JPH0776744B2 - Spatially developed sample pattern measuring device - Google Patents

Description

Detailed Description of the Invention a. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an image acquisition device such as a two-dimensional electrophoretic gel or a thin plate for the purpose of analyzing biological fluids.

B. 2. Description of the Related Art In the two-dimensional electrophoresis gluing or thin layer plate in the above-mentioned analysis, the spots of the sample components are distributed like a star on one side of a 15 cm square and the number is more than 1000. Become. In the past, the entire surface of the gel or thin layer was scanned in order to obtain quantitative information from the spots of the sample components secondarily developed in this way. For this entire surface scanning, an image element such as a zigzag scanning method or a one-dimensional photodiode array for the entire surface using a thin light beam is used to form an image of a gel surface or the like on this image element and the image element is electrically scanned A method of performing such an operation on the entire gel surface has been used. The former method is very time consuming because it scans a wide surface of 15 cm square with a fine light beam without gaps. The latter method divides the gel surface into appropriate widths and scans each division, so the time required for measurement is shorter than the former method, but when the entire width of the sample surface is reduced and projected onto the image element, ,
Since the image resolution is reduced and the illumination illuminance is reduced, the quantitative accuracy is reduced, and therefore reduction projection cannot be performed and the width on the sample that the image element can cover at one time is smaller than the entire sample surface.
After all, it takes a considerable time to measure the entire surface of the sample.

C. DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention The present invention is intended to minimize the time loss when performing quantitative measurement on a large number of sample component spots distributed over a wide area.

D. Measures to solve problems 1000 spots formed on gel by two-dimensional electrophoresis.
Although the number is more than one, the number of spots that actually need to be quantified is often ten to several tens. It can be said that conventionally, time is wasted by uniformly performing quantitative measurement for the majority of spots that do not require quantification. The present invention provides a light source device that irradiates a certain range around a certain position on the sample stage surface, a sample stage that can be moved by a designated amount in the x direction and the y direction on the sample stage surface, and the above light source device. Corresponding to the coordinate value based on the image element where the image of the sample is formed by the reflected or transmitted light from the sample surface and the x and y coordinate data input to the sample stage memory Only x, y
A control means for driving in each direction is provided, and data of an arbitrary measurement range set according to the coordinates of a spot to be quantitatively measured on a two-dimensional electrophoretic gel or a thin layer plate and the size of the spot is displayed. This is a two-dimensional sample measuring device which is stored in a memory, and only the spot to be quantitatively measured is sequentially positioned at the light irradiation position through the control means by the data of the stored measuring range.

E. Effect According to the present invention, not a uniform measurement is performed on the entire wide sample surface, but only the necessary portions are sequentially measured.
Not only in the case of electrically scanning using the image element as described in the previous section, but also in the method of scanning only the designated measurement point with a thin light beam, there is no time for performing unnecessary measurement. Overall measurement time is reduced.

F. Embodiment FIG. Shows an embodiment of the present invention. s is the sample stage x, y
Is a pulse motor for moving the sample stage s in the x and y directions, and D1 is a drive circuit for these pulse motors. L is a light source device that focuses light on a line segment C parallel to the x-axis on the sample stage. Reference numeral 1 controls the drive circuit D1 by a computer, counts the drive pulses of the motors X and Y, and measures the sample stage S at the center point O of the light irradiation range C.
The x and y coordinate values at the coordinates fixed to are detected. Two
Is an electrophoretic gel or a thin layer plate of the sample placed on the sample stage S. P is a one-dimensional image element such as a CCD line sensor, and an image of the light irradiation area C on the sample 2 is formed on the light receiving surface of the image element P by the lens G.

The operator sets the sample 2 on the sample stage S, lights the light source device L, operates the xy movement key 3, and moves the sample stage so that the center of the spot to be quantified comes to the center O of the light irradiation area C. Is moved, the measurement range is set so that the spots to be measured are exactly included, and the read key 5 is pressed.
The coordinate values of and the data of the measurement range centered on the coordinates are stored in the memory M. Perform the same input operation one after another for the spots to be quantified, and then give a start command to the computer 1, the computer 1 reads the coordinate data and the measurement range data for each spot stored in the memory. Drive the sample stage S,
The image element P is scanned and the video signal read from the element is stored in a predetermined area of the memory M.

G. Effect According to the present invention, the image scanning is performed only on a small number of small areas on the electrophoretic gel or the thin layer plate. Shortened. Also, the area to be quantified is set to the required range according to the size of one spot, so the measurement signal of another nearby spot should be stored in the memory without being mixed with the collected data. The amount of signal data is limited to the required amount, and the memory capacity can be saved.

In the above embodiment, the spot image is projected on the image element,
Although it is electrically scanned, the present invention can also be applied to a method of scanning the sample surface with a light beam, and even at that time, the effect of shortening the measurement time due to the limited scanning range is the same.

The present invention can be applied even when the sample components are developed one-dimensionally, in which case the measurement is performed only at the spots required and the other portions may be skipped.

[Brief description of drawings]

 The drawings are block diagrams showing an embodiment of the present invention.

 ─────────────────────────────────────────────────── ─── Continuation of front page (56) References JP-A-51-23795 (JP, A) JP-A-49-130293 (JP, A) JP-A-57-57258 (JP, A) Practical application Sho-57- 118348 (JP, U)

Claims (1)

[Claims] 1. A light source device for irradiating a fixed range around a fixed position on the sample stage surface, a means for detecting the position coordinates of the sample stage, and a light receiving element for receiving the reflected or transmitted light from the sample surface. A memory for storing the data of an arbitrary measurement range set according to the coordinates of the spot to be quantitatively measured on the layer on which the sample on the sample stage is developed and the size of the spot, and the measurement range stored in this memory A spatially developed sample pattern measuring device comprising a control means for collecting measurement data from the light receiving element based on the data of 1.