CN208200973U - A kind of production equipment of serialization supply logarithmic growth phase bacterium solution - Google Patents

A kind of production equipment of serialization supply logarithmic growth phase bacterium solution Download PDF

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Publication number
CN208200973U
CN208200973U CN201820256903.XU CN201820256903U CN208200973U CN 208200973 U CN208200973 U CN 208200973U CN 201820256903 U CN201820256903 U CN 201820256903U CN 208200973 U CN208200973 U CN 208200973U
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China
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bacterium solution
tank
component
growth phase
logarithmic growth
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CN201820256903.XU
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Inventor
刘正初
苏维利
李云峰
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Sun Wu Tianzhi Grass Biological New Materials Co., Ltd.
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Daqing Heavenly Grass Biological New Mstar Technology Ltd
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Abstract

The utility model discloses a kind of production equipment of serialization supply logarithmic growth phase bacterium solution, seeding tank fills liquid amplification culture medium, for receiving and cultivating the activation culture bacterium solution of bacterium liquid activation bottle culture to obtain the seeding tank amplification bacterium solution for being in logarithmic growth phase;Fermentor fills liquid state fermentation culture medium, and the seeding tank for receiving and cultivating seed tank culture expands bacterium solution to obtain the fermentor amplification bacterium solution for being in logarithmic growth phase;Buffer-stored tank for receiving the fermentor amplification bacterium solution of fermentation tank culture in the state of emptying and continuing to carry out deep layer liquid state fermentation, while exporting fermentor amplification bacterium solution;Mixture inoculation tank, the amplification bacterium solution for receiving the conveying of buffer-stored tank is diluted it and is deployed into the equally distributed bacteria suspension of thallus, and exports the bacteria suspension.The technical issues of the utility model solves the problems, such as is to overcome existing microculture or fermentation pattern either bacterium solution without interruption or cannot not can guarantee strain function.

Description

A kind of production equipment of serialization supply logarithmic growth phase bacterium solution
Technical field
The utility model relates to flaxen fiber production fields.Specially a kind of production of serialization supply logarithmic growth phase bacterium solution Equipment.
Background technique
In industrial (medicine, food, beverage, the energy, chemistry etc.) and agricultural (molecule manipulation breeding, biological nitrogen fixation, biological agriculture Medicine, feed) in production and in field of environment protection, it usually needs microorganism is cultivated or sent out using suitable method Ferment.According to operating method, people are divided into microculture or fermentation " in batches " and " continuous " both of which.The former is will to cultivate Base is disposably packed into culture (fermentation) tank, carry out under optimum conditions be inoculated with and by certain time culture (fermentation) after, take The production model of (fermentation) product is all cultivated out.After the latter is inoculation, culture medium is on the one hand continuously fed to culture (hair Ferment) in tank, so that microorganism growth process is in stable state, condition of culture does not change over time, while continuously taking again A kind of production model of (fermentation) product is cultivated out.
According to the growth rate constant of microorganism, i.e., microorganism is generally grown allusion quotation by the difference of division algebra hourly Type curve rough segmentation is lag phase, logarithmic phase, four periods of stationary phase and decline phase.When microorganism is in a closed system culture When, after microorganism grows certain phase, the ratio speed of growth of microorganism reaches maximum, enters logarithmic growth phase at this time.Right If not inhibiting in number growth period or the factor of restriction micro-organisms growth existing, microbes keep a constant maximum ratio Growth, cell quantity are exponentially incremented by.However the limited time of logarithmic growth phase, generally continue several hours to ten Several hours.For needing continuous provide to be in for the microbial fermentation production process of " logarithmic growth phase " bacterium solution, above-mentioned two All there are some key technique obstacles in kind production model." in batches " mode is not obviously able to satisfy the requirement of " serialization ", " even It is continuous " mode is then in culture (fermentation) tank for a long time due to bacterium solution, the logarithmic growth of microorganism may have been had been subjected at once in confession Phase, therefore, it is difficult to avoid " strain functional deterioration causes to produce unstable ".
Utility model content
The technical issues of the utility model solves is to overcome existing microculture or fermentation pattern or cannot connect Continuous the problem of supplying bacterium solution or not can guarantee strain function, provides a kind of production of serialization supply logarithmic growth phase bacterium solution Equipment.
The production equipment of the serialization supply logarithmic growth phase bacterium solution of the utility model, comprising:
Seeding tank fills liquid amplification culture medium, for receiving and cultivating the activation culture bacterium solution of bacterium liquid activation bottle culture Bacterium solution is expanded to obtain the seeding tank in logarithmic growth phase;
Fermentor fills liquid state fermentation culture medium, and the seeding tank for receiving and cultivating the seed tank culture expands bacterium Liquid is in the fermentor amplification bacterium solution of logarithmic growth phase to obtain;
Buffer-stored tank, the fermentor for receiving the fermentation tank culture in the state of emptying expand bacterium solution and make it Continue deep layer liquid state fermentation, while exporting fermentor amplification bacterium solution;
Mixture inoculation tank is diluted and is deployed into it for receiving the amplification bacterium solution that the buffer-stored tank conveying comes The equally distributed bacteria suspension of thallus, and export the bacteria suspension.
Preferably, the production equipment further includes being used to prepare for expansion needed for multiple batches of production seeding tank amplification bacterium solution Increase the amplification culture medium preparation tank of culture medium, the amplification culture medium preparation tank is connect for the seed with the seeding tank Tank conveys amplification culture medium.The production equipment further includes being used to prepare for hair needed for multiple batches of production fermentor amplification bacterium solution The fermentation medium preparation tank of ferment culture medium, the fermentation medium preparation tank are connect for the fermentation with the fermentor Tank conveys fermentation medium.
Preferably, the amplification culture medium preparation tank and/or fermentation medium preparation tank and/or seeding tank and/or hair Fermentation tank and/or buffer-stored tank include the liner that is surrounded by shell and close the lid of the liner, from bottom part ring around described interior Gallbladder is used to accommodate the electricity of collet, the mixing component on the lid and driving the mixing component rotation of heat insulation liquid Machine, the liner have feed pieces and outlet member, the feed pieces can feed liquor or air inlet, the lid be equipped with it is described The outlet component that liner communicates, the collet have can be used as hot water inlet or cooling water outlet the first liquid communication component, It can be used as the second liquid flow component and ventilation member of hot water outlet or cold water inlet.
Preferably, further include control device, the control device and the motor for stirring the liquid in the liner Connection is to control the opening and closing of motor.The level sensing portion connecting with the control device is equipped with inside the liner Part, charging and outlet member of the control device with the liner are separately connected, according to the Liquid level charging detected The opening and closing of component and outlet member.The lid is equipped with and connect with the control device for detecting the air pressure in liner Detection part, the control device are connect according to the air inflow of the pressure control feed pieces detected with the outlet component With the gas output of outlet component.The temperature detection part connecting with the control device, the control are equipped with inside the liner Also liquid component is separately connected device with the feed liquor component of collet and out, to control the feed liquor according to the data of temperature detection part Component and the out opening and closing of liquid component.
Preferably, further including the spoiler for generating resistance for the flowing to bacterium solution on the inner wall of the liner. It further include the defoaming paddle in the liner, the agitating paddle of the defoaming paddle and the mixing component is fixed on same rotary shaft On, and the position of the defoaming paddle is higher than the agitating paddle.
Preferably, the mixed inoculation tank includes mixed tank body and communicates the reception mixed tank with the mixed tank body The caching tank body for the bacteria suspension that body mixture is formed, the mixture tank body are equipped with bacterium solution and flow into component, dilution inflow component and change Gas component, the mixture tank interior, which is equipped with, makes bacterium solution and the mixed uniformly mixed component of dilution, and the caching tank body is equipped with Detect the level sensing component and outlet member of liquid level.The bacterium solution of mixed tank body flows into component, dilution flows into component, ventilation portion The outlet member and level sensing component of part and mixed component and caching tank body are connect with the control device.
The production equipment of the serialization supply logarithmic growth phase bacterium solution of the utility model compared with prior art, has following The utility model has the advantages that
1, the production equipment of the utility model increases buffer-stored bacterium solution and mixture inoculation bacterium solution, the bacterium solution of buffer-stored It can guarantee it is the bacterium solution in logarithmic growth phase, ready-to-use when needing to use bacterium solution, being inoculated with bacterium solution by mixture can realize The bacterium solution of logarithmic growth phase without interruption.
2, amplification culture medium and fermentation medium are respectively in amplification culture medium preparation tank H set in addition and fermentation medium It completes in preparation tank, no longer occupancy seeding tank and fermentor, so that the preparation and bacterium solution of amplification culture medium and fermentation medium Amplification and culture can carry out simultaneously, to improve the efficiency of bacterium solution production.
3, amplification culture medium preparation tank and/or fermentation medium preparation tank and/or seeding tank and/or fermentor and/or slow The pressure test device that storage tank is provided with air pressure in detectable liner is rushed, pressure test device connect with control device, controls Device can air inlet according to the intake and exhaust component of the pressure control liner received and outlet, to realize the automatic control of liner air pressure System.
4, amplification culture medium preparation tank and/or fermentation medium preparation tank and/or seeding tank and/or fermentor and/or slow The liquid level detection device that storage tank is provided with liquid level in detectable liner is rushed, liquid level detection device connect with control device, controls Device can control the feed pieces and outlet member of liner according to liquid level.
5, amplification culture medium preparation tank and/or fermentation medium preparation tank and/or seeding tank and/or fermentor and/or slow The temperature-detecting device that storage tank is provided with temperature in detectable liner is rushed, temperature-detecting device connect with control device, controls Device can control the feed liquor component and liquid component out of collet according to the temperature of liner.
6, the bacterium solution of mixed tank body flows into component, dilution flows into component, take a breath component and mixed component and caching tank body Outlet member connect with the control device, realize mixture, exhaust and discharging automation control.And outlet member can be According to the setting flow velocity bacterium solution without interruption for completing mixture under the control of control device.
Detailed description of the invention
Fig. 1 is that the structure of the production equipment of the serialization supply logarithmic growth phase bacterium solution of the utility model first embodiment is shown It is intended to.
Fig. 2 is the amplification training that the production equipment of logarithmic growth phase bacterium solution is supplied in the serialization of the utility model first embodiment Support the structural schematic diagram of base preparation tank or fermentation medium preparation tank or seeding tank or fermentor or buffer-stored tank.
Fig. 3 is that the mixture of the production equipment of the serialization supply logarithmic growth phase bacterium solution of the utility model first embodiment connects The structural schematic diagram of kind tank.
Appended drawing reference
A bacterium colony culture apparatus, B bacterium solution select test tube, C bacterium liquid activation bottle, D seeding tank, E fermentor, F buffer-stored tank, G Mixture inoculation tank, H amplification culture medium preparation tank, I fermentation medium preparation tank, J control device;
11 shells, 111 motors, 112 agitating paddles, 113 defoaming paddles, 114 spoilers, 115 level sensing components, 116 hand holes, 117 observation windows, 118 lids, 12 liners, 121 feed pieces, 122 outlet members, 123 outlet components, 124 temperature detection parts, 13 collets, 131 first logical liquid components, 132 ventilation members, 133 second logical liquid components, 14 support members;
21 mixed tank bodies, 211 spoilers, 212 dilutions inflow component, 213 bacterium solutions inflow component, 214 ventilation components, 22 Cache tank body, 221 level sensing components, 222 outlet members, 23 support members.
Specific embodiment
The utility model provides a kind of production equipment of serialization supply logarithmic growth phase bacterium solution, for industry, agricultural, doctor Etc. industries, cultivate microorganism and are fermented, comprising:
Seeding tank fills liquid amplification culture medium, for receiving and cultivating the activation culture bacterium solution of bacterium liquid activation bottle C culture Bacterium solution is expanded to obtain the seeding tank in logarithmic growth phase.
Fermentor E fills liquid state fermentation culture medium for receiving and cultivating the seeding tank of the seed tank culture and expands bacterium Liquid is in the fermentor amplification bacterium solution of logarithmic growth phase to obtain.
Buffer-stored tank F, for receiving the fermentor amplification bacterium solution of the fermentor E culture in the state of emptying and making It continues deep layer liquid state fermentation, while exporting fermentor amplification bacterium solution.
Mixture inoculation tank G is diluted and deploys to it for receiving the amplification bacterium solution that the buffer-stored tank F conveying comes At the equally distributed bacteria suspension of thallus, and export the bacteria suspension.
In addition to above structure, in the present embodiment, production equipment in addition it can include bacterium colony culture apparatus A, for pair The original seed of pure rejuvenation is diluted separation and carries out static culture on solid state flat panel culture medium and obtains meeting intrinsic bacterium colony shape The bacterium colony of state feature description.Bacterium solution selects test tube B, fills liquid selective medium, for receiving and cultivating the bacterium colony training The bacterium colony of device A culture is supported to obtain the selectivity culture bacterium solution for being in logarithmic growth phase.It is living to fill liquid by bacterium liquid activation bottle C Change culture medium, the selectivity culture bacterium solution for receiving and cultivating the bacterium solution selection test tube B is in logarithmic growth phase to obtain Activation culture bacterium solution, in the present embodiment, bacterium liquid activation bottle C use conical flask.
As a preferred option, the production equipment further includes that preparation expands needed for bacterium solution for multiple batches of production seeding tank Cultivate the amplification culture medium preparation tank H of amplification culture medium, the amplification culture medium preparation tank H connect with the seeding tank for The seeding tank conveys amplification culture medium.The volume of the amplification culture medium preparation tank H is at least 2 times of the volume of seeding tank, It can guarantee the ample supply that amplification culture medium is carried out to seeding tank.
The production equipment further includes being used to prepare for fermentation medium needed for multiple batches of production fermentor amplification bacterium solution Fermentation medium preparation tank I, the fermentation medium preparation tank I and the fermentor E connect for defeated to the fermentor E Send fermentation medium.The volume of the fermentation medium preparation tank I is at least 2 times of the volume of fermentor E, it is ensured that hair The ample supply of the progress fermentation medium of fermentation tank E.
As a preferred option, the amplification culture medium preparation tank H and/or fermentation medium preparation tank I and/or seeding tank And/or fermentor E and/or buffer-stored tank F include the liner 12 surrounded by shell 11 and the lid for closing the liner 12 118, collet 13, the mixing component on the lid 118 for being used to accommodate heat insulation liquid around the liner 12 from bottom part ring With the motor 111 for driving the mixing component rotation.Shell 11, liner 12 and collet 13 can be it is integrally formed, can also be with Be it is seperated, in the present embodiment, three be it is integrally formed, on the ground by 14 supported standing of support member.
The outer wall of lid 118 is equipped with hand hole 116, hand hole 116 can be inserted a finger into when moving tank body, in order to exert a force.Lid Transparent observation window 117 is additionally provided on body 118, for observing the inner case of liner.Outlet component 123 is additionally provided on lid, It can be used for adjusting the air pressure in liner 12.The liner 12 has feed pieces 121, outlet member 122, and feed pieces 121 can Feed liquor or air inlet.
The collet 13 has the first liquid communication component 131 that can be used as hot water inlet or cooling water outlet, can be used as The second liquid flow component 133 and ventilation member 132 of hot water outlet or cold water inlet.First liquid communication component 131 is set In the highest point of collet 13, the bottom of collet 13 is arranged in second liquid flow component 133.It is needing to cool down to liner When, cold water is injected into collet from second liquid flow component 133, cold water is flowed out from the first liquid communication component 131, and formation follows Ring.When needing to keep the temperature liner, hot water is injected into collet from the first liquid communication component 131, and hot water is from the second liquid Body flow component 133 forms circulation from outflow.Injecting hot water or when cold water, the gas in collet is from 132 row of ventilation member Out, when emptying water, air enters collet by ventilation member 132.There is the dirt in water after long-time service, in collet 13 unavoidably Dirt residual, can be excluded dirt by second liquid flow component 133.
As a preferred option, the production equipment of the utility model further includes control device J, the control device J and use It connect to control the opening and closing of motor 111 in the motor 111 with mixing component.
The level sensing component 115 connecting with the control device J, the control device J are equipped with inside the liner 12 It is separately connected with the feed pieces 121 and outlet member 122 of the liner 12, according to the Liquid level feed pieces detected 121 and outlet member 122 opening and closing.Preferably closed when liquid level reaches the 65-75% of 12 internal height of liner into Expect component 121.As a preferred option, feed pieces 121 and outlet member 122 all have and can be controlled by control device J Valve (not shown), valve can be equipped with the traffic detection function (not shown) of detection feed rate and discharging flow.
The air pressure detection part connecting with the control device J, the control device J and institute are equipped with inside the liner 12 The feed pieces 121 for stating liner 12 connect, feed pieces 121 can into liquids and gases, control device J also with outlet component 123 connections, according to 123 outlet of air inlet and outlet component of the pressure control feed pieces 121 detected.
The temperature detection part 124 connecting with the control device J, the control device J are equipped with inside the liner 12 It is also connect with the first logical liquid component 131 of collet 13 and the second logical liquid component 133, according to the data of temperature detection part 124 Control the opening and closing of the described first logical liquid component 131 and the second logical liquid component 133.The control device J also with collet Ventilation member 132 connects, and controls the opening and closing of ventilation member 132.
The spoiler 114 that resistance is generated for the flowing to bacterium solution, in the present embodiment, choked flow are equipped in the liner 12 Plate 114 is fixed on the inner wall of liner 12, resistance is generated to the bacterium solution of rotation, so that bacterium solution and dilution be made to be better mixed Together.
Defoaming paddle 113 is additionally provided in the liner 12, the agitating paddle 112 of the defoaming paddle 113 and the mixing component is solid Due in same rotary shaft, and the position of the defoaming paddle 113 is higher than the agitating paddle 112.The defoaming agitation of paddle 113 floats on liquid level The foam of top is broken, to eliminate or reduce foam.
The mixed inoculation tank G includes mixed tank body 21 and communicates the reception mixed tank body 21 with the mixed tank body 21 The caching tank body 22 for the bacterium solution that mixture is formed, mixed tank body 21 with caching tank body 22 be in the present embodiment it is integrated, pass through branch Part 23 supported standing in support part on the ground, and caches the lower section that tank body 22 is located at mixed tank body 21.The mixture tank body 21 is equipped with Bacterium solution flow into component 213, dilution flow into component 212 and ventilation component 214, it is described mixture tank body 21 inside be equipped with make bacterium solution and The mixed uniformly mixed component of dilution.The bacterium solution of mixed tank body 21 flows into component 213, dilution flows into component 212, ventilation portion Part 214 and mixed component are connect with the control device J.
In the present embodiment, the spoiler that resistance is generated to the bacterium solution and dilution of movement is equipped in the mixed tank body 21 211。
It is additionally provided with level sensing component 221 in the caching tank body 22, for detecting the liquid of the bacterium solution in caching tank body 22 The bottom of position, caching tank body 22 is equipped with outlet member 222, and outlet member 222 is under the control of control device J, according to setting The bacterium solution that flow velocity completes mixture is without interruption.
The production equipment of the serialization supply logarithmic growth phase bacterium solution of the utility model is cultivated or is fermented to microorganism, The following steps are included:
(1) colonies typical is cultivated, i.e., separation is diluted to the original seed purificated and rejuvenated, and utilize selective solid state rheology Base carries out static culture to the thallus after dilution separation and obtains satisfactory bacterium colony.During static culture, thallus is kept It is static.
(2) bacterium solution is selected, i.e., the colonies typical that step (1) obtains is transported to the test tube equipped with selective liquid culture medium In at the temperature and frequency of oscillation of setting dynamic cultivation to setting time and obtain in logarithmic growth phase selectivity training Bacteria liquid.
(3) bacterium solution is activated, i.e., the selectivity culture bacterium solution for being in logarithmic growth phase obtained in step (2) is transferred to dress It is in the present embodiment conical flask in the bottle of liquid activation medium, and dynamic under the conditions of liquid measure and oscillation frequency are contained in setting Culture obtains the activation culture bacterium solution for being in logarithmic growth phase to the time of setting.
(4) seeding tank expands bacterium solution, i.e., is transferred to the activation culture bacterium solution in logarithmic growth phase and fills liquid amplification The deep layer liquid of setting time is carried out in the seeding tank D of culture medium and under the ventilatory capacity of setting, tank pressure, temperature and stirring rate Culture expands bacterium solution to obtain the seeding tank in logarithmic growth phase.Wherein, the culture of deep layer liquid is that strain is inoculated into tank In, make somatic cells are free to suspend in liquid medium, and the cultural method for being grown or being fermented.Deep-layer liquid culture It generally requires and is passed through fed oxygen from air and is stirred, be traditional microbial fermentation cultural method.Seeding tank amplification cultivation basigamy Side is the chemically pure reagent of known main component, the liquid selective medium used close to step (2).
(5) fermentor expands bacterium solution, i.e., is transported to the seeding tank amplification bacterium solution in logarithmic growth phase and fills liquid hair The deep layer liquid of setting time is carried out in the fermentor E of ferment culture medium and under the ventilatory capacity of setting, tank pressure, temperature and stirring rate State fermentation expands bacterium solution to obtain the fermentor in logarithmic growth phase and function-stable.Fermentor amplification cultivation based formulas is Not separated natural organic matter (such as bean cake powder), close to fermented material (non-cellulose in crudefiber crop bast).
(6) buffer-stored bacterium solution, i.e., by logarithmic growth phase fermentor amplification bacterium solution be transferred to emptying or close to In the buffer-stored tank F of emptying, bacterium solution in buffer-stored tank F under the ventilatory capacity of setting, tank pressure, temperature and stirring rate after It is continuous to carry out deep layer liquid state fermentation, while fermentor amplification bacterium solution being continuously conveyed to mixture and is inoculated with tank G.
(7) mixture inoculation bacterium solution, i.e., be diluted buffer-stored bacterium solution with the inorganic salt solution of setting and utilize vortex Rotation and retardance hit the method combined and are deployed into the equally distributed bacteria suspension of thallus, and make the bacteria suspension according to setting Flow velocity is successively flowed out to supply subsequent inoculations process.
By the step of by increasing buffer-stored bacterium solution and mixture inoculation bacterium solution, the bacterium solution of buffer-stored, which can guarantee, is Bacterium solution in logarithmic growth phase, it is ready-to-use when needing to use bacterium solution, it can be realized by mixture inoculation bacterium solution without interruption The bacterium solution of logarithmic growth phase.
It is carried out the step of preparing amplification culture medium before step (4), and the preparation of amplification culture medium is in amplification culture medium system It is completed in standby tank H, the amplification culture medium for completing preparation, which is transported in seeding tank D, carries out step (4).It further include in step (5) The step of preparing fermentation medium of preceding progress, and the preparation of fermentation medium is complete in ad hoc fermentation medium preparation tank I At the fermentation medium for completing preparation, which is transported in fermentor E, carries out step (5).
In the prior art, amplification culture medium is prepared in seeding tank, and fermentation medium is prepared in fermentor, In the utility model, amplification culture medium and fermentation medium are respectively in amplification culture medium preparation tank H set in addition and fermentation training It supports in base preparation tank I and completes, no longer occupancy seeding tank and fermentor, so that preparation and the bacterium of amplification culture medium and fermentation medium The amplification and culture of liquid can carry out simultaneously, to improve the efficiency of bacterium solution production.
Sterilization steps may insure living contaminants source of the target strain pure culture in the process not from culture medium and equipment, The step includes that emptying first is used to secondly train to the amplification equipped with nutrient media components to the heat preservation water in inner container insulated collet Support the liner of base preparation tank H and/or fermentation medium preparation tank I and/or seeding tank D and/or fermentor E or the expansion to emptying Increase culture medium preparation tank H and/or fermentation medium preparation tank I and/or seeding tank D and/or fermentor E and/or buffer-stored tank F Liner 12 in be passed through the sterilizing that sterilizing steam carries out setting time and/or temperature.That is amplification culture medium preparation tank H and/or hair Ferment culture medium preparation tank I and/or seeding tank D and/or fermentor E can sterilize in the state of emptying, can also fill Have and sterilize in the state of respective liquid, buffer-stored tank F can only sterilize in the state of emptying.
In the present embodiment, sterilize steam temperature be 101-121 DEG C, set period of time 15-30min.Heat preservation is to setting It drives steam to empty to liner injection aseptic compressed air after fixing time, while cooling down to injection cold water in collet for liner, When the temperature of liner drops to the cultivation temperature of setting, the temperature to maintain liner is kept the temperature to liner.Maintain liner 12 Air pressure >=0.01MPa, to maintain the gnotobasis of liner 12.
In sterilization steps, amplification culture medium preparation tank H and/or fermentation medium preparation tank I and/or seeding tank D is detected And/or the air pressure in the liner 12 of fermentor E and/or buffer-stored tank F and/or mixture inoculation tank G, and controlled according to air pressure The air inflow of the feed pieces 121 of liner 12 and the gas output of outlet component 123.In the present embodiment, feed pieces 121 and go out Gas component 123 is equipped with the valve that can be controlled by control device, preferably, valve is additionally provided with detection charge flow rate and outlet The traffic detection function (not shown) of flow passes through the preferred embodiment, it can be achieved that control to 12 air pressure of liner.
Making the temperature of liner 12 after sterilizing reduces, can be by reducing or manually reducing naturally.In the present embodiment, to Collet around liner 12 is passed through cold water, reduces temperature.When the temperature of liner 12 drops to the cultivation temperature of setting, to amplification Culture medium preparation tank H's and/or fermentation medium preparation tank I and/or seeding tank D and/or fermentor E and/or buffer-stored tank F Liner 12 keeps the temperature the cultivation temperature for making it maintain setting.According to the difference of pot liquid, to the different settable different trainings of tank Temperature is supported, to be most suitable for the growth of microorganism in tank.
Detect amplification culture medium preparation tank H and/or fermentation medium preparation tank I and/or seeding tank D and/or fermentor E And/or in the liner 12 of buffer-stored tank F liquid liquid level, and control according to liquid level the feed pieces 121 of liner 12 and go out Expect the opening and closing of component 122.Preferably feed pieces are closed when liquid level reaches the 65-75% of 12 internal height of liner 121.As a preferred option, feed pieces 121 and outlet member 122 all have the valve that can be controlled by control device and (scheme In be not shown), valve can be equipped with detection feed rate and discharging flow traffic detection function (not shown).
Above embodiments are only the exemplary embodiment of the utility model, are not used in limitation the utility model, this is practical new The protection scope of type is defined by the claims.Those skilled in the art are right in the essence and protection scope of the utility model The various modifications or equivalent replacements that the utility model is made also fall within the protection scope of the present utility model.

Claims (15)

1. a kind of production equipment of serialization supply logarithmic growth phase bacterium solution characterized by comprising
Seeding tank fills liquid amplification culture medium, for receiving and cultivating the activation culture bacterium solution of bacterium liquid activation bottle culture to obtain It obtains the seeding tank in logarithmic growth phase and expands bacterium solution;
Fermentor fills liquid state fermentation culture medium, for receive and cultivate the seed tank culture seeding tank expand bacterium solution with It obtains the fermentor in logarithmic growth phase and expands bacterium solution;
Buffer-stored tank, the fermentor for receiving the fermentation tank culture in the state of emptying expand bacterium solution and continue to Deep layer liquid state fermentation is carried out, while exporting fermentor amplification bacterium solution;
Mixture inoculation tank, the amplification bacterium solution for receiving the buffer-stored tank conveying are diluted it and are deployed into thallus Equally distributed bacteria suspension, and export the bacteria suspension.
2. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 1, which is characterized in that the life Producing equipment further includes the amplification culture medium system being used to prepare for amplification culture medium needed for multiple batches of production seeding tank amplification bacterium solution Standby tank, the amplification culture medium preparation tank are connect for conveying amplification culture medium to the seeding tank with the seeding tank.
3. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 2, which is characterized in that the expansion The volume of increasing culture medium preparation tank is at least 2 times of the volume of seeding tank.
4. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 2, which is characterized in that the life Producing equipment further includes the fermentation medium system being used to prepare for fermentation medium needed for multiple batches of production fermentor amplification bacterium solution Standby tank, the fermentation medium preparation tank are connect for conveying fermentation medium to the fermentor with the fermentor.
5. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 4, which is characterized in that the hair The volume of ferment culture medium preparation tank is at least 2 times of the volume of fermentor.
6. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 1, which is characterized in that the expansion Increase culture medium preparation tank and/or fermentation medium preparation tank and/or seeding tank and/or fermentor and/or buffer-stored tank includes The lid of the liner and the closing liner that are surrounded by shell, the folder for being used to accommodate heat insulation liquid around the liner from bottom part ring The motor of set, the mixing component on the lid and the driving mixing component rotation, the liner have feed pieces And outlet member, the feed pieces can feed liquor or air inlet, the lid is equipped with the outlet component that communicates with the liner, described Collet has the first liquid communication component that can be used as hot water inlet or cooling water outlet, can be used as hot water outlet or cold water enters The second liquid flow component and ventilation member of mouth.
7. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 6, which is characterized in that further include Control device, the control device connect with the motor for stirring liquid in the liner thus control motor unlatching and It closes.
8. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 7, which is characterized in that in described The level sensing component connecting with the control device, the charging and discharging of the control device and the liner are equipped with inside gallbladder Component is separately connected, according to the opening and closing of the Liquid level feed pieces and outlet member detected.
9. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 7, which is characterized in that the lid Body, which is equipped with, to be connect for detecting the air pressure detection part in liner, the control device and the outlet portion with the control device Part connection is according to the gas output of the air inflow of the pressure control feed pieces detected and outlet component.
10. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 7, which is characterized in that described Be equipped with the temperature detection part connecting with the control device inside liner, the control device also with the feed liquor component of collet and Liquid component is separately connected out, controls the feed liquor component with the data according to temperature detection part and liquid component beats on and off out It closes.
11. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 6, which is characterized in that also wrap Include the spoiler for generating resistance for the flowing to bacterium solution on the inner wall of the liner.
12. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 6, which is characterized in that also wrap The defoaming paddle being located in the liner is included, the agitating paddle of the defoaming paddle and the mixing component is fixed in same rotary shaft, And the position of the defoaming paddle is higher than the agitating paddle.
13. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 7, which is characterized in that described Mixture inoculation tank includes mixed tank body and communicates the bacteria suspension that the reception mixed tank body mixture is formed with the mixed tank body Tank body is cached, the mixture tank body is equipped with bacterium solution inflow component, dilution flows into component and ventilation component, in the mixture tank body Portion, which is equipped with, makes bacterium solution and the mixed uniformly mixed component of dilution, and the caching tank body is equipped with the level sensing component of detection liquid level And outlet member.
14. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 13, which is characterized in that mixture The bacterium solution of tank body flows into component, dilution flows into component, the outlet member and liquid of ventilation component and mixed component and caching tank body Position detection part is connect with the control device.
15. the production equipment of serialization supply logarithmic growth phase bacterium solution according to claim 13, which is characterized in that described The spoiler that resistance is generated to the bacterium solution and dilution of movement is equipped in mixed tank body.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112481114A (en) * 2020-11-30 2021-03-12 中商构能生态科技(天津)有限公司 Liquid fermentation system for preparing broussonetia papyrifera fermentation liquor and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112481114A (en) * 2020-11-30 2021-03-12 中商构能生态科技(天津)有限公司 Liquid fermentation system for preparing broussonetia papyrifera fermentation liquor and application thereof

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