JPH051712B2 - - Google Patents
Info
- Publication number
- JPH051712B2 JPH051712B2 JP62204103A JP20410387A JPH051712B2 JP H051712 B2 JPH051712 B2 JP H051712B2 JP 62204103 A JP62204103 A JP 62204103A JP 20410387 A JP20410387 A JP 20410387A JP H051712 B2 JPH051712 B2 JP H051712B2
- Authority
- JP
- Japan
- Prior art keywords
- benzyl ester
- glutamic acid
- copolymer
- alanine
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229960002989 glutamic acid Drugs 0.000 claims description 49
- 239000004220 glutamic acid Substances 0.000 claims description 47
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- 235000013922 glutamic acid Nutrition 0.000 claims description 46
- 229960003767 alanine Drugs 0.000 claims description 41
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 40
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 39
- 239000000463 material Substances 0.000 claims description 35
- 229920001577 copolymer Polymers 0.000 claims description 34
- 238000004113 cell culture Methods 0.000 claims description 33
- 235000004279 alanine Nutrition 0.000 claims description 32
- 229920005604 random copolymer Polymers 0.000 claims description 26
- 238000009408 flooring Methods 0.000 claims description 16
- BGGHCRNCRWQABU-JTQLQIEISA-N (2s)-2-amino-5-oxo-5-phenylmethoxypentanoic acid Chemical group OC(=O)[C@@H](N)CCC(=O)OCC1=CC=CC=C1 BGGHCRNCRWQABU-JTQLQIEISA-N 0.000 claims description 14
- 229940024606 amino acid Drugs 0.000 claims description 14
- 235000001014 amino acid Nutrition 0.000 claims description 14
- 150000002308 glutamine derivatives Chemical group 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- JXTHNDFMNIQAHM-UHFFFAOYSA-N dichloroacetic acid Chemical compound OC(=O)C(Cl)Cl JXTHNDFMNIQAHM-UHFFFAOYSA-N 0.000 claims description 12
- -1 hydroxyethylaminoethyl group Chemical group 0.000 claims description 12
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- 238000012258 culturing Methods 0.000 claims description 9
- 125000004185 ester group Chemical group 0.000 claims description 8
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- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims description 5
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- 125000003412 L-alanyl group Chemical group [H]N([H])[C@@](C([H])([H])[H])(C(=O)[*])[H] 0.000 claims 2
- 125000003338 L-glutaminyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C([H])([H])C(=O)N([H])[H] 0.000 claims 2
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- QPFMBZIOSGYJDE-UHFFFAOYSA-N 1,1,2,2-tetrachloroethane Chemical compound ClC(Cl)C(Cl)Cl QPFMBZIOSGYJDE-UHFFFAOYSA-N 0.000 description 10
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- 239000011677 pyridoxine Substances 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 229960001153 serine Drugs 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 206010046766 uterine cancer Diseases 0.000 description 1
- 229960004295 valine Drugs 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polyamides (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62204103A JPS6447373A (en) | 1987-08-19 | 1987-08-19 | Bed material for cell culture and cell culture using said bed material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62204103A JPS6447373A (en) | 1987-08-19 | 1987-08-19 | Bed material for cell culture and cell culture using said bed material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6447373A JPS6447373A (en) | 1989-02-21 |
| JPH051712B2 true JPH051712B2 (https=) | 1993-01-08 |
Family
ID=16484848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62204103A Granted JPS6447373A (en) | 1987-08-19 | 1987-08-19 | Bed material for cell culture and cell culture using said bed material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6447373A (https=) |
-
1987
- 1987-08-19 JP JP62204103A patent/JPS6447373A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6447373A (en) | 1989-02-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |