JPH07507134A - 電気泳動方法および電気泳動最適化方法およびそれらの適用のための装置 - Google Patents
電気泳動方法および電気泳動最適化方法およびそれらの適用のための装置Info
- Publication number
- JPH07507134A JPH07507134A JP5514596A JP51459693A JPH07507134A JP H07507134 A JPH07507134 A JP H07507134A JP 5514596 A JP5514596 A JP 5514596A JP 51459693 A JP51459693 A JP 51459693A JP H07507134 A JPH07507134 A JP H07507134A
- Authority
- JP
- Japan
- Prior art keywords
- electric field
- chamber
- electrophoresis
- liquid
- alternating electric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 56
- 238000001962 electrophoresis Methods 0.000 title claims description 35
- 238000005457 optimization Methods 0.000 title claims description 5
- 230000005684 electric field Effects 0.000 claims description 52
- 239000007788 liquid Substances 0.000 claims description 35
- 239000000126 substance Substances 0.000 claims description 22
- 238000000926 separation method Methods 0.000 claims description 21
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 10
- 239000002245 particle Substances 0.000 description 9
- 239000010408 film Substances 0.000 description 8
- 238000009826 distribution Methods 0.000 description 7
- 239000000470 constituent Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000037230 mobility Effects 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 239000003792 electrolyte Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000005192 partition Methods 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- -1 polyethylene terephthalate Polymers 0.000 description 4
- 239000004642 Polyimide Substances 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000005520 electrodynamics Effects 0.000 description 3
- 238000005370 electroosmosis Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920000052 poly(p-xylylene) Polymers 0.000 description 3
- 229920002492 poly(sulfone) Polymers 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 229920001721 polyimide Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 229910052814 silicon oxide Inorganic materials 0.000 description 3
- 239000003990 capacitor Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001676573 Minium Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000220010 Rhode Species 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 239000003989 dielectric material Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910003437 indium oxide Inorganic materials 0.000 description 1
- PJXISJQVUVHSOJ-UHFFFAOYSA-N indium(iii) oxide Chemical compound [O-2].[O-2].[O-2].[In+3].[In+3] PJXISJQVUVHSOJ-UHFFFAOYSA-N 0.000 description 1
- 229910052809 inorganic oxide Inorganic materials 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003094 perturbing effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- KKEYFWRCBNTPAC-UHFFFAOYSA-L terephthalate(2-) Chemical compound [O-]C(=O)C1=CC=C(C([O-])=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-L 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- XOLBLPGZBRYERU-UHFFFAOYSA-N tin dioxide Chemical compound O=[Sn]=O XOLBLPGZBRYERU-UHFFFAOYSA-N 0.000 description 1
- 229910001887 tin oxide Inorganic materials 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Electrostatic Separation (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9202396A FR2687931A1 (fr) | 1992-02-28 | 1992-02-28 | Procedes d'electrophorese et d'optimisation d'electrophorese et appareil pour leur mise en óoeuvre. |
| FR92/02396 | 1992-02-28 | ||
| PCT/FR1993/000182 WO1993016789A1 (fr) | 1992-02-28 | 1993-02-24 | Procedes d'electrophorese et d'optimisation d'electrophorese et appareil pour leur mise en ×uvre |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07507134A true JPH07507134A (ja) | 1995-08-03 |
Family
ID=9427189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5514596A Pending JPH07507134A (ja) | 1992-02-28 | 1993-02-24 | 電気泳動方法および電気泳動最適化方法およびそれらの適用のための装置 |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0627955A1 (enrdf_load_stackoverflow) |
| JP (1) | JPH07507134A (enrdf_load_stackoverflow) |
| FR (1) | FR2687931A1 (enrdf_load_stackoverflow) |
| WO (1) | WO1993016789A1 (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003008958A1 (fr) * | 2001-07-11 | 2003-01-30 | Olympus Corporation | Element d'electrophorese a flux libre et procede d'electrophorese a flux libre |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2172801T3 (es) * | 1996-07-12 | 2002-10-01 | November Ag Molekulare Medizin | Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2508844C2 (de) * | 1975-02-28 | 1984-06-14 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Einrichtung zur trägerfreien kontinuierlichen Ablenkungselektrophorese |
| US5059294A (en) * | 1985-12-26 | 1991-10-22 | The University Of Puerto Rico | Method for separating nucleic acids and nucleic acid probes |
| EP0256552A3 (en) * | 1986-08-18 | 1988-05-11 | Milan Bier | Process and apparatus for recycling isoelectric focusing and isotachophoresis |
-
1992
- 1992-02-28 FR FR9202396A patent/FR2687931A1/fr active Granted
-
1993
- 1993-02-24 WO PCT/FR1993/000182 patent/WO1993016789A1/fr not_active Application Discontinuation
- 1993-02-24 JP JP5514596A patent/JPH07507134A/ja active Pending
- 1993-02-24 EP EP93905421A patent/EP0627955A1/fr not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003008958A1 (fr) * | 2001-07-11 | 2003-01-30 | Olympus Corporation | Element d'electrophorese a flux libre et procede d'electrophorese a flux libre |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2687931B1 (enrdf_load_stackoverflow) | 1994-04-22 |
| FR2687931A1 (fr) | 1993-09-03 |
| EP0627955A1 (fr) | 1994-12-14 |
| WO1993016789A1 (fr) | 1993-09-02 |
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