JPH04238251A - Cell blank measuring method - Google Patents

Abstract

PURPOSE:To automatically perform the cell blank measurement of a reaction container already progressed with a reaction when no cell blank measurement can be performed due to a failure of a cleaning device and the like in the cell blank measurement of an automatic analyzer. CONSTITUTION:The cell blank measurement of an automatic analyzer is performed for a reaction container storing the cleaning water at the same wavelength as the analysis wavelength after the reaction container is cleaned, if no cell blank measurement can be performed due to a failure of a cleaning device and the like, the cell blank value of the previous reaction container is stored for the whole wavelength, and the cell blank measurement of the reaction container already progressed with a reaction is performed with this cell blank value.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell blank measurement method in an automatic analyzer configured to clean a reaction vessel and reuse it.

0002

[Prior Art] It is well known that accurate analytical data cannot be obtained in an automatic analyzer configured to clean a reaction container for reuse unless the absorbance of the reaction container itself is canceled out. The cell blank value of the reaction vessel is particularly important in the endo method, which directly determines the concentration.

[0003] Currently, in conventional automatic analyzers, the cell blank value is measured at a stage before the sample and reagent are dispensed, that is, at a cleaning position before sampling is performed. .

That is, in conventional cell blank value measurements, for example, cell blank values are measured at all of the 12 measurement wavelengths used in general automatic analyzers, without considering the measurement wavelengths set for each analysis item of the sample. Currently, blank values are determined and the average value of all these cell blank values is used as the cell blank value. This is because the analysis item cannot be specified before the specimen is sampled, and therefore the analysis wavelength cannot be specified either.

[0005] However, although it is ideal that the cell blank value of the reaction vessel is measured at a wavelength corresponding to a specific wavelength during analysis, in the above conventional cell blank measurement method, Since cell blank values measured at wavelengths unrelated to the analysis wavelength are also used as the basis for calculating the average value, measurement accuracy tends to vary, especially in the low value region, and reliability in measurement accuracy cannot be maintained. There was a problem.

In order to solve these problems, a cell blank measurement method has been proposed, for example, as disclosed in Japanese Patent Application Laid-Open No. 2-278155.

[0007] This conventional cell blank measurement method attempts to solve the above problem by determining a cell blank value by measuring the washed reaction vessel at the same wavelength as the analysis wavelength after the analysis process is completed. It is something.

[0008]

[Problem to be Solved by the Invention] However, in the conventional method of measuring a cell blank after the analysis process is completed, cleaning water overflows in the cleaning stage due to a failure of the cleaning equipment, making cleaning impossible. In some cases, cell blank measurements cannot be performed on reaction vessels in which reactions have already progressed, resulting in poor reliability in measurement accuracy.
There was a problem.

[0009] The present invention was devised in view of the current situation, and its purpose is to eliminate the problem even when washing water overflows in the washing stage due to a failure of the washing equipment and washing becomes impossible. The present invention aims to provide a cell blank measurement method that can perform cell blank measurement on a reaction vessel in which a reaction has already progressed.

0010

[Means for Solving the Problems] In order to achieve the above object, the present invention is based on an automatic analyzer configured to clean a reaction container for reuse, and to store a sample in the reaction container. and reagents are dispensed and optically analyzed and measured at a predetermined wavelength, and after injecting washing water into the reaction vessel in which the optical measurement has been completed, the reaction vessel into which only this washing water has been injected is transferred to an optical measurement device. In this optical measurement device, the cell blank value is measured at the same wavelength as the predetermined wavelength used for the optical analysis measurement, and if the washing water overflows in the washing stage and washing becomes impossible, the analysis has already been carried out. The configuration is configured to automatically save all wavelengths of the cell blank value of the reaction vessel before the reaction has finished, and by using this saved cell blank value of all wavelengths, the cell blank value for the reaction vessel in which the reaction has already progressed can be saved. It is characterized by being configured to perform blank measurements.

[0011]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described in detail below based on an embodiment shown in the accompanying drawings.

FIG. 1 shows an example of a schematic configuration of an automatic analyzer to which the cell blank measuring method according to this embodiment is applied. 1 holds a reaction vessel 2 in a loop shape, and by rotating this reaction table 1 at a predetermined timing in the direction of the arrow in the figure by 360 degrees ± 1 pitch, each reaction vessel 2 is moved from the sampling position S to the first position. The first reagent dispensing position R1 is sent to the stirring position K1, and the reaction vessel 2 whose stirring operation has been completed at the stirring position K1 is further sent from the second reagent dispensing position R2 to the optical measurement position D via the stirring position K2. After that, optical analysis work using a predetermined analysis wavelength is performed at the optical measurement position D, and then the reaction vessel 2, which has undergone the measurement work at the optical measurement position D, is sent to the cleaning position 7 and is thoroughly cleaned. The reaction vessel 2 is configured to be delivered to the sampling position S again.

At the optical measurement position D, the reaction table 1 is
While rotating by 60 degrees ± 1 pitch, optical measurements of all reaction vessels 2 held on the reaction table 1 are performed. In this case, since the measurement light emitted from the light emitting device 3 has all wavelengths, the measurement light transmitted through each reaction vessel 2 is spectrally divided into wavelengths corresponding to the analysis items by a known light receiving device 4 using a diffraction grating. The cell blank value of the reaction vessel 2 which has been processed and contains washing water is measured.

FIG. 2 shows an example of processing of cell blank values in the automatic analyzer configured as described above.

In the figure, reference numeral 10 denotes a microprocessor device, 11 denotes a container identification unit for identifying the reaction vessel 2 that has been cleaned by the cleaning device 7, and 12 denotes an absorbance measuring unit for measuring the sample absorbance input from the light receiving device 4. , 13 is a wavelength data memory that stores the measurement wavelength for each reaction container 2 used for measuring the sample absorbance, and 14 is a container identification number input from the container identification section 11 and the corresponding container input from the wavelength data memory 13. 15 is a cell blank measurement unit that causes the light emitting device 3 and the light receiving device 4 to perform cell blank measurement based on the wavelength data of the light receiving device 4; Section 16 is an absorbance correcting section that corrects the absorbance by canceling the cell blank value for the container from the absorbance data input from the absorbance measuring section 12. Also,
Reference numeral 17 is a display device such as a CRT that displays the absorbance data obtained by the absorbance correction section 16, and 18 is a printer device that prints the absorbance data.

[0016] In the device configured as described above,
If we focus on one particular reaction vessel 2 and explain its routine work process, as shown by the solid line in FIG. 2, when this reaction vessel 2 reaches the sampling position S,
After the measurement wavelength for the reaction container 2 (sample in the reaction container) is specified by the microprocessor device 10,
A sample and first and second reagents are dispensed into the reaction container 2 at each position.

When the reaction container 2 into which the sample and the first and second reagents have been dispensed in this way is sent to the optical measurement position D, the container identification section 11 identifies the identification number of the reaction container 2 at the position D. is recognized, and optical analysis measurement using the measurement wavelength specified at the sampling position S is performed by the optical device 3 and the light receiving device 4.

The light receiving voltage signal thus inputted from the light receiving device 4 is stored as absorbance data in the absorbance measuring section 12, and the measured wavelength is stored in the wavelength data memory 13.

After that, the reaction container 2 after the optical measurement is sent to the cleaning device 7, and when washing water is injected into the reaction container 2 at the final stage of the cleaning device 7, the reaction container 2 is By rotating the reaction table 1 360 degrees ± 1 pitch as described above, the washing water is sent to the next drainage process.
Since the reaction container 2 passes through the optical measurement position D during the rotation of the reaction table 1 by 360 degrees ±1 pitch, the cell blank value is measured by the optical device 3 and the light receiving device 4.

That is, in this cell blank value measurement, the measurement light irradiated from the light emitting device 3 has a full wavelength as in the optical analysis measurement, but at this time, the cell blank command section 14 controls the container identification section. Based on the identification number data from 11 and the wavelength data from the wavelength data memory 13, only the cell blank value of the wavelength data portion that is the same as the optical analysis wavelength of the reaction vessel 2 is picked up, and the cell blank measurement unit 15
input and store it. Of course, when the optical analysis wavelengths of the reaction vessel 2 are two specific wavelengths, the wavelengths at which the cell blank values are measured are also the same two specific wavelengths, and the average value of the data measured at these two specific wavelengths is the cell blank value. value.

When the cell blank value is determined in this manner, the absorbance correction section 16 corrects the analytical absorbance of the sample based on the cell blank value. The calculation method for this correction is the same as that for conventional cell blank correction, so detailed explanation thereof will be omitted here.

The corrected absorbance obtained in this way becomes the true absorbance data for each specimen, so the data is displayed on the CRT 17 or printed by the printer device 18.

Furthermore, if the cleaning water overflows in the cleaning stage due to a failure of the cleaning device 7, and cleaning becomes impossible, a signal from the overflow detection sensor 8 provided in the cleaning device 7 is sent to the cell blank command section. 14, and when the signal is input, the cell blank command unit 14 automatically sends all wavelengths of the cell blank value of the previous reaction vessel 2, which has already been analyzed, to the cell blank measurement unit 15. command to save.

[0024] Previous reaction vessel 2 stored in this way
The cell blank value of all wavelengths for
The absorbance correction unit 16 corrects the analytical absorbance for the reaction vessel 2 in which the reaction has already progressed based on the average value of the cell blank values for all wavelengths. Of course,
The absorbance correction unit 16 picks up only the cell blank wavelength necessary for the reaction vessel 2 in which the cell blank measurement is performed from among the stored cell blank values of all wavelengths in the previous reaction vessel 2, and uses this. In this way, the analysis absorbance can be corrected for the reaction vessel 2 in which the reaction has already progressed.

The corrected absorbance thus obtained is displayed on the CRT 17 or printed by the printer 18 along with a display indicating that the cell blank measurement is due to overflow.

In the above embodiment, the case where the wavelength data memory 13 is provided in the microprocessor device 10 has been explained as an example, but the present invention is not limited to this. If analysis wavelength data for each container is stored in a host computer, the analysis wavelength data of this host computer can be used without providing a separate wavelength data memory.Furthermore, the CRT 17 and printer device 18 can It is provided as needed, and the corrected absorbance data can be stored in an external storage device such as a floppy disk.

Of course, in the present invention, the light emitting device 3 can also be constituted by a known filter wavelength conversion device.

[0028]

Effects of the Invention As explained above, according to the cell blank measurement method according to the present invention, after each reaction container has been cleaned, the cell blank value can be measured using the same wavelength as the analysis wavelength for each reaction container. As a result, it is possible to completely eliminate correction errors caused by the difference between the analysis wavelength and the cell blank measurement wavelength, greatly improving the reliability of measurement accuracy, and also In addition to the effect of improving the drift correction effect because the value measurement point is closer, even if the washing water overflows in the washing stage due to a failure of the washing equipment and washing becomes impossible, the reaction has already progressed. Since the cell blank can be measured for the reaction vessel in which it is currently in use, excellent effects such as significantly improving the reliability of the measurement work can be achieved.

[Brief explanation of the drawing]

FIG. 1 is an explanatory plan view showing a schematic configuration of an automatic analyzer according to an embodiment of the present invention.

FIG. 2 is a circuit diagram showing an example of a cell blank processing circuit of an automatic analyzer according to an embodiment of the present invention.

[Explanation of symbols]

2 Reaction container 3. Light emitting device 4 Light receiving device 7　Cleaning device 8 Overflow detection sensor 11 Container identification section 12 Absorbance measurement section 13 Wavelength data memory 14 Cell blank instruction section 15 Cell blank measurement section 16 Absorbance correction section

Claims (1)

[Claims] Claim 1: An automatic analyzer configured to clean a reaction container for reuse, which dispenses a sample and a reagent into the reaction container, performs optical analysis measurement at a predetermined wavelength, and performs optical measurement at a predetermined wavelength. After injecting washing water into the completed reaction container, the reaction container into which only this washing water has been injected is transferred to an optical measurement device, and in the optical measurement device, the wavelength same as the predetermined wavelength used for the optical analysis measurement is measured. In addition to measuring the cell blank value in the above washing stage, if the washing water overflows in the washing stage and washing becomes impossible, the cell blank value for all wavelengths of the reaction vessel that has already been analyzed is automatically saved. A cell blank measurement method characterized in that the cell blank measurement method is configured to perform cell blank measurement on a reaction vessel in which a reaction has already progressed by using the stored cell blank values of all wavelengths.