JPH05176629A - Method for culturing lentinus edodes by humus - Google Patents
Method for culturing lentinus edodes by humusInfo
- Publication number
- JPH05176629A JPH05176629A JP3360315A JP36031591A JPH05176629A JP H05176629 A JPH05176629 A JP H05176629A JP 3360315 A JP3360315 A JP 3360315A JP 36031591 A JP36031591 A JP 36031591A JP H05176629 A JPH05176629 A JP H05176629A
- Authority
- JP
- Japan
- Prior art keywords
- humus
- shiitake
- complex
- enzyme
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- Mushroom Cultivation (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、椎茸を消費者が望む品
質で、適切な時期に、必要な量だけ安定して供給するた
めの腐植による椎茸の栽培法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating shiitake mushrooms by humus for stably supplying shiitake mushrooms in a quality desired by consumers at a proper time and in a required amount.
【0002】[0002]
【従来の技術】現行の椎茸栽培法は、天候に左右される
自然栽培法の域から出られず、生産コストの大半を占め
る原木、培地の殺菌設備および種菌費用を吸収できない
不安定な栽培技術を余儀なく継続している状況にある。2. Description of the Related Art Current shiitake cultivation methods are unstable cultivation techniques that cannot be covered by natural cultivation methods that are affected by the weather and cannot absorb the cost of raw wood, medium sterilization equipment and inoculum costs that account for most of the production costs. Is forced to continue.
【0003】[0003]
【発明が解決しようとする課題】本発明は、上記の現行
技術による不安定な栽培法を解決するためになされたも
ので、その目的とするところは、良質なほだ木または栽
培培地作りの際に発生する諸問題、生産コストの大半を
占めるほだ木または栽培培地(殺菌経費を含む)の寿命
を延ばす問題、並びに施設園芸栽培化を促進することが
できる腐植による椎茸の栽培法を提供することにある。The present invention was made in order to solve the unstable cultivation method by the above-mentioned current technology, and its purpose is to make a good quality wood or cultivation medium. We provide a method for cultivating shiitake mushrooms by humus that can promote the cultivation of institutional horticultural cultivation, as well as the problems that occur in plants, the problem of extending the life of sapwood or cultivation media (including sterilization costs) that account for most of production costs. Especially.
【0004】[0004]
【課題を解決するための手段】本発明の腐植による椎茸
の栽培法は、以下に列記する通りである。The method for cultivating shiitake mushrooms by humus according to the present invention is as listed below.
【0005】1.ほだ木または栽培培地作りの際に発生
する諸問題を解決する手段。 原木または栽培培地の外面または材中の昆虫類や雑菌に
よって椎茸菌の活着や菌回り不良対策、特に培地では雑
菌で汚染された木粉等の用材の殺菌代替法としては、エ
ンザイム腐植の有機物複合体鉄錯体溶液であるFM10
0のうちの3価鉄錯体(分子量1000以上)と原木ま
たは木粉のポリフェノール類との反応により発生する過
酸化水素の殺菌力で大部分が解決される。1. Means to solve various problems that occur when making sapwood or cultivation medium. As a substitute for sterilization of Shiitake mushrooms and improper rotation of bacteria due to insects and other bacteria on the outer surface or material of raw wood or cultivation medium, especially as a sterilization alternative for materials such as wood powder contaminated with various bacteria in the medium, organic complex of enzyme humus is used. Body iron complex solution FM10
Most of them are solved by the sterilizing power of hydrogen peroxide generated by the reaction of the trivalent iron complex (molecular weight of 1000 or more) of 0 with polyphenols of raw wood or wood powder.
【0006】しかしながら、上記の反応は1回で不活性
になる。これに対して2価の鉄錯体(分子量1000以
上)のpH上昇部位における一般有機物との縮合反応の
際に発生するヒドロキシラジカルは強い殺菌力を有する
ため、本問題は全て解決する。。However, the above reaction becomes inactive once. On the other hand, the hydroxy radical generated during the condensation reaction of the divalent iron complex (molecular weight of 1000 or more) with the general organic substance at the pH-increasing site has a strong bactericidal power, and thus all the problems are solved. .
【0007】また、椎茸種菌の接種後、外部から菌回り
不良部に着生する有害菌類や椎茸菌の菌力が低下する際
に発生するほだ木黒腐れ病等の対策については、エンザ
イム腐植の有機物複合体鉄錯体(分子量1000以上)
が辺材部の壁孔壁付近のペクチン質を可溶化すること
等、椎茸菌の通路を拡張して菌回りが促進され、菌密度
が高まることによって防がれている。[0007] After the inoculation of the Shiitake inoculum, the measures against phytophytic black rot caused by harmful fungi growing on the non-rotating part from the outside and the bactericidal activity of the Shiitake bacterium are reduced with Enzyme humus. Organic complex iron complex (molecular weight 1000 or more)
Solubilizes the pectic substance in the vicinity of the wall of the sapwood, for example, and expands the passage of Shiitake fungi to promote the bacterium rotation and prevent the increase in bacterium density.
【0008】特に、栽培培地については、木粉やコーヒ
ーの抽出かすに上記FM100を加えて日光や紫外線に
曝す際、FM100中の光吸収基としてのα−カルボニ
ルにより光分解が促進され、椎茸菌が分解できる分子量
にまで低分子化されるため、活着も菌糸の伸長も加速さ
れ、有害菌による被害も防止される。In particular, regarding the cultivation medium, when the above FM100 is added to the extracted grounds of wood flour or coffee and exposed to sunlight or ultraviolet rays, photodecomposition is promoted by α-carbonyl as a light absorbing group in FM100, and Shiitake fungi are used. Since the molecular weight is reduced to such a level that it can be decomposed, survival and hyphal elongation are accelerated, and damage by harmful bacteria is prevented.
【0009】2.ほだ木または栽培培地の寿命を延ばす
問題に対する手段。 ほだ木または栽培培地の辺材部の糖類や低分子タンパク
質は勿論のこと、材の強度保持に関するリグノセルロー
ズも椎茸菌により分解接種され、年々減少する。。2. Measures to the problem of extending the life of sapwood or cultivation medium. Not only sugars and low-molecular-weight proteins in the sapwood of sapwood or cultivation medium, but also lignocelluloses, which are involved in maintaining the strength of the wood, are decomposed and inoculated by Shiitake fungi, and decrease year by year. .
【0010】特に、FM100の使用により、有機物複
合体鉄錯体の中で生理的に活性な低分子(分子量100
0以下)による椎茸菌の生育促進は2〜3倍に達するた
め、ほだ木または栽培培地の消耗は大きい。In particular, by using FM100, a physiologically active low molecule (molecular weight 100) in the organic complex iron complex is used.
Since the growth promotion of Shiitake fungi by (0 or less) reaches 2-3 times, the consumption of sapwood or cultivation medium is large.
【0011】ほだ木または栽培培地より椎茸菌に吸収さ
れ易い栄養剤として、エンザイム腐植の有機物複合体の
中で分子量1000以下の複合体をカルシウム錯体とし
て抽出し、これを栄養分運搬体として8%使用し、2%
の蔗糖と1%のリグニンスルホン酸(針葉樹)を加えた
FM200をほだ木または栽培培地中に浸透させること
により、その消耗が防がれる。As a nutrient that is easily absorbed by Shiitake fungi from sardines or cultivation medium, a complex having a molecular weight of 1000 or less is extracted as a calcium complex from an organic complex of enzyme humus, which is used as a nutrient carrier at 8%. Use, 2%
The consumption of FM200 containing 1% sucrose and 1% ligninsulfonic acid (conifer) infiltrated into a sapling or a culture medium prevents its consumption.
【0012】3.施設園芸栽培化を促進する諸問題に対
する手段。 品質、収量および生産規模の拡大については、上記FM
100および200を使用することにより解決が可能と
なるが、発生時期についても、温度刺激、含水率の調整
(水分量と酸素量刺激)以下に困難であった栄養成分の
吸収速度を制御することがFM200で可能となった
為、炭素率を変化させ、有機酸素を制御して椎茸発生時
期の調整も可能となる。3. Means for problems that promote institutional horticulture. For the expansion of quality, yield and production scale, please refer to the above FM
Although it is possible to solve the problem by using 100 and 200, it is also difficult to control the absorption rate of the nutritional component at the time of occurrence, which was difficult to do below the temperature stimulation and the adjustment of the water content (water content and oxygen content stimulation). Since it became possible with FM200, it is also possible to adjust the carbon rate and control the organic oxygen to adjust the timing of shiitake outbreak.
【0013】上記のエンザイム腐植については、中性腐
植をバイオテクノロジーによって低分子化し、これを増
量すると共に、安定化させたものであり、フルボ酸を主
体とする有機物複合体のミセルコロイドの粉体であっ
て、pHを2.8以下、酸化電位を200mV,水分を
50〜60%に調整したものである。Regarding the above-mentioned enzyme humus, neutral humus is made into a low molecular weight by biotechnology, the amount of this is increased, and it is also stabilized. A powder of micellar colloid of an organic compound complex mainly composed of fulvic acid. The pH is adjusted to 2.8 or less, the oxidation potential is adjusted to 200 mV, and the water content is adjusted to 50 to 60%.
【0014】尚、本発明で使用されるエンザイム腐植
は、落葉樹の落ち葉と動物由来の分解物が温暖な気候の
下で縮合し、地中に埋蔵されてきた中性腐植を原料と
し、バイオテクノロジーによって低分子化し、再結合さ
せ、安定化させた後、粉体としたものである。pHは
2.6以下、酸化電位は+500mV以上で、10%以
上の有機物複合体(分析上の分類におけるフルボ酸に該
当)が遊離および鉄錯体として安定な状態で含有されて
いる。有機物複合体では分子量が2000以上、100
0〜2000および1000以下の含有率は平均6:
3:1である。The enzyme humus used in the present invention is derived from a neutral humus which has been buried in the ground as a raw material by condensing the leaves of deciduous trees and decomposed products of animal origin in a warm climate. It is made into a powder after it has been reduced to a low molecular weight by, recombined, and stabilized. The pH is 2.6 or less, the oxidation potential is +500 mV or more, and 10% or more of an organic compound complex (corresponding to fulvic acid in the analytical classification) is contained in a stable state as a free and iron complex. The organic compound has a molecular weight of 2000 or more and 100
The average content of 0 to 2000 and 1000 or less is 6:
It is 3: 1.
【0015】[0015]
【実施例】以下、本発明をその実施例について説明す
る。10月下旬に伐採したコナラ原木(直径60mm、
長さ200mm)をpH3.5のFM100に2日間浸
漬し、水切り後、外樹枝が乾いた時点で種駒打ちを行
い、次いでFM200に1日浸漬した後、25℃で仮伏
せ及び本伏せを各2日および5日間行った結果、椎茸菌
の伸長は常法の3倍以上となり、常法の菌糸集落が種駒
周辺に限られていたことに比べ、仮伏せ3日で導管、仮
導管、篩管部およびその周辺部に長径一杯に広がり、本
伏せ5日では外樹の条溝部および切断面(心材部を含
む)の全面に白色菌糸の集塊が生じた。EXAMPLES The present invention will be described below with reference to examples thereof. Quercus logs (diameter 60 mm, felled in late October)
(Length 200 mm) is soaked in FM100 with pH 3.5 for 2 days, drained, seed plucked when outer branches are dry, and then soaked in FM200 for 1 day, and then temporarily laid at 25 ° C. As a result of carrying out each for 2 days and 5 days, the growth of Shiitake fungus was more than 3 times that of the conventional method, and compared to the conventional method where the mycelial colonies were limited to around the seed pieces, 3 days after the temporary disposition, the conduit and temporary conduit In the sieving tube portion and its peripheral portion, the longest diameter was spread to the fullest extent, and on the 5th day of the main felling, white mycelium agglomerates were formed on the entire groove and cut surface (including the heartwood portion) of the outer tree.
【0016】また、現行法で椎茸発生試験を行ったとこ
ろ、発生量は2倍以上の差となり、その乾燥製品も極上
の品質のものが得られ、ほだ木の寿命も2倍以上になる
ことが確認された。In addition, when a shiitake mushroom generation test was conducted by the current method, the generation amount was more than doubled, and the dried product was of the highest quality, and the life of sardine tree was more than doubled. Was confirmed.
【0017】[0017]
【発明の効果】現行の栽培法に比べて、FM100およ
びFM200を併用したものは、椎茸発量で2倍以上、
ほだ木の寿命も2倍以上延長されることが確認され、施
設園芸化が可能となる等、椎茸栽培は本発明を契機にし
て安定した経営時代を迎えることになることが期待され
る。[Effects of the Invention] Compared to the current cultivation method, the combination of FM100 and FM200 is more than twice the amount of shiitake.
It has been confirmed that the life of sardine wood is more than doubled, and it is expected that the cultivation of shiitake mushrooms will enter a stable management era with the present invention as a result of the possibility of institutional horticulture.
Claims (5)
植の主要組成である有機複合体鉄錯体溶液のFM100
(pH3.5〜4.0)に10時間以上浸漬し、水切り、
含水率の調整後、椎茸種菌を接種することにより、通常
の管理でほだ木の寿命中、有害な菌類の実害を受けるこ
となく、高品質、高収量の椎茸を生産する長寿命のほだ
木を作ることを特徴とする腐植による椎茸の栽培法。1. An organic complex iron complex solution FM100, which is a main composition of an enzyme humus, is made of a raw tree suitable for shiitake cultivation.
Immerse in (pH 3.5-4.0) for 10 hours or more, drain,
After adjusting the water content, by inoculating Shiitake inoculum with normal management, the longevity of the long-lived mushroom that produces high-quality, high-yield Shiitake without the actual damage of harmful fungi during the life of the sapwood. A method of cultivating shiitake mushrooms by humus, which is characterized by making trees.
ー叉は紅茶の抽出かす等に、エンザイム腐植の主要組成
である有機物複合体鉄錯体溶液のFM100(pH3.
5〜4.0)を10%以上加えて、日光または紫外線に
曝し、椎茸菌が分解摂取できる分子量(1000〜20
00)までの光分解を倍以上促進することを特徴とする
腐植による椎茸の栽培法。2. FM100 (pH 3.) of an organic complex iron complex solution, which is the main composition of enzyme humus, in wood flour, which is a medium material for shiitake cultivation, and extracted grounds of coffee or tea, etc.
5 to 4.0) 10% or more, and exposed to sunlight or ultraviolet rays, the molecular weight (1000 to 20) that can be decomposed and ingested by Shiitake fungi.
A method for cultivating shiitake mushrooms by humus, characterized by accelerating photodegradation up to 00) more than twice.
をEF100で処理し、椎茸種菌を接種した後、エンザ
イム腐植の有機物複合体のうち低分子複合体(分子量1
000以下)をカルシウム錯体として抽出した溶液に栄
養剤を加えたFM200(pH3.5〜4.0)に浸漬す
るか叉は撒布することにより、ほだ木または培地用材の
消耗を防ぎ、寿命を延長すると共に、接種した初年度か
ら温度、湿度、水分率、通風量が制御できる施設による
計画栽培を可能にすることを特徴とする腐植による椎茸
の栽培法。3. A low molecular weight complex (molecular weight 1) of an organic matter complex of an enzyme humus is prepared by treating a raw material for shiitake cultivation or wood flour for a medium with EF100 and inoculating a shiitake inoculum.
(000 or less) as a calcium complex is immersed in FM200 (pH 3.5 to 4.0) containing nutrients in a solution to prevent the consumption of sardines or culture medium materials and to prolong the service life. A method of cultivating shiitake mushrooms by humus, which is extended and enables planned cultivation in a facility where temperature, humidity, moisture content, and ventilation can be controlled from the first year of inoculation.
腐植粉体を30%使用した水溶液において、pHが2.
4以下、鉄錯体が500ppm以上になるまで抽出を続
け、使用の際にはpHを3.5〜4.0を目標にして30
倍希釈を行なうことを特徴とする請求項1または2に記
載の腐植による椎茸の栽培法。4. The above-mentioned FM100 has a pH of 2. in an aqueous solution containing 30% enzyme humus powder.
4 or less, continue extraction until the iron complex becomes 500 ppm or more, and when using it, aim at a pH of 3.5 to 4.0 and 30
The method for cultivating shiitake mushrooms by humus according to claim 1 or 2, wherein the doubling dilution is performed.
ム腐植粉体を30%使用した水溶液において、pHが
2.4以下になるまで抽出を続けた後、水酸化カルシウ
ムによりpHを7.0とし、高分子有機物複合体鉄錯体
(分子量1000以上)を縮合沈澱させ、残留する低分
子複合体鉄錯体を低分子複合体カルシウム錯体として抽
出し、椎茸菌の体内への栄養剤運搬体として8%使用
し、蔗糖2%ペプトン1%、リグニンスルホン酸1%を
加えた水溶液とし、硫酸でpHを3.5〜4.0に調整し
て用いることを特徴とする請求項3に記載の腐植による
椎茸の栽培法。5. In the above FM200, extraction is continued in an aqueous solution containing 30% enzyme humus powder until the pH becomes 2.4 or less, and then the pH is adjusted to 7.0 with calcium hydroxide. , High-molecular organic complex iron complex (molecular weight 1000 or more) is condensed and precipitated, and the remaining low-molecular complex iron complex is extracted as a low-molecular complex calcium complex, 8% as a nutrient transporter into the body of Shiitake bacterium. The humus according to claim 3, which is used as an aqueous solution containing 2% sucrose and 1% peptone and 1% ligninsulfonic acid and adjusted to pH 3.5 to 4.0 with sulfuric acid. Shiitake cultivation method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3360315A JPH05176629A (en) | 1991-12-27 | 1991-12-27 | Method for culturing lentinus edodes by humus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3360315A JPH05176629A (en) | 1991-12-27 | 1991-12-27 | Method for culturing lentinus edodes by humus |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH05176629A true JPH05176629A (en) | 1993-07-20 |
Family
ID=18468873
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3360315A Pending JPH05176629A (en) | 1991-12-27 | 1991-12-27 | Method for culturing lentinus edodes by humus |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH05176629A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019097528A (en) * | 2017-12-07 | 2019-06-24 | 株式会社日本ソフケン | Mushroom bed cultivation method using fulvic acid |
JP2021029219A (en) * | 2019-08-29 | 2021-03-01 | 株式会社ハイファ研究所 | Method for cultivating shiitake mushroom using coffee residue, and method for increasing functional components |
JP2023072715A (en) * | 2021-11-14 | 2023-05-25 | 株式会社日本ソフケン | Method of producing humic substance solution and humic substance |
-
1991
- 1991-12-27 JP JP3360315A patent/JPH05176629A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019097528A (en) * | 2017-12-07 | 2019-06-24 | 株式会社日本ソフケン | Mushroom bed cultivation method using fulvic acid |
JP2021029219A (en) * | 2019-08-29 | 2021-03-01 | 株式会社ハイファ研究所 | Method for cultivating shiitake mushroom using coffee residue, and method for increasing functional components |
JP2023072715A (en) * | 2021-11-14 | 2023-05-25 | 株式会社日本ソフケン | Method of producing humic substance solution and humic substance |
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