JPH08318279A - Sterilizing water for spore bacteria and sterilization of spore bacteria - Google Patents
Sterilizing water for spore bacteria and sterilization of spore bacteriaInfo
- Publication number
- JPH08318279A JPH08318279A JP8100166A JP10016696A JPH08318279A JP H08318279 A JPH08318279 A JP H08318279A JP 8100166 A JP8100166 A JP 8100166A JP 10016696 A JP10016696 A JP 10016696A JP H08318279 A JPH08318279 A JP H08318279A
- Authority
- JP
- Japan
- Prior art keywords
- water
- residual chlorine
- sterilizing
- sterilized
- spore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、食器、食品に付着
する芽胞菌の殺菌を始めとして、調理環境衛生用、手洗
い用、食品材料用及びおしぼり用等、広範囲の分野に用
いられる芽胞菌用の殺菌水及び該殺菌水を用いた芽胞菌
の殺菌方法に関し、更に詳しくは、塩化ナトリウム水溶
液（以下、「食塩水」という）の有隔膜電解によって得
られる芽胞菌用の殺菌水及び該殺菌水を用いた芽胞菌の
殺菌方法に関する。TECHNICAL FIELD The present invention relates to spore germs used in a wide range of fields such as sterilization of spore germs adhering to tableware and foods, hygiene for cooking environment, hand washing, food materials and hand towels. Sterilized water and a sterilizing method for spore bacterium using the sterilized water, more specifically, sterilized water for spore bacterium and the sterilized water obtained by diaphragm electrolysis of an aqueous sodium chloride solution (hereinafter referred to as "saline solution"). The present invention relates to a method for sterilizing a spore bacterium using a.
【０００２】[0002]
【従来の技術】従来、細菌用の殺菌水には種々のものが
知られており、例えば、食器や食品等の殺菌水として
は、次亜塩素酸や次亜塩素酸ナトリウムの水溶液が知ら
れており、その使用方法は大黒勇・他著「新版・細菌
学」（建帛社 昭和５５年初版発行）などに記載されて
いる。2. Description of the Related Art Conventionally, various kinds of sterilizing water for bacteria have been known. For example, as sterilizing water for tableware, foods, etc., an aqueous solution of hypochlorous acid or sodium hypochlorite is known. The method of use is described in Isamu Oguro, et al., "New Edition, Bacteriology" (Kensha Co., Ltd., first edition published in 1980).
【０００３】[0003]
【発明が解決しようとする課題】ところが、前記した次
亜塩素酸や次亜塩素酸ナトリウムは、その水溶液で、更
に強力な殺菌効果を発揮せしめて芽胞菌などを殺菌する
ためには、使用現場でＨＣｌなどを添加しｐＨをコント
ロールすることが必要とされる。このため、一般家庭や
料理店の厨房等で食品類などの殺菌に用いるには取扱い
上の安全性及び食品衛生法上などの面で不都合があり、
また食品工場等の大規模施設で用いるには、食品衛生法
上の問題に加えて、多量に必要となることから殺菌に要
するコストも高くなるという問題がある。本発明は、上
記した問題点を解決して、調理環境衛生用、手洗い用、
食品材料用及びおしぼり用等、広範囲の分野における消
毒に使用しうる安全で、かつ、低コストで製造できる殺
菌水を提供することを目的とする。However, the above-mentioned aqueous solution of hypochlorous acid or sodium hypochlorite has a strong bactericidal effect in order to sterilize spores and the like at the site of use. It is necessary to control the pH by adding HCl or the like. Therefore, it is inconvenient in terms of safety in handling and food hygiene law when used for sterilizing foods in kitchens of general households and restaurants,
In addition, when used in a large-scale facility such as a food factory, in addition to the problem of the Food Sanitation Law, there is a problem that a large amount is required and thus the cost required for sterilization becomes high. The present invention solves the above problems, for cooking environment hygiene, for hand washing,
It is an object of the present invention to provide safe and low-cost sterilizing water that can be used for disinfection in a wide range of fields such as food materials and hand towels.
【０００４】[0004]
【課題を解決するための手段】本発明は、上記した目的
を達成せんとしてなされたもので、塩化ナトリウム水溶
液の有隔膜電解によって得られる殺菌水であって、該殺
菌水がアノード室から排出される電解水であること及び
残留塩素を少なくとも４０ppm 含有すると共にｐＨが
６．５以下であることを特徴とする殺菌水を提供するも
のである。The present invention has been made to achieve the above-mentioned object, and is sterilized water obtained by diaphragm electrolysis of an aqueous sodium chloride solution, the sterilized water being discharged from the anode chamber. Disclosed is sterilized water which is electrolyzed water containing at least 40 ppm of residual chlorine and having a pH of 6.5 or less.
【０００５】残留塩素とは、殺菌水中に残留する遊離塩
素のことを言い、例えば、Ｃｌ2 （ガス状で水中に存在
する塩素及び水中に溶解した塩素）、ＨＣｌＯ（次亜塩
素酸）、Ｃｌ2 Ｏ（一酸化二塩素）、ＣｌＯ- （次亜塩
素酸イオン）を指す。残留塩素濃度の測定は、例えば、
オルトトリジン法、ヨウ化カリウム試薬による検定法に
よって行なうことができる。Residual chlorine means free chlorine remaining in sterilized water, for example, Cl 2 (chlorine existing in water in a gaseous state and chlorine dissolved in water), HClO (hypochlorous acid), Cl 2 O (dichlorine monoxide), ClO − (hypochlorite ion). The residual chlorine concentration can be measured, for example, by
It can be carried out by the orthotolidine method or an assay method using a potassium iodide reagent.
【０００６】本発明の殺菌水は、一定濃度の食塩水をア
ノードとカソードを配した有隔膜電解槽中で電解に付す
ることにより容易に製造することができる。電解に用い
る装置としては、既存の装置を用いることもできるが、
本発明の殺菌水の製造に特に改良された装置（後述）を
用いることもできる。製造は、回分式のものでも連続式
のものでもよいが、工業的には連続式のものが好まし
い。The sterilized water of the present invention can be easily manufactured by subjecting a saline solution having a constant concentration to electrolysis in a diaphragm electrolytic cell having an anode and a cathode. As an apparatus used for electrolysis, an existing apparatus can be used,
It is also possible to use a particularly improved apparatus (described later) for producing the sterilizing water of the present invention. The production may be either a batch type or a continuous type, but industrially a continuous type is preferable.
【０００７】電極は、アノードについては、有害な金属
イオンが溶出することなく、耐食性があり、遊離塩素が
効率よく発生するよう塩素過電圧が小さく酸素過電圧が
大きい電極である必要があり、例えばチタン基板に白金
及びイリジウムをコーティングした電極などが好まし
い。カソードについては、耐食性に優れていることが条
件である。Regarding the electrode, as for the anode, it is necessary that the electrode be corrosion resistant without eluting harmful metal ions, and have a small chlorine overvoltage and a large oxygen overvoltage so that free chlorine can be efficiently generated. For example, a titanium substrate. Electrodes coated with platinum and iridium are preferable. The cathode is required to have excellent corrosion resistance.
【０００８】用いる食塩水の濃度は、好ましくは０．０
３〜３６％、より好ましくは０．０５〜３％である。
０．０３％より低い濃度では、電解効率が悪く消費電力
の損失が大きく、また３６％、すなわち２０℃における
飽和濃度（３６ｇ／１００ｇ水）より濃度が高いと塩化
ナトリウムが析出して沈殿し、殺菌水を高精度、かつ、
安定して得ることができない。The concentration of the saline solution used is preferably 0.0
It is 3 to 36%, more preferably 0.05 to 3%.
When the concentration is lower than 0.03%, the electrolysis efficiency is poor and the loss of power consumption is large, and when the concentration is higher than 36%, that is, the saturated concentration at 20 ° C. (36 g / 100 g water), sodium chloride precipitates and precipitates, Highly accurate sterilizing water
You can't get stable.
【０００９】電流密度は、通常５０〜２００A/m2、好ま
しくは１００〜１５０A/m2である。[0009] The current density is usually 50-200 / m 2, preferably 100~150A / m 2.
【００１０】また、食塩水の電解に際しては、アノード
側に被電解水、すなわち食塩水を収容するが、電解に伴
うアノード室の昇温を抑制すべく、例えば、冷却水等を
用いて液温が４０℃以下に維持されるように制御する。
食塩水の液温が４０℃以上になると、消費電力が上昇し
始め電解効率が低下し、且つ電極が劣化し易くなる。In electrolysis of saline solution, electrolyzed water, that is, saline solution, is stored on the anode side. However, in order to suppress the temperature rise of the anode chamber due to electrolysis, for example, cooling water is used to control the liquid temperature. Is controlled to be 40 ° C. or lower.
When the liquid temperature of the salt solution is 40 ° C. or higher, the power consumption starts to increase and the electrolysis efficiency decreases, and the electrodes are likely to deteriorate.
【００１１】かかる殺菌水は、アノード室から排出され
る酸性の電解水を原水として中性水及び／又はカソード
室から排出されるアルカリ水と混合し、殺菌の目的に応
じた残留塩素濃度やｐＨ値に調節して使用することも可
能であり、かかる調節によっても残留塩素濃度が１．０
〜２００ppm の範囲にあれば殺菌効果が保持される。こ
こで、残留塩素濃度やｐＨの調節のために用いるアルカ
リ水として、上記食塩水電解の際にカソード側において
得られるＮａＯＨ含有水を用いているが、勿論、系外か
らＮａＯＨ水溶液を添加しても良い。The sterilized water is prepared by mixing the acidic electrolyzed water discharged from the anode chamber with the neutral water and / or the alkaline water discharged from the cathode chamber as raw water to obtain a residual chlorine concentration or pH depending on the purpose of sterilization. It is also possible to adjust the value to use, and the residual chlorine concentration is 1.0
If it is in the range of up to 200 ppm, the bactericidal effect is maintained. Here, as the alkaline water used for adjusting the residual chlorine concentration and the pH, the NaOH-containing water obtained on the cathode side during the electrolysis of the saline solution is used. Of course, an NaOH aqueous solution may be added from outside the system. Is also good.
【００１２】また、本発明の殺菌水は通常ｐＨ２．０〜
７．２、好ましくは２．５〜６．５、最も好ましくは
３．０〜６．０を有する。ｐＨが７．２より大きいと、
ＣｌＯ- が増大して殺菌効果が低下し、不安定となる
し、一方２．０より小さいとＨＣｌＯの存在が不安定と
なるからである。なお、芽胞菌を殺菌対象とする場合に
は、残留塩素濃度４０ppm 以上を確保すると共にｐＨ
は、６．５以下になるように調節した方がよい。The sterilizing water of the present invention usually has a pH of 2.0-2.0.
7.2, preferably 2.5 to 6.5, most preferably 3.0 to 6.0. If the pH is greater than 7.2,
This is because ClO − increases and the bactericidal effect decreases and becomes unstable, while when it is less than 2.0, the presence of HClO becomes unstable. When spores are to be sterilized, ensure a residual chlorine concentration of 40 ppm or higher and
Should be adjusted to 6.5 or less.
【００１３】さらに、残留塩素Ｃｌ2 、ＨＣｌＯ及びＣ
ｌＯ- の中で最も酸化力が強いのはＨＣｌＯであるが、
ｐＨ５．５以上でＨＣｌＯは電離してＣｌＯ- になり始
め、ｐＨが大きくなるに従いＣｌＯ- の存在比が増加す
ることから、殺菌力をより強く保持するためにはＣｌＯ
- が２５％以下であることが好ましい（第２図、残留遊
離塩素の存在比参照）。Furthermore, residual chlorine Cl 2 , HClO and C
lO - Although most oxidizing power is strong in is the HClO,
When the pH is 5.5 or more, HClO is ionized to become ClO − , and the abundance ratio of ClO − increases as the pH increases.
- it is preferably at most 25% (Figure 2, reference abundance of residual free chlorine).
【００１４】また、本発明の殺菌水の使用方法として
は、食器、食品等の被消毒対象上に直接散布したり、食
品の調理水として直接使用する等種々の使用方法があ
り、食塩水の電解によって得られる殺菌水であることか
ら、食品衛生法上等の面からも特に殺菌水としての使用
方法においては何ら問題はない。The sterilizing water of the present invention can be used by spraying it directly onto the object to be disinfected such as tableware and food, or by directly using it as cooking water for food. Since it is sterilized water obtained by electrolysis, there is no problem in terms of food hygiene and the like particularly in the method of use as sterilized water.
【００１５】本発明の殺菌水は、食器、食品等の殺菌水
としてはもとより、水道水の殺菌水にも用いることが可
能である。とりわけ、これまでオートクレーブ等による
加圧・高温滅菌をしないかぎり殺菌が困難であるとされ
てきた芽胞菌にも優れた殺菌効果を有する。The sterilized water of the present invention can be used not only as sterilized water for tableware, foods, etc., but also as tap water sterilized water. In particular, it has an excellent bactericidal effect even on spore bacterium, which has been difficult to sterilize unless it is sterilized under pressure and high temperature by autoclave.
【００１６】[0016]
【実施例】以下、本発明の一実施例を図面を参照しつつ
説明する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings.
【００１７】先ず、図示の製造装置１を用いて本発明の
殺菌水を製造した。すなわち、濃度５％の食塩水５ L
（リットル。以下、同様）を貯留した貯留槽２から、定
量ポンプ３により流量０．５〜３．０ L/minの中性水を
供給する中性水供給管４に１０〜５０ ml/min の食塩水
を供給して中性水と混合させ、容量０．６ Lの方形の電
解槽５の両側壁５ａ，５ａ、多孔質隔膜６，６及びチタ
ンに白金とイリジウムをコーティングしたアノード７で
囲まれたアノード室８，８へ供給した。First, the sterilizing water of the present invention was manufactured using the manufacturing apparatus 1 shown. That is, 5 L of salt solution with a concentration of 5%
10 to 50 ml / min to the neutral water supply pipe 4 for supplying the neutral water with a flow rate of 0.5 to 3.0 L / min from the storage tank 2 which stores (liter. Is supplied to mix with neutral water, and the side walls 5a and 5a of the rectangular electrolytic cell 5 having a capacity of 0.6 L, the porous diaphragms 6 and 6 and the anode 7 in which titanium is coated with platinum and iridium are used. It was supplied to the enclosed anode chambers 8, 8.
【００１８】一方、前記両隔膜６，６の外側に設けたＳ
ＵＳ３０４からなるカソード９，９と両側壁５ａ，５ａ
により形成されるカソード室１０，１０に、前記両アノ
ード室８，８を冷却するため導水管１１，１２を通って
中性水を０．５〜３．０ L/min供給し、アノード室８，
８を冷却するとともに、電解によりアルカリ性となった
前記中性水（以下、アルカリ水という）をカソード室１
０，１０の上部に設けた導水管１３，１３により０．５
〜３．０ L/min排出した。ここで、前記食塩水の電解に
際して両極７，９間に印加した電流は１２Ａであった。On the other hand, S provided outside the both diaphragms 6 and 6.
US304 cathode 9,9 and both side walls 5a, 5a
0.5 to 3.0 L / min of neutral water is supplied to the cathode chambers 10, 10 formed by the above through the water conduits 11, 12 for cooling the anode chambers 8, 8. ，
The neutral water (hereinafter, referred to as alkaline water) that has become alkaline by electrolysis while cooling 8
0.5 by the water pipes 13 and 13 installed on the upper part of 0 and 10.
~ 3.0 L / min was discharged. Here, the current applied between both electrodes 7 and 9 during electrolysis of the saline solution was 12A.
【００１９】そして、かかる電解によって前記アノード
室８，８に生成された酸性の水（以下、電解水という）
を０．５〜３．０L/min で導水管１４により密閉式の希
釈撹拌槽１５へ導き、前記導水管１１に連通する導水管
１６により０．５〜３．０ L/minの流量で供給される中
性水及び／又は前記導水管１３により０．５〜３．０L/
minの流量で供給されるアルカリ水によって希釈撹拌
し、排出管１７より殺菌水を１．０〜６．０ L/min得
た。Acidic water (hereinafter referred to as electrolyzed water) produced in the anode chambers 8 and 8 by such electrolysis.
At a flow rate of 0.5 to 3.0 L / min by a water guide pipe 16 communicating with the water guide pipe 11 at a flow rate of 0.5 to 3.0 L / min. 0.5 to 3.0 L / depending on the neutral water and / or the water conduit 13
The mixture was diluted and stirred with alkaline water supplied at a flow rate of min, and 1.0 to 6.0 L / min of sterile water was obtained from the discharge pipe 17.
【００２０】尚、バルブ１８はアノード室８，８へ供給
する食塩水の濃度調整用、バルブ１９は電解水又は殺菌
水を希釈する中性水の調整用、バルブ２０は電解水又は
殺菌水を希釈するアルカリ水の調整用、バルブ２１はア
ルカリ水の排出用である。The valve 18 is for adjusting the concentration of the saline solution supplied to the anode chambers 8, 8, the valve 19 is for adjusting the neutral water for diluting the electrolyzed water or the sterilized water, and the valve 20 is for the electrolyzed water or the sterilized water. The valve 21 is for adjusting the alkaline water to be diluted, and the valve 21 is for discharging the alkaline water.
【００２１】実施例１〜４ 前記装置１を用いて、電解電流１２Ａで濃度０．０７％
の食塩水を電解し、得られた電解直後の電解水をカソー
ド室１０から導水管１３により排出されたアルカリ水を
用いて４種類の混合比で混合し、４種類の殺菌水を得
た。但し、実施例１、２の電解水はアルカリ水の流量を
０．５ L/minで、実施例３、４は１ L/minで製造した。Examples 1 to 4 Using the apparatus 1 described above, a concentration of 0.07% was obtained at an electrolytic current of 12A.
Electrolyzed saline solution was obtained, and the obtained electrolyzed water immediately after electrolysis was mixed with alkaline water discharged from the cathode chamber 10 through the water conduit 13 at four mixing ratios to obtain four kinds of sterilized water. However, the electrolyzed water of Examples 1 and 2 was produced at a flow rate of alkaline water of 0.5 L / min, and Examples 3 and 4 were produced at 1 L / min.
【００２２】上記４種類の殺菌水１０ ml と１ ml 中に
１０6 個の芽胞菌すなわち黒コショウバチルスを含む菌
液０．１ ml とを２５℃のもとで混合することにより上
記菌に殺菌水を約１０分間接触させた後、混合液中の残
菌数を平板混釈培養法により測定した。その結果をアル
カリ水との混合比、殺菌水の残留塩素濃度及びｐＨとと
もに表１に示した。なお、残留塩素濃度はオルトトリジ
ン法により、ｐＨは既存のｐＨメーターによりそれぞれ
測定した。10 ml of the above-mentioned four kinds of sterilized water and 0.1 ml of a spore solution containing 10 6 spore bacteria, that is, black pepper bacillus in 1 ml are mixed at 25 ° C. to sterilize the bacteria. After contact with water for about 10 minutes, the number of residual bacteria in the mixed solution was measured by the plate pour culture method. The results are shown in Table 1 together with the mixing ratio with alkaline water, the residual chlorine concentration and pH of sterilized water. The residual chlorine concentration was measured by the orthotolidine method, and the pH was measured by an existing pH meter.
【００２３】[0023]
【表１】 [Table 1]
【００２４】実施例５〜１２ 前記した製造装置１を用いて８種類の殺菌水を製造し、
これらの殺菌水にカソード側から採取した水酸化ナトリ
ウム含有のアルカリ水を添加してｐＨを調整し、さらに
残留塩素濃度が１５ ppmおよび４０ ppmとなるように調
整した。これを用いて黒コショウバチルスに対する殺菌
効果を前記実施例と同様の方法で試験した。その結果を
表２に示した。Examples 5 to 12 Eight kinds of sterilized water were produced using the production apparatus 1 described above,
To these sterilized waters, sodium hydroxide-containing alkaline water sampled from the cathode side was added to adjust the pH, and the residual chlorine concentrations were adjusted to 15 ppm and 40 ppm. Using this, the bactericidal effect against black pepper Bacillus was tested in the same manner as in the above-mentioned Examples. The results are shown in Table 2.
【００２５】[0025]
【表２】 [Table 2]
【００２６】実施例１３ 前記した製造装置１を用いて、濃度１２５０ ppmの食塩
水を、電圧７Ｖ、電解電流６Ａで電解した。アノード室
から排出される電解水はｐＨ２．７、残留塩素濃度７０
ppm、カソード室から排出されるアルカリ水はｐＨ１
１．４、残留塩素濃度０．３ ppmであった。アノード室
から排出される電解水１ Lに、導水管１１，１６を経由
して希釈撹拌槽１５へ送られる中性水４ Lを混合させ
た。その結果、ｐＨ６．１、残留塩素濃度１５ ppmの殺
菌水５ Lが得られた。Example 13 Using the manufacturing apparatus 1 described above, a saline solution having a concentration of 1250 ppm was electrolyzed at a voltage of 7 V and an electrolytic current of 6 A. The electrolyzed water discharged from the anode chamber has a pH of 2.7 and a residual chlorine concentration of 70.
ppm, pH of alkaline water discharged from the cathode chamber is 1
The residual chlorine concentration was 1.4 and the concentration was 0.3 ppm. 1 L of electrolyzed water discharged from the anode chamber was mixed with 4 L of neutral water sent to the dilution stirring tank 15 via the water conduits 11 and 16. As a result, 5 L of sterilized water having a pH of 6.1 and a residual chlorine concentration of 15 ppm was obtained.
【００２７】実施例１４ 実施例１３で得られた電解水１ Lに、中性水４L とカソ
ード室１０から導水管１３により排出されたアルカリ水
０．３ Lを混合させた。その結果、ｐＨ７．０、残留塩
素濃度１５ ppmの殺菌水５．３ Lが得られた。Example 14 1 L of electrolyzed water obtained in Example 13 was mixed with 4 L of neutral water and 0.3 L of alkaline water discharged from the cathode chamber 10 through the water conduit 13. As a result, 5.3 L of sterilized water having a pH of 7.0 and a residual chlorine concentration of 15 ppm was obtained.
【００２８】実施例１３及び１４で得られた殺菌水５ml
と１ml中に１０8 個の大腸菌を含む菌液５mlを２５℃の
もとで混合することにより、上記菌に殺菌水を約１分間
接触させた後、混合液中の残菌数を平板浸釈培養法によ
り測定した。その結果を表３に示した。5 ml of sterilized water obtained in Examples 13 and 14
And 5 ml of a bacterial solution containing 10 8 Escherichia coli in 1 ml were mixed at 25 ° C to bring the above bacteria into contact with sterilized water for about 1 minute, and the number of remaining bacteria in the mixed solution was plated. It was measured by the culture method. Table 3 shows the results.
【００２９】[0029]
【表３】 [Table 3]
【００３０】実施例１５ 前記した製造装置１を用いて、濃度１２５０ ppmの食塩
水を、電圧１２Ｖ、電解電流１１Ａで電解した。アノー
ド室から排出される電解水はｐＨ２．８、残留塩素濃度
１６０ ppm、カソード室から排出されるアルカリ水はｐ
Ｈ１１．５、残留塩素濃度０．３ ppmであった。このア
ノード室から排出される電解水１ Lに、導水管１１，１
６を経由して希釈撹拌槽１５へ送られる中性水３ Lを混
合させた。その結果、ｐＨ５．８、残留塩素濃度４０ p
pmの殺菌水４ Lが得られた。Example 15 Using the manufacturing apparatus 1 described above, a saline solution having a concentration of 1250 ppm was electrolyzed at a voltage of 12 V and an electrolytic current of 11 A. The electrolyzed water discharged from the anode chamber has a pH of 2.8, the residual chlorine concentration is 160 ppm, and the alkaline water discharged from the cathode chamber is p.
H11.5 and residual chlorine concentration were 0.3 ppm. 1 L of electrolyzed water discharged from this anode chamber is connected to the water conduits 11, 1
3 L of neutral water sent to the dilution stirring tank 15 via 6 was mixed. As a result, pH 5.8, residual chlorine concentration 40 p
4 L of sterile water of pm was obtained.
【００３１】実施例１６ 実施例１５で得られたアノード室から排出される電解水
１ Lに、中性水３ Lとカソード室１０から導水管１３に
より排出されたアルカリ水０．３ Lを混合させた。その
結果、ｐＨ６．６、残留塩素濃度４０ppm の殺菌水４．
３ Lが得られた。Example 16 1 L of electrolyzed water discharged from the anode chamber obtained in Example 15 was mixed with 3 L of neutral water and 0.3 L of alkaline water discharged from the cathode chamber 10 through the conduit 13. Let As a result, sterilized water with pH 6.6 and residual chlorine concentration of 40 ppm 4.
3 L was obtained.
【００３２】実施例１５及び１６で得られた殺菌水１０
ml と１ ml 中に１０6 個の芽胞菌すなわち黒コショウ
バチルスを含む菌液０．５ ml を２５℃のもとで混合す
ることにより、上記菌に殺菌水を約１０分間接触させた
後、混合液中の残菌数を平板浸釈培養法により測定し
た。その結果を表４に示した。Sterilized water 10 obtained in Examples 15 and 16
After contacting the above bacteria with sterilized water for about 10 minutes, by mixing 0.5 ml of the bacterial solution containing 10 6 spore bacterium, that is, black pepper bacillus in 1 ml with 25 ml at 25 ° C, The number of residual bacteria in the mixed solution was measured by the plate dipping culture method. The results are shown in Table 4.
【００３３】[0033]
【表４】 [Table 4]
【００３４】[0034]
【発明の効果】以上詳述した如く、本発明の殺菌水は安
全で取扱の容易な食塩水の電解によって安定的に製造で
きるばかりか、残留塩素濃度とｐＨを任意の値に設定で
きるので、食器、食品等を始めとして、調理環境衛生
用、手洗い用、食品材料用、おしぼり用の殺菌に、さら
には、食品加工流通分野、飲用水、プール用水、医療分
野等、広範囲の分野における殺菌に使用できることは当
然のこととして、これまでオートクレーブ等による加圧
・高温滅菌しない限り殺菌が困難であるとされてきた芽
胞菌にも優れた殺菌効果を有し、且つ低コストで製造で
きる等の顕著な効果がある。As described in detail above, the sterilized water of the present invention can be stably produced by electrolysis of saline which is safe and easy to handle, and the residual chlorine concentration and pH can be set to arbitrary values. For tableware, food, etc., for sterilization for cooking environment hygiene, hand washing, food materials, hand towel, and for a wide range of fields such as food processing distribution field, drinking water, pool water, medical field, etc. As a matter of course, it can be used, and it has an excellent bactericidal effect even on spore bacteria that have been difficult to sterilize until now unless it is sterilized under pressure and high temperature by an autoclave, etc. It has a great effect.
【図１】本発明の殺菌水を製造する装置の一例を示す概
略図である。FIG. 1 is a schematic view showing an example of an apparatus for producing sterilizing water of the present invention.
【図２】残留遊離塩素の存在比を示す説明図である。FIG. 2 is an explanatory diagram showing an abundance ratio of residual free chlorine.
１…製造装置 ２…貯留槽 ４…中性水供給管 ５…電解槽 ７…アノード ８…アノード室 ９…カソード 10…カソード室 11、12、 16 …導水管 13…アルカリ水の導水管 14…電解水の導水管 15…希釈撹拌槽 17…殺菌水の排出管 DESCRIPTION OF SYMBOLS 1 ... Manufacturing apparatus 2 ... Storage tank 4 ... Neutral water supply pipe 5 ... Electrolyte tank 7 ... Anode 8 ... Anode chamber 9 ... Cathode 10 ... Cathode chamber 11, 12, 16 ... Water conduit 13 ... Alkaline water conduit 14 ... Electrolytic water conduit 15 ... Dilution stirring tank 17 ... Sterilized water discharge pipe
─────────────────────────────────────────────────────
─────────────────────────────────────────────────── ───
【手続補正書】[Procedure amendment]
【提出日】平成８年５月２２日[Submission date] May 22, 1996
【手続補正１】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】特許請求の範囲[Name of item to be amended] Claims
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【特許請求の範囲】[Claims]
【手続補正２】[Procedure Amendment 2]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】０００３[Name of item to be corrected] 0003
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【０００３】[0003]
【発明が解決しようとする課題】ところが、前記した次
亜塩素酸や次亜塩素酸ナトリウムは、その水溶液で、更
に強力な殺菌効果を発揮せしめて芽胞菌を殺菌するため
には、使用現場でＨＣｌなどを添加しｐＨをコントロー
ルすることが必要とされる。このため、一般家庭や料理
店の厨房等で食品類などの殺菌に用いるには取扱い上の
安全性及び食品衛生法上などの面で不都合があり、また
食品工場等の大規模施設で用いるには、食品衛生法上の
問題に加えて、多量に必要となることから殺菌に要する
コストも高くなるという問題がある。本発明は、上記し
た問題点を解決して、調理環境衛生用、手洗い用、食品
材料用及びおしぼり用等、広範囲の分野における消毒に
使用しうる安全で、かつ、低コストで製造できる芽胞菌
用の殺菌水及び芽胞菌の殺菌方法を提供することを目的
とする。However, the above-mentioned hypochlorous acid or sodium hypochlorite is an aqueous solution thereof, in order to exert a stronger bactericidal effect and to sterilize the spore bacterium, It is necessary to control the pH by adding HCl or the like. Therefore, it is inconvenient in terms of safety in handling and food hygiene law when used for sterilizing foods in general households and kitchens of restaurants, and also for use in large-scale facilities such as food factories. In addition to the problem of food hygiene law, there is a problem that the cost required for sterilization increases because a large amount is required. The present invention solves the above-mentioned problems, and is a safe spore bacterium that can be used for disinfection in a wide range of fields such as cooking environment hygiene, hand washing, food material and hand towels, and can be produced at low cost. It is an object of the present invention to provide a sterilizing water for sterilization and a sterilizing method of spore bacterium.
【手続補正３】[Procedure 3]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】０００４[Correction target item name] 0004
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【０００４】[0004]
【課題を解決するための手段】本発明は、上記した目的
を達成せんとしてなされたもので、塩化ナトリウム水溶
液の有隔膜電解によって得られる芽胞菌用の殺菌水であ
って、該殺菌水がアノード室から排出される電解水であ
ること及び残留塩素を少なくとも４０ppm 含有すると共
にｐＨが６．５以下であることを特徴とする。SUMMARY OF THE INVENTION The present invention has been made to achieve the above-mentioned object, and is a sterilizing water for spore bacterium obtained by diaphragm electrolysis of an aqueous sodium chloride solution, the sterilizing water being an anode. It is characterized by being electrolyzed water discharged from the chamber, containing at least 40 ppm of residual chlorine, and having a pH of 6.5 or less.
【手続補正４】[Procedure amendment 4]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１１[Correction target item name] 0011
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１１】かかる殺菌水は、アノード室から排出され
る酸性の電解水を原水として中性水及び／又はカソード
室から排出されるアルカリ水と混合し、芽胞菌の種類に
応じた残留塩素濃度やｐＨ値に調節して使用することも
可能であり、かかる調節によっても残留塩素濃度が残留
塩素濃度４０ppm 以上を確保すると共にｐＨは、６．５
以下の範囲にあれば殺菌効果が保持される。ここで、残
留塩素濃度やｐＨの調節のために用いるアルカリ水とし
て、上記食塩水電解の際にカソード側において得られる
ＮａＯＨ含有水を用いているが、勿論、系外からＮａＯ
Ｈ水溶液を添加しても良い。The sterilized water is obtained by mixing the acidic electrolyzed water discharged from the anode chamber with the neutral water and / or the alkaline water discharged from the cathode chamber as raw water to obtain a residual chlorine concentration or a residual chlorine concentration depending on the type of spore bacterium. It is also possible to adjust the pH value before use, and such adjustment also ensures that the residual chlorine concentration is 40 ppm or more and the pH is 6.5.
If it is in the following range, the bactericidal effect is maintained. Here, as the alkaline water used for adjusting the residual chlorine concentration and the pH, the NaOH-containing water obtained on the cathode side during the electrolysis of the saline solution is used.
You may add H aqueous solution.
【手続補正５】[Procedure Amendment 5]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１２[Correction target item name] 0012
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１２】尚、本発明の殺菌水の下限ｐＨとしては、
次亜塩素酸の平衡を考慮すると、２．５以上が好まし
く、３．０以上が更に好ましい。The lower limit pH of the sterilizing water of the present invention is
Considering the equilibrium of hypochlorous acid, 2.5 or more is preferable, and 3.0 or more is further preferable.
【手続補正６】[Procedure correction 6]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１３[Correction target item name] 0013
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１３】殺菌水の使用状態によっては残留塩素濃度
が低い領域においても塩素臭を感じることがあり、使用
環境によってはそれが嫌がられることがあるからであ
る。This is because depending on the use condition of the sterilizing water, a chlorine odor may be felt even in a region where the residual chlorine concentration is low, which may be disliked depending on the use environment.
【手続補正７】[Procedure Amendment 7]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１５[Name of item to be corrected] 0015
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１５】本発明の殺菌水は、食器、食品等の殺菌水
としてはもとより、水道水の殺菌水にも用いることが可
能である。とりわけ、これまでオートクレーブ等による
加圧・高温滅菌をしないかぎり殺菌が困難であるとされ
てきた芽胞菌に対し優れた殺菌効果を有する。The sterilized water of the present invention can be used not only as sterilized water for tableware, foods, etc., but also as tap water sterilized water. In particular, it has an excellent bactericidal effect against spore bacterium, which has been considered to be difficult to sterilize unless it is sterilized under pressure and high temperature by autoclave.
【手続補正８】[Procedure Amendment 8]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００３４[Correction target item name] 0034
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００３４】[0034]
【発明の効果】以上詳述した如く、本発明の殺菌水は安
全で取扱の容易な食塩水の電解によって安定的に製造で
きるばかりか、残留塩素濃度とｐＨを任意の値に設定で
きるので、食器、食品等を始めとして、調理環境衛生
用、手洗い用、食品材料用、おしぼり用の殺菌に、さら
には、食品加工流通分野、飲用水、プール用水、医療分
野等、広範囲の分野における殺菌に使用できることは当
然のこととして、これまでオートクレーブ等による加圧
・高温滅菌しない限り殺菌が困難であるとされてきた芽
胞菌に対して優れた殺菌効果を有し、且つ低コストで製
造できる等の顕著な効果がある。As described in detail above, the sterilized water of the present invention can be stably produced by electrolysis of saline which is safe and easy to handle, and the residual chlorine concentration and pH can be set to arbitrary values. For tableware, food, etc., for sterilization for cooking environment hygiene, hand washing, food materials, hand towel, and for a wide range of fields such as food processing distribution field, drinking water, pool water, medical field, etc. As a matter of course, it can be used, has an excellent bactericidal effect against spore bacterium that has been difficult to sterilize until it is sterilized under pressure and high temperature by autoclave, and can be manufactured at low cost. It has a remarkable effect.
【手続補正９】[Procedure Amendment 9]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】図２[Name of item to be corrected] Figure 2
【補正方法】削除[Correction method] Delete
【手続補正１０】[Procedure Amendment 10]
【補正対象書類名】図面[Document name to be corrected] Drawing
【補正対象項目名】図２[Name of item to be corrected] Figure 2
【補正方法】削除[Correction method] Delete
フロントページの続き (72)発明者 遠藤 正志 岩手県釜石市鈴子町23−15 新日本製鐵株 式會社釜石製鐵所内 (72)発明者 坂下 雅雄 神奈川県川崎市中原区井田1618番地 新日 本製鐵株式會社第１技術研究所内 (72)発明者 大嶋 勝衛 東京都千代田区大手町２−６−３ 新日本 製鐵株式會社内Front page continued (72) Inventor Masashi Endo 23-15, Suzuko-cho, Kamaishi-shi, Iwate Shin Nippon Steel Co., Ltd.Kamaishi Works (72) Inventor Masao Sakashita 1618 Ida, Nakahara-ku, Kawasaki-shi, Kanagawa (1) Inventor Katsue Oshima 2-6-3 Otemachi, Chiyoda-ku, Tokyo Shin-Nippon Steel Corp. In-house
Claims (2)
って得られる芽胞菌用の殺菌水であって、該殺菌水がア
ノード室から排出される電解水であること及び残留塩素
を少なくとも４０ppm 含有すると共にｐＨが６．５以下
であることを特徴とする芽胞菌用の殺菌水。1. A sterilizing water for spore bacterium obtained by diaphragm electrolysis of an aqueous sodium chloride solution, wherein the sterilizing water is electrolyzed water discharged from an anode chamber, and contains at least 40 ppm of residual chlorine and has a pH value. Is 6.5 or less, sterilized water for spore bacterium.
って得られる芽胞菌用の殺菌水であって、該殺菌水がア
ノード室から排出される電解水を中性水及び／又はカソ
ード室から排出されるアルカリ水にて希釈してなること
及び残留塩素を少なくとも４０ppm 含有すると共にｐＨ
が６．５以下であることを特徴とする芽胞菌用の殺菌
水。2. Sterilizing water for spore bacterium obtained by diaphragm electrolysis of an aqueous sodium chloride solution, wherein the sterilizing water is electrolytic water discharged from an anode chamber and discharged from neutral water and / or a cathode chamber. Diluted with alkaline water and containing at least 40ppm residual chlorine and pH
Is 6.5 or less, sterilized water for spore bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8100166A JPH08318279A (en) | 1988-06-06 | 1996-04-22 | Sterilizing water for spore bacteria and sterilization of spore bacteria |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13750388 | 1988-06-06 | ||
| JP63-137503 | 1988-06-06 | ||
| JP8100166A JPH08318279A (en) | 1988-06-06 | 1996-04-22 | Sterilizing water for spore bacteria and sterilization of spore bacteria |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63300998A Division JPH078768B2 (en) | 1988-06-06 | 1988-11-30 | Sterilized water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08318279A true JPH08318279A (en) | 1996-12-03 |
Family
ID=26441251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8100166A Pending JPH08318279A (en) | 1988-06-06 | 1996-04-22 | Sterilizing water for spore bacteria and sterilization of spore bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08318279A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000185285A (en) * | 1998-10-15 | 2000-07-04 | Daikin Ind Ltd | Electrolytic sterilizing water and making apparatus thereof |
| JP2002113469A (en) * | 2000-10-10 | 2002-04-16 | Katayama Chem Works Co Ltd | Method for preventing pollution in industrial water/ wastewater system |
| JP2002205067A (en) * | 2001-01-12 | 2002-07-23 | Hoshizaki Electric Co Ltd | Washing water having sterilizing ability and its manufacturing method |
| US6623695B2 (en) | 1997-12-04 | 2003-09-23 | Steris Corporation | Chemical modification of electrochemically activated solutions for improved performance |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3669857A (en) * | 1970-07-30 | 1972-06-13 | Ionics | ELECTROLYTIC CHLORINATION AND pH CONTROL OF WATER |
| JPS62102890A (en) * | 1985-10-28 | 1987-05-13 | Toyo Kagaku Kenkyusho:Kk | Device for producing sterile water |
-
1996
- 1996-04-22 JP JP8100166A patent/JPH08318279A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3669857A (en) * | 1970-07-30 | 1972-06-13 | Ionics | ELECTROLYTIC CHLORINATION AND pH CONTROL OF WATER |
| JPS62102890A (en) * | 1985-10-28 | 1987-05-13 | Toyo Kagaku Kenkyusho:Kk | Device for producing sterile water |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6623695B2 (en) | 1997-12-04 | 2003-09-23 | Steris Corporation | Chemical modification of electrochemically activated solutions for improved performance |
| JP2000185285A (en) * | 1998-10-15 | 2000-07-04 | Daikin Ind Ltd | Electrolytic sterilizing water and making apparatus thereof |
| JP2002113469A (en) * | 2000-10-10 | 2002-04-16 | Katayama Chem Works Co Ltd | Method for preventing pollution in industrial water/ wastewater system |
| JP2002205067A (en) * | 2001-01-12 | 2002-07-23 | Hoshizaki Electric Co Ltd | Washing water having sterilizing ability and its manufacturing method |
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