CN100571530C - A kind of preparation method of high purity alpha corn protein - Google Patents
A kind of preparation method of high purity alpha corn protein Download PDFInfo
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- CN100571530C CN100571530C CNB2006100860107A CN200610086010A CN100571530C CN 100571530 C CN100571530 C CN 100571530C CN B2006100860107 A CNB2006100860107 A CN B2006100860107A CN 200610086010 A CN200610086010 A CN 200610086010A CN 100571530 C CN100571530 C CN 100571530C
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Abstract
The invention provides a kind of preparation method of high purity alpha corn protein.Specifically be meant ethanolic solution, adopt counter-current extraction technology, from corn protein powder, extract alpha corn protein with 90%.Regulate temperature, the pH value of alpha corn protein ethanolic solution then, stir behind the adding active carbon, remove pigment.At last with nitrogen as protective medium, make high-purity, high-quality alpha corn protein through closed circuit spray-drying.The alpha corn protein of decolouring and deodorization can be used safely in food and pharmaceutical industries.The alpha corn protein of purifying will have effectiveness and function more widely at existing and potential food and medicine field.
Description
Technical field
The present invention relates to the development and use field of vegetable protein, refering in particular to a kind of is raw material with the corn protein powder, preparation high-purity, high-quality and alpha corn protein method cheaply.It can be widely used in food, medicine and packaging industry.
Background technology
Can obtain a kind of byproduct-corn protein powder CGM (CornGluten Meal) in the process of corn wet production starch, protein content is about 60%.Corn protein powder mainly is to sell as feed at present, and added value is lower.Contain 30～35% the alpha corn protein of having an appointment in the corn protein powder.This albumen is widely used in aspects such as food, papermaking, printing, weaving and medical and health in recent years owing to have good water-fast, resistance oxygen and easy film forming, especially for packaging for foodstuff, is a kind of class coating material that broad prospect of application is arranged.
Owing to exist lutein, carotenoid and other pigment in the corn, they can with hydrophobic combined with protein, form lurid alpha corn protein.When alpha corn protein was applied to food industry, finished product can have offending smell and color and luster, had influence on sense of smell, the visual sensory feature of food.For this reason, the extraction of alpha corn protein and discoloration method have been explored decades (Mason and Palmer, 1934; Swallen, 1938; Pearce, 1941; Starling, 1951).But the effect that up to the present obtains is still undesirable, particularly when its product is used for food industry.
Lawhorn (United States Patent (USP) 4,624,805), Chen and Hoff (United States Patent (USP) 4,716,218), Carter and Reck (United States Patent (USP) 3,535,305) etc. have reported and have adopted ethanol to prepare the method for zein from corn protein powder.Specific implementation method is to adopt ethanol/isopropyl alcohol to extract, and is then that extract is concentrated, dry, obtains product.Yet product all mixes some pigments and other impurity usually, so still need further decolouring and deodorization to handle, can obtain desirable zein product.
Bibliographical information the different solvents discoloration method of zein, as using carrene decolouring (Mason andPalmer, 1934), benzene decolouring (United States Patent (USP) 2,133,591) and some other lower hydrocarbon decolouring (United States Patent (USP) 2,272,488 and 2,229,870).Though above product has minimal color and luster, yet owing to adopt above-mentioned carrene, benzene and acetone and other organic solvent, dissolvent residual has limited these application of decolouring product in food and pharmaceuticals industry.
Above-mentioned report shows, at present method in common all ubiquity contradiction between decolouring and productive rate and the properties of product, wherein decolouring is the principal element that restriction corn protein powder deep process technology develops.The expensive of the existence of corn yellow OB and processing limited the application of zein product in industries such as food and pharmacy, so finds a kind of simple, economical zein extraction, decolouring and deodour method, is one of corn deep processing industry problem demanding prompt solution.
Summary of the invention
The present invention is to provide the technology of preparing of a kind of extraction from corn protein powder, separation, purifying and recovery food-grade/pharmaceutical grade alpha corn protein.
Extract and adopt counter-current extraction techniques, obtain the recovery rate of maximum with the quantity of solvent of minimum.Purifying comprises the impurity of removing in the zein such as smell, color and luster, water-soluble glutelin and starch, specifically is to adopt the suitable surface-active solid absorbent that has that the coloring matter in the solution is adsorbed on the surface of solids, reaches the purpose of decolouring.The material that can be used as adsorbent has: active carbon, Emathlite, silica gel etc.Final extract as protective medium, makes the alpha corn protein of good product performance through closed circuit spray-drying with nitrogen, simultaneously alcohol solvent is reclaimed.
Above-mentioned purpose of the present invention realizes by following technological means.
(1) corn protein powder is granulated, and adds the absolute ethyl alcohol of 5～10 times of volumes then, degreasing 2 times.Adopt vacuum filtration or the centrifugal Separation of Solid and Liquid of carrying out;
(2) step 1 degreasing zein particle being added 4～10 times of volumes, concentration is 80～95% ethanol, room temperature adverse current lixiviate 4～24 hours, Separation of Solid and Liquid;
(3) filtrate of step 2 is regulated pH in 5.5～6.8 scopes, add active carbon or Emathlite then, addition and destainer mass ratio are 1～10%, stir 1～5h under 25～70 ℃ of conditions.The gained mixed liquor adopts separation means such as vacuum filtration, high speed centrifugation or press filtration to carry out Separation of Solid and Liquid.Its filter residue is the processing of can regenerating of active carbon or Emathlite, reuses;
(4) filtrate of step 3 is added the NaCl aqueous solution of 2～4 times of volumes 0.25～1%, gained precipitation water cyclic washing 3 times eliminates NaCl wherein.Carry out vacuum drying then and make alpha corn protein;
(5) with the filtrate of step 3 or be evaporated to 1/2 volume, reclaim ethanol, carry out vacuum drying then and make alpha corn protein;
(6) with the filtrate of step 3 or with nitrogen as protective medium, make high-purity, high-quality alpha corn protein through closed circuit spray-drying.Reclaim ethanol simultaneously.
The used solvent of degreasing in preparation process, decolouring and extraction is the ethanol of food-grade, thereby the alpha corn protein that guarantees decolouring and deodorization can be used safely in food and pharmaceutical industries.The alpha corn protein of purifying will have effectiveness and function more widely at existing and potential food and medicine field.
The byproduct that is produced when the present invention prepares alpha corn protein (as glutelin, grease and starch) also can further separate, and prepares natural vegetable protein additive and food grade colorant.
Description of drawings
Fig. 1 is concrete process route view.
The specific embodiment
Below in conjunction with embodiment the present invention is further elaborated.
Embodiment 1
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 5 times of volumes then, degreasing 2 times; Degreasing zein particle adds 80% ethanol of 6 times of volumes, room temperature adverse current lixiviate 24 hours, Separation of Solid and Liquid; Filtrate adds the NaCl aqueous solution of 2 times of volumes 0.25%, and gained precipitation water washes 3 times repeatedly, eliminates NaCl wherein.Carry out vacuum drying then and make yellow corn protein 28g.
Embodiment 2
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 5 times of volumes then, degreasing 2 times; Degreasing zein particle adds 80% ethanol of 6 times of volumes, room temperature adverse current lixiviate 24 hours, Separation of Solid and Liquid; The pH of filtrate is adjusted to 5.5, adds 10% active carbon powder, under 30 ℃ of conditions, stir 5h; Destainer adopts high speed centrifugation to carry out Separation of Solid and Liquid; Filtrate decompression is concentrated into 1/2 volume, reclaims ethanol, carry out vacuum drying then and make white zein 25g.
Embodiment 3
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 7 times of volumes then, degreasing 2 times; Degreasing zein particle adds 90% ethanol of 10 times of volumes, room temperature adverse current lixiviate 12 hours, Separation of Solid and Liquid; The pH of filtrate is adjusted to 6.5, adds 6% active carbon powder, under 60 ℃ of conditions, stir 2h; Destainer adopts vacuum filtration to carry out Separation of Solid and Liquid; Filtrate adds the NaCl aqueous solution of 3 times of volumes 1%, and gained precipitation water washes 3 times repeatedly, eliminates NaCl wherein; Carry out vacuum drying then and make white alpha corn protein 27g.
Embodiment 4
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 5 times of volumes then, degreasing 2 times; Degreasing zein particle adds 90% ethanol of 6 times of volumes, room temperature adverse current lixiviate 24 hours, Separation of Solid and Liquid; The pH of filtrate is adjusted to 6.5, adds 10% active carbon powder, under 30 ℃ of conditions, stir 5h; Destainer adopts high speed centrifugation to carry out Separation of Solid and Liquid; Filtrate as protective medium, makes high-purity, high-quality alpha corn protein 27.5g through closed circuit spray-drying with nitrogen, reclaims ethanol simultaneously.
Embodiment 5
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 10 times of volumes then, degreasing 2 times; Degreasing zein particle adds 90% ethanol of 4 times of volumes, room temperature adverse current lixiviate 12 hours, Separation of Solid and Liquid; The pH of filtrate is adjusted to 6.8, adds 1% Emathlite, under 60 ℃ of conditions, stir 4h; Destainer adopts press filtration to carry out Separation of Solid and Liquid; Filtrate decompression is concentrated into 1/2 volume, reclaims ethanol, carry out vacuum drying then and make alpha corn protein 27.1g.
Embodiment 6
100g corn protein powder (moisture 5%) is granulated, and adds the absolute ethyl alcohol of 10 times of volumes then, degreasing 2 times; Degreasing zein particle adds 90% ethanol of 4 times of volumes, room temperature adverse current lixiviate 12 hours, Separation of Solid and Liquid; The pH of filtrate is adjusted to 6.5, adds 2.5% Emathlite, under 60 ℃ of conditions, stir 4h; Destainer adopts press filtration to carry out Separation of Solid and Liquid; Filtrate as protective medium, makes high-purity, high-quality alpha corn protein 27.5g through closed circuit spray-drying with nitrogen.Reclaim ethanol simultaneously.
Claims (4)
1. the preparation method of a high purity alpha corn protein is characterized in that:
(1) corn protein powder is granulated, and adds the absolute ethyl alcohol of 5～10 times of volumes then, degreasing 2 times; Adopt vacuum filtration or the centrifugal Separation of Solid and Liquid of carrying out;
(2) step 1 degreasing zein particle being added 4～10 times of volumes, concentration is 80～95% ethanol, room temperature adverse current lixiviate 4～24 hours, Separation of Solid and Liquid;
(3) filtrate of step 2 is regulated pH in 5.5～6.8 scopes, add active carbon or Emathlite then, addition and destainer mass ratio are 1～10%, stir 1～5h under 25～70 ℃ of conditions; The gained mixed liquor adopts vacuum filtration, high speed centrifugation or press filtration to carry out Separation of Solid and Liquid; Its filter residue is the processing of can regenerating of active carbon or Emathlite, reuses;
(4) filtrate of step 3 is handled the final vacuum drying, make alpha corn protein.
2. the preparation method of a kind of high purity alpha corn protein according to claim 1, it is characterized in that the filtrate of step 3 is added the NaGl aqueous solution of 2～4 times of volumes 0.25～1%, gained precipitation water cyclic washing 3 times, eliminate NaCl wherein, carry out vacuum drying then and make alpha corn protein.
3, the preparation method of a kind of high purity alpha corn protein according to claim 1 is characterized in that the filtrate of step 3 is evaporated to 1/2 volume, reclaims ethanol, carries out vacuum drying then and makes alpha corn protein;
4, the preparation method of a kind of high purity alpha corn protein according to claim 1, it is characterized in that filtrate with step 3 with nitrogen as protective medium, make high-purity, high-quality alpha corn protein through closed circuit spray-drying, reclaim ethanol simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100860107A CN100571530C (en) | 2006-07-19 | 2006-07-19 | A kind of preparation method of high purity alpha corn protein |
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| CNB2006100860107A CN100571530C (en) | 2006-07-19 | 2006-07-19 | A kind of preparation method of high purity alpha corn protein |
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| CN1899076A CN1899076A (en) | 2007-01-24 |
| CN100571530C true CN100571530C (en) | 2009-12-23 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703146B (en) * | 2009-10-30 | 2011-11-02 | 华南理工大学 | Method for purifying food-grade corn protein powder |
| CN103570815B (en) * | 2013-09-17 | 2015-01-21 | 中国科学院长春应用化学研究所 | Extraction method of alpha-zein component |
| CN104642713B (en) * | 2015-01-26 | 2018-02-27 | 江苏大学 | Zein continuous vacuum drum drying equipment and method |
| CN107849099B (en) | 2015-03-24 | 2022-03-22 | 嘉吉公司 | Corn protein isolate and method for producing same |
| MX2018011370A (en) | 2016-03-24 | 2019-02-13 | Cargill Inc | Corn protein product having decreased free sulfite levels & method for manufacturing same. |
| MX2019003316A (en) | 2016-09-23 | 2019-08-21 | Cargill Inc | Corn protein retention during extraction. |
| US11980217B2 (en) | 2017-08-02 | 2024-05-14 | Cargill, Incorporated | Extruded corn protein material |
| US11667670B2 (en) | 2017-09-21 | 2023-06-06 | Cargill, Incorporated | Corn protein retention during extraction |
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Non-Patent Citations (2)
| Title |
|---|
| 玉米醇溶蛋白的制备. 刘志国等.武汉食品工业学院学报，第1997年第3期. 1997 |
| 玉米醇溶蛋白的制备. 刘志国等.武汉食品工业学院学报，第3期. 1997 * |
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