JPH044878B2 - - Google Patents
Info
- Publication number
- JPH044878B2 JPH044878B2 JP3232187A JP3232187A JPH044878B2 JP H044878 B2 JPH044878 B2 JP H044878B2 JP 3232187 A JP3232187 A JP 3232187A JP 3232187 A JP3232187 A JP 3232187A JP H044878 B2 JPH044878 B2 JP H044878B2
- Authority
- JP
- Japan
- Prior art keywords
- red
- medium
- flower
- safflower
- red pigment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000001054 red pigment Substances 0.000 claims description 22
- -1 aromatic amino acid Chemical class 0.000 claims description 12
- 239000003463 adsorbent Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 8
- 229910052791 calcium Inorganic materials 0.000 claims description 8
- 239000011575 calcium Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 229910052749 magnesium Inorganic materials 0.000 claims description 8
- 239000011777 magnesium Substances 0.000 claims description 8
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 235000010980 cellulose Nutrition 0.000 claims description 4
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 4
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 4
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 4
- 239000000049 pigment Substances 0.000 claims description 4
- 229920002101 Chitin Polymers 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 2
- 244000181025 Rosa gallica Species 0.000 claims 2
- 235000000533 Rosa gallica Nutrition 0.000 claims 2
- 239000002609 medium Substances 0.000 description 26
- 244000020518 Carthamus tinctorius Species 0.000 description 19
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 18
- 206010020649 Hyperkeratosis Diseases 0.000 description 13
- 238000004519 manufacturing process Methods 0.000 description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000654 additive Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- COLNVLDHVKWLRT-MRVPVSSYSA-N D-phenylalanine Chemical compound OC(=O)[C@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-MRVPVSSYSA-N 0.000 description 2
- 229930182832 D-phenylalanine Natural products 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- WLYGSPLCNKYESI-RSUQVHIMSA-N Carthamin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@]1(O)C(O)=C(C(=O)\C=C\C=2C=CC(O)=CC=2)C(=O)C(\C=C\2C([C@](O)([C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(O)=C(C(=O)\C=C\C=3C=CC(O)=CC=3)C/2=O)=O)=C1O WLYGSPLCNKYESI-RSUQVHIMSA-N 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 229930182827 D-tryptophan Natural products 0.000 description 1
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 description 1
- OUYCCCASQSFEME-MRVPVSSYSA-N D-tyrosine Chemical compound OC(=O)[C@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-MRVPVSSYSA-N 0.000 description 1
- 229930195709 D-tyrosine Natural products 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
（産業上の利用分野）
本発明は、ベニ花の紅色色素を増収する方法に
関するものである。
更に詳細には、本発明は、ベニ花のカルスをカ
ルシウム及び／又はマグネシウムを除き、Ｄ体及
び／又はDL体の芳香族アミノ酸を添加した液体
培地で培養することによつてベニ花の紅色色素を
増収する方法に関するものである。
本発明によつて天然色素であるベニ花の紅色色
素を多量生産できるようになるため、化粧品業
界、食品業界等天然色素を利用する業界に益する
ところ大なるものがある。
（従来の技術）
一般に、ベニバナは秋田地方でよく栽培されて
いる菊科の植物で、収穫される花は美しい紅色色
素（カルタミン）、黄色色素等を含み、また、そ
の他漢方的薬効成分も含むために、乾燥した花は
お茶として珍重されている。また、花から抽出し
た紅色色素は紅ぞめ染料として、また、天然の口
紅として販売されている。
ベニバナに含まれる紅色色素は天然色素として
きわめて有用であるところから、本発明者らは、
先に、ベニバナの紅色色素を大量生産するために
ベニバナのカルス培養について鋭意研究したとこ
ろ、ベニバナの細胞を固体培養と液体培養の二段
階培養において、その少くとも１成分の濃度を低
下させることによつて、紅色に着色したカルスを
生産することに成功したのである。
しかしながら、この方法によつても紅色色素の
生成量は少く、大量生産できるまでには至つてい
ない。
（解決しようとする問題点）
ベニ花のカルスの培養液による培養によつて得
られる紅色色素はきわめて微量であるために工業
的に大量生産する段階に至つていない。本発明は
液体培養法の改良によつて、紅色色素の収率を高
めることによつて、ベニ花紅色色素の工業的大量
生産を可能とするものである。
（問題点を解決するための手段）
本発明者らは、ベニバナの紅色色素を大量生産
する方法を求めて研究したところ、カルシウム及
び／又はマグネシウムを除き、Ｄ体及び／又は
DL体の芳香族アミノ酸もしくはその含有物を添
加した液体培地でベニ花の細胞群を培養すること
によつて紅色色素の収率を高めることができた。
また、培養に際し、キトサン、微結晶セルロー
ス、濾紙、セルロース、キチン、木綿、絹から選
択された吸着剤を添加しておけば、縁色色素の収
率はより高まることも確認された。これら添加物
により同時に色素は吸着されるので本発明ではこ
れら添加物を吸着剤と呼ぶことにする。
本発明はカルシウム及び／又はマグネシウムを
除き、Ｄ体及び／又はDL体の芳香族アミノ酸も
しくはその含有物を添加した液体培地でベニ花の
細胞群を培養することを特徴とするベニ花紅色色
素増収方法である。また、本発明は、カルシウム
及び／又はマグネシウムを除き、Ｄ体及び／又は
DL体の芳香族アミノ酸もしくはその含有物及び
吸着剤を添加した液体培地でベニ花の細胞群を培
養することを特徴とするベニ花紅色色素増収方法
である。
本発明で使用するベニバナの細胞群はその幼植
物の葉、茎の組織などから採取されるが、花芽か
ら採取することもできる。ここでいう花芽とはベ
ニバナ植物の葉腋に生ずる将来花となる能力を有
する幼組織である。これは頂上の蕾がなくなつた
時自ら花となる能力を有するものである。
本発明においては、ベニバナの細胞群を最初固
体培地で培養し、得られたカルスをカルシウム及
び／又はマグネシウムを除き、Ｄ体及び／又は
DL体の芳香族アミノ酸もしくはその含有物を添
加した液体培地で培養する。この培養は発色させ
るときのみの培養で十分である。また、この培養
に際し、最初からまたは途中から吸着剤を添加す
ることができる。
一般に、植物組織培養に用いられるムラシゲ・
スクーグ培地、ホワイト培地、ガンボルグ培地等
にはカルシウム、マグネシウムは必ず添加されて
いるが、本発明ではこれらいずれか、好ましくは
両方を含有させない。カルシウム及び／又はマグ
ネシウムが存在することによつて紅色色素の生成
が著じるしく低減するのである。
また、Ｄ体の芳香族アミノ酸としてはＤ−フエ
ニルアラニン、Ｄ−チロシン、Ｄ−トリプトフア
ンなどがあるが、これらが添加されることによつ
て紅色色素の生産は高まるのである。Ｄ体の芳香
族アミノ酸は一般に合成されたときはDL体の混
合物であるが、本発明ではＤ体を含んでいれば
DL体の混合物でもよく、又、DL体単独の芳香族
アミノ酸でもよく、同程度の効果が得られる。
Ｄ体及び／又はDL体の芳香族アミノ酸として
は、液体培地に10-3w／ｖ％〜10-2w／ｖ％程度
添加されるのがよい。この範囲をはずれると紅色
色素の生産はむしろ低下する傾向となる。
また、吸着剤としてはキトサン、微結晶セルロ
ース、濾紙、セルロース、キチン、木綿、絹から
選択された１つ以上があげられるが、紅色色素を
吸着する物質であればよく、液体培地に0.1〜10
％、好ましくは１〜４％程度添加されるのがよ
い。
培養が終了し、紅色になつた微結晶セルロー
ス、セルロース等の吸着剤からは紅色色素を容易
に分離することができ、美しい紅色色素を単離す
ることができるものである。
次に本発明の実施例を示すが、ここで用いたム
ラシゲ・スクーグの改良培地として甲培地、乙培
地の各組成を次の表１に示す。
(Industrial Application Field) The present invention relates to a method for increasing the yield of red pigment of safflower. More specifically, the present invention aims to cultivate the red pigment of Redflower by culturing the callus of Redflower in a liquid medium excluding calcium and/or magnesium and supplemented with D- and/or DL-type aromatic amino acids. It is about how to increase revenue. The present invention makes it possible to produce a large amount of the natural red pigment of safflower, which will greatly benefit industries that use natural pigments, such as the cosmetics industry and the food industry. (Prior art) Generally, safflower is a plant of the Chrysanthemum family that is commonly cultivated in the Akita region, and the flowers that are harvested contain beautiful red pigments (carthamine), yellow pigments, etc., and also contain other Chinese herbal medicinal ingredients. For this reason, the dried flowers are prized as tea. In addition, the red pigment extracted from the flower is sold as red dye and as a natural lipstick. Since the red pigment contained in safflower is extremely useful as a natural pigment, the present inventors
Previously, in order to mass-produce safflower's red pigment, we conducted intensive research on safflower callus culture, and found that we could reduce the concentration of at least one component of safflower cells in two-stage culture of solid culture and liquid culture. As a result, they succeeded in producing callus that was colored red. However, even with this method, the amount of red pigment produced is small, and mass production has not yet been possible. (Problem to be Solved) The red pigment obtained by culturing the callus of the red flower in a culture solution is extremely small, so it has not yet reached the stage of industrial mass production. The present invention improves the liquid culture method to increase the yield of the red pigment, thereby making possible industrial mass production of the red red pigment. (Means for Solving the Problems) The present inventors conducted research to find a method for mass-producing safflower red pigment, and found that, except for calcium and/or magnesium, D-form and/or
By culturing safflower cells in a liquid medium supplemented with DL aromatic amino acids or their contents, we were able to increase the yield of red pigment. It was also confirmed that the yield of border color pigments can be further increased if an adsorbent selected from chitosan, microcrystalline cellulose, filter paper, cellulose, chitin, cotton, and silk is added during culture. Since the dye is simultaneously adsorbed by these additives, these additives are referred to as adsorbents in the present invention. The present invention is characterized by culturing a group of cells of a safflower in a liquid medium to which calcium and/or magnesium are removed and D- and/or DL-form aromatic amino acids or their contents are added. It's a method. In addition, the present invention provides D-form and/or
This is a method for increasing the yield of red red pigment of a red flower, which is characterized by culturing a group of cells of a red flower in a liquid medium to which a DL aromatic amino acid or its content and an adsorbent are added. The safflower cells used in the present invention are collected from leaves, stem tissues, etc. of young plants, but they can also be collected from flower buds. The flower bud referred to here is a young tissue that develops in the leaf axils of a safflower plant and has the ability to become a flower in the future. This has the ability to turn into a flower by itself when the top bud dies. In the present invention, a group of safflower cells is first cultured in a solid medium, and the resulting callus is freed of calcium and/or magnesium, and then D- and/or
Culture in a liquid medium supplemented with DL aromatic amino acids or their contents. It is sufficient to carry out this culture only for color development. Further, during this culture, an adsorbent can be added from the beginning or during the culture. Commonly used for plant tissue culture
Calcium and magnesium are always added to Skoog's medium, White's medium, Gamborg's medium, etc., but in the present invention, either of these, preferably not both, is added. The presence of calcium and/or magnesium significantly reduces the formation of red pigment. Further, D-type aromatic amino acids include D-phenylalanine, D-tyrosine, D-tryptophan, etc., and the production of red pigment is increased by adding these. When D-form aromatic amino acids are synthesized, they are generally a mixture of DL-forms, but in the present invention, if they contain D-forms,
A mixture of DL forms or a single aromatic amino acid of DL form may be used, and similar effects can be obtained. D- and/or DL-form aromatic amino acids are preferably added to the liquid medium in an amount of about 10 -3 w/v% to 10 -2 w/v%. Outside this range, the production of red pigment tends to decrease. In addition, the adsorbent may be one or more selected from chitosan, microcrystalline cellulose, filter paper, cellulose, chitin, cotton, and silk, but any substance that adsorbs the red pigment may be used, and 0.1 to 10
%, preferably about 1 to 4%. After cultivation, the red pigment can be easily separated from the adsorbent, such as microcrystalline cellulose or cellulose, which has turned red, and a beautiful red pigment can be isolated. Next, Examples of the present invention will be shown, and Table 1 below shows the respective compositions of medium A and medium O as the improved Murashige-Skoog medium used here.
【表】【table】
【表】
試験例
ベニバナの播種後60日目で、花芽のわずかにふ
くらんだ時、無菌的に細胞群を多数分離した。
別に表１の甲培地に寒天9.5ｇ／を添加して
製造した固体培地を用意し、これにベニバナ花芽
細胞群を分散して、25℃で20日培養し、多数のカ
ルスを得た。
300ml三角フラスコに甲培地を75mlを入れ、120
℃15分間滅菌した。冷却後、上述のベニバナの湿
潤カルス3.5ｇを入れ25℃で３日間旋回培養した。
約22倍に増殖した細胞を吸引濾過により取得し同
上の培地に同様にして3.5ｇ入れさらに３日間培
養する。この操作をくり返し活発な増殖カルスを
得る。
得られた増殖カルス3.5ｇを、Ｄ−フエニルア
ラニン５×10-3％及びセルロース・パウダー２％
添加した乙培地で、
１ CaCl2・2H2O及びMgSO4・7H2Oを添加しな
いままの培地
２ CaCl2・2H2Oを440mg／添加した培地
３ MgSO4・7H2Oを370mg／添加した培地
４ CaCl2・2H2O440mg／及びMgSO4・
7H2O370mg／を添加した培地
に分けて３日間25℃でそれぞれ旋回培養し、培養
後カルスとセルロース・パウダーを分離し、後者
の赤色度を色差計により測定し、次の結果を得
た。
試験培養
培地 発赤度(色差計赤色度)
１ 18.6
２ 14.3
３ 5.2
４ 1.2
実施例
ベニバナの播種後60日目で、花芽のわずかにふ
くらんだ時、無菌的に細胞群を多数分離した。
別に表１の甲培地に寒天9.5ｇ／を添加して
製造した固体培地を用意し、これにベニバナ花芽
細胞群を分散して、25℃で20日培養し、多数のカ
ルスを得た。
300ml三角フラスコに甲培地を75ml入れ、120℃
15分間滅菌した。冷却後、上述のベニバナの湿潤
カルス3.5ｇを入れ25℃で３日間旋回培養した。
約２倍に増殖した細胞を吸引濾過により取得し同
上の培地に同様にして3.5ｇ入れさらに３日間培
養する。この操作をくり返し活発な増殖カルスを
得る。
得られた増殖カルス3.5ｇを表２に示す各Ｄ体、
DL体の芳香族アミノ酸を添加した乙培地に吸着
剤（セルロース・パウダー）とともに入れ３日間
25℃で旋回培養した。対照として、無添加の場
合、及びＬ体芳香族アミノ酸の場合も行つた。培
養後カルスと吸着剤（セルロース・パウダー）と
を分離し後者の赤色度を色差計により測定した。
その結果は次の表２に示される。[Table] Test Example On the 60th day after sowing safflower, when the flower buds were slightly swollen, a large number of cell groups were separated aseptically. Separately, a solid medium prepared by adding 9.5 g/agar to the A medium shown in Table 1 was prepared, and safflower flower bud cells were dispersed therein and cultured at 25°C for 20 days to obtain a large number of calli. Pour 75ml of A medium into a 300ml Erlenmeyer flask and add 120ml
Sterilize at 15°C for 15 minutes. After cooling, 3.5 g of the wet safflower callus described above was added and cultured with rotation at 25° C. for 3 days.
Cells that have grown approximately 22 times are obtained by suction filtration, and 3.5 g of cells are similarly added to the same medium and cultured for an additional 3 days. This operation is repeated to obtain actively proliferating callus. 3.5 g of the obtained proliferated callus was mixed with 5 x 10 -3 % D-phenylalanine and 2% cellulose powder.
1 Medium with no addition of CaCl 2 2H 2 O and MgSO 4 7H 2 O 2 Medium with 440 mg/added of CaCl 2 2H 2 O 3 370 mg/addition of MgSO 4 7H 2 O Medium 4 CaCl2・2H2O440mg /and MgSO4・
The cells were divided into medium supplemented with 370 mg of 7H 2 O and cultured with rotation at 25° C. for 3 days. After the culture, the callus and cellulose powder were separated and the redness of the latter was measured using a colorimeter. The following results were obtained. Test culture medium redness (color difference meter redness) 1 18.6 2 14.3 3 5.2 4 1.2 Example On the 60th day after sowing safflower, when the flower buds were slightly swollen, a large number of cell groups were separated aseptically. Separately, a solid medium prepared by adding 9.5 g/agar to the A medium shown in Table 1 was prepared, and safflower flower bud cells were dispersed therein and cultured at 25°C for 20 days to obtain a large number of calli. Pour 75ml of A medium into a 300ml Erlenmeyer flask and heat to 120℃.
Sterilized for 15 minutes. After cooling, 3.5 g of the wet safflower callus described above was added and cultured with rotation at 25° C. for 3 days.
Cells that have grown approximately twice as much are obtained by suction filtration, and 3.5 g of the cells are added to the same medium as above and cultured for an additional 3 days. This operation is repeated to obtain actively proliferating callus. 3.5 g of the obtained proliferated callus was divided into each D form shown in Table 2,
Place in Otsu medium supplemented with DL aromatic amino acids together with adsorbent (cellulose powder) for 3 days.
Rotation culture was performed at 25°C. As a control, a case with no additive and a case with an L-aromatic amino acid were also conducted. After culturing, the callus and the adsorbent (cellulose powder) were separated, and the redness of the latter was measured using a colorimeter.
The results are shown in Table 2 below.
Claims (1)
Ｄ体及び／又はDL体の芳香族アミノ酸もしくは
その含有物を添加した液体培地でベニ花の細胞群
を培養することを特徴とするベニ花紅色色素増収
方法。 ２ カルシウム及び／又はマグネシウムを除き、
Ｄ体及び／又はDL体の芳香族アミノ酸もしくは
その含有物及び吸着剤を添加した液体培地でベニ
花の細胞群を培養することを特徴とするベニ花紅
色色素増収方法。 ３ 吸着剤がキトサン、微結晶セルロース、濾
紙、セルロース、キチン、木綿、絹から選択され
た１つ以上である特許請求の範囲第２項記載のベ
ニ花紅色色素増収方法。[Claims] 1. Excluding calcium and/or magnesium,
1. A method for increasing the yield of red red pigment of a red rose flower, which comprises culturing a cell group of red rose flower in a liquid medium supplemented with a D-form and/or a DL-form aromatic amino acid or a substance containing the same. 2 Excluding calcium and/or magnesium,
1. A method for increasing the yield of red red pigment of a red flower, which comprises culturing a cell group of a red flower in a liquid medium to which a D- and/or DL-type aromatic amino acid or its content and an adsorbent are added. 3. The method for increasing the yield of russet pigment according to claim 2, wherein the adsorbent is one or more selected from chitosan, microcrystalline cellulose, filter paper, cellulose, chitin, cotton, and silk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3232187A JPS63199766A (en) | 1987-02-17 | 1987-02-17 | Method of increasing yield of red pigment of safflower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3232187A JPS63199766A (en) | 1987-02-17 | 1987-02-17 | Method of increasing yield of red pigment of safflower |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63199766A JPS63199766A (en) | 1988-08-18 |
| JPH044878B2 true JPH044878B2 (en) | 1992-01-29 |
Family
ID=12355673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3232187A Granted JPS63199766A (en) | 1987-02-17 | 1987-02-17 | Method of increasing yield of red pigment of safflower |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63199766A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6420092A (en) * | 1987-07-14 | 1989-01-24 | Kibun Kk | Novel yield increase of red dyestuff of safflower |
| JPH0822968B2 (en) * | 1990-09-04 | 1996-03-06 | 株式会社紀文食品 | Dye and its solution |
-
1987
- 1987-02-17 JP JP3232187A patent/JPS63199766A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63199766A (en) | 1988-08-18 |
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