JPH08501924A - ポリサッカリド結合性融合タンパク質と抱合体 - Google Patents
ポリサッカリド結合性融合タンパク質と抱合体Info
- Publication number
- JPH08501924A JPH08501924A JP5517877A JP51787793A JPH08501924A JP H08501924 A JPH08501924 A JP H08501924A JP 5517877 A JP5517877 A JP 5517877A JP 51787793 A JP51787793 A JP 51787793A JP H08501924 A JPH08501924 A JP H08501924A
- Authority
- JP
- Japan
- Prior art keywords
- polysaccharide
- binding
- substrate
- cellulose
- polysaccharidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H8/00—Macromolecular compounds derived from lignocellulosic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B15/00—Preparation of other cellulose derivatives or modified cellulose, e.g. complexes
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01074—Glucan 1,4-beta-glucosidase (3.2.1.74)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/005—Microorganisms or enzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
- G01N2400/24—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar beta-D-Glucans, i.e. having beta 1,n (n=3,4,6) linkages between saccharide units, e.g. xanthan
- G01N2400/26—Cellulose
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/813—Carrier is a saccharide
- Y10S530/814—Cellulose or derivatives thereof
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Polymers & Plastics (AREA)
- Materials Engineering (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Neurosurgery (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.融合タンパク質を含むポリサッカリドを得るための方法であって、 ポリサッカリダーゼの基質結合領域と所望のペプチドとを備える融合タンパク 質を調製すること、 上記融合タンパク質と基質を含むポリサッカリドマトリックスとを上記ポリサ ッカリダーゼのための条件下で接触させ、それによって上記基質結合領域を上記 ポリサッカリドマトリックスに結合させること、 を備えていることを特徴とする方法。 2.上記の調製は、 転写の5’-3’方向中に、転写開始調節領域、翻訳開始領域、上記融合タン パク質をコードするDNA配列、及び翻訳と転写終止領域とを含み、上記融合タ ンパク質の発現が上記開始及び終止領域により調節されるDNA構造を包含する 宿主細胞を培養すること、及び上記融合タンパク質を分離すること、を備えてい ることを特徴とする請求の範囲第1項の方法。 3.上記の分離が、上記融合タンパク質と上記ポリサッカリダーゼの基質を備え るマトリッスとを接触させることを含むことを特徴とする請求の範囲第2項の方 法。 4.上記ポリサッカリダーゼがセルラーゼであることを特徴とする請求の範囲第 1項の方法。 5.上記セルラーゼがセルロモナス・フィミ(Cellulomonas fimi)から得られ たものであることを特徴とする請求の範囲第4項の方法。 6.上記セルラーゼがエンドグルカナーゼ(E.C.3.2.1.4)またはセ ロビオヒドロラーセ(E.C.3.2.1.91)であることを特徴とする請求 の範囲第4項の方法。 7.上記ポリサッカリドマトリックスが不溶性セルロースであることを特徴とす る請求の範囲第4項の方法。 8.上記不溶性セルロースが微晶質セルロース、リグノセルロース性材料または 綿であることを特徴とする請求の範囲第7項の方法。 9.上記リグノセルロース性材料が、木材、木材パルプ、または植物組織を含む ことを特徴とする請求の範囲第7項の方法。 10.上記ポリサッカリドマトリックスが可溶性ポリサッカリドであることを特 徴とする請求の範囲第4項の方法。 11.上記可溶性ポリサッカリドがカルボキシメチルセルロースであることを特 徴とする請求の範囲第10項の方法。 12.構成材料として、 (1)(a)化学的一部が接合され、(b)ポリサッカリダーゼの、基質結合 領域を含むアミノ酸配列、またはそれの機能的部分、を備える抱合体、及び (2)上記ポリサッカリダーゼのポリサッカリド基質を含むポリサッカリドマ トリックス、 とを備え、上記構成材料(1)は、上記(b)を介して構成材料(2)に結合さ れていることを特徴とする組成物。 13.上記ポリサッカリダーゼが、β−1,4−グリカナーゼであり、かつ上記 ポリサッカリド基質がβ−1,4−グリカンを含むことを特徴とする請求の範囲 第12項の組成物。 14.上記β−1,4−グリカンはセルロースまたはキチンを含むことを特徴と する請求の範囲第13項の組成物。 15.上記セルロースが、紙、微晶質セルロース、綿及びカルボキシメチルセル ロースよりなる群から選択されたものであることを特徴とする請求の範囲第14 項の組成物。 16.上記ポリサッカリダーゼの上記基質結合領域に上記ポリサッカリダーゼの ための基質を接触させる条件下で、上記基質結合領域に上記マトリックス中のポ リサッカリド基質を結合させる方法によって得られたことを特徴とする請求の範 囲第12項の組成物。 17.上記化学的一部がポリペプチドに融合されることを特徴とする請求の範囲 第12項の組成物。 18.上記化学的一部が、 (a)放射性核種、 (b)発色団、染料、色素、酵素及びフルオレセイン よりなる群から選択されることを特徴とする請求の範囲第17項の組成物。 19.上記染料がクマシーブルーを含むことを特徴とする請求の範囲第18項の 組成物。 20.上記発色団が、 (a)フルオレセインイソチオシアナート、及び (b)テトラメチルローダミンイソチオシアナート、 よりなる群から選択されたものであることを特徴とする請求の範囲第18項の組 成物。 21.上記ポリサッカリドマトリックスが不溶性セルロースであることを特徴と する請求の範囲第12項の組成物。 22.上記不溶性セルロースが微晶質セルロース、紙、綿、木材、パルプまたは 植物組織であることを特徴とする請求の範囲第21項の組成物。 23.上記ポリサッカリドマトリックスが可溶性ポリサッカリドであることを特 徴とする請求の範囲第12項の組成物。 24.上記可溶性ポリサッカリドがカルボキシメチルセルロースであることを特 徴とする請求の範囲第23項の組成物。 25.(a)化学的一部が接合され、(b)ポリサッカリダーゼから調製し得る 、基質結合領域を含むアミノ酸配列、またはそれの機能的部分、を備える除去可 能標識。 26.請求の範囲第25項の除去可能標識を備え、そこでポリサッカリドが上記 ポリサッカリダーゼのための基質であり、かつ上記除去可能標識が上記ポリサッ カリドから上記アミノ酸配列を通して結合されたことを特徴とするポリサッカリ ド。 27.上記アミノ酸配列がプロテアーゼ認識配列を含むことを特徴とする請求の 範囲第26項のポリサッカリド。 28.上記プロテアーゼ認識配列がポリサッカリダーゼに固有のものであること を特徴とする請求の範囲第27項のポリサッカリド。 29.上記プロテアーゼ認識配列がポリサッカリダーゼと異種であることを特徴 とする請求の範囲第27項のポリサッカリド。 30.上記プロテアーゼ認識配列が、 ポリサッカリドと(a)低イオン強度及び(b)高pHよりなる群から選択さ れる特徴をもった除去溶液と接触させること、を含む方法によって除去され得る ものであることを特徴とする請求の範囲第26項のポリサッカリド。 31.上記プロテアーゼ認識配列が、 ポリサッカリドと、上記プロテアーゼ認識配列で上記アミノ酸配列を分割し得 るプロテアーゼを備える除去溶液とを接触させること、を含む方法によって除去 され得るものであることを特徴とする請求の範囲第26項のポリサッカリド。 32.上記プロテアーゼが非特異性プロテアーゼであることを特徴とする請求の 範囲第31項のポリサッカリド。 33.上記除去可能標識が、上記化学的一部と上記アミノ酸配列との間の特異的 プロテアーゼ分割部位を更に含んだことを特徴とする請求の範囲第26項のポリ サッカリド。 34.上記特異的プロテアーゼ分割部位が、 (a)因子Xa、 (b)トロンビン、及び (c)トリプシン、 よりなる群から選択されるプロテアーゼのためのものであることを特徴とする請 求の範囲第33項のポリサッカリド。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US07/865,095 US5340731A (en) | 1988-07-08 | 1992-04-08 | Method of preparing a B-1,4 glycan matrix containing a bound fusion protein |
US07/865,095 | 1992-04-08 | ||
PCT/CA1993/000149 WO1993021331A2 (en) | 1992-04-08 | 1993-04-06 | Polysaccharide binding fusion proteins and conjugates |
Publications (1)
Publication Number | Publication Date |
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JPH08501924A true JPH08501924A (ja) | 1996-03-05 |
Family
ID=25344702
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP5517877A Pending JPH08501924A (ja) | 1992-04-08 | 1993-04-06 | ポリサッカリド結合性融合タンパク質と抱合体 |
Country Status (5)
Country | Link |
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US (4) | US5340731A (ja) |
EP (1) | EP0635059A1 (ja) |
JP (1) | JPH08501924A (ja) |
CA (1) | CA2117887A1 (ja) |
WO (1) | WO1993021331A2 (ja) |
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- 1993-04-06 JP JP5517877A patent/JPH08501924A/ja active Pending
- 1993-04-06 EP EP93907724A patent/EP0635059A1/en not_active Ceased
- 1993-04-06 CA CA002117887A patent/CA2117887A1/en not_active Abandoned
-
1994
- 1994-05-24 US US08/249,037 patent/US5928917A/en not_active Expired - Fee Related
-
1997
- 1997-01-23 US US08/788,621 patent/US6124117A/en not_active Expired - Fee Related
- 1997-01-24 US US08/788,622 patent/US5962289A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US6124117A (en) | 2000-09-26 |
US5928917A (en) | 1999-07-27 |
WO1993021331A2 (en) | 1993-10-28 |
CA2117887A1 (en) | 1993-10-09 |
US5340731A (en) | 1994-08-23 |
WO1993021331A3 (en) | 1994-01-06 |
EP0635059A1 (en) | 1995-01-25 |
US5962289A (en) | 1999-10-05 |
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