JPH05310587A - Extract of banaba leaf, its use and antidiabetic agent - Google Patents

Abstract

PURPOSE:To obtain a banaba (Lagerstroemia speciosa) leaf extract having excellent preservability and usable as a drink or food by industrially extracting banaba leaf under prescribed condition and using the extract as freeze-dried powder or as a drink, to provide a method for using the extract and to provide an antidiabetic agent containing the extract as a component. CONSTITUTION:Dried and crushed banaba leaf is extracted with hot water and filtered to obtain a hot-water extract liquid and a residue. The residue is extracted with methanol and filtered to obtain a methanol extract liquid. The hot-water extract and the methanol extract are separately sterilized in an autoclave and freeze-dried to obtain the objective banaba leaf extract powder. The extract powder can be mixed into a food or drink after diluting to a concentration of about 0.1-10%. It is also effective as an antidiabetic agent.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to banaba leaves (Lagerstroemia speciosa) from Southeast Asia including the Philippines.
l.) and a method for using it and an antidiabetic agent.

[0002]

2. Description of the Related Art Banaba is a plant of the family Liliaceae that is distributed in tropical Asia, and is also known as Pleurotus cornucopia. In the Philippines, which is the origin of the plant for a long time, this leaf has been decocted and consumed. On the other hand, however, since Banaba leaves were produced in the tropics, various bacteria were severely contaminated, and a sufficient sterilization operation was necessary for normal drinking. Therefore, in order to extract this main component, a method has been adopted in which banaba leaves are first crushed, then heat sterilized and then directly stuffed into tea bags, etc. It was

[0003]

In such an extraction method for decocting banaba leaves, it is difficult to carry out homogeneous extraction because the extraction conditions are not prepared at all. In addition, the extract once decocted is easy to rot and does not last long, so
It was very troublesome to brew the required amount every day.

Further, although it has been generally known that the above-mentioned medicinal properties for drinking are effective, its antidiabetic active ingredient has not been confirmed, and therefore, it cannot be industrially produced as a highly pure antidiabetic agent. It was difficult.

Therefore, in the present invention, the above-mentioned problems are improved and banaba leaves are industrially extracted under certain conditions,
Furthermore, as a dry powder or a beverage thereof, it has excellent storability, and along with a banaba leaf extract which can be used for food and drink and a method of using the same, clinically evaluates the pharmacological effect of this banaba leaf extract as an antidiabetic agent. It is confirmed that the anti-diabetic agent containing this extract is to be provided.

[0006]

In the present invention, banaba leaves are extracted with hot water or an organic solvent or a mixed solution of an organic solvent and water, and the water-soluble fraction containing the main components and the fat-soluble It succeeded in extracting the fraction, obtaining the objective Banaba leaf extract powder extract, the Banaba leaf drinkable substance, and the antidiabetic agent which uses this Banaba leaf extract powder extract as a component, extracting a fraction and solving the said subject.

The banaba leaf extract powder extract of the present invention is obtained by extracting banaba leaves with hot water at about 60 to 100 ° C., with an organic solvent such as methanol or ethanol, or with a mixed solution of the organic solvent and water. It can be obtained by extraction or by extracting the residue after the hot water extraction with an organic solvent or a mixed solution of an organic solvent and water, concentrating the extracted solution and drying, for example, lyophilizing.

If the above extract solution is concentrated, sterilized by autoclave, and frozen, for example, freeze-dried, a banaba leaf extract powder extract that can be safely consumed can be obtained.

The banana leaf beverage contains about 60 banana leaves.
Extracted with hot water of ℃ ~ 100 ℃, or extracted with organic solvent or a mixed solution of organic solvent and water, or banaba leaves with hot water of about 60 ℃ ~ 100 ℃, the residue is an organic solvent Or, extract it with a mixed solution of organic solvent and water, adjust it to an appropriate concentration, put it in a container, and sterilize it by retort or adjust the pH of this extract to less than 4.6. Even if it is not treated, it can be obtained by sterilization by a method of heating the temperature of the central portion at 85 ° C. for 10 minutes. That is, it can be obtained by a sterilization treatment based on the sterilization conditions defined by the Food Sanitation Law.

The concentration of the banaba leaf extract powder extract to banaba leaf drink obtained by the above hot water extraction is about 0.1.
If it is adjusted to be 10% to 10% and mixed into foods and beverages, it is possible to eat and drink favorably. If it is around 2%, it will be the cheapest to eat and drink.

An anti-ulcer agent can be obtained by using the banaba leaf extract powder extract obtained as described above as a component, and its anti-diabetic effect was confirmed by an animal test using diabetic mice.

[0012]

Industrial Applicability According to the present invention, the extraction method can be adjusted industrially under certain conditions, and it is possible to obtain an extract with high accuracy and safety, which is simpler than the conventional brewing method. Further, the extract is also obtained as an extract by separating it into a water-soluble component and a fat-soluble component.

This banaba leaf extract can be diluted to an appropriate drinkable concentration to be used as a canned beverage, a bottled beverage, etc., and the lyophilized powder of the extracted extract can be preserved as it is, in the form of tablets or capsules. It can be made into granules or mixed with other herbal medicines or juices to make it cheap to drink.

Further, it has been revealed that the component containing the above extract has an excellent antidiabetic effect.

[0015]

【Example】

[Production Method] Fresh leaves of Philippine banaba are dried in an appropriate amount and crushed with a crusher. 475 g of well-mixed banaba leaves are mixed with 15 liters of distilled water at 95 ° C.
It was extracted for a minute and filtered to obtain a hot water extract and a hot water extraction residue. Next, the hot water extraction residue was dehydrated, and then the solution was immersed in 15 liters of methanol at room temperature for 48 hours and filtered to obtain a methanol extract and a methanol extraction residue.

The above hot water extract and methanol extract are
Each was concentrated by an evaporator to obtain a banaba leaf extract extract by hot water extraction and a banaba leaf extract extract by methanol extraction.

Further, this extract was sterilized by a pressure sterilization method at 121 ° C. for 15 minutes in an autoclave whose sterilizing effect was already confirmed, and then freeze-dried to obtain a powder.

At this time, lyophilized powder by hot water extraction,
The recovery rate of freeze-dried powder by methanol extraction is shown below. -Sample recovery (g) Recovery rate (%)-Hot water extract 58.7 12.4-Methanol extract 15.3 3.2-Total 74.0 15.6

In the above examples, when the temperature of hot water extraction was set to less than about 60 ° C., the recovery rate of freeze-dried powder by hot water extraction remarkably decreased. It was confirmed that the same effect was obtained and the components could be extracted by extracting with ethanol instead of methanol. Also, if methanol or ethanol extraction is performed from the beginning, the portion eluted in the water-soluble fraction is also extracted with methanol or ethanol. Therefore, the recovery rate of the methanol or ethanol extract is the above-mentioned hot water extract and methanol extract recovery. Rate added 15
It is estimated to be in the vicinity of%.

A different banaba leaf extract solution is prepared by diluting and dissolving the lyophilized powder obtained by the above method by hot water extraction with water or the like, or by diluting the concentration of the hot water extract. Were packed in a can and sterilized by a commonly used retort sterilization method, for example, by heating at 120 ° C. for 4 minutes or more, and stored in a sealed state.

At this time, the concentration of the banaba leaf extract solution may be appropriately selected depending on its application, but if the concentration exceeds 5%, bitterness is felt, and if it exceeds about 10%, it is generally bitter and difficult to drink. The concentration around 2% was the easiest to drink.

According to the Food Sanitation Law, those having a pH of 4.6 or higher require retort sterilization. However, since retort sterilization may accelerate the deterioration of the contents, PH 4.6 is added by adding a PH adjuster. Below, preferably about PH3.
It can also be obtained by a sterilization treatment based on the sterilization conditions stipulated by the Food Sanitation Law, such as a method of adjusting the temperature to 7 or less and heating the temperature of the central portion at 85 ° C. for 10 minutes without performing the retort sterilization treatment.

From the above, when the banaba leaf extract powder extract or banaba leaf drink is mixed with, for example, calorie-calculated barley food for diabetic patients, the pharmaceutical effect is enhanced by increasing the concentration, but the concentration is 5%. It was found that when the concentration exceeds, the bitterness is strong and it is difficult to drink, and about 2% is the most suitable concentration.

[0024] [antidiabetic activity test] type II hereditary diabetic mice (KKA ^y T ^a J ^c1 mice, 4 weeks old) were first basal diet in order to smooth the environment (casein 25.0%, salt mixture 3.5 %, Vitamin mixture 1.0%, vitamin C and vitamin K 0.2%, choline chloride 0.2%, corn oil 5.0
%, Sugar 49.8%, starch α15.3%) and raised for 1 week (acclimation period),

Subsequently, these were divided into two groups (7 animals per group), one as a control group and the other as a banaba extract group. The control group was continuously administered with a basic diet, and the banaba extract group was administered. As for the basic food, a 3% food of Banaba (3.0% of Banaba leaf extract powder extract, casein 2) was prepared by mixing the hot water extract of the Banaba extract powder extract obtained by the above-mentioned manufacturing method.
5.0%, salt mixture 3.5%, vitamin mixture 1.0%, vitamin C and vitamin K 0.2%, choline chloride 0.2
%, Corn oil 5.0%, sugar 47.4%, starch α1
(4.7%) was administered and the animals were raised for 1 week each,

Subsequently, a basic diet was administered to the control group, and to the banaba extract group, the hot water extract of the banaba extract powder extract obtained by the above-mentioned manufacturing method was mixed with the above basic diet. Banaba 5% food (banaba leaf extract extract 5.0%, casein 25.0%, salt mixture 3.5%, vitamin mixture 1.0%, vitamin C and vitamin K0.2
%, Choline chloride 0.2%, corn oil 5.0%, sugar 4
6.0% and starch α14.1%) were administered and the animals were bred for 3 weeks.

Then, the body weight during feeding, the feed intake, and the water intake were measured, and after starting the test (after the acclimatization period), tail vein blood was collected every week to quantify the blood glucose level. The results are shown in Table 1. The statistical processing of the results is
Student-t test was used.

[0028]

[Table 1]

From Table 1, regarding the amount of weight gain, both groups showed the largest growth in the first week, and the amount of increase thereafter tended to be small, and there was no difference between the two groups. There was almost no difference in water consumption between the two groups. However, as to the blood glucose level, as shown in FIG. 1, the control group had a high level from the first week and reached a substantially flat state (plateau) at the third week,
On the other hand, in the Banaba group, the low value was clearly maintained, there was almost no change during the first to third weeks, and it was slightly higher at the fourth week.

Therefore, when the diet containing the Banaba extract powder extract according to the present invention is administered from the early stage of the onset of diabetes, it is revealed that the elevation of the blood glucose level is suppressed and the progression of diabetes is suppressed, and the Banaba diet to be administered. It was also clarified that even if the concentration was 3%, the blood sugar level suppressing effect was sufficiently exerted. Furthermore, it can be inferred that the concentration is lower than that.

Further, since the total feed intake of the Banaba extract group for 4 weeks after the acclimation period was 114.2 g, it means that an average of about 0.58 g per animal was ingested per day.
% Dietary intake of Banaba leaf extract is about 0.0
17 g / day, about 0.029 g / in case of administration of 5% Banaba food
It turned out to be a day.

On the other hand, since the average body weight of the mice in the banaba extract group during the test period was about 24.7 g, the average feed intake per kg of body weight was about 0.
69 / day · kg, about 1.17 g when administered with Banaba 5% diet
/ Day · kg was found.

In general, since it is usually taken three times a day,
The intake amount is 1/3 of that, and it is about 0.23 g per 1 kg body weight in the case of administration of 3% Banaba food, and this case is the minimum amount used in this study.
It can be estimated that 0.23 g or less of Banaba extract per g is also effective.

[Brief description of drawings]

FIG. 1 is a graph showing blood glucose levels of mice during a test period.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Hisanao Takeuchi 2783-34 Otani, Shizuoka City, Shizuoka Prefecture

Claims (8)

[Claims] 1. A banaba leaf extract powder extract obtained by extracting banaba leaves with hot water at about 60 ° C. to 100 ° C., concentrating the extract solution and drying. 2. A banaba leaf extract powder extract obtained by extracting banaba leaves with an organic solvent or a mixed solution of an organic solvent and water, concentrating the extract solution and drying. 3. Banaba leaves are extracted with hot water of about 60 ° C. to 100 ° C., the residue is extracted with an organic solvent or a mixed solution of an organic solvent and water, and the extracted solution is concentrated and dried to obtain Banaba leaf extract powder extract. 4. The banaba leaf extract powder extract according to any one of claims 1 to 3, which is obtained by concentrating the extract solution, sterilizing by autoclaving, and drying this. 5. Banaba leaves are extracted with hot water of about 60 ° C. to 100 ° C. or an organic solvent, or a mixed solution of an organic solvent and water, or banaba leaves are extracted with hot water of about 60 ° C. to 100 ° C. Then, the residue is extracted with an organic solvent or a mixed solution of an organic solvent and water, adjusted to an appropriate concentration, packed in a container, and sterilized under the sterilization conditions based on the Food Sanitation Act. Banaba leaf drink. 6. About 0.1 to 10% of the banaba leaf extract powder extract or banaba leaf drink according to any one of claims 1 to 5.
A method for using a food and drink containing a banaba leaf extract, which comprises adjusting the concentration before and after the mixture and mixing it in foods and beverages. 7. Banaba leaves are extracted with hot water of about 60 ° C. to 100 ° C., or an organic solvent, or a mixed solution of an organic solvent and water, or banaba leaves are extracted with hot water of about 60 ° C. to 100 ° C. Then, the residue is extracted with an organic solvent or a mixed solution of an organic solvent and water, and the extract solution is concentrated and dried to obtain an antidiabetic agent containing a banaba leaf extract powder extract as a component. 8. The antibacterial agent according to claim 7, which comprises the banaba leaf extract powder extract according to any one of claims 1 to 3 obtained by concentrating the extract solution, sterilizing by autoclaving, and drying the solution. Diabetic agent.