JPS62257384A - Method for tissue culture for producing yellow dyestuff of safflower - Google Patents
Method for tissue culture for producing yellow dyestuff of safflowerInfo
- Publication number
- JPS62257384A JPS62257384A JP61097859A JP9785986A JPS62257384A JP S62257384 A JPS62257384 A JP S62257384A JP 61097859 A JP61097859 A JP 61097859A JP 9785986 A JP9785986 A JP 9785986A JP S62257384 A JPS62257384 A JP S62257384A
- Authority
- JP
- Japan
- Prior art keywords
- safflower
- callus
- medium
- yellow dyestuff
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000020518 Carthamus tinctorius Species 0.000 title claims abstract description 29
- 235000003255 Carthamus tinctorius Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 5
- 239000000975 dye Substances 0.000 title abstract 4
- 239000001052 yellow pigment Substances 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 6
- 206010020649 Hyperkeratosis Diseases 0.000 abstract description 24
- 229920001817 Agar Polymers 0.000 abstract description 7
- 239000008272 agar Substances 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 7
- 229930192334 Auxin Natural products 0.000 abstract description 3
- 239000002363 auxin Substances 0.000 abstract description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract 2
- LNETULKMXZVUST-UHFFFAOYSA-N 1-naphthoic acid Chemical compound C1=CC=C2C(C(=O)O)=CC=CC2=C1 LNETULKMXZVUST-UHFFFAOYSA-N 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- -1 sucrose Chemical class 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 239000001054 red pigment Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- KINGXFAMZNIVNL-SXQDSXCISA-N safflor yellow A Natural products OC[C@@H]1O[C@H]2[C@H](OC3=C2C(=O)C(=C(O)C=Cc4ccc(O)cc4)C(=O)[C@]3(O)[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H](O)[C@H]1O KINGXFAMZNIVNL-SXQDSXCISA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- WLYGSPLCNKYESI-RSUQVHIMSA-N Carthamin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@]1(O)C(O)=C(C(=O)\C=C\C=2C=CC(O)=CC=2)C(=O)C(\C=C\2C([C@](O)([C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(O)=C(C(=O)\C=C\C=3C=CC(O)=CC=3)C/2=O)=O)=C1O WLYGSPLCNKYESI-RSUQVHIMSA-N 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000000528 Ricinus communis Species 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000020197 coconut milk Nutrition 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- ZDGGJQMSELMHLK-UHFFFAOYSA-N m-Trifluoromethylhippuric acid Chemical compound OC(=O)CNC(=O)C1=CC=CC(C(F)(F)F)=C1 ZDGGJQMSELMHLK-UHFFFAOYSA-N 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、ベニバナの黄色色素を著量含有する組織培養
物を培養によって製造する方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing, by culturing, a tissue culture containing a significant amount of safflower yellow pigment.
更に詳細には、本発明は、ベニバナの花芽を用いること
によって黄色色素を含有したベニバナのカルスを著量製
造する方法に関するものである。More specifically, the present invention relates to a method for producing large quantities of safflower callus containing yellow pigment by using safflower flower buds.
一般に、ベニバナは秋田地方でよく栽培されている菊科
の植物で、収穫される花は美しい紅色色素（カルタミン
）のほかに黄色色素を含み、また、その他漢方的薬効成
分も含むために、乾燥した花はお茶としてちんちょうさ
れている。また、花から抽出した紅色色素は紅ぞめ染料
として、また、天然の口紅として販売されている。この
紅色色素には黄色色素も混合されている。In general, safflower is a plant of the Chrysanthemum family that is commonly cultivated in the Akita region.The flowers that are harvested contain a beautiful red pigment (carthamine) as well as a yellow pigment, as well as other Chinese medicinal ingredients. The flowers are made into tea. In addition, the red pigment extracted from the flower is sold as red dye and as a natural lipstick. This red pigment is also mixed with a yellow pigment.
有用なベニバナの黄色色素を大量生産するには、ベニバ
ナそのものを大量に栽培し、花から黄色色素のみを分離
すればよいのであるが、ベニバナの花を咲かせるまでに
は時間がかかり、また、花の良否が天候に左右されるな
どの問題があり、その価格も高いものとなっているので
ある。In order to mass-produce the useful yellow pigment of safflower, it is sufficient to cultivate a large amount of safflower itself and separate only the yellow pigment from the flowers, but it takes time for safflower to bloom, and There are problems such as the quality of the product being affected by the weather, and the price is high.
そこで、このように有用なベニバナの黄色色素を、未分
化の細胞群（カルス）を利用して生産することも考えら
れるのであるが、従来、ベニバナの黄色色素のカルス培
養によって生産した例はみられない。Therefore, it is possible to produce the useful yellow pigment of safflower using undifferentiated cell groups (callus), but until now there have been no examples of producing yellow pigment of safflower by callus culture. I can't.
本発明者らは、ベニバナの黄色色素を大量生産するため
にベニバナのカルス培養について鋭意研究したところ、
ベニバナの花芽から分離した細胞もしくは細胞群を培養
することによって黄色に着色したカルスを多量生産する
ことに成功したのである。The present inventors conducted intensive research on safflower callus culture in order to mass-produce yellow safflower pigment.
By culturing cells or cell groups isolated from safflower flower buds, they succeeded in producing large quantities of yellow-colored callus.
本発明は、ベニバナの黄色色素生産組織培養において、
ベニバナの花芽を用いることを特徴とするベニバナの黄
色色素生産組織培養法である。The present invention provides yellow pigment-producing tissue culture of safflower,
This is a safflower yellow pigment producing tissue culture method characterized by using safflower flower buds.
本発明で使用するベニバナの花芽とはベニバナ植物が成
長して頂上に蕾をつけた后頂上の蕾より下位にある葉の
葉液に生ずる未分化又は分化直前の幼組織をいう。これ
は頂上の蕾がなくなった時自ら花となる能力を有するも
のである。The flower bud of safflower used in the present invention refers to a young tissue that is undifferentiated or just before differentiation that occurs in the leaf sap of a leaf below the bud at the top after a safflower plant has grown and has a bud at the top. This has the ability to turn into a flower by itself when the top bud is gone.
ベニバナの花芽から分離した細胞もしくは細胞群は先ず
寒天培地上で、次いで液体培地で増殖させることによっ
て黄色色素を含有する大量のカルスを得ることができる
。A large amount of callus containing yellow pigment can be obtained by first growing cells or cell groups isolated from safflower flower buds on an agar medium and then in a liquid medium.
本発明で用いる寒天培地、液体培地には、いずれにもオ
ーキシン作用物質を添加するのがよい。It is preferable to add an auxin-active substance to both the agar medium and the liquid medium used in the present invention.
オーキシン作用物質としてはナフタレン酢酸（ＮＡＡ）
がよく、その濃度は１０−４〜１．０−１１Ｍ程度でよ
い。Naphthalene acetic acid (NAA) is an auxin agent.
The concentration may be about 10-4 to 1.0-11M.
培地の他の成分については通常の植物組織培養の培地を
用いればよく、特に制限はない。Regarding the other components of the medium, a normal plant tissue culture medium may be used, and there are no particular limitations.
即ち炭素源、エネルギー源としてはショ糖等の炭水化物
、その誘導体、脂肪酸等の有機酸、エタノール等の一級
アルコール、アスパラギン酸等のアミノ酸などが例示さ
れ、無機塩としては塩化カルシウム、硫酸マグネシウム
、硫酸鉄、リン酸二水素カルシウム等が例示される。That is, examples of carbon sources and energy sources include carbohydrates such as sucrose, derivatives thereof, organic acids such as fatty acids, primary alcohols such as ethanol, and amino acids such as aspartic acid, and examples of inorganic salts include calcium chloride, magnesium sulfate, and sulfuric acid. Examples include iron, calcium dihydrogen phosphate, and the like.
又、窒素源としてアンモニウム・イオン、硝酸イオン、
アミノ酸、ペプトンのようなタンパク質の分解物等の窒
素含有化合物が例示される。In addition, ammonium ions, nitrate ions,
Examples include nitrogen-containing compounds such as amino acids and protein decomposition products such as peptone.
液体培地として具体的にはムラシゲ・スクーグの培地、
ホワイトの培地、ガンボルグの培地およびこれらの改変
培地がある。その他培地中にカイネチン、ゼアチン、ベ
ンジルアデニン等のサイトカイニンを０．１〜１００μ
Ｍ、特ニ１０−’ 〜１０−７Ｍ（７）１％度に液体培
地中に共存させておくと、カルスの生育色素の生成に良
好である。又必要に応じイーストエキス、麦芽エキス、
ヒマ１−汁、カザミノ酸、ココナツミルク、ビタミン混
合物等の栄養物を添加してもよい。Specifically, the liquid medium is Murashige-Skoog medium,
There are White's medium, Gamborg's medium and modified mediums thereof. Add 0.1 to 100μ of other cytokinins such as kinetin, zeatin, and benzyladenine to the medium.
M, especially D10-' to 10-7M (7) When allowed to coexist in a liquid medium at a concentration of 1%, it is good for the production of callus growth pigment. In addition, yeast extract, malt extract,
Nutrients such as castor bean juice, casamino acids, coconut milk, vitamin mixtures, etc. may be added.
また、固体培地としては、上述の液体培地に０．８％程
度の寒天を添加するだけのもので十分である。Further, as a solid medium, it is sufficient to add about 0.8% agar to the above-mentioned liquid medium.
本発明の組織培養方法の好適例としては以下のような方
法がある。即ち、ベニバナの花芽の細胞又は細胞群を無
菌的に採取し、ムラシゲ・スクーグの培地の窒素、リン
、カリウムの濃度を１／２とＬ、又ＮＡＡ ヲ１０−’
Ｍ、ヘンシル７テニン（ＢＡ）を１０−’Ｍ とした
寒天培地に置床し、１０〜３５℃で７〜３０日程度培養
し、細胞又は細胞群をカルス化させる。このようにして
得られたカルスを継代培養すると生産速度が漸次高まり
安定化したカルスが得られる。このカルスを同じ組成又
はＮＨ４＋イオンを減少させた液体培地に添加して旋回
培養する。Preferred examples of the tissue culture method of the present invention include the following methods. That is, safflower flower bud cells or cell groups were collected aseptically, and the nitrogen, phosphorus, and potassium concentrations of Murashige-Skoog medium were reduced to 1/2 and NAA 10-'.
The cells or cell groups are placed on an agar medium containing 10-'M of M, Hensyl 7-tenin (BA) and cultured at 10-35°C for about 7-30 days to form a callus. When the callus thus obtained is subcultured, the production rate gradually increases and stable callus is obtained. This callus is added to a liquid medium of the same composition or with reduced NH4+ ions and cultured with rotation.
本発明の培養においては光は必ずしも必要でなく培養温
度は１０〜３５℃、特に２５℃付近が好適である。In the culture of the present invention, light is not necessarily required, and the culture temperature is preferably 10 to 35°C, particularly around 25°C.
固体培養を経た液体培養によってカルスは黄色となり、
多量の黄色色素が生成しているのが分る。The callus becomes yellow due to liquid culture after solid culture.
It can be seen that a large amount of yellow pigment is produced.
黄色色素をカルスから抽出するには、従来から行なわれ
ているベニバナの色素の抽出方法と同じでよい。Yellow pigment can be extracted from callus by the same method as conventionally used for extracting safflower pigment.
本発明においては、ベニバナの花芽の細胞もしくは細胞
群を用いて組織培養することによって黄色色素を含有し
たカルスを製造することができたものである。In the present invention, callus containing yellow pigment can be produced by tissue culture using cells or cell groups of safflower flower buds.
次に本発明の実施例を示すが、ここで用いた甲培地、乙
培地の各組成を次の表１に示す。Next, examples of the present invention will be shown, and the compositions of medium A and medium O used here are shown in Table 1 below.
表１
実施例１゜
ベニバナの花芽のわずかにふくらんだ時、無菌的に細胞
群を多数分離した。Table 1 Example 1 When the safflower flower buds were slightly swollen, a large number of cell groups were separated aseptically.
別に、表１の甲培地に寒天を添加して製造した固体培地
を用意し、これにベニバナ花芽細胞群を分散して、２５
°Ｃで２０日培養し、多数のカルスを得た。Separately, a solid medium prepared by adding agar to the A medium shown in Table 1 was prepared, and a group of safflower flower bud cells was dispersed therein.
After culturing at °C for 20 days, a large number of calli were obtained.
次に、Ｌｏｏｍ Ｑのエルレンマイヤーフラスコに表１
の乙培地３０ｍ Ｑを入れ、１２０℃、１０分滅菌し、
これに上記のカルス０．７ｇを入れ、２５℃で１４日間
旋回培養した。Next, add Table 1 to the Loom Q Erlenmeyer flask.
Add 30m of Otsu culture medium and sterilize it at 120℃ for 10 minutes.
0.7 g of the above callus was added to this, and cultured with rotation at 25° C. for 14 days.
培養後カルスを濾取し、２４時間風乾して乾燥カルス９
ｇ／ Ｑ培養液を得、これを磨砕し、エタノール抽出
し、エタノールを蒸発させることによって黄色色素を４
抛ｇ／ｇ乾燥カルスを得た。After culturing, the callus was collected by filtration and air-dried for 24 hours to obtain dry callus9.
g/Q culture solution was obtained, which was ground, extracted with ethanol, and the yellow pigment was removed by evaporating the ethanol.
Dry callus (g/g) was obtained.
実施例２゜
ベニバナの花芽をまだ外観では判別できない状態のとき
、そのところを切断し、無菌的に小細胞群を多数分離″
した。Example 2゜When a safflower flower bud is still in a state where it cannot be determined visually, the part is cut and a large number of small cell groups are isolated in a sterile manner.''
did.
別に、表１の甲培地に寒天を添加して製造した固体培地
に上記小細胞群を分散し、２５℃で１５日培養し、多数
のカルスを得た。Separately, the above small cell groups were dispersed in a solid medium prepared by adding agar to the A medium shown in Table 1, and cultured at 25°C for 15 days to obtain a large number of calli.
次に、１０抛Ｑのエルレンマイヤーフラスコに表１の乙
培地３０ｍ Ｑを入れ、１２０℃、１０分滅菌し、これ
に上記のカルス０．７８を入れ、２５℃で１５目間旋回
培養した。Next, 30 mQ of the Otsu medium shown in Table 1 was placed in a 10 ml Erlenmeyer flask and sterilized at 120°C for 10 minutes.The above callus 0.78 was placed in this and cultured by rotation at 25°C for 15 days. .
培養後カルスを濾取し、２４時間風乾して乾燥ヵルス８
ｇ／Ｑ培養液を得、これを磨砕し、エタノール抽出し、
エタノールを蒸発させ、黄色色素を５０ｍｙ、１ｇ乾燥
カルスを得た。After culturing, the callus was collected by filtration, air-dried for 24 hours, and dried callus 8
g/Q culture solution was obtained, which was ground, extracted with ethanol,
Ethanol was evaporated to obtain 50 my of yellow pigment and 1 g of dry callus.
Claims (1)
花芽を用いることを特徴とするベニバナの黄色色素生産
組織培養法。A method for culturing yellow pigment-producing tissues of safflower, which comprises using flower buds of safflower in the tissue culture for producing yellow pigments of safflower.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61097859A JPH064027B2 (en) | 1986-04-30 | 1986-04-30 | Safflower yellow pigment producing tissue culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61097859A JPH064027B2 (en) | 1986-04-30 | 1986-04-30 | Safflower yellow pigment producing tissue culture method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62257384A true JPS62257384A (en) | 1987-11-09 |
| JPH064027B2 JPH064027B2 (en) | 1994-01-19 |
Family
ID=14203475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61097859A Expired - Fee Related JPH064027B2 (en) | 1986-04-30 | 1986-04-30 | Safflower yellow pigment producing tissue culture method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH064027B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001335716A (en) * | 2000-05-26 | 2001-12-04 | Sanei Gen Ffi Inc | Deoderized safflower yellow coloring matter |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6269984A (en) * | 1985-09-20 | 1987-03-31 | Kibun Kk | Method of tissue culture for production of red dyestuff of safflower |
-
1986
- 1986-04-30 JP JP61097859A patent/JPH064027B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6269984A (en) * | 1985-09-20 | 1987-03-31 | Kibun Kk | Method of tissue culture for production of red dyestuff of safflower |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001335716A (en) * | 2000-05-26 | 2001-12-04 | Sanei Gen Ffi Inc | Deoderized safflower yellow coloring matter |
| EP1293540A4 (en) * | 2000-05-26 | 2004-12-08 | San Ei Gen Ffi Inc | Deodorized yellow colorant of safflower |
| JP4510230B2 (en) * | 2000-05-26 | 2010-07-21 | 三栄源エフ・エフ・アイ株式会社 | Deodorized safflower yellow |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH064027B2 (en) | 1994-01-19 |
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