JPH07508165A - 乳酸ldh遺伝子を発現する酵母菌株,及びその菌株を得るために使用可能なベクター - Google Patents
乳酸ldh遺伝子を発現する酵母菌株,及びその菌株を得るために使用可能なベクターInfo
- Publication number
- JPH07508165A JPH07508165A JP6502095A JP50209594A JPH07508165A JP H07508165 A JPH07508165 A JP H07508165A JP 6502095 A JP6502095 A JP 6502095A JP 50209594 A JP50209594 A JP 50209594A JP H07508165 A JPH07508165 A JP H07508165A
- Authority
- JP
- Japan
- Prior art keywords
- yeast
- lactic acid
- ldh
- strain
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G1/00—Preparation of wine or sparkling wine
- C12G1/005—Methods or means to load or unload, to weigh or to sample the vintage; Replenishing; Separation of the liquids from the solids before or after fermentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/024—Preparation of other alcoholic beverages by fermentation of fruits other than botanical genus Vitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/025—Low-alcohol beverages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (6)
- 1.酵母中で乳酸菌LDHをコードする遺伝子の発現を調節する配列の制御下で 、乳酸菌LDHをコードする遺伝子の少なくとも1つのコピーを含む酵母菌株。
- 2.酵母がサッカロミセス属に属する請求項1に記載の菌株。
- 3.発現される遺伝子がカゼイ菌LDHのものである請求項1又は2に記載の菌 株。
- 4.酵母中で活性を示す調節配列と組み合わされた乳酸菌LDHの遺伝子からな る発現カセット。
- 5.請求項4に記載の発現カセットからなり、かつ請求項1に記載の形質転換酵 母を得るのに使用可能な発現ベクター。
- 6.さらに、細菌の複製起点と細菌中で選択可能なマーカーを有するシャトルベ クターである請求項5に記載のベクター。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR92/07632 | 1992-06-23 | ||
FR9207632A FR2692591B1 (fr) | 1992-06-23 | 1992-06-23 | Souches de levure exprimant le gene de la ldh lactique, et vecteurs utilisables pour l'obtention desdites souches. |
PCT/FR1993/000618 WO1994000554A1 (fr) | 1992-06-23 | 1993-06-22 | Souches de levure exprimant le gene de la ldh lactique, et vecteurs utilisables pour l'obtention desdites souches |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH07508165A true JPH07508165A (ja) | 1995-09-14 |
JP3555950B2 JP3555950B2 (ja) | 2004-08-18 |
Family
ID=9431055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50209594A Expired - Lifetime JP3555950B2 (ja) | 1992-06-23 | 1993-06-22 | 乳酸ldh遺伝子を発現する酵母菌株,及びその菌株を得るために使用可能なベクター |
Country Status (11)
Country | Link |
---|---|
US (1) | US5712152A (ja) |
EP (1) | EP0651784B1 (ja) |
JP (1) | JP3555950B2 (ja) |
AT (1) | ATE216424T1 (ja) |
CA (1) | CA2137824C (ja) |
DE (1) | DE69331828T2 (ja) |
DK (1) | DK0651784T3 (ja) |
ES (1) | ES2174848T3 (ja) |
FR (1) | FR2692591B1 (ja) |
PT (1) | PT651784E (ja) |
WO (1) | WO1994000554A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006006271A (ja) * | 2004-06-29 | 2006-01-12 | Toyota Central Res & Dev Lab Inc | 乳酸生産酵母および乳酸生産方法 |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1294728B1 (it) | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
US6229046B1 (en) * | 1997-10-14 | 2001-05-08 | Cargill, Incorported | Lactic acid processing methods arrangements and products |
US6475759B1 (en) | 1997-10-14 | 2002-11-05 | Cargill, Inc. | Low PH lactic acid fermentation |
ATE400181T1 (de) * | 1998-05-13 | 2008-07-15 | Novozymes Inc | Verfahren zur verwendung von cellobiosedehydrogenase beim backen |
US6358705B1 (en) | 1998-07-16 | 2002-03-19 | Novo Nordisk A/S | Method of making proteins in transformed yeast cells |
BR0010806B1 (pt) * | 1999-05-21 | 2014-02-18 | Métodos e materiais para a síntese de produtos orgânicos | |
US7153555B2 (en) * | 2000-02-15 | 2006-12-26 | Travel Tags, Inc. | Plastic objects including lenticular lens sheets |
WO2004104202A1 (ja) * | 2003-05-22 | 2004-12-02 | Kabushiki Kaisha Toyota Chuo Kenkyusho | D-乳酸脱水素酵素活性を有するタンパク質をコードするdna及びその利用 |
FR2887258B1 (fr) * | 2005-06-17 | 2007-09-21 | Agronomique Inst Nat Rech | Souches de levures saccharomyces transformees presentant une production d'ethanol reduite par fermentation |
EP1929009A2 (en) | 2005-09-22 | 2008-06-11 | Tate & Lyle Ingredients Americas, Inc. | Improved strains for the production of organic acids |
WO2007043253A1 (ja) * | 2005-10-14 | 2007-04-19 | Toray Industries, Inc. | 酵母及びl-乳酸の製造方法 |
GB2589203B (en) * | 2018-03-05 | 2023-06-07 | Danstar Ferment Ag | Expression of heterologous enzymes in yeast for flavoured alcoholic beverage production |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4472502A (en) * | 1982-08-16 | 1984-09-18 | The Regents Of The University Of California | Malolactic gene |
JP2850033B2 (ja) * | 1990-02-28 | 1999-01-27 | 明治乳業株式会社 | 新規なl―乳酸脱水素酵素及びそれをコードする遺伝子 |
-
1992
- 1992-06-23 FR FR9207632A patent/FR2692591B1/fr not_active Expired - Fee Related
-
1993
- 1993-06-22 ES ES93913171T patent/ES2174848T3/es not_active Expired - Lifetime
- 1993-06-22 US US08/338,509 patent/US5712152A/en not_active Expired - Lifetime
- 1993-06-22 CA CA002137824A patent/CA2137824C/en not_active Expired - Lifetime
- 1993-06-22 DE DE69331828T patent/DE69331828T2/de not_active Expired - Lifetime
- 1993-06-22 EP EP93913171A patent/EP0651784B1/fr not_active Expired - Lifetime
- 1993-06-22 AT AT93913171T patent/ATE216424T1/de active
- 1993-06-22 WO PCT/FR1993/000618 patent/WO1994000554A1/fr active IP Right Grant
- 1993-06-22 JP JP50209594A patent/JP3555950B2/ja not_active Expired - Lifetime
- 1993-06-22 DK DK93913171T patent/DK0651784T3/da active
- 1993-06-22 PT PT93913171T patent/PT651784E/pt unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006006271A (ja) * | 2004-06-29 | 2006-01-12 | Toyota Central Res & Dev Lab Inc | 乳酸生産酵母および乳酸生産方法 |
Also Published As
Publication number | Publication date |
---|---|
DE69331828T2 (de) | 2002-12-12 |
ATE216424T1 (de) | 2002-05-15 |
DK0651784T3 (da) | 2002-08-26 |
FR2692591A1 (fr) | 1993-12-24 |
FR2692591B1 (fr) | 1995-06-09 |
CA2137824C (en) | 2004-03-30 |
DE69331828D1 (de) | 2002-05-23 |
PT651784E (pt) | 2002-09-30 |
US5712152A (en) | 1998-01-27 |
WO1994000554A1 (fr) | 1994-01-06 |
ES2174848T3 (es) | 2002-11-16 |
EP0651784B1 (fr) | 2002-04-17 |
EP0651784A1 (fr) | 1995-05-10 |
JP3555950B2 (ja) | 2004-08-18 |
CA2137824A1 (en) | 1994-01-06 |
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