JPH0690620A - Inoculation into culture bag for mushroom fungus - Google Patents

Abstract

PURPOSE:To provide an efficient and economical method for inoculating into a culture bag widely used for culturing SHIITAKE (mushroom), P. Grifola, etc. CONSTITUTION:A nutritive medium for culture of a mushroom fungus is charged into a bag composed of a plastic film and having a porous sheet part capable of cutting off various germs on the side surface and the opening part of the bag is sealed. The culture bag for mushroom fungi is subsequently sterilized and a mushroom fungus suspension is injected through plural positions in the side surface of the bag by using an injection syringe. A molten plastic is then sprayed on the needle holes made by the injection syringe so as to weld the holes.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for inoculating a fungus culture bag, which is widely used for cultivating shiitake mushrooms, maitake mushrooms and the like.

[0002]

2. Description of the Related Art Mushrooms can be cultivated not only by traditional methods using a wood but also in a plastic bottle or a bag made of plastic film by using a medium containing wood powder such as sawdust and rice bran as main components. There is a way. In particular, a bag made of a plastic film has been widely used in recent years because it is lightweight and easy to handle. As a typical example, Jikho Sho 57-
No. 22518 proposes a bag of cultivated fungi of fungi, in which a part of the plastic film is a porous plastic film having a pore size of 0.02 to 0.4 μm. The porous plastic film with a pore size of 0.02-0.4μ is
It prevents the invasion of bacteria without obstructing the circulation of air necessary for the growth of mushrooms.

The bag is filled with nutrients for culturing the fungus (mainly wood flour such as sawdust and rice bran) and sterilized by heating, and then the fungus is inoculated. In the past, after heat sterilization, the bag was opened and powdered fungi were sprayed with a spoon, but with this method it is difficult to evenly spray a certain amount of fungi, and when the bag is inoculated Since it has an open mouth, it cannot prevent the invasion of outside air containing miscellaneous bacteria, and is labor-intensive, which makes it unsuitable for mass production.

In order to improve the above problems, the bag was filled with a nutrient for culturing the fungus, the opening of the bag was sealed and sterilized, and then the fungus was suspended using a syringe. A method of injecting a liquid has been proposed. For example, Japanese Patent Publication No. 4-308
In No. 06, sol-shaped inoculum was placed in a bag-shaped container and sterilized in a sterile atmosphere by injecting an injection dispenser obliquely diagonally to the container to inoculate almost uniformly. Disclosed is a method for inoculating a mushroom seed culture. Then, it is stated that a label is attached to seal the puncture hole of the injection dispenser and the seal is aseptically sealed.

[0005] Mushrooms propagate hyphae sufficiently in the nutrients and then grow mushrooms on the surface of the nutrients. In order to promote the growth of hyphae in the nutrient group, it is desirable to inject the fungi in a plurality of locations, for example, four locations on the top, bottom, left and right. However, when injected into such a large number of places, it is very troublesome to label each place. When using an automatic labeler, it is necessary to install the number of automatic labelers corresponding to the number of injection points, which increases the burden of equipment costs.

[0006]

DISCLOSURE OF THE INVENTION An object of the present invention is to provide an efficient and economical method for inoculating a fungus culture bag, which is widely used for cultivating shiitake mushrooms, maitake mushrooms and the like. To do.

[0007]

The method for inoculating a fungus culture bag according to the present invention comprises a bag made of a plastic film and a side part of which is formed of a bacteria-blocking porous sheet. Using a syringe, inject the liquid in which the fungus is suspended into multiple locations on the side of the bag that is filled with nutrients for culturing the fungus and sealed the opening of the bag and then sterilized. Then, the molten plastic is injected and fused to the needle hole of the syringe.

It is preferable that the porous sheet for preventing germs used in the bag for cultivating mushrooms is one that blocks invasion of germs but has excellent air permeability. Specifically, the maximum pore size is 0.02 × 0.
2 micron to 0.04 x 0.4 micron microporous polypropylene film (trade name: Ceraguard;
It is desirable to use a product manufactured by Celanese Company, USA.

As the sterilization treatment, normal pressure steam sterilization using steam at about 100 ° C. or high pressure steam sterilization using steam at 120 to 125 ° C. is generally performed. The treatment time depends on the size of the bag and therefore the amount of nutrients used for cultivating the fungus, but when the amount of nutrients is 2.5 Kg, it is 7 in normal pressure steam sterilization including the heating and cooling time. The standard time is 5 hours for autoclaving.

The structure of the present invention will be described with reference to FIG. 1. Reference numeral 1 (1A, 1B, 1C, 1D) is a bag made of a plastic film, and a part of the side surface is a bag in which a bacteria blocking porous sheet is formed. A fungus culture bag, which is sterilized after being filled with a nutritional group for culturing fungi and sealing the opening of the bag, is placed on the conveyor 2. In FIG. 1, the conveyor 2 moves intermittently from left to right (in the arrow direction). 1
A, 1B, 1C, and 1D show the positions where the fungus culture bag 1 moved along with the intermittent movement of the conveyor 2. Symbol 3
Is a partition provided on the conveyor 2 for positioning the mushroom culture bag 1.

As shown in FIG. 1, the bag of the mushroom culture at 1A is
It is a non-inoculated strain in a state in which it is sterilized after being filled with a nutrient group and the opening of the bag is sealed. When it comes to the position of 1B, a plurality of places on the side surface of the mushroom culture body bag (for example, four places on the top, bottom, left and right
(In Figure 1, the lower part is hidden by the upper part and cannot be seen.)
The injection needle 4 of the syringe is inserted into and the liquid in which the mushroom fungus is suspended is injected. FIG. 2 is a side view of the mushroom culture bag at the position 1B. Next, the injection needle is pulled out and transferred to the position 1C, but the bag has the needle hole 5. The needle hole of the injection needle has a diameter of about 5 mm. At the position of 1C, the molten plastic is injected and fused from the nozzle 6. Figure 1
It is the figure which looked at the mushroom culture bag in the position of C from the side.
The injected molten plastic is fused and solidified on the surface of the bag to form a film 7, which closes the needle hole 5. That is what is shown in 1D. FIG. 4 is a side view of the fungus culture bag at the position 1D in FIG.

Molten plastic injectors have been applied for a variety of purposes. For example, a commercially available hot melt applicator system (manufactured by Nordson Co., Ltd.) can be used. Since a plurality of nozzles can be connected to one plastic heating / melting apparatus main body in such an apparatus, the system shown in FIG. 1 requires only one main body and requires a small equipment cost.

As the material of the plastic to be melted and jetted, the same material as the material of the bag or a material having a good affinity with the material of the bag is used.

The temperature of the portion where the molten plastic is sprayed rises, but since it is local and temporary, it does not affect the growth of the injected fungi.

[0015]

EFFECTS OF THE INVENTION It is possible to efficiently inoculate a fungus culture bag, which is widely used for cultivation of shiitake mushrooms, maitake mushrooms and the like.

[Brief description of drawings]

FIG. 1 is a diagram for explaining an embodiment of the present invention.

FIG. 2 is a side view of the mushroom culture bag at the position 1B in FIG.

FIG. 3 is a side view of the fungus culture bag at the position 1C in FIG.

FIG. 4 is a side view of the fungus culture bag at the position 1D in FIG.

Claims (1)

[Claims] 1. A bag-shaped bag made of a plastic film, part of the side surface of which is composed of a bacteria-blocking porous sheet, is filled with a nutritional group for culturing fungi, and the opening of the bag is sealed. At multiple locations on the side of the post-sterilized mushroom culture bag,
A method for inoculating a fungus culture bag, which comprises injecting a liquid in which a fungus is suspended with a syringe and then spraying and melting molten plastic into a needle hole of the syringe.