JPH0788287B2 - Plant growth promoter - Google Patents
Plant growth promoterInfo
- Publication number
- JPH0788287B2 JPH0788287B2 JP62329886A JP32988687A JPH0788287B2 JP H0788287 B2 JPH0788287 B2 JP H0788287B2 JP 62329886 A JP62329886 A JP 62329886A JP 32988687 A JP32988687 A JP 32988687A JP H0788287 B2 JPH0788287 B2 JP H0788287B2
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- plant growth
- ppm
- present
- growth promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、植物に対し優れた生育促進作用を示す組成物
に関し、更に詳述すれば、アミノ酸発酵液、すなわち培
養液を植物に散布することによって、植物の生育を促進
させる促進剤に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of use] The present invention relates to a composition having an excellent growth-promoting action on plants. The present invention thus relates to a promoter for promoting the growth of plants.
従来、植物に対する生育促進剤として、オーキシン、ジ
ベレリン、サイトカイニン等の他に、各種アミノ酸が知
られている。また、特公昭46−42566号公報の如く、ウ
ラシルおよびシトシンの少くとも一種とプロリンを併有
する花芽形成促進剤が開示され、この併用によって花芽
形成促進効果、着果率が向上するというものである。Conventionally, various amino acids other than auxin, gibberellin, cytokinin, etc. have been known as growth promoters for plants. Further, as disclosed in JP-B-46-42566, a flower bud formation promoter having at least one of uracil and cytosine and proline is disclosed, and the combined use improves flower bud formation promoting effect and fruit setting rate. .
特公昭56−32961号公報には、アミノ酸類のうち特定の
ものを柑橘類に投与して、果実の品質を改良する方法が
提案されている。更には特開昭61−270289号公報には、
葉面散布用肥料としてアルコール発酵、アミノ酸発酵、
核酸発酵、有機酸発酵等の発酵液から目的とする生産物
質を蒸留法、イオン交換樹脂法により回収した後の残液
を鉱酸で処理し、次いで中和した後、固形物を分離した
上澄液又は濾液にアミノ酸、核酸及び糖の少なくとも１
種を添加したものが開示されている。Japanese Examined Patent Publication No. 56-32961 proposes a method for improving the quality of fruits by administering specific ones of amino acids to citrus fruits. Furthermore, in JP-A-61-270289,
Alcohol fermentation, amino acid fermentation, as fertilizer for foliar application
After recovering the target product from the fermentation broth of nucleic acid fermentation, organic acid fermentation, etc. by distillation method, ion exchange resin method, the residual liquid is treated with mineral acid, then neutralized, and then the solid matter is separated. At least one amino acid, nucleic acid and sugar in the supernatant or filtrate
The addition of seeds is disclosed.
〔発明が解決しようとする問題点〕 しかし、植物に対する生育促進剤としてのオーキシン、
ベジレリン等各種アミノ酸単独では余り効果がないか、
効果的に一定とならない等種々の欠点を有する。また特
公昭46−42566号公報にあっては、ウラシルとプロリン
を併用することが記載されているが、イネ、キウリなど
の花芽を形成するための促進剤であって、生育促進剤で
はないこと、及びウラシルとプロリンの２種のものに限
られているものであって、本発明とは、目的は勿論のこ
と、特定の培養液である点で大きく異なる。特公昭56−
32961号公報は、柑橘類の果実の品質改良方法であり、
植物生育促進剤ではない。更に特開昭61−270289号公報
は、発酵残液を鉱酸で処理した後、中和し、上澄液又は
濾液にアミノ酸等を添加するもので、アミノ酸発酵液そ
のものを使用する本発明とは相違し、また植物の生育に
は必ずしも充分な効果が得られない欠点を有している。[Problems to be Solved by the Invention] However, auxin as a growth promoter for plants,
Whether various amino acids such as bezirelin alone have little effect,
It has various drawbacks such as not being effectively constant. In Japanese Patent Publication No. 46-42566, it is described that uracil and proline are used in combination, but it is a promoter for forming flower buds of rice, cucumber and the like, not a growth promoter. , And uracil and proline, and the present invention is greatly different from the present invention in that it is a specific culture solution as well as the object. Japanese Examined Sho 56-
No. 32961 is a method for improving the quality of citrus fruits,
It is not a plant growth promoter. Further, JP-A-61-270289 discloses that the fermentation residual liquid is treated with a mineral acid, then neutralized, and amino acids and the like are added to the supernatant or the filtrate. Are different from each other, and have the drawback that sufficient effects cannot be obtained on plant growth.
本発明者らは、これらの欠点を解決すべく鋭意研究を重
ねた結果、アミノ酸単独ではなくして、アミノ酸発酵原
料に、アミノ酸発酵用菌を使用して、出来たアミノ酸発
酵液を植物に散布することによって、植物の生育が極め
て促進されることを見い出し、本発明を完成するに至っ
た。即ち、 本発明はグルコース、塩安、ビタミンを主成分とするア
ミノ酸発酵原料にアミノ酸発酵用菌を培養させて得たア
ミノ酸発酵液からなる植物生育促進剤である。As a result of intensive studies to solve these drawbacks, the present inventors sprayed the resulting amino acid fermentation broth on the plant by using the amino acid fermentation bacterium as the amino acid fermentation raw material instead of the amino acid alone. As a result, they found that the growth of plants was extremely promoted, and completed the present invention. That is, the present invention is a plant growth promoter comprising an amino acid fermentation broth obtained by culturing an amino acid fermentation bacterium in an amino acid fermentation raw material containing glucose, ammonium chloride and vitamins as main components.
以下本発明について詳細に説明する。The present invention will be described in detail below.
本発明に使用するアミノ酸発酵原料は、グルコース、塩
安、ビタミンを主成分とし、その他の原料としては、各
種の無機物及び有機物を含有させることも可能である。
アミノ酸発酵液中のグルコースの濃度は１重量％ないし
50重量％が好ましく、さらに好ましくは５重量％ないし
20重量％である。塩安の濃度は0.5重量％ないし20重量
％が好ましく、さらに好ましくは１重量％ないし10重量
％である。また、ビタミンの添加量は0.01ppmないし10p
pmが好ましい。The amino acid fermentation raw material used in the present invention contains glucose, ammonium chloride and vitamins as main components, and as other raw materials, various inorganic substances and organic substances can be contained.
The concentration of glucose in the amino acid fermentation broth is 1% by weight to
50% by weight is preferred, more preferably 5% by weight or
20% by weight. The concentration of ammonium salt is preferably 0.5% by weight to 20% by weight, more preferably 1% by weight to 10% by weight. Also, the amount of vitamins added is 0.01ppm to 10p
pm is preferred.
本発明に使用する菌は、コリネバクテリウム、バチルス
等のアミノ酸発酵用の菌である。また本発明に言うアミ
ノ酸発酵液中のアミノ酸は、アミノ酸単独のもの及び各
種アミノ酸からなるアミノ酸混合物などの特定の組成か
らなる培養液である。これら培養液からなる植物生育促
進剤は、それ自体単独で植物に散布してもよく、またそ
れ以外のたとえば肥料、担体などとの混合物として、あ
るいは適宜の希釈剤、増量剤、懸濁化物、乳化剤などを
用いて液剤として使用してもよい。The bacterium used in the present invention is a bacterium for amino acid fermentation such as Corynebacterium and Bacillus. The amino acid in the amino acid fermentation broth referred to in the present invention is a culture broth having a specific composition such as an amino acid alone or an amino acid mixture comprising various amino acids. The plant growth promoter comprising these culture solutions may be sprayed to the plant by itself, or as a mixture with other such as fertilizer and carrier, or an appropriate diluent, extender, suspension, You may use it as a liquid agent using an emulsifier etc.
本発明の植物生育促進剤の使用方法は、一般には液状、
粉末状として散布するのが有利であるが、その他本発明
の目的が達成されるかぎり適宜な方法が用いられる。The method for using the plant growth promoter of the present invention is generally liquid,
Although it is advantageous to apply in the form of powder, any other suitable method can be used as long as the object of the present invention can be achieved.
その植物の施用方法としては、たとえば地上部でも、ま
た地下部に与えてもよい。また使用濃度は、適用植物の
種類、その使用方法等に応じて適宜定められるが、液肥
でアミノ酸濃度２〜150ppmが好ましく５〜100ppmが更に
好ましく、最適には10〜50ppmである。またアミノ酸発
酵原料としてグルコース、塩安、ビタミンを主成分とし
た理由は、グルコースについては、植物の熟した果実中
に多く存在し、また葉、茎、根、花などのすべてにも存
在する糖で、極めて植物との親和性が強く、この親和性
の強い糖から培養されたアミノ酸発酵液は植物の生育に
強く作用するものである。従って、アミノ酸を得るため
の糖類としては種々あるが、本発明の主成分たる糖とし
ては、グルコースを必須とする必要がある。また、塩安
は培地のpH調整と養分及び肥料としての役割を果す。更
にビタミンとしては、ビオチン（ビタミンＨ）、チアミ
ン（ビタミンB1）等が例示できるが、菌増殖促進剤とし
て、またビタミン欠乏菌に加えることにより、脱炭酸能
の低下を回復する等の役目をすることから必ず必要であ
る。As a method of applying the plant, it may be applied above the ground or below the ground. The use concentration is appropriately determined depending on the type of the applied plant, the method of use, etc., but the amino acid concentration in liquid fertilizer is preferably 2 to 150 ppm, more preferably 5 to 100 ppm, and most preferably 10 to 50 ppm. The reason for using glucose, ammonium chloride, and vitamins as the main ingredients for amino acid fermentation is that glucose is present in many ripe fruits of plants, and is also present in leaves, stems, roots, flowers, etc. Thus, the amino acid fermented broth, which has an extremely strong affinity with plants and is cultivated from sugars with a strong affinity, strongly affects the growth of plants. Therefore, although there are various sugars for obtaining amino acids, glucose is essential as a sugar as a main component of the present invention. In addition, ammonium chloride plays a role of adjusting pH of the medium, nutrients and fertilizer. Further, examples of vitamins include biotin (vitamin H) and thiamine (vitamin B1), but they serve as bacterial growth promoters, and when added to vitamin-deficient bacteria, they serve to restore the decrease in decarboxylation ability. It is absolutely necessary.
実施例１ 本発明に係る植物生育促進剤を得るため、グルコース、
塩安、ビタミンを主原料として、この主原料にコリネバ
クテリウム属の菌株コリネバクテリウム グルタミカム
（Corynebacterium Glutamicum）（ATCC21159）を使用
して、アミノ酸発酵を行った。すなわち無菌条件下で、
250mlの発酵フラスコ内に於いて、 グルコース 200g/ NH4Cl 50g/ ビオチン（ビタミンＨ） 0.0001g/ 尿 素 10.0g/ KH2PO4 1.0g/ MgSO4・7H2O 0.5g/ CaCO3 50g/ FeSO4・7H2O 0.01g/ MnSO4・5H2O 0.01g/ ZnSO4・7H2O 0.01g/ 塩化チアミン 0.0005g/ なる組成を持つ混合物、pH7.0で滅菌した発酵培地の10m
lにコリネバクテリウム属の菌株を接種した。このフラ
スコを振盪下30℃の温度で120時間培養する。この結
果、得られた培養液中のアミノ酸組成を調査したとこ
ろ、プロリン20g/、アラニン10g/、バリン6g/、
グルタミン酸4g/のアミノ酸の混合体が得られた。こ
の混合体から培養液を３回分取した。これらを培養液A,
B及びＣとする。これらの培養液を使用したときの、キ
ウリの生育及び果実に対する影響について調べた。すな
わち、この試験法としては、砂質土壌を詰めた1/2000ア
ールポットに播種後約30日のキウリ苗（品種、北進２
号）を１本植え、播種後40日目から14日毎に本発明の植
物生育促進剤を水に希釈して各種アミノ酸の合計濃度を
50ppmになるように調製した液を植物全体に噴霧した。
一方本発明の植物生育促進剤の代りに、本発明の植物生
育促進剤の培養液中に含まれるアミノ酸のみの水溶液、
すなわち、下記のアミノ酸液Ａ〜Ｄの４種類を作製し
た。Example 1 To obtain a plant growth promoter according to the present invention, glucose,
Amino acid fermentation was carried out using Corynebacterium glutamicum (ATCC21159), which is a strain of the genus Corynebacterium. That is, under sterile conditions,
In fermentation flask 250 ml, glucose 200g / NH 4 Cl 50g / Biotin (Vitamin H) 0.0001 g / Urea 10.0g / KH 2 PO 4 1.0g / MgSO 4 · 7H 2 O 0.5g / CaCO 3 50g / FeSO 4・ 7H 2 O 0.01g / MnSO 4・ 5H 2 O 0.01g / ZnSO 4・ 7H 2 O 0.01g / thiamine chloride 0.0005g / Mixture with composition of 10m of fermentation medium sterilized at pH 7.0
l was inoculated with a strain of Corynebacterium. The flask is incubated under shaking at a temperature of 30 ° C. for 120 hours. As a result, when the amino acid composition in the obtained culture solution was investigated, proline 20 g /, alanine 10 g /, valine 6 g /,
A mixture of glutamic acid 4 g / amino acid was obtained. The culture solution was collected from this mixture three times. These are culture medium A,
B and C. The effects of using these cultures on the growth and fruit of cucumber were investigated. In other words, this test method uses a cucumber seedling (cultivar, Hokushin 2) about 30 days after sowing in a 1/2000 are pot filled with sandy soil.
No.), and every 14 days from the 40th day after sowing, the plant growth promoter of the present invention is diluted in water to adjust the total concentration of various amino acids.
A liquid prepared to have a concentration of 50 ppm was sprayed on the whole plant.
On the other hand, instead of the plant growth promoter of the present invention, an aqueous solution containing only amino acids contained in the culture solution of the plant growth promoter of the present invention,
That is, the following four kinds of amino acid solutions A to D were prepared.
アミノ酸液A:プロリン20g/ アミノ酸液B:プロリン20g/＋アラニン10g/ アミノ酸液C:プロリン20g/＋グルタミン酸4g/ アミノ酸液D:プロリン20g/＋アラニン10g/＋バリン
6g/＋グルタミン酸4g/ これらを各々培養液と同じ希釈率（800倍）で希釈し用
いた。更に本発明の植物生育促進剤の代りに水を用い、
これを無処理液とし、相対比較をするため無処理液を10
0として比較した結果を下記第１表に示す。Amino Acid Liquid A: Proline 20g / Amino Acid Liquid B: Proline 20g / + Alanine 10g / Amino Acid Liquid C: Proline 20g / + Glutamic Acid 4g / Amino Acid Liquid D: Proline 20g / + Alanine 10g / + Valine
6 g / + glutamic acid 4 g / These were diluted with the same dilution ratio (800 times) as the culture medium and used. Further, water is used instead of the plant growth promoter of the present invention,
This is used as the untreated solution, and the untreated solution is used for relative comparison.
The results of comparison with 0 are shown in Table 1 below.
上記第１表より明らかなように、水（無処理液）を100
としたときに、アミノ酸単独又は２種以上のアミノ酸の
場合（アミノ酸液Ａ〜Ｄ）には、無処理液より多少の効
果は見られるものの大きな効果は期待出来ないことが判
明した。 As is clear from Table 1 above, 100% water (untreated liquid)
It was found that, when the amino acids were used alone or in the case of two or more kinds of amino acids (amino acid solutions A to D), some effects could be expected but no significant effects could be expected compared to the untreated solutions.
これに対して本発明である培養液は、アミノ酸液Ａ、
Ｂ、Ｃ、Ｄと比較して、茎葉重、果実重、根重をとって
みても顕著に生育していることが判明した。On the other hand, the culture solution of the present invention is the amino acid solution A,
Compared with B, C and D, it was found that the growth was remarkable even when the foliage weight, fruit weight and root weight were taken.
実施例２ 実施例１と同様にして本発明に係る生育促進剤のアミノ
酸合計濃度がキウリにどのように影響するかを３連制で
ポット試験をした。すなわち、各試験としては、播種後
40日目から14日毎に本発明に係る植物生育促進剤を水に
希釈して、アミノ酸の合計濃度を1ppm、2ppm、5ppm、10
ppm、20ppm、50ppm、100ppm、150ppmとした液を植物全
体に噴霧し、一方本発明に係る植物生育促進剤の代りに
水（無処理液）を用い、これを相対比較に於いて100と
した。その結果を下記第２表に示す。Example 2 In the same manner as in Example 1, a pot test was conducted in triplicate to determine how the total amino acid concentration of the growth promoting agent according to the present invention affects cucumber. In other words, each test was conducted after seeding
Every 14 days from the 40th day, the plant growth promoter according to the present invention is diluted in water to give a total concentration of amino acids of 1 ppm, 2 ppm, 5 ppm, 10
A solution of ppm, 20 ppm, 50 ppm, 100 ppm, 150 ppm was sprayed on the whole plant, while water (untreated solution) was used in place of the plant growth promoter according to the present invention, which was set to 100 in the relative comparison. . The results are shown in Table 2 below.
上記第２表より明らかなように、水噴霧100に対して本
発明に係る植物生育促進剤の濃度を増して行く程、生育
状態は良くなり、100ppmまでは、引続いて成育状態は向
上しているが150ppmを限度として、それ以上濃度を増加
しても効果は認められなかった。また1ppmでは効果は認
められず、少なくとも2ppmは必要である。この結果から
も好ましい濃度としては、2ppm〜150ppm、更に好ましく
は５〜100ppm、更には最適範囲として10ppm〜50ppmであ
る。 As is clear from Table 2 above, the higher the concentration of the plant growth promoter according to the present invention with respect to the water spray 100, the better the growth state, and up to 100 ppm, the growth state continues to improve. However, the effect was not recognized even if the concentration was increased beyond 150 ppm. No effect is observed at 1 ppm, and at least 2 ppm is necessary. From these results, the preferable concentration is 2 ppm to 150 ppm, more preferably 5 to 100 ppm, and further, the optimum range is 10 ppm to 50 ppm.
以上、実施例より明らかなように、本発明の植物生育促
進剤は、単なるアミノ酸水溶液による植物生育状態に比
較して、極めて優れた促進効果を有するものである。As described above, as is clear from the examples, the plant growth promoter of the present invention has an extremely excellent promoting effect as compared with a plant growth state by a simple amino acid aqueous solution.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 水島 潔 北海道砂川市日の出町15番地 (56)参考文献 特開 昭55−104991（ＪＰ，Ａ) 特開 昭61−67491（ＪＰ，Ａ) ─────────────────────────────────────────────────── --- Continuation of the front page (72) Inventor Kiyoshi Mizushima 15 No. 15 Hinodemachi, Sunagawa, Hokkaido (56) References JP-A-55-104991 (JP, A) JP-A-61-67491 (JP, A)
Claims (2)
るアミノ酸発酵原料にアミノ酸発酵用菌を培養させて得
たアミノ酸発酵液そのものを含むことを特徴とする植物
生育促進剤。1. A plant growth promoter comprising an amino acid fermentation liquid itself obtained by culturing an amino acid fermentation bacterium in an amino acid fermentation raw material containing glucose, ammonium chloride and vitamins as main components.
る特許請求の範囲第１項記載の植物生育促進剤。2. The plant growth promoter according to claim 1, wherein the amino acid fermentation liquor has a concentration of 2 to 150 ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62329886A JPH0788287B2 (en) | 1987-12-28 | 1987-12-28 | Plant growth promoter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62329886A JPH0788287B2 (en) | 1987-12-28 | 1987-12-28 | Plant growth promoter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01172310A JPH01172310A (en) | 1989-07-07 |
| JPH0788287B2 true JPH0788287B2 (en) | 1995-09-27 |
Family
ID=18226351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62329886A Expired - Lifetime JPH0788287B2 (en) | 1987-12-28 | 1987-12-28 | Plant growth promoter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0788287B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ530803A (en) * | 2001-08-02 | 2005-12-23 | Ajinomoto Kk | Fruit- or berry-quality improving agent |
| AU2006304159B2 (en) | 2005-10-14 | 2012-09-06 | Archer-Daniels-Midland Company | Fertilizer compositions and methods of using |
| CN103980047B (en) * | 2014-05-19 | 2016-05-04 | 中国水稻研究所 | Microbial manure of a kind of water resistant rice sprouts damping-off and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55104991A (en) * | 1979-02-08 | 1980-08-11 | Kikkoman Shoyu Co Ltd | Vegetable clutivation |
| JPS6167491A (en) * | 1984-09-07 | 1986-04-07 | Ajinomoto Co Inc | Preparation of plant-growth controlling component by microorganism |
-
1987
- 1987-12-28 JP JP62329886A patent/JPH0788287B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01172310A (en) | 1989-07-07 |
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