JPH0440654B2 - - Google Patents
Info
- Publication number
- JPH0440654B2 JPH0440654B2 JP59038311A JP3831184A JPH0440654B2 JP H0440654 B2 JPH0440654 B2 JP H0440654B2 JP 59038311 A JP59038311 A JP 59038311A JP 3831184 A JP3831184 A JP 3831184A JP H0440654 B2 JPH0440654 B2 JP H0440654B2
- Authority
- JP
- Japan
- Prior art keywords
- methane
- fluorescence
- wavelength range
- bacteria
- methane bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 164
- 241000894006 Bacteria Species 0.000 claims description 52
- 230000000694 effects Effects 0.000 claims description 27
- 230000005284 excitation Effects 0.000 claims description 25
- 238000004519 manufacturing process Methods 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 12
- 238000005259 measurement Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 description 21
- 238000000695 excitation spectrum Methods 0.000 description 18
- 239000000126 substance Substances 0.000 description 16
- 238000002189 fluorescence spectrum Methods 0.000 description 15
- 230000004151 fermentation Effects 0.000 description 12
- 238000000855 fermentation Methods 0.000 description 12
- 239000010802 sludge Substances 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000013307 optical fiber Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000037149 energy metabolism Effects 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 230000000696 methanogenic effect Effects 0.000 description 3
- 229950006238 nadide Drugs 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 241001074903 Methanobacteria Species 0.000 description 2
- 241000205275 Methanosarcina barkeri Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000003637 basic solution Substances 0.000 description 2
- 230000027721 electron transport chain Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108091006149 Electron carriers Proteins 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000202974 Methanobacterium Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59038311A JPS60181637A (ja) | 1984-02-28 | 1984-02-28 | メタン菌の菌数またはメタン生成活性の測定方法 |
| PCT/JP1984/000230 WO1984004544A1 (en) | 1983-05-09 | 1984-05-07 | Method for measuring the number or methane-producing activity of methane bacteria |
| DE19843490229 DE3490229T1 (de) | 1983-05-09 | 1984-05-07 | Verfahren zum Messen der Zellenzahl oder methanerzeugender Aktivität von Methanogenen |
| US06/694,384 US4686372A (en) | 1983-05-09 | 1984-05-07 | Method and apparatus for measuring cell counts of Methanogens or methane producing activity thereof |
| GB08505078A GB2155631B (en) | 1984-02-28 | 1985-02-27 | Measurements on methane-producing organisms |
| FR858502843A FR2560214B1 (fr) | 1984-02-28 | 1985-02-27 | Procede de mesure du nombre de cellules de methanogenes ou de leur activite de production de methane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59038311A JPS60181637A (ja) | 1984-02-28 | 1984-02-28 | メタン菌の菌数またはメタン生成活性の測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60181637A JPS60181637A (ja) | 1985-09-17 |
| JPH0440654B2 true JPH0440654B2 (zh) | 1992-07-03 |
Family
ID=12521747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59038311A Granted JPS60181637A (ja) | 1983-05-09 | 1984-02-28 | メタン菌の菌数またはメタン生成活性の測定方法 |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS60181637A (zh) |
| FR (1) | FR2560214B1 (zh) |
| GB (1) | GB2155631B (zh) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60220199A (ja) * | 1984-04-13 | 1985-11-02 | Sumitomo Heavy Ind Ltd | メタン発酵処理方法 |
| DE3811098A1 (de) * | 1988-03-31 | 1989-10-12 | Orpegen Med Molekularbioforsch | Verfahren zur quantifizierung von methangas-bakterien |
| JPH03127697A (ja) * | 1989-10-09 | 1991-05-30 | Shimizu Corp | 嫌気性発酵による廃水処理制御方法及び装置 |
| JP5159140B2 (ja) * | 2007-03-30 | 2013-03-06 | 株式会社クボタ | 有機性廃棄物の処理方法および装置 |
| CN103102056B (zh) * | 2013-03-13 | 2014-03-12 | 南京盟博环保科技有限公司 | 一种用于污泥减水减量的设备 |
| CN103364382A (zh) * | 2013-07-12 | 2013-10-23 | 大连海事大学 | 一种船舶生活污水检测装置 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3566114A (en) * | 1968-04-25 | 1971-02-23 | Aubrey K Brewer | Method and means for detection of microorganisms in the atmosphere |
| US3916197A (en) * | 1973-11-28 | 1975-10-28 | Particle Technology Inc | Method and apparatus for classifying biological cells |
| US4283490A (en) * | 1978-07-28 | 1981-08-11 | Plakas Chris J | Method for detection of low level bacterial concentration by luminescence |
-
1984
- 1984-02-28 JP JP59038311A patent/JPS60181637A/ja active Granted
-
1985
- 1985-02-27 GB GB08505078A patent/GB2155631B/en not_active Expired
- 1985-02-27 FR FR858502843A patent/FR2560214B1/fr not_active Expired
Non-Patent Citations (1)
| Title |
|---|
| BIOGAS-METHANGARUNG ORGANISCHER ABFALLSTOFFE=1982 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60181637A (ja) | 1985-09-17 |
| GB2155631A (en) | 1985-09-25 |
| FR2560214B1 (fr) | 1989-09-29 |
| FR2560214A1 (fr) | 1985-08-30 |
| GB8505078D0 (en) | 1985-03-27 |
| GB2155631B (en) | 1988-01-06 |
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