JP3112783B2 - How to wash, store and sterilize contact lenses - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cleaning, preserving or sterilizing contact lenses, and particularly to cleaning of contact lenses using one type of treatment liquid, regardless of whether they are non-hydrous contact lenses or hydrous contact lenses. , Storage, or sterilization.

[0002]

BACKGROUND ART Contact lenses can be broadly classified into hydrated contact lenses and non-hydrated contact lenses.
Since dirt such as proteins, lipids, and inorganic substances derived from tears and eye oils adheres, it is necessary to periodically clean these. In addition, since bacteria and the like easily adhere to a water-containing contact lens, sterilization must be performed by a method such as heat disinfection or chemical disinfection.

[0003] In order to remove protein stains adhering to the contact lens, the intended contact lens has been conventionally washed with a detergent containing a protease. Proteolytic enzymes are unstable in aqueous solutions. For example, commercially available serine proteases derived from the genus Bacillus (subtilisin A manufactured by Novo Nordisk Bioindustry Japan KK)
When stored as an aqueous solution at pH 7, 25 ° C., the enzyme activity is reduced to about 70% in 24 hours. For this reason, when a detergent containing a protease is provided as a liquid, it is necessary to incorporate an enzyme stabilizer in order to maintain the enzyme activity for a long period of time.

[0004] Specifically, JP-A-1-180515 and JP-A-2-168224 disclose a water-miscible organic liquid in a proportion of 50% by weight or more for stabilizing an enzyme. It has been proposed. In addition, Japanese Unexamined Patent Application Publication
Japanese Patent Application Laid-Open No. 93939/1992 and Japanese Patent Application Laid-Open No. 4-143718 propose that a polyhydric alcohol be contained in a proportion of 20% by weight or more. Further, Japanese Unexamined Patent Application Publication No.
No. 20 proposes a detergent containing a saccharide as an enzyme stabilizer.

However, since all the detergents contain a high concentration of an enzyme stabilizer, the osmotic pressure is higher than a physiological level, and when applied directly to a water-containing contact lens, the cleaning agent of the contact lens cannot be used. There has been a problem of causing deformation and eye irritation. Therefore, when washing or storing hydrated contact lenses with such an enzyme-containing detergent, dilute the detergent to physiological osmotic pressure using a large amount of purified water or a low osmotic pressure storage solution. We had to do it.

However, in the method of diluting the enzyme-containing cleaning agent every time the contact lens is cleaned,
In addition to the time and effort required for dilution, if the contact lens is not uniformly diluted, the treated contact lens may be deformed, or if it cannot be diluted to the correct concentration and the osmotic pressure becomes inappropriate, There are inherent problems such as causing irritation. In addition, if such a detergent is diluted to physiological osmotic pressure, enzyme stability will be extremely reduced in a short time, and the cleaning effect will be reduced. The cleaning could not be performed inside.

Further, Japanese Patent Application Laid-Open No. Sho 60-121416 proposes a method in which a contact lens is heated in an aqueous solution containing a proteolytic enzyme to simultaneously carry out washing and sterilization. However, even in this method, there is a problem that the protease is easily inactivated in the aqueous solution,
Stabilization of proteolytic enzymes is desired.

Although boric acid and borax are often used as buffering agents in enzyme-containing detergents (Japanese Patent Application Laid-Open No. 60-117417), they are used in combination with polyhydric alcohols. Is also known to be effective (JP-A-4-51015, JP-A-4-143718). However, even with this formulation, since the osmotic pressure becomes higher than the physiological level, it cannot be practically used for hydrous contact lenses.

[0009] On the other hand, on the other hand, measures have been devised to enhance the cleaning effect of the enzyme-containing cleaning agent. Inorganic salts such as calcium in the tear often form a complex with the protein, and the surface of the contact lens is degraded. It is known that by adding a metal chelating agent to the detergent, such dirt can be removed and the effect of removing proteins by enzymes can be enhanced. However, since a metal chelating agent is also known to extremely lower the stability of serine protease, conventionally, the metal chelating agent is not added to a detergent containing a protease, but is diluted by diluting the detergent. It was added to the solution and separated from the enzyme until use. For this reason, the user had to prepare two types of the enzyme-containing cleaning agent and the diluent.

[0010] It has also been proposed to use a specific metal chelating agent to mix the metal chelating agent and the proteolytic enzyme in the same liquid to reduce the influence on the stability of the enzyme. (Japanese Unexamined Patent Publication No. 4-51015) has the disadvantage that the metal chelating effect is weakened and the detergency is reduced.

[0011]

The present invention has been made in view of such circumstances, and it is an object of the present invention to stabilize proteolytic enzymes by physiological osmotic pressure and to provide an excellent washing effect. An object of the present invention is to make it possible to easily perform treatments such as cleaning, storage, and sterilization of both non-hydrous contact lenses and hydrous contact lenses using one kind of liquid cleaning agent.

[0012]

The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, stabilizing a Bacillus-derived serine protease with boric acid or borax in the presence of a metal chelating agent. It has been found that, at physiological osmotic pressure levels, the enzymatic activity is maintained for a long period of time, and the washing effect is also enhanced. Based on such findings, the present invention has been completed.

That is, the gist of the present invention is to include an effective amount of a serine protease derived from the genus Bacillus and a metal chelating agent, and further comprise boric acid and / or borax.
0.05-2% (w / v) and 0.5-% of polyhydric alcohol
By containing a 2% (w / v), such serine proteases with are stabilized at room temperature, using a treatment liquid osmotic value is adjusted to 200 to 600 mOsm / kg water, the Dispense and dilute the processing solution when necessary
The contact lens is immersed in the dispensed processing solution in a state as it is, and the contact lens is washed and stored.

Further, the present invention comprises an effective amount of a serine protease derived from the genus Bacillus and a metal chelating agent, and further contains 0.05 to 2% (w / v) of boric acid and / or borax.
0.5% to 2% of a polyhydric alcohol containing a (w / v)
As a result , the serine protease is stabilized at room temperature, and the osmotic pressure value is 200 to 600 mOs.
Using a processing solution adjusted to m / kg water, dispensing the processing solution when necessary, and leaving it as it is without dilution
Then, after immersing and cleaning the contact lens in the dispensed treatment liquid , the contact lens is washed at a temperature of 80 ° C. to 100 ° C. and sterilized. Is what you do.

[0015] Incidentally, in such present invention, advantageously, a non-ionic surfactant to further to the treatment solution and thus induced to contain in proportions of 0.001~1% (w / v).

In the present invention, the immersion washing operation of the contact lens is advantageously performed at a temperature of room temperature to 58 ° C.

[0017]

[Action and Effect] In short, in the treatment solution used in the present invention, the protease is effectively stabilized under physiological osmotic pressure and in the presence of a metal chelating agent. One-part liquefaction of a protease and a metal chelating agent is advantageously achieved. Therefore, even after long-term storage, the proteinase and metal chelating agent can exert an extremely excellent cleaning effect,
In addition, since it is adjusted to physiological osmotic pressure from the beginning, there is no need for dilution operation, just dispensing the necessary amount when necessary, regardless of whether it is non-water-containing or water-containing, all contact lenses On the other hand, it has a feature that it can be applied as it is and does not cause problems such as deformation of contact lenses and eye irritation.

By simply immersing the contact lens in the dispensed treatment solution, the contact lens can be easily and safely cleaned and stored with an excellent cleaning effect. Also, by immersing the contact lens in the dispensed treatment solution and heating the treatment solution, the contact lens can be washed and sterilized very easily.

Therefore, according to the present invention, it is possible to extremely easily carry out treatments such as washing, preservation, sterilization, etc. of a contact lens simply by dispensing and applying when necessary using one kind of treatment liquid. It becomes.

In the present invention, by further adding a predetermined amount of polyhydric alcohol to the processing solution, the enzyme stabilizing effect of the processing solution can be further enhanced, and the addition of a predetermined amount of a nonionic surfactant is further added. Thereby, the cleaning effect can be further enhanced. Further, the immersion cleaning process is performed at room temperature to 58 ° C.
By carrying out the reaction at the above temperature, a high detergency can be exhibited while effectively suppressing the inactivation of the enzyme.

[0021]

[Specific structure] Proteolytic enzymes (proteases) generally include serine proteases,
It is roughly classified into thiol protease, carboxyl protease, and metalloprotease. Also, from the origin of the enzyme,
Microorganisms, plants, and animals are classified into three types. Microorganisms are classified into bacteria, actinomycetes, filamentous fungi, and yeast. In such a classification, the treatment solution used in the present invention is used only for serine proteases produced by Bacillus among bacteria, and is contained as an essential component.

In recent years, such serine proteases have been modified by genetic manipulation, and various products are commercially available. In the present invention, it is also possible to use these serine proteases. I can do it. Specifically, "Esperase", "Subtilisin A", "Sabinase", "Durazyme" (manufactured by Novo Nordisk Bioindustry Japan KK), "Protease N" Amano "", "Protease S" Amano " (Manufactured by Amano Pharmaceutical Co., Ltd.) and "Bioprase" (manufactured by Nagase Seikagaku Corporation). The content of the enzyme cannot be unconditionally determined because the specific activities of the enzymes are different from each other, but is usually 0.0001 to 1% (w / v) so as to obtain an effective detergency.
Degree, preferably about 0.01 to 0.5% (w / v).

Further, the metal chelating agent contained as another essential component in the treatment solution used in the present invention can remove proteins such as calcium which forms a complex with proteins by inorganic salts such as calcium to remove proteins by enzymes. It is known to increase the effect while reducing the stability of serine proteases. Thus, in the present invention, such a metal chelating agent, the aforementioned Bacillus-derived serine protease,
By combining with boric acid and / or borax described below, in the presence of any metal chelating agent,
Enzyme stability could be achieved.

[0024] Specific examples of the metal chelating agent include ethylenediaminetetraacetic acid, nitrilotriacetic acid, and salts thereof.
Usually, the ratio is about 0.01 to 1% (w / v). When these metal chelators are not contained, the enzyme stability is further improved, but the cleaning effect is reduced.

Further, in the treatment liquid used in the present invention, boric acid and / or borax is used as an enzyme stabilizer and is contained as an essential component. In addition, the mechanism of enzyme stabilization is unknown, but if the concentration is too low, the enzyme stabilizing effect cannot be obtained, and if the concentration is too high, eye irritation may occur or detergency may decrease. , 0.05-2%
(W / v), preferably 0.2 to 1% (w / v).

In addition , in order to further enhance the enzyme stabilizing effect of the treatment solution, a polyhydric alcohol is further added.
You . If the concentration is too low, the enzyme stabilizing effect cannot be obtained, and if the concentration is too high, the detergency deteriorates. Therefore, the amount of addition is 0.5 to 2% (w / v). . Also,
Specifically, glycerin, diethylene glycol, polyethylene glycol, sorbitol and the like can be used.

In the present invention, the osmotic pressure of the treatment liquid needs to be adjusted to a physiological osmotic pressure, that is, 200 to 600 mOsm / kg water. The reason is that if the osmotic pressure is too low, the enzyme stability becomes poor, and if the osmotic pressure is higher than this range, the specification of the hydrous contact lens changes and further eye irritation occurs. Therefore, in order to adjust the osmotic pressure of the treatment liquid to the above range, an osmotic pressure adjusting agent will be appropriately added, but the osmotic pressure adjusting agent that can be used is not particularly limited, Various buffers described below,
If necessary, a tonicity adjusting agent such as sodium chloride, potassium chloride and glycerin can be used.

When the pH of the treatment liquid is higher than pH 8, there is an obstacle such as eye irritation, and when the pH is lower than pH 6, the enzyme activity of Bacillus serine protease decreases.
A pH of about 6 to about 8 is preferred. Therefore, in order to adjust the pH of the processing solution to this range, a buffer will be added as necessary, and boric acid and borate, as well as citric acid and organic acids such as acetic acid and their sodium, Salts such as potassium,
Further, amino acids such as glycine can be used.

Further, it is preferable to add a surfactant to the treatment liquid in order to remove lipid stains derived from eye oil and the like. As such a surfactant, a surfactant having high safety to a living body and having no influence on a contact lens is desirable, and in that respect, a nonionic surfactant is suitable. Specifically, polyoxyethylene polyoxypropylene block polymer, polyoxyethylene polyoxypropylene condensate of ethylenediamine, glycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, sucrose alkyl ester, polyoxyethylene alkylamine, polyoxyethylene Examples thereof include sorbitan fatty acid esters and polyoxyethylene hydrogenated castor oil. Among them, polyoxyethylene sorbite fatty acid esters are suitable.

In particular, in the structure shown in the following chemical formula 1,
Four of R _{1 to} R ₆ are a saturated or unsaturated fatty acid residue having 12 to 18 carbon atoms (RCO-: where R is a saturated or unsaturated aliphatic group), and the remaining two are hydrogen atoms Is a fatty acid polyoxyethylene sorbit is highly safe, has a high cleaning effect, among them,
Average number of moles of polyoxyethylene added (a + b + c + d
+ E + f) of 20 to 50 are advantageously selected because they have excellent solubility and good characteristics of not causing adsorption to a contact lens.

[0031]

Embedded image

When the amount of the surfactant added is small, the detergency becomes insufficient, whereas when the amount is too large, the detergency does not increase. Therefore, the surfactant is usually added in an amount of 0.001 to 1% (w / v). Degree.

[0033] Preservatives may be added to such a treatment solution, if necessary, for the purpose of suppressing the growth of bacteria during storage of the treatment solution, for example, sorbic acid or benzoic acid. Alternatively, salts thereof, biguanides, quaternary ammonium salts and the like are appropriately used.

Furthermore, in the treatment solution prepared in this manner, the protease has a long term activity at room temperature under physiological osmotic pressure and in the presence of a metal chelating agent. Specifically, at 25 ° C. for 6 months in 2 months
In the case of 0%, preferably, an enzyme residual activity of 70% or more is obtained. Therefore, such a treatment liquid can be dispensed when necessary while being stored for a long period of time, and applied to a non-hydrous contact lens and a hydrous contact lens without dilution. In addition, since there is no need to dilute as in the case of the conventional enzyme-containing cleaning solution, no labor is required, and problems such as deformation of the contact lens and eye irritation do not occur. If the residual enzyme activity is substantially 50% or less, the washing effect is reduced, and practical use becomes difficult.

In order to clean the contact lens using such a treatment solution, the contact lens may be immersed in the dispensed treatment solution, and the temperature condition for the immersion may be from room temperature to 58 ° C. Within the range, preferably from about 40 ° C to about 55 ° C, more preferably from about 45 ° C to about 50 ° C. If the immersion temperature is too low, the cleaning effect is not exhibited, while if it is too high, the enzyme is inactivated and the cleaning effect is not sufficiently exhibited. The immersion time is selected to be 15 minutes or more, preferably about 30 minutes to about 3 hours, and more preferably about 1 hour to about 2 hours. Further, since the treatment solution can be used as a preservation solution, washing can be performed during preservation of the contact lens. The time for immersion for storage is not particularly limited. And in order to improve the cleaning effect,
If necessary, it is also effective to rub the contact lens on the palm or puff before or after immersion.

In the case of a hydrous contact lens,
In addition to the cleaning treatment, a sterilization treatment is required, so that the contact lens is immersed in the dispensed treatment liquid and subjected to the above-described cleaning treatment, and then the treatment liquid is treated at a temperature of 80 ° C to 100 ° C, preferably. A method of sterilizing by heating to a temperature of about 100 ° C. is advantageously selected. As a result, heat sterilization can be performed after the washing, and the treatment of the hydrous contact lens can be performed extremely easily. The sterilization is performed by heating for 5 to 30 minutes, which is sufficient for sterilization, preferably for 10 to 20 minutes. In performing the above-described heat treatment, a heater whose temperature is controlled by a microcomputer or the like so as to reach a predetermined temperature can be used. And
After the sterilization treatment, the contact lens can be stored in the liquid as it is, or the contact lens can be taken out of the liquid and worn as it is, similarly to the usual handling.

[0037]

EXAMPLES Hereinafter, in order to make the present invention more apparent,
Some embodiments of the method for cleaning, storing and disinfecting contact lenses according to the present invention will be specifically described, but the present invention is not limited by the description of such embodiments. That goes without saying. Further, the present invention has the above specific configuration,
Further, it should be understood that various changes, modifications, improvements, and the like can be made based on the knowledge of those skilled in the art without departing from the spirit of the present invention, in addition to the embodiments described below. It is.

Examples 1 to 5 First, each component shown in the following Table 1 was weighed out, and purified water 100
The volume was made up to 5 ml to prepare five types of treatment solutions. Next, the pH and osmotic pressure of each processing solution were adjusted to pH,
Osmometer: Measured using HOSM-1 type. Also,
For each treatment solution, the enzyme activity was measured as follows, and after each treatment solution was stored at 25 ° C. for 70 days,
The enzyme activity was measured, and the remaining enzyme activity was calculated. And
The results are shown in Table 1.

(Measurement of enzyme activity)
To 5 ml of 6% casein solution (pH 7.0, 0.05 M aqueous solution of sodium hydrogen phosphate), add 1 ml of each treatment solution
And kept at 37 ° C. for 10 minutes.
The enzymatic reaction is stopped by adding 5 ml of a mixture of 11M trichloroacetic acid, 0.22M sodium acetate and acetic acid) and kept at 37 ° C. for a further 30 minutes. Then, 2.5 ml of 0.55 M sodium carbonate was added to 1 ml of the filtrate obtained by filtering this solution.
And 0.5 ml of a Foreign reagent were added, and the mixture was kept at 37 ° C. for 30 minutes, and then the absorption A at 660 nm was determined. Separately, 5 ml of the precipitating reagent and 5 ml of casein solution were added to 1 ml of each treatment solution, and the mixture was heated at 37 ° C. for 3 hours.
Hold for 0 minutes and add 1 ml of the filtrate obtained by filtering this solution,
After adding 2.5 ml of 0.55 M sodium carbonate and 0.5 ml of Folin reagent and maintaining the mixture at 37 ° C. for 30 minutes, 660 n
The absorption A ₀ at m was determined. The enzymatic activity for producing a non-proteinaceous substance exhibiting 660 nm absorption of 1 × 10 ⁻⁶ g of tyrosine per minute was defined as 1 unit. Enzyme activity (units / ml) = [(A- _A0 ) / As] x 50x
(11/10) (However, tyrosine at As = 660 nm 50 μg /
Enzyme residual activity (%) = (enzyme activity after storage / initial enzyme activity) × 100

[0040]

[Table 1]

COMPARATIVE EXAMPLE 1 A treatment liquid was prepared according to a recipe in which boric acid and borax in the treatment liquid prepared in Example 1 were changed to trisodium citrate dihydrate 1.0% (w / v). did. This treatment solution has an osmotic pressure:
334 mOsm / kg, pH: 7.13, enzyme activity: 112 un
its / ml. Then, this treated liquid was stored at 25 ° C. for 70 days, and the residual enzyme activity was examined. As a result, it was 42%. From a comparison between Example 1 and Comparative Example 1, it is found that the enzyme stability is increased when boric acid and borax are included.

Examples 6 and 7 and Comparative Example 2 The osmotic pressure of the processing solution was varied by changing the content of sodium chloride in the processing solution prepared in Example 5 as shown in Table 2 below. Each treatment solution was stored at 25 ° C. for 70 days, and the remaining enzyme activity was examined. The results are shown in Table 2.
A comparison between Examples 5 to 7 and Comparative Example 2 shows that the enzyme stability increases when the osmotic pressure of the treatment liquid is 200 to 600 mOsm / kg.

[0043]

[Table 2]

Comparative Examples 3 to 5 The enzymes of the treatment solution of Example 1 were used as papain W-4, respectively.
0 (manufactured by Amano Pharmaceutical Co., Ltd., plant-derived thiol protease), actinase AS (manufactured by Kaken Pharmaceutical Co., Ltd., actinobacteria-derived serine protease), and pancreatin F (manufactured by Amano Pharmaceutical Co., Ltd., animal-derived protease) Was prepared. Each treatment solution was stored at 25 ° C. for 70 days, and the remaining enzyme activity was examined. The results are shown in Table 3 below. As is clear from the comparison between Example 1 and Comparative Examples 3 to 5, only the treatment solution containing the Bacillus-derived serine protease had excellent enzyme residual activity.

[0045]

[Table 3]

Example 8 Egg white lysozyme 0.1% (w / v), sodium chloride 0.28% (w / v), disodium phosphate 1.15%
(W / v) and monosodium phosphate monohydrate 0.2
3% (w / v) was dissolved in purified water to prepare an artificial tear. Then, this artificial tear is put into a 5 ml vial, and one piece of a hydrated contact lens (manicon soft MA manufactured by Menicon Co., Ltd.) is put into the vial and heated to 80 ° C. for 20 minutes.
After cooling, it was rinsed with physiological saline to prepare a contact lens to which protein stain was artificially attached. When this contact lens was observed with a dark-field stereomicroscope, proteinaceous deposits were observed on one surface. Next, this artificially soiled lens was placed in a storage case, filled with the treatment solution prepared in Example 1, and immersed at room temperature of 25 ° C. overnight. After immersion, it was rinsed with physiological saline and observed with a dark-field stereomicroscope. As a result, it was found that dirt on the contact lens had been cleanly removed.

Example 9 The artificially soiled lens produced in Example 8 was placed in a storage case, filled with the treatment solution prepared in Example 2, and heated at 50 ° C. for 6 hours.
It was heated and washed for 0 minutes. Furthermore, after repeating this heating treatment again, it was rinsed with physiological saline and observed with a dark-field stereomicroscope. As a result, it was found that dirt on the contact lens had been cleanly removed.

Comparative Example 6 The artificially soiled lens produced in Example 8 was placed in a storage case, filled with the treatment solution prepared in Example 2, and heated at 60 ° C. for 6 hours.
It was heated and washed for 0 minutes. As a result of repeating this heat treatment twice, dirt on the contact lens was not removed so much, and dirt remained on one side. From comparison with Example 9,
In the cleaning method according to the present invention, a high cleaning power was exhibited when the temperature of the treatment liquid during the heat cleaning was 58 ° C. or lower, and it was shown that when the temperature was higher than that, the cleaning effect was reduced.

Example 10 The treatment solution prepared in Example 2 was stored at 25 ° C. for 2 months.
Then, similarly to Example 9, the artificially soiled lens was heated and washed at 50 ° C. for 60 minutes using this treatment liquid. As a result of repeating this heat treatment twice, dirt on the contact lens was completely removed.

Comparative Example 7 The treatment liquid prepared in Comparative Example 1 was stored at 25 ° C. for 2 months.
Then, similarly to Example 9, the artificially soiled lens was heated and washed at 50 ° C. for 60 minutes using this treatment liquid. As a result of repeating this heat treatment twice, dirt on the contact lens was not removed so much, and dirt remained on one side. Comparison with Example 10 shows that the cleaning method according to the present invention can provide high cleaning power even after long-term storage of the processing solution.

Example 11 The diameter of a water-containing contact lens (Menicon MA, manufactured by Menicon Co., Ltd.) was measured with a profile projector (manufactured by Nikon Corporation), and the power was measured by a lens meter (manufactured by Nikon Corporation). It was 13.0 mm and frequency -3.00D. Then, after wearing the contact lens on the human eye for 6 hours, remove it from the eyes, and rinse with a preservative solution (manufactured by Menicon Co., Ltd., clean bottle soak)
Put in storage case.

Next, 1.2 ml of the treatment solution prepared as in Example 1 was dispensed into the storage case, and the contact lens was immersed and washed for 2 hours, and then the storage case was boiled and disinfected (manufactured by Menicon Co., Ltd.). , Menicon Riser Mini), sterilized and disinfected, and then stored overnight. Then, the remaining processing solution is stored at 25 ° C.
While dispensing and using the treatment liquid, the treatment of wearing, rinsing, immersion washing, boiling and storage was repeated once a day from the next day. When the contact lens was inspected again after 60 days, there was no change in the diameter and frequency, and the contact lens surface was observed with a dark-field stereomicroscope.
It was clean.

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Claims (4)

(57) [Claims] 1. An effective amount of a serine protease derived from the genus Bacillus and a metal chelating agent, further comprising 0.05 to 2% (w / v) of boric acid and / or borax and a polyhydric alcohol.
By containing a 0.5~2% (w / v), such serine proteases with are stabilized at room temperature, osmotic value is adjusted to 200 to 600 mOsm / kg water treatment solution Dispense and dilute the treatment solution as needed
Intact in the state, cleaning of contact lenses, which comprises immersing contact lenses in the treatment solution which was dispensed該分, preservation methods without. 2. An effective amount of a serine protease derived from the genus Bacillus and a metal chelating agent, further comprising 0.05 to 2% (w / v) of boric acid and / or borax and a polyhydric alcohol.
By containing a 0.5~2% (w / v), such serine proteases with are stabilized at room temperature, osmotic value is adjusted to 200 to 600 mOsm / kg water treatment solution Dispense and dilute the treatment solution as needed
After washing by immersing the contact lens in the intact, the treatment liquid that was dispensed該分without, 80 ° C.
A method for cleaning and sterilizing contact lenses, wherein the method is performed by sterilizing by heating to about 100 ° C. Wherein the treatment liquid further, according to claim 1 or claim 2 of a nonionic surfactant characterized by containing in the proportion of 0.001~1% (w / v) Method. 4. The immersion cleaning operation of the contact lens is performed at a temperature of room temperature to 58 ° C.
Or method according to any of claims 3.