JPH0493919A - Detergent for contact lens - Google Patents

Abstract

PURPOSE:To efficiently decompose away the stains on a contact lens surface without rubbing by the hand by intimately mixing specific enzyme and a solvent contg. a specific ratio of polyhydric alcohol. CONSTITUTION:The protease obtd. by subjecting bacillus subtilis to liquid culture is important to afford a prescribed effect as the enzyme to be used. This enzyme is exemplified by bioplaze, etc. The compsn. contg. 0.005 to 10 wt.%, more preferably 0.05 to 5 wt.% such enzyme component is preferable in order to obtain a sufficient effect. An aq. soln. contg. at least 20 wt.% of polyhydric alcohol is essential in order to maintain the stability of the enzyme activity as the soln. to dissolve or suspend such enzyme. Such polyhydric alcohol includes ethylene glycol, propylene glycol, butanediol, pentanediol, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a contact lens cleaning agent, and particularly to a novel cleaning agent that is effective in removing dirt adhering to the surface of a contact lens.

More specifically, the present invention relates to a cleaning agent that has an unprecedented proteolytic effect and is easy to use since it is in a liquid state, and is expected to rapidly develop enzyme activity.

[Conventional technology]

Conventionally, hard contact lenses whose main component is methyl methacrylate, and soft contact lenses whose main component is 2hydroxy methacrylate or N-vinylpyrrolidone have been widely used as contact lenses. However, it has been pointed out that contact lenses made of methyl methacrylate can cause complications due to insufficient oxygen supply to the cornea of the eye, and today contact lenses with higher oxygen permeability, which are safer, are becoming mainstream. . These components include organic silane compounds and fluorine-containing compounds.

However, these high oxygen permeability contact lenses tend to become contaminated when worn for a long period of time, and on the other hand, they have the disadvantage that they are more likely to break than conventional hard contact lenses, making it difficult to clean the contact lenses. Therefore, when wearing a patient's clothing, the lipids, proteins, mucin substances, and bacteria in the tear fluid,
Cosmetic contamination can result in contact lens fogging, leading to vision loss and eye damage.

Lipid stains on the surface of contact lenses can be removed using surfactants contained in general contact lens cleaning agents, but proteins and mucin cannot be easily removed, and they remain on the contact lenses and cannot be used as is. If this is continued, denaturation and coagulation will progress, resulting in even stronger adhesion, shortening the lifespan of the Funtact lens.

On the other hand, cleaning agents containing proteolytic enzymes, polysaccharide-degrading enzymes, lipolytic enzymes, or hypochlorous acid-based chemicals that have an oxidizing effect are conventionally known for cleaning contaminated contact lenses.

For example, Japanese Patent Publication No. 53-47810 discloses a cleaning method using a composition containing an effective amount of protease 7 and a cleaning agent comprising a specific proteolytic enzyme such as papain. Further, Japanese Patent Application Laid-Open No. 62-913 proposes a cleaning agent comprising a specific enzyme such as amylase or cellulase and a specific activator such as urea or an amino acid. Further, Japanese Patent Application Laid-Open No. 53-125412 discloses a cleaning agent using a lipolytic enzyme such as lipase. Further, Japanese Patent Publication No. 1-32962 discloses a composition using sodium hypochlorite or further adding a specific sugar or acid.

All of these disclosed cleaning agents can be expected to have useful effects because dirt on the surface of the contact lens can be easily removed by dipping.

[Problem to be solved by the invention]

However, among the methods described above,
No. 810, JP-A-62-913, JP-A No. 53-12541
Among the detergents described in Publication No. 2 and currently commercially available detergents containing enzymes as a main component, a solution that maintains stable enzyme activity has not been proposed.

That is, enzyme-containing detergents prepared according to the disclosed formulations almost universally lose or significantly reduce enzyme activity within 24 hours. Therefore, in order to maintain high activity, it was necessary to store it in dry tablet form. In this case, the patient is required to dissolve the solid in a special liquid each time it is used, and there is a problem that deformation occurs due to the lens being immersed in an insufficiently dissolved state.

Furthermore, in the treatment agent using chemicals disclosed in Japanese Patent Publication No. 1-32962, the chemicals themselves are highly toxic, so if sufficient rinsing is not performed, there is concern that residual treatment liquid may cause damage to the eyes.

Therefore, the present invention was achieved through intensive research and development aimed at solving these problems.

That is, an object of the present invention is to provide a solution-type contact lens cleaning agent that uses a specific enzyme with low toxicity and excellent cleaning effect, has excellent storage stability even in solution form, and is easier for patients to use. The purpose is to

[Means to solve the problem]

The present invention is based on the discovery that this object can be achieved by using a specific enzyme and a solvent composition under certain conditions.

That is, the contact lens cleaning agent of the present invention uses Bacillus subtilis.
The composition is characterized in that it is a solution-like composition in which a protease obtained by liquid culturing a proteolytic enzyme is uniformly dispersed together with a polyhydric alcohol of at least 20% by weight and 96% by weight.

The enzyme used in the present invention is Panrus subtilis (B
The proteolytic enzyme obtained by liquid culturing S. acillus 5ubtilis is important in providing the desired effect. Examples of this enzyme include biobrase (Nagase Seikagaku Kogyo).

The cleaning agent of the present invention contains 0.005 to 10% of this enzyme component.
In order to obtain a sufficient effect, it is desirable to use a composition containing 0.05 to 5% by weight, preferably 0.05 to 5% by weight.

Next, as a solution for dissolving or suspending this enzyme, an aqueous solution containing at least 20 weight 1% polyhydric alcohol is essential in order to maintain stable enzyme activity. Examples of polyhydric alcohols include dihydric alcohols such as ethylene glycol, propylene glycol, butanediol, bentanediol, hexanediol, diethylene glycol, triethylene glycol, tetraethylene glycol, and polyethylene glycol, as well as glycerin and erythritol. and polyhydric alcohols derived from sugars such as glucose, fructose, sorbitol, and xylitol. If the amount of polyhydric alcohol used is less than 20%, the addition has no effect. The appropriate amount to use is 30 to 80% by weight, but is limited by the type of alcohol used. That is, for low-molecular-weight alcohols with relatively low viscosity, 40% by weight or more is appropriate, but for ease of use, it is preferable to dilute with water to obtain an appropriate viscosity. In addition, with high viscosity alcohol, 2
Although a weight ratio of 5 to 50 weight 96 is convenient considering dilution operations during use, etc., the weight ratio is not necessarily limited to this weight ratio. The polyhydric alcohol used may be used alone or in combination, and may also include ethyl alcohol, isopropyl alcohol, acetone, and alkoxy alcohols (cellosolve), which generally mix freely with water.

In addition to these essential components, the cleaning liquid of the present invention may include some conventionally known components to obtain a desired effect. That is, in order to remove lipids, a surfactant that is not incompatible with enzymes can be included. These surfactants include sulfonates such as sodium sulfonate of polyethers, anionic surfactants such as carboxylates and phosphates, and nonionic surfactants mainly having a polyoxyethylene skeleton. Cationic surfactants such as surfactants and quaternary amine salts can be used. Also,
In addition, preservatives, chelating agents, stabilizers, pH adjusters,
It can contain neutral inorganic salts, thickeners, etc. In order to make the washing solution substantially isotonic with lachrymal fluid, it is desirable to add 0.8 to 1.0% by weight of sodium chloride.

Further, as a lubricant, dextran, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, etc. can be added.

Since the cleaning agent of the present invention is a solution-type cleaning agent obtained as described above, when using it, it is necessary to put 1 bottle in the lens case.
- 2 drops, then diluted with about 2 nl of physiological saline, distilled water, specific contact lens storage solution, etc., and immerse the lens in it for cleaning treatment. The cleaning process time was 15
The effect can be obtained in minutes, but the cleaning can be completed in 30 minutes to 2 hours.

The cleaning treatment temperature is not particularly limited, but is preferably room temperature or higher. After treatment, the contact lenses can be rinsed with distilled water or saline and then worn.

[For production]

The cleaning solution according to the present invention is composed of a specific enzyme and a solvent containing a specific amount of polyhydric alcohol, and due to the action of the enzyme, dirt on the surface of a contact lens can be efficiently decomposed and removed without having to be rubbed by hand. Furthermore, the activity of the enzyme is maintained by the action of the polyhydric alcohol, which chemically protects the enzyme.

〔Example〕

The present invention will be explained in more detail with reference to Examples below.
It is not limited to these. In addition, in the examples, parts represent parts by weight.

Example 1 As a protease, Bacillus subtilis
Proteolytic enzyme (trade name: Bioplase AP) obtained by liquid culturing of Ius 5ubt i 1 is
-L (manufactured by Nagase Seikagaku Kogyo), 10 parts of diethylene glycol, and 10 parts of distilled water were mixed, and the preservative was 0.0.
2 parts and 0.01 part of EDTA-disodium salt were added to prepare an enzyme washing solution.

The thus obtained cleaning agent was evaluated using the following criteria.

Evaluation Criteria> (a) Preparation of soiled contact lens sample Using an artificial soiling solution prepared according to the following procedure, 7Tj of protein was deposited on a contact lens.

That is, in a 200rrl beaker, 0.6 parts of albumin,
0.3 parts of globulin, 0.2 parts of egg white lysozyme, and 0.1 part of mucin were dissolved in physiological saline to make 100 mg. Next, the well-cleaned contact lens was immersed in the solution, and the soil solution was heated to 60° C. to denature the proteins and make them adhere to the surface of the contact lens.

The obtained contact lenses were dried at room temperature and used as test samples. The stain on the sample thus obtained was so firmly attached that it could not be removed even by hand rubbing.

(b) Cleaning power meter i test Commercially available contact lens storage case (capacity 3m11)
When 0.08 parts of the enzyme cleaning solution obtained in this example was added and 1.6 parts of physiological saline was added dropwise, the enzyme cleaning solution immediately became a homogeneous solution and exhibited excellent solubility.

Next, the artificial 19-dyed contact lens obtained in (a) was placed in this lens case, and the contact lens was heated at 25°C for 30 minutes.
After being left for 0 minutes, a cleaning treatment was performed, and the results were visually evaluated. As a result, stains were removed and a good cleaning effect was confirmed.

For comparison, when I scrubbed it by hand using a commercially available cleaning agent containing abrasives, I was able to remove dirt to some extent from some of the convex surfaces, but I could not remove dirt from the edges or the entire concave surface. In addition, with cleaning agents that do not contain abrasives, after 1 hour of immersion,
Even with scrubbing, the dirt could not be removed.

(c) Enzyme activity stability test The prepared cleaning agent was stored at 25℃ and 40℃, and the detergency test in (b) was conducted after 1 week, 1 month, and 3 months. It was found that the cleaning power was maintained.

For comparison, a commercially available powdered enzyme was tested. This powdered enzyme was stable in a sealed state, but when it was dissolved in physiological saline and stored in a solution state, its cleaning activity disappeared in 48 hours.

Example 2 As a protease, Bacillus subtilis
Proteolytic enzyme powder (trade name: Biobrase XL-41) obtained by liquid culturing of P. llus 5ubtilis)
6 (manufactured by Nagase Seikagaku Kogyo ■) and 10 parts of diethylene glycol, a preservative and a metal scavenger were added,
An enzyme detergent was obtained.

Evaluation was performed in the same manner as in Example 1, and good detergency and enzyme activity were maintained.

Example 3 In the composition obtained in Example 2, 0.05 parts of polyoxyethylene nonylphenol and 0.01 parts of sodium dodecylbenzenesulfonate were dissolved to prepare a cleaning liquid. This detergent had good solubility and dispersibility when diluted in water.

It can also be expected to be effective against oil and fat stains.

Implementation N4 As a protease, Bacillus subtilis (, Bacillus
5 parts of proteolytic enzyme (trade name: Bioplase NY manufactured by Nagase Seikagaku Kogyo ■) obtained by liquid culturing of Illus 5ubtilis), 5 parts of sorbitol, 8 parts of ethylene glycol, and 4 parts of distilled water were mixed, and a preservative was added. 0.
0.02 parts and 0.01 part of EDTA-2 sodium salt were added to prepare an enzyme cleaning solution.

Evaluation was performed in the same manner as in Example 1, and good detergency and enzyme activity were maintained.

Comparative Example 1 In Example 1, diethylene glycol 1 was used as the solvent.
When the detergent was dissolved with 3 parts of diethylene glycol and 17 parts of distilled water instead of using 0 parts of diethylene glycol and 10 parts of distilled water, the cleaning agent showed sufficient activity in the initial cleaning effect test, but
In the test after one week of standing, the enzyme activity was reduced to about half of the initial level, which was insufficient for practical use.

Comparative Example 2 Implementation N]. When 10 parts of ethyl alcohol and 10 parts of distilled water were used instead of 10 parts of diethylene glycol and 10 parts of distilled water as solvents, the enzyme activity was reduced to about half of the initial value in a test after being left for one week. This was insufficient for practical use.

〔Effect of the invention〕

Since the present invention utilizes enzyme cleaning action, there is no residue left behind when rubbing and printing by hand, and it also has the effect of cleaning and removing dirt from areas that are difficult to wash by hand. . In addition, since it is a water-soluble liquid, it is easy to operate during use, is easily dissolved and homogenized in a diluent, and has the effect that there is no risk of deformation of the lens due to contact with the components in the cleaning solution. Furthermore, since there is no need to scrub the lens by hand, there is an effect that the problem of damage to the lens during cleaning is eliminated.

that's all Applicant: Seiko Epson Corporation

Claims (1)

[Claims] (1) Bacillus Subtilis
tilis) obtained by liquid culture,
A contact lens cleaning agent characterized in that it is a solution composition uniformly dispersed with at least 20% by weight of polyhydric alcohol.