JPH0452733B2 - - Google Patents
Info
- Publication number
- JPH0452733B2 JPH0452733B2 JP1018344A JP1834489A JPH0452733B2 JP H0452733 B2 JPH0452733 B2 JP H0452733B2 JP 1018344 A JP1018344 A JP 1018344A JP 1834489 A JP1834489 A JP 1834489A JP H0452733 B2 JPH0452733 B2 JP H0452733B2
- Authority
- JP
- Japan
- Prior art keywords
- inflorescences
- dwarf
- water
- culturing
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000196324 Embryophyta Species 0.000 claims description 26
- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical compound CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 6
- 239000000049 pigment Substances 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 239000004552 water soluble powder Substances 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims 1
- 239000006870 ms-medium Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000218922 Magnoliophyta Species 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 241000723353 Chrysanthemum Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 206010013883 Dwarfism Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 240000002395 Euphorbia pulcherrima Species 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- 241000985694 Polypodiopsida Species 0.000 description 2
- 241000960400 Torenia Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- 235000010746 mayonnaise Nutrition 0.000 description 2
- 239000008268 mayonnaise Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 235000015701 Artemisia arbuscula Nutrition 0.000 description 1
- 235000002657 Artemisia tridentata Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 241000555825 Clupeidae Species 0.000 description 1
- 241000428813 Gomphrena Species 0.000 description 1
- 240000001549 Ipomoea eriocarpa Species 0.000 description 1
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 description 1
- 240000007015 Melilotus indicus Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000208422 Rhododendron Species 0.000 description 1
- 241001247145 Sebastes goodei Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000019512 sardine Nutrition 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本願発明は、矮化せんにちこうの栽培方法に関
するものである。更に詳細に述べれば、密閉容器
中で矮化せんにちこうを栽培して開花させ、その
まま長時間にわたる観賞が可能な矮化せんにちこ
うの栽培方法に関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for cultivating dwarf chiliflower. More specifically, the present invention relates to a method for cultivating dwarf dwarf dwarfs in which the dwarf dwarf dwarfs are cultivated in a closed container, allowed to bloom, and can be admired as is for a long period of time.
密閉容器中で矮化せんにちこうを栽培して開花
させ、そのまま観賞可能とする矮化せんにちこう
の栽培方法は未開発である。
A method for cultivating dwarf dwarf chiliflower in which the dwarf dwarf chiliflower is cultivated in an airtight container and allowed to bloom so that it can be admired as is has not yet been developed.
そもそも、密閉容器中で草花を栽培し、開花さ
せることは大変困難であり、矮化トレニアについ
て成功した報告（特開昭60−221020号公報）があ
るのみである。この矮化トレニアも、今日迄商品
化されるに至つていない。現在、密閉容器中で矮
化植物を育成し、観賞用に供されているものは、
花をつけない観葉植物ばかりである。 In the first place, it is very difficult to cultivate and flower flowers in a closed container, and there is only one successful report on dwarf Torenia (Japanese Patent Laid-Open No. 60-221020). This dwarf Torenia has also not been commercialized to date. Currently, dwarf plants are grown in airtight containers and used for ornamental purposes.
They are all ornamental plants that do not produce flowers.
本発明者も、多くの種類の草花について、密閉
容器中で矮化させ、しかも開花させる条件を矮化
剤の種類、矮化剤の濃度、照明等の栽培条件を中
心に研究してきたが、容易には開花に成功しなか
つた。しかしながら、今般、せんにちこうに関し
て、密閉容器中で矮化させ、しかも開花させるた
めの新知見を得、ここに本発明を完成した。
The present inventor has also studied the conditions for dwarfing and flowering many types of flowers in airtight containers, focusing on the type of dwarfing agent, the concentration of the dwarfing agent, and cultivation conditions such as lighting. It did not easily succeed in flowering. However, we have recently obtained new knowledge for dwarfing and blooming sagebrush in a closed container, and have now completed the present invention.
即ち、本発明は次の２発明である。 That is, the present invention consists of the following two inventions.
第１番目の発明は、透視可能な容器中に入れた
農林水産省登録第12842号に係る矮化剤（成分：
ダミノジツト〔Ｎ−（ジメチルアミノ）−スクシン
アミド酸〕80.8％、色素及び水溶性無機塩等20.0
％、性状：淡桃色水溶性粉末）２〜10ｇ／添加
済のムラシゲスクーグ培地にせんにちこうの無菌
幼苗体の全草を植えつけ、しかる後2000〜3000ル
ツクスで６〜10時間／１日の照明条件下で無菌的
に20〜70日間にわたつて密閉培養することを特徴
とする矮化せんにちこうの栽培方法である。 The first invention is a dwarfing agent (ingredients:
Daminozite [N-(dimethylamino)-succinamic acid] 80.8%, pigments and water-soluble inorganic salts, etc. 20.0
%, properties: light pink water-soluble powder) 2 to 10 g/added Murashigeskoog medium is planted with whole plants of sterile seedlings of Sennichiko, and then grown at 2000 to 3000 lux for 6 to 10 hours/1 day. This is a method for cultivating dwarf dwarf chiliflower, which is characterized by culturing the dwarf dwarf in an aseptically sealed manner for 20 to 70 days under illumination conditions.
第２番目の発明は、透視可能な容器中に入れた
農林水産省登録第12842号に係る矮化剤（成分：
ダミノジツト〔Ｎ−（ジメチルアミノ）−スクシン
アミド酸〕80.8％、色素及び水溶性無機塩等20.0
％、性状：淡桃色水溶性粉末）２〜10ｇ／添加
済のムラシゲスクーグ培地にせんにちこうの無菌
幼苗体の全草を植えつけ、しかる後2000〜3000ル
ツクスで６〜10時間／１日の照明条件下で無菌的
に20〜70日間にわたつて密閉培養して花序を形成
したせんにちこうを得、次いで前記花序を切断
し、前記と同種の矮化剤１〜10ｇ／添加のムラ
シゲスクーグ培地に前記切断花序を植けつけ、し
かる後2000〜3000ルツクスで６〜10時間／１日の
照明条件下で無菌的に７〜10日間にわたつて密閉
培養することを特徴とする矮化せんにちこうの栽
培方法である。 The second invention is a dwarfing agent (ingredients:
Daminozite [N-(dimethylamino)-succinamic acid] 80.8%, pigments and water-soluble inorganic salts, etc. 20.0
%, properties: light pink water-soluble powder) 2 to 10 g/added Murashigeskoog medium is planted with whole plants of sterile seedlings of Sennichiko, and then grown at 2000 to 3000 lux for 6 to 10 hours/1 day. The inflorescences were obtained by culturing in closed conditions under light conditions for 20 to 70 days in an aseptic manner, and then the inflorescences were cut, and 1 to 10 g of a dwarfing agent of the same species as above was added. A dwarfing plant characterized in that the cut inflorescences are planted in a medium and then cultured in a sealed manner aseptically under lighting conditions of 2000 to 3000 lux for 6 to 10 hours/day for 7 to 10 days. This is a method of cultivating Japanese cabbage.
本発明における「せんにちこう」は、ヒユ科
Gomphrena属中の代表的種である。そしてせん
にちこうは、「茎は直立して高さ80cmに達し、節
の部分が太まり、全草にあらい毛がある。葉は対
生し、長楕円形で、長さ４〜10cm、柄のもとのほ
うが節とともに赤みをおびる。７〜10月ころ、茎
の上部が枝分かれして花をつける。」（平凡社発
行：世界大百科事典）ものである。 "Senichikou" in the present invention means Amaranthaceae
It is a representative species of the Gomphrena genus. ``The stem is erect and reaches a height of 80 cm, the nodes are thick, and the whole plant is hairy.The leaves are opposite, oblong, and 4 to 10 cm long. The base of the stem turns reddish along with the nodes.The upper part of the stem branches and produces flowers from July to October.'' (published by Heibonsha: World Encyclopedia).
本発明では、農林水産省登録第12842号に係る
矮化剤（成分：ダミノジツト〔Ｎ−（ジメチルア
ミノ）−スクシンアミド酸〕80.8％、色素及び水
溶性無機塩等20.0％、性状：淡桃色水溶性粉末）
を使用する。この矮化剤は、「ビーナイン」（日本
曹達株式会社の登録商標−以下同じ）の名称で市
販（製造及び販売：日本曹達株式会社）されてい
る。 In the present invention, the dwarfing agent according to Ministry of Agriculture, Forestry and Fisheries Registration No. 12842 (ingredients: Daminosit [N-(dimethylamino)-succinamic acid] 80.8%, pigments and water-soluble inorganic salts, etc. 20.0%, property: pale pink water-soluble) powder)
use. This dwarfing agent is commercially available (manufactured and sold by Nippon Soda Co., Ltd.) under the name "B-Nine" (registered trademark of Nippon Soda Co., Ltd. - the same applies hereinafter).
ビーナインは、節間の伸長を抑制して植物の草
丈を低くする新しい矮化剤で、キク、ポインセチ
ア、ハイドランジア、アサガオ等の草花及びアザ
レア等の花木用矮化剤である。ビーナインの説明
書には、用いる植物の種類や品種によつて微妙な
効果の差があると記載されているが、密閉容器中
で上記のキク、ポインセチア、ハイドランジアを
矮化させ且つ開花させることは本発明者の幅広い
条件設定による実験でも不可であり、唯一「せん
にちこう」に顕著な効果が認められた。 B-Nine is a new dwarfing agent that reduces the height of plants by suppressing the elongation of internodes, and is a dwarfing agent for flowering plants such as chrysanthemum, poinsettia, hydrangia, and morning glory, as well as flowering trees such as azalea. The B-Nine instruction manual states that there are subtle differences in effectiveness depending on the type and variety of plants used, but it is not possible to dwarf and bloom the chrysanthemums, poinsettias, and hydrangia mentioned above in an airtight container. Even in experiments conducted by the present inventor under a wide range of conditions, this was not possible, and only "Senichiko" was found to have a remarkable effect.
せんにちこうの全草を密閉培養するに当たつて
は、ビーナインをムラシゲスクーグ培地（以下、
「MS培地」という）中に２〜10ｇ／添加して
用いる。ビーナインの添加量が、２ｇ／未満で
は矮化効果が不十分であり、10ｇ／を越えると
薬害により、せんにちこうが枯死する率が高くな
る。 When culturing the whole plant of Sennichiko in a closed culture, B-Nine is grown in Murashigeskoog medium (hereinafter referred to as
It is used by adding 2 to 10 g/into (referred to as "MS medium"). If the amount of B-Nine added is less than 2 g/l, the dwarfing effect will be insufficient, and if it exceeds 10 g/l, the rate of withering and death of the ferns will increase due to chemical damage.
一方、せんにちこうの花序部分のみを切断し、
この花序部分を密閉培養するに当たっては、ビー
ナインをMS培地中に１〜10ｇ／添加して用い
る。ビーナインの添加量が、１ｇ／未満では矮
化効果が不十分であり、10ｇ／を越えると薬害
により、せんにちこうが枯死する率が高くなる。 On the other hand, cut only the inflorescence part of Sennichiko,
When culturing this inflorescence part in a sealed manner, 1 to 10 g of B-Nine is added to the MS medium. If the amount of B-Nine added is less than 1 g/, the dwarfing effect will be insufficient, and if it exceeds 10 g/, the rate of withering and death of the ferns will increase due to chemical damage.
なお、30℃以上の高温条件下（夏季）でせんに
ちこうを培養する場合には、ビーナインの添加量
が５ｇ／以下では生長が早く、観賞期間が短く
なるので、５ｇ／以上とすべきである。 In addition, when culturing senichiko under high temperature conditions of 30℃ or higher (summer), the amount of B-Nine added should be 5g/or more, as if it is less than 5g, the growth will be rapid and the ornamental period will be shortened. It is.
培養に当たつては光を照射するが、その照度
は、約2000〜3000ルツクスの範囲が好適である。
2000ルツクス未満では生長が十分に行われないの
で培養基間がいたづらに長くなり、3000ルツクス
を超える照明は過剰である。また、照明時間は、
１日（24時間）のうち、約６〜10時間とするのが
好適である。６時間／１日未満では生長が十分に
行われないので培養基間がいたづらに長くなり10
時間／１日を超えると、生長が促進される結果、
せんにちこうが徒長して鑑賞に耐えられなくな
る。 During cultivation, light is irradiated, and the illumination intensity is preferably in the range of about 2000 to 3000 lux.
If the illumination is less than 2,000 lux, the growth will not be sufficient and the distance between the culture media will be long, and if the illumination is more than 3,000 lux, it will be excessive. In addition, the lighting time is
Approximately 6 to 10 hours per day (24 hours) is suitable. If it is less than 6 hours/1 day, the growth will not be sufficient and the gap between the culture media will become longer10
If the time exceeds 1 day, growth will be promoted, resulting in
The children's children become so arrogant that they can't bear to watch it anymore.
照明期間（培養期間）は、せんにちこうの全草
を密閉培養するに当たつては約20〜70日間の範
囲、せんにちこうの花序部分のみを切断し、この
花序部分を密閉培養するに当たつては約７〜10日
間の範囲が好適である。 The lighting period (cultivation period) is approximately 20 to 70 days when culturing the whole plant of Sennichiko in a sealed manner. In this case, a period of about 7 to 10 days is suitable.
前記各照明期間よりも短いと、植物体が十分に
生長せず、通常の室内に放置するときに枯死する
確立が高くなる。一方、前記照明期間よりも長い
と、いたずらに培養期間を長びかせるだけで不経
済であるとともに、培養中に花序が開花してしま
うので、出荷後の鑑賞期間が短くなる。 If the lighting period is shorter than each of the above-mentioned lighting periods, there is a high probability that the plant will not grow sufficiently and will wither when left indoors. On the other hand, if it is longer than the above-mentioned illumination period, the cultivation period will be unnecessarily prolonged, which is uneconomical, and the inflorescences will bloom during cultivation, resulting in a shortened viewing period after shipment.
本発明に係る矮化せんにちこうの栽培方法は、
透視可能な密閉容器中で行われる。透視可能な容
器としては、三角フラスコ、試験管、瓶等を挙げ
ることができ、これらから任意に選択使用すれば
よい。 The method for cultivating dwarf chiliflower according to the present invention includes:
It is carried out in a transparent container. Examples of containers that can be seen through include Erlenmeyer flasks, test tubes, and bottles, and any one of these may be used.
また、本発明に係る矮化せんにちこうの栽培方
法は、無菌的培養でなければならない。さもなけ
れば、植物体はカビ、ウイルス、細菌に侵されて
枯死する。 In addition, the method for cultivating dwarf chili peppers according to the present invention must be carried out in an aseptic manner. Otherwise, the plant will be attacked by mold, viruses, and bacteria and die.
以下に本発明の代表的な実施例を示し、更に本
発明の詳細を説明する。
Below, typical examples of the present invention will be shown and further details of the present invention will be explained.
実施例 １
（せんにちこうの全草を利用する場合）
せんにちこうの種子２ｇを200ml三角フラスコ
に入れ、ツイーン（Tween）20を0.1％を添加し
た滅菌水を注ぎ、アンチホルミンを10ml加えて撹
拌後放置した。10分後に種子を滅菌水で２回洗浄
し、水を切つた後70％エタノールを200ml入れて
放置した。10分後に添加物を含まない滅菌水で２
回洗浄し、無菌種子とした。Example 1 (When using the whole plant of Senni Chikou) Put 2 g of Senni Chikou seeds into a 200 ml Erlenmeyer flask, pour in sterile water containing 0.1% Tween 20, and add 10 ml of antiformin. After addition, the mixture was stirred and left to stand. After 10 minutes, the seeds were washed twice with sterilized water, drained, and left in 200 ml of 70% ethanol. After 10 minutes, rinse with sterile water without additives.
The seeds were washed twice and made into sterile seeds.
上記の無菌種子を、寒天0.9％のMS培地を入れ
た試験管に５粒ずつ置床し、25℃暗条件で放置し
た。１週間後に汚染率及び発芽率を調査した結
果、汚染率は試験管の本数レベルで70％、発芽率
は個体レベルで55％であつた。汚染されていない
発芽分の試験管の植物体を25℃の室内において、
照度：3000ルツクス、照明時間：10時間／１日、
照明期間：２週間の照明条件下で培養し、無菌幼
植物体を得た。 Five of the above sterile seeds were placed in a test tube containing MS medium containing 0.9% agar and left in the dark at 25°C. As a result of investigating the contamination rate and germination rate one week later, the contamination rate was 70% at the number of test tubes level, and the germination rate was 55% at the individual level. Place the uncontaminated germinated plants in test tubes in a room at 25°C.
Illuminance: 3000 lux, lighting time: 10 hours/day,
Lighting period: Cultured under light conditions for 2 weeks to obtain sterile seedlings.
このようにして得た無菌幼植物体の中から茎直
径が1.0mmのもの30本を選び出し、200mlの規格瓶
（通称マヨネーズ瓶）10個に入れられたビーナイ
ン５ｇ／添加済のMS培地に前記無菌幼植物体
の全草３本ずつを植え付け、耐熱性の透明プラス
チツク製の蓋をして、25℃の室内において、3000
ルツクス、照明時間：６時間／１日、照明期間：
70日間の照明条件下で培養した。植え付け後20日
間で30％の個体に花序が形成され、70日間で鑑賞
に耐える花序が形成された。植え付け時に、平均
して茎直径1.0mm、茎高さ３cm、花序直径0.3cm、
花序長さ0.3cmであつたものが、70日経過後では、
平均して、茎直径は2.0mm、茎高さ８cm、花序直
径1.2cm、花序長さ1.2cmであつた。 From the sterile seedlings obtained in this way, 30 plants with a stem diameter of 1.0 mm were selected and placed in MS medium containing 5 g of Be-Nine in 10 200 ml standard bottles (commonly known as mayonnaise bottles). Plant 3 whole plants of sterile seedlings, cover with heat-resistant transparent plastic lids, and incubate in a room at 25℃ for 3,000 hours.
Lux, lighting time: 6 hours/1 day, lighting period:
Cultured under light conditions for 70 days. Inflorescences were formed in 30% of the plants 20 days after planting, and inflorescences that were suitable for viewing were formed within 70 days. At the time of planting, the average stem diameter is 1.0 mm, stem height is 3 cm, and inflorescence diameter is 0.3 cm.
The inflorescence was 0.3 cm long, but after 70 days,
On average, the stem diameter was 2.0 mm, the stem height was 8 cm, the inflorescence diameter was 1.2 cm, and the inflorescence length was 1.2 cm.
規格瓶10個のうち５個については引き続き同一
照明条件下で培養し、残りの５個については直射
日光の当らない窓際に放置して培養した結果、共
に５ケ月以上に亘つて花序を形成し続け、開花し
た植物体を生体のまま維持させることができた。
なお、この間水分及び栄養分の供給は１回も行な
わなかつた。 Five of the 10 standard bottles were continued to be cultured under the same lighting conditions, and the remaining five were left to grow by a window out of direct sunlight. As a result, both of them formed inflorescences for over 5 months. As a result, they were able to maintain the flowering plant as a living organism.
Note that during this period, water and nutrients were not supplied even once.
なお、別の実験において、MS培地に0.1〜1.0
％のカイネチン（ホルモン）を添加しておくと、
花序の形成数が多くなり、さらに鑑賞価値が高く
なることを確認した。 In addition, in another experiment, 0.1 to 1.0
If you add % of kinetin (hormone),
It was confirmed that the number of inflorescences formed increases and the ornamental value becomes even higher.
実施例 ２
（せんにちこうの花序部分を利用する場合）
実施例１の同様にして作出したせんにちこうの
無菌幼植物体の中から、茎直径約1.0mmのものを
30本選び出し、450mlの規格瓶（通称マヨネーズ
瓶）10個に入れられたビーナイン５ｇ／を添加
したMS培地に前記無菌幼植物体の全草３本ずつ
を植え付け、耐熱性の透明プラスチツク製の蓋を
して、25℃の室内において、照度：3000ルツク
ス、照明時間：６時間／１日、照明期間：70日間
の照明条件下で培養して、花序が形成されたせん
にちこうを得た。Example 2 (When using the inflorescence part of Sennichikou) From among the sterile young plants of Sennichikou produced in the same manner as in Example 1, those with a stem diameter of approximately 1.0 mm were used.
Thirty plants were selected, and three whole plants of the above-mentioned sterile seedlings were planted in MS medium supplemented with 5 g of B-Nine in ten 450 ml standard bottles (commonly known as mayonnaise bottles), and the plants were placed in heat-resistant transparent plastic lids. The plants were then cultured in a room at 25°C under the following lighting conditions: illuminance: 3000 lux, lighting time: 6 hours/day, lighting period: 70 days, to obtain flowers with inflorescences formed. .
次いで、このせんにちこうの花序部分のみ（花
序に隣接する葉２枚を含むこともある）を切断
（茎の長さは約１cm）した。この花序部分３個づ
つを、200mlの規格瓶10個に入れられたビーナイ
ン５ｇ／添加済のMS培地に植え付け、耐熱性
のプラスチツク製の蓋をして、25℃の室内におい
て、照度3000ルツクス、照明時間：６時間／１
日、照明期間：10日間の照明条件下で培養を続け
た。10日間で花序は培地に挿入している茎の切断
部分から発根し、地上部分は次々と花序を形成し
続け、葉がない状態、または花序に隣接する葉２
枚のみをつけた状態で生長を続け、70日間では平
均して花序直径1.2cm、花序高さ1.2cmとなつた。 Next, only the inflorescence part (sometimes including 2 leaves adjacent to the inflorescence) of this senji was cut (the length of the stem was approximately 1 cm). Three of these inflorescences were planted in MS medium supplemented with 5 g of Be-Nine in 10 200 ml standard bottles, covered with a heat-resistant plastic lid, and placed in a room at 25°C under an illuminance of 3000 lux. Lighting time: 6 hours/1
Days, lighting period: Culture was continued under lighting conditions for 10 days. In 10 days, the inflorescence will root from the cut part of the stem inserted into the medium, and the above-ground part will continue to form inflorescences one after another, with either no leaves or two leaves adjacent to the inflorescence.
It continued to grow with only leaves attached, and in 70 days, the inflorescence diameter was 1.2 cm and the inflorescence height was 1.2 cm on average.
規格瓶10個のうち５個については引き続き同一
照明条件下で培養し、残りの５個については直射
日光の当らない窓際に放置して培養した結果、共
に４ヶ月以上に亘つて花序を形成し続け、開花し
た植物体を生体のまま維持した。 Five of the 10 standard bottles were continued to be cultured under the same lighting conditions, and the remaining five were left to grow by a window out of direct sunlight. As a result, both of them formed inflorescences for over 4 months. The flowering plants were then maintained as living organisms.
なお、別の実験において、MS培地に0.1〜1.0
％のカイネチン（ホルモン）を添加しておくと、
花序の分岐及び側芽の形成数が多くなり、さらに
鑑賞価値が高くなることを確認した。 In addition, in another experiment, 0.1 to 1.0
If you add % of kinetin (hormone),
It was confirmed that the number of branching of the inflorescence and the formation of lateral buds increased, further increasing the ornamental value.
更に補足説明をすると、アメリカせんにちこう
も上記実施例１、２と同一条件で栽培できる。 As a supplementary explanation, American sardines can also be cultivated under the same conditions as in Examples 1 and 2 above.
本発明は、密閉容器中に特定の矮化剤を入れ、
照度：2000〜3000ルツクス、照明時間：６〜10時
間／１日、照明期間：せんにちこうの全草を密閉
培養する場合には20〜70日間、せんにちこうの花
序部分のみを切断し、この花序部分を密閉培養す
る場合には７〜10日間という特定の培養条件下で
せんにちこうを栽培するので、せんにちこうを徒
長させることなく開花させ、そのままの状態を長
期間にわたつて保持することができる。
The present invention involves placing a specific dwarfing agent in a sealed container,
Illuminance: 2000 to 3000 lux, lighting time: 6 to 10 hours/day, lighting period: If the whole plant of Sennichiko is cultivated in a closed culture, cut only the inflorescence part of Sennichiko for 20 to 70 days. However, when culturing this inflorescence part in a sealed manner, the sennichiko is cultivated under specific culture conditions for 7 to 10 days, so the sennichiko is allowed to bloom without elongating and can remain in that state for a long period of time. can be held for a period of time.
本発明は以上説明した通りの矮化せんにちこう
の栽培方法である。この栽培方法によつて初めて
得られた密閉容器中の開花した矮化せんにちこう
は、４ケ月以上に亘つて開花状態を維持し続け、
その間、水分及び栄養分の供給は全く必要なく、
容器が小さいことからテーブル、棚等容器を置く
スペースさえあればいかなる場合にも飾ることが
でき、矮化せんにちこうが開花しているというめ
ずらしさが加わり、商品化の十分可能な産業利用
価値の高いものである。
The present invention is a method for cultivating dwarf chiliflower as explained above. The flowering dwarf niachikou in an airtight container, which was obtained for the first time using this cultivation method, maintained its flowering state for more than four months.
During this time, there is no need for any supply of water or nutrients.
Because the container is small, it can be displayed on any occasion as long as there is space to place the container, such as on a table or shelf, and with the added rarity of a dwarf dwarf flowering plant, it is suitable for industrial use and can be commercialized. It is of high value.
Claims (1)
12842号に係る矮化剤（成分：ダミノジツト〔Ｎ
−（ジメチルアミノ）−スクシンアミド酸〕80.8
％、色素及び水溶性無機塩等20.0％、性状：淡桃
色水溶性粉末）２〜10ｇ／添加済のムラシゲス
クーグ培地にせんにちこうの無菌幼苗体の全草を
植えつけ、しかる後2000〜3000ルツクスで６〜10
時間／１日の照明条件下で無菌的に20〜70日間に
わたつて密閉培養することを特徴とする矮化せん
にちこうの栽培方法。 ２ 透視可能な容器中に入れた農林水産省登録第
12842号に係る矮化剤（成分：ダミノジツト〔Ｎ
−（ジメチルアミノ）−スクシンアミド酸〕80.8
％、色素及び水溶性無機塩等20.0％、性状：淡桃
色水溶性粉末）２〜10ｇ／添加済のムラシゲス
クーグ培地にせんにちこうの無菌幼苗体の全草を
植えつけ、しかる後2000〜3000ルツクスで６〜10
時間／１日の照明条件下で無菌的に20〜70日間に
わたつて密閉培養して花序を形成したせんにちこ
うを得、次いで前記花序を切断し、前記と同種と
矮化剤１〜10ｇ／添加のムラシゲスクーグ培地
に前記切断花序を植けつけ、しかる後2000〜3000
ルツクスで６〜10時間／１日の照明条件下で無菌
的に７〜10日間にわたつて密閉培養することを特
徴とする矮化せんにちこうの栽培方法。[Scope of Claims] 1. The Ministry of Agriculture, Forestry and Fisheries registered No.
Dwarfing agent according to No. 12842 (component: Daminojitsu [N
-(dimethylamino)-succinamic acid〕80.8
%, pigments and water-soluble inorganic salts, etc. 20.0%, properties: light pink water-soluble powder) 2-10g/added whole plant of sterile seedlings of Sennichiko is planted in Murashigeskoog medium, and then 2000-3000 6-10 in Lutx
A method for cultivating dwarf dwarf Chinese cabbage, which is characterized by culturing sterilely under closed lighting conditions for 20 to 70 days. 2 Ministry of Agriculture, Forestry and Fisheries registration number placed in a transparent container
Dwarfing agent according to No. 12842 (component: Daminojitsu [N
-(dimethylamino)-succinamic acid〕80.8
%, pigments and water-soluble inorganic salts, etc. 20.0%, properties: light pink water-soluble powder) 2-10g/added whole plant of sterile seedlings of Sennichiko is planted in Murashigeskoog medium, and then 2000-3000 6-10 in Lutx
The inflorescences are obtained by culturing them in a hermetic manner for 20 to 70 days under lighting conditions of time/day to obtain the inflorescences, and then cutting the inflorescences and culturing them with the same species as above and dwarfing agents 1 to 1. The cut inflorescences were planted in Murashigeskoog medium containing 10g/addition, and then 2000 to 3000
A method for cultivating dwarf chiliflower in an aseptically sealed manner for 7 to 10 days under illumination conditions of 6 to 10 hours/day using Lutucus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1018344A JPH02200121A (en) | 1989-01-26 | 1989-01-26 | Culture of dwarf glove amaranth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1018344A JPH02200121A (en) | 1989-01-26 | 1989-01-26 | Culture of dwarf glove amaranth |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02200121A JPH02200121A (en) | 1990-08-08 |
| JPH0452733B2 true JPH0452733B2 (en) | 1992-08-24 |
Family
ID=11969040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1018344A Granted JPH02200121A (en) | 1989-01-26 | 1989-01-26 | Culture of dwarf glove amaranth |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02200121A (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4886213B2 (en) * | 2005-05-18 | 2012-02-29 | 福花園種苗株式会社 | Eustoma plant cultivation method |
| JP5853363B2 (en) * | 2009-12-11 | 2016-02-09 | 住友化学株式会社 | How to reduce the effects of temperature stress on plants |
| JP5602499B2 (en) * | 2010-05-31 | 2014-10-08 | 日本曹達株式会社 | Liquid composition |
| CN103718955B (en) * | 2013-11-20 | 2016-02-03 | 青岛佰众化工技术有限公司 | A kind of abductive approach of globe amaranth regeneration plant |
| JP6373117B2 (en) * | 2014-08-07 | 2018-08-15 | タキイ種苗株式会社 | Brush caoleracea seed plant and method for producing the same |
| CN107683766A (en) * | 2016-08-05 | 2018-02-13 | 通化东宝药业股份有限公司 | Ginseng tissue culture adventitious root biological reaction apparatus |
| CN107484531A (en) * | 2017-09-26 | 2017-12-19 | 界首市朝鹏家庭农场 | A kind of high-yield planting method of three-coloured amaranth |
| CN109380079A (en) * | 2018-10-29 | 2019-02-26 | 张志安 | One kind spending more globe amaranth implantation methods |
-
1989
- 1989-01-26 JP JP1018344A patent/JPH02200121A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02200121A (en) | 1990-08-08 |
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