JPH08119806A - Controlling agent for red rot disease of cultured laver - Google Patents

Abstract

PURPOSE: To obtain a controlling agent for red rot disease of cultured laver, preventing laver from being impaired, improved in controlling effect, enabling short-time control, exhibiting its activity even in a diluted state equivalent to that in a concentrated state, and preventing environmental pollution, by combining a specific acid with acetic acid singly making laver itself annihilate or incapable of exterminating red rot fungi in a diluted liquid state. CONSTITUTION: This disease injury-controlling agent for cultured laver is obtained by combining an aqueous acetic acid solution with at least one kind selected from a group of adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid, fumaric acid, formic acid, lactic acid, ketoglutaric acid, phytic acid, phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, itaconic acid, propionic acid and butyric acid. The disease injuries of cultured laver, such as red rot disease can be controlled by using this agent.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a red rot fungicide for seaweed.

[0002]

2. Description of the Related Art In seaweed aquaculture, a method of acid treatment has been adopted in order to control miscellaneous algae, that is, Aonori, Ulva and Diatom. The price of seaweed will drop if aonori is mixed in the product. Various acids such as hydrochloric acid, phosphoric acid, citric acid, malic acid, phytic acid are used as these acids. Citric acid is mainly used because it is recognized as a food additive and is inexpensive.

1-2% of citric acid (pH about 2.0-2.
The processing of 5 to 10 minutes is performed in 2). In addition, the effect of exterminating red rot is also recognized, and when treated at the above-mentioned concentration, it can be exterminated within 1 to 2 minutes, so that treatment is actually carried out for 5 to 6 minutes.

A disease called red rot disease occurs rapidly when the seaweed fronds grow and can finally be harvested, and it spreads to all fishing grounds within a few days, causing the fronds to die and flow out. Therefore, the damage is great. The cause of this disease is said to be parasites of the genus Pythium or related fungi, and these microorganisms mainly grow at a temperature of around 15 ° C and are propagated by organisms or organic matter produced by the killing and decomposition of organisms. It is considered that the presence of a large amount of fronds on the sea surface satisfies the favorable growth conditions.

The red-rot fungus grows in a hyphae in the parasite,
The parasites feed and grow, and the vigorous hyphae form a large number of zoospores at the ends, resulting in a large number of zoospores in the sac. When the sac matures, it breaks and releases zoospores, which then swim in the water and attach to new parasites.
It becomes hyphae again and immediately eats parasite cells. It is normal for the damaged leaflets to be cut into pieces and fall off, which is said to be several hundred times as much damage as industrially directly eaten.

In Japanese Patent Publication No. Sho 46-35873, P-
There has been proposed a method for controlling erythema rot of red sea bream using oxybenzoic acid or its ester. This kills all bacteria, including useful bacteria, up to now with the use of antibiotics, reverse soaps, etc., but not only has a low ability to prevent mold and yeast, it is also harmless to humans. It depends on what is not.

Japanese Unexamined Patent Publication (Kokai) No. 50-12125 discloses a saturated fatty acid carboxylic acid having 1 to 4 carbon atoms, a saturated or unsaturated carboxylic acid having 2 to 4 carbon atoms, glycolic acid, lactic acid, tartaric acid, malic acid, citric acid. The algaecide containing 1 type, or 2 or more types of the organic carboxylic acid selected from is disclosed. Although this document exclusively describes the action of germicidal algae, there is no description of red-rot fungi.

Japanese Patent Publication No. 60-13647 discloses that it contains citric acid in an amount of 0.3 to 5.0% by weight and has a pH of 1.0 to 6.
Nori algae to be immersed in the treatment liquid of No. 0, extermination and prevention of disease are described.

In Japanese Patent Publication No. 60-13648, a pH of 1. is obtained by adding an inorganic acid such as hydrochloric acid, nitric acid, sulfuric acid or phosphoric acid.
A seaweed aquaculture method for controlling weeds and diseases, which is 0 to 4.0, is described.

JP-B-60-21950 discloses a fertilizer for seaweed cultivation containing phytic acid or a salt thereof as an active ingredient, a preventive effect against red rot disease, and a diatom exterminating effect.

Japanese Unexamined Patent Publication (Kokai) No. 57-8722 discloses a seaweed aquaculture method in which a seaweed net is treated with a treatment solution containing phosphoric acid or a salt thereof to remove and prevent diseases such as weeds and red rot. Has been started.

Japanese Unexamined Patent Publication (Kokai) No. 60-87202 discloses a fungicide for seawater bacteria of edible seaweed containing adipic acid as an active ingredient.

Japanese Unexamined Patent Publication (Kokai) No. 60-244245 discloses a method of algicidal treatment in which a fumaric acid in a saturated amount or more is present, and at least one of malic acid, citric acid, tartaric acid and succinic acid is used in combination. There is also a statement to the effect.

Japanese Patent Laid-Open No. 60-248121 discloses algae and acupuncture fungi by immersing in a treatment solution containing one or more of halogenated carboxylic acids such as chloroacetic acid, dichloroacetic acid and trichloroacetic acid. A seaweed aquaculture method for exterminating illness, red spot disease, etc. is disclosed.

Japanese Unexamined Patent Publication (Kokai) No. 62-21784 discloses a liquid fertilizer which is also used as a seaweed algaicide for laver cultivation, which is composed of an acidic buffer solution containing dilute hydrochloric acid as a main component, and nitrates, ammonium salts and phosphates.

Japanese Unexamined Patent Publication (Kokai) No. 62-190103 discloses a repellent for phytopathogenic fungi of the genus Pythium and Olpidiopsis, which is a causative agent of red rot and acupuncture fungus, which contains gluconic acid as an organic component.

Japanese Unexamined Patent Publication (Kokai) No. 1-279805 discloses a repellent for algae and bacteria containing monosodium fumarate and / or monopotassium fumarate as an active ingredient.

Japanese Patent Application Laid-Open No. 2-25404 discloses a pesticide containing fumaric acid and / or fumarate and at least one selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, potassium carbonate and potassium hydrogen carbonate. Disinfectants for algae and bacteria which are dissolved in water or seawater to form monosodium fumarate and / or monopotassium fumarate are disclosed.

Japanese Unexamined Patent Publication No. 2-291218 (Japanese Patent Publication No.
(JP-A-47810), there is disclosed an algae killing method in which fumaric acid in an amount exceeding the saturated amount is allowed to exist in a state of being covered with a coating means having water permeability, and an object to be treated is dipped in the treatment liquid. .

As will be described later, the extermination of algae such as blue-green algae depends exclusively on the pH of the treatment solution. Therefore, the known hydrochloric acid, phosphoric acid, phytic acid, tartaric acid, citric acid, malic acid, Whether any acid such as succinic acid or gluconic acid is used alone or in combination, it is 9 to 9 at a pH of about 2.
You can get rid of it in 10 minutes.

[0021] However, since red rot fungi have a high infectivity when they are generated, they must be quickly treated to completely kill them. However, since infection from the sea occurs immediately, the seaweed will be destroyed at intervals of 2 to 6 days unless it is treated.

Nori producers average 100 to 200 seaweed nets on average. With the current treatment, it takes a minimum of 5 minutes to process 100 seams. However, since it takes 8 hours or more, 30 to 40 sheets can be processed per day. Therefore, as the spread of red rot becomes more severe, the control of red rot fungus is delayed, and the production of seaweed ends with no production of rotten seaweed. Therefore, there is a strong demand for the development of a drug capable of controlling red rot bacteria in a shorter time.

[0023]

An object of the present invention is to provide a preparation capable of controlling red rot fungi in a short time without damaging the seaweed itself.

[0024]

[Means for Solving the Problems] As a result of intensive research to solve the above problems, an aqueous solution of acetic acid has a high damage to seaweed, and the seaweed is killed at a certain concentration or above for a certain treatment time or longer. On the other hand, below the concentration and processing time,
It has not been used at all because it has no effect against red rot fungi and cannot be used as a red rot fungicide at all. However, when this acetic acid is used in combination with adipic acid, lactic acid, phosphoric acid, etc., the bactericidal activity of the acid itself is significantly increased, and therefore it can be treated at a low concentration for a short time, and environmental pollution can also be reduced, thus completing the present invention. .

That is, the present invention is as follows. Water-soluble acetic acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid,
Fumaric acid, formic acid, lactic acid, ketoglutaric acid, phytic acid,
A red rot control agent for cultured seaweed, which comprises at least one selected from the group consisting of phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, itaconic acid, propionic acid and butyric acid.

As can be seen from Test Example 1, since acetic acid has a high degree of damage to seaweed, the seaweed is damaged and killed before the red rot fungus is exterminated. Therefore, acetic acid cannot be used alone as a control agent for red rot. However, as shown in Test Example 2 and later, it was found that by combining with other acids such as phytic acid, lactic acid and acetic acid, seaweed is not damaged and the bactericidal power of the combined acid itself can be considerably increased. It was

Currently, a muzzle boat has been developed and a short-time treatment (1
There is a fishing ground where treatment is performed within a minute), and by using acetic acid in combination with adipic acid, lactic acid, phosphoric acid, etc., it can be used at a low concentration and the amount of acid used can be reduced. In the case of use on a mobbing ship, if an inorganic acid such as hydrochloric acid or phosphoric acid is used, a concentration of 2 to 8% is required. Therefore,
By using the present invention, the amount of acid used can be considerably reduced, and the environmental pollution load on the ocean can be reduced.

The pesticide of the present invention comprises ammonium nitrate, ammonium chloride, monoammonium phosphate as a nitrogen source,
Urea, sodium nitrate, nitric acid and the like can be used in combination with 1 potassium phosphate, 1 sodium phosphate, inositol 6-phosphate phosphate and phosphoric acid as a phosphorus source. Particularly, when phosphoric acid and phosphate are used together, the damage of seaweed can be alleviated and soft seaweed can be produced.

The pesticide of the present invention is used by a method of immersing the seaweed culture net infected with red rot in the pesticide or by spraying the pesticide on the culture net. In this way, by contacting the nori leaf body infected with the disease with the pesticide,
It does not damage seaweed and selectively removes disease-causing bacteria, adherent bacteria, diatoms, etc. Treatment of aquaculture nets that do not contain disease is also carried out as a preventive measure.

[0030]

EXAMPLES The present invention will be described in more detail with reference to examples and comparative examples, but the present invention is not limited to these examples. In the following test examples,
The mark indicates that there is no disorder of laver and the red rot fungus is completely eradicated, and the symbol indicates that there is no disorder of laver and red rot is considerably suppressed, both of which are examples. is there.
Other marks are comparative examples.

Test Example 1 0.00 of acetic acid (90%)
5, 0.01, 0.05, 0.1, 0.2, 0.3W /
A W% seawater solution was prepared, and each fixed time (5 seconds, 10 seconds,
20 seconds, ... 60 seconds, 2 minutes, 3 minutes, 4 minutes, ... 10
After soaking the seaweed infected with red rot fungus, wash with seawater,
The state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot fungi and damage to seaweed. The results are shown in Table 1.
Shown in

[0032]

[Table 1]

0.005%, 0.01%, 0.0 of acetic acid
5% and 0.1% cannot be used as a controlling agent for red rot because they have no effect of controlling red rot bacteria even if they are soaked for 10 minutes, or they are damaged. From the above, acetic acid alone cannot be used as a disinfectant for red-rot fungi.

Test Example 2 0.01 W of acetic acid (90%)
/ W seawater solution, phytic acid (50%), phosphoric acid (75
%) Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, ketoglutaric acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, propionic acid, butyric acid 0.01, 0.05, 0.1, 0.2, 0.3W respectively
Nori culture infected with red rot fungus from the adjusted solution added to make it 10%, 20%, ... 60 seconds, 90 seconds,
After soaking for 2 minutes, 3 minutes, 4 minutes ... 10 minutes, the plate was washed with seawater, and the state after 2 days of static culture was observed under a microscope, and the effect of controlling red rot fungus was investigated. The results are shown in Tables 2 to 5.

[0035]

[Table 2]

[0036]

[Table 3]

[0037]

[Table 4]

[0038]

[Table 5]

(Test Example 3) 0.05 W of acetic acid (90%)
/ W% seawater solution, phytic acid (50%), phosphoric acid (7
5%), hydrochloric acid (36%), lactic acid (90%), formic acid (90
%) Is 0.01, 0.05, 0.1, 0.2,
Nori leaves infected with red rot fungus were added to the adjusted solution added to 0.3 W / W% for 10 seconds, 20 seconds, ... 60 seconds,
After dipping for 90 seconds, 2 minutes, 3 minutes, 4 minutes, ... 10 minutes, it was washed with seawater, and the state after 2 days of static culture was observed under a microscope to examine the effect of controlling red rot bacteria. Table 6 shows the results.

[0040]

[Table 6]

Test Example 4 Acetic acid (90%) 0.1 W /
Phytic acid (50%), phosphoric acid (75%) in W% seawater
%), Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, ketoglutaric acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, propionic acid, butyric acid 0.01, 0.05, 0.1, 0.2, 0.3W /
10 seconds, 20 seconds, ... 60 seconds, 90 seconds, 2 seconds of nori leaf infected with red rot fungus in the adjustment solution added to have W%
After soaking for 3 minutes, 3 minutes, 4 minutes ... 10 minutes, the plate was washed with seawater, and the state after 2 days of static culture was observed under a microscope to examine the effect of controlling red rot bacteria. The results are shown in Tables 7 to 10.

[0042]

[Table 7]

[0043]

[Table 8]

[0044]

[Table 9]

[0045]

[Table 10]

Test Example 5 Acetic acid (90%) 0.2 W /
Phytic acid (50%), phosphoric acid (75%) in W% seawater
%), Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid 0.01, 0.05, 0.1,
10 seconds, 20 seconds ... 6 seconds of nori leaf infected with red rot fungus in the adjusting agent added to 0.2, 0.3 W / W%
After soaking for 0 seconds, 90 seconds, 2 minutes, 3 minutes, 4 minutes, and 5 minutes, it was washed with seawater and the state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot bacteria. The results are shown in Table 11.

[0047]

[Table 11]

Test Example 6 Acetic acid (90%) 0.3 W /
Phytic acid (50%), phosphoric acid (75%) in W% seawater
%), Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, ketoglutaric acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, propionic acid, butyric acid, 0.01, 0.05, 0.1, 0.2, 0.3W respectively
10 seconds, 20 seconds ... 60 seconds, 90 seconds
After soaking for 20 seconds, the plate was washed with seawater, and the state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot bacteria. The results are shown in Tables 12 to 15.

[0049]

[Table 12]

[0050]

[Table 13]

[0051]

[Table 14]

[0052]

[Table 15]

(Test Example 7) (Comparative Example) Phytic acid (50%), phosphoric acid (75%), hydrochloric acid (36%)
%), Lactic acid (90%) formic acid (90%), tartaric acid, sulfuric acid,
Nitric acid, maleic acid, malic acid, citric acid, gluconic acid,
Fumaric acid, ketoglutaric acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, itaconic acid, propionic acid, butyric acid 0.01, 0.05, 0.
A 1, 0.2, 0.3 W / W% seawater solution was prepared, and the seaweed leaf bodies infected with red rot fungus were immersed for a certain period of time, then washed with seawater, and the state after 2 days of static culture was examined under a microscope. Observe at
The effect of controlling red rot fungus was investigated. The results are shown in Table 16 to Table 1.
9 shows.

[0054]

[Table 16]

[0055]

[Table 17]

[0056]

[Table 18]

[0057]

[Table 19]

Test Example 8 Various acids used in combination with acetic acid in the present invention 0.01%, 0.05%, 0.1
%, 0.2%, 0.3%, 0% acetic acid concentration,
Tables 20 to 23 show the results of measuring the red rot disinfection time when the content was changed to 0.01%, 0.05%, 0.1%, and 0.3%. It is clear that the disinfection time of each type of acid is significantly shortened as compared with the case where acetic acid is not present.

[0059]

[Table 20]

[0060]

[Table 21]

[0061]

[Table 22]

[0062]

[Table 23]

(Test Example 8) Leaves infected with red rot fungus were added to a seawater solution of the following combination obtained by adding two or more kinds of acids to acetic acid (90%) for 5 seconds, 10 seconds, 20 seconds ... 60 seconds. , 90
Second, 120 seconds immersion treatment, then wash with seawater and static culture 2
The condition after a day was observed under a microscope to investigate the effect of controlling red rot fungus. The combination formulations are shown in Table 24 below. Table 25 shows the control effect of the red rot fungus.

[0064]

[Table 24]

[0065]

[Table 25]

[0066]

[Effects of the Invention] When used alone, the degree of damage to seaweed is high,
By using acetic acid, which cannot be used for controlling red rot bacteria, together with other acids such as adipic acid, succinic acid, lactic acid, phytic acid, and phosphoric acid, the seaweed is not damaged and the bactericidal power of the acid itself is greatly increased. It was possible to get rid of red-rot fungi in time.
Since the amount of acid used can be significantly reduced, environmental pollution to the ocean can be greatly reduced.

Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI Technical display area A01N 37:36) (A01N 37/02 37:06) (A01N 37/02 37:42) (A01N 37/02 57:12) (A01N 37/02 59:26) (A01N 37/02 59:00)

Claims (2)

[Claims] 1. An aqueous solution of acetic acid is added to adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid,
Citric acid, gluconic acid, fumaric acid, formic acid, lactic acid, ketoglutaric acid, phytic acid, phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid,
An agent for controlling red rot of cultured seaweed, which comprises at least one selected from the group consisting of nitric acid, maleic acid, malic acid, itaconic acid, propionic acid, and butyric acid. 2. An acetic acid aqueous solution (including part or all of seawater) having a concentration of 0.3 W / W% or less, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid, At least one acid selected from the group consisting of fumaric acid, formic acid, lactic acid, ketoglutaric acid, phytic acid, phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, itaconic acid, propionic acid, butyric acid, The concentration in the aqueous solution is set in the range of 0.01 to 0.3 W / W% corresponding to the required treatment time, and the cultured nori is immersed in this mixed solution or the solution is sprayed on the cultured nori. A method for controlling diseases of cultured seaweed.