JPH0548113B2 - - Google Patents
Info
- Publication number
- JPH0548113B2 JPH0548113B2 JP23775186A JP23775186A JPH0548113B2 JP H0548113 B2 JPH0548113 B2 JP H0548113B2 JP 23775186 A JP23775186 A JP 23775186A JP 23775186 A JP23775186 A JP 23775186A JP H0548113 B2 JPH0548113 B2 JP H0548113B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- temperature
- filamentous fungi
- culture tank
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003507 refrigerant Substances 0.000 claims abstract description 27
- 238000001816 cooling Methods 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 241000233866 Fungi Species 0.000 claims description 48
- 238000012258 culturing Methods 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 13
- 241000228347 Monascus <ascomycete fungus> Species 0.000 abstract description 2
- 241000228212 Aspergillus Species 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 14
- 239000002609 medium Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 7
- 239000002826 coolant Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000002518 antifoaming agent Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明は培養槽内で糸状菌を培養する方法、
特に液体培地を用いて糸状菌を液体培養する方法
に関するものである。[Detailed Description of the Invention] [Industrial Application Field] This invention provides a method for culturing filamentous fungi in a culture tank;
In particular, it relates to a method for liquid culturing filamentous fungi using a liquid medium.
第４図は例えば産業図書(株)発行「バイオリアク
ター」に記載された従来の培養装置を示す断面図
である。図において、１は培養槽で、堅形円筒状
に形成され、内部に培養液２を入れて培養を行う
ようになつている。培養槽１の培養液２の液面３
より下部の外周には温度調節用ジヤケツト４が設
けられ、冷熱媒出入口５，６が上下に連絡してい
る。また培養槽１内の液面下にはコイル７が設け
られ、上下に冷熱媒出入口８，９に連絡してい
る。培養槽１には培養液中で回転する攪拌機１０
が設けられ、その下側に通気管１１が開口してい
る。培養槽１の上部には仕込口１２、のぞき窓１
３、接種口１４、排気口１５および光取入口１６
が設けられており、下部には収穫口１７が設けら
れるとともに、液輸送管１８が開口している。１
９は培養槽１の内壁に等間隔で放射状に設けられ
た邪魔板、２０は試料採取管である。
FIG. 4 is a cross-sectional view showing a conventional culture device described in "Bioreactor" published by Sangyo Tosho Co., Ltd., for example. In the figure, reference numeral 1 denotes a culture tank, which is formed into a rigid cylindrical shape, into which a culture solution 2 is placed for culturing. Liquid level 3 of culture solution 2 in culture tank 1
A temperature regulating jacket 4 is provided on the outer periphery of the lower part, and cooling/heating medium inlets and outlets 5 and 6 communicate with each other vertically. Further, a coil 7 is provided below the liquid level in the culture tank 1, and communicates with cooling medium inlets and outlets 8 and 9 above and below. The culture tank 1 includes a stirrer 10 that rotates in the culture solution.
is provided, and a ventilation pipe 11 is opened on the lower side thereof. At the top of the culture tank 1 there is a feeding port 12 and a peephole 1.
3. Inoculation port 14, exhaust port 15 and light intake port 16
A harvesting port 17 is provided at the bottom, and a liquid transport pipe 18 is opened. 1
9 is a baffle plate provided radially on the inner wall of the culture tank 1 at equal intervals, and 20 is a sample collection tube.
上記の培養装置において糸状菌の培養を行う方
法は、仕込口１２から培養槽１に培養液２を仕込
み、接種口１４から糸状菌を接種し、攪拌機１０
を回転させて培養液２を攪拌し、ジヤケツト４お
よび（または）コイル７に冷媒または熱媒を導入
して培養液２を冷却または加温して培養適温に維
持し、糸状菌の培養を行う。培養適温に維持する
ために冷却を要する場合には、冷熱媒出入口６お
よび（または）９から冷却水等の冷媒を導入し、
冷熱媒出入口５および（または）８から排出す
る。加温する場合は逆方法に温水、蒸気等の熱媒
を導入、排出する。培養を行つた培養液は連続
的、間欠的またはバツチ式に収穫口１７から取出
す。 The method for culturing filamentous fungi in the above-mentioned culture device is to charge the culture solution 2 into the culture tank 1 from the charging port 12, inoculate the filamentous fungi from the inoculation port 14, and use the stirrer 10.
The culture solution 2 is stirred by rotating, and a refrigerant or heat medium is introduced into the jacket 4 and/or the coil 7 to cool or warm the culture solution 2 and maintained at an appropriate culture temperature, thereby culturing the filamentous fungi. . When cooling is required to maintain the culture at an appropriate temperature, a coolant such as cooling water is introduced from the coolant inlet/outlet 6 and/or 9,
It is discharged from the cooling/heating medium inlet/outlet 5 and/or 8. When heating, a heat medium such as hot water or steam is introduced and discharged using the reverse method. The culture solution used for culturing is taken out from the harvest port 17 continuously, intermittently, or in batches.
上記のような従来の培養装置においては、発泡
に対する対策は機械消泡材、消泡剤の自動添加等
により可能であるが、培養微生物の壁面付着に関
しては防止策がとられていないため、糸状菌の培
養の場合には、培養槽１の培養液２の液面３より
上の上部壁面２１に菌塊２２が付着して生長す
る。
In the conventional culture equipment described above, countermeasures against foaming can be taken by using mechanical antifoaming agents and automatic addition of antifoaming agents, but since no measures have been taken to prevent cultured microorganisms from adhering to walls, filamentous In the case of culturing bacteria, a bacterial mass 22 adheres to the upper wall surface 21 above the liquid level 3 of the culture solution 2 in the culture tank 1 and grows.
微生物の壁面付着は糸状菌に特に発生しやすい
問題で、糸状菌の液体培養を困難にしている大き
な要因である。このような糸状菌の壁面付着は培
養液２の液面３付近に起こりやすく、液面３の1/
２以上を覆い、かつ壁面２１の全面に付着するこ
ともある。したがつて培養槽１の上部より添加さ
れるPH調整剤や栄養源が培養液２にまで到達しな
いこともある。 Adhesion of microorganisms to walls is a problem that is particularly likely to occur with filamentous fungi, and is a major factor that makes liquid culture of filamentous fungi difficult. Such adhesion of filamentous bacteria to the wall surface tends to occur near the liquid level 3 of the culture solution 2, and
It may cover two or more areas and adhere to the entire surface of the wall surface 21. Therefore, the PH adjuster and nutrient source added from the top of the culture tank 1 may not reach the culture solution 2.
また菌塊２２が壁面２１に付着すると、培養液
中の菌体濃度が減少し、極端な場合には大部分の
菌体が壁面２１に付着し、培養液２中には存在し
なくなることもある。このため、培養時間が長
くなる、生産物の生成速度が低下する、培養
の再現性が悪くなる、付着菌体が死滅腐敗し、
培養条件を狂わせるなどの問題点があつた。 Furthermore, when the bacterial mass 22 adheres to the wall surface 21, the concentration of bacterial cells in the culture medium decreases, and in extreme cases, most of the bacterial cells may adhere to the wall surface 21 and no longer exist in the culture medium 2. be. As a result, the culture time becomes longer, the production rate of the product decreases, the reproducibility of the culture deteriorates, and the attached bacterial cells die and rot.
There were problems such as disrupting the culture conditions.
この発明は上記問題点を解決するためのもの
で、培養槽壁面への菌塊の付着がなく、効率よく
培養を行うことが可能な糸状菌の培養方法および
装置を提供することを目的としている。 This invention is intended to solve the above-mentioned problems, and aims to provide a method and apparatus for culturing filamentous fungi that can be efficiently cultured without the adhesion of bacterial masses to the wall surface of the culture tank. .
この発明は次の糸状菌の培養方法およびその装
置である。
The present invention provides the following method and apparatus for culturing filamentous fungi.
(1) 培養槽内で糸状菌を培養する方法において、
培養槽内の培養液の液面より上部の壁面を前記
糸状菌の培養適温より低い温度に維持して培養
するとを特徴とする糸状菌の培養方法。(1) In a method of culturing filamentous fungi in a culture tank,
1. A method for culturing filamentous fungi, which comprises culturing while maintaining a wall surface above the liquid level of a culture solution in a culture tank at a temperature lower than the optimum culture temperature for the filamentous fungi.
(2) 糸状菌を培養する培養槽と、この培養槽内の
培養液を前記糸状菌の培養適温に維持する装置
と、前記培養槽の培養液の液面より上部の壁面
を前記糸状菌の培養適温より低い温度に維持す
る装置とを有することを特徴とする糸状菌の培
養装置。(2) A culture tank for culturing filamentous fungi, a device for maintaining the culture solution in this culture tank at an appropriate temperature for culturing the filamentous fungi, and a wall surface above the liquid level of the culture solution in the culture tank for cultivating the filamentous fungi. 1. A device for cultivating filamentous fungi, comprising a device for maintaining the temperature at a temperature lower than the optimum culture temperature.
本発明において培養の対象となる糸状菌として
は特に制限はなく、アルペルギルス属、ペニシリ
ウム属、ムコール属、リゾープス属、モナスカス
属等に属する糸状菌のすべてが培養の対象とな
る。培養培地としては通常の糸状菌培養に用いら
れる液体培地が使用でき、粘度が高いものでもよ
い。 The filamentous fungi to be cultured in the present invention are not particularly limited, and all filamentous fungi belonging to the genus Alpergillus, Penicillium, Mucor, Rhizopus, Monascus, etc. can be cultured. As the culture medium, a liquid medium commonly used for culturing filamentous fungi can be used, and one with high viscosity may be used.
培養条件は通常の糸状菌培養に採用されている
条件がそのまま採用でき、糸状菌の種類（属、
種、株等）によつて異なるが、一般的には培地PH
３〜８、培養温度20〜40℃、培養時間15時間程度
以上である。 The culture conditions can be the same as those used for normal filamentous fungus cultivation, and the types of filamentous fungi (genus,
Although it varies depending on the species, strain, etc.), in general, the culture medium PH
3 to 8, the culture temperature is 20 to 40°C, and the culture time is about 15 hours or more.
本発明ではこのような糸状菌の培養を培養槽で
行う場合、培養槽内の培養液を糸状菌の培養適温
に維持するとともに、培養液の液面より上部の壁
面を上記培養適温より低い温度に維持して培養を
行う。培養適温は糸状菌の種類によつて異なる
が、一般的には上記培養温度である20〜40℃の範
囲に入る。培養適温より低い温度はこの温度より
低い温度であるが、一般的には15℃以下が好まし
く、10℃以下が特に好ましい。 In the present invention, when culturing such filamentous fungi in a culture tank, the culture solution in the culture tank is maintained at an appropriate temperature for culturing the filamentous fungi, and the wall surface above the surface of the culture solution is kept at a temperature lower than the above-mentioned optimum culture temperature. The cells are maintained and cultured. The suitable culture temperature varies depending on the type of filamentous fungus, but generally falls within the above culture temperature range of 20 to 40°C. The temperature lower than the optimum culture temperature is a temperature lower than this temperature, but generally 15°C or lower is preferable, and 10°C or lower is particularly preferable.
培養槽内の培養液を糸状菌の培養適温に維持す
る装置としては通常採用されている冷却または加
温用のジヤケツト、コイル等があげられる。培養
液の液面より上部の壁面を糸状菌の培養適温より
低い温度に維持する装置としては、冷却用ジヤケ
ツト、冷却用コイル、冷媒噴出ヘツダ等があげら
れる。 Devices for maintaining the culture solution in the culture tank at an appropriate temperature for culturing filamentous fungi include commonly used cooling or heating jackets, coils, and the like. Devices for maintaining the wall surface above the liquid level of the culture liquid at a temperature lower than the optimum temperature for culturing filamentous fungi include cooling jackets, cooling coils, refrigerant jetting headers, and the like.
培養液を糸状菌の培養適温に維持し、培養液の
液面より上部の壁面を培養適温より低い温度に維
持して糸状菌の培養を行うと、攪拌等により上部
壁面に菌体が付着しても、壁面が培養適温より低
い温度に維持されているので、上部壁面における
糸状菌の増殖はなく、菌塊は付着しない。一方、
培養液は培養適温に維持されているため、培養液
中で糸状菌が増殖し、効率のよい培養が行われ
る。
When culturing filamentous fungi by maintaining the culture solution at an appropriate temperature for culturing filamentous fungi and maintaining the wall surface above the liquid level of the culture solution at a temperature lower than the optimum culture temperature, bacterial cells will adhere to the upper wall surface due to stirring, etc. However, since the wall surface is maintained at a temperature lower than the optimum culture temperature, there is no growth of filamentous fungi on the upper wall surface and no bacterial mass adheres. on the other hand,
Since the culture solution is maintained at an appropriate temperature for culture, filamentous fungi grow in the culture solution, resulting in efficient culture.
以下、この発明を図面の実施例によつて説明す
る。第１図ないし第３図は別の実施例による培養
装置を示す断面図であり、第４図とほぼ同様の構
造となつているが、細部を省略して概略的に図示
されており、第４図と同一符号は同一または相当
部分を示している。
Hereinafter, the present invention will be explained with reference to embodiments shown in the drawings. FIGS. 1 to 3 are cross-sectional views showing a culture device according to another embodiment, which has almost the same structure as FIG. 4, but is schematically illustrated with details omitted. The same reference numerals as in FIG. 4 indicate the same or corresponding parts.
培養槽１は培養液２を糸状菌の培養適温に維持
する装置として、培養液２の液面３より下部の外
周に温度調節用ジヤケツト４が設けられて、冷熱
媒出入口５，６が上下に連絡しているのは従来の
ものと同様であるが、培養液２の液面３より上部
の上部壁面２１を糸状菌の培養適温より低い温度
に維持する装置として、第１図では上部壁面２１
の外周に冷却用ジヤケツト２３が設けられ、その
上部に冷媒出口２４、下部に冷媒入口２５が連絡
している。第２図では上部壁面２１の外周に冷却
用コイル２６が巻かれて、冷媒出口２４および冷
媒入口２５に連絡しており、冷却用コイル２６は
カバー２７内に充填されたガラスウール等の保冷
材２８により覆われている。第３図では培養槽１
内の上部に冷媒噴出ヘツダ２９が設けられ、冷媒
管３０の冷媒を上部壁面２１に噴射するようにな
つている。冷媒管３０の冷媒としては専用の冷媒
のほかに、２点鎖線で示すように培養液または上
部のガスを循環系３１から冷却装置３２で冷却し
て循環するようにしてもよい。第１図ないし第３
図では培養液２中のコイル７は図示されていない
が、これはあつてもなくてもよい。Ｍは攪拌機１
０を回転させるモーターを示す。他の構成は第４
図と同様である。 The culture tank 1 is a device for maintaining the culture solution 2 at an appropriate temperature for culturing filamentous fungi, and is equipped with a temperature control jacket 4 on the outer periphery below the liquid level 3 of the culture solution 2, with cooling medium inlets and outlets 5 and 6 arranged vertically. The communication is the same as that of the conventional one, but in FIG.
A cooling jacket 23 is provided on the outer periphery of the cooling jacket 23, with a refrigerant outlet 24 communicating with the upper part and a refrigerant inlet 25 communicating with the lower part. In FIG. 2, a cooling coil 26 is wound around the outer periphery of the upper wall surface 21 and is connected to the refrigerant outlet 24 and the refrigerant inlet 25. It is covered by 28. In Figure 3, culture tank 1
A refrigerant jetting header 29 is provided at the upper part of the refrigerant pipe 30 to jet the refrigerant from the refrigerant pipe 30 onto the upper wall surface 21 . As the refrigerant in the refrigerant pipe 30, in addition to a dedicated refrigerant, a culture solution or an upper gas may be cooled and circulated from the circulation system 31 by a cooling device 32, as shown by the two-dot chain line. Figures 1 to 3
Although the coil 7 in the culture solution 2 is not shown in the figure, this may or may not be present. M is stirrer 1
The motor that rotates 0 is shown. Other configurations are 4th
It is similar to the figure.
上記の培養装置において糸状菌の培養を行う方
法は、従来と同様に培養槽１に培養液２を仕込む
とともに、糸状菌を接種し、攪拌機１０を回転さ
せて培養液２を攪拌し、ジヤケツト４および（ま
たは）コイル７に冷媒または熱媒を導入して培養
液２を冷却または加温して培養適温に維持する。
同時に第１図においては冷媒入口２５から冷却用
ジヤケツト２３に冷媒を供給し、冷媒出口２４か
ら排出して上部壁面２１を冷却し、これにより培
養適温より低い温度に維持する。第２図では同様
に冷媒入口２５から冷却用コイル２６に冷媒を通
して上部壁面２１を冷却するが、保冷材２８によ
り保冷を行い、冷却効果を高める。また第３図で
は冷媒管３０の冷媒を冷媒噴出ヘツダ２９から噴
出させて上部壁面２１を冷却する。冷媒として培
養液または上部のガスを利用する場合は循環系３
１から冷却装置３２を通して冷却し循環する。 The method for culturing filamentous fungi in the above-mentioned culture device is to charge the culture solution 2 into the culture tank 1 as in the conventional method, inoculate the filamentous fungi, rotate the stirrer 10 to stir the culture solution 2, and add the culture solution 2 to the jacket 4. And/or a coolant or a heat medium is introduced into the coil 7 to cool or heat the culture solution 2 and maintain it at an appropriate culture temperature.
At the same time, in FIG. 1, a refrigerant is supplied to the cooling jacket 23 from the refrigerant inlet 25 and discharged from the refrigerant outlet 24 to cool the upper wall surface 21, thereby maintaining the temperature lower than the optimum culture temperature. In FIG. 2, the upper wall surface 21 is similarly cooled by passing the refrigerant from the refrigerant inlet 25 to the cooling coil 26, but the cooling effect is enhanced by retaining the coolant using the cold insulating material 28. Further, in FIG. 3, the refrigerant in the refrigerant pipe 30 is jetted out from the refrigerant jetting header 29 to cool the upper wall surface 21. Circulation system 3 when using culture medium or upper gas as a refrigerant
1 and then cooled and circulated through a cooling device 32.
こうして培養液を培養糸状菌の培養適温に維持
し、培養液の液面より上部の壁面を培養適温より
低い温度に維持して糸状菌の培養を行うと、攪拌
等により上部壁面２１に菌体が付着しても、壁面
が培養適温より低い温度に維持されているので、
上部壁面２１における糸状菌の増殖はなく、菌塊
は付着しない。一方、培養液２は培養適温に維持
されているため、培養液中で糸状菌が増殖し、効
率のよい培養が行われる。 In this way, the culture solution is maintained at an appropriate temperature for culturing filamentous fungi, and the wall surface above the liquid level of the culture solution is maintained at a temperature lower than the optimum culture temperature to culture the filamentous fungi. Even if it adheres, the wall surface is maintained at a temperature lower than the optimum culture temperature.
There is no growth of filamentous fungi on the upper wall surface 21, and no bacterial mass is attached. On the other hand, since the culture solution 2 is maintained at a suitable temperature for culture, filamentous fungi grow in the culture solution and efficient culture is performed.
上記のように菌塊の壁面付着を防止することに
より、基本的には増殖した菌体がすべて液内に存
在することになるため、菌体の増殖速度が速くな
るとともに、生産物の生成速度が速くなり、また
培養条件の制御が容易となり、培養の再現性も高
くなる。 By preventing the bacterial mass from adhering to the wall as described above, basically all the proliferated bacterial cells will be present in the liquid, which will increase the growth rate of the bacterial cells and increase the production rate of products. The process becomes faster, the culture conditions are easier to control, and the reproducibility of the culture becomes higher.
試験例
３容の槽内の液面上部の外周壁面に冷却用コ
イル２６を巻付けた培養槽１に、可溶性殿粉1.25
％（ｗ／ｖ）、ペプトン1.29％（ｗ／ｖ）、KH2
PO40.5％（ｗ／ｖ）およびMgSO4・7H2O0.01％
（ｗ／ｖ）を含む培養液（培地）２を投入し、
これを120℃で20分間加熱殺菌後30℃に冷却した
ものに、アスペルギルス・オリゼー1AM2673を
予めフスマ含有培地で培養した培養菌体１ｇを接
種し、次いで培養槽１に配設した冷却用コイル２
６により上部壁面２１を５℃に冷却し、400r.p.
m.で攪拌しつつ、通気量1V.V.M.、培養温度28
℃程度で10日間培養を行つたところ、培養槽の上
部壁面には糸状菌の菌糸状菌塊は肉眼的に全く認
められなかつた（培養液中の菌糸体量；15.0ｇ／
−培養液）。Test example: 1.25 ml of soluble starch was placed in a culture tank 1 with a cooling coil 26 wrapped around the outer peripheral wall above the liquid level in a 3-volume tank.
% (w/v), peptone 1.29% (w/v), KH 2
PO 4 0.5% (w/v) and MgSO 4 7H 2 O 0.01%
(w/v) of culture solution (medium) 2 containing
After heating and sterilizing this at 120°C for 20 minutes and cooling it to 30°C, 1 g of cultured cells of Aspergillus oryzae 1AM2673 cultured in a bran-containing medium was inoculated, and then cooling coil 2 placed in culture tank 1
6, the upper wall surface 21 was cooled to 5°C, and the temperature was increased to 400 r.p.
While stirring at m., aeration volume 1V.VM, culture temperature 28.
When the culture was carried out for 10 days at a temperature of approximately
- culture solution).
なお、培養槽の上部壁面２１を冷却しない場合
は、培養槽１の上部壁面に著しく糸状菌の菌糸状
菌塊の生成が見られ、その付着量は20.0ｇ／−
培養液（乾燥重量）であつた（培養液中の菌糸体
量；2.5ｇ／−培養液）。 In addition, when the upper wall surface 21 of the culture tank 1 is not cooled, the formation of a mycelial mass of filamentous fungi is observed on the upper wall surface of the culture tank 1, and the amount of adhesion is 20.0 g/-.
It was a culture solution (dry weight) (amount of mycelium in the culture solution: 2.5 g/-culture solution).
以上の結果より、培養槽に上部壁面を冷却する
ことにより、菌塊の付着が防止されることがわか
る。 From the above results, it can be seen that by cooling the upper wall surface of the culture tank, adhesion of bacterial mass can be prevented.
なお、上記説明において、培養液を培養適温に
維持する装置および上部壁面を培養適温より低い
温度に維持する装置は図示のものに限らず、他の
類似の手段であつてもよい。また本発明は培養さ
れた糸状菌を目的物とする場合、および糸状菌の
生産物を目的物とする場合、ならびに両者を目的
物とする場合のいずれの場合にも適用できる。 In the above description, the device for maintaining the culture solution at an appropriate culture temperature and the device for maintaining the upper wall surface at a temperature lower than the appropriate culture temperature are not limited to those shown in the drawings, and may be other similar means. Furthermore, the present invention can be applied to cases in which a cultivated filamentous fungus is used as an object, a product of the filamentous fungus is used as an object, and both cases are used as an object.
〔発明の効果〕
本発明によれば、培養槽内の培養液の液面より
上部の壁面を前記糸状菌の培養適温より低い温度
に維持して培養するようにしたので、培養槽の壁
面への菌塊の付着を防止し、効率よく糸状菌の培
養を行うことができる。[Effects of the Invention] According to the present invention, since the wall surface above the liquid level of the culture solution in the culture tank is maintained at a temperature lower than the optimum culture temperature for the filamentous fungi, the wall surface of the culture tank is cultured. It is possible to prevent the adhesion of bacterial masses and to efficiently culture filamentous bacteria.
第１図ないし第３図は本発明の別の実施例によ
る培養装置を示す断面図、第４図は従来の培養装
置を示す断面図である。
各図中、同一符号は同一または相当部分を示
し、１は培養槽、２は培養液、４はジヤケツト、
２１は上部壁面、２３は冷却用ジヤケツト、２６
は冷却用コイル、２８は保冷材、２９は冷媒噴出
ヘツダである。
1 to 3 are sectional views showing a culture device according to another embodiment of the present invention, and FIG. 4 is a sectional view showing a conventional culture device. In each figure, the same reference numerals indicate the same or corresponding parts, 1 is a culture tank, 2 is a culture solution, 4 is a jacket,
21 is an upper wall surface, 23 is a cooling jacket, 26
28 is a cooling coil, 28 is a cold insulator, and 29 is a refrigerant jetting header.
Claims (1)
培養槽内の培養液の液面より上部の壁面を前記糸
状菌の培養適温より低い温度に維持して培養する
ことを特徴とする糸状菌の培養方法。 ２ 液面より上部の壁面の温度が15℃以下である
特許請求の範囲第１項記載の方法。 ３ 糸状菌を培養する培養槽と、この培養槽内の
培養液を前記糸状菌の培養適温に維持する装置
と、前記培養槽の培養液の液面より上部の壁面を
前記糸状菌の培養適温より低い温度に維持する装
置とを有することを特徴とする糸状菌の培養装
置。 ４ 培養適温に維持する装置が冷却もしくは加温
用のジヤケツトまたはコイルである特許請求の範
囲第３項記載の装置。 ５ 培養適温より低い温度に維持する装置が冷却
用ジヤケツト、冷却用コイルもしくは冷媒噴出ヘ
ツダである特許請求の範囲第３項または第４項記
載の装置。[Claims] 1. A method for culturing filamentous fungi in a culture tank,
A method for culturing filamentous fungi, which comprises culturing while maintaining a wall surface above the liquid level of a culture solution in a culture tank at a temperature lower than the optimum culture temperature for the filamentous fungi. 2. The method according to claim 1, wherein the temperature of the wall surface above the liquid level is 15° C. or less. 3. A culture tank for culturing filamentous fungi, a device for maintaining the culture solution in this culture tank at an appropriate temperature for culturing the filamentous fungi, and a wall surface above the liquid level of the culture solution in the culture tank for maintaining the temperature suitable for culturing the filamentous fungi. 1. A culturing device for filamentous fungi, comprising a device for maintaining the temperature at a lower temperature. 4. The device according to claim 3, wherein the device for maintaining the culture at an appropriate temperature is a jacket or coil for cooling or heating. 5. The device according to claim 3 or 4, wherein the device for maintaining the temperature lower than the optimum culture temperature is a cooling jacket, a cooling coil, or a refrigerant jetting header.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23775186A JPS6391075A (en) | 1986-10-06 | 1986-10-06 | Cultivation of mold and device therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23775186A JPS6391075A (en) | 1986-10-06 | 1986-10-06 | Cultivation of mold and device therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6391075A JPS6391075A (en) | 1988-04-21 |
| JPH0548113B2 true JPH0548113B2 (en) | 1993-07-20 |
Family
ID=17019929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23775186A Granted JPS6391075A (en) | 1986-10-06 | 1986-10-06 | Cultivation of mold and device therefor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6391075A (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100374387B1 (en) * | 2000-04-26 | 2003-03-04 | (주)베스트코리아 | Incubator Chiller |
| JP2001299327A (en) * | 2000-04-28 | 2001-10-30 | Mitsubishi Chemical Engineering Corp | Method for controlling temperature of batch-wise fermentation plant |
| WO2005068608A1 (en) * | 2004-01-14 | 2005-07-28 | Daikin Industries, Ltd. | Temperature control device |
| JP4571055B2 (en) * | 2005-09-30 | 2010-10-27 | 学校法人東京農業大学 | Fermentation distillation drying system |
| JP5520495B2 (en) * | 2009-02-12 | 2014-06-11 | 特定非営利活動法人広島循環型社会推進機構 | Method and apparatus for producing ethanol from food waste |
| CN102533541B (en) * | 2010-12-20 | 2015-04-15 | 新奥科技发展有限公司 | Microorganism culture system |
| JP6590494B2 (en) * | 2015-03-12 | 2019-10-16 | 日立造船株式会社 | Waste-derived biomass reactor |
| ES2648968A1 (en) * | 2016-07-05 | 2018-01-09 | Inbiolev, S.L. | Biorreactor for the multiplication of yeasts and lactic bacteria (Machine-translation by Google Translate, not legally binding) |
| CN106492729A (en) * | 2016-11-25 | 2017-03-15 | 江苏鼎弘环境科技有限公司 | A kind of stirred reactor |
| US11629322B2 (en) | 2016-12-05 | 2023-04-18 | Global Life Sciences Solutions Usa Llc | Impeller including one or more turbulators, for a bioreactor system |
-
1986
- 1986-10-06 JP JP23775186A patent/JPS6391075A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6391075A (en) | 1988-04-21 |
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