JP2010022917A - Reaction vessel, reaction device and method of blocking reaction vessel - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a reaction vessel capable of securely blocking a reaction chamber. <P>SOLUTION: The reaction vessel includes a body 2 and a lid 3 which can block each other, and concave-shaped wells 6 for placing a reaction reagent, are formed in the body 2. In addition, an introduction inlet 7 and an air vent 8 are formed on both sides of each of the three wells 6 aligned at specified intervals, and are allowed to communicate with the outside. Further, a flow path 9 which communicates with each well 6 is formed starting with the introduction inlet 7 to make the flow path 9 connect with the air vent 8, and the reaction reagent to be introduced from the introduction inlet 7, is supplied to each well 6 through the flow path 9. A concave-shaped well 6' is also formed in the lid 3 in a position opposed to the well 6. Besides, an elastic sheet 11 is interposed between the body 2 and the lid 3, and a hole 12 is formed in a position corresponding to the well 6 in the elastic sheet 11. The reaction vessel is inserted into between a base of a loading member and a pressing member, and then, is securely fixed by an immobilizing member in a pressurized state. The elastic sheet 11 of the reaction vessel becomes changed elastically to seal the flow path 9 and, consequently the wells 6 and 6' are independently sealed in a liquid-tight condition. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a reaction vessel, a reaction apparatus, and a method for closing a reaction vessel that can liquid-tightly close a reaction vessel including, for example, a gene analysis chip.

In recent years, as a method of performing a biological reaction in a laboratory without using a large laboratory apparatus or a large amount of reaction reagent, it has been generally performed to use a minute reaction system such as a microchip or a lab-on-chip. Yes.
As such a small biological reaction instrument, a reaction container having a large number of minute wells such as a microtiter plate is used. In biological reactions, a plurality of reagents or different reagents are usually applied to the same sample and analyzed at the same time. In such a case, different reagents are placed in a plurality of wells in the reaction container, and the sample is introduced into the wells through the flow path. Then, before the reaction starts, the wells of the reaction vessel are made independent by closing the flow path so that a desired reaction is performed for each well.
An example of such a reaction vessel is described in Patent Document 1 below. In the reaction vessel described in Patent Document 1, after introducing a sample into an assembly of wells having a deformable seal, each well is made independent by closing the flow path by permanently deforming the seal using a jig. Thus, a sample processing apparatus for causing a desired reaction for each well is disclosed.
JP-T-2004-502164

  However, in the sample processing apparatus described in Patent Document 1, the operator permanently deforms the seal manually using a jig so as to seal the flow path. There was a problem that the sample could not be completely sealed and the sample flowed from one well into the other surrounding wells to cause contamination and the desired reaction could not be performed.

  The present invention has been made in view of such circumstances, and an object of the present invention is to provide a reaction vessel, a reaction apparatus, and a method for closing the reaction vessel that can more reliably close the reaction chamber.

A reaction container according to the present invention has a main body and a lid that can be closed with each other, and has a reaction chamber in which a reaction reagent is placed in at least one of the main body and the lid, and is interposed between the main body and the lid. It is characterized by interposing an elastic body.
According to the present invention, the reaction body can be sealed in a liquid-tight manner by closing the main body and the lid and pressing the elastic body to elastically deform the elastic body, so that the sample flows into the other reaction chamber. Thus, it is possible to reliably prevent the occurrence of contamination and perform a desired reaction.
The reaction chamber may be a well having a hollow shape, or may be a surface such as a flat surface on which a reagent or the like is simply placed. Further, the reaction chamber may be provided with a flow path and communicated, or may be configured such that the flow path is not communicated.

The elastic body is preferably disposed so as to surround the reaction chamber.
By elastically deforming the elastic body by pressing the main body and the lid, the periphery of the reaction chamber opening can be reliably closed.
In addition, the elastic body may be hydrophobic and can prevent the absorption of moisture contained in the sample or the like to suppress the deterioration of the elastic properties of the elastic body.
In addition, a reaction reagent channel may be connected to the reaction chamber.
Even in this case, the elastic body is elastically deformed by closing the main body and the lid, whereby the flow path can be liquid-tightly closed and the separation independence of the reaction chamber can be ensured.
Further, at least one of the main body and the lid may be provided with an inlet for the reaction reagent and / or an air port for venting air. The reaction reagent can be introduced from the introduction port, and the air remaining inside can be discharged from the air port.

The reaction apparatus according to the present invention includes a heating / cooling means for heating or cooling any of the reaction vessels described above.
By heating the reaction vessel from the outside using the heating / cooling means, the conformity between the elastically deformed elastic body and the main body and the lid on both sides can be improved, and the adhesion can be further improved. Further, the reaction in the reaction chamber can be promoted by heating or cooling the reaction vessel with the heating / cooling means.

The method for closing a reaction container according to the present invention has a reaction chamber in which a reaction reagent is placed in at least one of a main body and a lid, and the elastic body is elastically deformed by closing the main body and the lid with an elastic body interposed therebetween. Thus, the reaction chamber is sealed in a liquid-tight manner.
According to the present invention, by closing the main body and the lid and pressing the elastic body, the elastic body can be elastically deformed to seal the reaction chamber in a liquid-tight manner, and a sample or the like flows into the other reaction chamber to cause contamination. It is possible to reliably prevent the occurrence of the desired reaction.

In addition, a flow path communicating with the reaction chamber may be formed in at least one of the main body and the lid, and the flow path may be liquid-tightly sealed by elastically deforming the elastic body.
The independence of the reaction chamber can be secured by elastically deforming the elastic body and closing the flow path in a liquid-tight manner.
In addition, the closed reaction vessel may be heated or cooled, and the adhesion between the elastic body elastically deformed by heating the reaction vessel from the outside and the body and lid on both sides is improved. The reaction in the reaction chamber can be promoted by heating or cooling the reaction vessel.

  According to the reaction vessel, the reaction apparatus, and the method for closing the reaction vessel according to the present invention, when the lid and the main body are closed, the elastic body interposed between the two is elastically deformed to close the reaction chamber in a liquid-tight manner. As compared with a conventional reaction vessel, the reaction chamber can be more reliably closed and sealed without permanent deformation of the seal with a jig or the like.

Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings.
First, the reaction container according to the first embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a plan view and a side view of the reaction vessel, FIG. 2 is an explanatory view showing a state in which the reaction vessel of FIG. 1 is opened, and FIG. 3 is a partial sectional view taken along line AA in FIG. FIG. 4 is a perspective view of a state in which the reaction container is placed on the weighting member, and FIG. 5 is a perspective view of a pressing and fixing state in which the reaction container is fixed by applying a load to the reaction container. FIG.
A reaction vessel 1 shown in FIG. 1 is composed of, for example, a substantially rectangular plate-shaped main body 2 and a lid 3 which are laminated with each other, and an elastic sheet 11 as an elastic body sandwiched between the main body 2 and the lid 3. Has been. The connecting portion of the main body 2 and the lid body 3 forms a hinge portion 4 so that the main body 2 and the lid body 3 can be opened and closed with their inner surfaces facing inside. The main body 2 and the lid 3 may be separated from each other without being connected.
In the reaction vessel 1, the main body 2 and the lid 3 are names for convenience, and the configurations or names of the main body 2 and the lid 3 may be interchanged with each other.

Next, each structure of the reaction vessel 1 will be described in detail with reference to FIGS. The main body 2 is provided with a plurality of wells 6 as reaction chambers on the inner surface thereof. For example, as shown in FIG. 2, three wells 6 are arranged in two rows at predetermined intervals. Each well 6 is formed, for example, as a substantially hemispherical or inverted frustoconical recess or depression, in which a reaction reagent is placed. Such a well 6 may have a volume of, for example, about 100 μl (microliter), more preferably a volume of 5 to 60 μl (microliter).
On both sides of the wells 6 in each row, for example, an introduction port 7 and an air port 8 penetrating the back surface of the main body 2 are formed. In each row of wells 6, a groove-shaped channel 9 extends from the introduction port 7 to each well 6 and the air port 8. For example, a micropump (not shown), a manual liquid dispenser (Pipetteman (R)), an automatic liquid dispenser or the like is inserted into the introduction port 7 and introduced by this liquid feeder. A reaction reagent is introduced into the flow path 9 and each well 6 from the mouth 7. The two flow passages 9 and 9 are arranged substantially in parallel.
The air port 8 can discharge the air in the well 6 and the flow path 9 to the outside.
In the example shown in FIG. 2, the introduction port 7 and the air port 8 are formed at both ends of the flow path 9 for each row of the wells 6, but the two flow paths 9 are merged at both ends. One introduction port 7 and one air port 8 may be provided and connected.

In addition, about the material of the main body 2, it can create from the transparent, opaque appropriate general material as needed. For example, in the case of an opaque material, fluororesins such as Teflon (registered trademark), polypropylene (PP), polyurethane, various resins such as bakelite and urea resin, aluminum, silver, copper and various alloys can be used.
Moreover, when using a transparent material, various resin materials, such as inorganic materials, such as glass and a zircon, polycarbonate, a polypropylene, PET (polyethylene terephthalate), can be utilized.
In any case, the sample or reagent used in the reaction vessel 1 or a material that does not adversely affect the reaction can be selected as appropriate.
The main body 2 can be subjected to coating, surface modification, surface processing, and the like necessary for reducing reaction inhibition and workability during assembly. For example, coating of a coating agent for imparting hydrophilicity or water repellency or formation of a coating layer (such as application of a coating film or surface treatment), formation of an adhesive or a welding layer (sandwiching of the layer) , Bonding, etc.).
The introduction port 7 and the air port 8 may be configured to open on the side surface instead of the configuration opening on the back surface of the main body 2.

Further, the lid 3 is provided with a plurality of wells 6 'as reaction chambers on the inner surface thereof, and the wells 6' are arranged in two rows at predetermined intervals, for example, as shown in FIG. Each well 6 'of the lid 3 is disposed so as to face each well 6 of the main body 2 when the main body 2 and the lid 3 are closed and closed by the hinge portion 4 (FIG. 3). reference). The configuration of the lid 3 is almost the same as that of the main body 2 and the material thereof is also the same.
In the lid body 3 shown in the figure, the flow path 9, the introduction port 7, and the air port 8 provided in the main body 2 are not provided, but these may be provided, and in that case, the line 3 is substantially line symmetric with respect to the hinge portion 4. You may form in.
In addition, it is good also as what uses a different material about the main body 2 and the cover body 3, and may use the same material. In order to improve the thermal conductivity, one can be made of metal, or the transparent main body 2 and / or lid 3 can be used to measure the reaction with electromagnetic waves such as fluorescence.

The elastic sheet 11 has, for example, a substantially rectangular sheet shape and is formed smaller than the dimensions of the main body 2 and the lid body 3. The elastic sheet 11 is formed with a hole 12 so as to face the well 6 of the main body 2 when the elastic sheet 11 is mounted and closed between the main body 2 and the lid 3. Each hole 12 is preferably positioned so as to face the wells 6 and 6 ', and preferably has the same or larger diameter as the wells 6 and 6'.
As a material of the elastic sheet 11, a material having elasticity as appropriate, such as silicon rubber, fluororesin rubber, PDMS (polydimethylsiloxane: silicon resin), or the like can be selected. Moreover, it is necessary to select a material that does not hinder the reaction with respect to the reaction between the reaction reagent and the reaction reagent for the elastic sheet 11. When an appropriate material without reaction inhibition cannot be obtained, the necessary characteristics without reaction inhibition are ensured by coating the surface of the material as described above, surface treatment, surface modification, or the like.

  When the elastic sheet 11 is installed, it may be positioned and placed on the main body 2 or the lid 3 so that each hole 12 faces each well 6, 6 ′, or may be bonded. The elastic sheet 11 is sandwiched between the main body 2 and the lid 3, elastically deforms when a predetermined load is applied, and seals the wells 6 and 6 ′ in a liquid-tight manner. It is preferable that the flow path 9 communicating with 6 is elastic enough to seal the flow path 9 in a liquid-tight manner by deformation of the elastic sheet 11. The magnitude of the predetermined load applied to the elastic sheet 11 is a load that elastically deforms the elastic sheet 11 within a range that does not lose its elasticity, and the space such as the flow path 9 can be sealed in a liquid-tight manner. The load is such that the body 3 can withstand use.

The reaction vessel 1 according to the present embodiment has the above-described configuration, and the assembly method will be described with reference to FIG.
When assembling the reaction vessel 1, the elastic sheet 11 is placed on the inner surface of the main body 2 having the openings of the wells 6 and placed so that the holes 12 overlap the wells 6. At this time, the elastic sheet 11 is placed on the flow path 9 of the main body 2 in an overlapping manner. Then, the lid body 3 is folded back by the hinge portion 4 and overlapped on the elastic sheet 11. At this time, each well 6 ′ is closed so as to overlap with each hole 12 of the elastic sheet 11.
In integrating the reaction vessel 1, the main body 2, the elastic sheet 11, and the lid 3 may be bonded or welded to each other, or may be fixed with pins or the like. Alternatively, the main body 2 and the lid 3 may be bonded to each other with the elastic sheet 11 interposed therebetween.

The weight member 15 for fixing the reaction container 1 thus integrated with a load will be described with reference to FIGS. 4 and 5. FIG.
The weight member 15 includes a base 16 and a pressing member 17 that can be opened and closed by a hinge portion. The table 16 and the pressing member 17 are formed in a plate shape whose vertical and horizontal dimensions are slightly larger than those of the reaction vessel 1. The pressing member 17 shown in FIG. 4 is formed in, for example, a substantially rectangular flat plate, and a window portion 18 for accommodating the curved protruding portion of each well 6, 6 ′ protruding outward from the outer surface of the main body 2 or the lid 3 is formed on the inner surface thereof. Is formed.

This window part 18 has penetrated the outer surface on the opposite side to an inner surface as a through-hole, for example. On the inner surface of the presser member 17, presser blades 19 protrude from both sides of each window 18. The entire pressing member 17 may be formed of a transparent member. In that case, it is only necessary to form a recess for accommodating the well 6 or 6 ′ on the inner surface of the pressing member 17 instead of the window portion 18 formed of the through hole. Or you may form transparently only the part of the recessed part replaced with the window part 18 among the pressing members 17 toward the outer surface.
Further, the base 16 has substantially the same shape as the pressing member 17, and a through hole (or concave portion) is formed from the inner surface to the outer surface to accommodate the curved protrusions of the lid 3 or the wells 6 ′ and 6 of the main body 2. (Not shown).

Then, for example, the reaction vessel 1 is placed on the table 16 with the main body 2 facing upward, the pressing member 17 is closed, and the reaction vessel 1 is pressed between the table 16 by the pressing blade 19 with a predetermined load. Hold on. Next, the base 16 and the pressing member 17 are fixed with, for example, a plate-shaped fixing member 20 in a state in which the reaction vessel 1 is closed under a load. As a result, the reaction vessel 1 is held in a state where a predetermined weight is applied by the weight member 15.
In the weight member 15, the means for applying the load to the reaction vessel 1 can be appropriately selected and designed such as a means using a spring or fluid pressure, a means using a power source such as a motor, or a combination thereof. The weight may be applied from both sides of the reaction vessel 1 or from one side. The pressing member 17 may be plate-shaped or rod-shaped. The fixing member 20 may be engaged with and fixed to the opposite side surfaces of the weight member 15.
As shown in FIG. 3 (b), the reaction container 1 is elastically deformed between the main body 2 and the lid 3 in the reaction container 1 by applying a weight with the weight member 15, and the wells 6 and 6 ' The periphery 9 is liquid-tightly sealed and the flow path 9 communicating with the well 6 is also liquid-tightly sealed by the elastic sheet 11 elastically deformed by the pressing blade 19.

Next, an example of a reaction test method using the reaction container 1 according to the present embodiment will be described.
First, as shown in FIG. 2, with the reaction container 1 opened, for example, the reaction reagent is placed in each well 6 of the main body 2 (or the well 6 ′ of the lid 3).
As a reaction reagent, for example, an enzyme, a buffer solution, a fluorescent agent, a drug, an oligonucleotide, or the like can be used. In addition, in order to fix the reaction reagent in the well 6 (or well 6 ') on which the reaction reagent is placed, a substance such as wax or resin that changes its property in a liquid state in response to a temperature change can be added. Alternatively, when the reaction reagent is viscous, it can be fixedly held in the well 6 by using the viscosity of the reaction reagent itself instead of the substance whose properties change with temperature change. In addition, those whose properties change due to physical changes such as light and pressure, and those whose properties change chemically and biologically may be used.

Then, the elastic sheet 11 is installed on the main body 2 or the lid body 3, and the lid body 3 or the main body 2 is bent and closed by the hinge portion 4. In this state, as shown in FIG. 3 (a), since no load is applied to the reaction vessel 1, the lid 3 and the body 2 are in contact with the wells 6 and 6 'facing each other with the elastic sheet 11 interposed therebetween. Although it is in a state, the flow path 9 is not sealed.
Next, for example, a reaction reagent is introduced from the inlet 7 opened on the back surface of the main body 2 and supplied to each well 6 (or 6 ′) through the internal flow path 9. The reaction reagent may be supplied from the introduction port 7 before the main body 2 and the lid 3 are closed. As a reaction reagent, for example, a sample solution containing a human genome is used.
Then, as shown in FIG. 4, the reaction vessel 1 is placed on the base 16 of the load member 15 with the main body 2 facing upward, for example, and the main body 2 is covered with a pressing member 17 and pressed with a predetermined load. In this state, the free end portion or the free end portion of the base 16 and the pressing member 17 of the weight member 15 and the other end portion facing each other are fixed by the fixing member 20.

Then, as shown in FIG. 5, the reaction device 24 may be configured by installing heating / cooling means 22 for heating or cooling the reaction vessel around the weight member 15. By heating the reaction vessel 1 in a compressed state, the familiarity of the main body 2, the elastic sheet 11, and the lid body 3 can be improved and the adhesion can be improved. Therefore, mixing and mixing of the reaction liquid between the wells 6 during the reaction can be prevented with higher accuracy.
In addition, by heating the reaction vessel 1 with the heating / cooling means 22, for example, a substance such as wax or resin can be changed into a liquid state due to temperature change, and the reaction reagent and reaction reagent stored in the wells 6 and 6 ′. The main reaction can be promoted.
Further, a temperature measuring mechanism such as a thermocouple may be provided for measuring the temperature of the reaction vessel 1 and adjusting the temperature. In order to perform these temperature adjustments and temperature measurements, a computer equipped with a PLC (programmable logic controller), control software, or the like can be used.
Thus, each of the wells 6 and 6 ′ in the reaction container 1 is sealed in a liquid-tight manner by the elastic sheet 11 that is elastically deformed by receiving a predetermined load from the load member 15. Therefore, it is possible to prevent the occurrence of contamination by preventing the reaction reagent or the reaction reagent from diffusing into the adjacent wells 6 and 6 'during the main reaction.

As described above, according to the reaction container 1 according to the present embodiment, the elastic sheet 11 is sandwiched between the main body 2 and the lid 3 and is held in a pressurized state by the weight member 15, so , 6 'and the flow path 9 can be securely sealed, and the reaction vessel 1 can be prevented from being blocked. Therefore, during the main reaction, it is possible to prevent the reaction reagent and the reaction reagent from diffusing into the adjacent wells 6 and 6 ', thereby preventing the occurrence of contamination and measuring accurate reaction data.
In addition, when sealing the wells 6 and 6 'of the reaction vessel 1, the reaction vessel 1 is clamped by the weight member 15 as compared with the case in which the seal is permanently deformed and manually closed using a jig. Since 20 can hold | maintain in the state which predetermined load applied to the reaction container 1, reliable and stable obstruction | occlusion and sealing can be achieved.

The present invention is not limited to the reaction vessel 1 according to the above-described embodiment, and appropriate modifications can be made without departing from the spirit of the present invention. Next, other embodiments and modified examples of the present invention will be described, but the same or similar parts and members as those of the above-described embodiment are denoted by the same reference numerals, and the description thereof is omitted.
6 and 7 show a reaction vessel 26 according to the second embodiment of the present invention.
6A and 6B, a main body 27 and a lid 28 of the reaction vessel 26 are disclosed. 6A, for example, a plate-like main body 27 is formed with an elongated groove 30 having a substantially triangular cross section, for example, at the center, and the groove 30 is divided into a plurality of, for example, three wells 32 by a plate-like partition elastic material 31. The inlet 33 for introducing the reaction reagent and the air outlet 34 for venting air are disposed on both sides of the partition, and communicate with the outside.

The partition elastic material 31 is made of the same material as the elastic sheet 11 according to the first embodiment, for example. The partition elastic member 31 partitions each well 32 in a liquid-tight manner. The upper end of each partition elastic member 31 is formed at a height slightly lower than the opening of the concave groove 30, but it may be formed at the same height as the inner surface of the main body 27 or a height exceeding this.
6B is formed in a substantially plate shape, for example, but a recess may be formed in substantially the same manner as the recessed groove 30 of the main body 27. A rigid convex portion 36 made of, for example, the same material as that of the lid body 28 is formed to protrude from the lid body 28 at a position facing each partition elastic member 31 of the concave groove 30.
Then, as shown in FIG. 7, in the state where the main body 27 and the lid body 28 are aligned and closed, each well 32 of the concave groove 30 is elastically deformed by the partition elastic material 31 being pressed against the convex portion 36. It will be partitioned liquid-tight. As shown in FIGS. 6 and 7, the lid body 28 is formed with through holes 37 a and 37 b communicating with the introduction port 33 and the air port 34 in a state of being closed with the main body 27. Thus, the reaction reagent can be introduced from the through hole 37a with the main body 26 and the lid 28 of the reaction container 26 closed, and air can be discharged from the through hole 37b on the opposite side.

Therefore, in the reaction test method using the reaction container 26 according to the present embodiment, the reaction reagent is placed in each well 32 with the lid 28 of the reaction container 26 being opened from the main body 27, and the reaction reagent is further introduced from the inlet 33. As a sample solution containing the human genome. Then, with the main body 27 and lid 28 of the reaction container 26 closed, the reaction reagent is introduced into the introduction port 33 through the through hole 37a. When the introduction reagent 33 is filled with the reaction reagent, it passes through the partition elastic member 31 lower than the opening of the concave groove 30 and enters the well 32. Finally, the reaction reagent is supplied into the well 32 when it overflows into the air opening 34. Will be.
When all the wells 32 are filled with the reaction reagent, the main body 27 is pressurized and closed with the lid 28, and the wells 32 are partitioned liquid-tightly by the partition elastic material 31 and the projections 36.
Then, the reaction can be promoted by sandwiching the reaction vessel 26 with the weight member 15 to be in a pressurized state and heating or cooling with the heating / cooling means 22.
The reaction vessel 26 according to the present embodiment can also prevent contamination between the wells 32.

Next, the reaction container shown in FIG. 8 shows the reaction container according to the third embodiment, (a) is a plan view of the main body, and (b) is a central longitudinal sectional view of the reaction container.
The reaction vessel 38 according to this embodiment has almost the same configuration as the reaction vessel 26 according to the second embodiment, and only the differences will be described.
In FIG. 8, a packing-like elastic member 39 is attached to the entire circumference along a ridge line that partitions the opening of the concave groove 30 formed on the inner surface of the main body 27. Therefore, when the reaction reagent and the reaction reagent are put in each well 32 of the main body 27 and closed with the lid 28, the concave groove 30 is liquid-tightly sealed with the elastic member 39 and each well 32 is divided into the partition elastic material 31. Is partitioned liquid-tightly.

In the first embodiment described above, the wells 6 and 6 'are provided in both the main body 2 and the lid 3 of the reaction vessel 1, but as shown in the reaction vessels 26 and 38 of the second and third embodiments. For example, the well 6 (6 ′) may be provided only on one side, and the well may not be provided on the other side. In addition, although the recess-shaped wells 6, 6 'and 32 are formed as reaction chambers, it is only necessary that the reaction reagent can be held and reacted with the reaction reagent solution. There is no need to provide it, and it may be a flat surface without a recess as long as it can be fixed and held by wax or resin at the position where the reaction reagent is placed. Even in this case, the property of the wax or resin changes to a liquid state in response to the temperature, so that it can be reacted with the reaction sample solution.
In the first embodiment, the main body 2 is provided with the flow path 9 communicating with the well 6. However, the flow path 9 may be provided in the well 6 ′ of the lid 3 instead of the main body 2 or provided in both. Also good. Further, as shown in the reaction vessels 26 and 38 according to the second and third embodiments, the flow path 9 may not be provided at all.

Further, in the reaction vessels 1, 26, 38 shown in the first to third embodiments, the wells 6, 6 ', 32 are not limited to six or three, and one or a suitable plurality can be provided. Further, it is not necessary to provide an introduction port or an air port.
The elastic sheet 11, the partition elastic member 32, and the elastic member 39 constitute an elastic body. The elastic sheet 11 which is an elastic body is not necessarily limited to the configuration in which the hole portion 12 corresponding to the number of the wells 6 and 6 'is provided in the sheet-like member, and the shape of the elastic body is arbitrary. Second, as shown by the elastic member 39 and the partition elastic material 32 in the third embodiment, a linear elastic material may be formed in a lattice shape to constitute an elastic body. Further, the well 32 may not be liquid-tightly sealed around the entire periphery, and contamination may not occur by partitioning adjacent wells 32 in a liquid-tight manner as in the second embodiment.
FIG. 9 shows a modification of the reaction vessels 26 and 38 shown in the second and third embodiments. In the reaction vessels 26 and 38 shown in the second and third embodiments, the concave groove 30 provided in the main body 27 is provided with the introduction port 33 and the air port 34 on both sides of the plurality of wells 32,. As shown in FIG. 9, the number of wells may be increased by providing wells 32 and 32 instead of the introduction port 33 and the air port 34 on both sides of the groove 30. In this case, it is necessary to block the through holes 37a and 37b on both sides after the reaction reagent is supplied to the wells 32 from the through holes 37a.

Next, examples of the present invention will be described.
The examples are applied to the reaction vessel 1 according to the first embodiment of the present invention.
As each material of the reaction vessel 1, the main body 2 was made of aluminum, the lid 3 was made of transparent polypropylene, and the elastic sheet 11 was made of silicone rubber. The main body 2 and the lid 3 remove reaction inhibition, and a 70 μm-thick polypropylene sealant layer is provided and welded with a laser in order to bond the closed main body 2 and the lid 3.
In the main body 2 of the reaction vessel 1, a recess (dent) having a depth of 0.5 mm was formed as a well 6 to hold the reaction reagent. Examples of the reaction reagent include a buffer (buffer) used for PCR (polymerase chain reaction), a primer, an enzyme, an enzyme used for invader reaction (R), a buffer, a fluorescent label, and the like. AmpliWax (Applied Biosystems) having a melting point of about 65 ° C. was used as a substance to coat and fix the reaction reagent in the well 6. A sample solution containing a human genome was employed as the reaction reagent.

Then, a buffer (buffer solution) used for PCR (polymerase chain reaction) as a reaction reagent was dried and placed in the well 6 of the main body 2 in the reaction vessel 1 and covered and fixed with AmpliWax. The main body 2 and the lid 3 were closed with the elastic sheet 11 interposed therebetween, and a sample solution containing human genome was poured into each well 6 from the introduction port 7 through the flow path 9.
Then, the reaction vessel 1 was placed on the base 16 of the weight member 15 and sandwiched between the pressing members 17, and a load of 10 kg was applied from the vertical direction of the reaction vessel 1 and fixed with the fixing member 20. As a result, the elastic sheet 11 was elastically deformed, and each flow path 9 and each well 6, 6 'were sealed in a liquid-tight manner, and each well 6, 6' was independently closed. Heating / cooling means 22 is disposed outside the weight member 15 for heating.
Then, DNA was amplified under PCR conditions (that is, 95 ° C. for 1 minute 30 seconds, 60 ° C. for 30 seconds, 90 ° C. for 30 seconds, 60 ° C. and 90 ° C. for 30 times each). Thereafter, the temperature was kept at 60 ° C., an invader reaction was performed, and the fluorescence intensity could be measured correctly. Therefore, it was confirmed that no contamination occurred between the wells 6 and 6 in the reaction vessel 1.

The reaction container by 1st embodiment of this invention is shown, (a) is a top view, (b) is a side view. It is decomposition | disassembly explanatory drawing of the state which opened the reaction container shown in FIG. FIGS. 2A and 2B are partial cross-sectional views taken along line A-A in FIG. 1, in which FIG. 1A is a state in which the lid body and the main body are separated, and FIG. It is a perspective view of the state which mounted the reaction container on the stand in the state where the weight member was opened. It is a perspective view which shows the fixed state which clamped the reaction container with the weighting member and applied the load. It is a top view which shows the reaction container by 2nd embodiment of this invention, (a) is a main body, (b) is a cover body. FIG. 7 is a central longitudinal sectional view showing the reaction container in FIG. 6, where (a) shows a state in which the main body and the lid are separated, and (b) shows a state in which the main body and the lid are closed and pressurized. It is a figure which shows the reaction container by 3rd embodiment of this invention, (a) is a top view of a main body, (b) is the center longitudinal cross-sectional view of reaction container. It is a figure which shows the modification of the reaction container by 2nd, 3rd embodiment of this invention, (a) is sectional drawing which shows the isolation | separation state of a main body and a cover body, (b) is a longitudinal cross-sectional view of reaction container.

Explanation of symbols

1, 26, 38 Reaction vessel 2, 27 Main body 3, 28 Lid 6, 6 ′, 32 Well 7, 33 Inlet 9 Channel 11 Elastic sheet 12 Hole 15 Weight member 16 Base 17 Holding member 20 Fixing member 22 Heating -Cooling means 31 Partition elastic material 39 Elastic member

Claims (9)

  It has a main body and a lid that can be closed together, and has a reaction chamber in which a reaction reagent is placed in at least one of the main body and the lid, and an elastic body is interposed between the main body and the lid. A reaction vessel characterized by that.   The reaction container according to claim 1, wherein the elastic body is disposed so as to surround the reaction chamber.   The reaction container according to claim 1, wherein the elastic body is hydrophobic.   The reaction container according to claim 1, wherein a reaction reagent flow path is connected to the reaction chamber.   The reaction container according to any one of claims 1 to 4, wherein at least one of the main body and the lid is provided with a reaction reagent introduction port and / or an air port for venting air.   A reaction apparatus comprising heating / cooling means for heating or cooling the reaction vessel according to any one of claims 1 to 5.   A reaction chamber is provided in at least one of the main body and the lid, and the reaction chamber is liquid-tight by closing the main body and the lid with an elastic body and elastically deforming the elastic body. A method for closing a reaction vessel, wherein the method is sealed.   The flow path communicating with the reaction chamber is formed in at least one of the main body and the lid, and the flow path is liquid-tightly sealed by elastically deforming the elastic body. Method of closing the reaction vessel.   The method for closing a reaction vessel according to claim 7 or 8, wherein the closed reaction vessel is heated or cooled.

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