JPH072520B2 - Preservation liquid dispenser required - Google Patents
Preservation liquid dispenser requiredInfo
- Publication number
- JPH072520B2 JPH072520B2 JP61005631A JP563186A JPH072520B2 JP H072520 B2 JPH072520 B2 JP H072520B2 JP 61005631 A JP61005631 A JP 61005631A JP 563186 A JP563186 A JP 563186A JP H072520 B2 JPH072520 B2 JP H072520B2
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- tube
- pump
- temperature
- photoelectric sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は、保存温度と使用温度の異なる液体を分注する
装置に関するもので、保存から分注まで自動的に行うこ
とを可能にした装置に関するものである。TECHNICAL FIELD The present invention relates to a device for dispensing liquids having different storage temperatures and operating temperatures, and a device capable of automatically performing storage to dispensing. It is about.
特に、生物学実験の中で頻度の高い作業の一つに細胞の
培養がある。培養を行うためには、その細胞に適した培
養状態で培養しなければならない。そのため培養液をあ
る期間ごとに新しい培養液と交換する必要がある。本発
明はこのような作業を自動化する装置に関するものであ
る。In particular, one of the most frequent tasks in biological experiments is cell culture. In order to carry out culturing, the cells must be cultivated in a culturing state suitable for the cells. Therefore, it is necessary to replace the culture solution with a new culture solution every certain period. The present invention relates to a device for automating such work.
（従来の技術） 細胞の培養液の交換を行う場合、人間がピペットを用い
て培養容器内の古い液体を吸引して、そして４℃に保管
された新しい培養液を37℃に温めた後、ピペットを用い
て分注するといった作業を繰り返して行っていた。(Prior Art) When exchanging the culture solution of cells, a human being uses a pipette to aspirate the old liquid in the culture container and warm the new culture solution stored at 4 ° C to 37 ° C, The work of dispensing with a pipette was repeated.
（発明が解決しようとする問題点） 通常、培養容器のサンプル数は非常に多いため、作業者
は単純な繰返し作業を行う。それに加えて、交換時間間
隔は常に一定でないため、時間的に不規則な作業を行わ
なければならなかった。(Problems to be Solved by the Invention) Usually, since the number of samples in the culture container is very large, the worker performs simple repetitive work. In addition to that, the replacement time interval is not always constant, so that the work has to be performed irregularly in time.
これを自動化することが本発明の解決しようとする問題
点である。Automating this is a problem to be solved by the present invention.
（問題点を解決するための手段） 第１図に示すような要保存液体分注装置の構成にした。
同図において１はT1℃の保温庫,2は液体保存瓶,3は保存
液体,4は液体を検知する第１の光電センサー,5は液体の
温度を使用時温度T2℃にするための保温庫,6はポンプ,7
は液体を検知する第２の光電センサー,8は温度センサ
ー,9は液体が通るチューブ,10はピペットマニピュレー
ター,11は洗浄液槽,12は培養容器,13は廃液槽である。
各機能については下記の「作用」の項で説明する。(Means for Solving Problems) The liquid preserving device requiring preservation as shown in FIG. 1 is configured.
In the figure, 1 is a T 1 ℃ heat storage box, 2 is a liquid storage bottle, 3 is a storage liquid, 4 is a first photoelectric sensor for detecting the liquid, and 5 is the temperature of the liquid for use at T 2 ℃. Warm box, 6 is pump, 7
Is a second photoelectric sensor for detecting a liquid, 8 is a temperature sensor, 9 is a tube through which the liquid passes, 10 is a pipette manipulator, 11 is a washing liquid tank, 12 is a culture container, and 13 is a waste liquid tank.
Each function will be described in the "Operation" section below.
（作用） 液体を分注する際、T1℃の保温庫１中の保存瓶２で保存
された液体３を第２の光電センサー７が検知するまでポ
ンプ６を作動させ、次に保温庫５でチューブ９内の液体
の温度をT2℃にする。温度センサー８でチューブ内の液
体がＴ ℃になったことを確認して後、培養容器12に吐
出する。(Operation) When dispensing the liquid, the pump 6 is operated until the second photoelectric sensor 7 detects the liquid 3 stored in the storage bottle 2 in the T 1 ° C warming chamber 1 and then the warming chamber 5 The temperature of the liquid in the tube 9 is brought to T 2 ° C. After confirming that the temperature of the liquid in the tube has reached T ° C. with the temperature sensor 8, the liquid is discharged into the culture container 12.
培養液を吐出した後、チューブ９内に残った液体を再使
用するためにポンプを逆に動作させ、保存瓶２に戻す。
その後チューブ内を洗浄しておくため、ピペットマニピ
ュレーター10を動かして、洗浄液槽11より洗浄液を吸引
し、T1℃保温庫１内の第１の光電センサー４で洗浄液を
検知するまでポンプを作動させ、その後そのチュブ内の
洗浄液を廃液槽13に捨てる。After discharging the culture solution, the pump is operated in reverse to reuse the liquid remaining in the tube 9 and returns it to the storage bottle 2.
After that, in order to wash the inside of the tube, the pipette manipulator 10 is moved to suck the washing liquid from the washing liquid tank 11, and the pump is operated until the first photoelectric sensor 4 in the T 1 ° C warming chamber 1 detects the washing liquid. After that, the cleaning liquid in the tube is discarded in the waste liquid tank 13.
（実施例） 第２図は本発明の実施例であって、個々の制御および全
体の動作管理は制御装置40が行う。(Embodiment) FIG. 2 shows an embodiment of the present invention, in which the control device 40 performs individual control and overall operation management.
保存液体23は保存瓶22に入れてT1℃で保温された保温庫
21内に保存されている。この液体を培養トレイ32に分注
する際、まず制御装置40がポンプ26へ動作指令を与え
る。この液体を第２の光電センサー27が検知すれば、そ
の信号を受けて制御装置40はポンプの動作を停止する。
その後、ヒーター41で保温庫25内の温度をT2℃に設定す
る。チューブ29内の液体が設定温度T2℃になったことを
温度センサー28で検知すればピペットマニピュレーター
30で吐出口を廃液槽32の真上部に位置しておいて、再び
ポンプを動作させ、光電センサー34でチューブ吐出口ま
で液体が充填されたことを検知すれば、ポンプの駆動を
停止する。次にピペットマニピュレーター30を動作させ
て、培養トレイ32の分注容器の真上に吐出口先端を移動
させ、必要分注量に応じてポンプを作動させ液体を分注
する。Preservation liquid 23 is put in preservation bottle 22 and kept warm at T 1 ℃
Stored in 21. When dispensing this liquid to the culture tray 32, the control device 40 first gives an operation command to the pump 26. When the second photoelectric sensor 27 detects this liquid, the control device 40 receives the signal and stops the operation of the pump.
After that, the temperature inside the heat storage box 25 is set to T 2 ° C by the heater 41. If the temperature sensor 28 detects that the temperature of the liquid in the tube 29 has reached the set temperature T 2 ° C, the pipette manipulator
When the discharge port is located directly above the waste liquid tank 32 at 30 and the pump is operated again and it is detected that the liquid has been filled up to the tube discharge port by the photoelectric sensor 34, the drive of the pump is stopped. Next, the pipette manipulator 30 is operated to move the tip of the discharge port directly above the dispensing container of the culture tray 32, and the pump is operated according to the required dispensing amount to dispense the liquid.
液体分注終了後ピペットマニピュレーターのシリンジ先
を洗浄するため、ピペットマニピュレーターを移動させ
て洗浄液槽から洗浄液を吸引し、次にピペットマニピュ
レーターを移動させてチューブ内の洗浄液を全て廃液槽
に捨てる。After the end of the liquid dispensing, in order to wash the syringe tip of the pipette manipulator, the pipette manipulator is moved to suck the washing liquid from the washing liquid tank, and then the pipette manipulator is moved to discard all the washing liquid in the tube to the waste liquid tank.
チューブ内の洗浄液が全て吐出されたことは、光電セン
サー34で吐出先からの落下液体の未検知が一定時間続い
た状態で判断する。チューブ内の洗浄は上記の洗浄液吸
引吐出動作を繰り返すことによって行う。The fact that all the cleaning liquid in the tube has been discharged is determined by the photoelectric sensor 34 in a state in which the liquid drop from the discharge destination has not been detected for a certain period of time. The inside of the tube is washed by repeating the above-mentioned washing liquid suction / discharge operation.
（発明の効果） 本発明の要保存液体分注装置によれば、実験作業者が単
純な作業を繰り返す必要なく、特に細胞培養などの生物
学実験における培養液の交換作業を自動化することが可
能となる。(Effects of the Invention) According to the liquid preservation dispensing apparatus of the present invention, it is possible for the experimenter to automate the exchange operation of the culture solution in a biological experiment such as cell culture without the need for the experimenter to repeat simple operations. Becomes
第１図は本発明の要保存液体分注装置の構成図，第２図
は本発明の実施例説明図である。 1,21……保温庫、2,22……保存瓶、3,23……保存液体、
4,24……第１の光電センサー、5,25……保温庫、6,26…
…ポンプ、7,27……第２の光電センサー、8,28……温度
センサー、9,29……チューブ、10,30……ピペットマニ
ピュレーター、11,31……洗浄液槽、12……培養容器、3
2……培養トレイ、13,33……廃液槽、34……光電センサ
ー、40……制御装置、41……ヒーター。FIG. 1 is a block diagram of a liquid dispensing device requiring preservation of the present invention, and FIG. 2 is an explanatory diagram of an embodiment of the present invention. 1,21 …… Insulation box, 2,22 …… Storage bottle, 3,23 …… Storage liquid,
4,24 …… First photoelectric sensor, 5,25 …… Insulation box, 6,26…
… Pump, 7,27 …… Second photoelectric sensor, 8,28 …… Temperature sensor, 9,29 …… Tube, 10,30 …… Pipette manipulator, 11,31 …… Washing liquid tank, 12 …… Culture container , 3
2 …… Culturing tray, 13,33 …… Waste liquid tank, 34 …… Photoelectric sensor, 40 …… Control device, 41 …… Heater.
Claims (1)
先端から前記液体を分注し洗浄液を吸引し吐出するチュ
ーブと、該チューブの一部が内部を通過しチューブ内の
前記液体の温度をT2℃にし、該温度を検知する温度セン
サーを有する保温部と、前記液体を前記保存瓶から該保
温部の前記チューブに移送する液体移送路と、前記液体
移送路の端部および前記チューブの根元端に接続され、
前記液体を前記液体移送路を経て前記チューブの先端よ
り分注させ、前記洗浄液を吸引・吐出させるポンプと、
該ポンプの作動を制御する制御装置とを備え、前記液体
移送路端には該保存瓶の出口付近に前記液体を検知する
第１の光電センサーが、前記チューブには前記保温部の
出口付近に前記液体を検知する第２の光電センサーが取
り付けられ、前記制御装置は、使用直前に前記ポンプを
作動させて前記保存瓶中の前記液体を前記チューブに前
記第２の光電センサーで検知するまで移送し、前記温度
センサーで前記液体の温度がT2℃になったことを確認後
前記ポンプを作動させて前記チューブ先端から前記液体
を分注し、分注後前記チューブ内に残った液体を前記保
存瓶に戻すために前記ポンプを作動し、前記洗浄液を前
記第１の光電センサーで検知するまで吸引し、検知後該
洗浄液を吐出することを特徴とする要保存液体分注装
置。1. A storage bottle for storing a liquid at a temperature T 1 ° C.,
A tube that dispenses the liquid from the tip and sucks and discharges the cleaning liquid, and a temperature sensor that detects the temperature by making the temperature of the liquid inside the tube T 2 ° C by passing a part of the tube Section, a liquid transfer path for transferring the liquid from the storage bottle to the tube of the heat retaining section, connected to an end of the liquid transfer path and a root end of the tube,
A pump that dispenses the liquid from the tip of the tube via the liquid transfer path and sucks and discharges the cleaning liquid,
A first photoelectric sensor for detecting the liquid near the outlet of the storage bottle at the end of the liquid transfer path, and a control device for controlling the operation of the pump, and near the outlet of the heat retaining unit for the tube. A second photoelectric sensor for detecting the liquid is attached, and the controller transfers the liquid in the storage bottle to the tube until it is detected by the second photoelectric sensor by operating the pump immediately before use. Then, after confirming that the temperature of the liquid has reached T 2 ° C with the temperature sensor, the pump is operated to dispense the liquid from the tube tip, and after dispensing, the liquid remaining in the tube is A preserving liquid dispensing device characterized in that the pump is operated to return to the storage bottle, the cleaning liquid is sucked until it is detected by the first photoelectric sensor, and the cleaning liquid is discharged after the detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61005631A JPH072520B2 (en) | 1986-01-13 | 1986-01-13 | Preservation liquid dispenser required |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61005631A JPH072520B2 (en) | 1986-01-13 | 1986-01-13 | Preservation liquid dispenser required |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62168900A JPS62168900A (en) | 1987-07-25 |
| JPH072520B2 true JPH072520B2 (en) | 1995-01-18 |
Family
ID=11616498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61005631A Expired - Lifetime JPH072520B2 (en) | 1986-01-13 | 1986-01-13 | Preservation liquid dispenser required |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH072520B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63184743A (en) * | 1986-09-16 | 1988-07-30 | Konica Corp | Direct positive type silver halide photographic sensitive material |
| JP2015000106A (en) * | 2013-06-13 | 2015-01-05 | 日立アロカメディカル株式会社 | Dispensing device |
| CN105705571B (en) | 2013-10-30 | 2018-04-10 | 日本聚乙烯株式会社 | Conductive polyethylene resin combination and use its formed products and layered product |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5473094A (en) * | 1977-11-21 | 1979-06-12 | Olympus Optical Co Ltd | Automatic chemical analytical apparatus |
| JPS5631650A (en) * | 1979-08-24 | 1981-03-31 | Hitachi Ltd | Partial injection unit of solution |
-
1986
- 1986-01-13 JP JP61005631A patent/JPH072520B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62168900A (en) | 1987-07-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |