JPH08317762A - Propolis composition - Google Patents

Abstract

PURPOSE: To obtain a propolis composition containing essentially the whole hydrophilic organic solvent extract of propolis and water extract of propolis in stable states and exhibiting excellent dispersibility in water. CONSTITUTION: This propolis composition contains ethanol extract of propolis and water extract of propolis. Addition of a saponin to the composition gives a propolis composition having high water dispersibility. The stability of the propolis composition can be improved by adding at least one kind of substances selected from polyols, sugars, sugar alcohols and dextrin. The propolis composition contains the active components of both ethanol extract and water extract and is effectively utilizable as a health food.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a propolis composition containing a hydrophilic organic solvent extract of propolis and a water extract which are useful as health foods and the like.

[0002]

2. Description of the Related Art Propolis is a resinous substance collected from plants by bees and is known as a health food material containing various components. Since natural propolis contains foreign substances, an extract obtained by exclusively extracting this is used. As the propolis extract, a component soluble in a hydrophilic organic solvent such as ethanol and a component soluble in water are used.

In the field of health foods, most propolis products are used as an ethanol extract or as a powder, tablet, capsule or the like by adding an excipient to an ethanol extract. It was confirmed that these ethanol extracts contained substances such as flavonoids, phenolic acids, coumarins, and aromatic aldehydes.
It has been reported that these substances have antibacterial and antioxidant effects. Since most of the ethanol extract of propolis is a hydrophobic component, it becomes a tar-like substance when drinking, which makes it difficult to drink. Therefore, some techniques for improving this have been proposed.

For example, Japanese Patent Publication No. 4-66544 proposes a propolis food composition and a method for producing the same. That is, according to this method, a propolis component soluble in a monohydric alcohol or a propolis component soluble in a plurality of hydrogen-bondable hydroxyl group (OH group) -containing media is mixed with a plurality of OH group-containing media and a fatty acid. A propolis composition stably dispersed with an ester surfactant can be obtained.

Further, JP-A-6-197734 discloses another propolis composition and a method for producing the same. According to this method, the dispersibility of propolis in water is improved by including saponin in the ethanol extract of propolis.

Further, Japanese Patent Application Laid-Open No. 5-316968 discloses a purified propolis extract, a method for producing the same, and its use. According to it, the concentration in the hydrophilic organic solvent extraction fraction of propolis was 30 to 55% by volume.
Only the fraction soluble in the solvent (1) is purified and the handling is easy.

On the other hand, a water extraction method is known in which propolis is extracted using water instead of a hydrophilic organic solvent such as ethanol. It has been reported and noted that a substance having an anticancer effect is present in this water extract. As the water extract, a product in the form of a water extract is known.

As described above, a technique has been established in which different components of propolis are extracted with a hydrophilic organic solvent typified by ethanol or water, and an extract containing a unique active ingredient is obtained.

[0009]

However, until now, since these extracts have been used separately, they cannot be said to be compositions containing substantially all soluble components of the propolis drug substance. Therefore, it has not been possible to effectively utilize the components extracted by the hydrophilic organic solvent and the components extracted by water at the same time.

Further, since the hydrophilic organic solvent extract of propolis is difficult to disperse in water, if it is mixed at a high concentration, it becomes a tar-like form, which does not adhere to a container or the like, is separated or precipitated, and is difficult to drink. . Moreover, depending on the concentration of the hydrophilic organic solvent, the water extract causes precipitation because the extracted components are not dissolved. These properties are major problems in terms of product stability and usability.

The present invention has been made by paying attention to the problems of the prior art as described above. An object of the invention is to provide a propolis composition containing substantially all of a hydrophilic organic solvent extract of propolis and an aqueous extract of propolis.

Another object of the present invention is to provide a propolis composition containing a hydrophilic organic solvent extract of propolis and a water extract in a stable form and having excellent water dispersibility. .

[0013]

Means for Solving the Problems In order to achieve the above object, the invention of a propolis composition according to claim 1 contains a hydrophilic organic solvent extract of prepolis and a water extract of propolis. is there.

The invention according to claim 2 contains a hydrophilic organic solvent extract of propolis, a water extract of propolis, and saponin. In addition, the invention according to claim 3 contains at least one selected from the group consisting of polyols, sugars, sugar alcohols and dextrins.

The present invention will be described in detail below. The raw material propolis can be obtained in many production areas such as Brazil, China, Japan, the United States, and Europe, and any production area can be used in the present invention. This propolis is preferably crushed in advance in order to accelerate the extraction of the active ingredient. In this case, since propolis has an adhesive property due to heat, it is desirable to grind it by freeze grinding or cooling grinding.

The hydrophilic organic solvent is for extracting an active ingredient soluble in the hydrophilic organic solvent in propolis, and lower alcohols such as ethanol, methanol and isopropanol which are mutually soluble with water, as well as acetone and methyl ethyl ketone. Ketones and the like are selected and used according to the application. For food applications, ethanol is usually used. The concentration of the hydrophilic organic solvent is preferably 60% by volume or more, and more preferably 80% by volume or more, in order to efficiently extract the components soluble in the organic solvent. The amount of the hydrophilic organic solvent used is at least half the amount of the propolis raw material, preferably at least the same amount.

The hydrophilic organic solvent extract of propolis is a propolis raw material extracted with a hydrophilic organic solvent. As for the extraction conditions, it is important that the solvent sufficiently penetrates into the raw material, and extraction time and appropriate stirring according to the extraction amount are required. Usually, the extraction operation is carried out for 24 hours or longer, preferably 72 hours or longer with stirring at a temperature near room temperature of 10 to 20 ° C. This is because the operation near room temperature has good workability and filterability. In addition, increase the extraction efficiency,
In order to carry out extraction in a short time, it is better to raise the temperature, but the filterability of the extract will be poor. In this case, the filterability can be improved by using a filter aid such as diatomaceous earth.

The hydrophilic organic solvent has good permeability to raw materials,
In addition, since some of them have the property of easily catching fire, in that case, it is desirable to perform extraction without raising the temperature. The obtained extract can be used as it is, but can also be used by diluting it or concentrating it if necessary.

On the other hand, the water extract of propolis is an active ingredient soluble in water extracted from the raw material of propolis with water. The extraction temperature in the water extraction may be room temperature, but is preferably 10 ° C. or higher, particularly preferably 40 ° C. or higher in order to enhance the extraction efficiency and shorten the extraction time. The extraction time depends on the extraction amount, but is preferably 5 hours or more. In this case, it is preferable to perform the extraction operation while stirring. The amount of water as a solvent is preferably 2 times or more the amount of the raw material, more preferably 5 times or more, because the water extraction rate of the active ingredient in propolis is low. This water extract can be used as it is, but can be used after diluting or concentrating it as needed.

The propolis raw materials for obtaining the hydrophilic organic solvent extract and the water extract may be the same or different, but they are the same in consideration of workability of the extraction process and yield of the propolis composition. It is preferable to perform two types of extraction from the raw material. That is, after the propolis raw material is first extracted with a hydrophilic organic solvent, the residue may be subjected to water extraction, or conversely, the residue after water extraction may be subjected to hydrophilic organic solvent extraction. . Among them, it is most preferable to first perform extraction with a hydrophilic organic solvent using the same raw material, and then extract the residue with water. That is, the residue after being first subjected to the hydrophilic organic solvent extraction is changed into a physical property with good workability by the action of the hydrophilic organic solvent, the permeability of the extracted water is improved, and the extraction liquid is also filtered. It will be easy. Further, the generation of tar during the water extraction is suppressed, and the yield of the propolis composition is improved.

The mixing ratio of the two extracts is not particularly limited, but from the viewpoint of containing the complete components of the propolis raw material, it is preferable to match the composition of the raw material. That is, for example, if 40 parts by weight of the hydrophilic organic solvent extract and 10 parts by weight of the water extract as solids can be extracted from 100 parts by weight of a certain raw material, the hydrophilic organic solvent extract and the water extraction can be extracted. The mixing ratio of the solids is 4:
1 is preferred. However, one of the components may be predominantly contained in the product design that places importance on the effective component of either extract, and is not limited.

Next, saponin is for mixing a hydrophilic organic solvent extract of propolis and a water extract of propolis in a stable state, and further for providing excellent water dispersibility at the time of drinking. .

Saponin is a glycoside existing in plants and has a hydrophobic portion and a hydrophilic portion in its molecular structure, and this structure shows so-called surface activity. Saponin
As ginseng saponin and soybean saponin themselves are functional food materials, they are excellent as health food materials as surfactants compared to general chemically synthesized products.

Suitable saponins include querilla saponins, ginseng saponins, soybean saponins, and edgy saponins for foods. The purity of saponin is not particularly limited as long as it has surface activity, but quillaja saponin is particularly preferable because it has excellent surface activity and is commercially available as an emulsifier. The amount of saponin used also varies depending on the concentration and blending ratio of the hydrophilic organic solvent extract of propolis and the aqueous extract of propolis, but is usually the saponin solid content relative to the hydrophilic organic solvent extract solids of propolis. 0.01 to 50% by weight is preferable, and 1 to 20% by weight is more preferable.

Next, at least one selected from the group consisting of polyols, sugars, sugar alcohols and dextrins has a function as an emulsification aid, a stabilizer, an excipient, etc., and enhances the function of saponin to make it more stable. It is what makes it. These polyols, sugars, sugar alcohols, and dextrins are not particularly limited in purity and kind, but it is necessary that the propolis composition has good dispersibility and has an appropriate viscosity. This property makes the propolis composition more stable.

The polyol is a polyhydric alcohol such as glycerin and propylene glycol, and glycerin which can be used in foods without limitation is particularly preferable. As sugar, any of sucrose, fructose, glucose, honey, starch syrup and the like can be used. As the sugar alcohol, sorbitol, maltitol, reduced starch syrup and the like are all used. As the dextrin, cyclodextrin, branched cyclodextrin or the like is used. The amount and concentration of these polyols, sugars, sugar alcohols, and dextrins are not particularly limited, but a concentration containing 0.1 to 10% by weight of the hydrophilic organic solvent extract of propolis is particularly preferable.

Next, the method of mixing the solution of the hydrophilic organic solvent extract and the aqueous solution of the water extract is not particularly limited, but it is preferable to use an apparatus capable of dispersion emulsification such as a propeller stirrer.

The propolis composition may be diluted to a predetermined concentration, concentrated, or added with an excipient such as saccharide to form a paste or powder, if necessary. Further, it may be in any food form such as a liquid drink or a solid tablet.

In the case of powdering, first, the liquid propolis composition described above is concentrated so that the solid content is preferably 10% by weight or more, more preferably 30% by weight or more. Next, 50% by weight or more of excipients such as lactose, dextrin, and rice flour are added to this concentrated liquid, and a hot air drier, a reduced pressure drier,
It is dried at 30 to 80 ° C. for 5 to 24 hours using a freeze vacuum dryer or the like. Then, this dried product is pulverized by a pulverizer or the like to obtain powder of 50 mesh or less.

By the way, the propolis composition of the present invention may be a mixture of the powder of the hydrophilic organic solvent extract and the powder of the water extract. In this case, as a method of pulverizing the solution of the hydrophilic organic solvent extract, first, the extract is concentrated so that the extract concentration is preferably 10% by weight or more, more preferably 30% by weight or more. Then, the concentrated liquid is added with an excipient such as lactose, dextrin, or rice flour, preferably 10
% Or more, more preferably 30% or more.
Then, it is dried at 30 to 80 ° C. for 5 to 24 hours using a hot air dryer, a reduced pressure dryer, a freeze vacuum dryer, or the like. Then, this dried product is pulverized by a pulverizer or the like to obtain powder of 50 mesh or less.

On the other hand, as a method for pulverizing the aqueous solution of the water extract, first, as in the above, the concentration of the extract is preferably 10% by weight or more, more preferably 30% by weight or more. The above-mentioned excipient may be added to this concentrated solution, but a good powder can be obtained without adding it. Thereafter, drying and pulverization are performed in the same manner as pulverization of the organic solvent extract solution.

The two kinds of powder thus obtained are
The powder containing the hydrophilic organic solvent extract and the water extract is obtained by mixing with the powdery or granular material mixer. As described above, the propolis composition of the present invention has a hydrophilic organic solvent extract and a water extract, and since each contains a unique active ingredient, liquid, tablets, capsules,
It is preferably used in the form of powder or the like as a health food or a drug.

[0033]

The propolis composition according to claim 1 contains both active ingredients of a hydrophilic organic solvent extract of propolis and an aqueous extract of propolis, so that a more complete composition of the active ingredient of propolis is contained. Can be provided at.

In the propolis composition according to claim 2,
Since saponin expresses excellent emulsifying power, a stable composition containing a hydrophilic organic solvent extract of propolis and a water extract is obtained, and this composition exhibits excellent water dispersibility.

In the propolis composition according to claim 3,
Since the polyol, sugar, sugar alcohol or dextrin exerts the function as an emulsification aid or a stabilizer, the propolis composition of claim 2 is further stabilized.

[0036]

EXAMPLES The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples. (Example 1, an example of a liquid in which an ethanol extract of propolis and an aqueous extract are mixed) In step 1, 10 kg of a frozen propolis original mass produced in China was pulverized by a pulverizer [Fuji Bowdal Co., Ltd., Pulverizer]. The mixture was pulverized, 200 liters of 95% by volume ethanol was added, and the mixture was extracted at room temperature for 4 days with occasional stirring. After standing for 24 hours, the supernatant was filtered with a filter paper [Advantech Toyo Corp., No. 2] and filtered to give 153 kg of ethanol extract of propolis (solid content 3.0
Wt%) was obtained.

In step 2, 10 kg of a frozen Chinese propolis raw mass was crushed with a crushing surf [Pulverizer manufactured by Fuji Paudal Co., Ltd.].
The mixture was stirred and extracted at 5 ° C. for 15 hours with a stirrer (manufactured by Shimajun Seisakusho Co., Ltd.). 10 kg of diatomaceous earth [manufactured by Chuo Silica Co., Ltd., trade name Silica 300S] was added to the suspension, and the suspension was subjected to diatomaceous earth filtration. As a result, 184 kg of clear propolis water extract
(Solid content 0.41% by weight) was obtained.

As step 3, 30.6 kg of ethanol extract of propolis of step 1 and 36.8 kg of water extract of step 2
Were mixed by stirring with a stirrer (manufactured by Shimazaki Seisakusho). As a result, 67.2 kg of a propolis composition containing an ethanol extract and a water extract of propolis was obtained. The composition contains 1.4% by weight of ethanol extract of propolis and 0.2% by weight of water extract of propolis. (Example of Powder Based on Liquid Concentrated Liquid Obtained in Examples 2 and 1) 30 kg of the propolis composition of Example 1 was concentrated with a concentrator [NE51 manufactured by Tokyo Rika Kikai Co., Ltd.] ．
It was set to 9 kg. 0.9 kg of this concentrate and 3.5 kg of rice flour were mixed with a mixing stirrer (manufactured by Dalton Co., Ltd.). The mixture was dried at 70 ° C. for 12 hours with a hot-air dryer (manufactured by Nichiken Sangyo Co., Ltd.). The dried product was pulverized by a pulverizer [Pulverizer manufactured by Fuji Paudal Co., Ltd.] to obtain 3 kg of a powdery propolis composition containing an ethanol extract of propolis and a water extract of propolis with a 50 mesh path. . (Example 3, an example of a powder obtained by concentrating an ethanol extract of propolis into a powder and mixing an aqueous extract with a water extract into a powder) An ethanol extract of propolis in step 1 of Example 1 30.6 kg was concentrated by a rotary evaporator [NE-51 manufactured by Tokyo Rikakikai Co., Ltd.] to obtain 1.8 kg of concentrated liquid (solid content 51% by weight). Lactose (10 kg) was mixed with this concentrated liquid with a mixing stirrer (manufactured by Dalton Co., Ltd.), and heated at 65 ° C. for 12 hours with a hot-air dryer (manufactured by Netsuken Sangyo Co., Ltd.).
Let dry for hours. Dried product crusher [Fuji Paudal Co., Ltd.
Manufactured by Pulverizer Co., Ltd.] to obtain 10.5 kg of powder of 50 mesh pass.

Next, water extract 3 of propolis of Example 1
6.8 kg was concentrated with a rotary evaporator [Tokyo Rika Kikai Co., Ltd., NE-51] to obtain 0.75 kg of concentrated liquid.
(Solid content 20.1% by weight) was obtained. This concentrated solution was freeze-dried in a freeze-dryer [Okawara Seisakusho SF1OO].
C., and dried for 20 hours. The dried product was pulverized with a pulverizer [Pulverizer, manufactured by Fuji Paudal Co., Ltd.] to obtain 0.15 kg of powder of 50 mesh pass. Further, 10.5 kg of the ethanol extract powder of propolis and 0.15 kg of the water extract powder of propolis were mixed by a mixer [manufactured by Fuji Paudal Co., Ltd.,
V mixer] to mix the powdered propolis composition 44.
3 kg was obtained. (Example 4, an example of a liquid obtained by adding honey to an ethanol extract solution of propolis and an aqueous extract solution) 30.6 kg of the ethanol extract of propolis of step 1 of Example 1 was stirred in 60 kg of honey [manufactured by Shimazaki Seisakusho Co., Ltd. ] By stirring and mixing, and further step 2
36.8 kg of the water extract of propolis of was mixed with stirring. As a result, 127.4 kg of a propolis composition containing 0.7% by weight of an ethanol extract of propolis and 0.08% by weight of an aqueous extract of propolis was obtained. (Examples of liquid-based powders of Examples 5 and 4) 10 kg of the propolis composition of Example 4 was mixed with 100 kg of dextrin [Matsutani Chemical Co., Ltd., Paindex] using a mixing stirrer [Dalton Co., Ltd.]. Then, it was dried at 65 ° C. for 12 hours with a hot air dryer (manufactured by Nichiken Sangyo Co., Ltd.). The dried product was pulverized by a pulverizer [Pulverizer, manufactured by Fuji Paudal Co., Ltd.] to obtain 10 lkg of powder of 50 mesh pass. (Example 6, Example of Propolis Ethanol Extract, Water Extract, and Saponin-Containing Liquid) In Step 1, 10 kg of frozen Brazilian propolis original mass was crushed [Fuji Paudal Co., Ltd., Pulverizer] ] Crush with 80
50% of ethanol was added, and the mixture was stirred and extracted at room temperature for 8 days. After standing for 24 hours, the supernatant was filtered with a filter paper [Advantech Toyo Corp. No. 2] to obtain 33 kg of an ethanol extract of propolis (solid content: 10.6% by weight).

In step 2, 10 kg of frozen Brazilian propolis raw mass was crushed by a crusher (manufactured by Fuji Paudal Co., Ltd.,
Pulverizer], water (100 liters) was added, and the mixture was stirred and extracted at 45 ° C. for 15 hours with a stirrer (manufactured by Shimazaki Seisakusho). Diatomaceous earth in suspension [Chuo Silica Co., Ltd.
Silica 10 OF] was added thereto and the mixture was subjected to diatomaceous earth filtration. As a result, 85 kg of a clear water extract of propolis (solid content: 1.0% by weight) was obtained.

In step 3, 6.6 kg of the ethanol extract of step 1 was added to saponin [Kirayanin C manufactured by Maruzen Pharmaceutical Co., Ltd.].
-100] 500 g was added and mixed with stirring. further,
This mixture was agitated with 17 kg of the water extract from step 2.
As a result, 24 kg of a propolis composition containing an ethanol extract of propolis, a water extract thereof, and saponin was obtained. When 1 g of this propolis composition was directly ingested, it could be ingested without causing tar in the oral cavity. (Examples of Powders Based on Liquids of Examples 7 and 6) 10 kg of the propolis composition of Example 6 was concentrated with a concentrator [NE51, manufactured by Tokyo Rika Kikai Co., Ltd.] to give a concentrate of 1.5 kg. Lactose (7 kg) was mixed with this concentrated liquid by a mixing stirrer (manufactured by Dalton Co., Ltd.). The mixture was dried at 70 ° C. for 12 hours with a hot-air dryer (manufactured by Nichiken Sangyo Co., Ltd.). 1. The dried product was crushed with a crusher [Pulverizer, manufactured by Fuji Paudal Co., Ltd.], and a propolis composition of a powder containing ethanol extract of propolis and water extract of propolis and saponin of 50 mesh pass. 1 kg was obtained. (Example 8, an example of a liquid obtained by extracting the residual liquid after water extraction with ethanol, and adding saponin and reduced starch syrup) In step 1, 1 kg of a frozen propolis original propolis mass from Uruguay was crushed by a pulverizer [Fuji Paudal (stock) Manufactured by Shimazaki Seisakusho Co., Ltd.) and added with 10 liters of water. The supernatant is filtered with a filter paper [Advantech Toyo Corp., No. 2] to obtain 8.3 kg of a filtrate. 100 g of diatomaceous earth [Silica 10OF manufactured by Chuo Silica Co., Ltd.] was added to the filtrate and the mixture was subjected to diatomaceous earth filtration. As a result, clear propolis water extract 8. 1 kg (solid content 1.1% by weight) was obtained.

As the step 2, 95% is added to the extraction residue of the step 1.
Ethanol (3 liters) was added, and the mixture was stirred and extracted at room temperature for 24 hours. After standing for 24 hours, the supernatant was filtered with a filter paper [Advantech Toyo Corp., No. 2] to obtain an ethanol extract of propolis (1.9 kg, solid content: 14% by weight).

In step 3, 1.9 kg of the ethanol extract of propolis in step 2 was added to saponin [Maruzen Pharmaceutical Co., Ltd.,
100 g of Kirayanin C-100] was added and mixed with stirring with a stirrer (manufactured by Shimazaki Seisakusho Co., Ltd.) to obtain a mixed solution of 2.0 kg.
I got 2.0 kg of this mixed solution was stirred and mixed with 20 kg of reduced starch syrup [SE600 manufactured by Niken Chemical Co., Ltd.], and the water extract of propolis in step 1 was used. 1 kg was mixed with stirring.
As a result, 30 kg of a propolis composition containing an ethanol extract and a water extract of propolis was obtained. This composition showed excellent water dispersibility. (Examples of powders obtained by adding lactose to the liquids of Examples 9 and 8)
60 kg of lactose was mixed with 10 kg of the propolis composition of Example 8 using a mixing stirrer (manufactured by Dalton Co., Ltd.). The mixture was dried at 70 ° C. for 12 hours with a hot-air dryer (manufactured by Nichiken Sangyo Co., Ltd.). The dried product is crushed by a crusher [Fuji Paudal Co., Ltd., Pulverizer], and an ethanol extract of propolis of 50 mesh path, an aqueous extract of propolis, a saponin, a reduced starch syrup and a powdered propolis composition containing lactose 64
kg gained. (Example 10, Example of liquid in which saponin-added liquid was added to ethanol extract, glycerin, and water extract from residual liquid after ethanol extraction) As step 1, frozen propolis original mass of Brazil 1 kg was pulverized with a pulverizer [Fuji Paudal Co., Ltd., palberizer] to obtain 90% by volume.
Ethanol (8 liters) was added, and the mixture was stirred and extracted at room temperature for 4 days. After standing for 24 hours, the supernatant was filtered with a filter paper [Advantech Toyo Corp. No. 2] and then filtrate 5.7kg
I got 5.7 kg of this filtrate was concentrated under reduced pressure to obtain 1.8 kg of ethanol extract of propolis (solid content 21% by weight).

In step 2, 5 liters of water was added to the extraction residue of step 1, and the mixture was stirred and extracted at 45 ° C. for 13 hours with a stirrer (manufactured by Shimazaki Seisakusho). 1 kg of diatomaceous earth [Silica 600S, manufactured by Chuo Silica Co., Ltd.] was added to the suspension, and the suspension was subjected to diatomaceous earth filtration. As a result, 4.3 kg of a clear water extract of propolis (solid content: 1.1% by weight) was obtained.

In step 3, 1.8 kg of ethanol extract of propolis in step 1 was added to saponin [manufactured by Maruzen Pharmaceutical Co., Ltd.,
200 g of Kirayanin C-100] was added and mixed by stirring with a stirrer to obtain 2.0 kg of a mixed solution. 3. This mixture was stirred and mixed with 5 kg of glycerin [food grade glycerin manufactured by Asahi Denka Kogyo Co., Ltd.], and the water extract of propolis in step 2 was used.
Stir-mixed to 3 kg.

As a result, 11.2 kg of a propolis composition containing an ethanol extract and a water extract of propolis was obtained. When 10 g of this propolis composition was diluted with 50 ml of water, it showed good water dispersibility. (Example of a liquid obtained by repeating the concentration and water addition of the liquids of Examples 11 and 10 to remove ethanol) 5 kg of the propolis composition prepared in Example 10 was applied to a rotary evaporator [Tokyo Rika Kikai Co., Ltd., NE51]. It was subjected to concentration.
By repeating concentration and water addition 5 times, ethanol was removed from the components. As a result, 3 kg of a propolis composition containing no ethanol was obtained. In this composition, even a person with a constitution showing a rejection reaction to ethanol could ingest propolis without any problem. (Example in which the liquid of Examples 12 and 10 was used as a drink) A drink was prepared using the propolis composition of Example 10. 2 g of the propolis composition, honey, acidulant, and vitamins were added to 100 ml of a drink, and the mixture was heat-sterilized. This drink had no precipitation and exhibited stable properties in which the propolis component was well dispersed.

The technical idea understood from the above embodiment will be described below. (1) The propolis composition according to claim 1, wherein the hydrophilic organic solvent extract of prepolis is a hydrophilic organic solvent solution, and the water extract of propolis is an aqueous solution. With this configuration,
A stable liquid propolis composition containing a hydrophilic organic solvent extract of propolis and an aqueous extract of propolis is obtained. (2) The propolis composition according to claim 1, wherein both the hydrophilic organic solvent extract of prepolis and the water extract of propolis are powders. According to this structure, a powdery propolis composition containing the hydrophilic organic solvent extract of propolis and the water extract of propolis can be obtained. (3) A method for producing a propolis composition powder, which comprises mixing a hydrophilic organic solvent extract of propolis and a water extract of propolis, concentrating the mixture, adding an excipient, and then drying. According to this manufacturing method, the powder of the propolis composition can be easily manufactured. (4) The method for producing a propolis composition powder according to (3) above, wherein the excipient is lactose, dextrin or rice flour. According to this method, the propolis extract can be pulverized more smoothly. (5) Propolis which is a mixture of the hydrophilic organic solvent extract of propolis and the water extract after the raw propolis raw material is crushed and then extracted with the hydrophilic organic solvent extract. A method for producing a composition. According to this method, a propolis composition containing both extracts can be efficiently obtained. (6) A method for producing a propolis composition, wherein the active ingredient in the raw material of propolis is extracted with a hydrophilic organic solvent, the residue is further extracted with water, and both extracts are mixed. According to this method
The propolis composition can be economically produced while preventing the generation of tars. (7) The propolis composition according to claim 2, wherein the saponin is Quillaja saponin. According to this structure, the water dispersibility and stability of the propolis extract can be improved. (8) The mixing ratio of the hydrophilic organic solvent extract of prepolis and the water extract of propolis is the ratio of the amount of the hydrophilic organic solvent extracted and the amount of water extracted in the raw material propolis.
A propolis composition according to item 1. With this configuration, a propolis composition containing substantially all of the hydrophilic organic solvent extract and the water extract in the prepolis can be obtained.

[0048]

As described in detail above, according to the invention of the propolis composition according to claim 1, a propolis composition containing substantially all of the hydrophilic organic solvent extract of propolis and the water extract of propolis. You can get things.

According to the second aspect of the invention, there is provided a propolis composition containing a hydrophilic organic solvent extract of propolis and a water extract in a stable form and having good water dispersibility. Obtainable.

Further, according to the invention described in claim 3,
A propolis composition containing a hydrophilic organic solvent extract of propolis and a water extract in a more stable form and having excellent water dispersibility can be obtained.

Claims (3)

[Claims] 1. A propolis composition containing a hydrophilic organic solvent extract of prepolis and a water extract of propolis. 2. A propolis composition containing a hydrophilic organic solvent extract of propolis, an aqueous extract of propolis, and saponin. 3. At least one selected from the group consisting of polyols, sugars, sugar alcohols and dextrins.
The propolis composition of claim 2, which comprises a seed.