JPH0739351B2 - Method to increase water intake by boosting water intake of livestock or pets - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a method for increasing the water intake of animals such as livestock and pets and increasing the water withdrawal to prevent dehydration of the animals. More specifically, the present invention relates to the addition of at least 0.5% (by weight, the same hereinafter) of a protein degradation product to drinking water or a liquid containing a drug, dissolving, dispersing, and administering to the animal to obtain water of the animal. The present invention relates to a method for breeding an animal, which enhances the willingness to ingest and increases the amount of drinking water to prevent the animal from suffering from dehydration.

[Technical background and description of prior art]

It is important to give sufficient drinking water to livestock and pets for normal breeding. Particularly in livestock, it is extremely important for normal growth or treatment of livestock to administer sufficient drinking water or a liquid containing necessary drugs (hereinafter also referred to as drinking water) during the lactation period, weaning period or illness. is there.

Generally, in domestic animals or pets, water consumption is extremely reduced and died from dehydration due to causes such as diarrhea, malnutrition or transport, or changes in the breeding environment. In this case, it is difficult to administer a drug such as an antibiotic by dissolving it in drinking water because the amount of drinking water decreases.

For example, in pig farming, it is extremely important to prevent the death of the pigs being raised in order to improve management efficiency. There are various causes of pig death, but diarrhea during the lactation period or weaning or the weakness due to malnutrition accounts for the majority, and it is said that the total number of live births reaches 10 to 15%. Therefore, utmost care should be taken to maintain the nutritional physiological condition of young piglets in good condition, and to take appropriate detention to restore the physical strength of debilitated piglets at an early stage. Administration has important implications.

Conventionally, as a countermeasure against such a case, various oral replacement liquid preparations that are dissolved in drinking water and administered to animals are commercially available, for example, Enbiger (manufactured by Nisshin Seifun Co., Ltd.), Electroplus A (manufactured by Kyoritsu Shoji Co., Ltd.). Etc. are known. These commercial products consist of minerals, glucose, glycine and dextrin. Glucose and glycine are added to these commercially available oral rehydration preparations, and therefore, by dissolving them in drinking water and administering them to animals, they have an efficiency to enhance not only nutritional aspects but also preference and increase the amount of drinking water. .

However, the animal breeding method using conventional products is
It is not a satisfactory method in terms of improving the palatability of drinking water, and a sufficient increase in drinking water is often not observed.

The present inventors have conducted repeated studies on a method for raising young piglets, and by using an aqueous solution having a high degree of preference in which protein degradation products are dissolved as supplementary water for piglets, the amount of drinking water increases, The present invention was completed based on these findings based on the finding that the weight gain rate is improved in normal piglets and that the physical strength is remarkably restored in debilitated piglets.

[Object and Summary of Invention]

It is an object of the present invention to provide a method for raising an animal (livestock, het, etc.) which has a strong willingness to ingest water, increases the amount of drinking water, prevents the animal from suffering from dehydration, and increases the weight. It is in.

Another object of the present invention is to provide an animal rearing method for a debilitated larva (livestock, pets, etc.) that enhances motivation for water intake of the larva and increases water consumption to recover physical strength. Especially.

The present invention that achieves the above object is to add at least 0.5% (by weight) of a protein degradation product to a liquid containing drinking water or a drug, dissolve, disperse, and administer to an animal (livestock, pet, etc.). This is a characteristic animal breeding method.

[Specific Description of the Invention]

Animals to which the method of the present invention is applied are domestic animals such as cattle, pigs and chickens that are bred for business, and so-called pets such as dogs and cats that are bred at home.

The protein degradation product used in the method of the present invention is produced as follows.

Acid casein, rennet casein, casein such as alkali casein, whey protein isolated from whey, soy protein such as isolated soybean, egg white, fish meat protein isolated from fish meat, microbial protein isolated from yeast, etc., or these One kind or a mixture of two or more kinds is dispersed or dissolved in water at a concentration of 5 to 15% (a salt can be added and dissolved if necessary, and the pH can be adjusted). Then, it is hydrolyzed by a conventional method. A commercially available proteolytic enzyme is added, and the protein is hydrolyzed by maintaining the optimum temperature of the enzyme. Hydrolysis is continued until the formol nitrogen content reaches at least 25%, preferably 30-45% of the total nitrogen content. After hydrolysis, heat the reaction mixture (for example, at 85 ℃ for 10 minutes) to inactivate the enzyme, add nutrients such as minerals and sugars as needed, and then concentrate and dry it by the conventional method to obtain powder. To obtain a protein degradation product of. If necessary, nutrients such as minerals, vitamins and sugars may be added in powder form to the powdery protein decomposition product.

The powdery decomposition product thus obtained is dispersed and dissolved in drinking water or the like at a concentration of at least 0.5%, preferably 1 to 5%, and administered to animals. When dispersed or dissolved in drinking water, it is freely given, and when dispersed or dissolved in a liquid containing a drug, a predetermined amount is given to each animal.

Hereinafter, the method of the present invention will be described in more detail with reference to experimental examples and examples.

Experimental Example 1 Tests were carried out on the relationship between the preference as a casein degradation product and the preference as the hormone nitrogen content of the casein degradation product.

(1) Preparation of sample 1-1) Casein decomposition product Commercially available 10% aqueous solution of sodium casein in 100% of commercially available pancreatin [4.5 times the potency titer, manufactured by Amano Co., Ltd.] and Amano A [commodity] (Manufactured by Amano Pharmaceutical Co., Ltd.) was added, and the mixture was kept at 50 ° C. for 30 hours, and then the reaction solution was heated at 85 ° C. for 10 minutes to inactivate the enzyme. The obtained casein decomposition liquid was concentrated to a solid content of 40% (by weight) by a conventional method using a vacuum concentrator, and then a powder of casein decomposition product was obtained by a spray dryer.

1-2) Casein degradation product A powder of casein degradation product was obtained in the same manner as in 1-1) except that the reaction time was 20 hours.

1-3) Casein degradation product A casein degradation product powder was obtained in the same manner as in 1-1) except that the reaction time was 14 hours.

1-4) Casein degradation product A casein degradation product powder was obtained in the same manner as in 1-1) except that the reaction time was 6 hours.

1-5) Casein divided product A powder of casein degradation product was obtained in the same manner as in 1-1) except that the reaction time was 2 hours.

The decomposition values of the components of each casein fragment were as shown in Table 1.

(2) Experimental method Drinking water containing 15% of 6-week-old healthy young piglets in each casein hydrolyzate or glucose at a concentration of 3% to 15 piglets in normal lactation, and water in a cafeteria system [supervised by Hiroshi Morimoto "Animal Nutrition Testing Method," 1st Edition, p. 186,
Published by Yokendo Co., Ltd. (1971)], they were allowed to freely ingest for 8 hours, and the intake of each drinking water was measured every 2 hours over time.

(3) Experimental result The experimental result was as shown in FIG.

In FIG. 1, the vertical axis is the intake of drinking water, and the horizontal axis is the elapsed time. A is drinking water containing a casein degradation product, and B is B.
Is drinking water containing hydrolyzed casein, C is drinking water containing hydrolyzed casein, D is drinking water containing hydrolyzed casein, E is drinking water containing hydrolyzed casein, F is drinking water containing glucose, and G is what The respective intakes of drinking water not containing

When the drinks having the highest intakes over time are arranged in the following order, there is almost no difference between the drinks containing casein degradation products and the drinks containing glucose.

Drinking water containing casein degradation products, drinking water containing casein degradation products, drinking water containing casein degradation products, drinking water containing casein degradation products, drinking water containing casein degradation products, drinking water containing glucose, none Not drinking water, glucose is often used to increase fluid replacement preference,
It can be seen that the casein decomposition products and sweeteners, which are sweeteners that have been added conventionally, have higher and higher preference than glucose.

Since the intake of drinking water containing a casein hydrolyzate having a formal nitrogen content of 25.1% is almost the same as the intake of drinking water containing glucose, in order to increase the intake than drinking water containing glucose, It is necessary to increase the amount of formol nitrogen in protein degradation products to more than 25%, but the intake of drinking water containing casein degradation products with 49.2% formol nitrogen is the casein degradation with 44.7% formol nitrogen. If the amount of formol nitrogen contained in the proteolysis product is lower than the intake of drinking water containing the product, the osmotic pressure will increase,
Since it may cause diarrhea, it is understood that it is necessary to suppress the amount of formol nitrogen in the proteolytic degradation product to about 45%.

Experimental Example 2 A test was conducted on the relationship between the concentration of the protein degradation product and the preference.

(1) Experimental method Drinking water containing 5%, 3%, 1%, 0.5% and 0.1% of the casein hydrolyzate prepared in the same manner as in 1-3) of Experimental Example 1 in the piglet of Experimental Example 1. Water and water were freely ingested by the cafeteria method of Experimental Example 1 for 8 hours, and the intake of each drinking water was measured every 2 hours over time.

(2) Experimental result The experimental result was as shown in FIG.

In FIG. 2, the vertical axis is the intake of drinking water, and the horizontal axis is the elapsed time. A is drinking water containing 3% casein degradation product, B is drinking water containing 5% casein degradation product, and C is 1. % Drinking water containing 0.5% casein hydrolyzate, D drinking water containing 0.5% casein hydrolyzate, E drinking water containing 0.1% casein hydrolyzate and F drinking water containing nothing .

In FIG. 2, the intake of drinking water containing 3% of the casein degradation product was the largest, and the intake of drinking water containing 0.1% of the casein degradation product was almost the same as the intake of drinking water containing nothing. Even when drinking water containing 5% or more of the casein degradation product was given, the intake did not increase.

In addition, other proteins were used in place of casein, and other proteolytic enzymes were used in place of pancreatin, and similar experiments were performed.In both cases, the same results as casein degradation products were obtained. Was given.

From this, it is understood that it is preferable to give drinking water containing the protein degradation product at a concentration of at least 0.5% (preferably 1 to 5%).

Experimental Example 3 Tests were carried out for protein degradation products and weight gain.

(1) Test sample 1-1) Drinking water containing a casein degradation product prepared in the same manner as in 1-3) of Experimental Example 1 at a concentration of 3%, 1-2) Glucose at a concentration of 3% Drinking water, 1-3) Drinking water containing nothing, (2) Experimental method 30 healthy three-week-old weaning piglets bred by commercial artificial milk were divided into 3 groups of 10 groups each. The test sample was given water to each group, the body weight was measured 7 days later, and the weight gain rate (%) was calculated from the following formula.

Weight gain rate (%) = [(WS) / S] × 100 W: weight after 7 days (Kg) S: weight at the start of the test (Kg) (3) Experimental results Experimental results are shown in Table 2. It was as it was.

The weight gain rate of the administration group given the drinking water containing the casein degradation product was significantly higher than that of the other two groups at a risk rate of 5%.

Experimental Example 4 A test was conducted on the relationship between administration of a casein degradation product and death of frail piglets.

(1) Test sample 1-1) Drinking water containing a casein degradation product prepared in the same manner as in 1-3) of Experimental Example 1 at a concentration of 3%, 1-2) A commercially available oral composition having the following composition 3% replacement fluid formulation
Water contained at the concentration of, (Composition of oral rehydration formulation) Sodium chloride 7.02g Sodium citrate 5.16g Sodium bicarbonate 5.04g Dipotassium hydrogen phosphate 1.38g Magnesium sulfate 0.60g Glucose 21.60g Glycine 9.00g Dextrin 10.20g (2 ) Experimental method 10 10-week-old debilitated weaned piglets bred by commercially available artificial milk were divided into 2 groups of 5 each, and each group was given a test sample and bred for 20 days, The body weight after 20 days was measured, and the rate of weight increase (%) was calculated in the same manner as in Experimental Example 3.

(3) Experimental results The experimental results are shown in Table 3.

The mortality rate of the drinking water group containing the casein degradation product was 0%, while the mortality rate of the drinking water group containing the commercially available oral rehydration formulation was 40%. Also, the body weight gain rate tended to be higher in the drinking water group containing the casein degradation product than in the drinking water group containing the commercially available oral rehydration formulation.

Experimental Example 5 Various proteolytic products were tested for their relationship of preference.

(1) Test sample and its preparation 1-1) Drinking water containing the casein degradation product of Experimental Example 1 The powder of casein degradation product prepared in the same manner as in 1-4) of Experimental Example 1 was dissolved in water, Drinking water containing casein hydrolyzate at a concentration of 3% was obtained.

1-2) Drinking water containing soybean protein hydrolyzate Powder of soybean protein hydrolyzate prepared in the same manner as in 1-3) of Experimental Example 1 except that commercially available soybean protein was used instead of commercially available sodium caseinate Then dissolve to 3%
Drinking water containing soybean protein degradation product was obtained at the concentration of.

1-3) Drinking water containing a whey protein hydrolyzate Whey protein degradation prepared in the same manner as in Experimental Example 1 1-3) except that a commercially available dairy protein was used instead of the commercially available casein sodium 3% by dissolving the powder of the product in water
Drinking water containing whey protein hydrolyzate was obtained at a concentration of.

1-4) Drinking water containing nothing The analysis values of the components of the powder of the soybean protein hydrolyzate and the powder of the whey protein hydrolyzate were as shown in Table 4.

(2) Experimental method Using the above test samples, the intake of drinking water of each test sample was measured in the same manner as in (2) of Experimental Example 1.

(3) Experimental result The experimental result was as shown in FIG.

In FIG. 3, the vertical axis is the intake of drinking water, and the horizontal axis is the elapsed time. A is drinking water containing a whey protein hydrolyzate, and B is B.
Is the drinking water containing the casein hydrolyzate, C is the drinking water containing the soy protein hydrolyzate, and D is the drinking water containing nothing.

According to FIG. 3, it can be seen that the above three types of protein degradation products all have the same preference for piglets.

Example 1 5 kg of commercially available isolated soybean protein (trade name: Fuji Pro D, manufactured by Fuji Purina) was dissolved in water 95, heat sterilized at 75 ° C for 10 minutes, and cooled to 50 ° C. To this solution, pancreatin [4.5 times the potency titer. Amano Pharmaceutical Co., Ltd.] 300 g was added, and the mixture was kept at 50 ° C. for 24 hours to hydrolyze the protein (formol nitrogen content of the decomposed product: 32.6%). Then 80
The enzyme was inactivated by heating at 0 ° C for 10 minutes, concentrated in a vacuum concentrator to a solid content of 30% by a conventional method, and spray-dried. Drinking water containing 3.0% of this soybean protein decomposition product is 6-week-old, commercially available sample of 7 underdeveloped piglets with an average weight of 5.7 kg (commercial name: Maruhi Inma lactating compound lead milk A) When administered as a replacement fluid (made by Hiwa Sangyo Co., Ltd.) and bred, the average weight was 19.3 kg after 4 weeks.
(Weight gain: 240%) He recovered to almost normal weight and no death occurred.

Example 2 10 kg of commercially available casein (sodium casein, produced in New Zealand) was dissolved in 90 hot water at 80 ° C.
It was sterilized by heating for minutes and cooled to 50 ° C. To this solution, 300 g of Pronase AS [trade name, manufactured by Kaken Kagaku Co., Ltd.] was added and kept at 50 ° C. for 12 hours to hydrolyze the protein (formal nitrogen content: 36.7%). Then, it was heated at 85 ° C. for 10 minutes to inactivate the enzyme, concentrated to a solid content of 34% using a vacuum concentrator by a conventional method, and spray-dried. Drinking water containing this casein hydrolyzate at a concentration of 1% was 12-week-old, 5 underdeveloped beagle dogs with an average weight of 3.2 kg, and commercially available dog food (trade name: One Rack Meal, Morimilk Pet Food) When raised and fed freely as a lactate, often ingested drinking water containing the casein decomposition product liquid,
Along with that, the appetite increased, and after 4 weeks, the average weight was 4.8 kg.
Then, recovery until reaching the normal range was observed.

Example 3 Commercially available whey protein (New Zealand, protein content: 7
6.5%) 10 Kg was dissolved in water 90, heat sterilized at 75 ° C for 10 minutes, and cooled to 50 ° C. Coclase SS in this solution
250 g (trade name, manufactured by Sankyo) was added, and the mixture was kept at 50 ° C. for 12 hours to hydrolyze the protein (amount of formol nitrogen: 26.3
%). Then, the mixture was heated at 80 ° C. for 10 minutes to inactivate the enzyme, concentrated by a vacuum concentrator to a solid content of 30% by a conventional method, and spray-dried. 1% of this whey protein hydrolyzate was added to a drinking water containing a commercially available oral rehydration formulation [trade name: Electroplus A, manufactured by Kyoritsu Shoji Co., Ltd.] at a concentration of 2.5%.
Was dissolved in the above-mentioned concentration, and a suitable amount of commercially available tetracycline and penicillin were dissolved, and the resulting mixture was administered to 15 calves aged 6 to 12 days with diarrhea at a rate of 2 per animal and raised.
For 2 days after the start of administration, only this solution is given 2-3 times a day,
After that, milk replacer was added as needed. As a result, the average number of treatment days required for diarrhea recovery was 4.8 days, and the death rate was 0%.
And was extremely good.

Example 4 7 kg of commercially available egg white (trade name: egg white powder, manufactured by Taiyo Kagaku) was added to water 9
It was dissolved in 3, sterilized by heating at 60 ° C for 10 minutes, and cooled to 50 ° C. To this solution, 175 g of pancreatin [4.5 times as potent as AFM, manufactured by Amano Pharmaceutical Co., Ltd.] and 170 g of Prozyme [trade name, manufactured by Amano Pharmaceutical Co., Ltd.] were added, and the mixture was kept at 47 ° C for 15 hours to hydrolyze the protein. Decomposed (formal nitrogen content: 3
0.2%). Then, the mixture was heated at 85 ° C for 10 minutes to inactivate the enzyme, the precipitated densities of the decomposition solution were filtered with a filter using Celite as a filter aid, and the resulting clear liquid was subjected to solid content using a vacuum concentrator by a conventional method. It was concentrated to a content of 30% and spray dried. An average weight of 43.5Kg of drinking water and a powdered commercial compounded sample (brand name: Maruhi Ink Pig breeding compounded lead glore, manufactured by Hiwa Sangyo) containing this egg white decomposition product at a concentration of 2.0%
When administered to 12 pigs for 4 weeks and raised, the average weight
It was 71.3 kg and good growth was observed.

〔The invention's effect〕

 The effects of the present invention are as follows.

(1) Increase water consumption of diarrhea and malnourished livestock and pets, and promote recovery of symptoms.

(2) To increase the water intake of livestock or pets in the growing season to promote their growth.

(3) The preference for drinking water containing a drug or the like is increased, the drug administration is facilitated, and the therapeutic effect is enhanced.

[Brief description of drawings]

FIG. 1 is a diagram showing the amount of drinking water added to various substances over time, FIG. 2 is a diagram showing the amount of drinking water taken up over time according to the concentration containing casein hydrolyzate, and FIG. 3 contains the degradation products of various proteins. It is a chart showing the intake amount of drinking water over time.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification number Reference number within the agency FI Technical indication location A61K 38/17 (72) Inventor Saburo Oizumi 8-7-4 Funakoshi-cho, Yokosuka City, Kanagawa Prefecture (72) Inventor Mitsunori Takase 138-10 Minami-Nakamaru, Omiya City, Saitama Prefecture (72) Inventor Yoshiyuki Tanaka 1-10-8 Nihombashi Horidomecho, Chuo-ku, Tokyo Within Ando Kasei Co., Ltd. (72) Inventor Toru Nakajima Kagoshima City, Kagoshima Prefecture Minamiei 4-chome No. 4, Hiwa Sangyo Co., Ltd. (56) Reference JP 61-1355 (JP, A) JP 59-102383 (JP, A) JP 57-186233 (JP, A) Special Kai 56-123921 (JP, A) JP 52-122400 (JP, A) JP 52-96776 (JP, A) JP 48-1483 (JP, B1)

Claims (4)

[Claims] 1. A milk protein hydrolyzate having a hormonal nitrogen content of 30 to 45% of the total nitrogen content is added to drinking water or a liquid containing a drug at a concentration of 1 to 5% (by weight). A method for raising an animal, which comprises dissolving, dispersing, and administering to livestock or a pet to increase the water intake of the animal to increase the amount of drinking water and prevent the animal from suffering from dehydration. 2. The method for raising animals according to claim 1, wherein the milk protein is selected from the group consisting of casein, whey protein and mixtures thereof. 3. A casein hydrolyzate having a formal nitrogen content of 30 to 45% relative to the total nitrogen content in drinking water is added in an amount of 1 to 5%.
% (By weight), dissolved, dispersed and administered to livestock or pets to increase the water intake of the animal and increase the amount of drinking water, thereby preventing the animal from suffering from dehydration. The animal breeding method according to claim 1, which is characterized by the above-mentioned. 4. The livestock or pet is selected from the group consisting of cattle, pigs, chickens, dogs and cats, and any one of claims 1 to 3 is set forth. Animal breeding method.