JPH045004Y2 - - Google Patents

Description

[Detailed description of the invention] (a) Industrial application field This invention relates to a cooling device for electrophoresis, and more specifically to a cooling device that efficiently removes Joule heat generated during electrophoresis.

(B) Prior art Generally, an electrophoresis apparatus is equipped with a cooling section in order to control the temperature of the electrophoresis gel, that is, to keep the temperature of the electrophoresis gel constant and to make the temperature distribution uniform over the entire surface of the gel. is provided.

This cooling section requires good thermal conductivity in order to efficiently remove the Joule heat generated during electrophoresis, but it also needs to have high insulation properties required for electrophoresis, so conventionally It was made of glass, acrylic, etc.

(c) Problems to be solved by the invention However, glass, acrylic, and the like have low thermal conductivity, so the Joule heat cannot be efficiently removed. Therefore, there was a drawback that the separation ability and the reproducibility of electrophoresis data were reduced.

This invention was devised in view of the above circumstances, and its purpose is to efficiently remove Joule heat generated during electrophoresis to improve separation performance and reproducibility of electrophoresis data.

(d) Means for solving the problem This invention is a cooling device for electrophoresis, which consists of an insulating layer with thermal conductivity, a metal plate layer with high thermal conductivity, and an insulating layer that reinforces both layers. It is provided with a laminated plate formed by laminating reinforcing support layers in this order for support, and a cooling means for directly cooling the metal plate layer from the surface on the side of the reinforcing support layer.

(E) Function This device conducts the Joule heat generated during electrophoresis from the insulating layer side of the metal plate layer to the reinforcing support layer side through the metal plate layer, and uses the cooling means to conduct the Joule heat. It is designed so that it can be removed.

(f) Examples This invention will be explained in detail with reference to examples shown in the figures below.

As shown in Figure 1, the electrophoresis cooling device is
It is composed of a laminated plate 1 and a cooling means 2.

As shown in FIG. 2, the laminate 1 includes an insulating layer 3 having thermal conductivity and a metal plate layer 4 having high thermal conductivity.
and a reinforcing support layer 5 that reinforces and supports the two layers 3 and 4 are laminated in this order.
The insulating layer 3 is made of, for example, polyimide film or epoxy resin, and its thickness is preferably 0.1 mm to 0.5 mm, particularly preferably 0.2 mm. The metal plate layer 4 is made of, for example, a flat plate of copper or aluminum, and its thickness is preferably 0.1 mm to 1.0 mm, particularly preferably 0.5 mm to 0.2 mm. The reinforcing support layer 5 is made of, for example, glass fiber reinforced plastic (FRP) and has a thickness of
3.0 mm to 10.0 mm is preferred, particularly 3.0 mm to 5.0 mm.

The cooling means 2, as shown in FIGS. 1 to 3,
It consists of a circulation path 6 for circulating cooling water from outside the laminate 1 into the reinforcing support layer 5, and a cooling water supply device 7 outside the laminate 1 for supplying cooling water to the circulation path 6. As shown in FIGS. 2 and 3, the circulation path 6 consists of six outgoing paths 6a and six incoming paths 6b, which are provided in close contact with the surface of the metal plate layer 4 on the reinforcing support layer 5 side.
It is made up of metal pipes. This metal pipe is made of copper, for example, and its inner diameter is 1.0mm to 5.0mm.
φ is preferable, and 1.5 mmφ is particularly preferable. In addition,
An epoxy resin 8 is filled between the outgoing path 6a and the incoming path 6b of the circulation path 6 for reinforcement. On the other hand, the cooling water supply device is a known device and includes a pump section and a cooling section (not shown).

The above cooling device performs electrophoresis by bringing a migration gel (not shown) into close contact with the surface of the insulating layer 3, and the Joule heat generated at that time is transferred to the reinforcing support layer of the metal plate layer 4 through the insulating layer 3. It is transmitted to the 5th side surface. This conducted Joule heat is discharged from the entire surface of the metal plate layer 4 to the outside of the laminate 1 by the cooling water flowing through the circulation path 6.

Therefore, the electrophoresis gel during electrophoresis can be cooled to a constant temperature, and the temperature distribution over the entire surface of the electrophoresis gel can be made uniform. This makes it possible to improve separation performance and reproducibility of electrophoresis data. In addition, the laminate 1 may be, for example,
Since it can be made as thin as 3.0mm to 5.0mm, it can be used in a variety of applications.

In addition to the above-mentioned embodiments, the cooling means used in this invention also includes a cooling means that circulates a refrigerant such as Freon gas in the circulation path 1.
In addition, a Peltier element (thermo module) closely attached to the surface of the metal plate layer 4 on the side of the reinforcing support layer 5 may be used to radiate the heat absorbed by the Peltier element. It may also be configured with heat dissipation fins.

(g) Effects of the invention According to this invention, the temperature of the electrophoresis gel can be kept constant, and the temperature distribution over the entire surface of the electrophoresis gel can be made uniform. Therefore, separation ability and reproducibility of electrophoresis data can be improved.

[Brief explanation of the drawing]

FIG. 1 is a perspective view showing an embodiment of this invention, FIG. 2 is a sectional view taken along the Z-Z line in FIG. 1, and FIG. 3 is an explanatory plan view illustrating a circulation path used in this invention. DESCRIPTION OF SYMBOLS 1... Laminate board, 2... Cooling means, 3... Insulating layer, 4... Metal plate layer, 5... Reinforcement support layer.

Claims (1)

[Claims for Utility Model Registration] 1. Formed by laminating in this order an insulating layer having thermal conductivity, a metal plate layer having high thermal conductivity, and a reinforcing support layer having insulating properties for reinforcing and supporting both layers. 1. A cooling device for electrophoresis, comprising: a laminated plate made of aluminum, and a cooling means for directly cooling the metal plate layer from its reinforcing support layer side. 2. The cooling means is provided in the reinforcing support layer in close contact with the surface of the metal plate layer on the reinforcing support layer side, and has both ends open to the outside of the laminate, and a cooling means for supplying refrigerant to this refrigerant circulation path. A cooling device for electrophoresis according to claim 1, comprising a refrigerant supply device. 3. The electrophoresis cooling device according to claim 1 or 2, wherein the insulating layer has a thickness of 0.1 mm to 0.5 mm.