい
て、発酵終了後に発酵上澄み液と菌体を分離させるまで
の間、常に発酵終了後の発酵液中に炭素源を残存させて
おくことを特徴とするε―ポリ−Ｌ−リジンの製造法。The present invention comprises the following: (1) In a method of aerobically culturing a microorganism of the genus Streptomyces that fermentatively produces ε-poly-L-lysine in a medium to collect ε-poly-L-lysine, fermentation is performed after the fermentation is completed. A method for producing ε-poly-L-lysine, which is characterized in that a carbon source is always left in the fermented liquid after completion of fermentation until the supernatant and the bacterial cells are separated.
【０００６】（２）発酵終了後の発酵液中の炭素源の残
存濃度が1g/リットル〜100g/リットルである前記第１項
記載のε―ポリ−Ｌ−リジンの製造法。(2) The method for producing ε-poly-L-lysine according to the above item 1, wherein the residual concentration of the carbon source in the fermented liquid after the fermentation is 1 g / liter to 100 g / liter.
【０００７】（３）発酵終了後の発酵液中の炭素源の残
存濃度が２g/リットル〜２0g/リットルである前記第１
項記載のε―ポリ−Ｌ−リジンの製造法。(3) The above-mentioned first, wherein the residual concentration of the carbon source in the fermented liquor after the fermentation is 2 g / liter to 20 g / liter
The method for producing ε-poly-L-lysine according to the item.
【０００８】（４）発酵液中に残存させておく炭素源
が、グルコース、フラクトース、グリセリン、スター
チ、ガラクトース、マンニトール、イノシトール、サリ
シンの中から選ばれた１種以上である前記第１項〜第３
項のいずれか１項記載のε―ポリ−Ｌ−リジンの製造
法。記載のε―ポリ−Ｌ−リジンの製造法。(4) The carbon source to be left in the fermentation broth is at least one selected from glucose, fructose, glycerin, starch, galactose, mannitol, inositol, and salicin. Three
Item 10. A method for producing ε-poly-L-lysine according to any one of items. Process for producing ε-poly-L-lysine as described.
【０００９】（５）ε―ポリ−Ｌ−リジンを発酵生産す
るストレプトマイセス（Streptomyces）属微生物が、ス
トレプトマイセス・アルブラス・リジノポリメラス(Str
eptomyces albulus subsp. lysinopolymerus)B21021株
（FERM BP−5926）である前記第１項記載のε―ポリ−
Ｌ−リジンの製造法。(5) A microorganism belonging to the genus Streptomyces that fermentatively produces ε-poly-L-lysine is Streptomyces albus lysinopolymerase (Str
eptomyces albulus subsp. lysinopolymerus) B21021 strain (FERM BP-5926), ε-poly-
A method for producing L-lysine.
【００１０】本発明に使用できる微生物は、εＰＬを生
産する能力のあるストレプトマイセス属微生物であれば
いずれでも使用可能である。かかるストレプトマイセス
属微生物の具体的な例としては、ストレプトマイセス・
アルブラス・リジノポリメラス(Streptomyces albulus
subsp. lysinopolymerus)B21021株（FERM BP−5926）
があげられる。Any microorganism can be used in the present invention as long as it is a microorganism belonging to the genus Streptomyces and capable of producing εPL. Specific examples of such Streptomyces microorganisms include Streptomyces
Albrus lysinopolymeras (Streptomyces albulus
subsp.lysinopolymerus) B21021 strain (FERM BP-5926)
Can be given.
【００１１】本発明の製造法にあっては、発酵終了後の
発酵中にも炭素源を残存させておくものであり、使用す
る炭素源としては、発酵中に使用した炭素源と同じもの
が好ましいが、発酵終了後に残存させる炭素源として
は、グルコース、フラクトース、グリセリン、スター
チ、ガラクトース、マンニトール、イノシトール、サリ
シン等のεＰＬ生産菌が資化可能なものならいずれも使
用できる。In the production method of the present invention, the carbon source is allowed to remain during the fermentation after the fermentation, and the carbon source used is the same as the carbon source used during the fermentation. Although preferred, any carbon source left after the fermentation can be used as long as it can assimilate εPL-producing bacteria such as glucose, fructose, glycerin, starch, galactose, mannitol, inositol, and salicin.
【００１２】本発明で使用する培地としては、炭素源、
窒素源、無機物及びその他の栄養素を適当に含有する培
地ならばいずれも使用できる。炭素源としては、グルコ
ース、フラクトース、グリセリン、スターチ、ガラクト
ース、マンニトール、イノシトール、サリシン等のεＰ
Ｌ生産菌が資化可能なものならいずれも使用できる。培
地中の残存炭素源濃度は、菌体の増殖及びεＰＬの生産
とともに低下する。残存炭素源濃度が低下したら、炭素
源を逐次的にもしくは連続的に添加してもかまわない。
また、窒素源としては有機体窒素や無機体窒素のいずれ
でもかまわないが、硫酸アンモニウム、塩化アンモニウ
ム等のアンモニア態窒素が最適である。培養液中の残存
窒素濃度が低下したら、炭素源と同様、窒素源を逐次的
にもしくは連続的に添加してもかまわない。無機物とし
ては、りん酸イオン、カリウムイオン、マグネシウムイ
オン、亜鉛イオン、鉄イオン、マンガンイオン、ナトリ
ウムイオン、カルシウムイオン等があげられる。その他
の栄養素として、酵母エキスを適当量含有させること
は、菌の生育を良くし、εＰＬの生産にも好ましい結果
を与える。The medium used in the present invention includes a carbon source,
Any medium can be used as long as it appropriately contains a nitrogen source, an inorganic substance and other nutrients. Carbon sources include εP such as glucose, fructose, glycerin, starch, galactose, mannitol, inositol and salicin.
Any L-producing bacterium that can be assimilated can be used. The residual carbon source concentration in the medium decreases with the growth of bacterial cells and the production of εPL. When the residual carbon source concentration decreases, the carbon source may be added sequentially or continuously.
The nitrogen source may be either organic nitrogen or inorganic nitrogen, but ammonium nitrogen such as ammonium sulfate and ammonium chloride is most suitable. When the residual nitrogen concentration in the culture solution decreases, the nitrogen source may be added sequentially or continuously, like the carbon source. Examples of the inorganic substance include phosphate ion, potassium ion, magnesium ion, zinc ion, iron ion, manganese ion, sodium ion, calcium ion and the like. The inclusion of an appropriate amount of yeast extract as another nutrient improves the growth of the bacterium and gives favorable results for the production of εPL.
【００１３】培養は、振とう培養、攪拌培養等の好気的
条件下で行う。培養温度は25℃〜35℃が好ましい。発酵
終了後の発酵液保存温度は25℃以下が好ましいが、さら
に好ましくは15℃以下である。培地のｐHは中性付近
（ｐH６〜８）が好ましいが、培養開始後に生産菌の生
育ともにｐHは低下する。ｐHが3.5以下、好ましくはｐH
４以下にならないように、アルカリを添加してｐHを維
持する。アルカリはｐHを維持できるものであれば、何
でもかまわないが、好ましくはアンモニア水である。通
常は、培養１〜７日間でεＰＬが蓄積される。The culture is carried out under aerobic conditions such as shaking culture and stirring culture. The culture temperature is preferably 25 ° C to 35 ° C. The storage temperature of the fermented liquid after the fermentation is preferably 25 ° C or lower, more preferably 15 ° C or lower. The pH of the medium is preferably around neutral (pH 6 to 8), but the pH decreases with the growth of the producing bacteria after the start of culture. pH is 3.5 or less, preferably pH
The pH is maintained by adding alkali so that the pH does not become 4 or less. Any alkali can be used as long as it can maintain pH, but ammonia water is preferable. Usually, εPL is accumulated in 1 to 7 days of culture.
【００１４】本発明の製造法にあっては、発酵終了後の
発酵液を遠心分離もしくはフィルタ−等による濾過によ
り、菌体を除去するまでの間、炭素源を残存させること
が特徴であるが、このときの炭素源の濃度は、発酵液
中、好ましくは１ｇ／リットル〜１００ｇ／リットル、
より好ましくは２ｇ／リットル〜２０ｇ／リットルであ
る。この濃度範囲に残存炭素源濃度を維持すれば生成ε
ＰＬ量の低下が抑えられ、収率よく、εＰＬを製造する
ことができる。The production method of the present invention is characterized in that the fermentation broth after completion of fermentation is centrifuged or filtered by a filter or the like to leave a carbon source until the bacterial cells are removed. The concentration of the carbon source at this time is preferably 1 g / liter to 100 g / liter in the fermentation liquid,
It is more preferably 2 g / liter to 20 g / liter. If the residual carbon source concentration is maintained within this concentration range, it will be generated ε
A decrease in the amount of PL is suppressed, and εPL can be produced in good yield.
【００１５】上記εＰＬ及び残存炭素源を含有する発酵
液は遠心分離もしくはフィルター等で菌体を除いた後、
菌体除去液を精製、脱色し、これを濃縮する。濃縮液か
らアセトン、エタノール等の有機溶媒で晶析することに
より、目的の該発酵液の保存中はグルコースを添加する
ことによりが得られる。The fermented liquor containing εPL and the residual carbon source is centrifuged or filtered to remove bacterial cells,
The microbial cell removal liquid is purified, decolorized, and concentrated. By crystallization from the concentrated solution with an organic solvent such as acetone or ethanol, it can be obtained by adding glucose during storage of the desired fermentation solution.
【００１６】[0016]
【実施例】本発明を実施例により更に詳細に説明する。
尚、培養液中のεＰＬ濃度は、イツアキ（Itzhaki）：
アナリティカルバイオケミストリー（Analytical Bioch
emistry）50,569,(1972)の方法により測定した。すなわ
ち、培養液を遠心分離して菌体を除いた後、菌体除去液
（εＰＬ：0〜200μg）２mlと１mMメチルオレンジ水溶
液２mlとを混合し、室温で３０分間放置してεＰＬ―メ
チルオレンジコンプレックスを生じさせる。その後、遠
心することにより、該εＰＬ―メチルオレンジコンプレ
ックスを除いた上澄水の465nmにおける吸光度を測定
し、εＰＬ量を求めた。また、実施例中の％は特に断ら
ない限り、重量(g)／容量(dl)％である。EXAMPLES The present invention will be described in more detail by way of examples.
In addition, the εPL concentration in the culture medium was determined by Itzhaki:
Analytical Bioch
emistry) 50,569, (1972). That is, after the culture solution was centrifuged to remove the cells, 2 ml of the cell removal solution (εPL: 0 to 200 μg) and 2 ml of a 1 mM methyl orange aqueous solution were mixed and allowed to stand at room temperature for 30 minutes to obtain εPL-methyl orange. Give rise to a complex. Then, by centrifuging, the absorbance at 465 nm of the supernatant water from which the εPL-methyl orange complex was removed was measured to determine the εPL amount. In addition,% in the examples is weight (g) / volume (dl)% unless otherwise specified.
【００１７】実施例１
グルコース50g/リットル、酵母エキス5g/リットル、硫
酸アンモニウム10g/リットル、K2HPO4 0.8g/リットル、
KH2PO4 1.36g/リットル、MgSO4・7H2O 0.5g/εＰＬ、 Zn
SO4・7H2O 0.04g/リットル、 FeSO4・7H2O 0.03g/リット
ル、pH6.8に調製した２リットルの培地を３リットル容
ジャーに入れ、これにストレプトマイセス・アルブラス
・サブスピーシズ・リジノポリメラス（Streptomyces a
lbulus subsp．lysinopolymerus）B21021株（FERM BP-
5926号）の前培養液100mLを接種し、30℃、700rpm、通
気量３リットル/分で3日間培養を行った。ただし、培養
開始後、培養液中の残存グルコース濃度が10g/リットル
以下になった時点で、残存グルコース濃度が50g/リット
ルになるようにグルコースを逐次添加した。硫酸アンモ
ニウムについても、グルコース添加と同時にグルコース
の1/10倍量（重量比）を逐次添加した。ｐH調整につい
ては、10％アンモニア水を用いてpHを４に維持した。
尚、培養３日後の発酵終了時点での残存グルコース濃度
は20g/リットルであった。この発酵液を、通気だけを行
いながら室温で24時間保存した。発酵終了時及び24時間
保存後のεＰＬ濃度とグルコース濃度を表１に示した。Example 1 Glucose 50 g / liter, yeast extract 5 g / liter, ammonium sulfate 10 g / liter, K 2 HPO 4 0.8 g / liter,
KH 2 PO 4 1.36g / l, MgSO 4 · 7H 2 O 0.5g / εPL, Zn
SO 4 · 7H 2 O 0.04g / l, FeSO 4 · 7H 2 O 0.03g / l, the medium was placed in a 2 liter was prepared pH6.8 to a 3 liter jar, which Streptomyces albulus ssp LysinoPolymerus (Streptomyces a
lbulus subsp. lysinopolymerus) B21021 strain (FERM BP-
No. 5926) was inoculated with 100 mL of the preculture liquid and cultured at 30 ° C., 700 rpm, and an aeration rate of 3 l / min for 3 days. However, after the start of culturing, when the residual glucose concentration in the culture solution became 10 g / liter or less, glucose was sequentially added so that the residual glucose concentration became 50 g / liter. As for ammonium sulfate, 1/10 times the amount (weight ratio) of glucose was sequentially added simultaneously with the addition of glucose. For pH adjustment, the pH was maintained at 4 with 10% aqueous ammonia.
The residual glucose concentration at the end of fermentation after 3 days of culture was 20 g / liter. This fermentation broth was stored at room temperature for 24 hours while performing only aeration. Table 1 shows the εPL concentration and glucose concentration at the end of fermentation and after storage for 24 hours.
【００１８】実施例２
実施例１に準拠して、ストレプトマイセス・アルブラス
・サブスピーシズ・リジノポリメラス（Streptomyces a
lbulus subsp．lysinopolymerus）B21021株（FERM BP-
5926号）の前培養液100mLを培地に接種して培養を行っ
た。培養3日後の発酵終了時点でのグルコース濃度は5g/
リットルであった。該発酵液の保存中はグルコースを添
加することにより、グルコース濃度を常に3〜5g/リット
ルに維持した。発酵終了時及び24時間保存後のεＰＬ濃
度とグルコース濃度を表１に示した。Example 2 In accordance with Example 1, Streptomyces a.
lbulus subsp. lysinopolymerus) B21021 strain (FERM BP-
5926) preculture liquid (100 mL) was inoculated into the medium to carry out culture. The glucose concentration at the end of fermentation after 3 days of culture was 5 g /
It was liter. The glucose concentration was constantly maintained at 3 to 5 g / liter by adding glucose during storage of the fermentation broth. Table 1 shows the εPL concentration and glucose concentration at the end of fermentation and after storage for 24 hours.
【００１９】比較例１
実施例１に準拠して、ストレプトマイセス・アルブラス
・サブスピーシズ・リジノポリメラス（Streptomyces a
lbulus subsp．lysinopolymerus）B21021株（FERM BP-
5926号）の前培養液100mLを培地に接種して培養を行っ
た。培養3日後の発酵了時点でのグルコース濃度は５g／
リットルであった。該発酵液の保存中はグルコースの添
加はしなかった。発酵終了時及び24時間保存後のεＰＬ
濃度とグルコース濃度を表１に示した。COMPARATIVE EXAMPLE 1 In accordance with Example 1, Streptomyces ablus subspecies lysinopolymeras (Streptomyces a)
lbulus subsp. lysinopolymerus) B21021 strain (FERM BP-
5926) preculture liquid (100 mL) was inoculated into the medium to carry out culture. Glucose concentration at the end of fermentation after 3 days of culture is 5 g /
It was liter. Glucose was not added during storage of the fermentation broth. ΕPL at the end of fermentation and after storage for 24 hours
The concentration and glucose concentration are shown in Table 1.
【００２０】表１の実施例１，２から明らかなように、
発酵終了後も発酵液中にグルコースを常に残存させるこ
とにより、εＰＬ量の低下が抑制され、安定的に該εＰ
Ｌを採取することができることがわかる。As is clear from Examples 1 and 2 in Table 1,
By allowing glucose to always remain in the fermentation broth even after the end of fermentation, a decrease in the amount of εPL is suppressed and the εP is stably maintained.
It can be seen that L can be collected.
【００２１】[0021]
【表１】 注：表中、Ｌはリットルを表す。[Table 1] Note: In the table, L represents liter.
【００２２】[0022]
【発明の効果】本発明の製造法によれば、εＰＬを安定
的に採取することができることから、生産量を増大させ
ることが可能となり、収率よくεＰＬを製造することが
できる。According to the production method of the present invention, since εPL can be stably collected, the production amount can be increased and εPL can be produced in good yield.
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Claims (5)
レプトマイセス（Streptomyces）属微生物を好気的に培
地に培養して、ε―ポリ−Ｌ−リジンを採取する方法に
おいて、発酵終了後に発酵上澄み液と菌体を分離させる
までの間、常に発酵終了後の発酵液中に炭素源を残存さ
せておくことを特徴とするε―ポリ−Ｌ−リジンの製造
法。1. A method of collecting ε-poly-L-lysine by aerobically culturing a microorganism of the genus Streptomyces, which fermentatively produces ε-poly-L-lysine, in a medium to obtain fermentation. A method for producing ε-poly-L-lysine, characterized in that a carbon source is always left in the fermentation broth after the fermentation until the fermentation supernatant and the bacterial cells are separated later.
が1g/リットル〜100g/リットルである請求項１項記載の
ε―ポリ−Ｌ−リジンの製造法。2. The method for producing ε-poly-L-lysine according to claim 1, wherein the residual concentration of the carbon source in the fermentation liquid after the fermentation is 1 g / liter to 100 g / liter.
が２g/リットル〜２0g/リットルである請求項１記載の
ε―ポリ−Ｌ−リジンの製造法。3. The method for producing ε-poly-L-lysine according to claim 1, wherein the residual concentration of the carbon source in the fermented liquid after the fermentation is 2 g / liter to 20 g / liter.
コース、フラクトース、グリセリン、スターチ、ガラク
トース、マンニトール、イノシトール、サリシンの中か
ら選ばれた１種以上である請求項１〜３のいずれか１項
記載のε―ポリ−Ｌ−リジンの製造法。記載のε―ポリ
−Ｌ−リジンの製造法。4. The carbon source to be left in the fermentation broth is one or more selected from glucose, fructose, glycerin, starch, galactose, mannitol, inositol and salicin. The method for producing ε-poly-L-lysine according to item 1. Process for producing ε-poly-L-lysine as described.
レプトマイセス（Streptomyces）属微生物が、ストレプ
トマイセス・アルブラス・リジノポリメラス(Streptomy
ces albulus subsp. lysinopolymerus)B21021株（FERM
BP−5926）である請求項１記載のε―ポリ−Ｌ−リジ
ンの製造法。5. A microorganism belonging to the genus Streptomyces, which fermentatively produces ε-poly-L-lysine, is Streptomyces alblas lysinopolymerase (Streptomy).
ces albulus subsp.lysinopolymerus) B21021 strain (FERM
BP-5926), The method for producing ε-poly-L-lysine according to claim 1.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108796004A (en) * | 2018-06-20 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of technique of preparing lysine through fermentation |
| CN110373439A (en) * | 2019-08-01 | 2019-10-25 | 浙江新银象生物工程有限公司 | A method of stablizing quickly production epsilon-polylysine |
| CN110804572A (en) * | 2019-12-04 | 2020-02-18 | 江南大学 | Streptomyces and method for preparing epsilon-polylysine by using same |
| CN112852668A (en) * | 2021-01-21 | 2021-05-28 | 齐鲁工业大学 | Acid-resistant streptomyces albidoflavus and application thereof in epsilon-polylysine fermentation |
-
2001
- 2001-12-21 JP JP2001390102A patent/JP3915505B2/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108796004A (en) * | 2018-06-20 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of technique of preparing lysine through fermentation |
| CN110373439A (en) * | 2019-08-01 | 2019-10-25 | 浙江新银象生物工程有限公司 | A method of stablizing quickly production epsilon-polylysine |
| CN110373439B (en) * | 2019-08-01 | 2021-04-27 | 浙江新银象生物工程有限公司 | Method for stably and rapidly producing epsilon-polylysine |
| CN110804572A (en) * | 2019-12-04 | 2020-02-18 | 江南大学 | Streptomyces and method for preparing epsilon-polylysine by using same |
| CN112852668A (en) * | 2021-01-21 | 2021-05-28 | 齐鲁工业大学 | Acid-resistant streptomyces albidoflavus and application thereof in epsilon-polylysine fermentation |
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