JPH0525032A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having skin-beautifying effect to prevent the chapped skin, keep the moisture of the skin and make moist skin by using phosvitin which is a main component of yolk protein contained in the avian yolk granule or restricted hydrolyzate of phosvitin as an active component. CONSTITUTION:The objective cosmetic contains phosvitin or its restricted hydrolyzate (preferably having a molecular weight of >=1,000). Phosvitin is a characteristic phosphorylated glycoprotein constituting the main component of yolk protein present in yolk granule, having a molecular weight of about 3,000-40,000, containing serine as about half of the amino acid residues wherein almost all serine units being bonded to phosphoric acid through ester bond and having sugar chain. The amount of the active component is preferably 0.001-10wt.% based on the total cosmetic. The cosmetic has remarkably excellent skin-beautifying effect such as improvement of chapped skin, loss of luster, wrinkles, etc., and the application of moisture by the retension of moisture in the skin. It can be applied without giving undesirable feeling to the skin.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to cosmetics, and more specifically, it prevents or improves rough skin by improving the state of keratin,
In addition, it is intended to provide a cosmetic having a beautiful skin effect of keeping skin moisture and moisturizing the skin by improving the condition caused by dryness of the skin.

[0002]

2. Description of the Related Art Generally, skin dryness occurs when the amount of sebum secretion decreases and the water barrier function of the stratum corneum decreases, resulting in increased transepidermal water loss (hereinafter abbreviated as TEWL). Therefore, when the skin dryness is exacerbated in winter, due to excessive skin washing, decrease in skin secretions due to age, constitution, etc., and when the water content of the stratum corneum decreases to about 10% or less (called dry skin), Multiple peeling is likely to occur, the gloss of the skin deteriorates, fine wrinkles become noticeable, and makeup is deteriorated. As a result, a phenomenon of rough skin occurs.

[0003] Conventionally, in order to improve the condition of the stratum corneum of the skin, it is necessary to prevent a decrease in the moisture content of the stratum corneum and maintain normal skin function, and various methods have been studied so far. Came. As a result, the proposed method is:
A method of suppressing TEWL by using a blocking agent such as petrolatum ointment or water-in-oil emulsion that has good adhesion to the skin and is hydrophobic, and has a hygroscopic and moisturizing ability such as sorbitol, ethylene glycol, glycerin, etc. Improves skin hydration effect by blending sugar alcohols or polyhydric alcohols, proteins such as casein and collagen (hydrolyzate), and moisturizers such as organic acid salts such as sodium pyrrolidonecarboxylate and sodium lactate Another method was to add placenta extract, vitamins, hormones, etc. to promote metabolic activation and repair keratin.

[0004]

However, none of the above-mentioned conventionally known methods has sufficient skin water-retaining ability and has a small effect of improving keratin, and when an occlusive agent is used, oil is not used. There is a drawback that it gives an unpleasant feeling such as sticky, and on the other hand, even if a moisturizing agent is used, a large amount must be added in order to enhance the effect, and as a result, a sticky feeling or slimy feeling etc. There was a problem of giving an unpleasant feeling. Furthermore, when an extract of animal tissue such as placenta, vitamins, hormones, etc. are used, there are problems in safety related to side effects and stability related to time.

The present invention has been made in view of such circumstances, and improves the condition of the stratum corneum of the skin caused by dryness such as rough skin, lack of gloss and fine wrinkles, and moisturizes by retaining sufficient skin moisture. It is an object of the present invention to provide a cosmetic that has a so-called beautiful skin effect of improving or preventing rough skin and adjusting texture, and that has almost no problems in safety, stability and feel.

[0006]

Means for Solving the Problems As a result of intensive studies to solve the above-mentioned problems, the present inventor has found that phosvitin has a high skin water-retaining function-promoting action and keratin improving effect, and has an excellent skin-beautifying effect. The present invention has been found out, and based on this, the present invention has been completed.

That is, the present invention is a cosmetic characterized by containing phosvitin and / or a limited hydrolyzate of phosvitin, and in a preferred embodiment, the content of phosvitin and / or a limited hydrolyzate of phosvitin is To 0.001 to 10% by weight based on the total amount of the cosmetic.

The present invention will be described in detail below. Phosvitin applied to the present invention, which is also called phosphovitin or fosvitin, is the main component of the yolk protein contained in the yolk granules of vertebrates, especially birds, and has a molecular weight of about 3
About 10,000 to 40,000, about half of the amino acid residues are serine, most of which are ester-bonded to phosphoric acid, and a characteristic phosphorylated glycoprotein having a sugar chain. Further, in the present invention, phosvitin may be used in the form of a hydrolyzate in a molecular weight range capable of maintaining its properties, preferably a molecular weight of 1,000 or more.

As a method for obtaining such phosvitin, for example, J. Am. Chem. Soc. Volume 71 3670-3
679 (1949), J. Agric. Food. C
hem. 25, pp. 632-637 (1977) or JP-A-3-56500, a method of separating from egg yolk and the like can be mentioned. A crude extract containing phosvitin may be used depending on the extraction state of phosvitin from egg yolk, and in this case, the content of phosvitin in the crude extract is preferably in the range of 30 to 100%. On the other hand, as a method for obtaining the limited hydrolyzate of phosvitin, an ordinary method may be used, for example, phosvitin may be limitedly decomposed by a proteolytic enzyme and then fractionated.

The phosvitin thus obtained is
It is a white powder with an odorless luster and is hardly soluble or insoluble in various organic solvents such as methanol and acetone, but it dissolves well in water and can be easily blended with various cosmetic bases. It has advantages. Furthermore, this substance does not cause discoloration, odor, decomposition and inactivation over time when formulated into various preparations, and there is no concern about human toxicity or skin damage.
It can be used safely. Further, it is known that phosvitin has heat stability despite being a protein and has no antigenicity, which is an advantage when incorporated into a cosmetic.

In the present invention, the content of phosvitin or the limited hydrolyzate of phosvitin is usually 0.001 to 10% by weight, preferably 0.0
It is 1 to 1% by weight. If the amount is less than 0.001% by weight, the skin-beautifying effect is not sufficiently obtained, and if the amount exceeds 10% by weight, the effect is not enhanced and it is uneconomical. As a method of blending, it is preferable to dissolve phosvitin in the aqueous phase component and then mix it with the oil phase component.

Further, as the cosmetic base of the present invention, any base usually used in cosmetics can be used. Although not specifically specified, specific examples include creams, emulsions, oils, lotions, packs, ointments, and the like, and creams, emulsions, oils and the like are particularly preferable from the viewpoint of transdermal absorbability. As the main ingredients used, in creams, emulsions, oils and ointment bases,
There are beeswax, cetanol, olive oil, stearic acid, lanolin, petrolatum, liquid paraffin, glycerin, propylene glycol monostearate, POE cetyl ether, etc., and for lotion bases, oleyl alcohol, ethanol, glycerin, 1,3 -Butylene glycol, POE lauryl ether, sorbitan monolaurate, etc. Furthermore, examples of the pack agent include polyvinyl acetate emulsion, polyvinyl alcohol, ethanol and the like. From these raw materials, appropriate ones are appropriately selected according to the intended dosage form, and are prepared into cosmetics such as creams, emulsions, oils, lotions, packs and ointments by a conventional method.

To describe the features of the present invention, as described above, casein, which is one of the phosphoproteins similar to phosvitin of the present invention, has a moisturizing action, an emulsifying action, etc., and cream, milk, etc. However, phosvitin has a significantly better skin water retention function than the above-mentioned substances, although the skin water retention ability and stratum corneum improving effect were still not sufficient. It was discovered that it has a stimulating effect on keratin and an effect of improving the stratum corneum, and is intended to be applied to cosmetics.

Next, in order to demonstrate how the cosmetics of the present invention are excellent in terms of skin-beautifying effect, Examples 2 and 3 described below are used.
Using the milky lotion and hand cream shown in, the actual use test was carried out to confirm the efficacy. As comparative products, phosvitin in the emulsion of Example 2 described later was replaced with casein (comparative product 1) and placenta extract (commercial placenta extract) prepared emulsion (comparative product 2) and The hand cream prepared by replacing the phosvitin in the hand cream of Example 3 with casein (comparative product 3) and the placenta extract (commercial placenta extract) were used. The test method is as shown below. (Test method) 150 Japanese adult women with dry skin,
Twenty-five individuals each were randomly divided into 6 groups (A to F groups). On the face of group A, the emulsion of the present invention, on the face of group B, the emulsion of comparative product 1, and on the face of group C, the emulsion of comparative product 2,
In addition, the hand cream of the present invention is applied to the fingers of the D group by E
The hand cream of Comparative product 3 was applied to the fingers of the group, and the hand cream of Comparative product 4 was applied to the fingers of the F group for 4 weeks. After 4 weeks, the groups were compared for the improvement status of various evaluation factors and the status of spontaneous deterioration. For the evaluation of the rough skin condition, the stratum corneum was isolated from the facial skin by a tape stripping method, and the stratum corneum was determined to have a degree of multilayer peeling by observing the stratum corneum with an optical microscope according to the method described in JP-A-63-113358. The presence or absence of nuclear cells was evaluated on a 5-point scale. The results are shown in Table 1, Table 2 and Table 3.

[0015]

[Table 1]

[0016]

[Table 2]

[0017]

[Table 3]

As shown in the results of Table 1, Table 2 and Table 3, the emulsion of the present invention gave good results over all the evaluation items as compared with the emulsion of the comparative product, and in particular, improved dry feeling. It was demonstrated that the skin roughness was remarkably improved and the skin-beautifying effect was excellent. Further, the hand cream of the present invention also showed remarkable improvement as compared with the hand cream of the comparative product, and it was revealed that it has an effect of suppressing natural deterioration such as rough skin. As an opinion other than the above evaluation factors,
It was also clarified at the same time that the cosmetic of the present invention has almost no harmful effects such as stickiness during use.

[0019]

EXAMPLES Examples of the present invention will be shown below. The mixing ratio is% by weight.

Example 1 Soft Lotion (A) Purified Water 78.7 Glycerin 5.0 Propylene Glycol 4.0 Phosvitin 0.2 (B) POE (20) Sorbitan Monolaurate 1.5 POE (20) Lauryl Ether 0.5 Ethanol 10.0 Fragrance 0.1 (Preparation method) The respective components of (A) are combined and dissolved at room temperature. On the other hand, each component of (B) is also dissolved at room temperature and added to the formulation of (A) to be solubilized.

Example 2 Emulsion (A) POE (20) hydrogenated castor oil 1.5 coconut oil fatty acid monoglyceride 1.0 oleic acid triglyceride 7.5 (B) glycerin 2.5 purified water 86.8 phosvitin 0. 5 (C) Perfume 0.2 (Preparation method) Combined with each component of (A), mixed by heating, and 7
Set to 0 ° C. The components of (B) are combined and mixed by heating at 70 ° C., the formulation of (A) is added to this to emulsify, and (C) is added while cooling.

Example 3 Hand cream     (A) Vaseline 18.0           Cetanol 8.0           POE (20) oleyl ether 1.4           Sorbitan monostearate 0.8     (B) Preservative 0.3           Purified water 68.3           Phosvitin 3.0     (C) Fragrance 0.2 (Preparation method) By the same method as in Example 2.

Example 4 Hydrophilic Ointment (A) POE (30) Cetyl Ether 2.0 Glycerin Monostearate 10.0 Liquid Paraffin 10.0 Vaseline 4.0 Cetanol 5.0 Preservative 0.2 (B) Propylene glycol 10.0 Purified water 57.8 Phosvitin hydrolyzate (MW: 10,000 to 15,000) 1.0 (Preparation method) The components of (A) are combined and heated to 80 ° C. The components of (B) are combined and heated to 80 ° C.
While stirring the formulation of (A), the formulation of (B) is added thereto, and the mixture is stirred to emulsify and then cooled.

Example 5 Gel-type cosmetic oil (A) Sucrose fatty acid ester 5.0 (Daiichi Kogyo Seiyaku DK ester F-160) (B) 1,3-butylene glycol 10.0 Glycerin 24.4 Purified water 5.0 Preservative 0.3 Phosvitin 0.1 (C) Liquid paraffin 20.0 Olive oil 30.0 2-Ethylhexanoic acid triglyceride 5.0 (D) Perfume 0.2 (Preparation method) (B) Each component is combined and heated to 70 to 80 ° C., and (A) is dissolved in this. To this, the formulation of (C) is added, and when cooled to 40 ° C., (D) is added and then cooled.

Example 6 Nonalcoholic lotion (A) Propylene glycol 7.0 Polyoxyethylene (50) hydrogenated castor oil 1.0 Perfume 0.1 (B) Citric acid 0.15 Sodium citrate 0.1 Methylparaben 0.05 Purified water 91.5 Phosvitin hydrolyzate (MW: 2,000 to 5,000) 0.1 (Preparation method) Components of (A) are mixed and dissolved at room temperature or under heating, and this is dissolved. Under high-speed stirring, solubilize in addition to the formulation of (B).

[0026]

INDUSTRIAL APPLICABILITY According to the present invention, as compared with conventionally known skin roughness improving agents and moisturizers, skin conditions such as skin roughness, lack of gloss and fine wrinkles are improved, and moisture is maintained by retaining skin moisture. It can be used not only because it is remarkably excellent in skin beautifying effects such as application, but also without any harmful effect on feeling.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Yumiko Otaka             27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa             Pola Kasei Co., Ltd. Yokohama Research Center (72) Inventor Masahiro Sato             27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa             Pola Kasei Co., Ltd. Yokohama Research Center (72) Inventor Hiroyuki Nakano             27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa             Pola Kasei Co., Ltd. Yokohama Research Center

Claims (2)

[Claims] 1. A cosmetic comprising phosvitin and / or a limited hydrolyzate of phosvitin. 2. The total content of phosvitin and / or the limited hydrolyzate of phosvitin is 0.
The cosmetic according to claim 1, which is 001 to 10% by weight.