JPH04370197A - Enzymatic liquid cleaning agent - Google Patents
Enzymatic liquid cleaning agentInfo
- Publication number
- JPH04370197A JPH04370197A JP14457891A JP14457891A JPH04370197A JP H04370197 A JPH04370197 A JP H04370197A JP 14457891 A JP14457891 A JP 14457891A JP 14457891 A JP14457891 A JP 14457891A JP H04370197 A JPH04370197 A JP H04370197A
- Authority
- JP
- Japan
- Prior art keywords
- weight
- parts
- liquid detergent
- pts
- lipolytic enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims description 25
- 230000002255 enzymatic effect Effects 0.000 title claims description 10
- 239000012459 cleaning agent Substances 0.000 title abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 39
- 108090000790 Enzymes Proteins 0.000 claims abstract description 39
- 150000005846 sugar alcohols Polymers 0.000 claims abstract description 8
- 150000001639 boron compounds Chemical class 0.000 claims abstract description 7
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims abstract description 7
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims abstract description 7
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 3
- 239000003381 stabilizer Substances 0.000 claims abstract 3
- 239000003599 detergent Substances 0.000 claims description 18
- 230000002366 lipolytic effect Effects 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 5
- 241000588881 Chromobacterium Species 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 abstract description 9
- 239000000243 solution Substances 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract 1
- 239000002798 polar solvent Substances 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 20
- 230000000694 effects Effects 0.000 description 18
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 15
- 235000011187 glycerol Nutrition 0.000 description 10
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 9
- 239000004327 boric acid Substances 0.000 description 9
- 238000004140 cleaning Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 5
- 235000010241 potassium sorbate Nutrition 0.000 description 5
- 239000004302 potassium sorbate Substances 0.000 description 5
- 229940069338 potassium sorbate Drugs 0.000 description 5
- 239000000600 sorbitol Substances 0.000 description 5
- 101710084373 Lipase 1 Proteins 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 235000010199 sorbic acid Nutrition 0.000 description 3
- 239000004334 sorbic acid Substances 0.000 description 3
- 229940075582 sorbic acid Drugs 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 101710084378 Lipase 2 Proteins 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- FLPJVCMIKUWSDR-UHFFFAOYSA-N 2-(4-formylphenoxy)acetamide Chemical compound NC(=O)COC1=CC=C(C=O)C=C1 FLPJVCMIKUWSDR-UHFFFAOYSA-N 0.000 description 1
- 241000146387 Chromobacterium viscosum Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 239000004280 Sodium formate Substances 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 229940074979 cetyl palmitate Drugs 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008202 granule composition Substances 0.000 description 1
- PXDJXZJSCPSGGI-UHFFFAOYSA-N hexadecanoic acid hexadecyl ester Natural products CCCCCCCCCCCCCCCCOC(=O)CCCCCCCCCCCCCCC PXDJXZJSCPSGGI-UHFFFAOYSA-N 0.000 description 1
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 description 1
- 238000011086 high cleaning Methods 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 1
- 235000019254 sodium formate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Landscapes
- Eyeglasses (AREA)
- Detergent Compositions (AREA)
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は洗浄用または浸漬洗浄用
の組成物に適する安定化した酵素液体洗浄剤に関するも
のである。さらに特定的には、本発明は水性の脂質分解
酵素を含む液体洗浄剤に関するものであり、これは特に
コンタクトレンズの汚れを安全に確実に除去できること
を特徴とする。FIELD OF THE INVENTION This invention relates to stabilized enzymatic liquid detergents suitable for cleaning or immersion cleaning compositions. More particularly, the present invention relates to a liquid cleaning agent containing an aqueous lipolytic enzyme, which is particularly characterized by its ability to safely and reliably remove stains from contact lenses.
【０００２】0002
【従来の技術】酵素の液体中での安定化は従来から多大
なる関心の的であったが、水溶液中では不安定であるこ
とが知られている。洗浄剤への酵素の添加は、界面活性
剤では落ちにくい汚染布上の脂肪分やリポ蛋白質を分解
し、それによって血液汚染，肉汁汚染，体液汚染等を除
去可能にする有効性に起因する。特にコンタクトレンズ
の脂質汚れには有効である。しかし、酵素は洗浄剤成分
の中では比較的高価であり、また洗浄効果も高いが保存
安定性が悪く有効に活用できない。特に液状では尚さら
のことである。従来、脂質分解酵素を含む洗浄剤は粉末
または顆粒状組成物として利用されているが、溶解性が
悪い、洗浄時間が長い、操作が頻雑等の問題がある。し
かし、上記洗浄剤は脂質汚れの除去には非常に有効であ
る。例えば、特公昭５９−１８７１００号公報、特公昭
６０−１２１４１７号公報等に開示されている。BACKGROUND OF THE INVENTION The stabilization of enzymes in liquids has been the subject of great interest, but it is known that enzymes are unstable in aqueous solutions. The addition of enzymes to cleaning agents is due to their effectiveness in decomposing fat and lipoproteins on contaminated cloth that are difficult to remove with surfactants, thereby making it possible to remove blood contamination, meat juice contamination, body fluid contamination, etc. It is particularly effective against lipid stains on contact lenses. However, enzymes are relatively expensive among cleaning agent components, and although they have a high cleaning effect, they have poor storage stability and cannot be used effectively. This is especially true in liquid form. Conventionally, cleaning agents containing lipolytic enzymes have been used in the form of powder or granule compositions, but these have problems such as poor solubility, long cleaning times, and frequent operations. However, the above detergents are very effective in removing lipid stains. For example, it is disclosed in Japanese Patent Publication No. 59-187100, Japanese Patent Publication No. 60-121417, etc.
【０００３】0003
【発明が解決しようとする課題】しかしながら、特公昭
５９−１８７１００号公報、特公昭６０−１２１４１７
号公報等記載の方法の内、酵素を主成分としている用時
溶解タイプの洗浄剤では調整後、ほとんど数十時間以内
に酵素活性が消失してしまう。また、特公平２−２２７
５００号公報、特公平２−２７６８９９号公報に記載さ
れている脂質分解酵素の液体中での安定性は十分でなく
、さらに生体に対して危険性のあるギ酸ナトリウムや１
，２−ベンゼオ−ルを用いており好ましくない。本発明
は、これらの問題を解決することを課題として鋭意研究
を行い到達したものである。Problem to be Solved by the Invention However, Japanese Patent Publications No. 59-187100 and Japanese Patent Publication No. 60-121417
Among the methods described in the above-mentioned publications, the enzyme activity disappears within several tens of hours after preparation with a type of detergent that dissolves at the time of use, which contains enzymes as a main component. Also, Tokuhei 2-227
The lipid-degrading enzymes described in Japanese Patent Publication No. 500 and Japanese Patent Publication No. 2-276899 do not have sufficient stability in liquids, and they also contain sodium formate and
, 2-benzeol is used, which is not preferable. The present invention was achieved through intensive research aimed at solving these problems.
【０００４】0004
【課題を解決するための手段】本発明は、脂質分解酵素
と一定の条件の多価アルコ−ル混合組成により本目的が
達成されることを見いだした．多価アルコ−ル混合組成
は脂質分解酵素の液体中での安定性を飛躍的に高め、ホ
ウ素化合物はその効果をさらに高める。本発明で用いる
脂質分解酵素は、Ｃｈｒｏｍｏｂａｃｔｅｒｉｕｍ
ｖｉｓ−ｃｏｓｕｍ起源のものが用いられ０．０１〜１
０重量部が含有される。０．０１重量部以下では十分効
果が現れず、また１０重量部以上では溶解性が悪い。[Means for Solving the Problems] The present invention has found that the object can be achieved by a mixed composition of a lipolytic enzyme and a polyhydric alcohol under certain conditions. The polyhydric alcohol mixed composition dramatically increases the stability of the lipolytic enzyme in liquid, and the boron compound further enhances the effect. The lipolytic enzyme used in the present invention is Chromobacterium
vis-cosum origin is used and 0.01 to 1
Contains 0 parts by weight. If the amount is less than 0.01 parts by weight, the effect will not be sufficient, and if it is more than 10 parts by weight, the solubility will be poor.
【０００５】本発明で用いる多価アルコ−ルは、生体に
対する安全性が高く、且つ水溶解性の良いものが好まし
い。例えば、ポリエチレングリコ−ル、エチレングリコ
−ル、プロピレングリコ−ル、グリセリン、キシリト−
ル、ソルビト−ル、マンニト−ル、トレオ−ス、グリセ
リン、グリセロリン酸塩、アスコルビン酸、ヘキサンジ
オ−ル、デキストリン等から選択される。この多価アル
コ−ルの使用量は、５０重量部以上が良く、好ましくは
７０〜９０重量部が安定性上最も良い。５０重量部以下
では安定性が十分でなく、２０重量部以下では効果がほ
とんどない。本発明で用いるホウ素化合物は、生体に対
して安全であり、防腐効力を有しているものが用いられ
る。例えば、ホウ酸、ホウ砂等がある。このホウ素化合
物の使用量は０．０１〜１．０重量部が良い。この範囲
以外では十分な安定性が得られない。The polyhydric alcohol used in the present invention is preferably one that is highly safe for living organisms and has good water solubility. For example, polyethylene glycol, ethylene glycol, propylene glycol, glycerin, xylitol
sorbitol, mannitol, threose, glycerin, glycerophosphate, ascorbic acid, hexanediol, dextrin and the like. The amount of polyhydric alcohol used is preferably 50 parts by weight or more, preferably 70 to 90 parts by weight, which is best for stability. If it is less than 50 parts by weight, stability is insufficient, and if it is less than 20 parts by weight, there is almost no effect. The boron compound used in the present invention is one that is safe for living organisms and has an antiseptic effect. Examples include boric acid and borax. The amount of this boron compound used is preferably 0.01 to 1.0 parts by weight. Sufficient stability cannot be obtained outside this range.
【０００６】本発明で用いるポリビニルピロリドンの使
用量は０．０５〜５．０重量部が良い。０．０５重量部
以下では良好な安定性が示めされず、また５．０重量部
以上では溶解性の点で好ましくない。The amount of polyvinylpyrrolidone used in the present invention is preferably 0.05 to 5.0 parts by weight. If it is less than 0.05 parts by weight, good stability is not exhibited, and if it is more than 5.0 parts by weight, it is unfavorable from the viewpoint of solubility.
【０００７】本発明の酵素液体洗浄剤は、これらの成分
以外に従来既知の成分を含むこともできる。すなわち、
界面活性剤、高分子化合物、防腐剤、キレ−ト剤、ｐＨ
調製剤、無機塩等を含むことができる。The enzymatic liquid detergent of the present invention may also contain conventionally known components in addition to these components. That is,
Surfactant, polymer compound, preservative, chelating agent, pH
Preparative agents, inorganic salts, etc. can be included.
【０００８】本発明の酵素液体洗浄剤は、以上のように
して得られた溶液タイプの酵素洗浄剤である為、使用時
はケ−ス等に１滴〜数滴を入れ、さらに生理食塩水や特
定の希釈剤で希釈して浸漬処理して用いる。処理後適量
のすすぎ液ですすいで洗浄を終了する。Since the enzyme liquid detergent of the present invention is a solution-type enzyme detergent obtained as described above, when using it, put one to several drops into a case, etc., and then add physiological saline solution. It is used by diluting with a specific diluent and immersion treatment. After processing, rinse with an appropriate amount of rinsing liquid to complete cleaning.
【０００９】[0009]
【作用】本発明による酵素液体洗浄剤は、特定の脂質分
解酵素と特定組成の多価アルコ−ルを含んだ溶剤が、脂
質分解酵素を長期間液状で安定に保つことできる。また
ホウ素化合物またはポリビニルピロリドンがその効果を
高め、さらには酵素の作用により脂質汚れが効率よく除
去できる。[Function] In the enzymatic liquid detergent according to the present invention, the solvent containing a specific lipolytic enzyme and a polyhydric alcohol of a specific composition can keep the lipolytic enzyme stable in a liquid state for a long period of time. In addition, boron compounds or polyvinylpyrrolidone enhance the effect, and furthermore, lipid stains can be efficiently removed by the action of enzymes.
【００１０】0010
（実施例１）
グリセリン ６０
重量部プロピレングリコ−ル
５ 重量部ホウ酸
０．０５ 重量
部ソルビン酸カリウム ０．０２
５重量部リパ−ゼ
１ 重量部ソルビト−ル
１ 重量
部ポリビニルピロリドンｋ２５ ０．２５ 重量
部水
３０ 重量部４０℃、４週間後の酵
素活性は７５％以上で良好な洗浄力を示した。(Example 1) Glycerin 60
Part by weight propylene glycol
5 parts by weight boric acid
0.05 Part by weight Potassium sorbate 0.02
5 parts by weight lipase
1 part by weight sorbitol
1 part by weight Polyvinylpyrrolidone k25 0.25 part by weight Water
After 4 weeks at 40° C., the enzyme activity was 75% or more, indicating good detergency.
【００１１】（実施例２）
グリセリン ６０
重量部プロピレングリコ−ル
１０ 重量部ホウ酸
０．０５ 重量部
ソルビン酸カリウム ０．０２５
重量部リパ−ゼ
２ 重量部ポリビニルピロリドン
ｋ２５ ０．５ 重量部水
３０
重量部４０℃、４週間後の酵素活性は８２％以上で
良好な洗浄力を示した。(Example 2) Glycerin 60
Part by weight propylene glycol
10 parts by weight boric acid
0.05 Part by weight Potassium sorbate 0.025
Part by weight lipase
2 parts by weight Polyvinylpyrrolidone k25 0.5 parts by weight Water
30
The enzyme activity after 4 weeks at 40° C. by weight was 82% or more, indicating good detergency.
【００１２】（実施例３）
グリセリン ７０
重量部ホウ酸
０．０５ 重量部ソルビン酸
０．０２５重
量部リパ−ゼ
１ 重量部ソルビト−ル
１．５ 重量部水
２０
重量部４０℃、４週間後の酵素活性は
８１％以上で良好な洗浄力を示した。(Example 3) Glycerin 70
Part by weight boric acid
0.05 parts by weight sorbic acid 0.025 parts by weight lipase
1 part by weight sorbitol
1.5 parts by weight water
20
The enzyme activity after 4 weeks at 40° C. by weight was 81% or more, indicating good detergency.
【００１３】（実施例４）
グリセリン ７０
重量部プロピレングリコ−ル
３０ 重量部ホウ酸
０．０５ 重量部
ソルビン酸 ０
．０２５重量部リパ−ゼ
１ 重量部水
２０
重量部４０℃、４週間後の酵素活性は７０％
以上で良好な洗浄力を示した。(Example 4) Glycerin 70
Part by weight propylene glycol
30 parts by weight boric acid
0.05 parts by weight Sorbic acid 0
．． 025 parts by weight lipase
1 part by weight water
20
Weight part Enzyme activity after 4 weeks at 40°C is 70%
The above results showed good cleaning power.
【００１４】（比較例１）
グリセリン ２０
重量部ホウ酸
０．０５ 重量部ソルビン酸
０．０２５重
量部リパ−ゼ
１ 重量部水
７５
重量部４０℃、４週間後の酵素活性は検出できず実用上
不十分であった。(Comparative Example 1) Glycerin 20
Part by weight boric acid
0.05 parts by weight sorbic acid 0.025 parts by weight lipase
1 part by weight water
75
The enzyme activity after 4 weeks at 40° C. by weight could not be detected and was insufficient for practical use.
【００１５】（比較例２）
グリセリン ４５
重量部ホウ酸
０．０５ 重量部ソルビン酸
カリウム ０．０２５重量部リパ
−ゼ ２
重量部ポリビニルピロリドンｋ２５
１ 重量部ソルビト−ル
１．０ 重量部水
５５
重量部４０℃、４週間後の酵素活性は２
８％であり、洗浄力も十分でなかった。(Comparative Example 2) Glycerin 45
Part by weight boric acid
0.05 parts by weight Potassium sorbate 0.025 parts by weight Lipase 2
Weight part polyvinylpyrrolidone k25
1 part by weight sorbitol
1.0 parts by weight water
55
After 4 weeks at 40°C, the enzyme activity was 2.
It was 8%, and the detergency was not sufficient.
【００１６】（比較例３）
グリセリン ７０
重量部ホウ酸
０．０５ 重量部ソルビン酸
カリウム ０．０２５重量部糸状
菌由来リパ−ゼ １
重量部ソルビト−ル
１．５ 重量部水
２０ 重
量部４０℃、４週間後の酵素活性は検出できず実用上不
十分であった。(Comparative Example 3) Glycerin 70
Part by weight boric acid
0.05 parts by weight Potassium sorbate 0.025 parts by weight Lipase derived from filamentous fungi 1
Part by weight sorbitol
1.5 parts by weight water
20 parts by weight at 40°C for 4 weeks, the enzyme activity could not be detected and was insufficient for practical use.
【００１７】（比較例４）
グリセリン ５０
重量部プロピレングリコ−ル
３０ 重量部ホウ酸
０．１ 重量
部ソルビン酸カリウム ０．０２
５重量部糸状菌由来リパ−ゼ １
重量部水
２０ 重量部
４０℃、４週間後の酵素活性は検出できず実用上不十分
であった。(Comparative Example 4) Glycerin 50
Part by weight propylene glycol
30 parts by weight boric acid
0.1 Part by weight Potassium sorbate 0.02
5 parts by weight lipase derived from filamentous fungi 1
Part by weight water
20 parts by weight at 40°C for 4 weeks, the enzyme activity could not be detected and was insufficient for practical use.
【００１８】〈評価基準〉脂質分解酵素活性は、薬審第
５２３号脂肪消化力試験法に準拠して測定した。洗浄効
力テストは人工脂質溶液（パルミチン酸セチル０．０１
ｇ，コレステロ−ル０．０２ｇ，レシチン０．０５ｇ，
オリ−ブ油０．１ｇ，ポリエチレングリコ−ル１０ｇを
生理食塩水に懸濁して１００ｍｌとした）にコンタクト
レンズ（セイコ−コンタクトレンズ製、ポリコンＨＤｋ
）を浸し、６０℃に加熱後冷却して脂質を付着させたレ
ンズを用いて試験した。酵素液体洗浄剤１滴をコンタク
トレンズ用保存液（セイコ−ハ−ド用保存液）２．０ｍ
ｌで希釈し２時間処理後、実体顕微鏡にて付着脂質がレ
ンズ全体の１０％未満の場合良好とした。<Evaluation Criteria> Lipid-degrading enzyme activity was measured in accordance with the Fat Digestion Test Method No. 523 of the Japanese Pharmaceutical Affairs Authority. The cleaning efficacy test was performed using an artificial lipid solution (cetyl palmitate 0.01
g, cholesterol 0.02g, lecithin 0.05g,
0.1 g of olive oil and 10 g of polyethylene glycol were suspended in physiological saline to make 100 ml) and a contact lens (manufactured by Seiko Contact Lenses, Polycon HDk)
), heated to 60°C, cooled, and then tested using a lens to which lipids were attached. Add 1 drop of enzyme liquid detergent to 2.0 m of contact lens storage solution (Seiko hard storage solution)
After treatment for 2 hours, the lens was evaluated as good if the amount of attached lipid was less than 10% of the entire lens under a stereomicroscope.
【００１９】[0019]
【発明の効果】本発明は、液状での脂質分解酵素の保存
安定性を、生体に対して安全な溶剤系にて飛躍的に高め
ることで液状の脂質分解酵素洗浄剤を可能とし、さらに
より安全でより優れた洗浄効果が得られる。また、この
洗浄剤を用いることによりコンタクトレンズ等において
、擦り洗浄ケアから浸漬洗浄への変更を可能とし、レン
ズ等の破損，損失の問題がなくなるという効果もある。[Effects of the Invention] The present invention dramatically increases the storage stability of lipolytic enzymes in liquid form using a solvent system that is safe for living organisms, thereby making possible a liquid lipolytic enzyme detergent. Safe and better cleaning effect can be obtained. Furthermore, by using this cleaning agent, it is possible to switch from rubbing cleaning care to immersion cleaning for contact lenses, etc., and there is also the effect that problems of damage and loss of lenses, etc. are eliminated.
Claims (5)
５重量部の多価アルコ−ルを含む水溶液に均一に溶解分
散させ、安定化された液体であることを特徴とする酵素
液体洗浄剤。Claim 1: A lipolytic enzyme containing at least 50 to 9
An enzyme liquid detergent characterized by being a stabilized liquid that is uniformly dissolved and dispersed in an aqueous solution containing 5 parts by weight of polyhydric alcohol.
酵素が、Ｃｈｒｏｍｏ−ｂａｃｔｅｒｉｕｍ ｖｉｓ
ｃｏｓｕｍ起源の脂質分解酵素であることを特徴とする
請求項１記載の酵素液体洗浄剤。2. The lipolytic enzyme used in the liquid detergent is Chromo-bacterium vis
The enzymatic liquid detergent according to claim 1, which is a lipolytic enzyme derived from cosum.
酵素を、最終組成物の０．０１〜１０％含むことを特徴
とする請求項１記載の酵素液体洗浄剤。3. The enzymatic liquid detergent according to claim 1, wherein the lipolytic enzyme used in the liquid detergent contains 0.01 to 10% of the final composition.
安定化剤としてホウ素化合物を０．０１〜１．０重量部
含有することを特徴とする請求項１記載の酵素液体洗浄
剤。4. The enzymatic liquid detergent according to claim 1, wherein the enzymatic liquid detergent contains 0.01 to 1.0 parts by weight of a boron compound as a lipolytic enzyme stabilizer.
安定化剤としてホウ素化合物を０．０１〜１．０重量部
及びポリビニルピロリドンを０．０５〜５．０重量部含
有することを特徴とする請求項１記載の酵素液体洗浄剤
。5. The enzymatic liquid detergent contains 0.01 to 1.0 parts by weight of a boron compound and 0.05 to 5.0 parts by weight of polyvinylpyrrolidone as a lipolytic enzyme stabilizer. The enzymatic liquid detergent according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14457891A JPH04370197A (en) | 1991-06-17 | 1991-06-17 | Enzymatic liquid cleaning agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14457891A JPH04370197A (en) | 1991-06-17 | 1991-06-17 | Enzymatic liquid cleaning agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04370197A true JPH04370197A (en) | 1992-12-22 |
Family
ID=15365419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14457891A Pending JPH04370197A (en) | 1991-06-17 | 1991-06-17 | Enzymatic liquid cleaning agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04370197A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996025957A1 (en) * | 1995-02-21 | 1996-08-29 | Tomey Technology Corporation | Cleaning and disinfecting agent for contact lens and method for cleaning and disinfecting contact lens using the same |
| US5576278A (en) * | 1995-06-07 | 1996-11-19 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use |
| US5604190A (en) * | 1995-06-07 | 1997-02-18 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5605661A (en) * | 1995-08-18 | 1997-02-25 | Alcon Laboratories, Inc. | Methods of using liquid enzyme compositions containing mixed polyols |
| US5672213A (en) * | 1995-08-18 | 1997-09-30 | Alcon Laboratories, Inc. | Liquid enzyme compositions containing aromatic acid derivatives |
| US5723421A (en) * | 1995-06-07 | 1998-03-03 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6139646A (en) * | 1998-09-01 | 2000-10-31 | Alcon Laboratories, Inc. | Alkyl trypsin compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6184189B1 (en) | 1995-06-07 | 2001-02-06 | Alcon Laboratories, Inc. | Liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6214596B1 (en) | 1996-12-18 | 2001-04-10 | Alcon Laboratories, Inc. | Liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6228323B1 (en) | 1996-12-13 | 2001-05-08 | Alcon Laboratories, Inc. | Multi-purpose compositions containing an alkyl-trypsin and methods of use in contact lens cleaning and disinfecting |
| WO2001038471A1 (en) * | 1999-11-24 | 2001-05-31 | Henkel Kommanditgesellschaft Auf Aktien | Method for production of enzyme-containing micro-emulsions and micro-encapsulated enzymes |
| US11150383B2 (en) | 2005-02-14 | 2021-10-19 | Johnson & Johnson Vision Care, Inc. | Comfortable ophthalmic device and methods of its production |
-
1991
- 1991-06-17 JP JP14457891A patent/JPH04370197A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5785767A (en) * | 1995-02-21 | 1998-07-28 | Tomey Technology Corporation | Cleaning and disinfecting solution for contact lens and method of cleaning and disinfecting contact lens using the solution |
| WO1996025957A1 (en) * | 1995-02-21 | 1996-08-29 | Tomey Technology Corporation | Cleaning and disinfecting agent for contact lens and method for cleaning and disinfecting contact lens using the same |
| US6184189B1 (en) | 1995-06-07 | 2001-02-06 | Alcon Laboratories, Inc. | Liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5723421A (en) * | 1995-06-07 | 1998-03-03 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5604190A (en) * | 1995-06-07 | 1997-02-18 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5939369A (en) * | 1995-06-07 | 1999-08-17 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5948738A (en) * | 1995-06-07 | 1999-09-07 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US5576278A (en) * | 1995-06-07 | 1996-11-19 | Alcon Laboratories, Inc. | Stable liquid enzyme compositions and methods of use |
| US5672213A (en) * | 1995-08-18 | 1997-09-30 | Alcon Laboratories, Inc. | Liquid enzyme compositions containing aromatic acid derivatives |
| US5919313A (en) * | 1995-08-18 | 1999-07-06 | Alcon Laboratories, Inc. | Liquid enzyme compositions containing aromatic acid derivatives and methods of use |
| US6069120A (en) * | 1995-08-18 | 2000-05-30 | Alcon Laboratories, Inc. | Liquid enzyme compositions containing mixed polyols and methods of use |
| US5605661A (en) * | 1995-08-18 | 1997-02-25 | Alcon Laboratories, Inc. | Methods of using liquid enzyme compositions containing mixed polyols |
| US6358897B1 (en) | 1996-06-07 | 2002-03-19 | Alcon Laboratories, Inc. | Alkyl trypsin compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6228323B1 (en) | 1996-12-13 | 2001-05-08 | Alcon Laboratories, Inc. | Multi-purpose compositions containing an alkyl-trypsin and methods of use in contact lens cleaning and disinfecting |
| US6214596B1 (en) | 1996-12-18 | 2001-04-10 | Alcon Laboratories, Inc. | Liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems |
| US6139646A (en) * | 1998-09-01 | 2000-10-31 | Alcon Laboratories, Inc. | Alkyl trypsin compositions and methods of use in contact lens cleaning and disinfecting systems |
| WO2001038471A1 (en) * | 1999-11-24 | 2001-05-31 | Henkel Kommanditgesellschaft Auf Aktien | Method for production of enzyme-containing micro-emulsions and micro-encapsulated enzymes |
| US11150383B2 (en) | 2005-02-14 | 2021-10-19 | Johnson & Johnson Vision Care, Inc. | Comfortable ophthalmic device and methods of its production |
| US11953651B2 (en) | 2005-02-14 | 2024-04-09 | Johnson & Johnson Vision Care, Inc. | Comfortable ophthalmic device and methods of its production |
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