JPH0534944B2 - - Google Patents
Info
- Publication number
- JPH0534944B2 JPH0534944B2 JP60180983A JP18098385A JPH0534944B2 JP H0534944 B2 JPH0534944 B2 JP H0534944B2 JP 60180983 A JP60180983 A JP 60180983A JP 18098385 A JP18098385 A JP 18098385A JP H0534944 B2 JPH0534944 B2 JP H0534944B2
- Authority
- JP
- Japan
- Prior art keywords
- liquid medium
- cells
- culture
- container
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims abstract description 103
- 239000002245 particle Substances 0.000 claims description 30
- 230000001419 dependent effect Effects 0.000 claims description 15
- 238000012136 culture method Methods 0.000 claims description 2
- 230000002062 proliferating effect Effects 0.000 claims 2
- 239000006185 dispersion Substances 0.000 abstract description 17
- 230000005484 gravity Effects 0.000 abstract description 12
- 239000002609 medium Substances 0.000 description 99
- 210000004027 cell Anatomy 0.000 description 90
- 241000196324 Embryophyta Species 0.000 description 50
- 238000000034 method Methods 0.000 description 17
- 238000012258 culturing Methods 0.000 description 10
- 210000004748 cultured cell Anatomy 0.000 description 7
- 238000007599 discharging Methods 0.000 description 5
- 238000010008 shearing Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000013543 active substance Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 241001071917 Lithospermum Species 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60180983A JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60180983A JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6244173A JPS6244173A (ja) | 1987-02-26 |
| JPH0534944B2 true JPH0534944B2 (fr) | 1993-05-25 |
Family
ID=16092691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60180983A Granted JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6244173A (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443985A (en) * | 1993-07-22 | 1995-08-22 | Alberta Research Council | Cell culture bioreactor |
| KR100848938B1 (ko) | 2007-04-20 | 2008-07-29 | 코아스템(주) | 세포배양관 및 이를 포함하는 대량 세포배양장치 |
| JP5151923B2 (ja) * | 2008-11-18 | 2013-02-27 | 株式会社Ｉｈｉ | 微生物濃縮装置 |
| JP5589478B2 (ja) * | 2010-03-24 | 2014-09-17 | 株式会社Ｉｈｉ | 微生物濃縮装置 |
| US11981884B2 (en) | 2022-10-17 | 2024-05-14 | Upside Foods, Inc. | Pipe-based bioreactors for producing comestible meat products and methods of using the same |
-
1985
- 1985-08-20 JP JP60180983A patent/JPS6244173A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6244173A (ja) | 1987-02-26 |
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