JPH0662662A - Method for artificially culturing mushrooms - Google Patents

Abstract

PURPOSE:To use a completely sterilized medium in artificial culture of mushroom. CONSTITUTION:A proper sterile solid medium is mixed with a cell of mushroom subjected to liquid culture and a culture solution containing given nutritive components and the mixture is used as a mushroom bed to culture mushrooms. Consequently, each process of a fungus solution (fungus culture tank 4), a culture solution (culture solution tank 5), a solid medium (solid medium storage tank 1, sterilizer 2 and cooler 3) and inoculating and packing (automatic packing device) can be constituted independently. This method can deal with production of mushroom bed of various kinds of mushrooms by changing conditions of each process, rapidly permeates spawn to the medium, uniformly grow mycelia of the whole mushroom beds, quickly produces fruit bodies and has a high early yield.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention is mainly applied to artificial cultivation of mushrooms in which a mushroom cultivating farm is supplied with a fungus bed, and the fungus is grown from the fungus bed to harvest the fungus, particularly It relates to the production of bacterial beds.

[0002]

2. Description of the Related Art Container cultivation such as nameko, shimeji mushroom, enoki mushroom, oyster mushroom, etc. is a film, glass,
Fill a container made of resin etc. with a medium consisting mainly of sawdust,
A fungus bed, which is prepared by inoculating separately grown inoculum (medium grown in ogre scrap medium) on this medium, is supplied to the mushroom farmer, and the fungus bed, which is the fruiting body of the fungus, is grown. It is to be harvested.

[0003] In particular, a technique is known in which, as the above-mentioned inoculum, cells that have been liquid-cultured without being cultured in an agar scrap medium are used. For example, in Japanese Patent Laid-Open No. 59-154920, the liquid culture is used as an inoculum, and the media such as sapwood and old wood are soaked in the culture solution of the mushroom mycelia to attach the mycelia to the surface of the medium. Japanese Patent Application Laid-Open No. 59-25620 discloses a technique of inoculating a bacterial cell culture solution into a culture medium such as sawdust chaff.

[0004]

By the way, generally, the nutrients required for cultivating mushrooms are added to the medium to increase the incidence of fruiting bodies of mushrooms. However, the presence of fungi other than the fungus in the medium inhibits the growth of the fungus and reduces the production of fruiting bodies, or makes the harvest impossible at all.

In order to avoid them, generally, bacteria in the medium are removed by heating. However, since the medium is solid, the bactericidal effect is poor. Further, for complete sterilization, a high temperature and long time treatment is required, and as a result, the organic matter as a medium component undergoes heat denaturation, which causes a growth inhibition of the fungus when used as a medium as it is. Therefore, the present invention proposes a method for artificially cultivating mushrooms that can use a completely sterilized medium.

[0006]

[Means for Solving the Problems] A method for cultivating mushrooms according to the present invention is a method in which a liquid-cultured fungus body and a culture solution containing predetermined nutrients are mixed in an appropriate sterilized solid medium, and the mixture is mixed. It is characterized in that mushrooms are cultivated as a fungus bed, or a mixed solution of fungi cells that have been liquid-cultured and a culture solution containing predetermined nutrients, and added dropwise to an appropriate sterilized solid medium. Inoculation or mixed inoculation is performed, and mushrooms are cultivated using this inoculum as a fungal bed.

[0007] Furthermore, the liquid-cultured fungus bodies and a culture solution containing predetermined nutrients are inoculated drop-wise or mixed-inoculated into a suitable sterilizing carrier with a single or a mixture thereof, and the inoculum is appropriately determined. It is characterized by being inoculated into a sterilized solid medium and cultivating mushrooms.

[0008]

[Function] By completely sterilizing the medium or the carrier, it is possible to prevent the contamination of other bacteria, and all nutrients necessary for the growth of the mushroom are contained in the culture solution. Since it is not necessary to obtain a nutrient source, an inorganic substance can be used in the medium, and the conditions for pretreatment such as sterilization can be arbitrarily set, and the pretreatment can be facilitated. Furthermore, liquid culture broth,
It is possible to separate the production process of the liquid medium, the medium or the carrier, etc., and it becomes possible to easily control the initial conditions of culture such as water, PH, nutrient sources, and the inoculation amount of bacteria.

[0009]

EXAMPLES Next, examples of the present invention will be described. A device for carrying out the method of the present invention, which is used in a mixed inoculation system of a mixed solution into a medium, will be described. As illustrated in FIG. 1, the embodying apparatus is composed of a solid medium producing section, an inoculum producing section, and a bacterial bed producing section. The solid medium production section comprises a solid medium storage tank 1 for shavings and the like, a sterilizer 2 and a cooler 3, which are connected in series, and the solid medium storage tank 1 stores the shavings and other inorganic materials. The solid medium is supplied to the sterilizer 2 via the.

The sterilizer 2 is provided with a solid culture medium transport mechanism 22 inside a cylindrical body 21, and a superheated steam supply section 23 and a superheated steam discharge section 24. Indirect heat sterilization is performed. The cooler 3 conveys the solid medium that has undergone overheat sterilization,
It is cooled by using cold air, cold water, or both.

The inoculum production section is composed of a bacterial culture tank 4 and a culture solution tank 5. The bacterial culture tank 4 is provided with a sterile air supply section 42 having an air filter 41 and an agitator 43 in the tank.
The liquid culture of the fungus is performed, and the culture solution tank 5 is a storage tank for the culture solution containing the nutrients necessary for the growth of the fungus.

The bacterial bed production section is composed of a mixing device 6 and an automatic packaging device 7. The mixing device 6 includes the solid medium from the cooling device 3, the culture solution from the culture solution tank 5, and the bacteria culture tank. The fungus body is supplied from No. 4 and mixed inoculation of the fungus is carried out on the solid medium. In addition, the automatic packaging device packages the solid medium after the inoculation into an appropriate amount in a film, and supplies it to each cultivating farmer in this packaged state.

Next, the implementation of the method of the present invention using the above-described implementation apparatus will be described. Examples are Shiitake strain 18-7.
315. The strain is once subjected to flask culture under the conditions shown in Table 1, and further subjected to deep liquid culture in the bacterial culture tank 4 while supplying the liquid medium shown in Table 2.

[0014]

[Table 1]

[0015]

[Table 2]

The culture medium having the composition shown in Table 3 is stored in the culture medium tank 5.

[0017]

[Table 3]

[0018]

[Table 4]

Further, the solid medium has the composition as shown in Table 4, is sterilized and cooled through the sterilizer 2 and the cooler 3, and is sent to the mixing device 6, where 0.1 cell liquid per 100 g of the solid medium.
~ 1.0 g (0.5-5 mg of cells; dry state) and 100 g of culture solution were mixed to adjust the water content to 65%, and 2.
Each 5 kg is packed and shipped as a cultivation fungus bed.

Under the same conditions (65% water, temperature 25 ° C.), a fungus bed employing the above-mentioned method of the present invention and an inoculum of cultivated shavings of approximately the same amount were inoculated on a conventional shaving medium.
When it was cultivated in, the method of the present invention required 6 days for the hyphae to spread to the fungal bed, while the conventional method required 30 days.

The occurrence of fruiting bodies was recognized in 33 days, but the conventional method required 80 days. The yield of the fruiting body is shown in Table 5.

[0022]

[Table 5]

The method of the present invention is not limited to the above-mentioned embodiment. The present invention is not limited to the solid medium in which various bacteria are sufficiently eliminated by sterilization, the inoculum of liquid culture widely penetrating into the solid medium, and the nutrients of the bacteria. If the combination of
In order to achieve the object of the present invention, a sterilized solid medium (appropriate inorganic matter or organic matter other than sawdust may be used), kind of mushroom to be cultivated, liquid culture means and culture solution composition, further inoculation means, etc. Can be set arbitrarily.

[0024]

EFFECTS OF THE INVENTION The present invention comprises a fungus bed formed by inoculating an appropriate sterilized solid medium with a fungus body cultivated in liquid as described above and a culture solution containing a predetermined nutrient component. Since each process of bacterial solution (fungi cells), culture solution, solid medium, and inoculation packaging can be configured independently, it is possible to adapt to the production of various types of mushroom beds by changing the conditions of each process. In addition, since inoculation of the inoculum into the culture medium is speeded up and the growth of hyphae in the whole bacterial bed is uniform, the fruiting body is rapidly generated, the initial yield is large, and the production plan of the cultivating farmer is easy.

[Brief description of drawings]

FIG. 1 is a simple configuration diagram of an apparatus for carrying out a method of the present invention.

[Explanation of symbols]

 1 Solid medium storage tank 2 Sterilizer 3 Cooler 4 Bacteria culture tank 5 Culture liquid tank 6 Mixing device 7 Automatic packaging device

Claims (4)

[Claims] 1. A liquid-cultured fungus body and a culture solution containing predetermined nutrients are mixed in an appropriate sterilized solid medium,
A method for artificially cultivating mushrooms, which comprises cultivating mushrooms using this mixture as a fungal bed. 2. A mixture of a liquid-cultured fungus body and a culture solution containing a predetermined nutritional component is inoculated into a suitable sterilized solid medium by drop inoculation or mixed inoculation, and the inoculum is used as a fungus bed. A method for artificially cultivating mushrooms, which comprises cultivating. 3. A liquid cultivated fungus body and a culture solution containing a predetermined nutrient component are individually or mixedly inoculated into an appropriate bactericidal carrier by drop inoculation or mixed inoculation, and the inoculum is appropriately inoculated. A method for artificially cultivating mushrooms, which comprises culturing mushrooms by inoculating a sterilized solid medium. 4. The method for cultivating mushrooms according to any one of claims 1 to 3, wherein a mixture of organic matter and inorganic matter such as inorganic matter or sawdust is used as a sterilizing medium. Method.