JPH09143024A - Composition for external rpeparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition for skin cosmetics which contains a natural high-molecular substance as a combination of specific two kinds of acidic heteropolysaccharides, shows excellent skin moisture retention, skin protecting effect, flexibility and flattening effects, and excellent application feeling and stability. SOLUTION: An acidic heteropolysaccharide obtained from a plant in Polyanthus, particularly tuberose (Polianthes tuberosa) is combined with another heteropolysaccharide obtained from a kind of mushroom growing on decaying wood (Jew's ear) (Tremella fuciformis Berk) preferably from its fruiting body. Their formulations are preferably 0.0001-10wt.%, particularly 0.001-5wt.%, respectively. The acidic polysaccharide originating from tuberose is preferably obtained, for example, by using a part of tuberose flower as an explant to induce culls with a culture medium prepared by adding a plant hormone and a saccharide as a carbon source to the basic culture medium, transferring the culls to a liquid culture medium and culturing them, namely by tissue culture of tuberose. The acidic hetero polysaccharide is preferably obtained by extraction of its fruiting bodies obtained by liquid culture of its mycelium.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition for external use, and more particularly to a skin moisturizing effect, a protective effect, a softening / softening effect.
The present invention relates to an external preparation composition such as a skin cosmetic, a hair cosmetic, and an external drug, which are excellent in effects such as smoothing, feeling in use and stability.

[0002]

2. Description of the Related Art Conventionally, low molecular weight substances such as glycerin and sorbitol, NMF (natural moisturizing factor) components such as sodium pyrrolidonecarboxylate have been used as external preparations, especially moisturizers for cosmetics. , Biopolymers such as mucopolysaccharides and various plant extracts are used as moisturizers. However, as is apparent from the fact that plant-derived polysaccharides are used as thickeners, gelling agents, etc., if the amount used is sufficient to exert a moisturizing function, the system thickens and Feels bad,
In addition, natural polymer materials have drawbacks such as stability problems.

[0003]

SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide an external preparation composition containing a natural polymer substance which is excellent in moisturizing effect, feeling of use and stability.

[0004]

[Means for Solving the Problems] Under such circumstances, the present inventor has conducted diligent studies, and as a result, externally added a combination of an acidic heteropolysaccharide derived from a Polyanthes plant and an acidic heteropolysaccharide derived from a white jellyfish. The agent was found to be excellent in moisturizing effect, feeling in use, stability and the like, and completed the present invention.

That is, the present invention relates to the genus Polyantes (Po
The present invention provides an external preparation composition containing an acidic heteropolysaccharide obtained from a plant belonging to Lianthes L.) and an acidic heteropolysaccharide obtained from a white jellyfish (Tremella fuciformis Berk).

[0006]

Best Mode for Carrying Out the Invention Polyanthes (Polianthes L.) for obtaining acidic heteropolysaccharides used in the present invention
The plant belonging to is tuberose (Polianthes tub
erosa L.), and the tuberose-derived acidic heteropolysaccharide can be obtained, for example, by the following method.

Tissue culture method: Callus is induced by using a part of a tuberose flower or the like as an explant and using a medium in which a plant hormone and sugar as a carbon source are added to a basic medium. The callus was further transferred to a liquid medium and shake-cultured, and the cells were removed from the culture by centrifugation or filtration, and then concentrated, and ethanol was added to the concentrated solution to precipitate, and the precipitate was freeze-dried. Obtain an acidic heteropolysaccharide.

In the present method, a part of organs or tissues such as flowers, stems, leaves, bulbs and roots of tuberose is used as an explant, and a part of flowers is particularly preferable.

As a basal medium for inducing callus, Murasige-Skoog medium, Linsma, which is commonly used for plant tissue culture, is used.
ier-Skoog's medium, Gamborg's medium, White's medium, Tulec
Ke's medium, Nitsch & Nitsch medium, etc. can be used.

It is necessary to add a plant hormone to this basic medium, and as the plant hormone, 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthalene acetic acid (NAA), indole acetic acid (IAA) is used. , Auxins such as indolebutyric acid (IBA); cytokinins such as furfurylaminopurine (kinetin), benzyladenine (BA), and dimethylaminopurine (2iP). Among them, 2,4-D alone or NAA
The combination of BA with BA or the combination of NAA with kinetin gives good results. The plant hormone concentration required for callus induction is 5 × 10 ⁻⁴ M to 1 × 1 in the case of 2,4-D alone.
In the case of a combination of 0 ⁻⁷ M, NAA and BA or NAA and kinetin, the concentration of NAA is 5 × 10 ⁻⁴ M to 1 × 10 ^−7.
The concentration of M, BA or kinetin is 1 × 10 ⁻⁴ M to 1 ×
It is 10 ^-7 M.

In addition to the above basic medium and plant hormones, sugar is added to the callus induction medium as a carbon source. Examples of the sugar include glucose, fructose, mannose, xylose, saccharose, rhamnose, fucose, starch and the like, and usually saccharose is used.

Callus induction can be carried out in either a solid medium or a liquid medium, but a solid medium is usually used.

The induced callus can be subcultured for 10 or more generations while maintaining the same morphology in the above callus induction medium. As a medium for subculturing, usually, Linsmaier-Skoog's medium as a basic medium, Murasige-Sk
oog medium, 1 × 10 ^-4 M to 1 × 1 as plant hormone
0 ⁻⁷ M 2,4-D or 1 × 10 ⁻⁴ M to 1 × 10 ⁻⁷ M
NAA and 1 × 10 ⁻⁴ M to 1 × 10 ⁻⁷ M BA, and as the carbon source, glucose, fructose, mannose, xylose, sucrose, rhamnose, fucose, starch and the like are used. Among them, sucrose is The addition amount is preferably 1 to 6%.

To produce a polysaccharide from callus, callus is cultured in a solid medium such as an agar medium or a liquid medium, and it is particularly preferable to culture in a liquid medium.

The basal medium is the same as the callus induction medium, for example, Murasige-Skoog medium, Linsmaier-Skoog medium.
Medium, Gamborg medium, White medium, Tulleke medium, Nitsch & Nitsch medium and the like can be used, and among them, Murasige-Skoog medium and Linsmaier-Skoog medium are preferable.

The type and concentration of plant hormones are related to the productivity of polysaccharides such as 2,4-D, NAA and IA.
Auxins such as A and IBA; kinetin, BA, 2i
Cytokinins such as P; gibberellins such as gibberellin A ₃ (GA ₃ ) and the like are used. Among them, 2,4-D,
It is preferred to use NAA alone or in combination with NAA and BA or kinetin. Its concentration is 2,4
When -D or NAA is used alone, it is 5 × 10 ^-4 M
1 × 10 ⁻⁷ M, especially 1 × 10 ⁻⁴ M to 5 × 10 ⁻⁶ M; N
When AA and BA or NAA and kinetin are used in combination, the concentration of NAA is 1 × 10 ⁻⁴ M to 1 × 10.
^-7 M, especially 1 x 10 ^-4 M to 5 x 10 ^-6 M, and the concentration of BA or kinetin is 5 x 10 ^-5 M to 1 x 10 ^-9 M, especially 1
× 10 ^-5 M to 1 × 10 ^-7 M is preferable.

As the carbon source, glucose, fructose, mannose, xylose, sucrose, rhamnose, fucose, starch and the like are used. Polysaccharide production is not so strongly affected by the type of carbon source added, and saccharose is usually used. Although there is not a deep relationship between the concentration of the carbon source and the production amount of the polysaccharide, generally, 1 to 6% is preferable.

The culture method is not particularly limited, but usually 20
It is preferably carried out at a temperature of -30 ° C for 15-30 days, and shaking culture is preferred.

To collect the polysaccharide from the thus obtained culture, for example, cells are removed from the culture by centrifugation or filtration, and then the culture solution is concentrated using a rotary evaporator or the like and concentrated. It is carried out by adding ethanol to the liquid to cause precipitation, and freeze-drying the precipitate.

White jellyfish (Tremella f) used in the present invention
The acidic heteropolysaccharide derived from uciformis Berk) can be obtained, for example, by the following method.

Extraction from fruiting body: The dried white jellyfish is washed with water, water is added to this to perform extraction, the solid matter is taken out and the same extraction is performed again to obtain an extract. This extract and the previously obtained extract are combined, centrifuged to obtain a supernatant, the supernatant is concentrated, ethanol or the like is added thereto to cause precipitation, and the precipitate is dried. Obtain an acidic heteropolysaccharide.

Liquid culture of bacterial cells: The liquid culture conditions of the Pleurotus cornucopiae cells are not particularly limited as long as the conditions are such that the bacterial cells of the Pseudomonas aeruginosa can grow. Usually, as a medium for liquid culture, a medium containing a carbon source, a nitrogen source, an inorganic salt can be used, and in addition, a medium supplemented with natural nutrient sources, vitamins, amino acids and the like can also be used. Examples of the carbon source include the same sugars as described above. Examples of the carbon source include peptone, urea, ammonium salt and the like. Moreover, examples of the inorganic salt include potassium chloride, iron sulfate, magnesium sulfate, and phosphate, and these can be added as necessary. Examples of natural nutrient sources include malt extract, yeast extract, casein hydrolyzate, coconut milk, potato extract and the like.

The culture conditions are a temperature of 20 to 32 ° C. and a pH of 5 to 7.
It is preferable to carry out under aerobic conditions for 5 to 20 days by a method such as shaking culture, aeration and stirring culture. Although the main culture can be carried out from the beginning, it is preferable to carry out a small-scale pre-culture in advance and inoculate this to the main culture.

The liquid culture after the liquid culture may be sterilized by an ordinary method, heat-sterilized, filtered, and used as it is, or further fractionated and purified. Further, the above culture broth and the fractionated / purified product can be used after being concentrated or diluted as necessary, and can be further dried by a method such as freeze-drying or spray-drying to be used as a dry powder.

The content of the acidic heteropolysaccharide in the external preparation composition of the present invention is 0.0001 to 10% by weight (from below, respectively) derived from Polyanthes plant and Ganoderma lucidum from the viewpoint of exerting sufficient effect. It is preferable that the amount is simply indicated by%), and it is particularly preferable that the amount is 0.001 to 5%.

The external preparation composition of the present invention is roughly classified into a medicated external preparation for skin and a cosmetic composition in its use form.

Examples of the external medicated skin agent include various ointments containing medicinal components. The ointment may be any of those based on an oily base and those based on an oil / water or water / oil type emulsified base. The medicinal component is not particularly limited and, for example, an analgesic / anti-inflammatory agent, an antipruritic agent, a bactericidal disinfectant, an astringent agent, an emollient agent, a hormone agent and the like can be appropriately used as necessary.

As the cosmetics, various forms such as water / oil, oil / water emulsion cosmetics, creams, cosmetic emulsions,
Skin cosmetics such as lotion, oily cosmetics, lipsticks, foundations, hair nicks, hair styling agents, hair nourishing agents and hair restorers can be used.

Oily components that are preferably used in the preparation of these external preparations include, for example, hydrocarbons such as liquid paraffin, paraffin wax, ceresin and squalane;
Waxes such as beeswax, spermaceti, and carnauba wax; natural animal and vegetable oils and fats such as olive oil, camellia oil, jojoba oil, and lanolin; silicone oils, fatty acids, higher alcohols, and ester oils obtained by reacting these.

As the surfactant, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester,
Polyoxyethylene hydrogenated castor oil alkyl sulfate, polyoxyethylene alkyl sulfate, alkyl phosphate, polyoxyethylene alkyl phosphate, fatty acid alkali metal salt, sorbitan fatty acid ester, glycerin fatty acid ester and the like are used. Further, the emulsion composition of the present invention may further contain various optional components, for example, a polymer compound such as polyvinyl alcohol, carboxyvinyl polymer, carboxymethylcellulose, polyvinylpyrrolidone, hydroxyethylcellulose, or methylcellulose as a viscosity modifier;
Natural gums such as gelatin and taracant gum; alcohols such as ethanol and isopropanol; humectants such as propylene glycol, glycerin, 1,3-butylene glycol, dipropylene glycol, sorbitol, milky lotion, sodium lactate and sodium pyrrolidonecarboxylate. However, examples of the preservative include paraoxybenzoic acid ester, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, phenoxyethanol and the like.

[0031]

The external preparation composition of the present invention is excellent in skin moisturizing effect, protective effect and stability by blending the tuberose-derived acidic heteropolysaccharide and the white jellyfish-derived acidic heteropolysaccharide, and is smooth. It has a good usability.

[0032]

The present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

Production Example 1 Production of Regenerated Heteropolysaccharide Derived from Tuberose: Flowering 2
-7 days before, the buds of the tuberose were cut out, sterilized with 70% ethanol and 1% sodium hypochlorite, washed with sterilized water, cut to an appropriate size and placed on a callus induction medium as an explant.

As a callus induction medium, a Linsmaier-Skoog medium containing 0.8% agar was used, and α-naphthylacetic acid 1 × 10 ^-5 M and benzyladenine 1 × 10 ^-6 M were used as plant hormones, and a carbon source was used. 3% sucrose was added. Culturing was carried out at 25 ± 2 ° C for 30 days.
Callus was induced from each explant after a day.

These calli were separated from the explants and cultured under the same conditions using a medium having the same composition as the callus induction medium. Callus was repeatedly transplanted to a new medium every 30 days for growth, and then shaking culture was performed using a liquid medium containing the same components as the above-mentioned callus induction medium but containing no agar. The amount of medium is 8 per 200 ml Erlenmeyer flask.
Make up 0 ml and inoculate 2 g of callus with a fresh weight of 25 ± 2
The culture was carried out with shaking at 120 ° C. for 30 days.

The cells were removed from 1 L of this culture medium by centrifugation, and the culture medium was concentrated using a rotary evaporator. To 250 ml of the concentrated culture solution, 750 ml of ethanol was added, and the mixture was stood at 5 ° C. for 24 hours to obtain a precipitate.

The precipitate was recovered by centrifugation and 70%
After washing with ethanol, the water was removed by freeze-drying to obtain 2.9 g of tuberose-derived polysaccharide. The components constituting the polysaccharide were arabinose, galactose, glucose, mannose and xylose, and glucuronic acid as an acidic sugar, which was an acidic heteropolysaccharide.

Production Example 2 Production of Acid Polysaccharide Derived from Saccharomyces cerevisiae 50 g of dried Saccharomyces communis was washed with cold water to prepare 5 L of water.
Was added and extraction was carried out at 100 ° C. for 1 hour. The solid matter was removed using gauze to obtain about 2 L of extract. The above solid matter was subjected to the same extraction again, and the solid matter was removed using gauze to obtain about 2 L of an extract. The extract was combined with the first extract and then centrifuged to obtain about 3.5 L of transparent supernatant.

Next, this supernatant was concentrated to about 500 ml using a rotary evaporator, 1.5 L of ethanol was added to the concentrate, and the resulting precipitate was separated by filtration. The precipitate was freeze-dried to obtain 4.2 g of a white jellyfish polysaccharide. The components constituting the polysaccharide were glucose, mannose and xylose, and glucuronic acid as an acidic sugar, which was an acidic heteropolysaccharide.

Example 1 A lotion was prepared by the following formulation and manufacturing method, and its moisturizing effect and feeling of use were examined.

[0041]

[Table 1]

(Production method) In purified water, (1), (2),
(3), (5), and (6) were dissolved by heating and returned to room temperature, then, those in which (4), (8), and (9) were dissolved were slowly added to solubilize and filtered. I got lotion.

Test Example 1 Using the lotion obtained in Example 1, a moisturizing effect test was conducted. That is, 0.02 ml / 4 cm ^{2 of the} product of the present invention was applied to the forearm of a healthy person, and the water content of the stratum corneum was measured after 1 hour (n = 5, measuring device: SKICON- manufactured by IBS).
200). The results are shown in the following table.

[0044]

[Table 2]

The water content at the application site of the product of the present invention was clearly higher than that at the application site of the comparative product, and it was confirmed that the lotion containing the tuberose-derived polysaccharide and the white jellyfish-derived polysaccharide had a high moisturizing effect. .

Test Example 2 With respect to 20 women aged 20 to 35, the use test of the product of the present invention and the comparative product (compare evaluation) was carried out in terms of smoothness during use and its durability, squeaky, sticky and moist feeling.
Was done. The results are shown in the following table.

[0047]

[Table 3]

The lotion containing the tuberose-derived polysaccharide and the white jellyfish-derived polysaccharide has a good feeling of smoothness during use, high durability, low stickiness and relatively low squeaky feeling. there were.

Front Page Continuation (72) Inventor Seiji Yamazaki, 2-1-3 Fumika, Sumida-ku, Tokyo Inside Kao Institute of Stock Companies (72) Inventor Kaori Ikawa 2606, Akabane, Kaicho, Haga-gun, Tochigi Prefecture Inside Kao Institute of Stock Companies

Claims (4)

[Claims] 1. An external preparation composition containing an acidic heteropolysaccharide obtained from a plant belonging to the genus Polyanthes (Polianthes L.) and an acidic heteropolysaccharide obtained from Tremella fuciformis Berk. 2. The external preparation composition according to claim 1, wherein the plant belonging to the genus Polyantes is tuberose (Polianthestuberosa L.), and the acidic heteropolysaccharide is obtained by tissue culture of tuberose. 3. The external preparation composition according to claim 1, wherein the acidic heteropolysaccharide obtained from Pleurotus cornucopiae is obtained by extraction from fruiting bodies or liquid culture of cells. 4. The content of the acidic heteropolysaccharide obtained from a plant belonging to the genus Polyanthes and the amount of the acidic heteropolysaccharide obtained from a white jellyfish are 0.0001 to 1 respectively.
The external preparation composition according to any one of claims 1 to 3, which is 0% by weight.