JPH0693821B2 - Additives for fish feed - Google Patents
Additives for fish feedInfo
- Publication number
- JPH0693821B2 JPH0693821B2 JP61016739A JP1673986A JPH0693821B2 JP H0693821 B2 JPH0693821 B2 JP H0693821B2 JP 61016739 A JP61016739 A JP 61016739A JP 1673986 A JP1673986 A JP 1673986A JP H0693821 B2 JPH0693821 B2 JP H0693821B2
- Authority
- JP
- Japan
- Prior art keywords
- ascorbic acid
- fish
- feed
- salmon
- additive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Furan Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 本発明は養殖魚類に対してアスコルビン酸活性を有し、
かつ飼料中で安定な、特に経時的に安定なアスコルビン
酸誘導体を含有する養魚飼料用添加物および該添加物を
添加してなる養魚飼料を提供する。TECHNICAL FIELD The present invention has ascorbic acid activity on cultured fish,
The present invention also provides an additive for fish feeds, which contains an ascorbic acid derivative that is stable in feeds, particularly stable over time, and a fish feed that is obtained by adding the additive.
従来の技術 Ｌ−アスコルビン酸（ビタミンＣ）は養殖魚類において
欠乏または不足すると壊血病症状を呈し死に至る等の重
大な被害が発生することが知られている。例えば、1962
年に各地のニジマス養魚場で脊椎のわん曲を主徴とする
異常魚が多発したが研究の結果アスコルビン酸の不足に
よることが証明された（日本水産学会第31巻第818頁〜8
26頁）。さらに昭和42年日本水産学会年会でニジマス、
ヒメマスおよびシロザケ稚魚のアスコルビン酸欠乏によ
る変形症の報告がある。また、アユでは食欲不振、軽度
の眼球突出、ヒレ基部の出血、えらぶた、下顎部の損傷
などの欠乏症、ハマチ稚魚では接餌量減少、成長停止、
脊椎わん曲、体色異変、高へい死率などの欠乏症、ウナ
ギでは食欲低下、成長停滞のほかヒレ、頭部の出血など
がおこる。さらにまた、ニジマス、ヒメマス、シロザ
ケ、ギンザケ、アユ、アマゴ、ヤマメ、ハマチ、タイ、
コイ、ウナギなどの養殖に供される魚類は飼育中のスト
レスなどで天然魚に比較してアスコルビン酸要求量が高
く飼料中のアスコルビン酸が不可欠である。2. Description of the Related Art L-ascorbic acid (vitamin C) is known to cause serious damage such as death due to scurvy symptoms when deficiency or deficiency occurs in cultured fish. For example, 1962
In 2010, abnormal fishes with spinal flexion as the main symptom occurred frequently in rainbow trout fish farms in various places, but as a result of the study, it was proved to be due to a lack of ascorbic acid (The Fisheries Society of Japan, Vol. 31, pp. 818-8).
Page 26). In addition, rainbow trout at the annual meeting of the Japanese Fisheries Society in 1942,
There is a report of deformity due to ascorbic acid deficiency in juvenile Japanese salmon and chum salmon. Also, in sweetfish, anorexia, mild exophthalmos, fin bleeding, fin lids, deficiencies such as damage to the lower jaw, decreased feeding volume and growth arrest in juvenile yellowtail,
Spinal curvature, color change, deficiency such as high death rate, decreased appetite in eels, stagnant growth, fins, and head bleeding. Furthermore, rainbow trout, hime salmon, chum salmon, coho salmon, ayu, amago, yamame trout, hamachi, Thailand,
Fish such as carp and eel, which are used for aquaculture, have higher ascorbic acid requirement than natural fish due to stress during breeding, and ascorbic acid in the feed is essential.
従って、養魚飼料にはをアスコルビン酸を含むビタミン
類が添加され、給餌されている。ところが、アスコルビ
ン酸は水溶性ビタミンの中でも特に不安定なものである
ため、飼料中に添加した場合に分解が起こる。就中、蛋
白源である魚粉中では特に不安定であり、ニジマス用飼
料のように魚粉が半ば以上を占めるような配合のもので
は分解によるビタミンＣの力価の低下の問題は非常に大
きい。Therefore, vitamins containing ascorbic acid are added to the fish feed for feeding. However, ascorbic acid is a particularly unstable water-soluble vitamin, so that it decomposes when added to feed. In particular, it is particularly unstable in fish meal, which is a protein source, and the problem of reduction in the titer of vitamin C due to decomposition is very large in a composition such as a rainbow trout feed in which fish meal occupies more than half.
即ち、Ｌ−アスコルビン酸は分解されやすく養魚飼料に
添加しても速やかに失活し、その効果を持続させること
はできない。That is, L-ascorbic acid is easily decomposed and quickly deactivates even when added to fish feed, and its effect cannot be sustained.
発明が解決しようとする問題点 上記の事情に鑑み、安定な、特に経時的に安定な、アス
コルビン酸活性を充分に有する養魚飼料用添加物が強く
求められている。本発明者らは、鋭意研究を重ねた結
果、Ｌ−アスコルビン酸−２−リン酸エステルの塩類を
養魚飼料中に添加した場合に、極めて安定、特に長期間
にわたってアスコルビン酸の力価の低下がほとんどない
ことを見出し、Ｌ−アスコルビン酸−２−リン酸エステ
ルの塩類を添加した養魚飼料で各魚種を飼育したとこ
ろ、アスコルビン酸欠乏による脊椎わん曲、眼球突出な
どの壊血病症状を防止し、へい死率を極端に低下させ、
平均体重を増加させるなどの好結果をもたらすこと見出
し本発明を完成した。Problems to be Solved by the Invention In view of the above circumstances, there is a strong demand for a stable, particularly stable with time, additive for fish feeds having sufficient ascorbic acid activity. As a result of intensive studies, the present inventors have found that when a salt of L-ascorbic acid-2-phosphate ester is added to a fish feed, it is extremely stable, and in particular, the titer of ascorbic acid decreases over a long period of time. It was found that there is almost no, and when each fish species was bred with a fish feed containing L-ascorbic acid-2-phosphate ester salts, scurvy symptoms such as spinal curvature and exophthalmos due to ascorbic acid deficiency were prevented. And significantly reduce the death rate,
The present invention has been completed, which has been found to bring about favorable results such as an increase in average body weight.
問題点を解決するための手段 本発明は飼料中で極めて安定で、かつアスコルビン酸活
性を有する養魚飼料用添加物および養魚飼料に関する。
即ち、本発明は、 １）有効成分としてＬ−アスコルビン酸−２−リン酸エ
ステルの塩類を含有することを特徴とするアスコルビン
酸活性を有する養魚飼料用添加物、 ２）養魚がニジマス、ヒメマス、シロザケ、ギンザケ、
アユ、アマゴ、ヤマメ、ハマチ、タイ、コイ、ウナギ、
フグ、ヒラメ、テラピヤ、アジ、シマアジ、マグロ、カ
ツオまたはフナである上記１）記載の養魚飼料用添加
物。および ３）有効成分としてＬ−アスコルビン酸−２−リン酸エ
ステルの塩類を蛋白源を50％以上含有する飼料に添加し
てなることを特徴とするアスコルビン酸活性を有する養
魚飼料に関する。Means for Solving the Problems The present invention relates to a fish feed additive and a fish feed that are extremely stable in feed and have ascorbic acid activity.
That is, the present invention is: 1) an additive for a fish-feeding feed having ascorbic acid activity, characterized in that it contains salts of L-ascorbic acid-2-phosphate as an active ingredient, 2) a fish-cultivating rainbow trout, hime trout, Chum salmon, coho salmon,
Ayu, Amago, Yamame, Hamachi, Thailand, Carp, Eel,
The additive for fish feed according to the above 1), which is blowfish, flounder, terra papaya, horse mackerel, striped horse mackerel, tuna, skipjack or crucian carp. And 3) a fish-feeding feed having ascorbic acid activity, which is characterized in that a salt of L-ascorbic acid-2-phosphate ester is added as an active ingredient to a feed containing 50% or more of a protein source.
以下、本発明を詳しく説明する。Hereinafter, the present invention will be described in detail.
本発明で使用されるＬ−アスコルビン酸−２−リン酸エ
ステルの塩類はＬ−アスコルビン酸−２−リン酸エステ
ルと塩を形成するものものであれば良いが、ナトリウ
ム、カリウム、カルシウム、マグネシウムが適当であ
る。The salt of L-ascorbic acid-2-phosphate ester used in the present invention may be one that forms a salt with L-ascorbic acid-2-phosphate ester, but sodium, potassium, calcium, magnesium may be used. Appropriate.
アスコルビン酸要求量は魚種や育成段階により異なる。
ニジマス、アマゴ、アユ、アマダイ、ハマチなどはアス
コルビン酸欠乏症による変形魚が発生する場合が多い。
また、ビタミン類は摂取したタンパク質を始めとする各
種栄養素の正常な代謝に必要であり、一般に代謝の盛ん
な若齡期にその要求性が高いが、アスコルビン酸は仔稚
魚期における骨格形成に必要な結晶組織コラーゲンの生
合成にとって必須である。さらに、ふ化後からアスコル
ビン酸を含む飼料を摂取するまでの間は産卵前の卵のア
スコルビン酸含有量がふ化した仔魚の正常な骨格形成因
子となるため、仔稚魚用飼料、親魚用飼料ともにアスコ
ルビン酸の重要性が強調される。Ascorbic acid requirements vary depending on the fish species and the stage of development.
In rainbow trout, amago, ayu, pearl oyster, hamachi, etc., deformed fish due to ascorbic acid deficiency often occur.
In addition, vitamins are required for normal metabolism of various nutrients such as ingested protein and are generally highly required during the juvenile stage when metabolism is active, but ascorbic acid is required for skeletal formation during the larval stage. It is essential for the biosynthesis of various crystalline collagens. In addition, since the ascorbic acid content of eggs before spawning becomes a normal skeletal morphogenetic factor for hatched larvae after hatching until ingestion of a diet containing ascorbic acid, both ascorbine feed and larval feed are ascorbate. The importance of acid is emphasized.
本発明のＬ−アスコルビン酸−２−リン酸エステルの塩
は、例えばニジマス稚魚の場合には飼料1kgあたり２ミ
リモル以上添加されればコラーゲンの代謝異常に基づく
変形魚の発生を予防することができ、負傷、細菌感染に
も充対応できるようになる。Ｌ−アスコルビン酸−２−
リン酸エステルの塩はいずれも魚体内の酵素によりＬ−
アスコルビン酸となり、アスコルビン酸活性を発揮す
る。The salt of L-ascorbic acid-2-phosphate ester of the present invention can prevent the generation of deformed fish due to abnormal metabolism of collagen by adding 2 mmol or more per 1 kg of feed in the case of rainbow trout fry, for example. It will be able to deal with injuries and bacterial infections. L-ascorbic acid-2-
All phosphate salts are L-
Becomes ascorbic acid and exerts ascorbic acid activity.
本発明において、Ｌ−アスコルビン酸−２−リン酸エス
テルの塩類の使用量、添加量は、一概には規定し難い
が、対象とする養殖魚の種類、使用目的、添加混合され
るべき飼料成分、組成その他の要因により決定される。In the present invention, the amount of the L-ascorbic acid-2-phosphate ester salt to be used and the amount to be added are difficult to determine in general, but the type of the cultured fish to be targeted, the purpose of use, the feed components to be added and mixed, Determined by composition and other factors.
本発明の飼料用添加物の対象となる養魚は養殖用の魚種
であれば良いが、ニジマス、ヒメマス、シロザケ、ギン
ザケ、アユ、アマゴ、ヤマメ、ハマチ、タイ、コイ、ウ
ナギ、フグ、ヒラメ、テラピヤ、アジ、シマアジ、マグ
ロ、カツオ、フナが適当である。The target fish culture of the feed additive of the present invention may be any fish species for aquaculture, but rainbow trout, hime salmon, chum salmon, coho salmon, ayu, amago, yamame, hamachi, thailand, carp, eel, puffer fish, flounder, Terrapia, horse mackerel, striped horse mackerel, tuna, bonito, and crucian carp are suitable.
本発明の飼料用添加物は通常使用されている飼料組成に
添加して用いることができる。例えば、魚粉などの蛋白
源、α−デンプン類、ビタミン類、その他の飼料組成で
あるが、蛋白源が50％以上含まれる飼料にＬ−アスコル
ビン酸−２−リン酸エステルの塩類を添加し飼料製剤化
することができ、飼料中でのアスコルビン酸活性は長期
間にわたって持続される。The feed additive of the present invention can be used by adding it to a feed composition which is usually used. For example, a protein source such as fish meal, α-starch, vitamins, and other feed compositions, but a feed containing 50% or more of the protein source added with L-ascorbic acid-2-phosphate ester salts It can be formulated and the ascorbic acid activity in the feed is sustained over a long period of time.
実施例および比較例 次に実施例を挙げて、本発明をさらに具体的に説明する
が、本発明はこれにより制限されるものではない。Examples and Comparative Examples Next, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.
実施例１および比較試験例１ 白魚粉55％、α−ポテトスターチ32％、大豆油５％、マ
ックコラム塩３％、アスコルビン酸を含まないビタミン
混合物５％からなるペレット飼料（基本飼料）を調製
し、これをアスコルビン酸欠乏区飼料とした。この基本
飼料成分1kgにさらにアスコルビン酸の２ミリモルを加
えて調製したものをアスコルビン酸添加区飼料、また基
本飼料成分1kgに本発明化合物中のＬ−アスコルビン酸
−２−リン酸マグネシウムの２ミリモルを加えて調製し
たものを本発明化合物添加区飼料とした。但し、ビタミ
ン混合物5gには塩酸チアミン6mg、リボフラビン20mg、
塩酸ピリドキシン4mg、ビタミンB120.009mg、ニコチン
酸80mg、塩化クロリン800mg、イノシトール400mg、パン
トテン酸カルシウム28mg、ビオチン0.6mg、葉酸1.5mg、
α−トコフェノール40mg、メマデイオン4mg、カルシフ
ェロール0.05mg、酢酸レチネン20mg、セルローズ3596mg
を含む。以上のペレット飼料をニジマスの浮上稚魚に飽
食量を給餌し、100日間飼育した。各試験区あたり200尾
を飼育し、給餌回数は給餌開始の０〜80日後が１日６
回、81〜100日後が１日４回とした。飼料は給餌開始の5
0日前に調製したものを使用した。経時的に各区のニジ
マス稚魚の平均体重を調査した。また、各区の生存尾
数、変形尾数を経時的に調査し、第１表に示した。Example 1 and Comparative Test Example 1 Pellet feed (basic feed) consisting of 55% white fish meal, 32% α-potato starch, soybean oil 5%, Maccolum salt 3%, and ascorbic acid-free vitamin mixture 5% was prepared. This was used as ascorbic acid deficient diet. 1 mg of this basic feed ingredient was further added with 2 mmol of ascorbic acid to prepare a feed containing ascorbic acid, and 1 kg of basic feed ingredient with 2 mmol of L-ascorbic acid-2-magnesium phosphate in the compound of the present invention. What was additionally prepared was used as the feed containing the compound of the present invention. However, in 5 g of the vitamin mixture, thiamine hydrochloride 6 mg, riboflavin 20 mg,
Pyridoxine hydrochloride 4 mg, vitamin B 12 0.009 mg, nicotinic acid 80 mg, chlorin chloride 800 mg, inositol 400 mg, calcium pantothenate 28 mg, biotin 0.6 mg, folic acid 1.5 mg,
α-tocophenol 40 mg, memadeion 4 mg, calciferol 0.05 mg, retinene acetate 20 mg, cellulose 3596 mg
including. Floating rainbow trout larvae were fed with the above pellet feed at a satiety amount and raised for 100 days. 200 test animals are raised in each test area, and the number of feedings is 0 to 80 days after the start of feeding 6 a day.
81 to 100 days later, four times a day. Feed starts 5
The one prepared 0 days before was used. The average weight of juvenile rainbow trout in each plot was investigated over time. In addition, the number of surviving fish and the number of modified fish in each plot were investigated over time and shown in Table 1.
その結果、平均体重に区間の差はほとんどなかった。し
かし、アスコルビン酸欠乏区と比べ、アスコルビン酸類
添加区では変形魚の出現数、死亡率が低下し、特にＬ−
アスコルビン酸−２−リン酸マグネシウムを添加した区
では変形尾数の出現を完全に抑えた。As a result, there was almost no difference in the average body weight between the sections. However, compared to the ascorbic acid deficient group, the number of deformed fish and the mortality rate decreased in the ascorbic acid-added group, and especially L-
In the group to which ascorbic acid-2-magnesium phosphate was added, the appearance of deformed tail number was completely suppressed.
実施例２ 魚粉55％、サケ白子５％、クリルS.W.5％、イカ内蔵５
％、イカ肝油５％、大豆レシチン４％、バリン0.3％、
イソロシン0.2％、コーンスターチ５％、ミネラル混合
物５％、グルテン８％、ビタミンＣを除いたビタミン混
合物5.0％、ω３−HUFA1％、Ｌ−アスコルビン酸−２−
リン酸マグネシウム0.01％及び残分に大豆粉を添加して
100％としこの原料を粉砕後にミキサーで十分混合しカ
ルフォルニア ペレット ミル社製ペレットミル（内部
温度70〜100℃）で常法により平均粒径5mmのペレットに
加熱成型し100℃で送風乾燥させた。次にこの飼料を粉
砕し稚魚用飼料とした。高速液体クロマトグラム法（HP
LC法）でＬ−アスコルビン酸−２−リン酸マグネシウム
を測定したところ添加量の95％が残存していた。 Example 2 Fish meal 55%, salmon shirako 5%, krill SW 5%, squid 5
%, Squid liver oil 5%, soy lecithin 4%, valine 0.3%,
Isorosin 0.2%, corn starch 5%, mineral mixture 5%, gluten 8%, vitamin mixture 5.0% without vitamin C, ω3-HUFA 1%, L-ascorbic acid-2-
Add 0.01% magnesium phosphate and soy flour to the rest
The raw material was crushed to 100%, thoroughly mixed with a mixer, then heat-molded into pellets having an average particle size of 5 mm by a conventional method in a pellet mill manufactured by California Pellet Mill Co., Ltd. (internal temperature 70 to 100 ° C.), and dried by blowing at 100 ° C. Next, this feed was crushed to obtain a feed for fry. High Performance Liquid Chromatogram Method (HP
When L-ascorbic acid-2-magnesium phosphate was measured by the LC method), 95% of the added amount remained.
実施例３ 魚粉35％、コーンミール30％、マックコラム塩３％、ビ
タミン混合物5.0％、Ｌ−アスコルビン酸−２−リン酸
マグネシウム0.01％及び残分に大豆粉を添加して100％
としこの原料を粉砕後にミキサーで十分混合した後ウエ
ンガー社製エクストルーダーペレットミルによりクッカ
ー水分含量28％に調湿し蒸煮しエクストルーション成型
した。この成型物を２段式バンドドライヤーにより温度
120〜170℃で乾燥させ平均粒径100mmの水産用魚用エキ
スパンションペレット飼料を製造した。高速液体クロマ
トグラム法でＬ−アスコルビン酸−２−リン酸マグネシ
ウムを測定したところ添加量の96％が残存していた。Example 3 Fish flour 35%, corn meal 30%, Maccolum salt 3%, vitamin mixture 5.0%, L-ascorbic acid-2-magnesium phosphate 0.01% and soybean flour added to the residue to 100%
After crushing this raw material and thoroughly mixing it with a mixer, the moisture content of the cooker was adjusted to 28% by an extruder pellet mill manufactured by Wenger and steamed to form an extrusion. This molded product is heated by a two-stage band dryer.
It was dried at 120 to 170 ° C. to produce an expansion pellet feed for marine fish with an average particle size of 100 mm. When L-ascorbic acid-2-magnesium phosphate was measured by a high performance liquid chromatogram method, 96% of the added amount remained.
比較試験例２ 第２表に示すＬ−アスコルビン酸誘導体類0.1ミリモル
をそれぞれ実施例２のＬ−アスコルビン酸−２リン酸マ
グネシウムのみを除いた同じ組成の飼料に同じ製造方法
で成型した後粉砕し製造直後の飼料中のＬ−アスコルビ
ン酸誘導体類の残存率を測定した。次に室温で２週間放
置し同様に飼料中のＬ−アスコルビン酸誘導体類の残存
率を測定し、この飼料を平均体重2.6gのハマチ（モジャ
コ）に投与し３カ月間にわたって飼養試験を実施し試験
終了時にハマチの増重率、生存率、肝臓中のアスコルビ
ン酸濃度を測定し、総合評価としてアスコルビン酸誘導
体類を添加した飼料のハマチに対する有効性を調べた。Comparative Test Example 2 0.1 mmol of L-ascorbic acid derivatives shown in Table 2 was molded into a feed having the same composition except that only L-ascorbic acid-2 magnesium phosphate of Example 2 was formed by the same manufacturing method and then pulverized. The residual rate of L-ascorbic acid derivatives in the feed immediately after production was measured. Then, the mixture was allowed to stand at room temperature for 2 weeks, the residual ratio of L-ascorbic acid derivatives in the feed was measured in the same manner, and this feed was administered to Hamachi (Moyako) with an average body weight of 2.6 g, and a feeding test was conducted for 3 months. At the end of the test, the weight gain rate, survival rate, and ascorbic acid concentration in the liver were measured, and as a comprehensive evaluation, the effectiveness of the feed containing the ascorbic acid derivatives was investigated.
比較試験例３ 本発明のＬ−アスコルビン酸−２リン酸エステルの塩類
の養魚類に対するアスコルビン酸への酵素的変換活性を
確認するため第３表の魚類について以下の実験を行い活
性の存在することを確認した。 Comparative Test Example 3 In order to confirm the enzymatic conversion activity of the salts of the L-ascorbic acid-2 phosphate of the present invention into ascorbic acid for fish farming, the following experiments were conducted on the fish shown in Table 3 and the presence of the activity was confirmed. It was confirmed.
養殖魚類の肝臓と腸を新鮮な状態で摘出し臓器重量を測
定し、50倍の冷水を加えて冷温下でホモジナイズし上澄
液を取り1:1で混合しこれを酵素液とした。次に0.05％
重量のＬ−アスコルビン酸誘導体類を含むpH5.0、pH7.0
及びpH9.0のバッファー溶液8mlの入った試料瓶を35℃に
保ち、酵素液2mlを添加して35℃の水浴中で１時間放置
した後、異なるpHで反応させた酵素液を1:1:1で混合し
酵素反応を止める。解凍直後、２％メタリン酸溶液を加
えて50mlとした後、２％メタリン酸で更に50倍に希釈
し、その20μｌをHPLC分析し残存しているＬ−アスコル
ビン酸エステル類の濃度を求め反応前の濃度に対する加
水分解率を求めた。その結果50％以上の高い加水分解活
性が認められたものについては○を記入し、50％以下の
ものについては×を記入した。その結果全てのＬ−アス
コルビン酸−２−リン酸エステルの塩類に、試験した全
ての魚類の酵素液に対して50％以上の高い加水分解活性
が確認された。The liver and intestine of the cultured fish were removed in a fresh state, the weight of the organ was measured, 50 times cold water was added, and the mixture was homogenized under cold temperature, and the supernatant was collected and mixed 1: 1 to prepare an enzyme solution. Then 0.05%
PH 5.0, pH 7.0 containing by weight of L-ascorbic acid derivatives
And the sample bottle containing 8 ml of pH 9.0 buffer solution was kept at 35 ° C, 2 ml of enzyme solution was added and left in a water bath at 35 ° C for 1 hour, and then the enzyme solution reacted at different pH was 1: 1. Mix at 1: to stop the enzymatic reaction. Immediately after thawing, add 2% metaphosphoric acid solution to make 50 ml, dilute it further 50 times with 2% metaphosphoric acid, and analyze 20 μl of it by HPLC to determine the concentration of the remaining L-ascorbic acid ester before reaction. The hydrolysis rate with respect to the concentration of was determined. As a result, those with a high hydrolysis activity of 50% or more were marked with ○, and those with 50% or less were marked with ×. As a result, all the salts of L-ascorbic acid-2-phosphate ester were confirmed to have a high hydrolysis activity of 50% or more with respect to the enzyme solutions of all the tested fish.
発明の効果 Ｌ−アスコルビン酸−２−リン酸エステルの塩類は養魚
飼料に添加し飼料製剤化することができ、長期間の保存
に対して安定で広範囲の養殖魚類においてアスコルビン
酸活性を発揮することができる。そして、本発明の養魚
飼料用添加物および養魚飼料の使用により養殖魚類の成
長率の向上、へい死率の低下、品質の向上などの効果を
あげることができる。 EFFECTS OF THE INVENTION The salts of L-ascorbic acid-2-phosphate ester can be added to fish feed and formulated into feed, stable for long-term storage, and exhibiting ascorbic acid activity in a wide range of cultured fish. You can Further, the use of the fish feed additive and fish feed of the present invention can bring about effects such as improvement of growth rate of cultured fish, reduction of mortality and improvement of quality.
Claims (3)
リン酸エステルの塩類を含有することを特徴とするアス
コルビン酸活性を有する養魚飼料用添加物。1. L-ascorbic acid-2-as an active ingredient
An additive for feed for fish farming having ascorbic acid activity, characterized by containing salts of phosphoric acid ester.
ンザケ、アユ、アマゴ、ヤマメ、ハマチ、タイ、コイ、
ウナギ、フグ、ヒラメ、テラピヤ、アジ、シマアジ、マ
グロ、カツオまたはフナである特許請求の範囲１）の養
魚飼料用添加物。2. The cultivated fish is rainbow trout, hime salmon, chum salmon, coho salmon, ayu, amago, yamame trout, hamachi, Thailand, carp,
The additive for fish-feeding according to claim 1), which is an eel, a puffer fish, a flounder, a terrapia, a horse mackerel, a horse mackerel, a tuna, a skipjack or a crucian carp.
リン酸エステルの塩類を蛋白源を50％以上含有する飼料
に添加してなることを特徴とするアスコルビン酸活性を
有する養魚飼料。3. L-ascorbic acid-2-as an active ingredient
A fish feed having ascorbic acid activity, which is obtained by adding phosphate salts to a feed containing 50% or more of a protein source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61016739A JPH0693821B2 (en) | 1986-01-30 | 1986-01-30 | Additives for fish feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61016739A JPH0693821B2 (en) | 1986-01-30 | 1986-01-30 | Additives for fish feed |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10011455A Division JP2943785B2 (en) | 1998-01-23 | 1998-01-23 | Additives for fish farm powder feed and feed for fish farm |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62175142A JPS62175142A (en) | 1987-07-31 |
| JPH0693821B2 true JPH0693821B2 (en) | 1994-11-24 |
Family
ID=11924634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61016739A Expired - Lifetime JPH0693821B2 (en) | 1986-01-30 | 1986-01-30 | Additives for fish feed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0693821B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2257628A (en) * | 1991-06-20 | 1993-01-20 | Martin Richard Jaffa | Vitamin pill for fish |
| AU6046098A (en) * | 1997-02-07 | 1998-08-26 | Cultor Oyj (Corporation) | Process of enhancing growth and survival of aquatic organisms through water borne enrichment with stable vitamin c derivatives |
| JP2000302683A (en) * | 1999-04-23 | 2000-10-31 | Showa Denko Kk | Animal administration agent and raising method |
| CN105146077A (en) * | 2015-08-05 | 2015-12-16 | 贾红琴 | Chinese herbal medicine additive for aquaculture and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4924783A (en) * | 1972-06-29 | 1974-03-05 | ||
| JPS52102192A (en) * | 1976-02-24 | 1977-08-26 | Nippon Chemiphar Co | Feed for fish farming containing ascorbtc acidd22 sulphate |
| JPS594438B2 (en) * | 1976-05-06 | 1984-01-30 | カンサス・ステ−ト・ユニバ−シテイ・リサ−チ・フアンデ−シヨン | Process for producing phosphoric acid ester of ascorbic acid |
-
1986
- 1986-01-30 JP JP61016739A patent/JPH0693821B2/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| Ｐｒｏｇ．Ｆｉｓｈ−Ｃｕｌｔ．４７（１）（１９８５）Ｐ．５５−５９ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62175142A (en) | 1987-07-31 |
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