JPH0621360Y2 - Tissue culture vessel - Google Patents

Description

[Detailed description of the device]

[0001]

This invention relates to a tissue culture container for culturing plant tissue.

[0002]

2. Description of the Related Art Conventional tissue culture vessels 100a, 100
As shown in FIGS. 11 to 13, b and 100c were known to be flask-shaped, beaker-shaped, and petri dish-shaped. The flask-shaped tissue culture container 100a has an upper opening 1a.
The culture medium 2a was put in, the tissue to be cultured 3a was placed thereon, and the degreased surface and the sponge stopper 50a were used.

A beaker-shaped tissue culture container 100b is provided with a culture medium 2b through an upper opening 1b, and a culture medium 3b is placed thereon.
Was placed and the rubber plug 50b was used. The petri dish-shaped tissue culture container 100c was used by placing the medium 2c through the upper opening 1c, placing the cultured tissue 3c thereon, and using the upper pig 50c.

[0004]

The flask-shaped tissue culture vessel 100a has been most frequently used in the past, but it is difficult to put the medium 2a and the tissue 3a to be cultured in and out due to the small area of the opening 1a. There was a problem. or,
There is a problem that the usable area is smaller than the area of the medium 2a in the cultured tissue 3a, which has a shape that is narrowed upward and grows strongly vertically upward. Also, this container 100a
Can be placed horizontally, but they cannot be placed vertically one on top of the other, and a shelf is required to arrange them vertically, so the culture room, which is a space narrower than the shelf, cannot be fully used. was there.

The beaker-shaped tissue culture container 100b has a large opening 1b and is easy to use. However, when the opening 1b is closed with the rubber stopper 50b, the opening 1b and the rubber stopper 50b are closed.
There was a problem that various bacteria easily propagate near the engaging part with. Also, in order to close the opening 1b with the rubber stopper 50b,
There is a problem that ventilation is not performed between the inside and the outside of the container. Further, there is a problem that a shelf is required to arrange the shelves in the vertical direction.

Since the height of the dish-shaped tissue culture vessel 100c is limited, the amount of the medium 2c contained in the vessel is extremely small, which results in a problem that the culture capacity is small and that the culture to be grown grows vertically upward. There is a problem that it cannot be used for the tissue 3c. In addition, there is a problem that ventilation is not performed between the opening 1c and the upper lid 50c, or the ventilation amount cannot be adjusted even if ventilation is performed.

[0007]

[Constitution of device] The structure of this device is composed of a substantially cubic shape,
The bottom wall and the upper wall are substantially horizontal, and the front wall intersects the bottom wall at a predetermined angle and intersects with each other.
The main body has a 100 mm opening, a short cylindrical neck is erected from the periphery of the opening, and a male thread is formed on the outer wall of the body of the neck. An upright edge portion, which is a tubular member penetrating the disk portion, one end of which is projected from the surface of the disk portion, and the other end of which is provided with a ventilation portion communicating with the back surface side of the disk portion, Has a female screw portion that can be screwed with the male screw portion on the inner wall of the body portion, and a lid body that is detachably screwed to the neck portion by this, and is filled in the cylindrical ventilation portion to adjust the ventilation amount. A tissue culture vessel comprising a breathable filler.

More specifically, since the area of the opening 1 has a size of about 20 to 80 cm ₂ , it is extremely easy to insert and remove the medium 2 and the tissue to be cultured 3, and the opening 1 Since the surface has an angle of 60 ° to 90 ° with respect to the vertical, it is easy to put the medium 2 and the tissue to be cultured 3 in and out, and prevent bacteria and foreign substances from dropping into the container from above, and Breeding does not occur, aeration is performed between the inside and the outside of the container, and the amount of ventilation can be adjusted, the containers can be stacked, and a shelf is not required when arranging them in the vertical direction. The tissue culture container has a greater culturing capacity than that of 10 to 30 mm and can be used even when the tissue 3 to be cultured grows vertically upwardly to a maximum height of 110 mm. .

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The invention will be described with reference to the embodiments shown in FIGS. 1 to 10, but the invention is not limited thereto. As shown in FIG. 1, the tissue culture container 100 of the present invention comprises a bottom wall 4, an upper wall 5, two side walls 6, 6 ′, a rear wall 7, a front wall 8,
It comprises a neck 9 protruding from the front wall and having an opening 1 and a lid 50 closing the opening 1.

The bottom wall 4 and the top wall 5 and the two side walls 6 and 6'are parallel to each other, and the two side walls 6 and 6'and the above-mentioned bottom wall 4 and top wall 5 intersect vertically. The rear wall 7 is perpendicular to the bottom wall 4, the top wall 5 and the two side walls 6, 6 ', and the front wall 8
Intersects with the bottom wall 4 at an angle of 75 ° and inclines upward. Also, the joint edge of each wall is curved,
Here, the container body 30 is formed.

The height H10 of the side walls 6 and 6'is 103 mm, and the width W11 and depth L12 of the bottom wall 4 are 103 mm and 115 mm, respectively. The front wall 8 has a neck portion 9 formed at a height of 25 mm from the bottom wall 4 and protruding vertically from the front wall 8 to form a male thread portion 13 for screwing. The end portion of the neck portion 9 has a radius of 28 mm, that is, the opening 1 having an area of about 2500 mm ² . A lid 50 is screwed onto and removed from the opening 1 via a packing 14, and the lid 50 has an inner diameter of 3 mm and a length of 29 mm perpendicular to the surface of the lid at the center.
Has a pipe-shaped vent hole 15. Reference numeral 16 denotes a female screw portion of the lid body 50 for receiving the male screw portion 13, and a thin anti-slip line is attached to the entire circumference of the lid body.

The container body 30 has a thickness of about 1 mm and is made of transparent polycarbonate resin (blow molding). The packing 14 has an outer diameter of 69 mm, an inner diameter of 50 mm and a thickness of 1 mm, and is made of silicone rubber. The lid 50 has an outer diameter of 76 mm and has a thickness of approximately 2 except for the height of the thread of the female thread 16 and a thin line.
Made of polyethylene resin in mm.

The tissue culture container 100 of the present invention is formed as described above. Therefore, its usage is as follows, and an example of its emergence is shown using asparagus as the cultured tissue 3. First, set the container body 30, the lid 50 and the packing to 120 ° C.
Leave for 60 minutes under steam to completely sterilize. Next, culture medium 2 in which agar is impregnated with nutrients and plant hormones that promote germination
Through the opening 1 to the inside of the main body 30 with a thickness of about 20 mm to fill.

After the medium 2 has cooled to a predetermined temperature (for example, 20 ° C.), the dew on the inner wall of the main body 30 is wiped off with sterilized and dry tissue paper. A clean bench for transplanting the tip of the stem of the male plant of asparagus placed in the tissue culture container 100 and the dish prepared in this way (with an operation box having a glass door that opens and closes at the front, It can be opened halfway and the transplanter can perform the transplantation manual work in the box through the lower opening.) 20, and the glass door 21 of the clean bench 20 is lowered so that the worker can perform the manual work. The operator uses a gas burner (not shown) in the clean bench to sterilize a main part of the tissue culture container 100, for example, the opening 1 by stacking.

Then, the tip of the stem of the male strain of asparagus, which is the tissue to be cultured 3, is placed at a predetermined position of the medium 2 inside the main body 30 through the knob opening 1 with the pincette. The lid 50 having the packing 14 mounted thereon is screwed onto the neck portion 9 of the main body 30 to complete the transplanting operation. At the time of this transplantation, it is desirable that the inside of the clean bench is shielded from the outside as much as possible in order to protect the aseptic condition.
Should be as low as possible. In this tissue culture container 100, the opening 1 is opened in the lateral direction, and the movement of the worker's hand is substantially horizontal in the transplanting work. Therefore, the glass door 21 of the clean bench 20 is opened by the amount of the worker's arm. The transplant work is possible only by itself. Therefore, the work is simple and the sterile condition is well protected.

After that, the tissue culture container 100 containing the tissue to be cultured 3 is taken out from the clean bench, and the temperature, humidity,
The asparagus is cultivated by moving to a sterile culture room where lighting and other conditions are set to the prescribed environmental conditions. At this time, the tissue culture container 1
As described above, 00 can be stacked and arranged because the bottom wall 4 and the upper wall 5 are parallel to each other.

When culturing is continued for about 1 month, asparagus grows like hair buds. Here, the buds are transferred to another tissue culture vessel containing a growth medium containing a hormone of special composition for growing. At this time, the sprouted asparagus is taken out from the opening 1 using tweezers, and then transferred into another tissue culture container containing a growth medium through the opening. After this, asparagus sprouts grow 5 to 10 times in culture for about one month.

The tissue culture container 100 is used as described above. However, since the two side walls 6, 6'and the rear wall 7 are perpendicular to the bottom wall 7, the medium area can be widely used.
Further, since the container 30 has a sufficient height H10, it can also be used for the tissue to be cultured 3 that grows strongly vertically upward.
Further, since the area of the opening 1 is sufficiently large, the medium 2 and the tissue to be cultured 3 can be easily put in and taken out, and the fact that the surface of the opening 1 is slightly upward means that the container 100 is placed on the table of the clean bench 20. Place the cultured tissue 3 on the container 10
It is very suitable for wrist movement when putting in and out of 0. In addition, the amount of the medium 2 is sufficient and the culturing ability is extremely large.

A vent hole 15 is provided in the lid 50 to allow ventilation inside and outside the container 100.
The air flow rate can be adjusted by packing a breathable filler, for example, glass fiber, a foamed resin composed of open cells, specifically, a plug having a specific structure made of silicon foam, ceramics, or the like.

Further, the lid 50 and the packing 14 are easy to sterilize, and no germs propagate in the vicinity of the screwed portion with the main body 30 and airtightness can be maintained. The tissue culture container can be used not only when the tissue to be cultured is a plant but also when it is an animal. Also, the opening can be used as the upper surface.

[0021]

[Effects of the Invention] This device has a large utilization area relative to the culture medium area, has sufficient culturing capacity, can be used regardless of the type of tissue to be cultured, can easily put in and out the medium and the tissue to be cultured, and can propagate various bacteria. It is possible to provide a tissue culture container that can be stacked and can be ventilated both inside and outside.

[Brief description of drawings]

FIG. 1 is a perspective view of a tissue culture container according to the present invention.

FIG. 2 is a side view having a partial cutout showing the stacking use state of FIG. 1;

FIG. 3 is a perspective view showing a usage state of FIG.

FIG. 4 is a front view of FIG.

5 is a right side view of FIG.

FIG. 6 is a plan view of FIG.

FIG. 7 is a rear view of FIG.

FIG. 8 is a bottom view of FIG.

9 is a side vertical cross-sectional view illustrating the structure of FIG.

FIG. 10 is an explanatory view showing a transplanting work according to FIG.

FIG. 11 is a perspective view of a conventional flask-shaped tissue culture container.

FIG. 12 is a perspective view of a conventional beaker-shaped tissue culture container.

FIG. 13 is a perspective view of a conventional petri dish tissue culture container.

[Explanation of symbols]

100 tissue culture container, 30 container body, 50 lid,
1 opening, 2 medium, 3 non-cultured tissue, 4 bottom wall, 5
Top wall, 6 side walls, 7 rear wall, 8 front wall, 9 neck, 10
Height H, 11 Width W, 12 Depth L, 13 Male part, 14 Packing, 15 Vent hole, 16 Female part,
20 clean bench, 21 glass door

Claims (1)

[Scope of utility model registration request] 1. A substantially cubic body in which a bottom wall and a top wall are substantially horizontal, the front wall intersects the bottom wall at a predetermined angle, and the front wall has a diameter of 50 to 100 mm. It has an opening and a short cylindrical neck is erected from the periphery of the opening.
A main body in which a male thread portion is formed on the outer wall of the body portion of the neck portion, a disc portion, and an edge portion which is erected from the outer peripheral edge in a belt-like shape,
A cylindrical member penetrating the disk portion, one end of which is projected from the surface of the disk portion, and the other end of which is provided with a ventilation portion that communicates with the back surface of the disk portion. It has a female screw part that can be screwed with the male screw part, and by this, a lid body that is detachably screwed with the neck part, and a breathable filling material that is filled in the tubular ventilation portion and adjusts the ventilation amount. Tissue culture vessel.