CN104804106A - Purification method of high-purity polysaccharides of cistanche deserticola - Google Patents
Purification method of high-purity polysaccharides of cistanche deserticola Download PDFInfo
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- CN104804106A CN104804106A CN201510177055.4A CN201510177055A CN104804106A CN 104804106 A CN104804106 A CN 104804106A CN 201510177055 A CN201510177055 A CN 201510177055A CN 104804106 A CN104804106 A CN 104804106A
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- based diet
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Abstract
The invention discloses a purification method of high-purity polysaccharides of cistanche deserticola. The method comprises is characterizing by comprising processes as follows: firstly, cistanche deserticola coarse powder is added to hot water and subjected to reflux for 2 h at the temperature of 60 DEG C-90 DEG C, an extracting solution is obtained after filtration washing and then is left to stand, cooled and filtered, filtrate is subjected to decoloration and purification through macroporous resin, an effluent liquid passes by ion resin, a polysaccharide solution is obtained, and finally, the high-purity polysaccharides of the cistanche deserticola is obtained after spraying drying. According to the purification method, the procedure is clear, the method is simple, the cost is low, no environmental pollution is caused, a product obtained through purification has good color, high yield and purity and is suitable for industrial mass production, characteristics of different types of pigments and impurities of the cistanche deserticola are sufficiently applied, the high-purity polysaccharide component is obtained, and a basis is provided for wider and effective application of polysaccharides of the cistanche deserticola.
Description
Technical field
The present invention relates to a kind of purification process of high purity wheat-based diet, belong to chemical purification technical field.
Background technology
The good reputation that Herba Cistanches have " desert ginseng ", has high pharmaceutical use, is the rare traditional Chinese medicine of Chinese tradition.Herba Cistanches taste is sweet, warm in nature, the effect such as have kidney invigorating and YANG supporting, benefiting essence and marrow, enriching blood and moistening for dryness, happy look are prolonged life.Herba Cistanches medicine-food two-purpose, long-term eating can increase muscle power, stamina intensifying and resisting fatigue, can strengthen again sexuality and the fertility of the mankind and animal simultaneously.Wheat-based diet is one of main component of Herba Cistanches pharmacological action, and modern age, pharmaceutical research showed, wheat-based diet can improve body immunity, promotes DNA synthesis, physical strength reinforcing, improves intelligence, have significant anti-aging effects.But the purity of polysaccharide directly affects the degree of its pharmacological action, the high wheat-based diet of therefore separation and purification purity has important social effect for the Application and Development of Herba Cistanches.
There are reports in the current research of the extraction purification for wheat-based diet.The patent No. is that the patent of invention " extraction process of Herba Cistanches medicinal material effective ingredient " of CN200810025392.1 discloses a kind of Herba Cistanches hot water extraction and obtains the method for wheat-based diet through concentrated alcohol precipitation, this method technique is simple, but the polysaccharide color extracted is not good, loss is large, the impurity components such as purity is low, some albumen are difficult to effectively be removed, and the patent of invention of patent No. CN201010213839.5 " a kind of separating and purifying method of wheat-based diet " discloses through sherwood oil and alcohol immersion process degreasing, cross alcohol precipitation after macroporous adsorbent resin, again through acetone, ether and Ethanol Treatment obtain the method for wheat-based diet, this method is compared with traditional method, although add many degreasings and organic solvent treating processes, some other component substances can be removed preferably, but polysaccharide yield is low, the polysaccharide color obtained and purity are still difficult to meet high-quality requirement, and the solvent species that this technique uses is many, also the requirement of suitability for industrialized production is unfavorable for.
Summary of the invention
In order to overcome prior art problem, the object of the present invention is to provide a kind of simple and fast, being suitable for the purification process of the high purity wheat-based diet of suitability for industrialized production.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of purification process of high purity wheat-based diet, it is characterized in that, process is: first join in hot water by Herba Cistanches meal, reflux 2h under the temperature condition of 60-90 DEG C, obtains extracting solution, extracting solution is left standstill cooled and filtered after filtration washing, then filtrate to be decoloured removal of impurities through macroporous resin, effluent liquid by ion exchange resin, obtains polysaccharide soln, finally by obtaining high purity wheat-based diet after spraying dry again.
Further, described reflux temperature is preferably 75 DEG C.
And the model of described macroporous resin is any one in D101, ADS-7, HPD-600, X-5, AB-8.Be preferably D101.
Described ion exchange resin is resin cation (R.C.) or resin anion(R.A).And the model of described ion exchange resin be 732, JK008,717, any one in D900.Be preferably 732.
Beneficial effect of the present invention is: purification process clear process of the present invention, method are simple, low and the non-environmental-pollution of cost, the product colour that purifying obtains is good, yield and purity high, be suitable for industrialized production, and fully apply the characteristic of the dissimilar pigment of Herba Cistanches and impurity, obtain highly purified polysaccharide component, for wheat-based diet can obtain more extensively and effectively applying providing foundation.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment.
embodiment 1:
First Herba Cistanches meal 1kg is joined in 6L purified water, reflux 2 hours under the temperature condition of 75 DEG C, extracting solution 6.3 L is obtained after filtration washing, filter residue again adds above-mentioned equivalent purified water and continues backflow 2 hours, filters, and merges extracted twice liquid, be roughly equal to 12.5 L, extracting solution is left standstill cooled and filtered, then by filtrate through D101 macroporous resin decolouring removal of impurities, directly collect effluent liquid after washing.Effluent liquid is added 732 type ion exchange resin posts, collect effluent liquid, spraying dry obtains the material 118.5g of white powder, test (alpha-naphthol test), anthrone-sulfuric acid process test by Molish, Fehling test discriminating is detected as wheat-based diet, and reach 98.23% by phenol-sulfuric acid and colorimetric method mensuration content.
embodiment 2:
First Herba Cistanches meal 1kg is joined in 6L purified water, reflux 2 hours under the temperature condition of 90 DEG C, extracting solution 6.1 L is obtained after filtration washing, filter residue again adds above-mentioned equivalent purified water and continues backflow 2 hours, filters, and merges extracted twice liquid, be roughly equal to 12.9 L, extracting solution is left standstill cooled and filtered, then by filtrate through D101 macroporous resin decolouring removal of impurities, directly collect effluent liquid after washing.Effluent liquid is added D900 type ion exchange resin post, collect effluent liquid, spraying dry obtains the material 123.3g of white powder, be wheat-based diet after testing, and content reaches 95.28%.
embodiment 3:
First Herba Cistanches meal 1kg is joined in 6L purified water, reflux 2 hours under the temperature condition of 60 DEG C, extracting solution 5.8 L is obtained after filtration washing, filter residue again adds above-mentioned equivalent purified water and continues backflow 2 hours, filters, and merges extracted twice liquid, be roughly equal to 11.4 L, extracting solution is left standstill cooled and filtered, then by filtrate through AB-8 macroporous resin decolouring removal of impurities, directly collect effluent liquid after washing.Effluent liquid is added JK008 type ion exchange resin post, collect effluent liquid, spraying dry obtains the material 108.3g of white powder, be wheat-based diet after testing, and content reaches 96.58%.
The present invention is illustrated according to above-described embodiment and should be appreciated that above-described embodiment does not limit the present invention in any form, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (7)
1. the purification process of a high purity wheat-based diet, it is characterized in that, process is: first join in hot water by Herba Cistanches meal, reflux 2h under the temperature condition of 60-90 DEG C, obtains extracting solution, extracting solution is left standstill cooled and filtered after filtration washing, then filtrate to be decoloured removal of impurities through macroporous resin, effluent liquid by ion exchange resin, obtains polysaccharide soln, finally by obtaining high purity wheat-based diet after spraying dry again.
2. the purification process of a kind of high purity wheat-based diet according to claim 1, is characterized in that, described reflux temperature is 75 DEG C.
3. the purification process of a kind of high purity wheat-based diet according to claim 1, is characterized in that, the model of described macroporous resin is any one in D101, ADS-7, HPD-600, X-5, AB-8.
4. the purification process of a kind of high purity wheat-based diet according to claim 1 or 3, is characterized in that, the model of described macroporous resin is D101.
5. the purification process of a kind of high purity wheat-based diet according to claim 1, is characterized in that, described ion exchange resin is resin cation (R.C.) or resin anion(R.A).
6. the purification process of a kind of high purity wheat-based diet according to claim 1 or 5, is characterized in that, the model of described ion exchange resin is 732, JK008,717, any one in D900.
7. the purification process of a kind of high purity wheat-based diet according to claim 6, is characterized in that, the model of described ion exchange resin is 732.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115227741A (en) * | 2022-06-09 | 2022-10-25 | 新疆汇峰农业科技有限公司 | Cistanche deserticola extract purification method and equipment |
| CN116531802A (en) * | 2023-05-17 | 2023-08-04 | 甘肃省农业科学院农产品贮藏加工研究所 | Cistanche deserticola polysaccharide obtaining and purifying equipment and method |
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| CN101265161A (en) * | 2008-04-25 | 2008-09-17 | 江苏省农业科学院 | Technique for extracting cynamorlum herba cistanches medicinal materials effective component |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115227741A (en) * | 2022-06-09 | 2022-10-25 | 新疆汇峰农业科技有限公司 | Cistanche deserticola extract purification method and equipment |
| CN116531802A (en) * | 2023-05-17 | 2023-08-04 | 甘肃省农业科学院农产品贮藏加工研究所 | Cistanche deserticola polysaccharide obtaining and purifying equipment and method |
| CN116531802B (en) * | 2023-05-17 | 2024-04-05 | 甘肃省农业科学院农产品贮藏加工研究所 | Cistanche deserticola polysaccharide obtaining and purifying equipment and method |
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| CN104804106B (en) | 2017-07-18 |
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Address after: 212415 Jurong city of Jiangsu province Baohua Town Development Zone No. 10 Patentee after: Jiangsu Tiansheng Pharmaceutical Co., Ltd. Address before: 212415 Zhenjiang city of Jiangsu province Jurong Baohua Development Zone No. 10 Patentee before: Jiangsu Tiansheng Pharmaceutical Co., Ltd. |