JPH06233671A - Culture container - Google Patents

Abstract

PURPOSE:To obtain a culture container capable of performing pipet operation or scraping operation without impairing the airtightness as the container and causing a dead angle in operation after the culturing and surely and readily recovering a cell, etc. CONSTITUTION:This culture container is composed of a body part 1 composed of a bottom wall 3 and sidewalls 4, surrounding the bottom wall and having a neck part 5 to be an opening therein and a lid part 2 engaged with the tops of the sidewalls parallel to the bottom wall. The engaging part of the body part with the lid part is detachably and temporarily bonded.

Description

Detailed Description of the Invention

[0001]

The present invention relates to bacterial culture, cell culture,
The present invention relates to a plastic, disposable culture container used for tissue culture and the like.

[0002]

2. Description of the Related Art A culture container targeted by the present invention is generally called a culture flask and is widely used in the fields of bacterial culture, cell culture, tissue culture and the like. In particular, in the field of cell culture, it is widely used along with culture dishes and culture plates. The reason why this culture flask is used is
By putting a cap on the neck, it becomes almost sealed,
It is easy to handle during culturing, there is no risk of spilling the medium when carrying it like a dish for culturing, and there is little risk of invasion of foreign bacteria from the outside.

However, when the culture flask is used, a cell suspension is injected through the neck, which is an opening, a medium is injected or exchanged with a pipette, and the cultured cells are peeled off with a scraping jig. The cells are sucked into a cell suspension with a pipette, and collected. Among these operations, there are no particular problems in operations from outside to inside the culture container, such as injection of cell suspension or medium, but from inside to outside of the culture container, that is, pipetting at the time of collecting cells or There is a drawback that a blind spot is generated in the operation of the scraping jig, and the tool cannot reach the corners of the culture container. Further, since the operation is performed through a narrow neck, there is a problem that the working efficiency is low and a considerably long time is required when cells are collected from many culture vessels.

In order to make up for such drawbacks, even if a design is made such that the diameter of the neck opening is as large as possible, when the containers are stacked and put in the incubator, more space is required due to the limited space. Since it is necessary to accommodate the culture container, the height of the culture container body is limited, and therefore the diameter of the opening is also limited and cannot be increased.

As one of means for solving these drawbacks, a method of increasing the diameter of the open end of the neck and narrowing the upper and lower parts toward the attachment part of the neck so that the height of the side wall does not become high. (Japanese Utility Model Laid-Open No. 63-63400, US Pat. No. 4,770,854), but when the diameter of the open end is increased, the necks interfere with each other when the containers are capped and stacked in order. As a result, the design of the cap structure becomes difficult and airtightness may be impaired. Therefore, there is a restriction that the opening end cannot be made larger than a certain value.

[0006] Therefore, in order to facilitate the recovery of cells and the like after the completion of the culture, a plastic sheet is welded to the lid on the upper surface of the flask, and after the culture, the sheet is peeled or cut off so that it does not pass through the neck. There is also a device devised to collect cells and the like. However, when the sheet is stuck as the lid in this way, the sheet is weak in strength, so that it tends to become unstable when a plurality of flasks are stacked, and is not suitable for stacking. Also, when cutting the sheet with a cutter, etc.
If care is not taken such as sterilizing the blade of the cutter, there is also a problem that bacteria etc. are mixed into the flask from the outside.

[0007]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the present situation of culture flasks, and its purpose is to recover cells and the like without impairing airtightness. It is to provide a culture container in which a pipette and a scraping jig can be easily operated.

[0008]

Means for Solving the Problems That is, the present invention comprises a bottom wall which is a culture surface and a side wall which surrounds the bottom wall, and a main body having a neck which is an opening in the side wall, and a side wall which is parallel to the bottom wall. A culture container, which is a plastic molded product composed of a lid portion fitted to the upper end portion of the side wall, wherein the fitting portions of both are temporarily adhered so as to be removable.

Hereinafter, the culture container according to the present invention will be described in detail with reference to the drawings. FIG. 1 is a view showing a culture container according to an embodiment of the present invention, (a) is a perspective view showing the whole,
(B) is a side sectional view.

The culture vessel in the present invention is a plastic culture flask used for cell culture, tissue culture, bacterial culture, etc., and has a bottom wall (3) as a culture surface and a side wall (4) surrounding it. And a side wall (4) having a container body (1) provided with a neck (5) serving as an opening for injecting a sample solution or a medium at the time of use, and the side wall (4).
Removably fitted to the upper end of the parallel to the bottom wall (3),
It is composed of a lid part (2) to be attached.

As shown in FIG. 2, the fitting portion between the main body portion (1) and the lid portion (2) is notched on the entire circumference or a part of the upper end portion of the side wall (4) of the main body portion (1). (6) or the groove (7) is provided, and the convex portion (8) is provided at the position corresponding to the notch (6) or the groove (7) on the lower surface of the peripheral portion of the lid portion (2). Has been formed. On the contrary, by providing a groove (9) in the whole or a part of the lower surface of the peripheral edge of the lid (2), the upper end of the side wall (4) of the main body (1),
Alternatively, a method of fitting a convex portion (10) provided at a position corresponding to the groove (9) on the upper end may be adopted. This not only allows for smooth alignment when assembling the main body part (1) and the lid part (2), but also increases the area of the fitting part and ensures the sealing and adhesiveness of the fitting part. The effect is obtained.

The body portion (1) and the lid portion (2) are fitted and assembled by temporary adhesion with silicone rubber. After the liquid silicone rubber is applied or filled in the notches (6) or the grooves (7, 9) of the fitting portion, the silicone rubber is cured by combining the body portion (1) and the lid portion (2). The silicone rubber layer (11) as shown in FIGS. 2 and 3 is formed to serve as a sealing adhesive layer. In general, silicone rubber does not completely adhere to hydrophobic plastics, so that the lid (2) can be easily removed from the main body (1) even after the adhesion. In addition, the length of the lid (2) and /
Alternatively, the width is made slightly larger than that of the main body (1), and along at least one of the side walls surrounding the bottom wall (3),
A protrusion with a width of about 0.3 to 1.5 mm as shown in FIG. 3 (12)
If it is provided, it is convenient to remove the lid portion (2) from the main body portion (1).

If both the main body part (1) and the fitting part of the lid part (2) are hydrophobic, the silicone rubber layer (11) will remain on either the main body part side or the lid part side when the lid part is removed. It remains uncertain whether or not it will remain. Therefore, by applying a hydrophilic treatment to either the main body portion (1) or the lid portion (2), the silicone rubber layer (11) is surely subjected to the hydrophilic treatment treatment when the lid portion (2) is removed. You can leave it on the side you did. As a result, even if the lid part (2) is once removed, the remaining silicone rubber layer (11) serves as a packing, and the lid part (2) is fitted again to the main body (1) for reuse or culture. Can also be continued. Since it is highly necessary to apply hydrophilic treatment to the bottom wall (3) that is the culture surface for the purpose of imparting cell adhesiveness, if the main body (1) side is subjected to hydrophilic treatment, it will be hydrophilized once. Processing is advantageous in terms of processing.

The plastic forming the culture flask in the present invention must be transparent.
It is preferable to use polystyrene, polyester, polycarbonate or the like. The cap need not be transparent and may be the plastic normally used as a cap. or,
Silicone rubber is suitable as an adhesive used for sealing and adhering the fitting portion, since it is required to have no cytotoxicity and maintain sealing property. As the silicone rubber, it is preferable to use a silicone rubber that is liquid before curing and does not require high temperature for curing, and is preferably room temperature curable, from the viewpoint of being applied to the thermoplastic resin.

[0015]

EFFECTS OF THE INVENTION According to the present invention, the pipette and scraping can be repaired without passing through the neck by removing the lid when recovering cells after culturing without impairing the airtightness of the culture container during culturing. Since the operation can be performed by the tool, it is possible to provide a culture container in which there is no blind spot in the operation of the pipette or the scraping jig and the cells and the like can be collected reliably and easily.

[Brief description of drawings]

1A and 1B are views showing an embodiment of a culture container according to the present invention, in which FIG. 1A is a perspective view and FIG. 1B is a side sectional view.

FIG. 2 is a diagram showing an example of a fitting structure of a main body and a lid.

FIG. 3 is a view showing another embodiment of the fitting structure of the main body and the lid.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Main body part 2 Lid part 3 Bottom wall 4 Side wall 6 Notch 7, 9 Groove 8, 10 Convex part 11 Silicone rubber layer 12 Protrusion

Claims (7)

[Claims] 1. A main body having a bottom wall serving as a culture surface and a side wall surrounding the bottom wall, the main body having a neck serving as an opening in the side wall, and a lid fitted to the upper end of the side wall parallel to the bottom wall. A culture container characterized in that it is a plastic molded article composed of a part and a fitting part of both parts is temporarily adhered so as to be removable. 2. The fitting portion between the main body portion and the side wall includes a notch or a groove provided on the entire circumference or a part of the upper end portion of the side wall, and a convex portion provided on the whole or a part of the lower peripheral surface of the lid portion. The culture container according to claim 1, wherein the culture container is formed. 3. The fitting portion between the main body portion and the side wall is fitted with the convex portion provided at the upper end portion or the upper end portion of the side wall of the main body portion with respect to the groove provided on the whole or a part of the lower surface of the peripheral edge of the lid portion. The culture container according to claim 1, wherein the culture container is formed as follows. 4. The culture according to any one of claims 1 to 3, wherein the peripheral edge of the lid portion forms a protrusion along at least one of the side walls surrounding the bottom wall. container. 5. The culture container according to claim 1, wherein the adhesive for temporarily adhering the fitting portion between the main body portion and the lid portion is liquid silicone rubber. 6. The culture container according to claim 5, wherein one of the side wall upper end fitting portion of the main body portion and the peripheral edge lower surface fitting portion of the lid portion is subjected to a hydrophilic treatment. 7. The culture container according to claim 5, wherein the inner surface of the main body portion and the side wall upper end fitting portion are subjected to a hydrophilic treatment.