JP3079553B2 - Stain remover for contact lenses - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a stain removing agent for a contact lens, and more particularly to a novel cleaning agent having a useful effect for removing stains attached to the surface of a contact lens.

Conventionally, a method using a protease to remove dirt attached to the surface of a contact lens has been widely used.However, it has been recognized that the activity of the protease is unstable when it is in a liquid state and its activity is reduced. ing.

The present invention has been made as a means of keeping proteolytic enzymes stable for a long time in liquid form as a result of intensive studies based on the above circumstances, and provides a contact lens stain remover which can be used as a washing / preserving solution.

[Conventional technology]

Conventionally, hard contact lenses whose main component is methyl methacrylate and soft contact lenses made of 2-hydroxymethacrylate or N-vinylpyrrolidone have been widely used as contact lenses. But,
Some complications due to insufficient supply of enzymes to the cornea of the eye have been pointed out with contact lenses made of methyl methacrylate, and today, contact lenses having higher safety and higher enzyme permeability are becoming mainstream. These components contain an organic silane compound or a compound containing fluorine.

However, these high enzyme permeable contact lenses have a drawback that they are easily contaminated when worn for a long period of time, and that they are easily broken and difficult to clean as compared with conventional hard contact lenses. Therefore, when worn, the patient may suffer from clouding of contact lenses due to contamination by lipids, proteins, mucins, bacteria, and cosmetics in tears, resulting in poor vision and damage to eyes.

Lipid stains on the surface of contact lenses can be removed with surfactants contained in general detergents for contact lenses, but proteins and mucins cannot be easily removed, and they remain on the contact lenses and can be used as is If the process is continued, the denatured coagulation proceeds to further firmly adhere, shortening the life of the contact lens.

On the other hand, there has been conventionally known a cleaning agent containing a chemical having an oxidizing action such as a protease, a polysaccharide-degrading enzyme, a lipolytic enzyme, or hypochlorous acid for cleaning contaminated contact lenses.

For example, Japanese Patent Publication No. 53-47810 discloses a washing method using a composition containing an effective amount of a protease and a detergent comprising a specific protease such as papain. JP-A-62-913 proposes a detergent comprising a specific enzyme such as amylase or cellulase and a specific activator such as urea or amino acid. In addition, JP
-125412 discloses a detergent using a lipolytic enzyme such as lipase. Japanese Patent Publication No. 1-32962 discloses a composition using sodium hypochlorite or further adding a specific sugar or acid.

Any of these disclosed cleaning agents can easily remove stains on the contact lens surface by infiltration, so that an effective effect can be expected.

[Problems to be solved by the invention]

However, among the methods described above, Japanese Patent Publication No. 53-4781
No. 0, JP-A-62-913 and JP-A-53-125412, and currently commercially available detergents containing an enzyme as a main component, there are almost no exceptions to enzyme-containing washing solutions prepared according to the disclosed formulation. Without enzyme activity within 24 hours. Therefore, storage in a dry tablet was required to keep the activity high. In this case, the patient needs to dissolve the solid in the dedicated liquid each time it is used, and there is a problem that deformation occurs due to infiltration of the lens in an insufficiently dissolved state.

Further, in the case of the treatment agent using a chemical substance disclosed in Japanese Patent Publication No. 1-32962, the toxicity of the chemical substance itself is high, so that if the rinse agent is not sufficiently rinsed, there is a concern that the treatment solution may cause damage to the eyes.

Therefore, the present invention has been achieved by intensive research and development with the object of solving these problems.

That is, an object of the present invention is to provide a solution type contact lens stain remover which is low in toxicity, has excellent cleaning effect, has excellent long-term storage stability even in solution form, and can be used more easily for patients. The purpose is to do so.

[Means for solving the problem]

The present invention has been found to achieve the object by using a protease and a solvent composition under certain conditions. In particular, the combination of a polyol, a boron compound and an ascorbic acid derivative provides excellent stability of the protease in the liquid soil remover of the present invention, and Mg ion enhances its effect.

The protease used in the present invention is preferably derived from a microorganism. For example, esperase derived from the genus Bacillus, bioprase AP-L, bioprase NY, subtilisin, protease N, and the like are commercially available.

The detergent of the present invention is desirable in that a composition containing 0.01 to 10% by weight, preferably 0.1 to 5% by weight of the enzyme component can obtain a sufficient effect. If the content is less than 0.01% by weight, a sufficient effect cannot be obtained, and if it is more than 10% by weight, the solubility is poor.

The water-miscible polyol used in the present invention has high safety to the living body and preferably does not affect the material of the contact lens.For example, ethylene glycol, propylene glycol, butanediol, pentanediol, hexanediol, diethylene glycol,
Triethylene glycol, tetraethylene glycol,
And polyhydric alcohols such as polyethylene glycol, and polyhydric alcohol sugars such as glycerin and erythritol; and sugar-derived polyhydric alcohols such as glucose, fructose, sorbitol, xylitol, and ascorbic acid. If this polyol is used in an amount of 40% by weight or less, the effect of addition is ineffective, and if it is 95% by weight or more, the solubility of enzymes and other components is poor, and precipitation may occur during storage. Preferably it is 60-95% by weight, but it is limited by the kind of polyol used. The polyols used may be used alone or in combination of two or more, and may further contain ethyl alcohol, isopropyl alcohol, and alkoxy alcohol (cellosolve) which are generally freely mixed with water.

The boron compound used in the present invention is selected from, for example, boric acid, borax and the like. This boron compound has no effect when used alone, but imparts stability to the protease when coexisting with a certain amount of polyol. The amount of the boron compound used is 0.01-1% by weight, especially 0.05-0.
5% by weight is preferred. When the content is 0.01% by weight or less, the effect is hardly exhibited, and when it is 1% by weight or more, it is not preferable in terms of safety to eyes.

The ascorbic acid derivative used in the present invention is preferably stable in a neutral to weakly alkaline aqueous solution.
Mg salts are preferred. For example, there are magnesium ascorbic acid phosphate and the like. This ascorbic acid derivative improves the stability of proteolytic enzymes, and
The ions further increase the stability.

The ascorbic acid derivative used in the present invention obtains the stability of the protease over a longer period of time by adding it to a polyol and boron compound stable system.

The amount of the ascorbic acid derivative used in the present invention is 0.01
-1% by weight, especially 0.03-0.2% by weight is preferred. Also, no effect is exhibited if the amount is too large or too small.

The Mg ion used in the present invention is preferably derived from an inorganic or organic salt, and is selected from, for example, magnesium chloride, magnesium phosphate, magnesium citrate, magnesium ascorbic acid phosphate, and the like.

The amount of Mg ion used in the present invention is 0.001-0.02 mEq /
In particular, 0.005 to 0.01 mEq / is preferable. Mg ions
At a dilute concentration of 0.0001 mEq / or less, there is no stabilizing effect or detergency enhancing effect. On the other hand, if the amount is too large, the stabilizing effect is reduced, and furthermore, it may cause coagulation of proteins and the like at the time of washing, which is not preferable.

The stain remover of the present invention can obtain a given effect by adding some conventionally known components in addition to these essential components. That is, a surfactant can be included to remove lipids. Examples of the surfactant include sulfonates such as sodium sulfonate of polyethers, anionic surfactants including carboxylate and phosphate, polyoxyethylene skeleton, and glycerin fatty acid ester. Nonionic surfactants, cationic surfactants such as quaternary amine salts, and polysaccharide-based surfactants can be used. In addition,
It may contain preservatives, chelating agents, stabilizers, pH adjusters, neutral inorganic salts, thickeners and the like. In order to make the stain remover substantially isotonic with tears, 0.8-1.0% of sodium chloride is used.
It is desirable to add by weight.

Since the stain remover of the present invention is a solution-type stain remover obtained as described above, at the time of use, put 1 to 3 drops in a lens case, and then add about 2 ml of physiological saline. Then, the lens is diluted with distilled water, a specific contact lens preservation solution or the like, and the lens is immersed in the diluent for cleaning. The effect of the cleaning process can be obtained from 15 minutes, but the cleaning is completed in 30 minutes to 2 hours. The cleaning temperature is not particularly limited, but is preferably room temperature or higher. After the treatment, the contact lens can be rinsed with distilled water or physiological saline and can be mounted.

(Operation)

The cleaning solution according to the present invention comprises a specific enzyme, a polyol and a solvent containing a specific amount of an ascorbic acid derivative, and can efficiently decompose and remove dirt on the contact lens surface by hand without the action of the enzyme. In addition, the activity is maintained by chemically protecting the enzyme by the action of the polyol, the boron compound, and the ascorbic acid derivative.

〔Example〕

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto. In addition, in an Example, a part represents a weight part.

Example 1 Glycerin 40 Diethylene glycol 40 Boric acid 0.1 Ascorbic acid phosphate ester magnesium salt 0.1 Bioprase NY 2 Sorbic acid 0.1 Sorbitol 1 Distilled water Total 100 ml <Evaluation criteria> (a) Preparation of a soiled contact lens sample Protein stains were applied to the contact lenses using a stain solution. That is, 200ml
In a beaker, dissolve 0.6 parts albumin, 0.3 parts globulin, 0.2 parts egg white lysozyme, 0.1 part rutin in physiological saline
It was 100 ml. Next, the well-washed contact lens was immersed, and the soil solution was heated to 65 ° C. to denature the protein and adhere to the contact lens surface. The obtained contact lens was dried at room temperature and used as a test sample. The stain of the sample thus obtained was such that it did not fall off even if it was rubbed by hand.

(B) Detergency evaluation test Commercial storage case for contact lenses (capacity 2.5 ml)
Then, 0.1 part of the enzyme washing solution obtained in this example was added, and 1.9 parts of physiological saline was added dropwise. As a result, the enzyme washing solution immediately became a uniform solution, and showed excellent solubility.

Next, the contact lens contaminated with the artificial dirt obtained in (a) was put in this lens case, left at 25 ° C. for 30 minutes to perform a washing treatment, and the result was evaluated by visual observation and absorbance at 280 nm. However, dirt was removed, and a good cleaning effect was confirmed.

For comparison, when the surface was rubbed by hand using a commercially available cleaning solution containing an abrasive, some of the convex surface could be removed to some extent, but the edge and the entire concave surface could not be removed. Further, in the case of a detergent containing no abrasive, dirt could not be removed by rubbing after soaking for 1 hour.

(C) Stability test of enzyme activity The prepared detergent was stored at 40 ° C, and after one week and one month after the detergency test of (b) was carried out, it was confirmed that sufficient detergency was maintained. I understood.

For comparison, a test was performed using a commercially available powdered enzyme. This powdered enzyme was stable in a closed state, but when dissolved in physiological saline and stored in a solution state, the cleaning activity was lost in 48 hours.

Example 2 Glycerin 40 Diethylene glycol 40 Bioprase NY 2 Magnesium ascorbic acid phosphate ester 0.1 Sorbic acid 0.1 Sorbitol 1 Distilled water Total 100 ml Evaluation was performed in the same manner as in Example 1, and the enzyme activity was reduced as compared with Example 1. However, detergency was good.

Example 3 Glycerin 50 Diethylene glycol 30 Boric acid 0.1 Ascorbic acid phosphate ester magnesium salt 0.1 Subtilisin 1 Polyethylene glycol fatty acid ester 0.2 Distilled water Total 100 ml This stain remover has good solubility and dispersibility when diluted in water. Was. In addition, the effectiveness can be expected for oil and fat stains.

Example 4 Glycerin 50 Propylene glycol 25 Boric acid 0.1 Ascorbic acid phosphoric acid ester magnesium salt 0.1 Subtilisin 2 Sorbic acid 0.1 Distilled water Total 100 ml Evaluation was performed in the same manner as in Example 1, and good detergency and enzyme activity were maintained. .

Comparative Example 1 Glycerin 20 Propylene glycol 15 Boric acid 0.1 Ascorbic acid phosphate ester magnesium salt 0.1 Subtilisin 2 Sorbic acid 0.1 Distilled water Total 100 ml When the evaluation was performed in the same manner as in Example 1, sufficient enzyme activity was shown at the beginning. After 1 month, almost no enzyme activity was detected, which was not practically sufficient.

[Effect of the Invention] The present invention utilizes an enzyme cleaning action, so that there is no residue left over when rubbing by hand, and dirt is removed by simply attaching a portion that is difficult to wash by hand. It has the effect of being able to do it. Also, because it was a water-soluble liquid,
This has the effect that the operation at the time of use is simple, the solution is easily dissolved and uniformized in the diluent, and there is no fear of deformation of the lens due to non-uniform components in the cleaning solution. Further, there is also an effect that the problem of breakage of the lens during the cleaning is eliminated because the cleaning is not performed by hand.

────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-63-159822 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) G02C 1/00-13/00

Claims (5)

(57) [Claims] 1. A dirt remover for a contact lens in the form of a solution, which comprises a protease, a polyol, a boron compound and an ascorbic acid derivative, wherein the polyol is contained in an amount of 40 to 95% by weight, and wherein the ascorbic acid derivative is contained. Of 0.01 to 1% by weight. 2. The dirt remover for contact lenses according to claim 1, wherein the pH is adjusted to 5.5 to 8.5. 3. The dirt remover for contact lenses according to claim 1, wherein said boron compound is present in an amount of 0.01% by weight or more.
A stain remover for a contact lens, characterized in that the stain remover is contained in an amount of not more than weight%. 4. The method according to claim 1, wherein said protease is present in an amount of 0.01 to 10% by weight.
A stain remover for koi tact lenses, characterized by containing. 5. The contact lens stain remover according to claim 1, further comprising Mg ions in an amount of 0.0001-0.1 mEq /.
A stain remover for contact lenses, characterized by containing.