JPH044879B2 - - Google Patents
Info
- Publication number
- JPH044879B2 JPH044879B2 JP62173880A JP17388087A JPH044879B2 JP H044879 B2 JPH044879 B2 JP H044879B2 JP 62173880 A JP62173880 A JP 62173880A JP 17388087 A JP17388087 A JP 17388087A JP H044879 B2 JPH044879 B2 JP H044879B2
- Authority
- JP
- Japan
- Prior art keywords
- red
- ions
- yield
- medium
- safflower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000001054 red pigment Substances 0.000 claims description 23
- -1 ammonium ions Chemical class 0.000 claims description 20
- 244000020518 Carthamus tinctorius Species 0.000 claims description 17
- 235000003255 Carthamus tinctorius Nutrition 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 9
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 9
- 229910001424 calcium ion Inorganic materials 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 9
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000049 pigment Substances 0.000 claims description 7
- 230000001737 promoting effect Effects 0.000 claims description 6
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 235000010980 cellulose Nutrition 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 4
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 4
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 4
- 229920002101 Chitin Polymers 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000002609 medium Substances 0.000 description 23
- 206010020649 Hyperkeratosis Diseases 0.000 description 13
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- COLNVLDHVKWLRT-MRVPVSSYSA-N D-phenylalanine Chemical compound OC(=O)[C@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-MRVPVSSYSA-N 0.000 description 3
- 229930182832 D-phenylalanine Natural products 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- WLYGSPLCNKYESI-RSUQVHIMSA-N Carthamin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@]1(O)C(O)=C(C(=O)\C=C\C=2C=CC(O)=CC=2)C(=O)C(\C=C\2C([C@](O)([C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(O)=C(C(=O)\C=C\C=3C=CC(O)=CC=3)C/2=O)=O)=C1O WLYGSPLCNKYESI-RSUQVHIMSA-N 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 229930182827 D-tryptophan Natural products 0.000 description 1
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 description 1
- OUYCCCASQSFEME-MRVPVSSYSA-N D-tyrosine Chemical compound OC(=O)[C@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-MRVPVSSYSA-N 0.000 description 1
- 229930195709 D-tyrosine Natural products 0.000 description 1
- 244000181025 Rosa gallica Species 0.000 description 1
- 235000000533 Rosa gallica Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
（産業上の利用分野）
本発明は、ベニ花の紅色色素を増収する方法に
関するものである。
更に詳細には、本発明は、ベニ花のカルスをア
ンモニウムイオン及び／又はカルシウムイオン及
び／又はマグネシウムイオンを除き、Ｄ体及び／
又はDL体の芳香族アミノ酸を添加した液体培地
で培養することによつてベニ花の紅色色素を増収
する方法に関するものである。
本発明によつて天然色素であるベニ花の紅色色
素を多量生産できるようになるため、化粧品業
界、食品業界等天然色素を利用する業界に益する
ところ大なるものがある。
（従来の技術）
一般に、ベニバナは秋田地方でよく栽培されて
いる菊科の植物で、収穫される花は美しい紅色色
素（カルタミン）、黄色色素等を含み、また、そ
の他漢方的薬効成分も含むために、乾燥した花は
お茶として珍重されている。また、花から抽出し
た紅色色素は紅ぞめ染料として、また、天然の口
紅として販売されている。
ベニバナに含まれる紅色色素は天然色素として
きわめて有用であるところから、本発明者らは、
先に、ベニバナの紅色色素を大量生産するために
ベニバナのカルス培養について鋭意研究したとこ
ろ、ベニバナの細胞を固体培養と液体培養の二段
階培養において、その少くとも１成分の濃度を低
下させることによつて、紅色に着色したカルスを
生産することに成功したのである。
しかしながら、この方法によつても紅色色素の
生成量は少く、大量生産できるまでには至つてい
ない。
（解決しようとする問題点）
ベニ花のカルスの培養液による培養によつて得
られる紅色色素はきわめて微量であるために工業
的に大量生産する段階に至つていない。本発明は
液体培養法の改良によつて、紅色色素の収率を高
めることによつて、ベニ花紅色色素の工業的大量
生産を可能とするものである。
（問題点を解決するための手段）
本発明者らは、ベニバナの紅色色素を大量生産
する方法を求めて研究したところ、アンモニウム
イオン及び／又はカルシウムイオン及び／又はマ
グネシウムイオンを除き、Ｄ体及び／又はDL体
の芳香族アミノ酸もしくはその含有物を添加した
液体培地でベニ花の細胞群を培養することによつ
て紅色色素の収率を高めることができた。
また、培養に際し、セルロースパウダー、微結
晶セルロース、瀘紙、セルロース、キチン、木
綿、絹から選択された発色促進物質を添加してお
けば、紅色色素の収率はより高まることも確認さ
れた。
本発明はアンモニウムイオン及び／又はカルシ
ウムイオン及び／又はマグネシウムイオンを除
き、Ｄ体及び／又はDL体の芳香族アミノ酸もし
くはその含有物を添加した液体培地でベニ花の細
胞群を培養することを特徴とするベニ花紅色色素
の新増収方法である。また、本発明は、アンモニ
ウムイオン及び／又はカルシウムイオン及び／又
はマグネシウムイオンを除き、Ｄ体及び／又は
DL体の芳香族アミノ酸もしくはその含有物及び
発色促進物質を添加した液体培地でベニ花の細胞
群を培養することを特徴とするベニ花紅色色素の
新増収方法である。
本発明で使用するベニバナの細胞群はその幼植
物の葉、茎の組織などから採取されるが、花芽か
ら採取することもできる。ここでいう花芽とはベ
ニバナ植物の葉腋に生ずる将来花となる能力を有
する幼組織である。これは頂上の蕾がなくなつた
時自ら花となる能力を有するものである。
本発明においては、ベニバナの細胞群を最初固
体培地で培養し、得られたカルスをアンモニウム
イオン及び／又はカルシウムイオン及び／又はマ
グネシウムイオンを除き、Ｄ体及び／又はDL体
の芳香族アミノ酸もしくはその含有物を添加した
液体培地で培養する。この培養は発色させるとき
のみの培養で十分である。また、この培養に際
し、最初からまたは途中から発色促進物質を添加
することができる。
一般に、植物組織培養に用いられるムラシゲ・
スクーグ培地、ホワイト培地、ガンボルグ培地等
にはアンモニウムイオン、カルシウムイオン、マ
グネシウムイオンは必ず添加されているが、本発
明ではこれらのいずれか、好ましくは全部を含有
させない。アンモニウムイオン及び／又はカルシ
ウムイオン及び／又はマグネシウムイオンが存在
することによつて紅色色素の生成が著じるしく低
減されるのである。
また、Ｄ体の芳香族アミノ酸としてはＤ−フエ
ニルアラニン、Ｄ−チロシン、Ｄ−トリプトフア
ンなどがあるが、これらが添加されることによつ
て紅色色素の生産は高まるのである。Ｄ体の芳香
族アミノ酸は一般に合成されたときはDL体の混
合物であるが、本発明ではＤ体を含んでいれば
DL体の混合物でもよく、又、DL体単独の芳香族
アミノ酸でもよく、同程度の効果が得られる。
Ｄ体及び／又はDL体の芳香族アミノ酸として
は、液体培地に0.1ｍＭ〜10ｍＭ程度添加される
のがよい。この範囲をはずれると紅色色素の生産
はむしろ低下する傾向となる。
また、発色促進物質としてはセルロースパウダ
ー、微結晶セルロース、瀘紙、セルロース、キチ
ン、木綿、絹から選択された１つ以上があげられ
るが、紅色色素の生産を促進する物質であればよ
く、液体培地に0.1〜10％、好ましくは１〜５％
程度添加されるのがよい。培養が終了し、紅色に
なつたセルロースパウダー、微結晶セルロース、
セルロース等の発色促進物質からはピリジン等の
有機溶剤で紅色色素を容易に分離することがで
き、美しい紅色色素を単離することができるもの
である。
次に本発明の実施例を示すが、ここで用いたム
ラシゲ・スクーグの改良培地として、対照培地の
KBC−G1及び本発明における改良培地のKBC−
P5、KBC−P1、KBC−P2及びKBC−P3の各組
成を次の表１に示す。
(Industrial Application Field) The present invention relates to a method for increasing the yield of red pigment of safflower. More specifically, the present invention removes ammonium ions and/or calcium ions and/or magnesium ions from the callus of safflower and converts it into D-form and/or
Alternatively, the present invention relates to a method for increasing the yield of the red pigment of safflower by culturing it in a liquid medium supplemented with a DL aromatic amino acid. The present invention makes it possible to produce a large amount of the natural red pigment of safflower, which will greatly benefit industries that use natural pigments, such as the cosmetics industry and the food industry. (Prior art) Generally, safflower is a plant of the Chrysanthemum family that is commonly cultivated in the Akita region, and the flowers that are harvested contain beautiful red pigments (carthamine), yellow pigments, etc., and also contain other Chinese herbal medicinal ingredients. For this reason, the dried flowers are prized as tea. In addition, the red pigment extracted from the flower is sold as red dye and as a natural lipstick. Since the red pigment contained in safflower is extremely useful as a natural pigment, the present inventors
Previously, in order to mass-produce safflower's red pigment, we conducted intensive research on safflower callus culture, and found that we could reduce the concentration of at least one component of safflower cells in two-stage culture of solid culture and liquid culture. As a result, they succeeded in producing callus that was colored red. However, even with this method, the amount of red pigment produced is small, and mass production has not yet been possible. (Problem to be Solved) The red pigment obtained by culturing the callus of the red flower in a culture solution is extremely small, so it has not yet reached the stage of industrial mass production. The present invention improves the liquid culture method to increase the yield of the red pigment, thereby making possible industrial mass production of the red red pigment. (Means for Solving the Problems) The present inventors conducted research to find a method for mass-producing the red pigment of safflower, and found that, except for ammonium ions and/or calcium ions and/or magnesium ions, D-form and The yield of red pigment could be increased by culturing a group of cells of safflower in a liquid medium supplemented with/or a DL aromatic amino acid or a substance containing it. It was also confirmed that the yield of red pigment could be further increased if a color-promoting substance selected from cellulose powder, microcrystalline cellulose, filter paper, cellulose, chitin, cotton, and silk was added during culture. The present invention is characterized in that a group of cells of safflower is cultured in a liquid medium to which ammonium ions and/or calcium ions and/or magnesium ions are removed and D- and/or DL-form aromatic amino acids or their contents are added. This is a new method to increase the yield of safflower pigment. In addition, the present invention does not include ammonium ions and/or calcium ions and/or magnesium ions;
This is a new method for increasing the yield of red red pigment of red rose flower, which is characterized by culturing a group of cells of red flower red flower in a liquid medium to which a DL aromatic amino acid or its content and a color development promoting substance are added. The safflower cells used in the present invention are collected from leaves, stem tissues, etc. of young plants, but they can also be collected from flower buds. The flower bud referred to here is a young tissue that develops in the leaf axils of a safflower plant and has the ability to become a flower in the future. This has the ability to turn into a flower by itself when the top bud dies. In the present invention, a group of safflower cells is first cultured in a solid medium, and ammonium ions and/or calcium ions and/or magnesium ions are removed from the resulting callus, and D- and/or DL-form aromatic amino acids or their Culture in a liquid medium supplemented with ingredients. It is sufficient to carry out this culture only for color development. Further, during this culture, a color development promoting substance can be added from the beginning or during the culture. Commonly used for plant tissue culture
Ammonium ions, calcium ions, and magnesium ions are always added to Skoog's medium, White's medium, Gamborg's medium, etc., but in the present invention, any of these, preferably not all, are added. The presence of ammonium ions and/or calcium ions and/or magnesium ions significantly reduces the formation of red pigment. Further, D-type aromatic amino acids include D-phenylalanine, D-tyrosine, D-tryptophan, etc., and the production of red pigment is increased by adding these. When D-form aromatic amino acids are synthesized, they are generally a mixture of DL-forms, but in the present invention, if they contain D-forms,
A mixture of DL forms or a single aromatic amino acid of DL form may be used, and similar effects can be obtained. The D-form and/or DL-form aromatic amino acid is preferably added to the liquid medium in an amount of about 0.1 mM to 10 mM. Outside this range, the production of red pigment tends to decrease. In addition, as the color development promoting substance, one or more selected from cellulose powder, microcrystalline cellulose, filter paper, cellulose, chitin, cotton, and silk can be mentioned, but any substance that promotes the production of red pigment may be used. 0.1-10%, preferably 1-5% in the medium
It is best to add some amount. Cellulose powder, microcrystalline cellulose, which has turned red after culturing,
The red pigment can be easily separated from a color development promoting substance such as cellulose using an organic solvent such as pyridine, and a beautiful red pigment can be isolated. Next, an example of the present invention will be shown, in which the improved Murashige-Skoog medium used here was a control medium.
KBC-G1 and KBC- of the improved medium of the present invention
The compositions of P5, KBC-P1, KBC-P2 and KBC-P3 are shown in Table 1 below.
【表】
試験例
300ml三角フラスコにKBC−G1培地を75ml入
れ、120℃10分間減菌した。冷却後、ベニ花の湿
潤カルス3.5ｇを入れ25℃で３日間旋回培養した。
約２倍に増殖した細胞を吸引濾過により取得し同
上のKBC−G1培地に同様にして3.5ｇ入れさらに
３日間培養する。この操作をくり返し活発な増殖
カルスを得る。
得られた増殖カルス3.5ｇを、Ｄ−フエニルア
ラニン１ｍＭ及びセルロース・パウダー４％添加
したKBC−G1培地で、
１ NH4NO3、CaCl2・2H2O及びMgSO4・
7H2Oを除いた培地
２ NH4NO3及びCaCl2・2H2Oを除いた培地
３ NH4NO3及びMgSO4・7H2Oを除いた培地
４ NH4NO3、CaCl2・2H2O及びMgSO4・
7H2Oをそのまま含む培地
に分けて３日間25℃で旋回培養し、培養後カルス
と着色したセルロース・パウダーを分離し、色素
をピリジンで溶出し、その520nｍの吸光度で発
色度を測定し、次の結果を得た。
試験培養
培地 発色度(A520)
１ 1.76
２ 0.65
３ 1.75
４ 0.15
実験例
300ml三角フラスコにKBC−G1培地を75ml入
れ、120℃10分間滅菌した。冷却後、ベニ花の湿
潤カルス3.5ｇを入れ25℃で３日間旋回培養した。
約２倍に増殖した細胞を吸引濾過により取得し同
上のKBC−G1培地に同様にして3.5ｇ入れさらに
３日間培養する。この操作をくり返し活発な増殖
カルスを得る。得られた増殖カルス3.5ｇをKBC
−P1〜KBC−P5それぞれの培地に入れ３日間25
℃で旋回培養した。
なお、本実施例ではＤ−フエニルアラニン１ｍ
Ｍ及びセルロースパウダー４％を添加した。
培養後カルスと着色したセルロースパウダーを
分離し、吸着した色素をピリジンで溶出し、その
520nｍの吸光度で発色度とした。その結果は次
の表２に示される。[Table] Test Example 75 ml of KBC-G1 medium was placed in a 300 ml Erlenmeyer flask and sterilized at 120°C for 10 minutes. After cooling, 3.5 g of wet callus of Redflower was added and cultured with rotation at 25°C for 3 days.
Cells that have grown approximately twice as much are obtained by suction filtration, and 3.5 g of the cells are added to the same KBC-G1 medium as above and cultured for an additional 3 days. This operation is repeated to obtain actively proliferating callus. 3.5 g of the obtained proliferated callus was cultured in KBC-G1 medium supplemented with 1 mM D-phenylalanine and 4% cellulose powder, containing 1 NH 4 NO 3 , CaCl 2 .2H 2 O and MgSO 4 .
Medium 2 excluding 7H 2 O Medium 3 excluding NH 4 NO 3 and CaCl 2・2H 2 O Medium 4 excluding NH 4 NO 3 and MgSO 4・7H 2 O NH 4 NO 3 , CaCl 2・2H 2 O and MgSO4・
Divided into a medium containing 7H 2 O as it is and cultured with rotation at 25°C for 3 days. After culturing, the callus and colored cellulose powder were separated, the pigment was eluted with pyridine, and the degree of color development was measured by its absorbance at 520 nm. I got the following results. Test culture medium color development (A 520 ) 1 1.76 2 0.65 3 1.75 4 0.15 Experimental example 75 ml of KBC-G1 medium was placed in a 300 ml Erlenmeyer flask and sterilized at 120°C for 10 minutes. After cooling, 3.5 g of wet callus of Redflower was added and cultured with rotation at 25°C for 3 days.
Cells that have grown approximately twice as much are obtained by suction filtration, and 3.5 g of the cells are added to the same KBC-G1 medium as above and cultured for an additional 3 days. This operation is repeated to obtain actively proliferating callus. 3.5g of the obtained proliferated callus was converted into KBC.
- P1 ~ KBC - P5 each culture medium for 3 days 25
The cells were incubated with rotation at ℃. In addition, in this example, 1 m of D-phenylalanine
M and 4% cellulose powder were added. After culturing, the callus and colored cellulose powder are separated, and the adsorbed pigment is eluted with pyridine.
The degree of color development was determined by the absorbance at 520 nm. The results are shown in Table 2 below.
Claims (1)
ムイオン及び／又はマグネシウムイオンを除き、
Ｄ体及び／又はDL体の芳香族アミノ酸もしくは
その含有物を添加した液体培地でベニ花の細胞群
を培養することを特徴とするベニ花紅色色素の新
増収方法。 ２ アンモニウムイオンを除き、そしてカルシウ
ムイオン及び／又はマグネシウムイオンを除き、
Ｄ体及び／又はDL体の芳香族アミノ酸もしくは
その含有物及び発色促進物質を添加した液体培地
でベニ花の細胞群を培養することを特徴とするベ
ニ花紅色色素の新増収方法。 ３ 発色促進物質がセルロースパウダー、微結晶
セルロース、瀘紙、セルロース、キチン、木綿、
絹から選択された１つ以上である特許請求の範囲
第２項記載のベニ花紅色色素の新増収方法。[Claims] 1. Excluding ammonium ions and excluding calcium ions and/or magnesium ions,
1. A new method for increasing the yield of red red pigment of red flowers, which comprises culturing a cell group of red flowers in a liquid medium supplemented with D- and/or DL-type aromatic amino acids or substances containing them. 2 excluding ammonium ions and excluding calcium ions and/or magnesium ions,
1. A new method for increasing the yield of a red pigment of a red flower, which comprises culturing a cell group of a red flower in a liquid medium to which a D-form and/or a DL-form aromatic amino acid or its content and a color development promoting substance are added. 3 Color development promoting substances include cellulose powder, microcrystalline cellulose, filter paper, cellulose, chitin, cotton,
A new method for increasing the yield of safflower pigment according to claim 2, which is one or more pigments selected from silk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62173880A JPS6420092A (en) | 1987-07-14 | 1987-07-14 | Novel yield increase of red dyestuff of safflower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62173880A JPS6420092A (en) | 1987-07-14 | 1987-07-14 | Novel yield increase of red dyestuff of safflower |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6420092A JPS6420092A (en) | 1989-01-24 |
| JPH044879B2 true JPH044879B2 (en) | 1992-01-29 |
Family
ID=15968830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62173880A Granted JPS6420092A (en) | 1987-07-14 | 1987-07-14 | Novel yield increase of red dyestuff of safflower |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6420092A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020203947A1 (en) * | 2019-03-29 | 2020-10-08 | キリンホールディングス株式会社 | Plant accumulating useful substance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63199766A (en) * | 1987-02-17 | 1988-08-18 | Kibun Kk | Method of increasing yield of red pigment of safflower |
-
1987
- 1987-07-14 JP JP62173880A patent/JPS6420092A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63199766A (en) * | 1987-02-17 | 1988-08-18 | Kibun Kk | Method of increasing yield of red pigment of safflower |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6420092A (en) | 1989-01-24 |
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