JPH047759Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a culture device. More specifically, the invention particularly relates to a culture device for anaerobic or aerobic culture of microorganisms or cells.

[Prior Art] There are two types of microorganism or cell culture: aerobic culture performed in an open system, and culture performed in a closed system as a hermetically sealed or anaerobic culture.

Conventionally, culturing in a sealed system involves covering the main body of a shear dish or multi-well plate with a plurality of culture depressions with a lid.
Adhesive tape is pasted on the outer periphery of the sides to seal the gap, or the main body is placed in another sealable container to make it airtight.

However, when using adhesive tape such as the former, if the culture medium stored in the recess is liquid,
If the main body is not held horizontally, the culture medium will spill out, making it difficult to apply adhesive tape to the outer periphery of the sides and requiring skill. Furthermore, when storing the product in another container as in the latter case, the work is not easy.

On the other hand, especially when using a multi-well plate as the main body, an adhesive sheet is directly adhered onto the culture recesses or culture holes called wells to seal the top of each well.

However, in this case, since the adhesive sheet is located above the well and the adhesive on the sheet faces the culture medium, there is a risk that the culture will be affected by the adhesive. In addition, if the top surface of the well wall is wet or the top surface is narrow, it is difficult to completely seal the well, and since culture is performed under high humidity conditions,
Part of the adhesive sheet may peel off during culturing.

Furthermore, there is no known container that can be easily used for both aerobic culture and anaerobic culture.

[Problems to be Solved by the Invention] The present invention was devised in view of the above-mentioned circumstances, and its main purpose is to provide a culture device that eliminates the above-mentioned drawbacks and inconveniences. More specifically, it not only has a simple structure and can be sealed extremely easily and reliably, but also
It is an object of the present invention to provide a culture device that can be used for aerobic culture very easily.

[Means for Solving the Problems] The inventor of the present invention has repeatedly conducted various studies regarding the above-mentioned objectives, and as a result, has come up with the present invention.

That is, the present invention has a main body having at least one recess for culturing microorganisms or cells, and a lid covering the main body, and a groove is provided on the outer side surface of the main body or the inner side surface of the main body, An elastic ring is removably fitted into the groove, and through the elastic ring,
The main body and the lid are configured to airtightly engage with each other to enable anaerobic culture in a sealed system, and when the elastic ring is removed and the lid is fitted onto the main body, the lid can be opened. This culture device is characterized by being capable of culturing in a system.

Hereinafter, the present invention will be explained in detail according to the embodiments shown in FIGS. 1 to 3.

The culture device of the present invention has a main body 1. Main body 1
It may be in the shape of a shear plate having a recess for culturing, or it may be in the shape of a multi-hole plate having a large number of recesses 2 for culturing, ie, wells, as shown in the figure. The structure, shape, material, etc. thereof can be varied.

The culture device of the present invention has a lid body 4 that covers the main body 1.
has. There are no particular restrictions on the shape, structure, material, etc. of the lid 4, and its dimensions may be such that the lid 4 can be airtightly engaged with the main body 1 via an elastic ring, which will be described later.

On the outer side surface of the main body 1 or the inner side side of the lid,
An elastic ring 3 is provided.

Although the elastic ring 3 can be provided in various arrangements, it is most convenient to provide the elastic ring 3 so as to extend at least once around the outer side surface 15 of the main body as shown in the drawing.

Alternatively, an elastic ring may be provided on the inner surface of the side portion of the lid body 4.

With the elastic ring arranged in this way, when the lid 4 is fitted to the main body 1 as shown in FIG. A sealing system is formed between the lid body 4 and the lid body 4.

In this case, the material of the elastic ring 3 is as follows:
Natural or synthetic rubber, silicone rubber, etc. can be used, and among these, silicone rubber is most suitable.

On the other hand, in order to provide the elastic ring on the side outer surface 15 of the main body 1, in the present invention, instead of gluing the two together, a groove into which the elastic ring 3 is fitted is formed so as to go around at least once around the side outer surface 15 of the main body 1. At least one groove 151 is provided, and an elastic ring 3 is inserted in this groove 151.
It is fitted in a removable manner. By doing so, the elastic ring does not shift from the side outer surface 15 of the main body 1 during use;
There is an advantage that the elastic ring can be removed and used as an open aerobic culture device. Alternatively, a groove may be provided on the inner surface of the side portion of the lid body 4, and an elastic ring may be removably inserted and fixed into this groove.

In addition, the culture device of the present invention simply uses the elastic ring 3.
It can also be used as an aerobic culture device in an open system by simply removing it. At this time, as shown in FIG. 3, for example, by providing ribs, grooves, notches, etc. on the inner surface of the lid 4 and the four corners of the upper end surface of the main body 1, a communication path between the outside and the inside is formed. It is possible to ensure communication with the outside world.

[Specific functions and effects of the invention] The culture device of the invention is used by storing a culture medium in the recess 2, placing microorganisms or cells therein, and then fitting the lid 4 onto the main body 1.

At this time, the inner surface of the lid body 4 is airtightly engaged with the side outer surface 15 of the main body 1 via the elastic ring 3.
The sealing performance is reliable and good, and outside air does not flow into the interior.

In this case, the seal will not be broken during culture.

In addition, since the lid 4 can be sealed simply by placing it over the main body 1, the degree of sealing is not only high, but also the sealing work is easy.

Furthermore, the structure itself is simple, and since the main body 1 and the lid 4 are connected via the elastic ring 3, there is no need for a means for fixing the lid 4 and the main body 1. The structure is also simplified.

Furthermore, there is no adverse effect on culture unlike when sealing is performed using adhesive tape.

In addition, since the elastic ring is fitted into the groove formed on the side outer surface 15 of the main body 1, the groove will not fall off even after multiple uses. It is also possible to use

These effects can be achieved in the same way whether the elastic ring is provided on the inner surface of the side of the lid or on the outer surface of the main body.

[Brief explanation of the drawing]

Figures 1 to 3 are diagrams for explaining an embodiment of the present invention, of which Figure 1 is a perspective view of the main body constituting the culture device, Figure 2 is a sectional view of the main body, and Figure 3 is a cross-sectional view of the main body. The figure is a sectional view of a culture device constructed by combining a main body and a lid. Explanation of symbols, 1...Body, 2...Recess, 3...
Elastic ring, 4...lid body, 15...main body side outer surface, 151...groove.

Claims (1)

[Scope of utility model registration request] It has a main body having at least one recess for culturing microorganisms or cells, and a lid covering the main body,
A groove is provided on the outer side surface of the main body or the inner side surface of the lid, and an elastic ring is removably fitted into the groove, and the main body and the lid are airtightly engaged through the elastic ring. When the elastic ring is removed and the lid is fitted onto the main body, culture can be performed in an open system. culture equipment.