JPH05262635A - Dermatic external preparation - Google Patents

Abstract

PURPOSE:To provide a dermatic external preparation having high skin chapping improving effects, further improved in the cell growth promoting activity of cycosaponin b1 and cycosaponin b2. CONSTITUTION:The objective dermatic external preparation containing (A) cycosaponin b1 and/or cycosaponin b2 and (B) at least one kind selected from glycyrrhizic acid, its salts and derivatives, pantothenic acid, its salts and derivatives, spleen extracts, allantoin, hinokitiol, placenta extracts, hamamelis, vitamin C, its salts and derivatives, vitamin E, its salts and derivatives, collagen, carrot extracts, and L-arginine-L-aspartic acid.

Description

Detailed Description of the Invention

[0001]

The present invention relates to a skin external preparation,
More specifically, it relates to a skin external preparation having a high skin cell growth effect. The external preparation for skin is preferably applied to cosmetics such as lotions, creams, emulsions, packs, scalp cosmetics, and pharmaceuticals such as wound treatment and anti-inflammatory ointment.

[0002]

BACKGROUND OF THE INVENTION Psychosaponin extracted from plants such as Saiko has effects such as wound healing of skin, prevention and improvement of skin roughness, and aging prevention to prevent sagging and loss of skin. It is compounded.

[0003] The extracted saikosaponin is saikosaponin a, saikosaponin b ₁ , saikosaponin b.
_2. A technique in which the above effects are enhanced by separating each of Psychosaponin c and Psychosaponin d and using each component independently has also been proposed (JP-A-61-2216).

[0004] However, there is a demand for an external preparation for the skin which further enhances the above effects.

[0005]

DISCLOSURE OF THE INVENTION The present invention relates to a skin external preparation which is further improved in effects such as wound healing of skin, prevention and improvement of skin roughness and aging prevention to prevent sagging and loss of skin. The purpose is to provide.

[0006]

That is, the invention according to claim 1 relates to saikosaponin b ₁ and / or saikosaponin b ₂ and glycyrrhizic acid, a salt of glycyrrhizic acid and a glycyrrhizic acid derivative, a salt of pantothenic acid, a salt of pantothenic acid, and Pantothenic acid derivative, spleen extract, allantoin, hinokitiol, placenta extract, vitamin C, vitamin C salt and vitamin C derivative, vitamin E, vitamin E salt and vitamin E derivative, hamamelis extract, collagen, carrot extract And one or more selected from L-arginine-L-aspartic acid, and the invention according to claim 2 provides the external preparation for skin according to claim 1. in the invention, selected from saikosaponins b ₁ and / or saikosaponins b ₂ A seed or two or 0.0001
The present invention provides a skin external preparation characterized by containing 20 to 20% by weight.

The structure of the present invention will be described in detail below.

The external preparation for skin of the present invention contains at least _{one of} saikosaponin b ₁ and saikosaponin b ₂ . The compounding amount is 0.000 in the total amount of the external preparation for skin.
1 to 20% by weight is preferable, and 0.001 to 10% by weight is more preferable. When it is 0.001% by weight or more, the effect of improving skin roughness is further enhanced. On the other hand, if it is 10% by weight or less, the sticky feeling is lost and the stimulative effect is lost.

Glycyrrhizic acid used in the present invention is known to be effective for acute and chronic dermatitis in the dermatological field closely related to cosmetics, with many clinical reports as an external preparation.

The salts of glycyrrhizic acid include K, Na,
Examples of metal salts and derivatives of Ca and the like include monoammonium glycyrrhizinate, pyridoxine glycyrrhizinate, stearyl glycyrrhizinate, and the like.

Pantothenic acid, its salts and its derivatives used in the present invention are water-soluble vitamins involved in the biosynthesis of coenzyme A.

The salts of pantothenic acid include K, Na and Ca.
Examples of metal salts and derivatives thereof include pantothenyl ether and the like.

The spleen extract used in the present invention may be, for example, any extract extracted from spleens of mammals other than humans.

Allantoin used in the present invention is an oxide of uric acid, and can be obtained from, for example, feces of a stool.

The hinoki thiol used in the present invention is an extract obtained from hinoki.

The placenta extract used in the present invention is an extract obtained from the placenta of mammals other than humans.

As vitamin C, its salts and derivatives used in the present invention, ascorbic acid, ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, L-ascorbic acid-2-sodium sulfate, magnesium ascorbyl phosphate ( Phosphoric acid-L
-Ascorbyl magnesium), and the like.

Examples of vitamin E, salts thereof and derivatives thereof used in the present invention include dl-α-tocopherol and dl-α-tocopherol acetate.

Hamamelis extract Ha used in the present invention
mamelis Verginiona Linnac
It is an extract obtained from us.

The collagen used in the present invention is obtained by subjecting dermal tissues of mammals other than humans to pepsin treatment and then extracting and purifying.

The carrot extract used in the present invention is, for example, Panax ginseng.
C. A. Examples thereof include those obtained by low-temperature extraction of the components contained in Meyet with ethanol.

L-arginine-L- used in the present invention
Aspartic acid is an amide bond of L-arginine and L-aspartic acid.

In the present invention, the glycyrrhizic acid, salts thereof and derivatives thereof, pantothenic acid, salts thereof and derivatives thereof, spleen extract, allantoin, hinokitiol, placenta extract, vitamin C, salts thereof and derivatives thereof, vitamins E, salts and derivatives thereof, hamamelis extract, collagen, carrot extract, and L-arginine-L-aspartic acid are used alone or in combination of two or more. 0.01 to 10.0% by weight is preferable, and 0.1 to 10.0% by weight is preferable.
5.0% by weight is more preferable. When it is 0.1% by weight or more, the skin cell proliferation effect is further improved, and the feeling of use such as moisturizing and moisturizing feeling is further improved. When the amount is 5.0% by weight or less, the feeling of use is further improved such that the stickiness is eliminated.

As described above, glycyrrhizic acid, its salt and its derivative, pantothenic acid, its salt and its derivative, spleen extract, allantoin, hinokitio-
, Placenta extract, vitamin C, salts and derivatives thereof, vitamin E, salts and derivatives thereof, hamamelis extract, collagen, carrot extract, L-arginine-L-aspartic acid, saikosaponin b ₁ or psycho. It has the effect of synergistically improving the skin cell growth effect of saponin b ₂ . That is, by blending both together, the skin cell proliferation effect of Psychosaponins b ₁ and b ₂ , that is, the wound healing effect and the skin roughening improving effect are remarkably improved.

The external preparation for skin of the present invention contains, in addition to the above-mentioned essential components, components used in cosmetics, pharmaceuticals and the like, such as an aqueous component, a powder component, an oil component and a surface active agent, as long as the effects of the present invention are not impaired. Agents, moisturizers, thickeners, preservatives, antioxidants, fragrances, coloring agents, drug components and the like can be added.

Among these, in particular, as drug ingredients, ethinyl estradiol, diethylstilbestrol, hydrocortisone, prednisolone, hydrocortisone acetate, pregnenolone acetate, loquat extract,
Assembly extract, aloe extract, glycyrrhetinic acid, L
-Sodium glutamate, ethyl paraaminobenzoate, serine, glycine, arginine, lysine, aspartic acid, cysteine, diphenhydramine hydrochloride, royal jelly extract, γ-oryzanol, nicotinic acid amide, benzyl nicotinate, apple extract, lemon extract. , Vitamin K, Vitamin H, rutin, horse chestnut extract, horse mackerel extract, birch extract, yarrow extract, thyme extract, lily extract, sycamore extract, burdock extract, loofah extract, epidihydrocholesterol, protease, papain, duckweed extract, elastin. , Keratin, cystine, dl-pyrrolidonecarboxylic acid and salts thereof, chondroitin sulfate, hyaluronic acid, rice extract, Cordyceps sinensis extract, Yukinoshita extract, iris extract and the like. That.

Further, the dosage form of the external preparation for skin is arbitrary, and for example, a solubilizing system such as lotion, an emulsifying system such as emulsion or cream, an ointment, a dispersion liquid and the like can be adopted.

[0028]

The external preparation for skin of the present invention synergistically improves the cell growth action. As a result, rough skin prevention and improvement effect,
It is possible to provide a skin external preparation having a high skin aging prevention effect or a wound healing effect and a good feeling in use.

[0029]

EXAMPLES Next, the present invention will be described in detail with reference to examples, but it goes without saying that the technical scope of the present invention is not limited to these examples.

[0030] First, glycyrrhizinic acid, a salt of glycyrrhizinic acid and glycyrrhizinate have the effects of the use of saikosaponins b ₁ and b ₂ on wound healing of skin, prevention and improvement of skin roughness, prevention of aging to prevent sagging and loss of skin, and a feeling of use. Acid derivatives, pantothenic acid, salts of pantothenic acid and pantothenic acid derivatives, spleen extract, allantoin, hinokitio-
Synergistically improved by blending the extract, placenta extract, vitamin C, vitamin C salt and vitamin C derivative, vitamin E, vitamin E salt and vitamin E derivative, hamamelis extract, collagen and carrot extract. In order to show that, the following tests were carried out for skin cell proliferation promoting effect, wound healing effect and stimulating (hemolytic effect) suppressing effect.

Skin Cell Proliferation-Promoting Action Human skin tissue is cut into small pieces, adhered to the bottom of a cell proliferation petri dish, and cultured in Dulbecco's MEM culture medium (containing 10% fetal calf serum) for 2 weeks. To be filled with fibroblasts. The fibroblasts were treated with 0.1% trypsin solution to give single cells, and 1000 cells / m
l of culture solution was prepared. 1m per dish of this solution
1, Dulbecco's MEM culture medium and various concentrations of saikosaponin b ₁ were added, or glycyrrhizic acid, pantothenyl ether, spleen extract,
Allantoin and placenta extract were added, and the cells were cultured in a CO ₂ incubator for 2 weeks. Then, the cells were fixed and stained, and the number of cell colonies was counted.

The results are shown in FIGS. The comparative example of FIG. 1 is the result when only saikosaponin b ₁ was added to the culture solution, and the example further added glycyrrhizic acid to ₁₀₀ p.
It is a result when pm is added. The comparative example in FIG. 2 is the result when only saikosaponin b ₁ was added to the culture solution,
In addition, in the examples, 1 to 1 of pantothenyl ether was added.
This is the result when 00 ppm was added. The comparative example in FIG. 3 is the result when only saikosaponin b ₁ was added to the culture solution, and the example further added 100 pp of spleen extract.
This is the result when m is added. The comparative example of FIG. 4 is the result when only saikosaponin b ₁ was added to the culture solution, and the example is the result when 100 ppm of allantoin was further added. The comparative example of FIG. 5 is the saikosaponin b ₁
Is the result when only 100% of the placenta extract was added, and the results of the Examples are the results when 100 ppm of the placenta extract was further added. The cell growth rate in the figure is based on the number of cells without the addition of saikosaponin b ₁ and expressed as a ratio.

As is apparent from FIGS. 1 to 5, the cell proliferation rate was significantly improved by adding glycyrrhizic acid, pantothenyl ethyl ether, spleen extract, allantoin and placenta extract. Similar results were obtained with saikosaponin b ₂ .

Wound healing effect test A group of 5 Wistar rats 6 weeks old was used as a group and subjected to a test after hair cutting. After anesthetizing the rat with Nemptal, the back skin was incised about 2 cm along the midline. Immediately, the incision was sutured with Mitsuheru, and Psychosaponin b ₁ 200
mg dissolved in 0.1 ml of physiological saline, or 200 mg of saikosaponin b _{1 in} 0.1 ml of physiological saline.
And glycyrrhizinic acid 50 mg dissolved in physiological saline 0.1 ml, saikosaponin b ₁ 20
A solution of 0 mg dissolved in 0.1 ml of physiological saline and 50 mg of pantotenyl ethyl ether was added to 0.1 m of physiological saline.
1 dissolved in 0.1 l, saikosaponin b ₁ 200 mg dissolved in physiological saline 0.1 ml, and spleen extract 5
0 mg dissolved in physiological saline 0.1 ml, Saikosaponin b ₁ 200 mg dissolved in physiological saline 0.1 ml, and allantoin 50 mg dissolved in physiological saline 0.1 m
which was dissolved in l, was applied once two weeks 1 day a solution obtained by dissolving saikosaponins b ₁ 200 mg physiological saline 0.1ml and the placenta 50mg those in saline 0.1ml. Two weeks later, the suture needle was removed, and cross section 1
A skin section was prepared so as to be cm. The cutting tension of this section was measured by using Tensilon UTM-4 manufactured by Toyo Sokki Co., Ltd.

While the average tension of only saikosaponin b ₁ was 470 g / cm, the average tension of the one to which glycyrrhizic acid was added was 498 g / cm, and the average tension of the one to which pantothenyl ether was added was 495 g / cm, the average tension of the spleen extract added was 500 g / cm, the average tension of the allantoin added was 505 g / cm, and the average tension of the placenta extract added was 503 g / c.
It was found that by adding glycyrrhizic acid, pantothenyl ether, spleen extract, allantoin and placenta extract, the wound healing effect of saikosaponin b ₁ was improved. Similar effects were also obtained with saikosaponin b ₂ .

Stimulation Suppressive Effect Test Erythrocytes were collected from human blood, and psychosaponin b ₁ or psychosaponin b ₁ and glycyrrhizic acid 100 μm were collected.
g, saikosaponin b ₁ and pantothenylethyl ether 100 μg, saikosaponin b ₁ and spleen extract 1
00μg, saikosaponin b ₁ and allantoin 100μ
g, saikosaponin b ₁ and placenta extract 100 μg
Was added for 1 hour and the amount of hemolysis was measured. The results obtained regarding the relationship between the saikosaponin b ₁ concentration and the hemolysis rate are shown in FIGS. 6 to 10.

As is clear from FIGS. 6 to 10, the hemolytic action of saikosaponin b ₁ was suppressed to a high concentration by adding glycyrrhizic acid, pantothenyl ether, spleen extract, allantoin and placenta extract. I understood it.

(Examples and Comparative Examples) A water phase and an alcohol phase having the compositions shown in Tables 1 and 2 were prepared, mixed and solubilized, and then filtered to produce a lotion.

[0038]

[Table 1]

[0039]

[Table 2]

[0040]Rough skin improvement effect  Using the lotions of Examples 1 to 13 and Comparative Example 1, human body panels
The skin roughening improvement effect test was conducted. The test method is as follows.
It is Ri.

The skin surface morphology of a healthy female person is obtained by replicating the skin using a replica method using myricin resin and observed with a microscope. Examples 1 to 13 and Comparative Example 1 were applied to the left and right sides of the face of each of 25 panels evaluated to have a rough skin condition 1 or 2 based on the criteria shown in Table 3 from the condition of the skin print and the peeling condition of the stratum corneum.
Lotion was applied once a day for 2 weeks.

After 2 weeks, the skin condition was observed again by the above replica method, and the skin condition was evaluated according to the criteria shown in Table 3. The results are summarized in Tables 4 and 5.

[0043]

[Table 3]

[0044]

[Table 4]

[0045]

[Table 5]

Stickiness and Irritation To each of the lotions of Examples 1 to 13, 25 testers actually applied the lotions, and the feeling of use (stickiness,
Sensory evaluation was made for irritation). For each sample,
The numbers of people who felt stickiness and irritation are shown in Tables 6 and 7.

[0047]

[Table 6]

[0048]

[Table 7]

As is clear from Tables 6 and 7, the lotion of this example exhibited a higher feeling of use than the comparative example.

(Examples 14 to 18) The external preparations for the skin prepared based on the compositions and the production methods shown in the following Examples all have an improved rough skin improving effect or a wound healing effect as compared with Comparative Examples, and also have an irritating property. Was also suppressed.

Example 14 Cream wt% (oil phase) Psychosaponin b ₂ 1.0 Cetostearyl alcohol 3.5 Squalane 38.0 Beeswax 3.8 Reduced lanolin 5.0 Ethyl stearate 4.0 Ethylparaben 0.3 Fragrance 0.03 (aqueous phase) Glycyrrhizic acid 0.01 Dipotassium glycyrrhizinate 0.01 Ethynyl estradiol 0.01 γ-Oryzanol 0.01 1,3-butylene glycol 2.0 Glycerin 2.0 Vitamin E 0.01 Ion-exchanged water 40.32

(Production method) The oil phase was melted by heating and kept at 75 ° C, and this was added to the aqueous phase heated to 75 ° C with stirring. Next, the mixture was uniformly emulsified with a homomixer and then rapidly cooled with stirring to obtain a cream.

Example 15 Emulsion wt% (Alcohol phase) Psychosaponin b ₂ 0.001 Perfume 0.03 Ethanol 3.0 (Oil phase) Stearic acid 1.5 Cetyl alcohol 0.5 Beeswax 2.0 Polyoxyethylene ( 10) oleyl alcohol 2.0 ethylparaben 0.3 (aqueous phase) pantothenic acid 5.0 pantothenic acid sodium salt 1.0 propylene glycol 3.0 nicotinic acid amide 0.01 ion-exchanged water 61.659 quince seed extract ( 5% aqueous solution) 20.0

(Production Method) An oil phase heated and melted at 70 ° C. was added to a 70 ° C. aqueous phase, preliminary emulsification was carried out, and further homogenized with a homomixer. While stirring this, the alcohol phase and the quince seed extract were added, and the mixture was cooled to 30 ° C. to obtain an emulsion.

Example 16 Pack Weight% Psychosaponin b ₁ 0.1 Spleen Extract Extract 1.0 Polyvinyl Alcohol 10.0 Polyethylene Glycol 3.0 Propylene Glycol 7.0 Ethanol 10.0 Methylparaben 0.05 Vitamin K 0.01 Vitamin C 0.01 Vitamin C sodium 0.01 Vitamin H 0.01 Fragrance 0.05 Loquat extract 0.01 Deionized water 68.75

(Production method) Polyethylene glycol, propylene glycol and methyl paraben were added to ion-exchanged water and dissolved by stirring. Next, add polyvinyl alcohol, heat and stir, and then saikosaponin b ₁ , flavor, vitamin K,
Ethanol in which vitamin H, loquat extract and spleen extract were dissolved was added and dissolved by stirring to obtain a pack.

Example 17 Cosmetic for scalp wt% (Phase A) Psychosaponin b ₁ 0.5 Allantoin 0.1 1,3-Butylene glycol 6.5 Polyethylene glycol 1500 5.0 Assembly extract 0.01 Ethanol 5. 5 Potassium hydroxide 0.05 Ion-exchanged water 41.67 (Phase B) Elastin 0.01 Keratin 0.01 Cystine 0.01 Cordyceps extract 0.01 Yukinoshita extract 0.01 2-hexyldecyl palmitate 10.0 Squalane 5.0 Butylparaben 0.3 Vitamin E Acetate 0.15 Fragrance 0.05 (Phase C) Apple Extract 0.01 Lemon Extract 0.01 Ion Exchange Water 25.4 Carboxyvinyl Polymer 0.2

(Manufacturing method) A solution obtained by dissolving phase B at 75 ° C.
It was added to Phase A heated to 75 ° C. with stirring, and then Phase C dissolved with stirring at room temperature was cooled with stirring to obtain a scalp cosmetic.

Example 18 Ointment wt% Saikosaponin b ₂ 5.0 Hinokitiol 10.0 Vaseline 40.0 Stearyl alcohol 4.5 Mokurou 20.0 Polyoxyethylene (10) oleyl alcohol 0.5 Rutin 0.01 Horse chestnut extract 0.01 Horsetail extract 0.01 Aloe extract 0.01 Ion-exchanged water 19.96

(Production method) Other components mixed and dissolved in 70 ° C. ion-exchanged water at 70 ° C. were added, and the mixture was uniformly emulsified with a homomixer, followed by cooling after emulsification to obtain an ointment.

Example 19 Emulsion wt% (alcohol phase) Saikosaponin b ₂ 0.001 Birch extract 0.01 Achillea millefolium extract 0.01 Time extract 0.01 Lily extract 0.01 Perfume 0.03 Ethanol 3.0 (oil Phase) Stearic acid 1.5 Cetyl alcohol 0.5 Beeswax 2.0 Polyoxyethylene (10) oleyl alcohol 2.0 Ethylparaben 0.3 (Aqueous phase) Placenta extract 5.0 Propylene glycol 3.0 Glycyrrhetinic acid Ion exchange Water 62.619 Quinceseed extract (5% aqueous solution) 20.0

(Production Method) An oil phase heated and melted at 70 ° C. was added to an aqueous phase at 70 ° C., preliminarily emulsified, and further homogenized with a homomixer. While stirring this, the alcohol phase and the quince seed extract were added, and the mixture was cooled to 30 ° C. to obtain an emulsion.

Example 20 Pack Weight% Psychosaponin b ₁ 0.1 L-Ascorbic acid-2-sodium sulfate 0.5 Magnesium ascorbyl phosphate 0.5 Polyvinyl alcohol 10.0 Polyethylene glycol 3.0 Propylene glycol 7. 0 ethanol 10.0 methylparaben 0.05 L-sodium glutamate 0.05 ion-exchanged water 68.8

(Production method) Polyethylene glycol, propylene glycol and methylparaben were added to ion-exchanged water and dissolved by stirring. Next, polyvinyl alcohol is added and heated and stirred, and ethanol in which psychosaponin b ₁ , fragrance, L-ascorbic acid-2-sodium sulfate, magnesium ascorbyl phosphate, and sodium L-glutamate are dissolved is added, stirred, dissolved, and packed. Got

Example 21 Cosmetic for scalp wt% (Phase A) Psychosaponin b ₁ 0.5 1,3-butylene glycol 6.5 Polyethylene glycol 1500 5.0 Ethanol 5.5 Potassium hydroxide 0.05 Ion exchange Water 41.95 (Phase B) Oat Extract 0.01 0.01 Burdock Extract 0.01 Luffa Extract 0.01 Otterswort Extract 0.01 Epidihydrocholesterine 0.01 Papain 0.01 2-Hexyldecyl Palmitate 10.0 Squalane 5. 0 butyl paraben 0.3 ethyl paraaminobenzoate 0.01 fragrance suitable amount vitamin E acetate 0.15 fragrance 0.05 (C phase) ion-exchanged water 25.4 carboxyvinyl polymer 0.2

(Manufacturing method) A solution obtained by dissolving phase B at 75 ° C.
It was added to Phase A heated to 75 ° C. with stirring, and then Phase C dissolved with stirring at room temperature was cooled with stirring to obtain a scalp cosmetic.

Example 22 Ointment wt% Saikosaponin b ₂ 5.0 Hamamelis Extract 5.0 Collagen 5.0 Vaseline 49.44 Stearyl Alcohol 4.5 Mokurou 20.0 Polyoxyethylene (10) Oleyl Alcohol 5 serine 0.01 dl-pyrrolidone carboxylic acid Na 0.01 chondroitin sulfate 0.01 hyaluronic acid 0.01 glycine 0.01 aspartic acid 0.01 ion-exchanged water 10.5

(Production method) Other components mixed and dissolved in 70 ° C. ion-exchanged water at 70 ° C. were added, homogenized with a homomixer, and after emulsification, cooled to obtain an ointment. Example 23 Cream wt% (oil phase) Psychosaponin b ₂ 1.0 Cetostearyl alcohol 3.5 Squalane 38.0 Beeswax 3.8 Reduced lanolin 5.0 Ethyl stearate 4.0 Ethylparaben 0.3 Perfume 0. 03 (water phase) Carrot extract L-arginine-L-aspartic acid 0.01 Rice extract 0.01 Iris extract 0.01 Cysteine 0.01 Diphenhydramine hydrochloride 0.01 Royal jelly extract 0.01 1,3 -Butylene glycol 2.0 Glycerin 2.0 Ion-exchanged water 40.3

(Production method) The oil phase was melted by heating and kept at 75 ° C, and this was added to the aqueous phase heated to 75 ° C with stirring. Next, the mixture was uniformly emulsified with a homomixer and then rapidly cooled with stirring to obtain a cream.

[0070]

[Brief description of drawings]

[0071]

FIG. 1 is a graph showing that the cell growth effect of saikosaponin b ₁ is improved by glycyrrhizic acid.

FIG. 2 is a graph showing that the cell growth effect of saikosaponin b ₁ is improved by pantothenyl ether.

FIG. 3 is a graph showing that the cell growth effect of saikosaponin b ₁ is improved by the spleen extract.

FIG. 4 is a graph showing that allantoin improves the cell growth effect of saikosaponin b ₁ .

FIG. 5 is a graph showing that the cell growth effect of saikosaponin b ₁ is improved by placenta extract,

FIG. 6 is a graph showing that erythrocyte hemolysis is suppressed by the addition of glycyrrhizic acid.

FIG. 7 is a graph showing that hemolysis of erythrocytes is suppressed by the addition of pantothenyl ethyl ether.

FIG. 8 is a graph showing that hemolysis of erythrocytes is suppressed by the addition of spleen extract.

FIG. 9 is a graph showing that hemolysis of red blood cells is suppressed by the addition of allantoin,

FIG. 10 is a graph showing that hemolysis of red blood cells is suppressed by the addition of placenta extract.

Continuation of the front page (51) Int.Cl. ⁵ Identification number Reference number within the agency FI Technical indication location A61K 7/00 F 9165-4C H 9165-4C 7/06 8615-4C 31/70 ABE ADA 8314-4C 37 / 02 ADT 8314-4C

Claims (2)

[Claims] 1. Saikosaponin b ₁ and / or saikosaponin b ₂ and glycyrrhizic acid, a salt of glycyrrhizic acid and a glycyrrhizic acid derivative, pantothenic acid, a salt of pantothenic acid and a pantothenic acid derivative, spleen extract, allantoin, hinokitiol. , Placenta extract, vitamin C, vitamin C salt and vitamin C derivative, vitamin E, vitamin E salt and vitamin E derivative, hamamelis extract, collagen, carrot extract, and L-arginine-L-aspartic acid. A skin external preparation characterized by containing one or more kinds. 2. _One or two kinds selected from the saikosaponin b ₁ and / or the saikosaponin b ₂ is 0.000.
The external preparation for skin according to claim 1, wherein the external preparation contains 1 to 20% by weight.