JPH0534945B2 - - Google Patents
Info
- Publication number
- JPH0534945B2 JPH0534945B2 JP22699185A JP22699185A JPH0534945B2 JP H0534945 B2 JPH0534945 B2 JP H0534945B2 JP 22699185 A JP22699185 A JP 22699185A JP 22699185 A JP22699185 A JP 22699185A JP H0534945 B2 JPH0534945 B2 JP H0534945B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- carrier particles
- container
- liquid medium
- adhesion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002245 particle Substances 0.000 claims description 98
- 239000002609 medium Substances 0.000 claims description 51
- 239000007788 liquid Substances 0.000 claims description 48
- 230000001419 dependent effect Effects 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 102
- 238000000034 method Methods 0.000 description 11
- 230000005484 gravity Effects 0.000 description 9
- 230000021164 cell adhesion Effects 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 241000699802 Cricetulus griseus Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 229910000976 Electrical steel Inorganic materials 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 229910001209 Low-carbon steel Inorganic materials 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 208000007913 Pituitary Neoplasms Diseases 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000002495 Uterine Neoplasms Diseases 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 239000006247 magnetic powder Substances 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 208000010916 pituitary tumor Diseases 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 206010046766 uterine cancer Diseases 0.000 description 1
- 229910000859 α-Fe Inorganic materials 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22699185A JPS6287092A (ja) | 1985-10-14 | 1985-10-14 | 接着依存性細胞の担体粒子への接着方法および装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22699185A JPS6287092A (ja) | 1985-10-14 | 1985-10-14 | 接着依存性細胞の担体粒子への接着方法および装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6287092A JPS6287092A (ja) | 1987-04-21 |
| JPH0534945B2 true JPH0534945B2 (fr) | 1993-05-25 |
Family
ID=16853795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22699185A Granted JPS6287092A (ja) | 1985-10-14 | 1985-10-14 | 接着依存性細胞の担体粒子への接着方法および装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6287092A (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11981884B2 (en) | 2022-10-17 | 2024-05-14 | Upside Foods, Inc. | Pipe-based bioreactors for producing comestible meat products and methods of using the same |
-
1985
- 1985-10-14 JP JP22699185A patent/JPS6287092A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6287092A (ja) | 1987-04-21 |
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