JPH0870721A - Disease injury-controlling agent for cultured laver and control of disease injury of cultured laver - Google Patents

Abstract

PURPOSE: To obtain a disease injury-controlling agent capable of controlling attached germs such as Olpidiopsis or Pyshium propagating during laver culture by the germicidal potency of the contained acid itself without damaging laver and also without causing marine environmental pollution, by mixing an aqueous formic acid solution with specific acid(s) such as phytic acid. CONSTITUTION: This disease injury-controlling agent is obtained by mixing a 0.3w/v% aqueous formic acid solution (part or the whole of the dispersion medium may be seawater) with an aqueous solution of at least one kind of acid selected from phytic acid, lactic acid, acetic acid, adipic acid, succinic acid, malic acid, citric acid, maleic acid, fumaric acid, gluconic acid, tartaric acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, glutaric acid, ketoglutaric acid, phosphoric acid, hydrochloric acid, sulfuric acid and nitric acid. In this case, the respective concentrations of these acids (except the formic acid) are set at 0.01-3w/v% correspondingly to the time necessary for treating object laver. Object cultured laver is immersed in the resultant mixed solution.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a treatment agent for exterminating adherent bacteria such as red rot fungus, jar-like fungus and the like, which occur during seaweed culture, and a method for exterminating disease in cultured seaweed.

[0002]

2. Description of the Related Art In seaweed cultivation, when a red rot disease occurs, the quality of seaweed is deteriorated, and when it becomes severe, production may be completely lost. Generally, in seaweed aquaculture, a draining operation is performed as a method for preventing and controlling disease. This is a method of removing the seaweed net from the seawater once and drying it, but this method is not very effective even though it takes a lot of labor and time.

As another method, there is also a method of exterminating red-rot fungi by immersion treatment in an organic acid solution such as citric acid or malic acid.

Nori producers average 100 to 200
The seaweed nets are collected in one sheet, and the shorter the treatment time, the better the workability. For example, if the treatment is shortened by 5 minutes, the time can be saved from 500 minutes to 1000 minutes, and thus more laver net can be treated. However, being able to exterminate red-rot fungi in a short time inevitably means that it is a severe drug against seaweed itself and damages the seaweed.

When it comes to short-time treatment, in recent years, it has been called a muzzle boat to carry out treatment while partially passing the seaweed net over the ship, but in this case, the treatment chemical naturally flows into the sea. Will be done. This poses a problem of environmental pollution because otherwise the treatment chemicals will flow into the sea.

Therefore, without harming the seaweed itself,
It is possible to exterminate adherent bacteria such as red rot bacteria and jars in a short time.
Moreover, various chemicals have been proposed, which require chemicals that can be removed by a dilute solution as much as possible, and that do not harm other fish and shellfish even if the waste solution flows into the sea and that does not cause environmental pollution.

In Japanese Patent Publication No. 46-35873, P-
There has been proposed a method for preventing red rot of Porphyra angustifolia using oxybenzoic acid or its ester. JP-A-50-
No. 1214725 discloses an organic carboxylic acid selected from a saturated fatty acid carboxylic acid having 1 to 4 carbon atoms, a saturated or unsaturated carboxylic acid having 2 to 4 carbon atoms, glycolic acid, lactic acid, tartaric acid, malic acid and citric acid. Species or algaecide containing two or more species is disclosed. Although this document exclusively describes the action of germicidal algae, there is no description of red-rot fungi.

Japanese Patent Publication No. 60-13647 discloses that it contains citric acid in an amount of 0.3 to 5.0% by weight and has a pH of 1.0 to 6.
Nori seaweed cultivating method by controlling and exterminating algae and diseases that are immersed in the treatment liquid of No. 0 is described. Japanese Examined Japanese Patent Sho 60-13648
The publication describes a seaweed aquaculture method by adding weeds such as hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid and the like to adjust pH to 1.0 to 4.0 and controlling disease and disease.

Japanese Patent Publication No. 60-21950 discloses a fertilizer for seaweed cultivation containing phytic acid or a salt thereof as an active ingredient, a preventive effect against red rot, and a diatom control effect.
Japanese Unexamined Patent Publication No. 57-8722 discloses a seaweed aquaculture method in which the seaweed net is treated with a treatment solution containing phosphoric acid or a salt thereof to remove and prevent diseases such as weeds and red rot. There is.

Japanese Unexamined Patent Publication (Kokai) No. 60-87202 discloses a fungicide for edible seaweed seaweed bacteria containing adipic acid as an active ingredient. Japanese Unexamined Patent Publication No. 60-244245 discloses a method of algicidal treatment in which a fumaric acid in a saturated amount or more is present, and one or more kinds of malic acid, citric acid, tartaric acid, and succinic acid may be used in combination. There is also a description.

Japanese Unexamined Patent Publication (Kokai) No. 60-248121 discloses that weeds and acupuncture fungi by immersing in a treatment solution containing one or more of halogenated carboxylic acids such as chloroacetic acid, dichloroacetic acid and trichloroacetic acid. There is disclosed a seaweed aquaculture method for controlling weeds, acupuncture fungus diseases, reddening diseases and the like by immersing in a seaweed culture solution for controlling the disease, reddening diseases and the like.

Japanese Unexamined Patent Publication (Kokai) No. 62-21784 discloses a liquid fertilizer that also serves as an algicidal agent for aquaculture, which is composed of an acidic buffer solution containing dilute hydrochloric acid as a main component, a nitrate salt, an ammonium salt and a phosphate salt.

Japanese Unexamined Patent Publication (Kokai) No. 62-190103 discloses the genus Pythium and Olpidiops, which are the pathogens of red rot and acupuncture fungus having gluconic acid as an active ingredient.
Disinfectants for algae of the genus is are disclosed. JP-A-1-
Japanese Patent No. 279805 discloses a repellent for algae and bacteria containing monosodium fumarate and / or monopotassium fumarate as an active ingredient.

Japanese Unexamined Patent Publication (Kokai) No. 2-25404 discloses a pesticide containing fumaric acid and / or fumarate and at least one selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, potassium carbonate and potassium hydrogen carbonate. Disinfectants for algae and bacteria which are dissolved in water or seawater to form monosodium fumarate and / or monopotassium fumarate are disclosed.

JP-A-2-291218 (Japanese Patent Publication No.
(JP-A-47810), there is disclosed an algae killing method in which fumaric acid in an amount exceeding the saturated amount is allowed to exist in a state of being covered with a coating means having water permeability, and an object to be treated is dipped in the treatment liquid. . JP-A-5-139913 discloses an algicidal agent containing 0.1 to 2.0% by weight of lactic acid and a pH adjuster and adjusting the pH to 1.5 to 2.0. . Further, in this specification, it is described that it is also effective for controlling red rot fungus.

[0016]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a drug and a disease control method capable of controlling adherent bacteria such as red rot fungus and jar fungus in a short time without damaging the seaweed itself. Is.

[0017]

As a result of intensive studies for solving the above-mentioned problems, the present inventor has found that an aqueous solution of formic acid has a high degree of damage to seaweed, a certain concentration or more, and a certain treatment time. Above, the seaweed is killed, while on the other hand, it has not been used at all since it has no effect against the red-rot fungus and cannot be used as a red-rot fung control agent at a concentration below the treatment time. However, the inventors of the present invention have completed the present invention by discovering that if this formic acid is used in combination with phytic acid, lactic acid, phosphoric acid, etc., the bactericidal activity of the acid itself is significantly increased, and therefore, it can be treated at a low concentration for a short time and environmental pollution can be reduced.

That is, the present invention is as follows. (1) In an aqueous solution of formic acid, phytic acid, lactic acid, acetic acid, adipic acid, succinic acid, malic acid, citric acid, maleic acid, fumaric acid, gluconic acid, tartaric acid, monochloroacetic acid,
A disease control agent for cultured seaweed, comprising at least one selected from the group consisting of dichloroacetic acid, trichloroacetic acid, itaconic acid, glutaric acid, ketoglutaric acid, phosphoric acid, hydrochloric acid, sulfuric acid, and nitric acid.

(2) Formic acid aqueous solution (including part or all of seawater) concentration of 0.3 W / V% or less, phytic acid,
Lactic acid, acetic acid, adipic acid, succinic acid, malic acid, citric acid, maleic acid, fumaric acid, gluconic acid, tartaric acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, glutaric acid, ketoglutaric acid, phosphoric acid, hydrochloric acid, At least one acid selected from the group consisting of sulfuric acid and nitric acid is set in the concentration range of 0.01 to 0.3 W / V% in the aqueous solution according to the required treatment time, and the cultured seaweed is immersed in this mixed solution. Or a method for exterminating the disease of cultured seaweed, which comprises spraying this solution onto the cultured seaweed.

(3) Phosphoric acid, fumaric acid, ketoglutaric acid, lactic acid, sulfuric acid, nitric acid, acetic acid, formic acid aqueous solution (including part or all of seawater) at a concentration of 0.01 to 0.1 W / V%, Concentration of at least one acid selected from the group consisting of glutaric acid, tartaric acid, itaconic acid, adipic acid, succinic acid, citric acid, gluconic acid, maleic acid, malic acid, phytic acid, and hydrochloric acid in the aqueous solution of 0.05 to 0 A short-term disease control method for cultured seaweed characterized by immersing the cultured seaweed in this mixed solution or spraying this solution on the cultured seaweed by setting the range to 3 W / V% corresponding to the required treatment time. .

As can be seen from Test Example 1, since formic acid has a high degree of damage to seaweed, the seaweed is damaged and killed before the red rot fungus is exterminated. Therefore, formic acid cannot be used alone as a repellent for red-rot fungi. However, as shown in Test Example 2 and later, it was found that by combining with other acids such as phytic acid, lactic acid and acetic acid, seaweed is not damaged and the bactericidal power of the combined acid itself can be considerably increased. It was Currently, there is a fishing ground where a mog boat has been developed and is performing short-time treatment (treatment within 1 minute). By using formic acid and phytic acid, lactic acid, acetic acid, etc. together, it can be used at a low concentration. The amount of acid used can be reduced. In the case of processing with a muzzle boat, if an inorganic acid such as hydrochloric acid or phosphoric acid is used, a concentration of 2 to 8% is required. Therefore, by using the present invention, the amount of acid used can be considerably reduced, and the environmental pollution load on the ocean can be reduced.

Further, as can be seen from the adherent bacterial extermination test of Test Example 10, when treated with formic acid alone, the extermination effect is small, and if the concentration is increased to enhance the effect, the seaweed will be killed. Treatment with the treatment liquid of the present invention in which lactic acid, acetic acid and the like are used in combination with formic acid can considerably increase the bactericidal power for exterminating adherent bacteria without damaging the seaweed.

The pesticide of the present invention comprises ammonium nitrate, ammonium chloride, monoammonium phosphate as a nitrogen source,
Urea, sodium nitrate, nitric acid and the like can be used in combination with 1 potassium phosphate, 1 sodium phosphate, inositol 6-phosphate phosphate and phosphoric acid as a phosphorus source. Particularly, when phosphoric acid and phosphate are used together, the damage of seaweed can be alleviated and soft seaweed can be produced.

The pesticide of the present invention is used by a method of immersing a seaweed culture net infected with red rot fungus in the pesticide or by spraying the pesticide on the culture net. In this way, by contacting the nori leaf body infected with the disease with the pesticide,
It does not damage seaweed and selectively removes disease-causing bacteria, adherent bacteria, diatoms, etc. Treatment of aquaculture nets that do not contain disease is also carried out as a preventive measure.

[0025]

EXAMPLES The present invention will be described in more detail with reference to examples and comparative examples, but the present invention is not limited to these examples. In the following test examples,
The mark indicates that there is no disorder of laver and the red rot fungus is completely eradicated, and the symbol indicates that there is no disorder of laver and red rot is considerably suppressed, both of which are examples. is there.
Other marks are comparative examples.

(Test Example 1) 0.00 of formic acid (88%)
5, 0.01, 0.05, 0.1, 0.2, 0.3,
Prepare a 0.5 W / V% seawater solution for a certain period of time (5 seconds, 1
0 seconds, 20 seconds, ... 60 seconds, 2 minutes, 3 minutes, 4 minutes, ... 10 minutes) After dipping the red rot infected seaweed, it was washed with seawater and the state after 2 days of static culture under a microscope It was observed and the effect of exterminating red rot fungus and damage to seaweed was investigated. The results are shown in Table 1.

[0027]

[Table 1]

0.005% and 0.01% of formic acid is 10
The red-rot fungus cannot be exterminated at all even by the immersion treatment for minutes. If it is 0.05% or 0.1%, it cannot be used as a repellent for red rot fungi because it has no effect of repelling red rot fungus or is damaged. If it is 0.2% or more, it cannot be used at all because it is damaged for 5 seconds. From the above, formic acid alone cannot be used as a repellent for red-rot fungi.

(Test Example 2) 0.005 of formic acid (88%)
Ketoglutaric acid, maleic acid, phosphoric acid (75%), fumaric acid, malic acid, lactic acid (90%), and hydrochloric acid (36%) were added to a W / V% seawater solution at 0.01, 0.05, and 0. 1,
To the adjustment solution added to 0.2, 0.3, W / V%,
Nori leaves infected with red rot fungus for 1 minute, 2 minutes, 3 minutes, ...
After the immersion treatment for 10 minutes, it was washed with seawater and the state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot bacteria. The evaluation is the same as in Test Example 1. The results are shown in Table 2.

[0030]

[Table 2]

Test Example 3 0.01 W of formic acid (88%)
Monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, sulfuric acid, nitric acid (61%), acetic acid (90%), glutaric acid, tartaric acid, itaconic acid, adipic acid, succinic acid, citric acid, fumaric acid, ketoglutar Acid, lactic acid (90
%), Gluconic acid, maleic acid, malic acid, phytic acid (50%), phosphoric acid (75%) and hydrochloric acid (36%) at 0.01, 0.05, 0.1, 0.2 and 0, respectively. 3W / V%
The seaweed leaf bodies infected with red rot fungus were immersed in the adjusted solution added as above for a certain period of time, then washed with sea water, and the state after 2 days of static culture was observed under a microscope to remove the red rot fungus. The effect was investigated. The evaluation is the same as in Test Example 1. The results are shown in Table 3, Table 4, Table 5, Table 6, Table 7, and Table 8.

[0032]

[Table 3]

[Table 4]

[Table 5]

[Table 6]

[Table 7]

[Table 8]

Test Example 4 Formic acid (88%) 0.05 W /
Phosphoric acid (75%), hydrochloric acid (36%), fumaric acid, malic acid, lactic acid (90%), ketoglutaric acid,
Maleic acid 0.01, 0.05, 0.1,
0.5, 1, 1.5, 2, 2,5,1,2,5 of seaweed thalli infected with red rot fungus was added to the regulator added to 0.2,0.3W / V%.
After dipping treatment for 2.5, 3, 3.5, 4, 4.5, 5 minutes, it was washed with sea water and the state after 2 days of static culture was observed under a microscope to examine the effect of controlling red rot bacteria. Evaluation is Test Example 1
Is the same as. The results are shown in Tables 9 and 10.

[0034]

[Table 9]

[Table 10]

(Test Example 5) 0.1 W / V% of formic acid (88%)
Monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, sulfuric acid, nitric acid (61%), acetic acid (90%), glutaric acid, tartaric acid, itaconic acid, adipic acid, succinic acid
Citric acid, fumaric acid, ketoglutaric acid, lactic acid (90
%), Gluconic acid, maleic acid, malic acid, phytic acid (50%), phosphoric acid (75%) and hydrochloric acid (36%) at 0.01, 0.05, 0.1, 0.2 and 0, respectively. 3W / V%
Was added to the prepared solution to give the red rot-infected nori leaf bodies for a certain period of time (5, 10, 20, 30, 40, 5).
(0, 60, 90, 120 seconds) After immersion treatment, it was washed with seawater, and the state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot bacteria. The evaluation is the same as in Test Example 1. The results are shown in Table 11, Table 12, Table 13, and Table 14.

[0036]

[Table 11]

[Table 12]

[Table 13]

[Table 14]

Test Example 6 0.2 W / V% of formic acid (88%)
Ketoglutaric acid, maleic acid, phosphoric acid (7
5%), fumaric acid, malic acid, lactic acid (90%), hydrochloric acid (36%) at 0.01, 0.05, 0.1, respectively.
0.2 and 0.3 W / V% were added. For the adjustment liquid,
Nori leaves infected with red rot bacteria for a certain period of time (5, 10, 2
(0, 30, 40, 50, 60 seconds), after immersion treatment, washed with seawater, and observed under microscope for 2 days after static culture,
The effect of controlling red rot fungus was investigated. The evaluation is the same as in Test Example 1. The results are shown in Tables 15 and 16.

[0038]

[Table 15]

[Table 16]

Test Example 7 0.3 W / V% of formic acid (88%)
Monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, sulfuric acid, nitric acid (61%), acetic acid (90%), glutaric acid, tartaric acid, itaconic acid, adipic acid, succinic acid
Citric acid, fumaric acid, ketoglutaric acid, lactic acid (90
%), Gluconic acid, maleic acid, malic acid, phytic acid (50%), phosphoric acid (75%) and hydrochloric acid (36%) at 0.01, 0.05, 0.1, 0.2 and 0, respectively. 3W / V%
The seaweed thalli infected with red rot fungus was immersed in the adjusted solution added so as to be constant time (5, 10, 20, 30 seconds), washed with seawater, and the state after 2 days of static culture was adjusted. It was observed under a microscope to investigate the effect of controlling red rot bacteria. The evaluation is the same as in Test Example 1. The results are shown in Table 17, Table 18, and Table 1.
9, shown in Table 20.

[0040]

[Table 17]

[Table 18]

[Table 19]

[Table 20]

(Test Example 8) For 10 seconds, 20 seconds, ... 60 seconds of red rot fungus-infected laver leaves were added to a seawater solution of the following combination in which two or more other acids were added to formic acid (88%). 1.5
Min, 2 min, 2.5 min, 3 min, 4 min, 4.5 min, 5 min, dip, wash with seawater, and observe the condition after 2 days of static culture under a microscope to eliminate red rot bacteria The effect was investigated. The combination formulations were A, B and C in Table 21 below. Table 22 shows the control effect of the red rot fungus.

[0042]

[Table 21]

[Table 22]

(Test Example 9) (Comparative Example) Monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, sulfuric acid, nitric acid (61%), acetic acid (90%), glutaric acid, tartaric acid, itaconic acid, adipic acid, succinic acid, citric acid. acid,
Fumaric acid, ketoglutaric acid, lactic acid (90%), gluconic acid, maleic acid, malic acid, phytic acid (50%), phosphoric acid (75%), hydrochloric acid (36%), 0.01, 0.
05, 0.1, 0.2, 0.3 W / V% seawater solution was prepared,
The seaweed lobes infected with red rot fungus were immersed for a certain period of time, washed with sea water, and the state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot fungus. The evaluation is the same as in Test Example 1. The results are shown in Table 23, Table 24, Table 25,
The results are shown in Table 26, Table 27, Table 28, and Table 29.

[0044]

[Table 23]

[Table 24]

[Table 25]

[Table 26]

[Table 27]

[Table 28]

[Table 29]

(Test Example 10) Various acids used in combination with formic acid in the present invention were 0.01%, 0.05% and 0.1%.
Formic acid concentration of 0% for 1%, 0.2% and 0.3%,
0.005%, 0.01%, 0.05%, 0.1%,
Table 30, Table 31, Table 32, and Table 33 show the results of measuring the control time of red rot fungi when the content was changed to 0.2% and 0.3%. It is clear that the disinfection time of each type of acid is significantly shortened as compared with the case where formic acid is not present.

[0046]

[Table 30]

[Table 31]

[Table 32]

[Table 33]

Test Example 11 Extermination Test of Adherent Bacteria The effect of exterminating adherent bacteria was investigated by comparing the viable cell count of Nori leaf bodies treated with the treatment solutions having the compositions shown in Table 35 below with the untreated control group. . The viable cell count was calculated according to the flowchart in Table 34. The results are shown in Table 36.

[Table 34]

[0048]

[Table 35]

[Table 36]

[0049]

[Effects of the Invention] When used alone, the degree of damage to seaweed is high,
Phytic acid, lactic acid,
When used in combination with other acids such as acetic acid and phosphoric acid, the seaweed was not damaged and the bactericidal power of the acid itself was greatly increased, and the red rot fungus could be exterminated in an extremely short time. Since the amount of acid used can be significantly reduced, environmental pollution to the ocean can be greatly reduced.

Claims (3)

[Claims] 1. A solution of formic acid in an aqueous solution of phytic acid, lactic acid, acetic acid, adipic acid, succinic acid, malic acid, citric acid, maleic acid, fumaric acid, gluconic acid, tartaric acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itacone. A disease control agent for cultured seaweed, comprising at least one selected from the group consisting of acid, glutaric acid, ketoglutaric acid, phosphoric acid, hydrochloric acid, sulfuric acid and nitric acid. 2. A formic acid aqueous solution (including part or all of seawater) having a concentration of 0.3 W / V% or less, phytic acid, lactic acid,
Acetic acid, adipic acid, succinic acid, malic acid, citric acid, maleic acid, fumaric acid, gluconic acid, tartaric acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, glutaric acid, ketoglutaric acid, phosphoric acid, hydrochloric acid, sulfuric acid, Whether at least one acid selected from the group consisting of nitric acid is set to a concentration in the aqueous solution of 0.01 to 0.3 W / V% in accordance with the required treatment time, and the cultured seaweed is immersed in this mixed solution. ,
A method for controlling disease of cultured seaweed, which comprises spraying this solution onto cultured seaweed. 3. A phosphoric acid, fumaric acid, ketoglutaric acid, lactic acid, sulfuric acid, nitric acid, acetic acid, glutaric acid having a concentration of 0.01-0.1 W / V% of a formic acid aqueous solution (including a case where it is partially or wholly seawater). Acid, tartaric acid, itaconic acid, adipic acid, succinic acid,
At least one acid selected from the group consisting of citric acid, gluconic acid, maleic acid, malic acid, phytic acid, and hydrochloric acid is used in the concentration range of 0.05 to 0.3 W / V% in the aqueous solution. Then, the method for controlling short-term diseases of cultured seaweed is characterized by immersing the cultured seaweed in this mixed solution or spraying this solution on the cultured seaweed.