JPS5894383A - Cover for instrument for cultivation - Google Patents

Abstract

PURPOSE:To enable the airtight sealing of the tops of the dents of a main body when the main body is fixed to a cover, by providing the inner face of the cover for overspreading the main body having the dents for cultivating anaerobically a microorganism or cell with an elastic material at a position where the innerface is brought into contact with the surroundings of the tops of the dents. CONSTITUTION:The inner face of the cover 1 for over spreading the main body 5 having the dents 6 for cultivating anaerobically a microorganism or cell is provided with the elastic materials 25 at positions where the inner face is brought into contact with the surroundings of the tops of the dents 6. Preferably the shape of the eleatic materials 25 is made into a shape corresponding to the surroundings of the tops of the dents 6, a laminar shape a little larger than the surroundings of the tops or a ring-shaped form. Natural rubber, silicone rubber, etc. is used as a material for the elastic material 25, the thickness of the elastic materials is about 0.5-2mm., and they are attached to the inner face of the cover 1 by an adhesive, etc. Consequently, each of the top of the dents 6 is securely sealed airtightly by the elastic materials 25 when the main body 5 is overspread with the cover 1.

Description

Detailed Description of the Invention (1) Background of the Invention Technical Field The present invention relates to a lid for a culture device. More specifically, the present invention relates to a lid of a culture device for sealing or anaerobically culturing microorganisms or cells.

Prior art and its problems There are two types of microorganism or cell culture: culture carried out using open strings, and culture using sealed strings as anaerobic culture.

Conventionally, when culturing in a sealed system, the main body of a Nyare with culture recesses or a multi-hole plate with multiple culture recesses is covered with an adherent, adhesive tape is applied to the outer periphery of the side surface, and the gap is filled with adhesive. It is done by sealing it with tape to make it airtight, or by putting the paycheck in another sealable container to make it airtight.

However, in the former case, if the culture medium stored in the recess is liquid, the culture medium will spill out unless the main body is held horizontally, making it difficult to apply adhesive tape to the outer periphery of the side surface, which requires a skilled worker. There are drawbacks. Also, in cases like the latter.

Lack of ease of work.

On the other hand, especially when using a multi-hole plate as the main body, an adhesive sheet is directly adhered onto the culture recesses or culture holes called wells to seal the top of each well.

However, in this case, since the adhesive sheet is located above the well and the adhesive faces the culture medium, there is a risk that the culture medium may be affected by the adhesive.

Furthermore, if the top surface of the well wall is wet or the width of the top surface is narrow, it is difficult to completely seal the well, and since culture is performed under high humidity conditions, parts of the adhesive sheet may peel off during culture.

■Object of the Invention The present invention was made in view of the above-mentioned circumstances, and its main object is to provide a lid for a culture device that eliminates the above-mentioned drawbacks and inconveniences. More specifically, it is an object of the present invention to provide a lid for a culture device that has a simple structure and can be sealed extremely easily and easily.

The inventor of the present invention has repeatedly studied this object, and as a result, has completed the present invention.

That is, the present invention provides a lid covering a main body having at least one recess for cultivating microorganisms or cells, and an elastic body is provided at a position of the lid in contact with the upper periphery of the recess of the main body, The lid of the culture device is characterized in that the lid of the culture device is configured such that the upper part of the recess can be sealed when the main body and the mass are fixed.

Embodiments of the present invention relate to specific structures that more effectively achieve the above objects, and are as follows.

i) The lid of the culture device of the present invention, in which the elastic body is plate-shaped and has a shape and size corresponding to the upper periphery of the recess of the main body.

ii) The lid of the culture gE recommendation tool of the present invention, wherein the elastic body is in the shape of a ring corresponding to the upper periphery of the recess of the main body.

(2) Specific Structure of the Invention The present invention will be explained in detail below with reference to the embodiments shown in FIGS. 1 to 3 and FIG. AG4.

As shown in FIGS. 3 and 4, the lid 1 of the culture device of the present invention encloses a main body 5 having at least one recess 6 for culturing microorganisms or cells.

In this case, the main body 5 may be in the shape of a petri dish having four parts for culturing, or as shown in the figure, it may be in the shape of a multi-hole plate having a large number of recesses 6, ie, wells, for culturing. The structure, shape, material, etc. can be varied.

An elastic body is provided on the inner surface 15 of the lid 10 that comes into contact with the upper periphery of each recess of the main body 5.

There are various ways to provide the elastic body on the inner surface 15 of the lid 1, and a plate-shaped elastic body may be provided over almost the entire inner surface. As shown in FIG. 4, a plate-shaped elastic body 25 preferably has a shape corresponding to the upper periphery of the recess 6 of the main body 5, and more preferably a size slightly larger than the upper periphery of the recess 6. It is preferable to provide one corresponding to each recess 6. At this time,
When the lid 1 is placed over the main body 5, the upper part of each of the corresponding four parts 6 is sealed by a plate-shaped elastic body 25, as shown in FIG.

Further, as shown in FIGS. 1 to 3, the first elastic body is preferably a ring-shaped elastic body 21 having a shape corresponding to the upper peripheral edge of the corresponding recess 6, and the outer edge thereof is set to the upper part of the corresponding recess 6. A more favorable result can be obtained if it is made larger than the circumferential edge and its inner edge is made slightly smaller than the upper circumferential edge of the corresponding recess 6.

At this time, the sealing of each recess 6 is ensured, and since the lid 1 is made transparent, money can be placed inside the recess 6 from the outside. In such a case, by covering the main body 5 with the lid 1, each corresponding recess 6 is sealed by the ring-shaped elastic body 21 and the inner surface 15 of the lid.

Note that this preferably plate-shaped, ring-shaped, etc. elastic body can be made of natural or synthetic rubber, silicone rubber, etc., and among these, silicone rubber is most suitable.

Further, the thickness thereof may be approximately 0.5 to 2 m.

In order to provide such an elastic body on the 21st surface of the lid body 1, it is possible to attach it with an adhesive or the like, or to use the fi lid body itself as a pillar body and attach a ring-shaped or plate-shaped elastic body 21, 25 to the lid body. It may be formed integrally with the body 1.

As shown in FIGS. 3 and 4, such a lid body 1 is placed over the main body 5 and fixed together, and the upper part of each recess 6 is sealed with an elastic body.

In this case, in order to fix the lid l to the main body 5, for example, an elastic band may be hung or passed.

However, in a more preferred embodiment, as shown in FIG. It is preferable to fit it into the main body 5. This is because no separate fixing device is required at this time.

In such a case, the elastic material 7 may be provided on at least one of the outer side surface of the main body 5 and the inner side surface of the lid 1, and the shape, arrangement, etc. of the elastic material can be changed in various ways. .

However, as shown in FIG. 3, if the elastic material 7 is a ring and a second ring is provided around the outer surface of the 50 parts of the main body or the inner surface of the side part of the lid body 1, the sealing becomes more reliable.
If the ring-shaped elastic material 7 is used in a groove formed around the outer side surface of the main body 5 or the inner side surface of the intangible body 1 as shown in the figure, manufacturing becomes easy.

Note that such an elastic material may also be made of the same material as described above.

■ Specific effects of the invention The lid 1 of the culture device of the present invention stores a culture medium in the recess 6 and is used by covering the main body 5 containing the living organism or cells and fixing and sealing both parts. .

At this time, each corresponding recess 6 is sealed by the elastic body 21, 25, and the sealing is performed reliably and well. In this case, like when using an adhesive sheet,
The seal will not be broken during culturing. In addition, since the upper part of the recess 6 is sealed, when fixing the lid l and the main body 6,
Even if you use this, the medium will not spill or mix with other media.

Furthermore, unlike when using adhesive tape, there is no adverse effect on culture.

Such an effect can be achieved by moving the elastic body 21.25 to the lid body 1.
This can be achieved with an extremely simple configuration, provided on the inner surface of the

In this case, if the elastic body is formed into a plate shape corresponding to the upper peripheral edge of the recess 6 according to the embodiment i), the reliability of sealing will be improved.

Further, according to the above embodiment il), if it is formed into a ring shape,
The reliability of sealing is improved, and observation during culturing becomes easier.

[Brief explanation of drawings]

Figures 1 to 3 are diagrams for explaining the present invention in detail, of which Figure 1 is a perspective view, Figure 2 is a sectional view, and Figure 3 is a sectional view.
The figure is a sectional view showing the assembled state with the main body. Fourth
The figure is a sectional view showing another embodiment of the present invention assembled with the main body. 1・・・・・・・・・・・・・・・Lid body 15・・・・・・
......Inner surface 21...Ring-shaped elastic body 25...
・]・・・・・・Plate-like elastic body 5・・・・・・・・・
...Body. 6・・・・・・・・・・・・・・・Concave portion patent applicant
Terumo Co., Ltd. Representative Patent Attorney Akira Ishii - Sai 2 Zuga 3 Zuga 4

Claims (1)

[Claims] 1. At least one recess for culturing microorganisms or cells.
a body for covering a main body having an elastic body, the elastic body being provided at a position of the lid body in contact with an upper periphery of the recess of the body;
An intangible culture device characterized in that the upper part of the recess can be sealed when the main body and the attaching body are fixed. 2. The lid of the culture device according to claim 1, wherein the elastic body is plate-shaped and has a shape and dimensions that correspond to the upper periphery of the recess of the main body. 3. The lid of the culture device according to claim 1, wherein the elastic body has a ring shape corresponding to the upper periphery of the recess of the main body.