JPH08302396A - Detergent for contact lens and method for cleaning and sterilizing using the same - Google Patents

Abstract

PURPOSE: To enable the sterilization by boiling of a contact lens immediately after cleaning it without affecting it by using a stable liq. detergent contg. protease and a polycarboxylic acid salt. CONSTITUTION: A polycarboxylic acid supplements and enhances the cleaning power of protease and contributes to the stability of the enzyme in a liq. A citric acid salt, a tartaric acid salt, and a malic acid salt are pref. examples of the acid in terms of safety for a living body. The concn. of the acid is pref. 0.01-2%. Protease in a liq. is stabilized by using a polyol (e.g. sorbitol or glycerin) and a borate. A surfactant (e.g. a nonionic surfactant such as a polyoxyethylene polyoxypropylene alkyl ether or a polyoxyethylene hardened castor oil) is added for improving the cleaning power to oily dirt and for preventing redeposition of dirt. The surfactant is pref. a nonionic one having an HLB value of 13-18 and a mol.wt. of 2,000-3,000 and is used pref. in a concn. of 0.001-0.4W/V%.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cleaning, disinfecting and preserving contact lenses, and a contact lens cleaning agent, especially a soft contact lens cleaning agent, which is necessary to properly carry out these methods.

[0002]

2. Description of the Related Art To clean a contact lens, a protein stain or a lipid stain on the surface of the contact lens is emulsified and decomposed by a method using a detergent containing a surfactant as a main component, or by immersion washing in an enzyme agent. In particular, the washing method using an enzyme has been confirmed to be useful, and powders, granules, tablets or concentrated liquid enzyme preparations are diluted and dissolved with a preservative solution or a rinse solution before use. As the enzyme, proteolytic enzyme, lipolytic enzyme, polysaccharide degrading enzyme, lysozyme and the like are used. These techniques are disclosed in, for example, Japanese Patent Publication No. 53-47810, a washing method using a composition containing an effective amount of a protease and a detergent containing a specific protease such as papain. JP-A-62-913 discloses a detergent comprising a specific enzyme such as amylase or cellulase and an activator such as urea or amino acid.
Japanese Unexamined Patent Publication (Kokai) No. 53-125412 discloses a detergent using lipase.

On the other hand, contact lenses are generally stored in a specific storage solution after cleaning or disinfection.
Hard contact lenses are stored in a storage solution containing a hydrophilizing agent and the like. For soft contact lenses, they are stored or boiled in a physiologically osmotic and isotonic preservative solution containing sodium chloride as a main component, and also used as a rinse solution after washing. Since the preservation solution for soft contact lenses substantially comes into contact with the eye along with the contact lens, isotonicity and safety are important. Therefore, since it is generally prepared separately from the diluent for the cleaning agent, the user has to have three kinds of solutions including the cleaning agent, which is inconvenient. In addition, a proteolytic enzyme is mainly used as a detergent, and a method of heating to activate the proteolytic enzyme and a method of using an activator are adopted. Although metal ions and SH compounds are used as activators, they are not preferable because of problems such as adhesion of activators to contact lenses and yellowing of contact lenses.

[0004]

In the prior art, in order to increase the activity of the detergent, a method of adding an SH group activator such as cysteine or a metal ion such as calcium as an activating component is used. The activator has a problem because it causes yellowing of the contact lens due to the formation of a sulfide bond, and calcium causes water insolubilization and stain deposit of the protein on the contact lens, which is particularly accelerated by heating. In order to avoid such a problem, generally, after washing and rinsing with an enzyme agent or a cleaning agent, it is sterilized by boiling with physiological saline or a preservative solution, but the operation is complicated and the contact lens is damaged. It is also one of the reasons why the risk of loss is high. Some products have improved some of these problems, but they are not perfect. For example, there is a simple one that can be used as both a diluting solution and a preservative disinfectant solution, or one that has improved cleaning performance by adding a surfactant to the diluting solution. However, if a surfactant is added to the diluting solution, the diluting solution and the preserving solution must be set separately, which presents a technical problem.

Therefore, the present invention has been achieved through intensive research aimed at solving these problems and providing a comprehensively superior cleaning agent.

[0006]

[Means for Solving the Problems] Technical means for solving the above problems, that is, enhancing the detergency without affecting contact lenses, and further improving biological safety and stability of the detergent. It is to find a means that does not damage it. As a result of diligent research based on this idea, we found a polycarboxylic acid that is safe and has little effect on contact lenses as a detergency-activating component, and that this component is effective within a specific concentration range. It was found that the activation of power was increased by heating. In addition, this polyvalent carboxylic acid did not affect the stability of the detergent, especially the protease, but showed the effect of enhancing the stability. Improving the cleaning power by heating is very useful in the case of a care method in which cleaning and boiling disinfection of soft contact lenses is performed in one step, and reliable cleaning and disinfection of soft contact lenses can be easily performed.

The present invention has been completed on the basis of such findings, and (1) is a stabilized liquid detergent containing a proteolytic enzyme and a polyvalent carboxylic acid salt. Cleaning agent, (2) polyvalent carboxylic acid salt is at least one of citrate, tartrate, malate
Cleaning agent for contact lenses, characterized by being a seed
(3) 0.01 to 2 W / V% of polyvalent carboxylic acid salt is contained in a detergent for contact lenses, (4) 0.01 to 4 W of polyoxyethylene polyoxypropylene alkyl ether type surfactant 0.01 to 4 W / V% of a cleaning agent for contact lenses, characterized by containing / V%, (5) polyoxyethylene hydrogenated castor oil type surfactant
A cleaning agent for contact lenses, characterized in that
(6) The contact lens is immersed in a mixed solution prepared by diluting the cleaning agent for contact lenses according to claim 1, and the temperature is set to room temperature or 4
A method for cleaning a contact lens, which comprises performing a warm cleaning at 0 to 75 ° C. (7) Immersing the contact lens in a mixed solution diluted with the cleaning agent for a contact lens according to claim 1, and at room temperature or 40 to A method for cleaning a contact lens, which comprises cleaning by heating at 75 ° C. and then sterilizing by boiling, (8) immersing the contact lens in a mixed solution diluted with the cleaning agent for a contact lens according to claim 1. A cleaning method for contact lenses is characterized in that after cleaning by heating at room temperature or at 40 to 75 ° C., it is replaced with a storage solution for contact lenses and sterilized by boiling.

More specifically, the present invention is characterized in that a proteolytic enzyme and a polyvalent carboxylate are included as essential components of a detergent for contact lenses, and the effect is that the polyvalent carboxylate is a proteolytic enzyme. That is, the detergency is remarkably supplemented to synergistically enhance the detergency, and the polyvalent carboxylate contributes to the stability of the proteolytic enzyme in the liquid. Of the polyvalent carboxylates, citrate, tartrate, and malate are preferable and most preferable in terms of safety for living bodies. Citrate and malate are important components in the living body and have an important role in the TCA cycle (Krebs cycle) of the metabolic system circuit.

Further, it is used as an anticoagulant, a flavoring agent for granules and the like, and an effervescent component of tablets as a drug, and is used as clomiphene, diethylcarbamazine, pentoxyberine, etc. as a salt of a drug and its safety is high. high. The concentration of the polycarboxylic acid salt is preferably 0.01 to 2%. If it is less than 0.01%, no improvement in detergency is observed, and if it exceeds 2%, no improvement in detergency is observed, and the shape of the contact lens is affected. More preferably 0.05 to 0.5%
The range is good. The detergency activating action of the polyvalent carboxylic acid according to the present invention is different from conventional proteolytic enzyme activators,
It is considered that the catalytic action that enhances the activity of proteolytic enzyme is not the main action, but the ionic action of the carboxyl group makes it easier to remove stains and at the same time creates an environment where the proteolytic enzyme is likely to act.

For liquid stabilization of proteolytic enzymes, polyols such as sorbitol and glycerin and borate are generally used. Stabilization of proteolytic enzymes by these stabilizers is excellent and has already been put to practical use, but it is also possible to add a nonionic polymer component or a carboxylate such as acetate to further increase the stability. Proposed. However, the stability due to the nonionic polymer cannot be expected so much, and the stabilization due to the carboxylic acid salt has little effect, and there is a problem of a peculiar odor, which is not preferable. The polycarboxylic acid salt of the present invention is characterized in that it improves storage stability of proteolytic enzymes, dramatically improves detergency, is highly safe without odor problems, and does not adversely affect contact lenses. It is important to have

The contact lens cleaning agent of the present invention may contain a surfactant for cleaning the oily greasy dirt and preventing the redeposition of the dirt. The surfactant is preferably a nonionic surfactant from the viewpoint of influence on contact lenses and biological safety. Among the nonionic surfactants, those which do not affect the contact lens, do not affect the stability and action of the protease, and have high biological safety are selected. In the present invention, it has been found that polyoxyethylene polyoxypropylene alkyl ether and polyoxyethylene hydrogenated castor oil are suitable as surfactants that meet this condition. These surfactants are PBC
-34, PBC-44, HCO40, HCO60, HC
It is commercially available under the trade name of O100 (Nikko Chemicals Co., Ltd.) and can be easily obtained. Further, the HLB value (hydrophilic-hydrophobic balance value) is preferably 13-18,
The molecular weight is 800 to 4000, more preferably 2000 to
It is 3000. The concentration of the surfactant is 0.01 to
4 W / V% is preferable. If it is less than 0.01 W / V, it does not function as a surfactant, and if it exceeds 4 W / V%, it is not preferable in terms of solubility and influence on contact lenses, and the cleaning property is not improved. Actually, the contact lens cleaning agent of the present invention is used by diluting it with physiological saline or a dedicated diluting solution or the like, and therefore cannot be unconditionally limited, but the concentration after dilution is 0.001 to 0.4 W / V%. It is generally used in a range.

The contact lens cleaning agent of the present invention is used by dissolving and diluting it with physiological saline or a dedicated diluent, but it is most preferable that it is closer to physiological osmotic pressure after preparation. Specifically, it is preferably 190 to 350 mmol / l, more preferably 260 to 330 mmol / l. If it deviates from this range, the shape of the contact lens may change, stains and cleaning agent components may be adsorbed, which is not preferable. The pH after preparation is preferably 6 to 8, and physiologically unfavorable if the pH is out of this range. Further, if the pH value shifts extremely, lens deformation and polymer chain breakage occur, which is not suitable.

The cleaning method of the present invention comprises immersing a contact lens in a solution obtained by diluting a contact lens cleaning agent,
A high cleaning effect appears by cleaning at room temperature or under heating. In particular, the present invention is characterized in that the cleaning effect is further improved by heating. Generally, detergents containing enzymes increase the washing efficiency by heating,
In the present invention, the coexistence of the polycarboxylic acid salt has been shown to dramatically improve the cleaning effect. Therefore, a small amount of enzyme can ensure high detergency, which may provide a product that can be used even for users who have an enzyme stimulus. The heating temperature is preferably 40 to 75 ° C, more preferably 40 to 60 ° C. According to the present invention, it is possible to provide a safe and efficient method for cleaning a contact lens.

In the cleaning and disinfecting method of the present invention, the contact lens is immersed in a solution obtained by diluting the contact lens cleaning agent, and after cleaning at room temperature or under heating, the temperature is continuously raised to about 100.
The object of the present invention can be achieved by raising the temperature to around 0 ° C to sterilize by boiling, or by once replacing the cleaning solution with a storage solution and then sterilizing by boiling.

Therefore, by using the cleaning agent for contact lenses of the present invention, more efficient cleaning of contact lenses, cleaning and disinfection that does not affect the contact lenses can be ensured to the user after ensuring biological safety. There are advantages that can be provided.

In the present invention, conventionally known components can be used within a range that does not affect the contact lens.
That is, inorganic salts such as sodium chloride and potassium chloride for maintaining an appropriate osmotic pressure, metal chelating agents such as ethylenediaminetetraacetic acid salt capable of chelating and removing metal ions such as calcium ions and iron ions, phosphoric acid Buffering agents such as salts and borates can be included.

[0017]

EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited thereto. Example 1 Proteolytic enzyme (Novo Nordisk Bioindustry Japan KK, Subtilisin A) 1 g, boric acid 1 g, malic acid disodium salt 1.0
g and 30 g of glycerin were dissolved in 70 g of distilled water to obtain the detergent for contact lenses of the present invention. The pH after preparation is 5.
It was 8. 1 drop (about 35 mg) of this cleaning agent was dissolved in about 2.4 ml of Super Bio Step 2 liquid (manufactured by Seiko Contact Lens Co., Ltd.), and a soft contact lens (manufactured by Seiko Contact Lens Co., Ltd., Seiko Soft CSI) was put therein, and 45 Cleaning and boiling disinfection were performed using a boiling disinfecting device Microbiolyzer (manufactured by Seiko Contact Lens Co., Ltd.) having a heating step of 20 minutes at 0 ° C and a boiling disinfection step of 15 minutes at 100 ° C. After cooling, it was rinsed with the Super Bio Step 2 liquid used in this example. This operation was repeated 100 times. In each case, no change in the base curve and discoloration of the soft contact lens was observed, and no change in the ultraviolet transmittance was observed, and there was no effect on the contact lens. In addition, wash and boil disinfect with the same combination of solutions using a soft contact lens with artificial protein stains attached, and after cold rinsing with distilled water in a beaker, use a slit lamp (Carl Zeiss Co., Ltd.) in the dark room. The degree of adhesion of dirt was judged. As a result, protein stains were almost completely removed (rank A).

<Method for producing soft contact lens with artificial protein stain> 0.4 g bovine serum albumin, 0.2 g bovine serum gamma globulin, 0.2 g egg lysozyme, 0.05 g mucin derived from pig stomach, 100 ml of physiological saline. The solution was suspended and dissolved, and the insoluble material was filtered through filter paper (TOYO No. 1). 25 ml of this dirt solution was put in a 200 ml beaker, a soft contact lens was immersed therein, denatured and adhered at 65 ° C. for 30 minutes, and dried at 50 ° C. for 30 minutes. Do this operation 3
Repeatedly, soft contact lenses were produced with artificial protein stains in which white proteins were adhered to the entire surface of the soft contact lenses.

<Evaluation Criteria for Detergency> A There is no cloudiness of the lens B There is a slight cloudiness of the lens C The cloudiness of the lens is 20% or more of the total D The cloudiness of the lens is 50% or more of the total E The cloudiness of the lens is 75% or more of the whole F lens is clouded over the entire surface (Examples 2 to 5) A cleaning solution for contact lenses was prepared in the same manner as in Example 1, and the same test was performed. As a result, as shown in Table 1, the base curve of the contact lens was at an allowable level (within ± 0.2 mm), there was no discoloration deformation and no change in ultraviolet transmittance, and the detergency was sufficiently satisfactory. The formulation and test results are shown in Table 1.

[0020]

[Table 1]

Example 6 The contact lens solution of Example 1 was used to compare the cleaning efficacy with the cleaning temperature. Washing temperature is 30 ℃, 40 ℃, 50 ℃, 60 ℃, 75 ℃, 8
The cleaning was performed at 0 ° C. and 6 levels, and the cleaning time was 30 minutes. The evaluation was according to Example 1 (shown in Table 2).

As a result, the cleaning effect at 30 ° C. was insufficient, but it was satisfactory between 40 ° C. and 75 ° C. Further, at 80 ° C, the cleaning effect was lowered. It is considered that this is because the enzyme was inactivated by heat denaturation of the enzyme and the artificial soil protein was denatured before being decomposed.

[0023]

[Table 2]

Comparative Examples 1 to 5 Cleaning solutions for contact lenses were prepared in the same manner as in Example 1 and the same tests were conducted. As a result, as shown in Table 3, in Comparative Example 1, the change in the base curve is large and the entire contact lens is swollen, which is not suitable. In Comparative Example 2, the change in the base curve was large because of the large amount of the surfactant, and the shape was changed to a slightly elliptical shape, and the decrease in the ultraviolet transmittance was also recognized. In Comparative Example 3, the contact lens was not affected, but the detergency was insufficient. In Comparative Examples 4 and 5, discoloration of contact lenses and a decrease in ultraviolet transmittance were remarkably observed, which were not suitable.

[0025]

[Table 3]

[0026]

EFFECTS OF THE INVENTION The present invention dramatically improves the cleaning effect by containing 0.01 to 2% of highly safe polyvalent carboxylic acid salt as a cleaning activating component according to claims 1, 2 and 3. In particular, the cleaning ability of a cleaning agent using an enzyme can be effectively exerted. According to the fourth and fifth aspects, the inclusion of the specific surfactant can further improve the detergency without affecting the contact lens.
Further, according to claims 6, 7 and 8, the cleaning effect can be enhanced by heating to 40 to 75 ° C, and after cleaning without affecting the contact lens with a more suitable detergent composition, it is then boiled. Since it can be sterilized, contact lens care can be performed easily and easily.

──────────────────────────────────────────────────続 き Continuation of the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display location G02C 13/00 G02C 13/00

Claims (8)

[Claims] 1. A cleaning agent for contact lenses, which is a stabilized liquid cleaning agent containing a protease and a polycarboxylic acid salt. 2. The cleaning agent for contact lenses according to claim 1, wherein the polycarboxylic acid salt is at least one selected from citrate, tartrate and malate. 3. A polyvalent carboxylic acid salt of 0.01 to 2 W / V%
The cleaning agent for contact lenses according to claim 1, which comprises: 4. A polyoxyethylene polyoxypropylene alkyl ether type surfactant in an amount of 0.01 to 4 W / V%.
The cleaning agent for contact lenses according to claim 1, which comprises: 5. The cleaning agent for contact lenses according to claim 1, which contains 0.01 to 4 W / V% of a polyoxyethylene hydrogenated castor oil type surfactant. 6. A method for cleaning a contact lens, which comprises immersing the contact lens in a mixed solution obtained by diluting the cleaning agent for contact lenses according to claim 1, and heating the contact lens at room temperature or at 40 to 75 ° C. while heating. 7. A contact lens is immersed in a mixed solution obtained by diluting the cleaning agent for contact lenses according to claim 1, washed by heating at room temperature or 40 to 75 ° C., and then sterilized by boiling. How to clean and disinfect contact lenses. 8. A contact lens is immersed in a mixed solution prepared by diluting the detergent for contact lenses according to claim 1, washed by heating at room temperature or at 40 to 75 ° C., and then replaced with a preservation solution for contact lenses and boiled. A method for cleaning and disinfecting a contact lens, which is characterized by disinfecting.