JPH0648934A - Skin external agent - Google Patents

Abstract

PURPOSE:To provide the skin external agent containing a specific plant extract as an active ingredient and useful as a chromatosis therapeutic agent or skin aging-preventing agent. CONSTITUTION:The skin external agent contains the extract of at least one kind of plants selected from a group consisting of Benincasa cerifera Savi seeds, Anethum graveolens L., Pinpinella anisum L., dried Boschniakia rossica and tarragon as an active ingredient, and has an excellent melamine production- inhibiting action, an excellent collagen production-stimulating action, and further a cell multiplication-accelerating action. The addition of a whitening agent selected from a group consisting of kojic acid, ascorbic acid, hydroquinone, liquiritin or their derivatives and a placenta extract synergistically increases the melamine production-inhibiting action, and the addition of a collagen production-stimulating agent selected from a group consisting of a Salvia miltiorrhiza Bunge extract, calcium ascorbate phosphate, adenosine-3',5'-cyclic monophosphate (derivative), guanosine-3',5'-cyclic monophosphate (derivative), gamma-amino butyric acid and retinoic acid synergistically increases the collagen production-stimulating action.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin external preparation containing a plant extract as an active ingredient, and more specifically, at least one plant extract selected from capsicum, dill, anise, salmon roe, and tarragon. High safety characterized by containing as an active ingredient,
The present invention relates to a skin external preparation having excellent melanin production suppressing action and collagen production promoting action, and further having a cell growth promoting action.

[0002]

2. Description of the Related Art It is known that spots such as spots and freckles appearing on human skin are mainly caused by deposition of Eumelanin. It is also well known that various types of whitening cosmetics for suppressing the production of melanin, which causes stains and freckles, and for whitening the entire skin have been studied for a long time. As examples thereof, it has been attempted to blend hydrogen peroxide, peroxides such as zinc perborate, and further vitamin C, cysteine, colloidal sulfur and the like into cosmetics. However, the actual situation is that none of them has been satisfactory in terms of preservability and whitening effect.

Under such circumstances, various attempts have been made to utilize the plant extract as an active ingredient of fair skin cosmetics.
Conventional plant extracts are highly cytotoxic, and when applied to the human body, safety such as skin irritation often poses a problem.
For example, Touki extract, which is a plant extract of the Apiaceae, has a melanin production inhibitory action and is also known to be used as a skin external preparation, but this plant extract has not only cytotoxicity but also melanin-producing cells. However, there are problems such as not exhibiting an effective melanin production suppressing effect,
The whitening effect was also inadequate.

The present inventors have been studying the topical melanin production external preparations such as whitening cosmetics for many years to remove spots such as spots and freckles appearing on human skin and enhance the whitening effect of the entire skin. I have just been researching. Among these, we found that melanin, carotene, blood flow (oxidized and reduced hemoglobin), and skin thickness and transparency are factors that are involved in normal human skin color. It reflects, absorbs, and scatters depending on the factor, and becomes the color of the skin. Among these, it was clarified that melanin, especially brown or black eumelanin, was the most important factor for pigmentation, and melanin production was suppressed. I have been searching for a substance to suppress.

In the study, it was found that kojic acid (5-oxy-2-oxymethyl-γ-pyrone) and its derivatives exhibited a very excellent melanin production inhibitory action, and for example, Japanese Patent Publication No. 56-18569. Gazette, JP-A-5
4-92632, JP-A-56-79616, JP-A-56-77272, and JP-A-56-77.
76, JP-A-56-7710, JP-A-56
As disclosed in Japanese Patent Publication No. 20330 and Japanese Patent Publication No. 63-24968, various inventions relating to whitening cosmetics and external preparations containing these as active ingredients have been proposed.

Further, the present inventors have been earnestly researching the prevention (prevention) of human skin aging, especially the prevention (prevention) of wrinkles. Traditionally,
As a skin aging preventive agent, various moisturizing agents, antioxidants, blood circulation promoting agents, cell growth promoting agents and the like have been proposed and developed. However, they do not directly eliminate the factor of wrinkles, and the fact is that they do not show remarkable effects even after use. The present inventors have noticed that the wrinkle factor is mainly the change of dermal collagen fibers due to aging, that is, the decrease in the amount of collagen,
The above problems have been solved by enhancing the production of collagen and promoting cell growth. When we extensively searched for substances that enhance the production of collagen, we were able to find effective activities in some plant extracts.

These external preparations for skin are used in the form of products such as creams, lotions, emulsions, packs, lotions, ointments and poultices, all of which show a large melanin production inhibitory effect and excellent whitening properties. It is used as an effective cosmetic or an external preparation, and is also promising as a wrinkle preventive agent and thus an aging preventive agent.

However, these cosmetics may be required to be absorbed through the skin after use, that is, to have excellent transdermal absorbability. In particular, cosmetics containing kojic acid or a kojic acid derivative as an active ingredient do not cause any problem in normal use, but absorbability immediately after use, that is, initial transdermal absorbability is rather slow. There is a tendency to improve such a tendency, it is preferable that it be absorbed into the skin promptly after use, for example,
In applications such as ointments, pasta agents, poultices, creams, emulsions, lotions, and essences, it is possible to add a transdermal agent to the external preparation in order to further improve the transdermal absorbability. I've been told.

Examples of the absorption aid include C10 to C
14 fatty acid esters, fatty acids, PEG (M <1,0
00), alcohols, etc. are known, but many of these absorption aids have skin irritation, and the amount used in the formulation is limited, so the desired drug effect is obtained. The reality was that I could not demonstrate it.

[0010]

SUMMARY OF THE INVENTION The object of the present invention is, therefore, the drawbacks of the above plant extracts, the characteristics of conventional whitening agents such as kojic acid and kojic acid derivatives, and the conventionally known wrinkles (aging). An object of the present invention is to provide an external preparation for skin which improves the effect of a preventive agent, is highly safe, and is excellent in the effect of suppressing melanin production or the effect of promoting collagen production, and further in the effect of promoting cell proliferation.

[0011]

As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that specific plant extracts, namely, pepper, dill, anise, salmon roe, and tarragon, have been problematic. It was found that they had low cytotoxicity and had an excellent melanin production inhibitory action and collagen production promoting action. Furthermore, by combining at least one selected from those plant extracts with kojic acid, ascorbic acid, hydroquinone, liquiritin and their derivatives, and a conventionally known whitening agent such as a placenta extract, the melanin production inhibitory action is synergistic. , Ginseng, magnesium ascorbyl phosphate, dibutyryl-3'5'-adenosine cyclic monophosphate sodium salt, γ-aminobutyric acid, retinoic acid, ascorbic acid higher fatty acid ester, and mammalian whey. By combining with the conventionally known collagen production enhancer,
The present invention has been completed by finding that synergistic enhancement of collagen production is enhanced.

[0012] That is, according to the present invention, there is provided a skin external preparation characterized in that at least one plant extract selected from the group consisting of capsicum, dill, anise, salamander and tarragon is used as an active ingredient. . Further, according to the present invention, the plant extract, kojic acid, ascorbic acid, hydroquinone, liquiritin or derivatives thereof, and a skin external preparation using at least one skin-whitening agent selected from the group consisting of placenta extracts, and Those having an action of promoting collagen production such as Danshen, for example, magnesium ascorbyl phosphate, dibutyryl-
Provided is an external preparation for skin in which 3'5'-adenosine cyclic monophosphate sodium salt, γ-aminobutyric acid, retinoic acid, higher ascorbic acid fatty acid ester, and at least one collagen production enhancer such as mammalian whey are used in combination. It In this case, a skin external preparation using the plant extract together with a skin-whitening agent and a collagen production promoting agent is also provided. In capsicum, dill, anise, salamander, and tarragon,
Regarding the melanin production inhibitory action, cell proliferation action and collagen production promoting action, five kinds of plants also have strong and effective activities.

[0013]

DETAILED DESCRIPTION OF THE INVENTION The present invention is described in detail below. The chili pepper used in the present invention is also called as a postcard, and is a seed of an annual herbaceous gourd of the genus Cucurbitaceae, which is provided for food as a fruit, and its extract is, for example, seeds or fruit shreds. , And alcohols such as methanol and ethanol, polar organic solvents such as acetone, and a mixture thereof in any proportion, or nonpolar organic solvents such as diethyl ether acetate and hexane, oils and fats of animals and plants, and any of them. It can be obtained by extraction with a mixed solution of the ratio or by concentration by means such as reduced pressure. Further, the activity of suppressing melanin production and promoting collagen production in winter melon is high in seeds and their peripheral portions, but the site is not particularly limited, and for example, effective activity is also obtained in pulp and skin. be able to.

Dill (inondo) and anise are both plants of the genus Anethum and the genus Pimpinella, which are actually used for flavoring and aromatizing various dishes. The extract is, for example, seed or fruit,
Water, alcohols such as methanol and ethanol, polar organic solvents such as acetone, and mixtures thereof in arbitrary proportions, or nonpolar organic solvents such as diethyl ether and hexane, oils of animals and plants, etc. and their arbitrary proportions. It can be obtained by extraction with a mixed solution or concentration by means such as reduced pressure.

[0015] Cistanche
It is a plant of the genus and is an annual parasitoid that grows northwest of China, in Inner Mongolia, and north of central Japan in Japan. The whole plant is also known as juyo and taigay, and has been used as a tonic and tonic drug. . The extract is, for example, seeds or leaves, water, alcohols such as methanol and ethanol, polar organic solvents such as acetone, and a mixture thereof in any proportion, or non-polar organic solvents such as diethyl ether and hexane, animals, plants. It can be obtained by extracting with the oils and fats of the above and a mixed solution of them at an arbitrary ratio, or concentrating by means such as reduced pressure.

Tarragon is a plant of the genus Artemisia of the Asteraceae family, and dried tarragon leaves are often used as spices in French cuisine. The extract, for example, seeds or leaves, water, alcohols such as methanol and ethanol, polar organic solvents such as acetone,
And by extracting with a mixture of them in any proportion, or with a nonpolar organic solvent such as diethyl ether or hexane, an oil or fat of animals or plants, and a mixture of them in any proportion, or by concentrating by means such as reduced pressure. Obtainable.

Further, these extracts are mixed with various absorbents such as hydroxyapatite, activated carbon and ion exchange resins,
A purified active fraction or a concentrated product thereof can be used by an operation such as ultrafiltration, distribution, centrifugation, or column chromatography. Especially for red pepper, a fraction having a significantly enhanced cell growth promoting effect can be obtained by, for example, reverse osmosis membrane treatment.

These plant extracts alone have excellent melanin production inhibitory action and collagen production promoting action, and further, they have a fair white color such as kojic acid, ascorbic acid, hydroquinone, liquiritin and their derivatives, and placenta extract. When used in combination with an agent, it has a melanin production inhibitory effect, and also has an effect of inhibiting danshen, magnesium ascorbyl phosphate, dibutyryl-3′5′-adenosine cyclic monophosphate sodium salt, γ
-When used in combination with an anti-wrinkle (aging) preventive agent such as aminobutyric acid, retinoic acid, higher ascorbic acid fatty acid ester, and mammalian whey, the action of promoting collagen production becomes remarkable. When used in combination with both a skin-whitening agent and an anti-wrinkle agent, they bring about a synergistic effect with respect to each of the melanin production suppressing action and the collagen production promoting action. Although its mechanism of action is not yet clear, for example, with respect to melanin production, in addition to the enzyme inhibitory action of the enzyme tyrosinase involved in melanin production and the action of suppressing the production of melanin polymer as seen in kojic acid, these plant extracts are Since it has an action of suppressing the production of the enzyme tyrosinase itself in melanin-producing cells, it seems that a synergistic effect can be achieved.

The kojic acid has the formula (1)

[Chemical 1] A pure product of 5-oxy-2-hydroxymethyl-γ-pyrone represented by the above, a fermentation solution containing kojic acid as a main component, which is obtained by culturing a strain having a kojic acid-producing ability, and a concentrated solution of the fermentation solution, Also, a product obtained by extracting kojic acid from the fermentation liquid and crystallizing it is used.

Examples of such strains having the ability to produce kojic acid include Aspergillus albus, Aspergillus tamari, Aspergillus nubuus, Aspergillus flavus, Aspergillus wenci, Aspergillus glaucus, Aspergillus clavatus, Aspergillus fumigatus, Aspergillus strains such as Aspergillus digantas, Penicillium
Strains of the genus Penicillium such as Dalley, strains of the genus Escaria such as Escherichia coli, strains of the genus Acetobacter such as Acetobacter aceti, Acetobacter gluconicus, Acetobacter xylinum, Gluconobacter
Strains of the genus Gluconobacter, such as Rosius and Gluconobacter gurnicus, are preferably used.

The medium composition of these strains is as follows:
Usually, sucrose, sucrose, fructose, glucose, starch, maltose, glycerin, mannitol, rhamnose,
Carbon sources such as xylose, gluconic acid, arabinose, dihydroxyacetone, inosit, lactose, and ethanol are about 2 to 15% (wt%, the same applies hereinafter), ammonium sulfate, polypeptone, sodium nitrate, baker's yeast extract, brewer's yeast extract, etc. Nitrogen source is about 0.1 to 1
%, 0.01 to 0.05% of magnesium source such as magnesium sulfate, 0.01 to 0.1% of phosphorus and potassium source such as potassium monohydrogen phosphate and potassium dihydrogen phosphate, and other ferric sulfate and chloride. An inorganic salt such as ferric iron, sodium chloride or calcium chloride having a concentration of about 0.001 to 0.005% may be used.

Examples of the kojic acid derivative used in the present invention include 2-methoxymethyl-hydroxy-4H-pyran-4-one, 2-ethoxymethyl-5-hydroxy-4H-pyran-4-one and 2-benzo. Ruyloxymethyl-5-hydroxy-4H-pyran-4-one, 2-
Cinnamoyloxymethyl-5-hydroxy-4H-pyran-4-one, 2-phenoxymethyl-5-hydroxy-4H-pyran-4-one, kojic acid glycoside, or
Formula (2)

[Chemical 2] An esterified product of kojic acid represented by the formula (wherein R is a saturated or unsaturated aliphatic hydrocarbon group) is exemplified.
In the formula, the saturated or unsaturated aliphatic hydrocarbon group represented by R is, for example, a saturated or unsaturated aliphatic carboxylic acid.

Examples of the saturated aliphatic carboxylic acid include acetic acid, propionic acid, n-valeric acid, iso-valeric acid, methylethylacetic acid, trimethylacetic acid, caproic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, and undecyl. Acids, lauric acid, tridecylic acid, myristic acid, pentadecyl acid, palmitic acid, margaric acid, stearic acid, non-derelic acid, arachidic acid, lignoceric acid, etc. are used, but in order to reduce the skin irritation of acid, C8 is used. The above saturated aliphatic carboxylic acids, especially C14
It is preferable to use a saturated aliphatic carboxylic acid of -20.
The use of the aliphatic carboxylic acid having a carbon number of more than C20 is not particularly limited, but it is extremely difficult to obtain the aliphatic carboxylic acid, which is not preferable in terms of production cost.

In addition to these saturated aliphatic carboxylic acids, for example, linoleic acid, linolenic acid, maleic acid,
Unsaturated aliphatic carboxylic acids such as fumaric acid, olefinic acid, and arachidonic acid can be used without any special limitation.

The kojic acid glycoside is obtained by stabilizing a kojic acid molecule by binding a saccharide to the -CH ₂ OH group at the 2-position of kojic acid, which is represented by the following formula (3): It has a structural formula shown by.

[Chemical 3]

In the formula (3), R is a 6-carbon saccharide, a 5-carbon saccharide, an amino acid, a disaccharide or a trisaccharide. Examples of the 6-carbon saccharide include glucose, galactose, mannose,
Fructose, sorbose and the like, the five-carbon sugars include ribose, arabinose, xylose, liquirice, xylulose, and the like, the amino sugars such as glucosamine, mannosamine, and galactosamine, and the disaccharides such as Maltose, lactose, cellobiose, sucrose and the like, and the trisaccharides include, for example, maltotriose,
Examples include cellotriose.

The kojic acid glycoside of the present invention can be produced by any of the synthetic method, the enzymatic method and the culturing method, and any of them can be used. However, considering the productivity and economical efficiency, etc. For example, kojic acid glycosides produced by the enzymatic method or the culture method are preferably used. Generally, it is synthesized by utilizing a transglycosylation reaction of an enzyme such as amylase, phosphorylase and lysozyme, or unreacted at the 1-position of the sugar.
It can be produced by chemically bonding an OH group and kojic acid.

As the ascorbic acid derivative, in addition to known derivatives, for example, glycosides such as ascorbic acid glucoside, L-ascorbyl palmitate, L-ascorbyl isopalmitate, L-ascorbyl dipalmitate, and L-ascorbyl diisopalmitate are used. -Ascorbyl, L-ascorbyl stearate, L-Ascorbyl isostearate, L-Ascorbyl distearate, L-Ascorbyl diisostearate, L-Ascorbyl myristate, L-Ascorbyl isomyristate, L-Ascorbyl dimyristate, Diisomyristin Acid L-ascorbyl, 2-ethylhexanoic acid L-ascorbyl, di-2-
L-ascorbyl ethylhexanoate, L-ascorbyl oleate, L-ascorbyl dioleate, etc.
Ascorbic acid alkyl ester; L-ascorbic acid phosphoric acid ester such as L-ascorbic acid-2-phosphoric acid ester and L-ascorbic acid-3-phosphoric acid ester;
L-ascorbic acid 2-sulfate, L-ascorbic acid 3-sulfate and other L-ascorbic acid sulfates; their alkali metal salts such as sodium and potassium; their alkaline earth metals such as calcium and magnesium Salt and the like.

Examples of the hydroquinone derivative include arbutin. Examples of the liquiritin derivative include liquiritin-α-glucoside in which glucose is α-bonded to liquiritin, liquiritin-α-maltoside, and the like.

Further, in recent years, many have been proposed as those having an action of promoting collagen production, preventing wrinkles and preventing skin aging. For example, Danshen extract, fractionated product (JP-A-2-290805), ascorbic acid higher fatty acid ester (JP-A-2-304012),
Mammalian whey (JP-A-3-20206), guanosine 3′-5′-cyclic compound, etc. (JP-A-63-1835)
No. 35), ascorbic acid-2-sulfuric acid, etc.
0-78913), γ-aminobutyric acid and derivatives thereof (Japanese Patent Publication No. 62-20165), alicyclic compounds and the like (Japanese Patent Publication No. 62-20165), adenosine-
3'-5'-cyclic compound, sodium alginate sulfate,
Ascorbic acid magnesium phosphate, vitamin A, vitamin A acid and derivatives thereof and the like can be mentioned.

In the present invention, the amount of the above-mentioned active ingredient, that is, the extract having the action of suppressing melanin production and enhancing the production of collagen or the fractionated and purified product thereof, is a cream,
In any case where it is used as a cosmetic such as lotion, milky lotion, pack, lotion, essence, ointment, poultice, and pasta and an external preparation, it is 0 in total or in combination of them. 0.001 to 10.0% by weight, preferably 0.01 to 5.0% by weight. In addition, conventionally known whitening agents, for example,
Extracts such as kojic acid, ascorbic acid, hydroquinone, liquiritin and their derivatives, plants containing them, and extracts such as culture solutions, concentrates, fractionated products, and placenta extracts can be used as the extract of the present invention 1 0.01 to 10.0 weight ratio, preferably 0.05 to 5.0 weight ratio. Conventionally known collagen production-promoting substances (wrinkles, anti-aging agents), for example, Danshen, magnesium ascorbate phosphate, adenosine-3 'such as dibutyryl adenosine-3', 5'-cyclic monophosphate sodium salt , 5'-
Cyclic monophosphate and its derivatives, guanosine-3 ', such as dibutyrylguanosine-3', 5'-cyclic monophosphate sodium salt,
5'-cyclic monophosphate and its derivative, γ-aminobutyric acid, retinoic acid, sodium alginate sulfate, magnesium ascorbate phosphate, vitamin A, vitamin A acid and its derivative, higher ascorbic acid fatty acid ester, and mammal With respect to whey and the like, synergistic effects can be obtained at the combined concentration similar to that of the whitening agent.

As described above, the external preparation of the present invention is a poultice, plaster, paste, cream, ointment, aerosol, emulsion, lotion, emulsion, essence, pack, gel, powder, foundation, sun care, bath. It is used in a known form used as a pharmaceutical such as salt, a quasi drug, and a cosmetic.

In the case of producing the external preparation of the present invention, various known active ingredients which are usually used, for example, carpronium chloride, cepharanthin, vitamin E, vitamin E nicotinate, nicotinic acid, nicotinic acid amide, benzyl nicotinate, and ginger. Peripheral vasodilators such as tincture, capsicum tincture, refreshing agents such as camphor and menthol, antibacterial agents such as hinokitiol, benzalkonium chloride and undecylenic acid, anti-inflammatory agents such as lysozyme chloride, glycyrrhizin and allantoin, cembly extract, garlic extract, Carrot extract, sardine extract, rosemary extract, aloe extract, loofah extract, ginkgo extract,
Various extracts derived from animals, plants and microorganisms such as elder extract, liver extract and lactic acid bacterium culture extract can be freely added and used. Further, the above-mentioned pharmaceuticals, quasi-drugs, cosmetics, other known active ingredients and surfactants, other base components such as oils and fats, known humectants, if necessary,
Thickener, antiseptic, antioxidant, UV absorber / scattering agent,
It goes without saying that various additives such as chelating agents, pH adjusters, fragrances and coloring agents can be used in combination.

Examples of moisturizers include polyhydric alcohols such as glycerin, propylene glycol, 1,3 butylene glycol, sorbitol, mannitol, polyethylene glycol and dipropylene glycol, amino acids,
NMF components such as sodium lactate, sodium pyrrolidonecarboxylate, hyaluronic acid, collagen, elastin,
Examples thereof include chondroitin sulfate, dermatan sulfate, fibronectin, ceramides, heparin-like substances, and water-soluble polymer substances such as chitosan.

Examples of the thickener include natural high molecular substances such as sodium alginate, xanthan gum, aluminum silicate, quince seed extract, tragacanth gum and starch, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, soluble starch and cationized cellulose. Examples thereof include semi-synthetic polymeric substances, carboxyvinyl polymers, polyvinyl alcohol, and other synthetic polymeric substances.

Examples of preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, 2,4,4'-trichloro-2'-.
Examples thereof include hydroxydiphenyl ether, 3,4,4'-trichlorocarbanilide, benzalkonium chloride, hinokitiol, resorcin, ethanol and the like.

Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, gallic propyl, and ascorbic acid.

Examples of the ultraviolet absorber include 4-methoxybenzophenone, octyldimethylparaaminobenzoate, ethylhexylparamethoxycinnamate, titanium oxide, kaolin, talc and the like.

Further, as the chelating agent, for example, ethylenediaminetetraacetate, pyrophosphate, hexametaphosphate, citrate, tartaric acid, gluconic acid and the like can be exemplified. As the pH adjuster, sodium hydroxide can be used. ,
Examples thereof include potassium hydrogen phosphate and the like.

[0040]

EXAMPLES Examples of the present invention and test examples for explaining the effects will be given below, but these do not limit the present invention at all.

< Production Example 1 > Capsicum water extract The dried capsicum was cut into small pieces, 5 L of water was added to 1 Kg of the cut pieces, and the mixture was stirred overnight at room temperature. After filtration, it was freeze-dried to obtain about 50 g of a freeze-dried product.

< Production Example 2 > Pepper ethanol extract Dried pepper was finely chopped, 5 kg of ethanol was added to 1 Kg of the chopped substance, and the mixture was stirred and extracted at room temperature overnight. After filtration, the ethanol extract was concentrated under reduced pressure to give an oily concentrate of about 1
50 g was obtained.

< Production Example 3 > Dill Dried dill was cut into fine pieces, 2.5 L of water and 2.5 L of ethanol were added to 1 Kg of the finely cut pieces, and the mixture was extracted at room temperature with stirring for 24 hours. After filtration, the aqueous ethanol extract is used as a synthetic adsorbent SP
It was passed through a column packed with -207 (manufactured by Mitsubishi Kasei Co., Ltd.), and the passing liquid was concentrated under reduced pressure to obtain about 100 g of a concentrate.

< Production Example 4 > Anise Dried anise was cut into small pieces, 2.5 L of water was added to 1 Kg of the small pieces, and the mixture was extracted at room temperature with stirring overnight. After filtration, 2.5 L of ethyl acetate was added to the water extract, and the mixture was stirred for 1 hr, the aqueous phase fraction was taken, 20 g of activated carbon was added thereto, and the mixture was stirred for about 1 hr. The filtrate was concentrated under reduced pressure to obtain about 80 g of a concentrate.

< Production Example 5 > Radix Notoginseng The dried Radix Notoginseng was cut into small pieces, 1 L of the cut pieces was added with 5 L of ethanol, and the mixture was extracted at room temperature with stirring overnight. After filtration,
Concentrate the ethanol extract under reduced pressure to about 200 g of concentrate.
Got

< Production Example 6 > Tarragon Dried tarragon leaves were finely chopped, and 1 kg of the chopped pieces was added with 10 L of water.
Was added, and the mixture was extracted at room temperature with stirring overnight. After filtration, 20 g of activated carbon was added to the ethanol extract and the mixture was stirred for about 1 hr.
After filtration, the filtrate was concentrated under reduced pressure to obtain about 200 g of a concentrate.

< Production Example 7 > Danshen The roots of dried Danshen are shredded, and 5L per 1kg of shredded material.
Ethanol was added, and the mixture was extracted at room temperature with stirring for 24 hours. After filtration, the ethanol extract was concentrated under reduced pressure to obtain about 8 concentrates.
0 g was obtained.

< Production Example 8 > Capsicum water extract-membrane treatment extract Dried capsicum was cut into small pieces, and 5 L per 1 kg of the cut pieces was cut.
Water was added, and the mixture was extracted at room temperature with stirring for 24 hours. The filtrate is treated with a reverse osmosis membrane (NTR-7410 manufactured by Nitto Electric Industry Co., Ltd.),
The permeate was freeze-dried to obtain about 17 g of a freeze-dried product.

< Test Example 1 > (Test for inhibiting melanin production by B16 cells) (Test method) 10% fetal bovine serum added so that the final concentrations of the plant extract and the skin-whitening agent of the present invention are shown in Tables 1 to 5. Culture B derived from mouse melanoma in Eagle MEM medium containing
After seeding 16 cells and culturing at 37 ° C. under 5% CO ₂ for 5 days, the cells were collected and suspended in PBS.
The cells were collected by centrifugation at 000 rpm for 5 minutes, and their black color was visually judged. The number of cells was measured with a blood cell counter after dispersing with trypsin.

As a control, a plant to which the above plant extract or skin lightening agent was not added was used. The criteria for judging the results are as follows. -: Similar to that without addition of the melanin production inhibitor (no addition) +: Shows a slightly lower degree of blackening than the non-addition group. ++: The degree of blackening is clearly smaller than that of no addition. +++: Shows a slight degree of blackening. ++++: White or gray, not black. ++++++: White

(Test Results) As shown in Tables 1 to 5, the capsicum extract, anise extract, dill extract, pearl oyster extract, and tarragon extract of the present invention were found to have an excellent melanin production-inhibiting effect, and were used in combination. It was found to synergistically enhance the effect of the agents.

[0052]

[0053]

[0054]

[0055]

[0056]

[0057]

[0058]

[0059]

[0060]

[0061]

< Test Example 2 > (Test for inhibiting melanin production by human melanoma cells) (Test method) 10% fetal bovine added so that the final concentrations of the plant extract and the skin-whitening agent of the present invention are shown in Tables 6 to 10. Human-derived melanoma cells (Ihara strain) were seeded in an Eagle MEM medium containing serum, cultured at 37 ° C. under 5% CO ₂ conditions for 5 days, then the cells were scraped, suspended in PBS, and 1,000 rpm × The cells were collected by centrifugation for 5 minutes, and their blackness was visually evaluated. The number of cells was measured with a blood cell counter after dispersing with trypsin. As a control, the one to which the above-mentioned plant extract was not added was used.

The criteria for judging the results are as follows. -: Similar to that without addition of the melanin production inhibitor (no addition) +: Shows a slightly lower degree of blackening than the non-addition group. ++: The degree of blackening is clearly smaller than that of no addition. +++: Shows a slight degree of blackening. ++++: White or gray, not black. ++++++: White

(Test Results) As shown in Tables 6 to 10, the capsicum extract, dill extract, anise extract, saliva extract, and tarragon extract of the present invention were found to have an excellent melanin production-inhibiting effect. It was found to synergistically enhance the effect of the agents.

[0065]

[0066]

[0067]

[0068]

[0069]

[0070]

[0071]

[0072]

[0073]

[0074]

< Test Example 3 > (Test for promoting collagen production by human fibroblasts) (Test method) Human-derived normal skin fibroblasts (CCD-2)
7SK is a plastic petri dish (Fa
lcon 3001), 0.8 × 10 ⁵ cells were seeded and cultured in an Eagle MEM medium containing 10% fetal bovine serum for 1 day, and then the medium was replaced with K-GM, and at the same time, the plant extract of the present invention and conventional collagen production were produced. The enhancer was added so that the final concentration in the medium was as shown in Table 11-1 to Table 11-7, and the cells were further cultured for 3 days. After completion of the culture, the culture supernatant was sampled, and the number of cells and the amount of procollagen in this culture solution were measured. For the measurement of procollagen, Pr
Using the ocollagen Type IC-peptide measurement kit (manufactured by Takara Shuzo Co., Ltd.), the sample-free group was used as a control, and the collagen amount ratio with the added group was expressed.

(Test Results) Tables 11-1 to 11-6
As shown in, the chili pepper extract, dill extract, anise extract, pearl oyster extract, and tarragon extract of the present invention have excellent cell proliferation effects and collagen production-enhancing effects, and are conventionally known in combination therewith. It was observed that the effect of the collagen production-promoting agent is enhanced synergistically. Also, as shown in Table 11-7,
A particularly high cell proliferation effect was observed in the pepper extract-membrane treated extract. In the table, the control ratio (A) represents the cell growth control ratio, and the control (B) represents the collagen amount control ratio per cell.

[0077]

[Table 0001]

[0078]

[0002]

[0079]

[Table 0003]

[0080]

[0004]

[0081]

[Table 0005]

[0082]

[Table 0006]

[0083]

The following is a formulation example of the external preparation for skin containing the plant extract of the present invention. This formulation example is for showing a suitable formulation of the plant extract of the present invention. It will be appreciated that the scope of the invention is not limited. In addition, in the formulation examples, the “appropriate amount” means an amount that makes 100% by weight as a whole.

<Formulation Example 1> Cream (% by weight) A Monostearic acid Polyethylene glycol (40 EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Liquid paraffin 10.0 Glyceryl trioctanoate 10.0 Kojic acid 1.0 Dill extract 0.1 B Glycerin 5.0 Ethylparaben 0.1 Purified water Appropriate amount A component belonging to A is dissolved by heating. Separately, the components belonging to B are melted by heating. After adding B to A, stirring and emulsifying, cooling was performed to produce a cream.

<Formulation Example 2> Cream (% by weight) A Monostearate Polyethylene glycol (40 EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Liquid paraffin 10.0 Glyceryl trioctanoate 10.0 B Glycerin 5.0 Ethylparaben 0.1 Pepper water extract-membrane treatment extract 0.75 Purified water Appropriate amount A and B are heated and dissolved at 80 ° C., and B is added to A and stirred. Cooled to produce cream.

<Formulation Example 3> Cream (% by weight) A Monostearate Polyethylene glycol (40 EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Liquid paraffin 10.0 Glyceryl trioctanoate 10.0 B Glycerin 5.0 Ethanol 0.2 Capsicum ethanol extract 0.1 Capsicum water extract 0.5 Ethylparaben 0.1 Purified water Appropriate amount A and B are heated and dissolved at 80 ° C., respectively. B was added to A, stirred and cooled to produce a cream.

<Formulation Example 4> Cream (% by weight) A Monostearic acid Polyethylene glycol (40 EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Liquid paraffin 10.0 Glyceryl trioctanoate 10.0 B Glycerin 5.0 Magnesium ascorbyl phosphate 0.05 Dibutyl-3′5′-adenosine Cyclic monophosphate sodium salt 0.05 Danshen extract 0.1 Ethanol 0.2 Pepper water extract 0 .5 Kojic acid 0.1 Ethylparaben 0.1 Purified water Appropriate amount A and B were dissolved by heating at 80 ° C., B was added to A, stirred and cooled to produce a cream.

<Formulation Example 5> Emulsion (wt%) A Polyoxyethylene sorbitan monostearate (20 EO) 1.0 Polyoxyethylene monostearate sorbit (60 EO) 0.5 New oily glyceryl monostearate 1. 0 Stearic acid 0.5 Behenyl alcohol 0.5 Avocado oil 4.0 Glyceryl trioctanoate 4.0 B Pepper water extract 2.0 1,3-Butylene glycol 5.0 Methylparaben 0.2 Purified water Appropriate amount of ingredients belonging to A Dissolve by heating. Separately, the components belonging to B are melted by heating. After adding B to A, stirring and emulsifying, it was cooled to produce an emulsion.

<Formulation Example 6> Lotion (% by weight) Polyoxyethylene hydrogenated castor oil (60 EO) 8.0 Ethanol 15.0 Ethylparaben 0.1 Citric acid 0.1 Sodium citrate 0.3 1,3 -Butylene glycol 4.0 Disodium edetate 0.01 Kojic acid glucoside 0.5 Tarragon extract 0.05 Purified water Appropriate amount The above components were mixed, uniformly stirred and dissolved to produce a lotion.

<Formulation Example 7> Cream pack (% by weight) A Veegum 5.0 Squalane 2.0 Propylene glycol 5.0 Vitamin B ₁₂ 0.05 Kojic acid glucoside 1.0 Nukuju extract 1.0 Purified water suitable Quantity B Zinc oxide 10.0 C Ethanol 5.0 Ingredients belonging to A are mixed and stirred to swell, and B is added little by little. C was gradually added to this and kneaded until a paste was formed to produce a cream pack.

<Formulation Example 8> (wt%) A Veam gum 5.0 Squalane 2.0 Propylene glycol 5.0 Vitamin B ₁₂ 0.05 Piscine extract 0.5 γ-Aminobutyric acid 0.05 Purified water Appropriate amount B zinc oxide 10.0 C ethanol 5.0 Danshen extract 0.5 retinoic acid 0.05 A components belonging to A are mixed and stirred to swell, and B is added little by little. Further, C was gradually added and kneaded until a paste was formed to produce a cream pack.

<Formulation Example 9> Essence (wt%) 1% carboxyvinyl polymer solution 10.0 Glycerin 20.0 Hyaluronic acid 0.5 Ethanol 1.0 Kojic acid galactoside 3.0 Pepper water extract 5.0 Purified water suitable Amount The above components were mixed, uniformly stirred and dissolved to produce an essence.

<Formulation Example 10> Hydrophilic ointment (% by weight) A Polyoxyethylene cetyl ether 2.0 Glyceryl monostearate 10.0 Liquid paraffin 10.0 Vaseline 4.0 Cetanol 5.0 Liquiritin 0.5 Anise extract 10.0 B Propylene glycol 10.0 Methylparaben 0.1 Purified water Appropriate amount Components of A are heated and dissolved. Separately, the components belonging to B are dissolved by heating. After adding B to A, stirring and emulsifying, it was cooled to produce a hydrophilic ointment.

[0095]

The external preparation for skin containing the specific plant extract as an active ingredient provided by the present invention is highly safe and has an excellent inhibitory effect on melanin production. Therefore, it is an agent for preventing spots and freckles and treating pigmentation disease. As it is also excellent in collagen production promoting action and cell growth promoting action, it is useful as a wrinkle preventive agent and as a skin anti-aging agent.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Office reference number FI Technical display location A61K 35/78 N 7167-4C S 7167-4C T 7167-4C C 7167-4C 37/02 8314- 4C

Claims (5)

[Claims] 1. An external preparation for skin, which comprises, as an active ingredient, at least one plant extract selected from the group consisting of capsicum, dill, anise, pearl oyster, and tarragon. 2. The external preparation for skin according to claim 1, further comprising at least one kind of whitening agent. 3. A whitening agent is kojic acid, ascorbic acid,
Hydroquinone, liquiritin or their derivatives,
The external preparation for skin according to claim 2, which is at least one selected from the group consisting of and extract of placenta. 4. The external preparation for skin according to claim 1, further comprising at least one collagen production enhancer. 5. The agent for promoting collagen production is Danshen extract,
Ascorbic acid magnesium phosphate, adenosine-
At least one selected from the group consisting of 3 ′, 5′-cyclic monophosphate and its derivative, guanosine-3 ′, 5′-cyclic monophosphate and its derivative, γ-aminobutyric acid and retinoic acid. The external preparation for skin according to claim 4.