JPH0446109A - Cosmetic - Google Patents
CosmeticInfo
- Publication number
- JPH0446109A JPH0446109A JP15148890A JP15148890A JPH0446109A JP H0446109 A JPH0446109 A JP H0446109A JP 15148890 A JP15148890 A JP 15148890A JP 15148890 A JP15148890 A JP 15148890A JP H0446109 A JPH0446109 A JP H0446109A
- Authority
- JP
- Japan
- Prior art keywords
- cosmetics
- vitronectin
- present
- cosmetic
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002537 cosmetic Substances 0.000 title claims abstract description 28
- 108010031318 Vitronectin Proteins 0.000 claims abstract description 24
- 102100035140 Vitronectin Human genes 0.000 claims abstract description 24
- 238000002360 preparation method Methods 0.000 abstract description 6
- 241001465754 Metazoa Species 0.000 abstract description 4
- 230000003213 activating effect Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 210000001124 body fluid Anatomy 0.000 abstract description 2
- 239000010839 body fluid Substances 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000003906 humectant Substances 0.000 abstract description 2
- 239000003921 oil Substances 0.000 abstract description 2
- 239000003755 preservative agent Substances 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 239000004094 surface-active agent Substances 0.000 abstract description 2
- 230000002335 preservative effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 108090000288 Glycoproteins Proteins 0.000 description 5
- 102000003886 Glycoproteins Human genes 0.000 description 5
- 239000006210 lotion Substances 0.000 description 5
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 4
- 230000020411 cell activation Effects 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 4
- 102100037362 Fibronectin Human genes 0.000 description 3
- 108010067306 Fibronectins Proteins 0.000 description 3
- -1 Polyoxyethylene cetyl ether Polymers 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 3
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 3
- 229960002216 methylparaben Drugs 0.000 description 3
- 210000000434 stratum corneum Anatomy 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 description 2
- QFOHBWFCKVYLES-UHFFFAOYSA-N Butylparaben Chemical compound CCCCOC(=O)C1=CC=C(O)C=C1 QFOHBWFCKVYLES-UHFFFAOYSA-N 0.000 description 2
- 239000004166 Lanolin Substances 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 229940075507 glyceryl monostearate Drugs 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 229940039717 lanolin Drugs 0.000 description 2
- 235000019388 lanolin Nutrition 0.000 description 2
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 2
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 2
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 229940032094 squalane Drugs 0.000 description 2
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102100031673 Corneodesmosin Human genes 0.000 description 1
- 101710139375 Corneodesmosin Proteins 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 229940067596 butylparaben Drugs 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 229940074928 isopropyl myristate Drugs 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 229940012831 stearyl alcohol Drugs 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Cosmetics (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は化粧料に関する。さらに詳しくは、皮膚に塗布
した場合、細胞賦活効果を有するビトロネクチンを配合
してなる化粧料に関する。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to cosmetics. More specifically, the present invention relates to a cosmetic containing vitronectin, which has a cell activating effect when applied to the skin.
（従来の技術及び発明が解決しようとする課題）化粧料
には細胞賦活剤としてホルモン類などの薬剤が配合され
ている。本発明者は、化粧料に細胞賦活性を付与し、よ
り機能的な化粧料を開発すべく、細胞賦活性を有する物
質を種々探求した結果、糖蛋白質の一種であるビトロネ
クチンが細胞賦活剤効果に優れており、また調製時や滅
菌時の加熱にも耐えることに着目し、本発明を完成した
。(Prior Art and Problems to be Solved by the Invention) Cosmetics contain drugs such as hormones as cell activators. In order to impart cell activation to cosmetics and develop more functional cosmetics, the present inventor investigated various substances with cell activation, and as a result, found that vitronectin, a type of glycoprotein, has a cell activation effect. The present invention was completed by focusing on the fact that it has excellent properties and can withstand heating during preparation and sterilization.
従来より、ビトロネクチンは動物の血液中に存在する糖
蛋白質で、細胞接着、血液疑固、免疫補体、ガンの転移
などに重要な役割を果たしていることは良く知られてい
た。そのため、その応用は、医療方面が主として行われ
ていたが、化、註料への配合についての報告はまたなさ
れていない。It has long been well known that vitronectin is a glycoprotein present in the blood of animals and plays an important role in cell adhesion, blood pseudocoagulation, immune complement, cancer metastasis, etc. Therefore, its application has been mainly carried out in the medical field, but there have been no reports on its formulation or incorporation into dietary supplements.
（課題を解決するための手段）
本発明は、ビトロネクチンを配合することを特徴とする
化粧料である。(Means for Solving the Problems) The present invention is a cosmetic characterized by containing vitronectin.
本発明で用いられるビトロネクチンは動物体液及び組織
中に存在している分子量６０，０００〜ｓｏ、ｏｏ。The vitronectin used in the present invention is present in animal body fluids and tissues and has a molecular weight of 60,000 to so, oo.
の糖蛋白質で、血清中細胞進展因子、Ｓ蛋白質、エビボ
リンなどと呼ばれるものである。These glycoproteins are called serum cell growth factor, S protein, evivolin, etc.
本発明におけるビトロネクチンは、ヒト又は動物の血液
より採取、精製したものを使用することが好ましい。抽
出法は、生体中、例えば血漿中に存在するビトロネクチ
ンを、尿素の存在下でグリコサミノグリカン固定化担体
に特異的に結合させて精製する方法（特開昭６３−１２
５９９０号公報）が好ましい。採取、精製したビトロネ
クチンは、オートクレーブ処理を行い混在する可能性の
ある肝炎つィルス等を滅菌してから使用する。The vitronectin used in the present invention is preferably collected and purified from human or animal blood. The extraction method involves purifying vitronectin, which exists in living organisms, such as plasma, by specifically binding it to a glycosaminoglycan-immobilized carrier in the presence of urea (Japanese Patent Laid-Open No. 63-12
No. 5990) is preferred. The collected and purified vitronectin is autoclaved to sterilize hepatitis viruses and the like that may be present before use.
本発明におけるビトロネクチンの化粧料への配合量は、
化粧料全量の０．００００１〜１．０重量％であり、さ
らに好ましくは、０．００１〜０．１重量％である。ま
た、必要に応じて、油分、界面活性剤、保湿剤、防腐剤
等の一般に化粧料に用いられる原料を添加することもで
きる。In the present invention, the amount of vitronectin added to the cosmetic is as follows:
The content is 0.00001 to 1.0% by weight, more preferably 0.001 to 0.1% by weight, based on the total amount of cosmetics. Furthermore, raw materials commonly used in cosmetics, such as oil, surfactants, humectants, and preservatives, can also be added, if necessary.
本発明の化粧料は、皮膚細胞に対し賦活作用があり、保
湿効果を高め、肌にはり、なめらかさ、しっとりさをも
たらす。また、化粧料に糖蛋白質の一種であるフィブロ
ネクチンを配合することが知られているが（特開昭５９
−７６００７号公報）、フィブロネクチンは熱に対して
不安定であるため、滅菌することが困難であり、また、
クリーム等の乳化タイプの化粧料に配合する場合、高温
で添加できないという欠点がある。しかし、本発明に使
用するビトロネクチンは、耐熱性を有するため、オート
クレーブによる滅菌処理が可能であり、原料に由来する
肝炎ウィルス等の生菌が混入する危険性がなく、また調
製時に高温で他の化粧料と配合することができる。The cosmetic of the present invention has a activating effect on skin cells, enhances the moisturizing effect, and provides firmness, smoothness, and moisture to the skin. It is also known that fibronectin, a type of glycoprotein, is added to cosmetics (Japanese Patent Application Laid-Open No.
Since fibronectin is unstable to heat, it is difficult to sterilize it, and
When incorporated into emulsified cosmetics such as creams, it has the disadvantage that it cannot be added at high temperatures. However, since the vitronectin used in the present invention is heat resistant, it can be sterilized by autoclaving, and there is no risk of contamination with live bacteria such as hepatitis viruses derived from raw materials. Can be combined with cosmetics.
次に実施例によって本発明を説明する。なお、本発明は
、これによって限定されるものではなり）。Next, the present invention will be explained by examples. Note that the present invention is not limited to this.)
実施例１． 化粧水の調製例
［成分］ 重量％ビトロ
ネクチン ０．０５グリセリン
２・５１．３−ブチレングリコ
ール ３．５メチルパラベン
０．１ポリオキシエチレン
ソルビタンモノラウレート ０．５香料
０・０５精製水
９３．３〔調製法〕
精製水に、ビトロネクチン、グリセリン及び１．３−ブ
チレングリコールを溶解する。別（二番粉、ポリオキシ
エチレンソルビタンモノラウレート及びメチルパラベン
を溶解し、これを前述の溶液番こ加えて均一に溶解し、
濾過して調製する。Example 1. Preparation example of lotion [Ingredients] Weight % vitronectin 0.05 glycerin
2.51.3-Butylene glycol 3.5 Methylparaben
0.1 Polyoxyethylene sorbitan monolaurate 0.5 Fragrance
0.05 Purified water
93.3 [Preparation method] Dissolve vitronectin, glycerin and 1,3-butylene glycol in purified water. Separately (dissolve the second powder, polyoxyethylene sorbitan monolaurate and methyl paraben, add this to the solution number mentioned above and dissolve it uniformly,
Prepare by filtering.
この実施例１の化粧水を用いて、長期連用時（こおける
インピーダンスメーターによる各層水分含有量測定、及
び官能テストを行った。対照として、ビトロネクチン未
配合のものを用いた。Using this lotion of Example 1, water content was measured in each layer using an impedance meter and a sensory test was conducted during long-term use.As a control, a lotion without vitronectin was used.
（長期連用試験）
長期連用試験は、被験者の前腕局側を被験部位とし、毎
日朝と夜に化粧水を適量塗布し、塗布前、塗布開始２週
間後、同４週間後、同６週間後、同８週間後、塗布終了
後１週間後、同２週間後に行った。(Long-term continuous use test) In the long-term continuous use test, the subject's forearm was used as the test site, and an appropriate amount of lotion was applied every day in the morning and evening. , 8 weeks later, 1 week after the completion of the application, and 2 weeks after the completion of the application.
その結果を第１図のグラフに示す。このグラフより明ら
かなように、ビトロネクチン配合のものは対照のビトロ
ネクチン未配合のものよりも角質層の水分量が増えてき
ており、角質層の水分保持機能が向上していることがわ
かる。The results are shown in the graph of FIG. As is clear from this graph, the water content in the stratum corneum of the product containing vitronectin increased compared to the control product containing no vitronectin, indicating that the water retention function of the stratum corneum was improved.
（官能テスト）
長期連用試験中と、塗布終了後の肌の感触と使用感につ
いてパネルテスト（パネラ−１５人）を行った。その結
果は次の通りである。(Sensory test) A panel test (15 panelists) was conducted regarding the skin feel and feeling of use during the long-term continuous use test and after application. The results are as follows.
ビトロネクチン配合の化粧水を使用することにより、肌
のはり、表面のなめらかさ、しつとりさを改善し、使用
感を向上することができた。By using a lotion containing vitronectin, it was possible to improve the firmness of the skin, the smoothness of the surface, and the moisture, and improve the feeling of use.
実施例２． クリームの調製
〔成分］ 重量％ステ
アリルアルコール ５．０スクアラン
３．０ラノリン
１，０イソプロピルミリステート
１．０モノステアリン酸グリセリン
１．５ポリオキシエチレンセチルエーテル １．００
．１
０．１
メチルパラベン
プチルパラベン
グリセリン ２．５ヒドロネク
チン ０．０１香料
０．０５精製水
８４．７９［調製法］
ステアリルアルコール、スクアラン、ラノリン、イソプ
ロピルミリステート、モノステアリン酸グリセリン、ポ
リオキシエチレンセチルエーテル及びブチルパラベンを
秤り込み８０〜８５℃に加熱し、溶解する。別にメチル
パラベン、グリセリン、ヒドロネクチン及び精製水を秤
り込み８０〜８５℃で加熱溶解し、前述の溶液に徐々に
加え乳化する。撹拌しながら、冷却し、６０℃で香料を
加え、さらに３０℃まで冷却して精製する。Example 2. Preparation of cream [Ingredients] Weight % stearyl alcohol 5.0 squalane
3.0 lanolin
1,0 isopropyl myristate
1.0 Glyceryl monostearate
1.5 Polyoxyethylene cetyl ether 1.00
．． 1 0.1 Methylparabenbutylparabenglycerin 2.5 Hydronectin 0.01 Fragrance
0.05 purified water
84.79 [Preparation method] Stearyl alcohol, squalane, lanolin, isopropyl myristate, glyceryl monostearate, polyoxyethylene cetyl ether, and butyl paraben are weighed and heated to 80 to 85°C to dissolve. Separately, methylparaben, glycerin, hydronectin, and purified water are weighed out, heated and dissolved at 80 to 85°C, and gradually added to the above solution to emulsify. While stirring, cool, add perfume at 60°C, and further cool to 30°C for purification.
（本発明の効果）
本発明におけるビトロネクチンを配合した化粧料は、細
胞賦活効果が優れており、そのため皮膚に塗布した場合
に皮膚各層の含有水分量を増加させることができ、肌の
はり、なめらかさ、しっとりさ等の感触を向上させるこ
とができる。また、ビトロネクチンは、熱に対して安定
性が良いため、化粧品調製時に温度を上げて混合を良く
することができ、更に加熱滅菌処理が可能であるので、
本発明の化粧品は、フィブロネクチンなどの他の糖蛋白
質を配合した従来の化粧品と比べて質的に優れている。(Effects of the present invention) The cosmetic containing vitronectin according to the present invention has an excellent cell activation effect, and therefore, when applied to the skin, it can increase the moisture content in each layer of the skin, making the skin firm and smooth. It is possible to improve the texture such as softness and moistness. In addition, vitronectin has good stability against heat, so it is possible to increase the temperature when preparing cosmetics to improve mixing, and it can also be heat sterilized.
The cosmetics of the present invention are qualitatively superior to conventional cosmetics containing other glycoproteins such as fibronectin.
第１図は、本発明のビトロネクチン配合の化粧料とビト
ロネクチン未配合の化粧料との角質層水分量を比較した
グラフである。FIG. 1 is a graph comparing the moisture content of the stratum corneum between a cosmetic containing vitronectin of the present invention and a cosmetic containing no vitronectin.
Claims (1)
。1. A cosmetic characterized by containing vitronectin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15148890A JPH0446109A (en) | 1990-06-12 | 1990-06-12 | Cosmetic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15148890A JPH0446109A (en) | 1990-06-12 | 1990-06-12 | Cosmetic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0446109A true JPH0446109A (en) | 1992-02-17 |
Family
ID=15519597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15148890A Pending JPH0446109A (en) | 1990-06-12 | 1990-06-12 | Cosmetic |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0446109A (en) |
-
1990
- 1990-06-12 JP JP15148890A patent/JPH0446109A/en active Pending
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